List of primers described in Goldstein et al. (1999) Yeast 15:507-511 ...
List of primers described in Goldstein et al. (1999) Yeast 15:507-511 ...
List of primers described in Goldstein et al. (1999) Yeast 15:507-511 ...
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<strong>List</strong> <strong>of</strong> <strong>primers</strong> <strong>described</strong> <strong>in</strong><br />
Goldste<strong>in</strong> <strong>et</strong> <strong>al</strong>. (<strong>1999</strong>) <strong>Yeast</strong> <strong>15</strong>:<strong>507</strong>-<strong>511</strong> and<br />
Goldste<strong>in</strong> <strong>et</strong> <strong>al</strong>. (<strong>1999</strong>) <strong>Yeast</strong> <strong>15</strong>:<strong>15</strong>41-<strong>15</strong>53.<br />
Primer Function Sequence<br />
JM7<br />
JM8<br />
JM37<br />
5' targ<strong>et</strong><strong>in</strong>g primer for HO locus<br />
3' targ<strong>et</strong><strong>in</strong>g primer for HO locus<br />
verification primer for MX cass<strong>et</strong>tes, homologous to<br />
term<strong>in</strong>ator (TTEF)<br />
CCTCATAAGCAGCAATCAATTCCATC<br />
TATACTTTAAAGCATAGGCCACTAGT<br />
GGATCTG<br />
CTTTTATTACATACAACTTTTTAAAC<br />
TAATATACACATTTCAGCTGAAGCTT<br />
CGTACGC<br />
CCTCGACATCATCTGCCC<br />
PH7 5' verification primer for <strong>in</strong>tegration at HO locus CGCAAGTCCTGTTTCTATGC<br />
PH8 3' verification primer for <strong>in</strong>tegration at HO locus CTACGTTGCCTCCATCG<br />
PH14<br />
PH<strong>15</strong><br />
5' verification primer for <strong>in</strong>tegration at URA3 locus GTATTGAGAAGGGCAACGG<br />
3' verification primer for <strong>in</strong>tegration at URA3 locus CCAGTACACCTTATCGGC<br />
PR29 Univers<strong>al</strong> cass<strong>et</strong>te construction CACATACGATTTAGGTGACAC<br />
PR30<br />
PR31<br />
natMX cass<strong>et</strong>te construction<br />
natMX cass<strong>et</strong>te construction<br />
GGTAAGCCGTGTCGTCAAGAGTGGTA<br />
CCCATGGTTGTTTATGTTC<br />
GCTCTACATGAGCATGCCCTGCCCCT<br />
AATCAGTACTGACAATAAAAAG<br />
PR32 Univers<strong>al</strong> cass<strong>et</strong>te construction AATACGACTCACTATAGGGAG<br />
PR33 natMX cass<strong>et</strong>te construction ACCACTCTTGACGACACGGCTTACC<br />
PR34 natMX cass<strong>et</strong>te construction GGGGCAGGGCATGCTCATGTAGAGC
PR35<br />
PR36<br />
hphMX cass<strong>et</strong>te construction<br />
hphMX cass<strong>et</strong>te construction<br />
CGTCGCGGTGAGTTCAGGCTTTTTAC<br />
CCATGGTTGTTTATGTTC<br />
CAGCACTCGTCCGAGGGCAAAGGAAT<br />
AATCAGTACTGACAATAAAAAG<br />
PR37 hphMX cass<strong>et</strong>te construction AAAAAGCCTGAACTCACCGCGACG<br />
PR38 hphMX cass<strong>et</strong>te construction TTCCTTTGCCCTCGGACGAGTGCTG<br />
PR43<br />
PR44<br />
patMX cass<strong>et</strong>te construction<br />
patMX cass<strong>et</strong>te construction<br />
CTCAACTGGTCTCCTCTCCGGAGAAC<br />
CCATGGTTGTTTATGTTC<br />
CCAGTTAGGCCAGTTACCCAGATCTA<br />
ATCAGTACTGACAATAAAAAG<br />
PR45 patMX cass<strong>et</strong>te construction TCTCCGGAGAGGAGACCAGTTGAG<br />
PR46<br />
PR59<br />
PR60<br />
PR78<br />
PR79<br />
patMX cass<strong>et</strong>te construction<br />
used for URA3/MX4 ORF switch<strong>in</strong>g (5')<br />
used for URA3/MX4 ORF switch<strong>in</strong>g (3')<br />
upstream primer for MX cass<strong>et</strong>tes homologous to<br />
promoter (PTEF)<br />
downstream primer for MX cass<strong>et</strong>tes homologous to<br />
term<strong>in</strong>ator (TTEF)<br />
TTAGATCTGGGTAACTGGCCTAACTG<br />
G<br />
TTCTCACATCACATCCGAACATAAAC<br />
AACCATGACAGTCAACACTAAGACCT<br />
ATAGTG<br />
AAACAACAATCTTTTTATTGTCAGTA<br />
CTGATTATAATTGGCCAGTCTTTTTC<br />
AAATAAG<br />
CCTTGACAGTCTTGACGTGC<br />
CGCACTTAACTTCGCATCTG
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