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24 3 26 17151915 

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3Characterization of laminin isoforms in the anterior pituitary 

Dini Ramadhani 

 

TEL0285587313FAX0285445243Emailtyashiro@jichi.ac.jp 

 

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24 3 26 17151915 

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117

117 25 

 

 

:: 

 

 

 

 

:: 

 

 

GlialAxon Interactions in Health and Disease 

Bruce D. TrappDepartment of Neurosciences, Lerner Research Institute, Cleveland Clinic, USA 

::C K 

 

 

 

 

::D K 

 

 

 

 

::A M 

 

 

 

SM G 

::A M

26 

117 

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15 

 

 

30 

 

 

::A M 

 

 

Dual Beam

117 27 

 

 

::A M M 

S. Neuronal death 

S 

S 

S 

S 

 

 

Physiological roles of cell death signaling in mouse neural development 

Masayuki Miura 1,2 1 2 CREST, JST 

Characterization of Early Pathogenesis in the SODGA Mouse Model of ALS. 

Carol Milligan, Sherry Vinsant, Carol Mansfield, Masaaki Yoshikawa, Ramon Moreno, David Prevette, 

Ronald OppenheimDepartment of Neurobiology and Anatomy, and the ALS Center, Wake Forest School of Medicine, 

WinstonSalem, NC 27157, US 

Nerve injury induced motor neuron death with reference to neuronglia interaction 

Hiroshi KiyamaDept. Functional Anatomy & Neurosci., Nagoya Univ. Grad. Sch. Med. 

Autophagy in the neural network and its impairment 

Yasuo UchiyamaDept of Cell Biol & Neurosci, Juntendo Univ Grad Sch of Med 

::B M M 

S. 

S 

S 

S 

S 

S 

 

 

Rab 

 

 

 

 

 

 

 

 

1,2 1 1 2 JST 

::C K K 

S. 

 

S 

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S

28 

S 

S 

S 

S 

117 

 

 

ex vivo 

 

 

 

 

1,2 1 2 

::D K K 

S. 

S 

S 

S 

S 

S 

 

 

 

 

The role of autophagyrelated proteins on lipid metabolism in the ER membrane 

Taki Nishimura 1 , Anoop Kumar Velikkakath 1 , Naotada Ishihara 1,2 , Eiko Oita 1 , Noboru Mizushima 1 

1 Dept. Physiol. Cell Biol., Tokyo Med. Dent. Univ., 2 Dept. Protein Biochem., Inst. Life Sci., Kurume Univ. 

ApoB 

 

DGK 

 

 

 

::E L C LC 

S. 

 

S 

1,2,3 1,2 1,2 1 1 2 3 CREST 

S 

 

S Ca + 

1 1 2 1 2 2 1 

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S 

 

S

117 29 

::F L C LC 

S. 

S 

S 

S 

S 

 

 

 

 

 

 

 

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1 2 3 

 

 

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S. D 

S 

S 

S 

S 

S 

 

 

 

 

 

 

 

 

 

 

 

 

::H L C LC 

S. 

 

S 

 

S 

 

S 

 

S 

Brant WeinsteinNational Institutes of Health, NICHD

30 

S 

117 

Coordinated branching morphogenesis of coronary vessels and peripheral sympathetic nerves in the 

developing heart 

Yosuke Mukoyama 1,2 1 National Institutes of Health, 2 National Heart, Lung, and Blood Institute 

::B M M 

S. 

S 

S 

S 

S 

S 

 

 

CT 

 

 

1 2 1 1 1 2 2 1 

2 

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5 

::C K K 

S. 

 

 

S Underlying mechanism that generates a phase wave in the mammalian circadian center 

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S 

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S 

 

::D K K 

S. 

II 

S 

II

117 31 

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32 

117 

 

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117 33 

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S BFI FIB/SEM 

1 2 2 1 1 1 1 

1 2 

S 

1 2 1 2 1 

1 2 2 1 

S Motile and immune functions of intestinal subepithelial myofibroblasts 

Islam Md Shafiqul, Hiroshi Ozaki 

Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The university of Tokyo 

S 

 

::B M M 

S. 

 

 

S 

 

S PBL 

 

S 

 

S 

 

S 

 

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117 35 

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S. 

 

 

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S 

 

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S 

 

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3 

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1 1,2,3 4 4 5 2 1,2,3 

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S. 

 

 

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1 1 2 1 2 

S 

1,2 1 1 

1 2

36 

117 

S kisspeptin 

 

S 

 

S 

 

::F L C LC 

S. 

 

 

S 

Fumiyo Aoyama, Nobuyasu Takahashi, Soyuki Ide, Akira Sawaguchi 

 

S 

1,2 1 1 1 2 

S 

 

S Focused Ion BeamScanning Electron Microscopy FIBSEM Live imaging 

 

1,2 1 2 Department of Neurosciences, 

Lerner Research Institute, Cleveland Clinic 

::G L C LC 

S. 

 

 

S 

 

S CEACAM 

 

S 

 

S Implications of histone modification and DNA methylation during mouse spermatogenesis 

Ning Song, Takehiko Koji 

Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences 

S Unique integration of fertilizationrelated protein Equatorin EQT into the acrosomal membrane 

during spermatogenesis 

Chizuru Ito, Kenji Yamatoya, Kiyotaka Toshimori 

Dept. Anatomy and Developmental Biology, Grad. Sch. Med., Univ. Chiba

117 37 

::H L C LC 

S. 

 

 

S 

 

S 

 

S 

 

S 

 

S 

 

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S. 

 

 

S 

1 2 1 2 

S MRI MRTD 

1,2 2 1 3 4 4 1 

2 

3 4 

S Virchow 

 

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S 

1,4 2 3 1 Brant Weinstein 4 

1 2 3 

4 Section on Vertebrate Organogenesis, NICHD, NIH 

::A M M 

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38 

117 

S 

 

S 

 

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S. 

 

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S 

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5 

6 MRC 

S Recurrent connection selectivity of layer V 

pyramidal cells in frontal cortex 

1,2 1,2 1 2 

S A Hz Oscillation Synchronizes Prefrontal, VTA, and Hippocampal Activities during Working memory 

Gyorgy BuzsakiRutgers University 

S Development of orientation and direction selectivity in the mouse visual cortex 

1,2 Nathalie Rochefort 2 Christine Grienberger 2 Nima Marandi 2 Daniel Hill 2 Arthur Konnerth 2 

1 2 Inst. Neuroscience, Technical Univ. Munich

117 39 

 

 

::I L C LC 

W. 

W 

W 

W 

W 

W 

W 

 

 

WG 

1,2 

1 2 

 

1 1,2 

1 2 

 

1 2 1 2 

 

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1 2 M2 3 M4

40 

117 

 

 

E L C LC 

::OEPM I 

 

 

1OEPMI1 Singleneuron tracing study of thalamocortical projections arising from the rat mediodorsal 

nucleus 

1OEPMI2 V 

1OEPMI3 

1OEPMI4 GABA 

 

::OEPM II 

 

 

1OEPMII1 BAF dpf1 GFP 

 

1OEPMII2 mRNA 

1OEPMII3 Immunocytochemical and proteomic analysis of the γsecretase complex using the 

conformationspecific antiNicastrin antibody 

F L C LC 

::OFPM I 

1 

 

1OFPMI1 Rho ROCK1, ROCK2 

1OFPMI2 Photoactivatable Rac1 

1OFPMI3 GaAsP LIVE 

 

1OFPMI4 229E CD13 

 

::OFPM II 

 

 

1OFPMII1 Nphp3 

1OFPMII2 

1OFPMII3 PACAP 5 

G L C LC 

::OGPM I 

 

 

1OGPMI1 microCTmicroMRI

117 41 

1OGPMI2 

 

1OGPMI3 HyperDry 

1OGPMI4 

::OGPM II 

 

 

1OGPMII1 

1OGPMII2 Muse iPS 

1OGPMII3 Monitoring DNA damage levels using γH2AX detection in vivo 

H L C LC 

::OHPM 

 

 

1OHPM1 

1OHPM2 MS5 

1OHPM3 

1OHPM4 Two immunogenic passenger DC subsets in the rat liver with a distinct trafficking pattern and 

radiosensitivity 

::OHPM 

 

 

1OHPM5 

1OHPM6 

1OHPM7 

I L C LC 

::OIPM 

 

 

1OIPM1 α 4 

1OIPM2 L LFABP 

1OIPM3 EDA 

::OIPM 

 

 

1OIPM4 Kiyotaka Toshimori Fertilization analyzed by live imaging in the mouse 

1OIPM5 

1OIPM6 microRNA

42 

117 

 

E L C LC 

::OEAM III 

 

 

2OEAMIII1 

2OEAMIII2 NPY 

2OEAMIII3 

 

2OEAMIII4 gastrinreleasing 

peptide 

::OEAM IV 

 

 

2OEAMIV1 TRPV4 

2OEAMIV2 Hh 

2OEAMIV3 AP1 Notch 

::OEAM V 

 

 

2OEAMV1 MAP1A supports NMDAreceptor transport for synaptic plasticity 

2OEAMV2 Homer1a regulates the activityinduced remodeling of synaptic structures in cultured 

hippocampal neurons 

2OEAMV3 PIPKα regulates neuronal microtubule depolymerase KIF2A and suppresses elongation of 

axon branches 

F L C LC 

::OFAM III 

 

 

2OFAMIII1 SNAP23 

2OFAMIII2 

2OFAMIII3 

2OFAMIII4 Input pathway and target cell typedependent regulation of synaptic AMPAR subunits in 

hippocampal CA1 region 

::OFAM IV 

 

 

2OFAMIV1 

2OFAMIV2 

2OFAMIV3 

::OFAM V 

 

 

2OFAMV1 BMP2 dexamethasone

117 43 

2OFAMV2 

2OFAMV3 

G L C LC 

::OGAM I 

 

 

2OGAMI1 

 

2OGAMI2 

2OGAMI3 

2OGAMI4 

::OGAM II 

 

 

2OGAMII1 Angela QuispeSalcedo 

The application of antimicrobials or glycogen accelerates the pulpal regeneration of replanted 

molars in mice 

2OGAMII2 

2OGAMII3 BrdU 

::OGAM III 

 

 

2OGAMIII1 Cx45 NFATc 

2OGAMIII2 FGF 

2OGAMIII3 An attempt to identify novel ciliary genes by database comparison 

H L C LC 

::OHAM I 

 

 

2OHAMI1 

2OHAMI2 On the phylogeny of the zygomatic arch, and its relationships among foramina and the bony 

wall of the orbit 

2OHAMI3 

2OHAMI4 

::OHAM II 

 

 

2OHAMII1 Nabil Eid Persistent median artery: Relationships to palmar arches and median nerve branches 

2OHAMII2 

2OHAMII3 Thiel 

::OHAM III 

 

 

2OHAMIII1

44 

117 

2OHAMIII2 

2OHAMIII3 

1 

 

I L C LC 

::OIAM I 

 

 

2OIAMI1 CT 

 

2OIAMI2 

2OIAMI3 klotho/ 

2OIAMI4 

::OIAM II 

HBS 

 

2OIAMII1 Osteogenic culture 

 

2OIAMII2 PKR 

2OIAMII3 WT1 RNA 

::OIAM III 

 

 

2OIAMIII1 septoclast EFABP 

 

2OIAMIII2 

 

2OIAMIII3

117 45 

 

D K LC 

::ODPM I 

 

 

3ODPMI1 Large Maf 

3ODPMI2 

 

3ODPMI3 Notch 

3ODPMI4 

::ODPM II 

 

 

3ODPMII1 Dini Ramadhani Expression of laminin isoforms during anterior pituitary development in the rat 

3ODPMII2 

 

3ODPMII3 

 

E L C LC 

::OEPM VI 

 

 

3OEPMVI1 

3OEPMVI2 Developmental Origins of Health and Disease DOHaD 

 

3OEPMVI3 

3OEPMVI4 15q1113 E/I 

::OEPM VII 

 

 

3OEPMVII1 

3OEPMVII2 

3OEPMVII3 RAGE 

 

::OEPM VIII 

 

 

3OEPMVIII1 Fasting and highfat diet alter histone deacetylase expression in the medial hypothalamus 

3OEPMVIII2 

3OEPMVIII3 PAM

46 

117 

F L C LC 

::OFPM VI 

 

 

3OFPMVI1 

2 

3OFPMVI2 Haruki Senoo Biodiversity and hepatic stellate cells 

3OFPMVI3 A 

3OFPMVI4 IEL DNA 

::OFPM VII 

 

 

3OFPMVII1 SU6656 p53 

3OFPMVII2 

3OFPMVII3 HORMAD2 

::OFPM VIII 

 

 

3OFPMVIII1 α1 

 

3OFPMVIII2 E3 HRD1 

3OFPMVIII3 

G L C LC 

::OGPM IV 

 

 

3OGPMIV1 NEX 

3OGPMIV2 FLRT Unc5 

3OGPMIV3 Kyoji Ohyama Implications for cooperative versus noncooperative actions of the subunits of NatB, Mdm20 

and Nat5 in mouse embryonic brain 

3OGPMIV4 

::OGPM V 

 

 

3OGPMV1 Hes1 

3OGPMV2 M. iliotibialis cranialis 

3OGPMV3 

 

::OGPM VI 

 

 

3OGPMVI1 Yoshiro Takano Differential effect of aberrant expression of ectodysplasinA receptor edar on scales and jaw 

and pharyngeal dentition of medaka 

3OGPMVI2 DNA TGFβ3

117 47 

H L C LC 

::OHPM IV 

 

 

3OHPMIV1 

3OHPMIV2 3D 

3OHPMIV3 

3OHPMIV4 

3OHPMIV5 Takamitsu Arakawa Threedimensional modeling of the architecture of the plantar muscles of the great toe 

::OHPM I 

 

 

3OHPMI1 ShineOd Dalkhsuren 

The characteristic of the filiform and fungiform papillae of human tongue 

3OHPMI2 

3OHPMI3 

::OHPM II 

 

 

3OHPMII1 CRF 

3OHPMII2 

I L C LC 

::OIPM IV 

 

 

3OIPMIV1 tendon gel 

3OIPMIV2 

3OIPMIV3 

::OIPM 

 

 

3OIPM1 

 

3OIPM2 CT3 1 

3OIPM3 

::OIPM 

 

 

3OIPM4 Mari Hiraoka Organization of the lens elasticity associating with accommodation in monkey eyes 

3OIPM5 CCP1 

3OIPM6

48 

117 

 

 

:: 

I 

1P001 S100A6 

1P002 Yumiko Matsusue Distribution of corticosteroid receptors in oligodendrocytes of mice 

1P003 4F2 

 

1P004 Jiaorong Chen Immunohistochemical Analyses of Protein 4.1G in Enteric Peripheral Nervous Tissues by “in vivo 

Cryotechnique” 

1P005 in vivo in vitro 

1P006 

1P007 

1P008 

1P009 

1P010 

1P011 1 DCC 

 

1P012 Spock3 null 

1P013 4 5 γ 

1P014 

1P015 

1P016 

1P017 

1P018 WNPW 

1P019 Kinya Kubo Chewing under restraint stress inhibits the stressinduced suppression of cell proliferation in the 

hippocampal dentate gyrus 

1P020 Kouko Tatsumi Voluntary exercise promotes astrogliogenesis from Olig2 cells in some nuclei of the basal ganglia of 

adult mouse 

1P021 GDNF mRNA 

1P022 II 

1P023 

1P024 cfos 

1P025 cFos 

1P026 

1P027 

1P028 / 

2 

1P029 n3 

1P030 Shinji Tanaka Altered dynamics of the cortical neuronal circuit in a mouse model of autism 

1P031 Miwako MasugiTokita 

Mechanism of deficit in aggressive behavior of metabotropic glutamate receptor subtype 7 knockout 

mice 

1P032 PTPRA 

1P033 Kisspeptin

117 49 

1P034 

1P035 

1P036 TGFβ1 

1P037 Hiroaki Okuda Identification of CSPG constituting DACS, a novel brain extracellular matrix 

1P038 

1P039 Mutations in POLR3A and POLR3B encoding RNA polymerase III subunits cause an 

hypomyelinating leukoencephalopathy 

1P040 

1P041 Zitter rat 

1P042 

1P043 Stigmoid body 

1P044 

1P045 

I 

1P046 Eusthenopteron foodi 

1P047 

1P048 

1P049 NOS 

1P050 1 

1P051 TNFα/RANKL microRNA 

1P052 hemokinin1 

1P053 DGKζ Retinoblastoma 

1P054 

1P055 PACAP VIP PACAP 

VIP 

1P056 5 

1P057 gustducin 

1P058 

1P059 

1P060 APC Min/+ 

1P061 DPEP1 

1P062 Md Moksed Ali Placenta specific miR-517a modulates gene expression in Jurkat cells 

1P063 microRNA CpG 

1P064 IgG IIb Fc FcRIIb 

 

1P065 Yoshiya Asano Agerelated accumulation of nonheme ferric and ferrous iron in mouse ovarian stroma visualized by 

sensitive iron histochemistries 

1P066 

1P067 

 

1P068 TG 

1P069 

 

1P070 

1P071 2 

1P072

50 

117 

1P073 Makoto Naganuma Activities of human upper limb muscles during a combined movement of elbow flexion/extension and 

forearm pronation/supination 

1P074 

1P075 

 

1P076 

1P077 

1P078 

1P079 

1P080 

1P081 

1P082 

1P083 

1P084 

1P085 

1P086 

1P087 Persistent left hepatic venous directly opening into the right atrium 

1P088 

1P089 

1P090 

 

1P091 

1P092 

1P093 

1P094 LEH 

1P095 

1P096 

1P097 Nacholapithecus kerioi 

 

1P098 Involvement of Olig2 in the formation of reciprocal connection between the thalamus and cortex 

1P099 Studying nascent daughter cells’ neighborship to understand the mechanism of cell fate choices in the 

neocortical neurogenesis 

1P100 GABAA 

1P101 

1P102 kirrel3 

1P103 Characterization of the RNAbinding protein Musashi1 in zebrafish 

1P104 PSANCAM 

1P105 mRNA 

1P106 CNTF 

1P107 

1P108 

1P109 kirrel3 

1P110 

1P111 / Sprouty4 

1P112 

1P113 

1P114 Dlg1 

1P115 sox9

117 51 

1P116 Protocadherin 10a 

1P117 Developmental Origins of Health and Disease DOHaD 

 

1P118 α 

1P119 Occludin p63 

1P120 

1P121 

1P122 

1P123 

1P124 LEF1 SP6 

1P125 

1P126 TTF1 

1P127 Keishi Otsu The role of Rho signaling pathway in dental epithelial stem cells 

1P128 Ji Youn Kim Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats 

1P129 Esrat Jahan Fetal jaw movement affects molecular cascade in the development of mandibular condylar cartilage 

1P130 

1P131 

 

1P132 

1P133 FIB/SEM 

 

:: 

II 

2P001 Mohammad Rabiul Karim 

Molecular sequence and distribution of the NMDA receptor subunit NR1 mRNA in the central nervous 

system of pigeons Columba livia 

2P002 2 

2P003 SULT2B1a 

2P004 

2P005 Jahan Mir Rubayet 

Androgen receptor expression in the preoptic and anterior hypothalamic areas of the adult male rats 

and mice 

2P006 GABA parvalbumin 

2P007 SNARE 

2P008 GABA 

2P009 Expression of AMPA type glutamate receptor in developing chick vestibulocochlear ganglia 

2P010 

 

2P011 C 

2P012 

2P013 

2P014 

2P015 

2P016 CART 

2P017 GABA

52 

117 

2P018 Conserved properties of dendritic trees in four cortical interneuron subtypes 

2P019 Majid Ebrahimi FABP7 in astrocytes is involved in control of neuronal dendritic formation 

2P020 FILIP 

2P021 CA1 

2P022 In vivo analysis of postsynaptic molecular dynamics in the developing mouse cortex 

2P023 Ayako Hayashi Direct monitoring of AMPA receptor recycling and trafficking 

2P024 Motor protein KIF1A is essential for hippocampal synaptogenesis and learning enhancement in an 

enriched environment 

2P025 Zheng Huang Activationspecific Changes of Angiotensin II Receptors in Mouse Cerebellum and Adrenal Glands 

with In Vivo Cryotechnique 

2P026 Toshiko Tsumori Nodose ganglion cells expressing melanocortin4 receptor send their fibers to the pancreatic islets in 

the mouse 

2P027 Runx1 

2P028 A 

2P029 Features of boutons distribution along axons of neurons in the caudal nucleus of tractus solitarius of 

the rat 

2P030 Calbindin 

2P031 GALP 

2P032 Hitoshi Kawano Origins of nitric oxide pathways to the median preoptic nucleus 

2P033 Kisspeptin Tuberoinfundibular dopamine TIDA 

2P034 

2P035 CA1 

2P036 

2P037 Classification of rat pallidofugal projection systems revealed by singleneuron tracing study with a 

viral vector 

2P038 A Morphological Analysis of Thalamocortical Axon Fibers of Rat Posterior Nuclei: A Single Neuron 

Tracing Study with Viral Vectors 

2P039 Morphological analysis of axon collaterals derived from single corticospinal neurons in subcortical 

structures 

2P040 

2P041 Projections from the amygdaloid anterior basomedial and anterior cortical nuclei to MCHcontaining 

hypothalamic neurons of the rat 

2P042 Inhibitory inputs of CCKpositive neurons to PVexpressing neurons in mouse neocortex 

2P043 Elimination of somatic climbing fiber synapses proceeds with the differentiation of cerebellar 

interneurons 

2P044 Tectothalamic inhibitory neurons in the inferior colliculus receive converged axosomatic excitatory 

inputs from multiple sources 

2P045 Axon terminals of the corticocollicular projection in the rat auditory system 

2P046 Afferent projection to amygdaloid subnuclei and intrinsic connection of each subnuclei 

II 

2P047 

2P048 

2P049 Toshiyuki Oda Communication between Flagellar Outer and Inner Dynein Arms 

2P050 Toshiki Yagi A novel protein complex required for the formation of microtubule square lattice in green tree frog 

sperm 

2P051

117 53 

2P052 

Tomonori Naguro Mitochondria form continuous intracellular networkstructures: a study visualized by highresolution 

scanning electron microscopy 

2P053 

2P054 

2P055 

 

2P056 VAMP5 

2P057 

 

2P058 Involvement of Rab35 in phagosome formation through regulating ARF6 activity by ACAP2 

2P059 Atg9AmRNA HeLa / A study on Atg9AmRNAknockdown 

HeLa cells 

2P060 mAtg9AAcGFP mDFCP1mCherry 

2P061 

Hironobu Nakane Histological analysis of adipose tissues in Xpg null mice 

2P062 mdx 

2P063 McARH 7777 ALP 

 

2P064 

2P065 lamin A/C 

2P066 ABCG2 

2P067 

 

2P068 DGKε 

2P069 DGKζ NAP DGKζ 

2P070 Cellular localization of ERα and their apoptotic effects 

2P071 

2P072 αMangostin 

2P073 Sevoflurane rat Per2 

 

2P074 

Khairani Astrid Feinisa 

Transverse anchoring system of myofibril to sarcolemma: the morphological study 

2P075 

2P076 Prosaposin expression in cardiac muscle of mdx mice in early period of disease 

2P077 

2P078 

2P079 

2P080 Thiel 

 

2P081 ATDC5 Notch14 PCNA 

2P082 Nox 

2P083 

2P084 

2P085 

2P086 CT CT 

 

2P087 

2P088 

2P089

54 

117 

2P090 

2P091 

2P092 Klotho 

2P093 FAM20A 

2P094 GABA GABA 

2P095 Polypterus senegalus Oxydoras niger 

 

2P096 planar cell polarity 

 

2P097 Miyuki Yamamoto 5 

2P098 Kannika Adthapanyawanich 

Studies on the submandibular gland of androgen receptordeficient mice 

2P099 

2P100 

2P101 3A 3AB 

2P102 

2P103 M Epidermal type FABP EFABP/FABP5 

 

2P104 A 

2P105 cKIT 

2P106 ICCSP 

2P107 CCK1 

2P108 Mayako Morii The metabolism of cholesterol after bile duct degeneration in lamprey 

2P109 NAFLD 

 

2P110 Deltalike3 

2P111 

 

2P112 CEACAM1 

2P113 Masahiro Miura The role of peritoneal lymphatic system on the human peritoneal dissemination 

2P114 

2P115 

2P116 Masataka Sunohara The role of hematopoietic factors and Wnt signaling during tooth development 

2P117 

2P118 podoplanin 

2P119 

2P120 

2P121 

2P122 

2P123 

2P124 Nkx2.5 

2P125 Hideyuki Tanaka Morphological analysis of effects with arachidonic acid in smooth muscle cells 

 

2P126 FABP7 

2P127

117 55 

2P128 Functional Analysis of Nucleoprotein diet from salmon testis, Shirako via Intestinal IgA secretion and 

TLR9 stimulation 

2P129 Musha Muhetaerjiang 

The effects of adjuvants on autoimmune responses against testicular antigens in mice 

2P130 

2P131 Kuerban Maimaiti Germ cell death in testicular autoimmunity 

2P132 

2P133 

2P134 trafficking 

 

:: 

III 

3P001 Tomiko Yakura Alterations of synaptic transcytosis induced by ethanol exposure: apocrinelike structure in the rat 

3P002 LAMP2 

3P003 Souichi Oe Synergistic interaction between Golgi outposts and RNA granules in postlocal translational secretory 

pathway 

3P004 ARF6 BRAG2/IQSEC1 

3P005 Arf6 EFA6A 

3P006 Roles of BMP signaling in synapse development 

3P007 Roles of ACF7, a large linker protein interacting with both microtubules and Factin, in the 

postsynaptic functions 

3P008 Imaging dynamics of axonal mitochondria 

3P009 ERRγR 

3P010 Agerelated changes in estrogen receptorβ mRNA expression in male rat brain 

3P011 PRLR 

 

3P012 

3P013 Md. Nabiul Islam Sporadically lurking HAP1immunoreactive cells in the hippocampus and their morphological relation 

with steroid receptors 

3P014 GR 

3P015 

3P016 GALP 

3P017 CGRP 

 

3P018 SSRI 

3P019 

 

3P020 

3P021 

3P022 PACAP 

3P023 

Takehiko Sunabori Protective effects of hyaluronan tetrasaccharide on hippocampal pyramidal neurons in neonatal mouse 

after hypoxicischemic injury 

3P024 

3P025 The Effects of an 18mer Peptide of Prosaposin in the Attenuation of MPP+/MPTP Toxicity in Vitro 

and in Vivo 

3P026 GABAA JM1232

56 

117 

3P027 

3P028 ABC 

3P029 

3P030 PACAP 

3P031 PMES2 

3P032 Müller 

3P033 

3P034 

3P035 

 

3P036 2 15 

3P037 Masako Nakanishi Involvement of acidic microenvironment on the cancerinduced bone pain 

3P038 Mineo Watanabe IL1β in trigeminal nucleus caudalis contributes to extraterritorial allodynia/hyperalgesia following a 

trigeminal nerve injury 

3P039 cFos 

3P040 Minocycline 

3P041 ITAM KO 

3P042 Zitter 

3P043 

 

3P044 Scaffold attachment factor B SAFB1 and SAFB2 synergistically inhibit intranuclear mobility and 

function of ERα 

3P045 Estrogen αfetoprotein 

3P046 

3P047 

3P048 

 

3P049 manserin 

 

3P050 5βreductase 

3P051 

3P052 PACAP 

3P053 

3P054 Mash1 AADC GAD67 

3P055 P 

3P056 3A3B 

3P057 

3P058 

3P059 

 

3P060 

3P061 

3P062 LPS 

3P063 

3P064 Kenji Yamatoya Separatration of early stage acrosome reacted sperm and analyses of the proteins 

3P065 Cell adhesion molecule1 Nectin3 

3P066 SF1

117 57 

3P067 Autoimmune responses induced by immunization with xenogenic testicular germ cells alone 

3P068 Di2ethylhexyl phthalate 

3P069 Yuki Ogawa The effect of cadmium on immunoenvironment in the testis 

3P070 TIRF 

3P071 PACAP TAC1 

3P072 3 in vitro 

3P073 Ariful Islam Localization of fatty acid binding protein in mouse placenta and its possible role of fatty acid transport 

through trophoblasts 

 

3P074 Kardasewitsch Verocay 

 

3P075 MAP 

3P076 

3P077 Masahiko Kawagishi 

In vivo labeling of halogenated volatile anesthetics via intrinsic molecular vibrations using nonlinear 

Raman spectroscopy 

3P078 Fluorescence Immunohistochemistry by Confocal LSM for Studies of SemiUltrathin Specimens of 

Epoxy ResinEmbedded Samples 

3P079 / 

 

3P080 

 

3P081 GP 

3P082 

3P083 iPad 

3P084 eLearning 

3P085 

3P086 

3P087 

3P088 

3P089 

3P090 

3P091 

3P092 MRI 

3P093 4 

3P094 

3P095 

 

3P096 

3P097 Fossa 

3P098 

3P099 

3P100 Thiel 

3P101

58 

117 

 

 

:: 

3P102 

3P103 

3P104 Kenji Ohata Tubulin polyglutamylation regulates cytoskeletal distribution in brain 

3P105 KCC2 

3P106 

3P107 

3P108 neuroligin1 

3P109 

3P110 1 

3P111 

3P112 

3P113 

3P114 

3P115 

3P116 1 

3P117 

3P118 

3P119 2 

3P120 1 

3P121 

3P122 

3P123 1 5 

3P124 1 

3P125 2 

3P126 KUHWrat 

3P127 9 

3P128 

3P129 

3P130 2 

3P131 myosinIIA 

3P132 FABP5

117

117 59 

 

 

 

 

100 μm 20 nm 

1 μm 60 

50 [1] 

60 

 

 

1970 1999 20 2 

 

 

2005 

 

 

 

[2]10 

DNA 

 

2011 

 

60 

2006 

 

 

[1] R. Danev and K. Nagayama, “Transmission Electron Microscopy with Zernike Phase Plate”, Ultramicroscopy 88 (2001) 

243-252. 

[2] Rochat R H, Liu X, Murata K, Nagayama K, Rixon F and Chiu W, “Seeing the Genome Packaging Apparatus in Herpes 

Simplex Virus type I (HSV-1) B-capsids”, J. Virology, 85 (2011) 1871-1874.

60 

117 

 

GlialAxon Interactions in Health and Disease 

Bruce D. TrappDepartment of Neurosciences, Lerner Research Institute, Cleveland Clinic, USA 

The interaction between myelin forming cells and axons is one of the best described examples of cellcell 

interactions in mammals. Myelin plays multiple roles essential for normal nervous system function. Best 

known is the insulation of axons to permit rapid propagation of nerve impulses by saltatory conduction. By 

concentrating voltage gated sodium channels at nodal axoplasm myelin also saves energy. To attain similar 

conduction speeds of myelinated axons, unmyelinated axons would have to have diameters 100 times larger 

than myelinated axons. Myelin, therefore, also conserves space. One of the most clinically relevant functions 

of myelin is provide trophic support essential for axonal survival. This function reflects the fact that axons 

can be meters away from the neuronal cell body and has to rely on glia support rather than on neuronal gene 

transcription to respond to many local changes in environment. Since axonal degeneration is the major cause 

of neurological disability in primary diseases of myelin, we have been investigating the mechanisms by which 

myelin provides trophic support to axons. The purpose of this presentation is to summarize these studies with 

an emphasis on morphological studies of normal and abnormal myelin-axon interactions. Myelin is essential 

for long-time axonal survival. This is best illustrated by the primary axonal degeneration that develops in 

mice null for the myelin proteins MAG, PLP or CNP. The precise mechanisms by which myelin stabilizes the 

axon is poorly understood. Two general features are common to the axonal pathologies that precede axonal 

degeneration: the axonal cytoskeleton is abnormal and the pathologies dominate in paranodal regions. We 

have compared paranodal axoplasm in myelinated, demyelinated and dysmyelinated axons using time lapse 

imaging and 3-dimensional electron microscopy. We detect alterations in transport and distribution of axonal 

mitochondria and smooth endoplasmic reticulum following demyelination and dysmyelination. These data 

suggest that altered ATP production and calcium homeostasis precede axonal degeneration. In addition the 

stability, length and orientation of microtubules which contain increased phospho tau epitopes were detected. 

Alterations in microtubules inhibit axonal transport and result in paranodal axonal swellings due to organelle 

accumulation. We are also investigating molecular mechanisms responsible for mitochondria fission and fusion 

in normal, demyelinated and dysmyelinated axons. These studies are unraveling the cascade of molecular and 

morphological changes that eventually result in axonal degeneration so we can identify therapeutic targets that 

may delay or stop axonal degeneration in primary diseases of myelin.

117 61 

 

 

 

 

12 

345 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2 

2000 

 

 

 

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22 4 

 

 

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10 K 

 

 

10 K 

 

 

 

 

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1 

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150 9132002

117 63 

 

 

SM G 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

A B C

64 

117 

 

 

 

 

iPS 

 

 

GFP 

 

 

1981 1983 NIHFogarty International Fellow, NINCDS 

QFDE1984 1992 

QFDE 

1992 

 

 

Virchows Arch., 427:5195271996 

Biomed. Rev., 15: 1192004 

 

 

 

 

 

J. Electron Microsc., 59: 

395408201021 

2007 

 

20072346712008 

 

 

Ii 

iiiiiQdot 

IIIII 

iii

117 65 

S 

Physiological roles of cell death signaling in mouse 

neural development 

Masayuki Miura 1,2 

1 

2 CREST, JST 

Caspases function as regulatory molecules not only in cell death, 

but also in non-apoptotic functions. Live-imaging analysis with the 

mouse embryos expressing SCAT3, a fluorescent indicator protein to 

monitor caspase-3 activation in living cells, revealed that preventing 

apoptosis by inhibition of caspases disturbed smooth morphological 

changes of developing neural plates and also reduced the speed of the 

cranial neural tube closure. 

A considerable number of olfactory sensory neurons (OSNs) in 

the developing olfactory epithelium exhibited caspase-3 activity 

without the apoptotic changes of nuclei. This prompts us to assess 

whether caspases exert non-apoptotic functions in OSNs during 

olfactory development. Apaf-1/caspase-9-mediated signaling causes 

the cleavage of a membrane-anchored member of the semaphorin 

family of guidance proteins, Sema7A, in the axons of OSNs 

during development. Analysis of mutant mice deficient for apaf- 

1 or caspase-9 revealed that this Apaf-1/caspase-9-mediated nonapoptotic 

caspase signaling is important for the development of OSNs 

by affecting axonal pathfinding, synapse formation and maturation 

status. 

S 

Characterization of Early Pathogenesis in the 

SODGA Mouse Model of ALS. 

Carol Milligan, Sherry Vinsant, Carol Mansfield, 

Masaaki Yoshikawa, Ramon Moreno, David Prevette 

Ronald Oppenheim 

Department of Neurobiology and Anatomy, and the ALS Center, 

Wake Forest School of Medicine, WinstonSalem, NC 27157, US 

Charcot first described Amyotrophic Lateral Sclerosis in 1869 

yet, effective, long-term treatment strategies are not available. A 

mouse model was developed after the identification of mutations 

in the SOD1 gene approximately 17 years ago, and most of our 

knowledge of the etiology and pathogenesis of the disease is from 

studies using this model. Although numerous preclinical trials have 

been conducted, results have been disappointing in that they did not 

positively translate in clinical trials. Characterization of the early 

pathological events may provide insight into disease onset, help in the 

discovery of presymptomatic diagnostic disease markers, and identify 

novel therapeutic targets. Many previous studies have identified 

pathological events in the mutant SOD1 models involving spinal 

cord, peripheral axons, neuromuscular junctions or muscle; however, 

few have systematically examined pathogenesis at multiple sites. In 

this study, we examine both central and peripheral components of 

the neuromuscular system in the SOD1G93A mouse model of ALS 

and relate these alterations to early muscle denervation. Our results 

provide insight into the earliest pathological events in this model. 

S 

Nerve injury induced motor neuron death with 

reference to neuronglia interaction 

Hiroshi Kiyama 

Dept. Functional Anatomy & Neurosci., Nagoya Univ. Grad. Sch. 

Med. 

The peripheral nerve injury induces changes not only in the injured 

neurons but also in surrounding glial cells. An interaction between 

neurons and glial cells might be critical in proper regeneration and 

functional recovery. One of the prominent glial behaviors seen after 

motor nerve injury was the microglial migration, proliferation and 

adhesion to the surfaces of injured motor neurons in response to 

nerve injury. Although the consequence of this adhesion between the 

nerve injured motor neurons and microglia seen during the first two 

weeks is not clear, nerve injured motor neurons, which lacked the 

initial tight adhesion with microglia, showed degenerative feature. 

In addition the knockout of a chemokine receptor CCR5, which 

specifically expressed by microglia in response to nerve injury, 

accelerated the motor neuron death. These evidences suggest that the 

microglial behavior seems important perhaps to provide neurons with 

a signal for survival and regeneration. In this symposium I would like 

to address morphological characteristics observed in the nerve injury 

induced motor neuron death and the interactions among injured motor 

neuron and surrounding glial cells. 

S 

Autophagy in the neural network and its impairment 

Yasuo Uchiyama 

Dept of Cell Biol & Neurosci, Juntendo Univ Grad Sch of Med 

Cathepsin D (CD) is distributed in various mammalian cells. We have 

shown that CNS neurons in CD-deficient mouse brains present a new 

form of lysosomal accumulation disease with a phenotype resembling 

neuronal ceroid lipofuscinosis (NCL). The most striking feature of the 

CD-/- CNS neurons is storage of autophagosomes, autolysosomes, 

granular osmiophilic deposits (GRODs), and fingerprint profiles, 

morphological hallmarks of NCLs. Moreover, GRODs are found 

within nascent autophagosomes, indicating that the GROD is a potent 

inducer of autophagy in neuronal cells. Autophagosomes, but not 

GRODs or lysosomes, frequently accumulate in the axon, indicating 

that subsets required for autophagosome formation are present in 

the axon. Until recently, it has been reported that impairment of 

autophagy due to Atg7 or Atg5 deficiency in the axon terminal of 

Purkinje cells induces neurodegeneration. These results indicate that 

not only in autophagy-unable neurons but also in neurons with excess 

of autophagy impairment of autophagy in the axon and its terminal 

induces neurodegeneration. However, the molecular mechanism of 

autophagosome formation in the axon and its terminal largely remains 

unknown.

66 

117 

S 

Rab 

 

 

Rab GTP 

Rab GTP 

RabGAP GDP/GTP RabGEF 

RabGAP TBC 

RabGEF 

 

Rab3 GEF 

MADD DENN domain RabGEF 

DENN 

17 

Rab 

S 

 

 

 

recycling endosomes: REs 

 

 

 

 

evectin2 evectin2 

pleckstrinhomology 

PH domain N PH domain 

REs evectin2 PH domain 

in vitro phosphoinositides 

phosphatidylserine PS 

PS in vivo evectin2 PH 

domain PS PS 

lactadherin C2 domain 

REs PS 

REs 

PS evectin2 

 

 

S 

 

 

 

 

 

 

 

 

AP1 GGAs 

6 

 

 

AP1/ 

GGAs 

 

 

 

AP1 

Notch 

 

EGFR 

AP1 GGA2 EGFR 

 

Notch EGFR 

AP1/GGA 

 

S 

 

 

 

 

 

 

 

 

C. elegans 

C. 

elegans 

 

 

 

 

 

 

 

 

 

 

 

 

Sato and Sato, 2011, Science

117 67 

S 

 

1,2 1 

1 

2 JST 

S 

 

1,2 

1 

2 JST, CREST 

6 

 

 

 

 

 

G 

 

Nature Cell Biol 2006; EMBO J 2003 

 

ArfGEF2/Big2 

 

 

Rab G 

 

Rab5 Rab11 

Rab7 

 

Neuron 2010 

 

 

 

 

 

 

 

 

 

3 

 

 

 

 

S 

 

 

 

 

 

FGF10 

 

 

 

 

 

FGF10 

 

FGF FGF 

2 

 

Phase Field 

in silico 

 

 

FGF 

 

 

S 

 

 

 

13.5 E13.5 

1 

E15.5 

E16.5 

 

 

 

10 

FGF10 

FGF10 

 

FGF10 

E13.5 E15.5 

 

FGF10 

 

 

FGF10 

FGF10 

FGF10 

FGF10

68 

117 

S 

ex vivo 

 

 

S 

 

 

 

 

 

 

 

 

 

 

 

 

ex vivo 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

13 

1 2 

 

 

 

A5G77f 

 

 

 

 

 

0.3 μm 

 

 

 

 

 

S 

 

1,2 

1 

2 

 

 

 

 

 

 

 

 

 

 

 

Nature Methods , 227 

230, 2007 

 

 

PNAS , 1347513480, 2009 

 

PLoS ONE , e21531, 2011 

 

 

 

S 

 

 

 

Tight junctions prevent the leakage of solutes through the paracellular 

pathway of epithelial cells and are indispensable in establishing the 

various compositionally distinct fluid compartments within the body 

of multicellular organisms. 

Based on morphological observations, two models about the tight 

junction have been proposed. The protein model proposes that a 

linear array of membrane protein particles polymerize to form a tight 

junction strand and that a network of these strands adhere with paired 

strands from apposing cells to obliterate the intercellular space. On 

the other hand, the lipid model, which originates from the observation 

of exoplasmic lipid leaflet fusion, proposes that the fusion of these 

lipid leaflets is associated with a transformation of the lamellar phase 

of the lipid bilayers into a non-lamellar, inverted micelle structure at 

tight junctions. 

Much has been learned about the membrane proteins at tight junctions 

over the last decade. However, how these membrane proteins function 

as the barriers in the paracellular space remains to be elucidated. 

Here, I will present recent progress toward a better understanding of 

supra-molecular complex at tight junctions.

117 69 

S 

The role of autophagyrelated proteins on lipid 

metabolism in the ER membrane 

Taki Nishimura 1 , Anoop Kumar Velikkakath 1 , 

Naotada Ishihara 1,2 , Eiko Oita 1 , Noboru Mizushima 1 

1 

Dept. Physiol. Cell Biol., Tokyo Med. Dent. Univ., 2 Dept. Protein 

Biochem., Inst. Life Sci., Kurume Univ. 

Autophagy is an intracellular degradation system accompanied by 

dynamic membrane organization. Dynamic membrane remodeling is 

achieved by the interplay between lipids and proteins and modulated 

by changes in lipid composition. However, the role of autophagyrelated 

genes in the lipid metabolism remains elusive. Here we 

show that endogenous Atg2A localizes on the autophagic isolation 

membranes and LDs. Atg2A and Atg2B are essential for autophagy 

because autophagic flux is blocked in cells treated with siRNA against 

both Atg2A and Atg2B, although LC3-II is generated. Morphological 

and biochemical analyses of Atg2-depleted cells reveal accumulation 

of aberrant membrane structures, which contained other Atg proteins 

such as LC3 and Atg9. These structures may represent intermediate 

structures of autophagosome formation. In addition, LD turnover 

is also affected in Atg2-depleted cells. These data suggest that 

mammalian Atg2 homologues are required for autophagosome 

formation and have an additional role in LD turnover, both of which 

take place on the endoplasmic reticulum. Now we are analyzing the 

effect of other autophagy-related genes RNAi on the LDs turnover. 

S 

ApoB 

 

 

 

 

 

Huh7 

ApoB 

ApoBcrescent ApoBcrescent 

 

ApoB 

ApoB 

Huh7 

UBXD2UBXD8p97 

UBXD2 UBXD8 p97 

UBXD8 ApoB 

UBXD8 knockdown ApoBcrescent 

ApoB UBXD8/p97 

UBXD8 

Derlin1 

ApoBcrescent 

Derlin1 knockdown UBXD8 ApoB 

 

ApoB Derlin1/UBXD8/p97 

 

 

S 

 

DGK 

 

 

DG 

 

DG C 

DG 

DG 

DG 

DG DGK DG 

 

DG 

DGK 

DGKDGKζ 

 

 

DGKζ 

 

DGKζ NMDA 

 

DGKζ 

 

DGKζ 

p53 

 

S 

 

 

 

Long chain fatty acids are important nutrients for brain development 

and function. However, the molecular basis of their actions in 

the brain is still unkown. Fatty acid-binding proteins (FABPs), 

intracellular chaperons of fatty acids, are involved in the promotion 

of cellular uptake and transport of fatty acids, the targeting of fatty 

acids to specific metabolic pathways, and the regulation of gene 

expression. We have so far revealed that FABP7, a strong binder 

of omega-3 PUFA, is involved in controlling fatty acid metabolism 

in the brain astrocytes and that its deficiency in mice results in the 

alteration of emotional behavioral responses. In this talk, I would 

introduce the possible mechanism by which FABP7 controls neuronal 

plasticity through regulating lipid metabolism in the astrocytes, and 

discuss the link of FABP- and/or PUFA-deficiency to the human 

psychosis including schizophrenia.

70 

117 

S 

 

1,2,3 1,2 1,2 1 

1 

2 3 CREST 

 

2 

 

 

1 5 

 

 

 

 

 

 

 

 

 

 

S 

 

 

 

 

 

 

 

 

P2X4 

ATP 

 

 

Nature 2003Nature 2005 

 

 

 

 

 

IRF8 IRF8 ATP 

 

 

IRF8 

IRF8 

 

S 

Ca + 

 

1 1 2 1 

2 2 1 

1 

BSI 2 BSI 

 

 

 

Ca 2+ 

Henneberger 2010 NatureAgulhon 

2010 Science 

in vivo 

 

Ca 2+ 2 

 

 

Ca 2+ Ca 2+ 

IP 3 R2KO 

 

WT DSer 

DSer NMDA 

KO DSer 

 

DSer 

 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Slit1

117 71 

S 

 

 

 

 

 

- 

 

ATP 

 

 

ionomycin 

ATP SNARE 

HATPase Ca 2+ 

ATP 

VNUT ATP MANTATP 

ATP 

ATP VNUT RNA 

KDATP 

ATP 

VNUTKD 

 

 

- 

 

S 

 

 

 

 

AME shh 

 

I 

 

II 

III 

shh 

AME 

AME 

 

AME 

 

AME 

AME 

AME 

shh 

AME Gli3 

shh K.O. GnRH 

LHβ 

 

AME 

S 

 

 

 

5 

FS 

 

 

 

 

 

 

ECMECM 

 

 

 

 

 

ECM 

living ECM 

ECM FS 

 

ECM 

FS 

ECM 

 

S 

 

 

1 2 3 2 

1 

1 

2 

3 

 

 

 

LH/FSH 

 

 

GnRH Leuprorelin 

LH/FSH 

 

 

 

GnRH 

 

 

Leuprorelin 

LH/FSH 

 

 

LH/FSH leuprorelin

72 

117 

S 

 

 

1 2 

1 

2 

 

 

 

 

 

 

 

5 

 

GH 

 

 

GH 

mRNA GH GHRH 

mRNA GHRH 

GH 

GHRH 

GH 

 

GHRH 

 

S 

 

 

 

 

3D 3DTV 

2D 

 

2D 

 

2 

 

 

 

 

 

 

 

3DTV 

 

S 

 

 

 

3 

2007 

3 

 

3D 

3 

 

3D 

 

 

3 

 

 

3 

3 

2 

3 

 

 

 

 

 

S 

 

 

 

3D 

 

2D 

3D 

3D 2D 

 

 

3D 

 

3D 

 

 

3D 3D 

 

 

 

3D 

 

3D 

3D 

3D

117 73 

S 

 

 

 

3D 

2 

 

 

 

3D 

3D 

 

 

 

 

 

 

3D 

TV 

 

 

 

3D 

 

3D 

 

S 

 

 

 

 

X 

AFM 

 

STORMDSLM 

X CTMRI 

 

 

 

 

 

 

 

3D 

3D 3D 

 

 

 

 

 

 

S 

 

 

 

VEGF 

Flk1 VEGFR2Flt1 VEGFR1 

ES Flk1 

 

 

 

 

Flk1 Flk1Cre BAC 

 

8 

 

Flk1 

Flk1 

 

 

Flk1 

 

180 

 

 

S 

 

 

 

 

 

 

 

 

 

Tie1:H2B::EYFP 

EYFP 

Sato et al., PloS One, 2010 

 

 

 

 

Tie1:H2B::YFP 

 

2

74 

117 

S 

 

 

 

 

100 Thoma 

 

Evans 

 

 

 

 

 

 

 

 

Danio rerio 

 

 

 

 

 

 

PICA 

 

 

S 

 

Brant Weinstein 

National Institutes of Health, NICHD 

The cranial vasculature is essential for the survival and development 

of the central nervous system, and is important in brain pathologies. 

Cranial vessels form in a reproducible and evolutionarily conserved 

manner, but the formation process remains unclear. We are using 

zebrafish to better understand the mechanisms underlying hindbrain 

vascular development by (i) carrying out a precise anatomical 

description of the formation process, (ii) investigating the molecular 

mechanisms guiding vascular patterning in the brain, and (iii) using 

genetic screens to identify new genes important for brain vessel 

formation. We found that the basilar artery is formed by a novel 

process of medial sprouting and migration of endothelial cells from a 

bilateral pair of veins, the primordial hindbrain channels. Subsequent 

second wave of dorsal sprouting from the primordial hindbrain 

channels give rise to angiogenic central arteries that penetrate into 

the hindbrain. We have also discovered that chemokine signaling 

is required to direct basilar artery assembly. I will report on these 

and other novel molecular processes involved in the assembly of 

hindbrain vascular networks. 

S 

Coordinated branching morphogenesis of coronary 

vessels and peripheral sympathetic nerves in the 

developing heart 

Yosuke Mukoyama 1,2 

1 

National Institutes of Health, 2 National Heart, Lung, and Blood 

Institute 

Anatomical proximity and close patterning of peripheral nerves 

and blood vessels suggest that there is interdependence between 

the two networks. The first such indication of this interplay is the 

responsiveness of vascular development to signals secreted by 

peripheral sensory nerves in the developing skin. Interestingly, we 

discovered a reciprocal guidance event in the patterning of peripheral 

sympathetic nerves in the developing heart. Our whole-mount 

imaging approaches revealed that sympathetic axons branch alongside 

large-diameter coronary veins in the surface layer of the dorsal 

ventricular wall prior to innervating final targets such as coronary 

arteries and cardiomyocytes in the deeper myocardial layer. Further 

genetic studies and in vitro organ culture experiments demonstrated 

that coronary veins serve as an intermediate template that guides 

distal sympathetic axon projection via local secretion of NGF by 

coronary vascular smooth muscle cells. Our results suggest that target 

organs possess unique and stereotypical patterns of innervation, 

mediated by tissue sub-structures, such as coronary veins in the heart, 

which adapt to complex organ structure and physiology. 

S 

CT 

 

 

CT 

 

 

23 4 

AI CT 

 

CT 

 

 

 

 

 

 

 

 

 

 

CT 

3 

 

CT

117 75 

S 

 

 

1 2 1 1 1 

2 2 

1 

2 

22 7 

 

11 

16 multidetector CT DSA 

PACS 

1 5 

5 8 12 4 

FA2 1 

2 FA 

CT 

 

FA 1 

2 

FA 

 

FA 

 

 

CT 

CT 

CT 

 

S 

CT 

 

1,2 

1 

2 

 

 

 

 

CTComputed Tomography 

 

CT 

 

 

 

CT 

CT 

CT 

 

CT 

 

CT 

 

 

CT 

 

S 

 

 

 

 

2022 

 

 

AIES 

 

 

 

LAN 

 

CTMRI AIES 

 

CTMRI 

 

CT 

OsiriX 3 

CTMRI 

WordPowerpoint 

PDF PC iPad 

 

 

S 

CT 

1 1 1 2 2,5 

3 4 1 

1 

2 

3 4 5 

 

 

A CT 

21 23 

 

 

CT CT 

CT 

 

 

 

21 

2223 OsiriX iPod touch 

23 iPad

76 

117 

S 

Underlying mechanism that generates a phase wave 

in the mammalian circadian center 

1 1 1 1 2 

1 

2 

S 

 

 

1,2 

1 

2 

The suprachiasmatic nucleus (SCN) is the center of the mammalian 

circadian rhythm. Individual oscillating neurons have diverse 

endogenous circadian periods but usually they are synchronized by 

intercellular coupling mechanism. In the present study, we artificially 

disrupted the intercellular coupling among oscillating neurons in 

the SCN and observed the regional differences of the period of the 

oscillating small-latticed regions on the SCN by using a transgenic 

rat bearing a luciferase reporter gene driven by regulatory elements 

from a per2 clock gene (Per2::dluc rat). The analysis divided the 

SCN into two regions; one region showing periods less than 24h (SPR) 

and another region showing periods longer than 24 hours (LPR). 

The SPR lies in the medial smaller region of the dorsal SCN and the 

LPR occupied the remaining larger region. Interestingly, the SPR 

corresponds well with the region in which the phase wave in the SCN 

is launched. Further, we introduced a mathematical model in order to 

investigate the kinetics of the oscillators and the function of the short 

period region in the SCN. 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

in vivo Multiunit nueral 

activityin vivo MUA 

 

Clock MUA 

- 

 

S 

 

 

 

 

1 

 

 

- 

 

1997 

 

 

 

 

 

 

 

 

1988 

 

 

 

 

 

S 

 

 

1,2 

1 

2 JST, 

We spend almost one third of our life time just to sleep. Sleep/ 

wakefulness cycle is a very intriguing physiological phenomenon. 

However, the mechanism regulating sleep/wakefulness cycle has 

not been completely understood so far. Although orexin neurons in 

the hypothalamus have a crucial role in the regulation of sleep and 

wakefulness, how orexin neuronal activity promotes wakefulness 

is incompletely understood. To further examine the role of orexin 

neuronal activity in sleep/wakefulness regulation, we generated 

transgenic mice in which orexin neurons expressed halorhodopsin, 

an orange light-activated chloride ion pump. Slice patch clamp 

recordings of orexin neurons demonstrated that photic illumination 

hyperpolarized and reduced the discharge rate of orexin neurons 

in a wavelength- and intensity-dependent manner. Acute silencing 

of orexin neurons in vivo by orange light illumination decreased 

electromyography power and increased the delta frequency in the 

electroencephalogram, indicative of slow wave sleep, and was timeof-day 

dependent. These findings suggest that activation of orexin 

neurons is necessary to keep animals awake during the light period.

117 77 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

GSK3β 

glycogen synthase kinase 3β 

GSK3β 

REVERBαBmaL1 

 

 

Polg DNA 

 

 

 

 

 

S 

 

 

 

II 

 

 

 

 

 

 

 

 

 

19 

1950 

 

1980 

cytochrome C oxidase 

targeting signal 

 

 

 

 

21 

 

 

S 

 

 

1,2 1 2 

1 

2 

 

 

 

 

 

 

 

 

 

Cyanidioschyzon merolae 

12 μm 

 

100% 

 

MD/PD 

ER 

 

 

VIG1 

 

 

 

 

S 

 

 

 

 

 

mtDNA 

mtDNA 

 

HG D Lancet 1998HG 

D4a Hum Genet 2007; PLoS One 

2009mtDNA 

HG N9a 2 Am J Hum Genet 2007 

Diabetes 2007 

HG N9b 

Hum Genet 2007HG A 

Mitochondrion 2007 

HG A HG M7a 

J Atheroscler Thromb 2010 

HG G1 

HG F Br J Sports 

Med 2010mtDNA

78 

117 

S 

β 

1,2 

1 

II 2 

 

ATP 

 

 

CoA TCA ATP 

β 

CoA 2 

β 

20 1950-70 

70 

β 90 

β 

2 β 

 

2 β 2 

 

ATP 

 

β 

 

β 

 

S 

 

 

 

TCA β 

 

β 

 

 

GC/MS 

ESIMS/MS 

 

 

 

 

β 

in vitro probe assay β 

 

 

 

β 

β 

β 

 

 

 

S 

 

 

 

II 

β 

 

β 

 

 

PAG 

DNA 

 

β Leydig 

 

Sertoli 

 

 

β 

1/10 

 

Sertoli β 

 

Sertoli 

Sertoli 

 

Fukasawa M et al. J Histochem Cytochem 58: 195–206, 

2010 

S 

 

 

 

II 

 

glucoseonly dogma 

β 

dogma 

 

Müller 

PAG 

 

DNA 

 

β Müller 

 

β 

 

1/10 Müller 1/2 

4 Müller 3 

β 

Müller 

 

β 

 

Atsuzawa K et al. Histochem Cell Biol 134: 565–579, 

2010

117 79 

S 

 

 

 

 

 

niche 

DNA 

 

endosteal niche 

 

 

Angiopoietin1 Thrombopoietin 

 

 

ATM 

ROS p38MAPK 

 

ROS 

 

 

HIF1α VHL 

 

 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

GFAP 

GFAPGFP GFAPCre 

 

 

GFAP 

GFAP / 

 

S 

 

 

 

 

 

 

 

MSCs 

 

 

MSCs 

 

MSCs 

 

MSCs 

hMSCs 

PACAP 

hMSCs 10 

PACAP 

PACAP 

hMSCs 

PACAP hMSCs 

PACAP hMSCs 

 

S 

 

 

2 

 

 

 

 

 

 

 

GFP 

GFP 

C57BL/6 5 

 

 

mRNA CCR2 high / 

CX 3 CR1 low /CXCR4 high CCR2 

/ CX 3 CR1 high /CXCR4 low 

MCP1 SDF1 

GFP + /CXCR4 high β 3

80 

S 

 

 

2 

117 

S 

 

 

 

 

 

 

 

 

 

proinsulin TNFα 

 

 

 

 

In vivo 

in vitro 

 

Y Y 

TNFα 

 

 

 

 

 

 

 

 

 

 

 

 

polycomb group repressor complex PcG 

 

 

 

 

S 

 

 

 

bHLH Hes1 

Hes1 

Neurogenin2 Ngn2 

Ngn2 

Ngn2 

 

 

Ngn2 

Ngn2 

Ngn2 

Ngn2 

Ngn2 

Ngn2 

Ngn2 

 

Ngn2 

 

 

S 

 

 

 

6 

insideout 

 

 

 

 

 

 

4536233 

 

 

 

 

 

 

 

 

insideout

117 81 

S 

GABA 

 

 

GABA 

Cl KCC2ClNKCC1Cl 

NKCC1 Cl [Cl]i 

 

 

 

 

GABA 

GABA 

 

GABA 

 

GABAa Ca2+ 

 

GABA NKCC1 [Cl]i 

GABA 

KCC2 GABA 

 

Cl GABA 

 

GABA 

GABAa 

 

S 

Ai 

 

1 2 2 1 1 

2 2 1 

1 

2 

 

1 23 6 

Ai 

X CT Autopsy Imaging 

Ai 

3 

3D 

 

ipad 

 

 

2 

 

 

 

 

 

 

OB 

 

 

 

S 

 

 

 

 

1985 4 

 

 

 

 

 

5 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

S

82 

117 

S 

 

 

 

12 

 

 

1 

 

 

 

2 72 

20 

 

6 

 

6 

 

 

 

 

 

 

 

 

 

 

 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

S 

 

 

 

 

Mes5 

 

 

Mes5 

brain 

derived neurotrophic factor BDNF 

dystonin Brn3a Mes5 

 

3 

Mes5 

BDNF Mes5 

 

dystonin 

Mes5 wild 

type Brn3a 

 

 

Brn3a 

Mes5 

 

BDNF Brn3a 

S

117 83 

S 

 

 

 

By means of retrograde transneuronal transport of rabies virus, 

ascending multisynaptic pathways from the trigeminal ganglion (TG) 

to the anterior cingulate cortex (ACC) were identified in the rat. After 

rabies injection into an electrophysiologically-defined trigeminal 

projection region of the ACC, transsynaptic labeling of secondorder 

neurons via the medial thalamus (including the parafascicular 

nucleus) was located in the spinal trigeminal nucleus pars caudalis. 

Third-order neuron labeling occurred in the TG. Most of these TG 

neurons were medium-sized or large cells giving rise to myelinated 

Aδ or Aβ afferent fibers, respectively. Furthermore, the TG neurons 

retrogradely labeled with fluorogold injected into the mental nerve 

were smaller in their sizes compared to those labeled with rabies. Our 

extracellular unit recordings revealed that a majority of ACC neurons 

responded to trigeminal nerve stimulation with latencies of shorter 

than 20 ms. Thus, somatosensory information conveyed to the ACC 

by multisynaptic ascending pathways derived predominantly from 

myelinated primary afferents and may be used to subserve affectivemotivational 

aspects of pain. 

S 

 

 

 

 

TSNC 

Vmo 

 

FG 

BDA 

 

 

TSNC S1 

 

 

Vmo 

Vmo 

Vmo 

Vmo 

 

 

S1 

 

 

 

S 

 

 

 

 

 

 

 

 

Vm 

 

1 

ACeRFp 

Vm ACe Vm 

RFp 2 

ACe RRF RFp 

GABA ACe RFp 

RRF 3 

PLH RFp Vm 

PLH Vm 

RFp 

ACe PLH PLH GABA 

 

 

 

S 

ES 

1 2 2 2 2 

1,2 

1 

 

2 

 

MSCs 

 

 

 

 

MSCs ES 

MSCs 

 

Differentiation 2011ES MSCs 

 

ES MSCs 

 

 

24 ES MSCs 

 

CatWalkNoldus 

ES MSCs 

MSCs 

 

 

1

84 

117 

S 

BFI FIB/SEM 

 

1 2 2 1 

1 1 1 

1 

2 

 

/ 

 

iPS 

Muse PDGFRα 

 

SEM BFI 

FIB/SEM 

BFI 

SEM 

 

 

FIB/SEM SEM 

FIB BFI 

 

 

 

 

3 

 

 

S 

 

1 2 1 2 1 

1 

2 2 1 

Mesenchymal stem cell, MSC 

 

MSC DSS 

 

MSC 

MSC 

MSC 

conditioned medium, MSCCM 

DSS 

IEC6 

MALDITOFMS MSCCM 

MSCCM DSS 

IEC6 PI3KAkt 

 

 

T MSCCM 

VEGF, IL13, TIMP 

 

MSC gut trophic factor 

 

S 

Motile and immune functions of intestinal 

subepithelial myofibroblasts 

Islam Md Shafiqul, Hiroshi Ozaki 

Department of Veterinary Pharmacology, Graduate School of 

Agriculture and Life Sciences, The university of Tokyo 

Intestinal subepithelial myofibroblasts (ISMF) are a syncytium of 

α-SMA-positive, mesenchymal cells, which reside subjacent to the 

basement membrane of the small and large intestines. ISMFs are 

considered to mediate the movement of intestinal villi, regulation of 

epithelial cell proliferation and differentiation, and mucosal protection 

under physiological condition. ISMFs secrete many proinflammatory 

cytokines, growth factors, chemokines and other inflammatory 

mediators, as well as extracellular matrix proteins. This allows ISMF 

cells to act in a paracrine and autocrine fashion to mediate immune 

functions under pathological conditions. Extracellular matrices, such 

as collagen, fibronectins and tenascin C (TnC), secreted by ISMFs 

are important in tissue remodeling and tissue repair. Our laboratory 

findings have been demonstrated that TnC and α-SMA densely 

expressed in DSS-induced colitis model mice particularly at the sites 

of inflammation, as well as in SAMP1/Yit spontaneous ileitis model 

mice. We have also identified ISMF as one of the TnC producing 

cells in the intestine. In this symposium, I shall describe the recent 

development of ISMF research, especially focusing TnC. 

S 

 

 

 

 

 

 

fibroblastlike 

cells PDGF α SK3 

 

 

 

 

CD34 1 

 

 

ATP NO 

 

 

 

PDGF 

α CD34 PDGF α 

NG2 

ATP P

117 85 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

S 

PBL 

 

 

 

 

 

 

 

 

 

 

PBL 

14 10 400 

120 1 

PBL 

 

Cadaver Seminar 

 

S 

 

 

 

 

23 

 

1 

30 

 

55 35 

 

 

 

 

 

 

6 

6 3 

 

 

 

 

 

 

S 

 

 

 

2 

 

2004 

 

3 

 

 

CT MRI 

 

 

 

 

 

 

7080 34

86 

117 

S 

 

 

 

 

 

 

 

2 

4 10 3 

 

 

 

 

 

 

 

 

 

S 

 

nonIT 

 

 

 

 

 

 

 

 

 

 

 

 

 

15 WebCT 

2 

 

 

 

 

 

30 

IT 

 

 

S 

 

 

 

 

 

 

 

 

600 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Arp2/3

117 87 

S 

 

 

1,2,3 

1 

 

2 

CREST, JST 3 SENTAN, JST 

 

 

 

 

 

3 

 

 

 

SN 

 

 

 

 

 

Eos 

 

 

 

 

Eos 

 

S 

 

1,2 2 

1 

2 

 

 

 

 

 

20nm 

 

 

 

 

 

II 

 

WASP, VASP, IQGAP1 

 

 

 

 

 

 

 

 

S 

CEMOVIS 

 

1,2 1,2 1,3 

1 

2 CREST,JST 3 

 

 

 

 

 

 

CEMOVISCryoElectron Microscopy of Vitreous 

Sections 

 

 

 

 

 

 

 

 

 

 

CEMOVIS 

 

 

 

S 

 

 

 

 

SDS SDSFRL 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

SDSFRL

88 

117 

S 

 

 

 

 

 

 

 

 

G1 

DNA S 

G2 

 

 

 

 

 

 

G2 

 

G1 

 

 

 

 

 

S 

 

 

 

 

Microtubule (MT) organization plays a key role in neuronal migration 

and axon/dendrite formation. Here we monitored the dynamics of 

centrosome and MT plus ends in neocortical neurons. In multipolar 

migrating neurons, newly emerging processes formed irrespective 

of the centrosome localization. The centrosome tended to move 

toward the dominant growing process. In locomoting neurons, which 

have a bipolar shape, the centrosome was targeted toward the piadirected 

leading process. However, the centrosome at the base of the 

leading process, in front of the nucleus, was occasionally overtaken 

by the translocating nucleus. When a locomoting neuron formed an 

axon-like trailing process opposite the direction of migration, the 

centrosome was located on the other side of the cell. MT growth in 

the leading processes of bipolar neurons was mostly directed toward 

the distal tip, while few bidirectional MT plus-end movements were 

detected in the trailing process. Depletion of gamma-tubulin in these 

neurons resulted in stacking of migration-defected neurons in the 

intermediate zone. Interestingly, neurons that managed to migrate into 

the cortical plate showed striking abnormalities in orientation. 

S 

 

 

1,2,3 

1 

 

2 

3 

 

 

 

6 

 

 

 

 

 

 

 

 

S 

stathmin 

1 1,2,3 4 4 5 

2 1,2,3 

1 

 

2 3 

4 

5 

 

 

 

PACAP 

 

PACAP 

PACAP KO PACAP 

 

α/β tubulin 

stathmin1 Stathmin1 

subgranular zone PACAP KO 

stathmin1 axon 

stathmin1 axon 

stathmin1 

axon 

 

PACAP stathmin1 axon 

abnormal arborization 

 

stathmin1 adult neurogenesis 

stathmin1

117 89 

S 

C 

1 1 2 

1 

2 

 

1 

 

 

DA 5HT 

5HT 2C 5HT 2C R 

5HT 2C R 

5HT 2C R 

5HT 2C R 2 5AE 

RNA 

 

2 

 

C57BL/6J RNA 

VXV 1.5 

C3H/HeJ RNA 

5HT 2C R RNA 

ADAR1/2 

5HT 2C R C57BL/6J 

 

5HT 2C R RNA 

 

S 

 

1,2 1 1 

1 

2 

 

G GPCR 

 

GPCR 

 

 

 

 

GALP WNPW 

 

GALP NPW 

 

 

GALP NPW 

 

 

GALP 

 

GALP 

NPW 

 

 

S 

kisspeptin 

 

 

 

puberty 

 

 

 

 

GPR54 kisspeptin 

kisspeptin GnRH 

 

HPG 

axis kisspeptin kisspeptinHPG axis 

 

 

 

 

 

Kisspeptin 

 

 

 

Kisspeptin 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

POMC

90 

117 

S 

 

 

 

 

 

 

 

 

 

 

 

ER 

ER 

 

 

ERERαERβ 

 

ER 

2 ER 

 

 

 

 

 

S 

 

 

Fumiyo Aoyama, Nobuyasu Takahashi, Soyuki Ide, 

Akira Sawaguchi 

 

 

 

2 

 

 

 

 

 

 

 

 

 

 

 

 

 

H2 

 

 

 

 

 

S 

 

 

1,2 1 1 

1 

2 

IVCT 

in situ IVCT 

1 

a BSA 

IVCT 

 

T BSA IgG 

b IVCT 

IgG 

 

2 IVCT 

focal adhesion kinase FAK 

FAK 

3 3 

horseradish peroxidase 

/ 

IVCT 

IVCT 

 

 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

FIBSEM 

 

 

Palade

117 91 

S 

Focused Ion BeamScanning Electron Microscopy 

FIBSEM Live imaging 

 

1,2 

1 

 

2 

Department of Neurosciences, Lerner Research Institute, Cleveland 

Clinic 

 

 

 

 

FIBSEM 

 

Na+ 

Ranvier NR Na+ 

NR 

 

NR Internode NR 

Slice 

Live imaging Na+K+ ATPase Internode 

NR 

 

FIBSEM Live imaging 

 

 

 

 

 

 

S 

CEACAM 

 

 

 

mRNA Differential Display 

5 Ceacam6 

Ceacam6 isoform, Ceacam6L 

 

CEACAM6L 

 

apical Ectoplasmic Specialization 

 

CEACAM6L CEACAM6L 

 

CEACAM6L 

 

 

CEACAM CEACAM2 

 

 

 

S 

 

 

 

 

3 

 

 

 

 

 

Cell adhesion molecule1 Cadm1 

Poliovirus receptor Pvr 

Nectin3 Nectin2 

 

 

 

 

 

 

S 

 

 

 

 

 

 

 

immune priviliged organ

92 

117 

S 

Implications of histone modification and DNA 

methylation during mouse spermatogenesis 

Ning Song, Takehiko Koji 

Department of Histology and Cell Biology, Nagasaki University 

Graduate School of Biomedical Sciences 

Epigenetic factors such as histone modification and DNA methylation 

have been implicated in the regulation of gene expression. However, 

the association of those factors with a specific stage of germ cells 

during spermatogenesis has not been fully understood. In the present 

study, we analysed DNA methylation states quantitatively in each 

germ cell by a novel method, HELMET. Histone H3 modifications 

were examined by immunohistochemistry. The methylation ratio 

of CCGG sequence gradually increased to a maximum in the 

stage from pachytene spermatocytes to round spermatids, and then 

declined. The increase in DNA methylation seemed to be correlated 

with deacetylation of histone H3, indicating genes are generally 

inactivated transcriptionally. We also found the promotion of histone 

H3 acetylation in parallel with DNA demethylation in elongating 

spermatids, may facilitate histone-protamine exchange. Interestingly, 

in vivo treatment of mice with 5-azadC reduced DNA methylation, 

but also induced H3K4me3 in spermatogonia accompanying with 

frequent apoptosis. These results indicate coordinate changes in the 

modifications of DNA and histone, and their close association with 

spermatogenesis. 

S 

Unique integration of fertilizationrelated protein 

Equatorin EQT into the acrosomal membrane 

during spermatogenesis 

Chizuru Ito, Kenji Yamatoya, Kiyotaka Toshimori 

Dept. Anatomy and Developmental Biology, Grad. Sch. Med., Univ. 

Chiba 

EQT is a widely distributed fertilization-related acrosomal protein in 

mammalian sperm. During the acrosome reaction some quantity of 

EQT is translocated on the plasma membrane covering the equatorial 

segment (ES) where sperm fuses with an oolemma, while the 

majority remains on the inner acrosomal membrane (IAM) until male 

pronucleus is formed. To analyze the nature of the EQT it is important 

to investigate its origin and expression during spermatogenesis. In 

this study we show the integration process of EQT in wild and EQT- 

EGFP transgenic mice using immunoelectron microscopy and highresolution 

light microscopy including STED nanoscopy. EQT mRNA 

was first detected in stage I-VII pachytene spermatocytes and then 

in step1-7 round spermatids. EQT protein was initially detected on 

the nascent outer acrosomal membrane of step 2 round spermatids 

and then gradually accumulated mainly on the IAM of the ES in 

elongating spermatids. EQT was not found acrosomal granules. We 

also found EQT forms complex with acrosomal matrices and the 

perinuclear theca (PT) by MS/MS analysis after immunoprecipitation. 

This evidence suggests EQT co-translocates associating with 

acrosomal matrices and PT. 

S 

 

 

 

 

 

 

 

 

 

 

3 4 

 

 

 

 

 

5 3 

Hoxa11/d11/d12 

 

Hox 

 

 

 

 

 

 

 

S 

 

 

 

 

1996

117 93 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2 

 

 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

S 

 

 

 

1 3 

 

 

 

 

 

 

 

 

 

 

19 

2 

 

 

 

 

 

 

 

S 

 

1 2 

1 

2 

 

 

 

Sabin 1902 

 

Huntington 1908 

Prox1 

 

 

Sabin 

 

 

 

 

Isogai 2010

94 

117 

S 

MRI 

MRTD 

1,2 2 1 3 4 

4 

1 

2 

3 4 

 

 

 

 

 

 

 

99mTcphytat 

 

 

 

 

 

MRI MRM 

MRCP 

Magnetic 

ResonanceThoracic Ductography MRTD 

 

 

 

MRTD 

 

 

 

S 

Aspp 

 

 

 

p53 Aspp1 

Aspp1 

Aspp1 / Aspp1 / 

 

 

 

Aspp1 / 

 

 

 

Aspp1 / 

 

 

 

Aspp1 / 

 

Aspp1 

 

S 

Virchow 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

29 

 

 

 

 

S 

 

 

1,4 2 3 1 

Brant Weinstein 4 

1 

2 

3 

4 Section on Vertebrate 

Organogenesis, NICHD, NIH 

 

 

 

 

 

 

 

 

 

 

shear stress 

 

 

 

 

 

 

Huntington 

Kampmeier

117 95 

S 

 

 

 

 

 

 

 

 

 

 

 

 

S 

 

 

 

 

 

 

 

 

 

 

10 

 

 

aPKCPAR 

 

 

 

 

 

 

1 

2 

 

 

 

 

S 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

S 

 

 

 

axon initial segment, AIS Na 

 

AIS 

 

 

 

 

nucleus magnocellularis, NMAIS 

 

NM 

NM 

AIS 

NM 

 

NM AIS 

1.5 

NM 

 

AIS

96 

117 

S 

 

1,2 1,2 1 1,3 

4 - 1 1,5 1 

1 1,6 1 

1 

2 

3 

JST, CREST 4 5 

6 MRC 

 

Corticothalamic projection neurons (CTNs) in the cerebral cortex 

constitute an important component of the thalamocortical reciprocal 

circuit, an essential input/output organization for cortical information 

processing. However, the spatial organization of local excitatory 

connections to CTNs is only partially understood. Here, applying a 

newly developed adenoviral vector, we retrogradely visualized almost 

all layer (L) 6 CTNs from their cell bodies to fine dendritic spines in 

the rat barrel cortex. In cortical slices containing visualized L6 CTNs, 

we intracellularly stained single L2/3, L4, L5, and L6 pyramidal/ 

spiny neurons and morphologically examined their local connections 

to CTNs. The CTNs received strong and focused connections from 

the L4 neurons just above them, and the most numerous nearby and 

distant sources of local excitatory connections to CTNs were CTNs 

themselves and L6 putative corticocortical neurons, respectively. The 

present results suggest that, through CTNs, L4 neurons together with 

L6 neurons may serve to modulate thalamic activity. 

S 

 

Recurrent connection selectivity of layer V pyramidal 

cells in frontal cortex 

1,2 1,2 

1 

2 

Pyramidal cells in the neocortex are differentiated into several 

subgroups based on their extracortical projection targets. However, 

little is known regarding the relative intracortical connectivity 

of pyramidal cells specialized for their targets. We used paired 

recordings and quantitative morphological analysis to reveal 

distinct synaptic transmission properties, connection patterns, and 

morphological differentiation of rat frontal cortex. Retrograde 

tracers were used to label two projection subtypes in L5: crossedcorticostriatal 

(CCS) cells projecting to both sides of the striatum, 

and corticopontine (CPn) cells projecting to the ipsilateral pons. 

Although CPn/CPn and CCS/CCS pairs had similar connection 

probabilities, CPn/CPn pairs exhibited greater reciprocal connectivity, 

stronger unitary synaptic transmission, and more facilitation of 

paired-pulse responses. These synaptic characteristics were strongly 

correlated to the projection subtypes. CPn and CCS cells were further 

differentiated in their dendritic/ axonal arborization. Together, our 

data demonstrate that the pyramidal projection system is segregated 

according to subcortical target. 

S 

A Hz Oscillation Synchronizes Prefrontal, VTA, and 

Hippocampal Activities during Working memory 

Gyorgy Buzsaki 

Rutgers University 

Network oscillations support transient communication across brain 

structures. We show here, in rats, that task-related neuronal activity 

in the medial prefrontal cortex (PFC), hippocampus and ventral 

tegmental area (VTA), regions critical for working memory, is 

coordinated by a 4-Hz oscillation. A prominent increase of power 

and coherence of the 4-Hz oscillation in the PFC and VTA and its 

phase-modulation of gamma power in both structures was present 

during working memory. Subsets of both the PFC and hippocampal 

neurons predicted the turn choices of the rat. The goal-predicting 

PFC pyramidal neurons were more strongly phase-locked to both 

4-Hz and hippocampal theta oscillations than non-predicting cells. 

The 4-Hz and theta oscillations were phase-coupled and jointly 

modulated both gamma waves and neuronal spikes in the PFC, VTA 

and hippocampus. Thus, multiplexed timing mechanisms in the PFC- 

VTA-hippocampus axis may support processing of information, 

including working memory. 

S 

Development of orientation and direction selectivity 

in the mouse visual cortex 

1,2 Nathalie Rochefort 2 Christine Grienberger 2 

Nima Marandi 2 Daniel Hill 2 Arthur Konnerth 2 

1 

2 Inst. Neuroscience, Technical 

Univ. Munich 

Functional features of the cortical neurons such as direction 

selectivity (DS) in the visual cortex are established during 

development. Previous studies of the ferret visual cortex implied 

that experience-dependent plasticity of local circuits in the cortex 

contributed to the development of DS. In spite of their usefulness, it 

has been less understood that how the rodent visual system develops. 

Here we used two-photon Ca 2+ imaging to study the development of 

DS in layer 2/3 neurons of the mouse visual cortex in vivo. At eyeopening, 

nearly all orientation-selective neurons were also directionselective. 

DS developed normally in dark-reared mice, indicating that 

the early development of DS is independent of vision. Furthermore, 

remarkable functional similarities existed between the development 

of DS in cortical neurons and the previously reported development 

of DS in the mouse retina. Together, these findings suggest a new 

experience-independent circuit mechanism for the development of DS 

in the mammalian brain. Since rodents lack columnar organization 

in the visual cortex, difference in the local connectivity may explain 

different developmental profile between species.

117 97 

W 

WG 

1,2 

1 

2 

 

 

 

WG 

 

 

 

 

 

 

MDPhD 

 

 

3 MD 

PhD 

 

 

 

 

 

 

 

 

W 

 

 

1 1,2 

1 

 

2 

 

 

 

 

 

 

4 

 

 

2006 

 

 

17 30 

 

 

4 

2010 2 

 

 

 

 

 

W 

 

 

1 2 

1 

2 

 

 

 

 

 

 

 

14 PhDMD 

20 MD 

23 

 

 

 

 

 

 

 

 

 

MD 

 

 

 

W 

 

1 2 2 3 

1 1 1 

1 

2 M2 3 M4 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1

98 

117 

W 

 

 

 

 

 

 

 

 

 

 

CBT 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

W 

 

 

 

 

23 MDPhD 

2-4 

3 

1 

 

 

 

19 

 

 

 

MDPhD 8

117 99 

OEPMI 

Singleneuron tracing study of thalamocortical projections arising 

from the rat mediodorsal nucleus 

 

 

The mediodorsal thalamic nucleus (MD) receives inputs from a wide variety of 

subcortical structures, such as the amygdala, basal forebrain and brain stem, and 

projects its axons to the prefrontal cortex. The MD have been considered to play 

important roles in learning, working memory, emotion, and behavioral control. 

In the present study, we investigated dendritic and axonal arborizations of single 

MD neurons in rats using Sindbis viral vectors expressing membrane-targeted 

green or monomeric red fluorescent protein. Single MD neurons infected with 

the viral vectors were completely visualized by immunoperoxidase staining for 

fluorescent protein, and reconstructed. When the axons exited from the thalamus, 

the reconstructed MD neurons always emitted axon collaterals to the thalamic 

reticular nucleus. Two of the 6 reconstructed MD neurons formed abundant axon 

bush in the neostriatum. In the cerebral cortex, the MD neurons sent axon fibers 

mainly to middle layers of the orbital, prelimbic, cingulate and frontal association 

areas. Thus, MD neurons would be classified into core thalamic neurons according 

to Jones (1998, 2001). 

OEPMI 

V 

Stefan Trifonov 

 

Calbindin D28K 

Calbindinpoor compartment 


 


GAD1 mRNA Cannabinoid type 1 receptor 

CB1 mRNA in situ hybridization 

1 Calbindinpoor 

compartment Anterior, Middle, Posterior 3 

2 GAD1 mRNA Middle region 3 CB1 

mRNA 3 4 Anterior region Posterior region 

 

Middle region V 

OEPMI 

 

 

 

 

D1 D2 D1RD2R 

 

D1R D2R in situ 

 

D1R 

mRNA D2R mRNA 

D1R D2R 

 

D2R 

 

 

 

 

D1R D2R 

 

 

 

 

OEPMI 

GABA 

 

 

 

PV GABA 

 

 

Correlated CLSMEM 

 

4 PV 

 

vGLuT2 

driving input 

4 PV PV 

4 PV 

2/3 

PV 

 

CA1 

 

 

 

OEPMII 

BAF dpf GFP 

 

 

 

BAF 10 

DNA 

BAF 

esBAFnpBAF 

nBAF ES 

 

 

zinc Dpf1 nBAF 

BAF45b 

Dpf1 

 

tTAEGFP 

dpf1 

dpf1tTAGFP 24 

GFP 48 

 

dpf1 GFP 

Dpf1 

Dpf1 

OEPMII 

mRNA 

1 2 2 1 

1 

2 

 

 

 

 

 

 

mRNA 

 

 

 

 

mRNA 

 

 

RNA 

 

RNA 

mRNA

100 

117 

OEPMII 

Immunocytochemical and proteomic analysis of the γsecretase 

complex using the conformationspecific antiNicastrin antibody 

1,2 2 4 3 4 5 

2 

1 

2 3 

4 5 

 

γSecretase is a membranespanning, multimeric protease that cleaves APP and 

is responsible for generating the Aβ peptides. Although the intracellular traffic is 

implicated in the regulation of the Aβ production, exact subcellular location where 

the enzyme functions remains controversial. Here we established a monoclonal 

antibody A5226A against Nicastrin, one of the γsecretase components, and found 

that A5226A specifically recognizes the active γsecretase. Immunocytochemistry 

using A5226A revealed that γsecretase localizes at the cell surface and late 

endosome. Moreover, we applied A5226A for the targeted proteomics of the 

γsecretase complex. We identified about 30 interacting proteins including 

tetraspanin family members such as CD81, which are known to form membrane 

microdomains. We found that knockdown of CD81 reduced the secretion of Aβ. 

Furthermore, the internalization rate of both the enzyme and APP was reduced 

by knockdown. These results suggest that CD81 affects the Aβ generation via 

regulating the traffic of the enzyme and the substrate from the cell surface. 

OFPMI 

Rho ROCK, ROCK 

1,2 2 2 1 1 

1 2 

1 

2 

GTP Rho 

Rho 

Rho Rho 

Rho 

Rho ROCK1, ROCK2 

 

Rho 

ROCK1 ROCK2 

ROCK1 

ROCK2 

ROCK1 

ROCK2 

ROCK2 

 

 

Rho 

 

OFPMI 

Photoactivatable Rac 

 

 

GTPase Rac1 

 

 

phototropin LOV2 GTP Rac1 photo 

activatable PARac1 

PARac1 

RAW Rac1 

photo 

manipulation 

mCherry PARac1 

 

 

 

 

 

 

 

Rac1 

 

OFPMI 

GaAsP LIVE 

 

1 2 1 1 3 

1 

1 

2 3 

 

 

 

 

 

A1, A2, A5LIVE 

GaAsP 

 

 

 

GaAsP 

GFP 

BSC1 

LIVE 

GaAsP 

A1, A2, A4, A5 A7 

 

 

OFPMI 

E 

CD 

 

1 

229EHCoV229E CD13 

 

 

HCoV229E 

CD13 

 

HCoV229E CD13 

CD13 

1 CD13 

2 

mCherryβactin 

EGFPlifeact CD13 Factin 

CD13 Factin 

cytochalasin Blatrunculin A actin 

CD13 Factin 

 

CD13 Factin 

OFPMII 

Nphp 

 

 

 

650 

 

Nphp3 Nphp3 1325 

coiledcoilCC 

Nphp3 

 

 

Nphp3GFP 

 

 

Nphp3 N 200 

N N 

CC 2 Nphp3 

Nphp3 

Kif7, Tctn2, Arl6 

Nphp3

117 101 

OFPMII 

 

1 2 3 2 3 2 

3 1 

1 

2 

3 

 

Cilia show variations in axonemal structure, motility, and the number per 

cell, and are classified into subtypes. Depending on the tissue origin and 

differentiation status, one cell type appears to adopt a certain ciliary subtype. We 

are characterizing unique, multiple 9+0 cilia in choroid plexus epithelial cells 

CPECs to elucidate the mechanism of ciliary diversification. Proteomic analysis 

of CPEC cilia identified 868 proteins, including several molecules implicated in 

ciliary motility such as Rsph9. Immunostaining for Rsph9 demonstrated that the 

molecule localized to a subpopulation of CPEC cilia. Live imaging of choroid 

plexus tissue exhibited that some CPEC cilia could beat vigorously at the neonatal 

stage, whereas others were nonmotile. The beating pattern, frequency, amplitude, 

and orientation were also variable and different from those of typical 9+2 cilia of 

ependyma. Transmission electron microscopy revealed the coexistence of 9+0 and 

9+2 configuration in neonatal CPEC. Overall, our results demonstrated striking 

molecular, functional, and ultrastructural heterogeneities in neonatal CPEC cilia, 

providing substantial novel insight on ciliary diversification. 

OFPMII 

PACAP 

1,2 1 1 1 1 

1 1 2 3 3 3 

1 

1 

2 3 

 

 

PACAPKO 

PACAP 

PACAP 

PACAP 

PACAP 

PACAP cAMP 

PKA 30 

PACAP AC 

 

AQP5 

PACAPKO AQP5 

PACAP 30 AQP5 

AQP5 

PACAP AC 

PACAP AC/cAMP/PKA 

AQP5 

 

OGPMI 

microCTmicroMRI 

 

1,2 3 4 5 6 7 

1 

JST 2 3 

4 

5 6 

7 

 

 

microCT microMRI 

 

 

microCTmicroMRI 

 

 

microCT CTmicroMRI 

 

 

CTMRI 

 

CT 

0.1 

mm 

 

 

OGPMI 

 

OGPMI 

HyperDry 

1 1 1 1 2 

1 

2 

3 

 

HyperDry HD 

HD FD 

 

φ25 mm 

Swinnex, SX0002500, 

50 ml 

 

1 ml 

50ml 1 ml 

185 mmAq730 mmAq 

133 mmAq 

 

730 mmAq FD=3.7 ul/min, HD=1.5 ul/min FDHD 

FD 

HD FD 

 

 

OGPMI 

 

1 2 2 2 2 

2 2 2 2 2 2 

1 1 1 1 

1 

2 

 

 

 

 

8 BALB/c 

4 mm 

 

3 0.1 mL 

Scion image 

6 

1.71 14 0.38 

3 

0.49 0.65 0.81 

7 0.80 6 0.86 

9 1.19 14 

3 14

102 

117 

OGPMII 

 

 

 

 

 

 

 

 

 

 

MMC 3T3Feader Fibrin 

SHEM DMEMF12 10% FBS 

 

 

 

 

 

 

 

 

Collagen Type 

 

 

OGPMII 

Muse iPS 

 

 

 

Muse Multilineagedifferentiating Stress Enduring 

SSEA3 

CD105 

 

Muse 

 

iPS 

iPS 

iPS 

Muse 

 

iPS 

Muse iPS 

Muse iPS 

 

 

Muse iPS 

 

OGPMII 

Monitoring DNA damage levels using γHAX detection in vivo 

1,2 Christophe Redon 2 William Bonner 2 1 

1 

2 National Institutes of 

Health 

DNA double strand break DSB is one major initial cause of genomic instability 

leading to cancer. The creation of a DNA DSB in eukaryotic cells is generally 

accompanied by the formation of hundreds of phosphorylated H2AX γH2AX 

molecules in the chromatin flanking the DSB site. Antibodies to γH2AX allow 

the visualization of a “focus” at the DSB site. Since it has been demonstrated that 

the numbers of γH2AX foci correlate directly to the numbers of DNA DSB, the 

detection of γH2AX is an attractive candidate for monitoring DNA DSB levels. 

For medical purpose, the ability to quantify DNA damage levels in cancer patients 

during ongoing drugs/radiation therapy is a useful tool for optimizing treatment. 

Recently, we evaluated γH2AX biodosimetry in a study using nonhuman 

primates subjected to totalbody irradiation. We successfully applied γH2AX 

assay to monitor DNA damage in vivo and assessed γH2AX as a biodosimeter of 

radiation exposure. In addition, we performed preliminary experiments to detect 

γH2AX in cancer patients during chemotherapy. Being able to routinely monitor 

DSB levels in individuals could provide useful tools for improving human health. 

OHPM 

 

 

 

 

 

116 

 

 

 

PHZ 

NBP 

 

TER119 

CD71 PCNA 

 

TER119 

 

RTPCR GATA1 

GATA2 

 

 

 

definitive 

 

OHPM 

MS 

 

 

 

 

 

 

 

Pentaerythritol triacrylate Ethyleneglycol dimethacrylate 

Methacrylic acidglycidyl methacrylate 

G02 

MS5 

CD34 CD34+ 

G02 MS5 MS5 

CD34+ 

12 

 

 

MS5 

CD34+ 

 

 

OHPM 

 

1 1 1 2 3 

Richard Wong 1 

1 

2 3 

 

RNA 

30 

 

AML 

NUP98 

HOXA9 

RAE1 

 

NUP98 

RAE1 

NUP98 RAE1 

NUP98 NUP98 

HOXA9 NUP98HOXA9 

NUP98HOXA9 RAE1 

AML 

RAE1NUP98 RAE1 

NUP98

117 103 

OHPM 

Two immunogenic passenger DC subsets in the rat liver with a 

distinct trafficking pattern and radiosensitivity 

 

 

To examine the phenotype, radiation sensitivity, trafficking pattern, and 

allosensitizing capabilities of conventional dendritic cell cDC subsets in 

liver graft rejection in rats, liver cDCs were examined by FACS or multicolor 

immunohistochemistry. 

We demonstrate two distinct immunogenic DC subsets. One is a CD172a+CD11b 

subset we previously reported that readily undergoes bloodborne migration 

to the recipient’s secondary lymphoid organs inducing systemic CD8+ Tcell 

responses. Another is a CD172a+CD11b+ subset that steadily appear in hepatic 

lymph. After transplantation, this subset further migrates to the parathymic lymph 

nodes, regional peritoneal cavity nodes, or partly persist in the graft and induces 

CD8+ Tcell responses there. Irradiation completely eliminated the migration and 

immunogenicity of the first subset, but only partly suppressed the migration of the 

second one. In this situation the grafts were acutely rejected and intragraft CD8+ 

Tcell and regulatory Tcell responses were unchanged. 

In conclusion rat liver cDCs contain at least two distinct immunogenic passenger 

subsets; a radiosensitive bloodborne migrant and relatively radioresistant lymph 

borne migrant. 

OHPM 

 

1,3 1,2 3 1 

1 

2 3 

 

 

CT SMA 

30 

2 

2 

 

 

CT -SMA 

Image J Photoshop Elements9 

CTSMA 5° 

20 

20.12.9 mm CT Th12 12 L1 8 

CT 1.610.41/ 0.350.17SMA 

0.840.38/ 0.480.14CTSMA 0.780.17/ 0.130.10 

CT 0.370.12/ 0.400.15SMA 0.380.12/ 0.37 

0.12 CTSMA 10.8°8.6° 

CT SMA 3 15% CT SMA 

1 5% 20% 

 

OHPM 

 

1 2 

1 

2 

10 nm 

Z 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

OHPM 

 

 

 

 

pericyte 

Hashitani et al. 2011 

 

 

 

α 

 

cKit 

P 

ChAT 

 

 

 

 

 

 

 

 

OIPM 

α 

1 2 2 Gerald W Hart 3 2 

1 

1 

2 3 Dept. Biol. 

Chem., Johns Hopkins Univ. Sch. Med 

O 

N OGlcNAc 

 

 

 

GK OGlcNAc 

OGlcNAc 

α 4 

ATP synthase OGlcNAc 

115 

α 4 GK 

 

α 4 

 

OGlcNAc 4 in situ Proximity 

Ligation Assay 

OIPM 

L LFABP 

1 2 2 1 

1 2 1 

1 

2 

 

L 

LFABP 

LFABP 

LFABP 

LFABP Tg II 

AngII Ang II 

4 

 

 

LFABP 

LFABP LFABP 

LFABP 

LFABP

104 

117 

OIPM 

EDA 

 

 

 

 

 

TGFβ1 

 

TGFβ1 

TGFβ1 24 

FN EDA 48 α 

αSMA 

 

EDA 48 

αSMA 

TGFβ1 

FN EDA TGFβ1 

 

EDA 

 

OIPM 

Fertilization analyzed by live imaging in the mouse 

Kiyotaka Toshimori, Chizuru Ito, Kenji Yamatoya, Mamiko Maekawa, 

Yoshiro Toyama 

Dept. Anatomy and Developmental Biology, Grad. Sch. Med., Univ. Chiba 

In order to analyze the fertilization process, it is important to visualize the events 

continuously induced during spermegg interaction. We analyzed the fertilization 

process by live imaging in the mouse, focusing on the relationship between 

pronucleus formation and fate of inner acrosomal membrane protein Equatorin 

EQT. 

Eggs interacting with fertilizing sperm in vivo were removed from oviduct 

and analyzed by live images, including immunofluorescence microscopy with 

antibodies against various sperm components and EQTEGFPtransgenic mice, 

under Olympus IX71 microscope equipped with high resolution Roper and 

CSU razor system Yokogawa. Sperm head was internalized and completely 

decondensed, releasing EQT in the ooplasm; it was before the initiation of 

the tail entry. Female pronucleus formation, in which a second polar body is 

released into the perivitelline space, started a bit faster than the male pronucleus 

formation, which was induced in the fertilization cone. It took about 90min to 

start the fertilization cone formation after spermegg fusion. Other live images 

and corresponding structural changes at early spermegg interaction stage will be 

presented. 

OIPM 

 

 

1 3 1,2 1 1 

1 1,2 

1 

2 3 Dept. Pharmacology, 

Physiology and Toxicology, Marshall Univ. Joan C. Edwards School of Med. USA 

DecaBDE 

DecaBDE 

 

 

 

DecaBDE 

DecaBDE 

 

CTTN ERK 1/2SRC 

DecaBDE 

DecaBDE 

ICR 1-5 

12 

 

 

DecaBDE 

 

OIPM 

microRNA 

 

1 1 2 3 4 3 

2 3 

1 

2 3 

4 

 

microRNAmiRNA 

PE miRNA 

 

PE 

RNA GAIIx TaqManq 

Array in silico 

laser microdissection [LMD]realtime PCRWestern 

blot BeWo miRNA 

3’UTR 

1,000 

667 PE 22 

PE miRNA In silico 5 PE miRNA 

 

HSD17B1 PE HSD17B1 

mRNA 

PE miRNA miR210 miR518c 

LMD miRNA 

 

miR210 miR518c HSD17B1 

PE miRNA 

 

OEAMIII 

 

 

 

C3 

 

 

Lenhossék 

86, p9, 2011 

 

 

C1, 2 

Isl1 

Nkx2.2 

C3 Lenhossék 

 

2 

Phox2b 

 

 

 

 

OEAMIII 

NPY 

 

 

Y 

NPY NPY 

 

NPY 

 

 

NPY 

NPY 

80% NOS NPY 

40% 90% 

NOS NPY 

NOS TH 

NPY NOS ChAT 

NPY TH 

NPY 

ChAT/NPY NOS/NPY 

NOS/NPY

117 105 

OEAMIII 

 

 

 

 

 

 

 

 

PHAL 

 

 

SD T910 

2.5%PHAL 30 μm 

 

 

700 μm2.3 mm 

 

 

 

 

 

 

 

 

OEAMIII 

gastrin 

releasing peptide 

1 1 Andrew Dobberfuhl 2 Lesley Marson 2 

1 

2 Division of Urologic 

Surgery, School of Medicine, The University of North Carolina at Chapel Hill, 

NC, USA 

gastrinreleasing peptide 

GRP 

 

PRV 

SNB 

 

 

GRP 

PRV 

GRP 

PRV 2 

PRV/GRP 

GRP PRV SNB 

GRP 

SNB 

GRP 

 

OEAMIV 

TRPV 

 

 

 

 

CPECs 

 

TRPV4 

 

CPECs TRPV4 mRNA 

CPECs 

TRPV4 

TRPV4 CPECs 

 

TRPV4 GSK101670A 

CPECs 

CPECs 

CPECs 

TRPV4 

 

OEAMIV 

Hh 

 

 

 

Hh 

Hh Patched Ptc 

Smoothened Smo 

 

Hh 

 

Schwann SC Hh 

/SC 

 

SC SC Ptc Smo 

 

SC Hh 

Hh 

 

 

SC Hh 

 

OEAMIV 

AP Notch 

1 2 3 4 1 

1 

2 3 

4 

 

AP1 

 

 

 

 

 

AP1 

 

AP1 Notch 

Notch 

AP1 RNAi 

AP1 

CG8538 CG8538 AP1 

Aftiphilin dAP1 

CG8538p/dAftiphilin dAP1 

Notch 

 

OEAMV 

MAPA supports NMDAreceptor transport for synaptic plasticity 

1 1 2 2 

1 

1 

2 BSI 

Microtubuleassociated protein 1A MAP1A is one of the major components of 

the neuronal cytoskeleton. To examine the role of MAP1A, we generated mutant 

mice lacking MAP1A. Through analysis of their phenotypes, we found that 

MAP1A knockout mice exhibited severe memory disturbances. The MAP1A 

knockout neurons revealed reduced surface expression of NMDAreceptors 

concomitant with a decrease in NMDAdependent postsynaptic current and long 

term potentiation LTP. Reduced NMDA receptor transport underlay the altered 

receptor function. These results suggest that MAP1A supports the transport of 

NMDAreceptors and synaptic plasticity in neuronal dendrites.

106 

117 

OEAMV 

Homera regulates the activityinduced remodeling of synaptic 

structures in cultured hippocampal neurons 

1 2 1 1 1 

1 1 1 1 

1 

2 

 

Homer1a LTP seizure 

PSD95 Homer1cFactin 

 

1h 

 

4h8hshRNA Homer1a 

knock down 

Homer1a 

OEAMV 

PIPKα regulates neuronal microtubule depolymerase KIFA and 

suppresses elongation of axon branches 

1,2,3 1,2 1 4 4 

1 

1 

2 3 

4 

Neuronal morphology is regulated by the cytoskeleton. Kinesin superfamily 

protein 2A KIF2A depolymerizes microtubules MTs at growth cones and 

regulates axon pathfinding. The factors regulating KIF2A in neurite development 

remain elusive. Here, using immunoprecipitation with an antibody specific to 

KIF2A, we identified phosphatidylinositol 4phosphate 5kinase PIPK as a 

candidate membrane protein that regulates the activity of KIF2A. Yeast two 

hybrid and biochemical assays demonstrated direct binding between KIF2A and 

PIPKα. Partial colocalization of the clusters of punctate signals for these two 

molecules was detected by confocal microscopy and photoactivated localization 

microscopy. Additionally, the MTdepolymerizing activity of KIF2A was 

enhanced in the presence of PIPKα in vitro and in vivo, suggesting a novel PIPK 

mediated mechanism controlling MT dynamics in neurite remodeling. 

OFAMIII 

SNAP 

 

 

 

 

 

 

SNARE SNARE 

SNAP23 SNAP23 tSNARE 

 

 

 

SNAP23 SNAP23 

 

SNAP23 

 

CreloxP 

NestinCre 

2 

 

SNAP23 

 

OFAMIII 

 

 

 

 

 

 

 

 

GM130 

GBF1 

 

810 

 

 

 

GM130 GBF1 

GM130 GBF1 

 

 

 

 

OFAMIII 

 

1 1 2 

1 

2 

 

 

 

7 30 

15 

20 

 

15 

δ2 

15 

δ2 20 

δ2 

 

α2 15 

20 

 

 

OFAMIII 

Input pathway and target cell typedependent regulation of synaptic 

AMPAR subunits in hippocampal CA region 

1 2 3 3 3 

1 

1 

Department of Anatomy, Hokkaido University Graduate School of Medicine, 

2 

Department of Anatomy, Kitasato University School of Medicine, 3 Department of 

Cellular Neurobiology, Brain Research Institute, Niigata University 

The AMPAtype glutamate receptor AMPAR is a tetramer of GluA subunits 

GluA1A4. Subunit combinations and contents of synaptic AMPAR are the 

major determinants of physiological properties of glutamatergic synapses. In 

the present study, the composition of synaptic AMPAR was investigated using 

subunitspecific antibodies and riboprobes in the mouse hippocampal CA1. 

Multiplelabeling in situ hybridization revealed that the subunit combination 

in pyramidal cells was GluA1, GluA2, and GluA3. Interneurons were high for 

GluA1, GluA3, and GluA4, and almost negative for GluA2. Exceptionally, 

parvalbumin PVpositive cells expressed all four subunits at high levels. 

Quantitative immunogold analyses revealed that labeling densities in Schaffer 

collateralCA1 pyramidal cell synapses were much higher than those in perforant 

path synapses, showing input pathwaydependent distribution. Synapses on PV 

positive cells displayed 23 times higher labeling densities for all four subunits 

than those on PVnegative interneurons. These results indicate that the subunit 

combinations and content of synaptic AMPAR are differently regulated by input 

pathway and target cell typedependent manners.

117 107 

OFAMIV 

 

 

1 1 1 2 3 

4 3 1 1 

1 

2 3 

4 

1 Muse 

 

Muse 

 

Muse 

neoblast 5 

 

Muse 

 

Muse 

 

 

3 

2 

Muse 

 

 

Muse 

 

 

OFAMIV 

 

 

 

 

 

 

 

 

 

 

Pax7 

 

Pax7 

in vitro 

 

 

 

 

 

 

 

OFAMIV 

 

1,2 3 1,2 1,2 3 

1,2 

1 

II 2 

3 

 

Shi 

 

2004, 2009 

 

 

 

CD146 fluorescent activate cell sorter 

in vitro 

 

 

 

CD146 

 

CD146 

CD146 

CD146 

2 

CD146 

 

OFAMV 

BMP dexamethasone 

1,2 1,2 1,2 

1 

1 2 

 

dexamethasone Dex 

Dex 

 

Dex 

 

ROBC26 C26 BMP2 

 

C26 BMP2 Dex 

 

Dex C26 

 

BMP2 

OsterixOSX Dex 

BMP2 OSX OSX 

Dex 

Dex C26 

BMP2 Dex 

 

OFAMV 

 

 

1 1 2 3 4 5 

6 1 4 7 

1 

2 

3 4 

5 JAXA ISS 6 

7 

 

 

 

 

 

2010 5 ISS 

 

 

STS132ISS 

86 

 

Flight μG, FμGFlight 1G, F1G 

Ground 1G 3 FμG F1G 

 

FμG 

 

 

 

OFAMV 

 

 

 

basolateral ventral 

ventral RB 

AR 2 

ventral 

AP 

Unroofing ventral 

AP 

TEM 

AR RB 

AR ventral 

AR 

 

RB 

ventral 

AP 

RB AR 

RB AP 

 

AR integrin endocytosis

108 

117 

OGAMI 

 

 

1,2 1 Takako Kondo 2 Eri Hashino 2 

1 

2 Dept. OtolaryngologyHNS, Indiana 

Univ. Sch. Med. 

95 

 

 

Wnt 

 

 

in vivo Tlx3 

 

 

Tlx3 

 

Tlx3 

Tlx3 Wnt 

 

 

Tlx3 

Tlx3 

 

OGAMI 

 

1 1 1 2 1 2 

1 1 2 1 

1 

2 

 

ES iPS 

 

 

 

 

 

Multilineage differentiating stress enduring Muse cell 

 

Muse ES 

Cluster Cluster 

3 

 

3 

 

SSEA3 

 

Muse 

 

 

OGAMI 

 

1 2 1 1 3 1 

1 

2 3 

 

ES inner cell mass 

 

 

 

 

 

 

3 

 

 

Oct3/4, OctA, Klf4, Nanog, cMyc, BCRP, 

Sox2, CK5, vimentin 

vimentin 

 

vimentin Oct3/4 

23 ALP 

 

 

 

OGAMI 

 

1 2 1 

1 

2 

 

 

 

 

 

 

 

 

 

6 

9 

10 μl 

 

10 

 

 

2 mm 

5 mm 

2010 mm 

 

OGAMII 

The application of antimicrobials or glycogen accelerates the pulpal 

regeneration of replanted molars in mice 

Angela QuispeSalcedo, Hiroko IdaYonemochi, Hayato Ohshima 

Div. of Anatomy and Cell Biology of the Hard Tissue, Dept. of Tissue 

Regeneration and Reconstruction, Niigata Univ. Grad. Sch. of Med. and Dent. Sci. 

The intentionally prolonged time for the completion of tooth replantation 

induces bone formation, expanded inflammatory reaction, or fibrous tissue 

formation in pulp tissue. This study aims to clarify the effect of a combination 

of metronidazole, ciprofloxacine, and minocycline 3Mix or enzymatically 

synthesized glycogen ESG solution on the pulpal healing of replanted teeth. 

After extraction of the upper first molars of mice, the teeth were immersed in 

3Mix solution at four different concentrations standard, 50%, 75%, and 90% 

during 5 to 60 minutes with or without the use of a transfer solution PBS; and for 

60 min in ESG solution in addition to PBS alone control. Immunohistochemistry 

for nestin verified tertiary dentin formation 1 week after operation in the 3Mix 

groups and at 2 weeks in the ESG group. In contrast, no tertiary dentin formation 

was observed at 2 weeks in the control. Standard concentration of 3Mix induced 

severe ankylosis of the replanted tooth, in spite of the accelerated dentinogenesis. 

In conclusion, the application of 3Mix or ESG promotes the pulpal regeneration of 

replanted teeth, although the 3Mix may induce severe damage to the periodontal 

tissue. 

OGAMII 

 

1 2 1 

1 

 

2 

 

 

 

ESG 

ESG 

ESG 

 

 

ICR ESG 2 mg/mLESG 

3 

3714 

 

ESG 

 

ESG 

14 ESG 3 

 

ESG DSPP 

ESG

117 109 

OGAMII 

BrdU 

 

 

 

Labelretaining cells 

LRCs 

ICR 3 BrdU 5 

 

1 2 EDTA Nestin 

OPN BrdU TUNEL in situ 

Dsp 

1 TUNEL 

 

LRCs 35 

Nestin LRCs 

Nestin 7 

OPN 

DspmRNA 

LRCs 

 

OPN 

 

OGAMIII 

Cx NFATc 

1 2 1 3 

1 

2 3 

Cx45 

Cx45 

Cx45 

 

 

Cx45 

 

Cx45 

Cx45 

Cx45 

 

NFATc 

NFATc VEGF 

Cx45 

NFATc 

 

 

OGAMIII 

FGF 

1 2 

1 

2 

GDNF 

 

FGF 

1213 

FGFcanonical, 15 FGF 7 

FGF 3 

GDNF Ret, GFRα1 

FGF5, FGF9, 

FGF17, FGF18, FGF22 FGF9 

10 FGF9 

Ret, GFRα1 GDNF 

 

FGF9 FGF9 

FGF 

FGF9 

FGF9 

 

 

OGAMIII 

An attempt to identify novel ciliary genes by database comparison 

 

 

Cilia and flagella are hairlike organelles that project from the cell surface and 

have been implicated in a diversity of cell functions. Although a number of 

putative ciliary genes have been identified by several global analyses, little is 

known about the functions of these genes. To gain a better understanding of ciliary 

biology, we selected ciliary candidate genes from comprehensive ciliary protein 

data sets, named ciliome, and analyzed their antisenseknockdown phenotypes 

in medaka embryos. To maximize quality hits, we selected genes expressing 

in ciliary organs based on the zebrafish expression database. Comparing the 

expression among 5 ciliary organs, we found that 18 genes are commonly 

expressed in more than 4 organs out of the above 5. Comparing these genes with 

ciliome database, 5 out of the 18 genes were recorded in ciliome database. We 

therefore preliminary examined the medaka antisenseknockdown phenotypes of 

2 of the 5 genes. One revealed hydrocephalous and the other showed cystic kidney 

phenotype: both of which are ciliadefective phenotypes. This data suggests that 

our strategy is a promising approach to understanding various roles for ciliome 

proteins. 

OHAMI 

 

1 1 2 1 

1 

2 

 

 

 

 

 

 

 

 

58 

Rmax 

10 mm 

 

 

 

 

27 

 

 

 

 

OHAMI 

On the phylogeny of the zygomatic arch, and its relationships among 

foramina and the bony wall of the orbit 

1,3 1 2 2 3 

3 4 4 4 4 

1 

Kanagawa Dental College, 2 Edogawa Hospital, 3 Dept. Radiology, Yokohama City 

Univ. Sch. Med., 4 Dept. Neurosurgery, Yokohama City Univ. Sch. Med. 

The existence of the arcus zygomaticus is a characteristic of mammalian skulls. 

The arches provide surface for the masseter muscle to attach, and the canalis 

zygomaticus stores the passage of the zygomatic nerve. Romer & Parsons 

surmised that the arches are a last remnant of the former lateral walls of the 

reptilian skull. Only Simiiformes Catarrhini and Platyrrhini has the perfect bony 

orbital wall, whereas the Prosimii, Perissodactyla, and Artiodactyla lost the rear 

wall and floorbord of the bony orbit, and the Carnivora lost the lateral posterior 

bony wall of the orbit. However Hystricomorpha has another pair of arches on 

the lateral walls of the greatly enlarged infraorbital foramen. This structure seems 

to us a new frontal and rear expansion and reinforcement of the zygomatic arch. 

In the Simiiformes, the Fossa temporalis has a relatively large volume so that the 

brain case has become a high structure. Therefore it was not necessary for the 

zygomatic arch to enlarge in front and behind.

110 

117 

OHAMI 

 

 

 

 

 

 

 

5 10 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

OHAMI 

 

1 2 2 3 

1 

2 

3 

3 

 

 

 

 

 

 

 

CT 

CG 

CT 

CG3dsMAX CT 

CG 

 

CG 

CG 

 

 

CG 

 

 

OHAMII 

Persistent median artery: Relationships to palmar arches and median 

nerve branches 

Nabil Eid, Yuko Ito, Yoshinori Otsuki 

Osaka medical college 

Two cases of unilateral persistent median artery PMA were detected during the 

dissection of 25 cadavers. The first case was a 75yearold man. A dissection of 

his left upper limb revealed a PMA piercing both the median nerve MN and 

the anterior interosseous nerve. The PMA coursed distally, deep to the transverse 

carpal ligament TCL, forming a medianulnar pattern of complete superficial 

palmar arch SPA. The PMA was superficial to two nerves at the distal edge of 

the TCL; the extraligamentous recurrent thenar RT branch of MN and the third 

common digital nerve TCDN. The other case was an 80 yearoldfemale. Her 

left forearm showed a high origin of the radial artery from the brachial artery with 

trifurcation of the latter into PMA, common interosseous and ulnar arteries. The 

PMA passed deep to the TCL forming radialmedian ulnar pattern of SPA. Both 

the transligamentous RT branch of MN and the TCDN passed deep to the PMA 

inside the carpal tunnel, before the crossing of the latter nerve ventral to the SPA 

on its way to the digits. The relationships of the PMA to MN branches may have 

important implications regarding the diagnosis and treatment of MN compressive 

syndromes. 

OHAMII 

 

 

 

 

 

 

 

 

 

10 20 

 

 

 

 

 

 

 

 

 

 

 

 

 

OHAMII 

Thiel 

1 2 2 2 3 

2 

1 

2 2 3 

 

 

 

Thiel 5 9 

 

12 

 

3 4 7 2 : 

4 

 

5 

 

67 

 

TFCC 

Thiel Graz Thiel 

 

variation 

 

 

OHAMIII 

 

 

 

 

 

 

 

11 20 

 

 

3 

 

 

 

 

 

 

 

 

variation

117 111 

OHAMIII 

 

1 2 2 2 2 

2 2 2 2 1 

1 

2 

2011 

39 

 

VSD 

15 12 mm 

 

7 cm 

 

 

 

 

 

64 mm 

1 

 

 

5 mm 4 E 

7 

6 

 

OHAMIII 

 

 

 

deep circumflex iliac vein DCIV 

DCIV 

external iliac 

artery EIA 

 

EIA DCIV 

 

20092011 38 72 

DCIV EIA 65 

242 8 DCIV 

3DCIV EIA 

 

63% 

 

Edwards and Robuck 1947 lateral 

femoroiliac circle LFIC 

DCIV 2 

LFIC 

OIAMI 

CT 

 

1 2 1 

1 

2 

 

 

 

 

5 

CT 

48 5798 

24 4 

2 CT 

 

5 

 

> 

14 2 

560 90 4 

20 2 

9 

 

 

 

OIAMI 

 

 

 

 

P6 SAMP6 

 

SAMP6 

 

SAMP6 

18 SAMP6 8 18 

R1 SAMR1 8 

CT RmCT, RIGAKU, Japan 

18 

TRI/3DBON RATOC, Japan 

 

BV/TV Tb.N SAMP6 SAMR1 

Tb.Th 

BV/TVTb.NTb.Th 

SAMP6 

 

 

 

OIAMI 

klotho/ 

1,2 1 1 1 3,4 

1 2 1 

1 

2 

3 

4 

klotho klotho/ Ca/P 

 

klotho/ 

 

5 klotho/ 

von Kossa ALP, 

DMP1, MGP, osteocalcin DMP1 

 

 

klotho/ ALP 

klotho/ 

DMP1 osteocalcin MGP 

DMP1 

klotho/ 

klotho/ 

DMP1 osteocalcin 

 

OIAMI 

 

1,2 1 1 1 2 

1 

1 

2 

1960 Bélanger PTH 

osteocytic osteolysis 

 

FGF23 

PTH 

 

12 ICR human PTH 134; 80 mg/ 

kg 6 

von Kossa , a3,d2 

FGF23 

PTH 

von Kossa 

FGF23 d2 

a3 

 

PTH

112 

117 

OIAMII 

Osteogenic culture 

 

 

 

 

 

 

 

in vitro 

 

hMSC12 

2 

 

 

 

 

 

in vitro 

 

 

in vitro 

 

OIAMII 

PKR 

1 1 1 2 

1 

2 

PKR 

 

PKR PKR 

PKR 

2 2AP RAW264.7 

RAW RANKL TRAP 

 

mRNA RTPCR Real time PCR 

PKR RAW K296S 

2AP RAW RANKL TRAP 

RANKL pc 

TRAP PKR 

TRAP 

MFRDCSTAMP, Cathepsin K, CTR 

2AP PKR NFκB NFATc1 

2AP PKR STAT1 

PKR 

NFκB STAT1 

OIAMII 

WT RNA 

1 2 1 

1 

2 

WT1 Wilms’ 

Zinc finger 

 

 

WT1 

RNA 

in situ hybridization 

WT1 RNA 

WT1mRNA 

WT1 RNA 

RAWD 

WT1 

WT1 WT1 

RNA RAWD WT1 

WT1 

RNA 

RNA WT1 

 

 

OIAMIII 

septoclast 

EFABP 

1 1 1 1 2 

1 

1 

2 

FABPs 

FABPEFABP 

septoclast 

 

4% paraformaldehyde EDTA 

 

 

EFABP septoclast 

 

EFABP 

EFABP septoclast 

EFABP 

 

 

septoclast 

EFABP 

EFABP 

 

OIAMIII 

 

 

1 2 2 2 

1 

2 

 

10 

 

 

HCl 

 

JXA8900 EPMA 

 

 

CaP 

 

ONa CaP 

CaP 

 

 

 

 

 

OIAMIII 

 

1,2 1 1 1 4,5 

1 2 3 1 

1 

2 

3 

4 5 

 

periostin/ 

periostin/ 

 

periostin/ 

 

MMP1,2, F4/80 

 

 

shear zone MMP1 F4/80 

 

periostin/ MMP1MMP2F4/80 

shear zone 

 

 

periostin/ 

 

shear zone

117 113 

ODPMI 

Large Maf 

 

 

Large Maf 

MafAMafBcMafNRL 

4 Maf 

MafAMafB 

cMaf MafA 

β 

 

MafA 

MafA 

MafB 

MafB 

 

α β 

Large Maf 

ODPMI 

 

 

Mohamad Reza 

 

5 

FS FS 

 

 

 

 

FS 

FS GFP S100bGFP TG 

FS DNA 

 

GFP FS 

S100b protein, bFGF, Vime 

 

MKMidkine 

PCR MK GFP 

in situ hybridization FS MK mRNA 

MK FS 

 

ODPMI 

Notch 

1 1 1 2 

1 

2 

 

Juxtacrine Notch 

 

Hoechst 33342 

Notch 

Notch 

 

Notch 

In situ hybridization 

4 Notch 

2 marginal layer 

Notch 

 

Notch 

Notch marginal layer 

 

 

ODPMI 

 

Dini RamadhaniDepicha Jindatip 

 

FS 

 

FS ECM 

gap junction 

 

FS 

FS FS FS FS 

3 realtime PCRin 

situ hybridization ISHFS 5 

FS 

ECM I III 

FS 

FS 

ISH 

RGS5 FS 

FS 

 

ODPMII 

Expression of laminin isoforms during anterior pituitary development 

in the rat 

Dini Ramadhani 

 

Laminin isoforms LMs are major basement membrane components in anterior 

pituitary. Currently 19 LMs have been identified in vivo, and they have multiple 

functions, not only as a scaffold but also as induction of cell differentiation 

and cell signalling. However, LM function in anterior pituitary development 

has not been studied. The purpose of this study is to determine the laminin 

isoform expression during the anterior pituitary development. RTPCR, in situ 

hybridization ISH, and immunohistochemistry IHC were used. RTPCR and 

ISH analysis showed the laminin alpha 1, 3, and 4 gene expressions in adult 

anterior pituitary, while only laminin alpha 5 mRNA was expressed in early stage. 

Correlating with ISH data, IHC showed that laminin protein was first expressed 

around the pituitary in early stage and it was gradually expressed within the 

pituitary as vasculatures are formed in the gland. The results indicate that the 

expression of laminin isoforms varies during anterior pituitary development in 

the rat. This study may provide basic knowledge for further study on analysis of 

laminin functions in the anterior pituitary gland. 

ODPMII

114 

117 

ODPMII 

 

OEPMVI 

 

 

1,2 1,2 1,2 1,2 1,2 

1 

2 

 

 

 

 

 

 

 

 

 

IdaEto et al., Neurosci. Lett., in press 9 

Wistar 15 

 

 

2 

 

 

OEPMVI 

Developmental Origins of Health and Disease DOHaD 

 

Randeep Rakwal 

 

 

DOHaDDevelopmental Origins of Health and Disease 

 

 

ADHD 

 

50% 

1018 17 

18 mRNA 

DyeSwap DNA 

250 1300 

140 

GABA A neuromedin BStat1 

 

 

T 

 

 

OEPMVI 

 

1 - 1 1 1,2 1 

1 

2 

 

PTSD 

 

 

maternal separation: MS 

 

 

cFos 

MS 2 3 / 

MS repeated MS: RMS 2 MS 

single MS:SMS cFos 

RMS SMS cFos 

RMS 

SMS MS 

 

 

MS 

OEPMVI 

q E/I 

1,2 1,4 1 1 1 

3 3 5 1,2,6 

1 

2 

3 

4 Heidelberg University, Germany 5 

6 

JST,CREST 

 

 

 

15q11q13 

20 

 

 

 

 

/ Excitation/Inhibition, E/I 

 

E/I 

 

E/I VGLUT1 Vesicular glutamate transporter 1 

VGAT Vesicular GABA Transporter 

VGAT VGLUT1 

 

E/I 

OEPMVII 

 

 

 

 

DNA 

BromodeoxyuridineBrdU 

2 

 

BrdU 2 

 

 

 

1 2 

 

Notch1 

Notch1 

 

Notch1

117 115 

OEPMVII 

 

1 1 2 2 1 

1 

2 

 

 

 

 

experimental 

autoimmune encephalomyelitis: EAE EAE 

 

C57BL/6 Myelin oligodendrocyte 

glycoprotein 3555 MOG3555 

EAE 

 

 

 

 

EAE 

 

 

 

EAE 

OEPMVII 

RAGE 

 

 

 

Receptor for advanced glycation end productsRAGE 

 

RAGE 

RAGE 

 

BCCAORAGE 

RAGE esRAGERAGE 

3 BCCAO 

 

BCCAO 12 

3-7 RAGE 

RAGE esRAGE 

24 7 

12 

 

RAGE 

 

 

OEPMVIII 

Fasting and highfat diet alter histone deacetylase expression in the 

medial hypothalamus 

 

 

Increasing attention is now being given to the epigenetic regulation of animal 

and human behaviors including the stress response and drug addiction. Histone 

deacetylases HDACs are involved in the epigenetic control of gene expression 

and alter behavior in response to a variety of environmental factors. In response 

to fasting and highfat diets, the expression of both orexigenic and anorexigenic 

neuropeptides change in the medial hypothalamus, a crucial region for feeding 

behavior and body weight homeostasis. However, the mechanism by which gene 

expression is modulated in the medial hypothalamus remains to be clarified. 

Here, we examined the expression of HDAC family members in the medial 

hypothalamus of mice in response to either fasting or a highfat diet. In response 

to fasting, HDAC3 and 4 expression levels increased while HDAC10 and 

11 levels decreased. Four weeks on a highfat diet resulted in the increased 

expression of HDAC5 and 8. Therefore, HDACs may be implicated in altered 

gene expression profiles in the medial hypothalamus under different metabolic 

states. 

OEPMVIII 

 

1 2 3 1 

1 

2 3 

 

 

 

 

1 2 

3 

1 

2 

PGP9.5 

CGRP 

1/3 

Aδ 

C 

1/21/3 

 

CGRP C 

 

 

 

OEPMVIII 

PAM 

1,2 1 1 

1 

2 

PAMPressure Application Measurement 

Randall Selitto 

 

 

PAM 

5 

6 

 

NSAIDs 10 1 2 

3 

NSAIDs PAM 

 

 

cFos 

 

OFPMVI 

 

 

1 Watson Eileen 2 1 

1 

2 Dept. Oral Health Sciences, University of 

Washington 

Proteaseactivated receptor PAR 

G 7 PAR 

 

PAR 

PAR RTPCR PAR2 

PAR2 SLIGRLNH 2 

[Ca 2+ ] i Ca 2+ [Ca 2+ ] i 

 

Ca 2+ noncapacitative calcium entry NCCE 

 

Ca 2+ NOS 

LNAME 

ADP 

calmodulin kinase II CAMK II 

calmodulin [Ca 2+ ] i 

PAR2 [Ca 2+ ] i 

NOS 

Calmodulin CAMK II

116 

117 

OFPMVI 

Biodiversity and hepatic stellate cells 

Haruki Senoo 1 , Yoshihiro Mezaki 1 , Mitsutaka Miura 1 , Katsuyuki Imai 1 , 

Kiwamu Yoshikawa 1 , Mayako Morii 1 , Mutsunori Fujiwara 2 , Rune Blomhofff 

3 

1 

Dept. Cell Biology and Morphology, Akita Uni. Grad. Sch. Med., 2 Divis. Clinical 

Pathol, Japanese Red Cross Med. Center, 3 Dept. Nutrition, Inst. Basic Med. Sci, 

Fac. Med., Univ. Oslo 

Hepatic stellate cells HSCs exist in the space between hepatocytes and 

sinusoidal endothelial cells, and store 5080% of vitamin A in the whole body 

as retinyl palmitate in lipid droplets in the cytoplasm. We have performed a 

systematic characterization of the hepatic vitamin A storage in animals of the 

Svalbard archipelago and Greenland, and compared that in polar bears kept in 

zoos. The top predators contained about 1020 times more vitamin A than all other 

arctic animals studied as well as their genetically related continental top predators. 

This massive amount of hepatic vitamin A was located in large lipid droplets in 

HSCs. The contents of retinyl esters in the liver for polar bear kept in zoos were 

dependent on vitamin A content in the diet. The development of such an efficient 

vitamin Astoring mechanism in HSCs may have contributed to the survival of 

top predators in the extreme environment of the arctic. HSC that has capacity 

of taking up and storing of a large amount of vitamin A plays pivotal roles in 

maintenance of food web, food chain, biodiversity, and eventually ecology of the 

arctic. 

OFPMVI 

A 

1 1 1 1,2 1 

1 1 

1 

2 

 

A A 

A perilipin 2/ADRPperilipin 3/TIP47 

ADRP TIP47 A 

A TIP47 

 

A A 

ADRPTIP47 

A 

ADRPTIP47 

A 24 A TIP47 

7 A 

TIP47 A A 

 

A TIP47 

A A 

24 7 TIP47 A 

A TIP47 

ADRP A 

OFPMVI 

IEL DNA 

 

 

CD3 IEL 

30 IEC DNA 2 

IEC 

IEC DNA DNA 

DNA IEL IEC 

DNA IEL 

BGrB IEL GrB 

IEC DNA GrB 

IEC DNA Pfn/GrB 

DNA 

IEL Pfn GrB 

IEL Pfn 

DNA 

GrB DNA 3 

IEL IEC DNA 

Pfn/GrB Pfn GrB 

DNA 

 

OFPMVII 

SU p 

 

 

 

Src SU6656 

5F9A 

 

DNA BrdU DNA 

 

BrdU 

S 

 

 

2 2 

SU6656 

 

p53 siRNA p53 

SU6656 p53 

Nutlin SU6656 

SU6656 p53 

p53 

 

OFPMVII 

 

1 1 1 1 2 

1 

2 

 

 

 

 

2 

 

 

 

1 

A single 

cKit 

A single A aligned cKit 

A1 A single A paired 

 

 

Np95 

 

 

OFPMVII 

HORMAD 

 

 

HORMAD1HORMAD2 

Hop1 HORMA 

 

HORMAD1 HORMAD1 

HORMAD2 

HORMAD2 

HORMAD2 

 

XYbody 

HORMAD2 

SPO11 HORMAD2 

SPO11 

HORMAD2 



 

 

 

HORMAD2

117 117 

OFPMVIII 

α 

 

 

 

 

 

 

 

 

NPC1L1 

 

LC3II 

NPC1L1 

 

Lamp1 Ragulator 

p18 Lamp1 mTOR 

 

 

 

α1 ATZ 

 

α1 

 

OFPMVIII 

E HRD 

 

 

 

 

ERassociated 

degradation; ERAD unfolded 

protein response; UPR E3 

HRD1 ERAD UPR 

 

ERAD UPR 

HRD1 

 

ICR 6 4% 

HRD1 Abgent1.25 μg/ml 

 

HRD1 

 

 

 

HRD1 

 

OFPMVIII 

 

1 2 2 

1 

2 

 

[Aim] We investigated the influence of the adrenal glands over the preservation 

of gap junctions between folliculostellate cells in the anterior pituitary glands of 

rats. [Maerials and Methods] 60 dayold WistarImamichi strain male rats were 

prepared. The animals were divided into six groups: untreated control, untreated 

but given 0.9 % NaCl drinking water, castrated, adrenalectomized, castrated and 

adrenalectomized, and castrated and adrenalectomized with the administration 

of testosterone. Five rats from each group were killed 1, 2, 3, 4, 5, 6 and 7 

days after the operation, and prepared for observation by transmission electron 

microscopy. [Results] The simultaneous removal of adrenal glands with castration 

resulted in the significantly decreasing effects of gap junctions, and the additional 

administration of testosterone to these rats could compensate for the decreasing 

effects of gap junctions, while few mitotic hormoneproducing granular cells 

were found. [Conclusion] These observations indicate that the preservation of gap 

junctions between folliculostellate cells is mainly dependent on the testosterone 

from both testes and adrenal glands in adult male rats. 

OGPMIV 

NEX 

 

 

 

 

 

 

 

 

 

 

 

NEX Math2 bHLHtype transcriptional factor 

Cre 

 

NEX 

 

OGPMIV 

FLRT Unc 

1,2 Falko Hampel 2 3 Daniel del Toro 2 

Manuela Schwark 4 Elena Kvachnina 4 Martin Bastmeyer 5 3 

Victor Tarabykin 4 Joaquim Egea 2 Ruediger Klein 2 

1 

2 MaxPlanck Institute of 

Neurobiology 3 4 MaxPlanck 

Institute for Experimental Medicine 5 Karlsruher Institute fuer Technologie 

 

 

 

FLRT 

Unc5 

FLRT2 Unc5D UL2 

 

Unc5D UL2 

RNA 

FLRT2 VVI UL2 

IV Unc5D 

FLRT2/ UL2 

FLRT2 UL2 

 

OGPMIV 

Implications for cooperative versus noncooperative actions of the 

subunits of NatB, Mdm and Nat in mouse embryonic brain 

Kyoji Ohyama 1 , Kunihiko Yasuda 1 , Kazuko Onga 1 , Akira Kakizuka 2 , 

Nozomu Mori 1 

1 

Dept. of Anatomy and Neurobiology, Grad. Sch. Biomedical Sciences, Nagasaki 

Univ., 1124 Sakamoto, Nagasaki 8528523, Japan, 2 Lab. of Functional Biology, 

Kyoto Univ. Grad. Sch.Biostudies, Kyoto 6068501, Japan 

The NatB complex, Nat5/Mdm20 acetyltransferase mediates Nacetylation to 

control cell cycle progression and actin dynamics in yeast. As yet, little is known 

about their expression patterns in multicellular organisms. Here we show that 

Mdm20 is highly expressed in mouse embryonic brain. At E11.5, Mdm20 was 

widely expressed in both neural progenitors and early differentiating neurons, 

whereas Nat5 was expressed in Sox1/3+/Mdm20+ neural progenitors. By E14.5, 

both Mdm20 and Nat5 were downregulated in ventricular zone neural progenitors, 

whereas both protein expression were found in differentiating neurons and 

maintained at E18.5 in derivatives of these cells, such as midbrain dopaminergic 

DA neurons. Intriguingly, our data also showed that Mdm20 is not always co 

expressed with Nat5 in all differentiated neurons, for example deep cerebellar 

neurons. Moreover, Mdm20 is also not necessarily colocalised with Nat5 within 

Nat5+/Mdm20+ midbrain DA neurons. Given that Nat5 is only a known member 

of Nat family protein that interacts with Mdm20, our data imply that Mdm20 may 

function either with an unidentified Nat protein partners or possibly in a Nat 

independent manner.

118 

117 

OGPMIV 

 

 

1 1 1,2 1,3 1,4 

1 

2 3 

4 

CS 

 

 

CS 

 

IgG 

IgM 

CS 

IgM 

IgM Hierck 1994 

CS IgM 

CS 

ABC 

CS 

CSA CSD CS56 

 

IgM 

 

OGPMV 

Hes 

 

1 2 3 3 

1 

2 3 

 

NotchHes 

Hes1 / 

Hes1/ 

SCG 

12.5 E12.5 E13.5 SCG 

E14.5 C1C3 

Hes1/ E13.5 SCG 

E17.5 SCG 

26.3% 

SCG E13.0 3 

SCG E13.5 SCG 

Hes/ 

E17.5 

52.5% H3 SCG 

Hes1/ 

Hes1 SCG 

 

OGPMV 

M. iliotibialis cranialis 

 

 

 

 

 

 

 

M.iliotibialis cranialis 

 

E5 

 

E6 E7 

E8 

E9 L1 

L2 

E5 

 

 

 

OGPMV 

 

 

1 2 2 2 

1 

2 

 

 

 

 

 

 

 

CL57BL/68 

50 mm 500 mm 0 45 90 

 

BrdU 1 mg/ml 2 

BrdU 

BrdU 

 

 

 

 

OGPMVI 

Differential effect of aberrant expression of ectodysplasinA receptor 

edar on scales and jaw and pharyngeal dentition of medaka 

Otto Baba 1 , ADSL Atukorala 1 , Keiji Inohara 2 , Makoto Tabata 1 , Kiyoshi Mitani 3 , 

Yoshiro Takano 1 

1 

Biostructural Science, Graduate School, Tokyo Medical & Dental University, 

2 

Biological Information, Tokyo Institute of Technology, 3 Biological Science, 

Graduate School of Frontier Science, University of Tokyo 

To find out the role of ectodermal cell signaling in scale and tooth formation in 

teleosts and thereby to gain insights in evolutionary origin of teeth, we analyzed 

scales and teeth in rs3 medaka mutant characterized by reduced scale numbers 

due to aberrant splicing of ectodysplasinA receptor edar. Results: In normal 

medaka, we confirmed edar signals in the enamel epithelium at early tooth 

development in both jaw and pharyngeal dentition. The signal was gone once 

mineralized matrix had deposited. In adult rs3, drastic loss of scales 83 % 

and teeth occurred in both oral 43.5 % and pharyngeal 73.5 % dentition. 

Remaining scales were irregular and much larger in size relative to those of wild 

type. In contrast, there was no abnormality in size and shape in the remaining 

teeth of rs3. ThreeD analyses of embryonic development of pharyngeal regions 

indicated that pharyngeal tooth formation preceded gill slit opening and showed 

no sign of ectodermal cell migration in pharyngeal endoderm and hence no direct 

evidence of ectodermal contribution to pharyngeal odontogenesis. Conclusion: 

These data support intrinsic odontogenic competence of rostral endoderm in 

medaka. 

OGPMVI 

DNA TGFβ 

 

1 1 2 3 

1 

2 3 

DNA 

RG108 MEE 

TGFβ3 KO 

 

RG108 ICR C57BL/6J MEE 

 

100 C57BL/6J 

TGFβ3KO RG108 

 

in vitro ICR C57BL/6J 

MEE RG108 

C57BL/6J MEE ICR 

C57BL6J TGFβ3KO 

RG108 

ICR TGFβ3KO 

15.8 

 

DNA

117 119 

OHPMIV 

 

 

 

 

 

 

 

 

 

 

 

 

1 MAL 

 

2 

 

MAL 

 

 

 

 

OHPMIV 

D 

 

 

 

 

Saito et al., 2006, Steinke H et al., 2009 

 

3D 

 

5 

 

 

MRI LaserErgo Scanner 

 

3D 

 

 

3D 3 

 

Saito et al, Surg Radiol Anat 28, 228234, 2006 

Steinke H et al. Ann Anat 191, 408416, 2009 

OHPMIV 

 

1 2 3 3 4 3 

1 

2 3 4 

 

2 

19% 

 

 

1 

 

Gray’s Anatomy 

Gottshalk 1989 

3 

Akita 1993 

 

3 

 

 

 

1 0.8 

 

 

OHPMIV 

 

1 2 2,3 

1 

2 3 

 

 

7 

12 

3 

 

 

 

 

67 

33 

1/31/6 50 

50 

 

 

 

 

 

 

 

OHPMIV 

Threedimensional modeling of the architecture of the plantar 

muscles of the great toe 

Takamitsu Arakawa 1,2 , Anne Agur 1 

1 

Division of Anatomy, Department of Surgery, Faculty of Medicine, University of 

Toronto, 2 Kobe University Graduate School of Health Sciences 

The plantar muscles of the great toe are involved in many foot pathologies, 

including hallux valgus. Forces acting along their tendons are important 

considerations in analyzing gait patterns and arch stability. Muscle models have 

not been constructed at the fiber bundle level, but rather using a series of single 

or multiple line segments to represent a muscle. The purpose of this study is to 

construct, at fiber bundle level, a volumetric 3D model of the plantar muscle of 

the great toe. In situ digitization with a Microscribe TM G2DX digitizer of one 

formalin embalmed cadaveric specimen was used to develop a 3D prototype 

model of abductor, adductor, and flexor hallucis muscles. Autodesk ® Maya ® , with 

additional plugins developed in this laboratory, were used to create a model the 

digitized data. Using this technique, a detailed model at the fiber bundle level was 

constructed and used to visualize and quantify the musculotendinous architecture 

of the plantar great toe muscles. This prototype may be further developed into 

a contractile model that enables functional analysis in normal and pathologic 

scenarios. 

OHPMI 

The characteristic of the filiform and fungiform papillae of human 

tongue 

ShineOd Dalkhsuren, Kikuji Yamashita, Dolgorsuren Aldartsogt, 

Kaori Sumida, Shinichiro Seki, Seiichiro Kitamura 

 

Objective: We aimed to clarify whether there are the structurally differences of the 

filiform and the fungiform papillae of human tongue depending on their position 

and function. 

Methods: The tissue specimens were separated to the significant parts: the tip, the 

body and the root of tongue and processed with the scanning electron microscopy 

SEM JCM5700, Jeol, Tokyo, Japan and the light microscopy. 

Results and discussions: The tip of tongue is the first sensing place for taste 

stimuli and the most active moving side. Consequently, the free longbased 

fungiform papillae distributed equable densely and a few filiform papillae 

containing the less and strong hairs placed along the margin of the fungiform 

papillae. The body of human tongue is the main mastication area. And it contained 

the numerous filiform papillae which are composed of a lot of hairs for making 

the soft sponge structure and holding the tongue plaque. In the root, the both of 

filiform and fungiform papillae were changed responding to the frictional stress as 

the swallowing. 

Conclusion: The structural variations of filiform and fungiform papillae might be 

depended on their positional role.

120 

117 

OHPMI 

 

 

1 2 3 3 4 

4 1,3 

1 

2 2 3 

4 

 

 

Wistar 2 

2 

HRP 

FcRn IgG EEA1 

 

 

HRP 

 

 

 

FcRn IgG 

FcRn IgG FcRn 

 

 

 

 

OHPMI 

 

1,2 

1 

2 

 

19 24 

10 30 1 3 30 90 ddY 3 

3 Hthymidine, 3 Huridine, 3 Hleucine 1 

 

400 kV 

 

10 

1 8 

1-2 15 2 

DNA 7-14 

 

1 24RNA 

2-6 

2 

24 

 

 

 

 

OHPMII 

CRF 

1 1,2 1 1 1 1 

1 1 

1 

2 

 

 

FD 

IBS 

 

 

CRF 

 

 

1 2 Water avoidance WAS 7 

 

CRF 

WAS 

WAS CRF 

alphahelical CRFWAS 

CRF 

 

WAS FD 

WAS CRF FD 

 

OHPMII 

 

1 2 3 4 5 

1 

2 3 

4 5 

 

 

 

αmangostin 7585γmangostin 515 

F344 200 

mg/kg 1 2 

2000 4000 ppm 

500 ppm 

3 

8 

S GSTP 

2000 

4000 ppm GSTP 

 

GSTP 

 

 

OIPMIV 

tendon gel 

1 1,2 2 3 3 

4 5 

1 

2 3 

4 

5 

 

 

1 mm 

2 

40 

1 

10 tendon gel, TG 

TG 

 

TG 2 

2 11 62 nm n=550 

3 

12 40 

6314 nm n=166 6820 nm n=161 

2 TG 

TG 

TG 17.02 MPa 

TG TG 

327 nm n=214 

OIPMIV 

 

 

 

desminvimentin 

 

 

 


 

 


1216 ICR 

HE 


desmin 12 

vimentin 12 

12 

 

desminvimentin

117 121 

OIPMIV 

 

1 2 1 1 3 3 

1 

2 

3 

 

 

 

 

 

 

 

 

2 

 

 

OIPM 

 

 

 

 

 

 

2007 

SA 

 

SA 

 

 

 

 

SA 2 SA 

2 

 

SA 2 SA 

SA 

 

 

 

OIPM 

CT 

1 2 2 3 4 

1 

2 3 

4 

21 X CT 

22 

1 mm CT 

3 

 

 

 

10 CT 

 

 

 

3 

 

 

CT 

 

 

CT 3D 

 

OIPM 

 

1 2 2 2 2 

2 3 1 1 1 1 

1 

2 

3 

2008 

2009 

2010 

 

[ 

86: 3337 2011]1. 2. 

3. 4. 

3. 3 

 

 

 

 

 

 

 

 

 

OIPM 

Organization of the lens elasticity associating with accommodation 

in monkey eyes 

Mari Hiraoka 1,2,3 , Haruki Senoo 3 , Masahiko Takada 4 

1 

Koganei Eye Clinic, 2 Lab. Brain Develop, Tokyo Metropolitan Inst. Med. Sci., 

3 

Dept. Cell Biol. Morphol, Akita Univ. Grad. Sch. Med., 4 System Neurosci. Sect, 

Primate Res. Inst, Kyoto Univ. 

To understand the mechanism of accommodation, our dynamic connective tissue 

theory revealed the zonule inside of capsule may work for traction of capsule 

itself. And present study is conducted to analyze the comprehensive structure of 

lens fiber configuration to induce increase of central thickness and decrease of 

vertical diameter in primate eyes. 

Morphology of zonule, capsule and lens fiber arrangement and tracer uptake 

through capsule were examined. 

Premature lens had horizontally aligned fiber and vascular net. The most 

distinctive feature in mature lens was intracapsular zonular branching in equatorial 

and anterior apical regions. Adult lens showed subepithelial elastic lamellae 

localized in superficial equatorial and anterior region. In aged lens major internal 

structure was horizontally arranged, tightly packed nonelastic fibers. 

Lifelong growth of lens fiber causes degenerative deterioration on fiber flexibility 

and also capsular responsibility to the zonular active traction. These finding may 

support our theory. 

OIPM 

CCP 

 

 

 

50 

 

 

connecting cilium 

CCP1 

 

 

 

CCP1 

3 

 

TTLL1 

CCP1 TTLL1 

CCP1 

CCP1 

CCP1

122 

117 

OIPM 

 

 

 

 

TRPtransient receptor 

potential 

 

 

 

TRPV 

ruthenium red TRPV4 

RN1734 ATP 

 

 

TRPV4

117 123 

P 

SA 

1 2 

1 

2 

Glial fibrillary acidic protein GFAP S100β 

 

 

 

S100 

S100A6 

 

 

S100A6 

CA1 S100A6 

CA1 

CA1 S100A6 

GFAPS100β 

S100A6 brain lipid binding protein BLBP 

S100A6 

 

 

P 

Distribution of corticosteroid receptors in oligodendrocytes of mice 

Yumiko Matsusue 1,2 , Noriko HoriiHayashi 2 , Takayo Sasagawa 2 , 

Wataru Matsunaga 2 , Katsuhiko Ono 3 , Mayumi Nishi 2 

1 

Dept. Oral. Maxillofac. Surg. Nara. Med. Univ., Nara, Japan, 2 Dept. Anat. & Cell 

Biol. Nara. Med. Univ., Nara, Japan, 3 Dept. Biol. Kyoto. Pref. Univ. Med., Kyoto, 

Japan 

Glucocorticoids are the mainstay in treating patients with diseases affecting the 

white matter, including multiple sclerosis in which the myelin sheaths around the 

axons of the brain, thereby leading to demyelination. In contrast, glucocorticoids 

are ineffective in gray matter injuries, such as head trauma. Many studies have 

reported glucocorticoid receptor GR is expressed in cultured oligodendrocytes. 

But very little study has revealed the expression of GR in oligodendrocytes 

in vivo. We investigated whether the oligodendorocytes were positive for GR 

by using two different oligodendorocyte markers, carbonic anhydrase CA 

II, a mature oligodendrocyte marker, and NG2, an oligodendrocyte progenitor 

marker. We focused on the gray matter regions including the cortex, hippocampus 

CA1, CA3, dentate gyrus, and amygdala, and the white matter regions 

including the external capsule, colpus callosum and fimbria hippocampus by 

using immunohistochemistry. We found over 80% of mature oligodendrocytes 

and oligodendrocyte progenitors express GR in various brain region. In 

contrast, neither oligodendrocytes nor oligodendrocyte progenitors express 

mineralocorticoid receptor MR. 

P 

F 

 

1 1 1 1 1 

2 2 3 

1 

2 3 

 

 

 

 

 

4F2 

9 

 

4F2 

4F2 

DEAD box RNA 

Ddx54 MBP 21.5 kDa 

 

 

P 

Immunohistochemical Analyses of Protein .G in Enteric Peripheral 

Nervous Tissues by in vivo Cryotechnique 

Jiaorong Chen, Nobuo Terada, Nobuhiko Ohno, Sei Saitoh, Yurika Saitoh, 

Shinichi Ohno 

Department of Anatomy and Molecular Histology, Interdisciplinary Graduate 

School of Medicine and Engineering, University of Yamanashi 

We have examined immunolocalization of protein 4.1G in peripheral nervous 

tissues of mouse large intestines with “in vivo cryotechnique” followed by freeze 

substitution. The 4.1G was mostly immunolocalized in Auerbach’s myenteric 

plexus. By immunofluorescence staining of 4.1G, GFAP and cKit, it was 

similarly immunolocalized with GFAP, but not with cKit. By preembedding 

immunoelectron microscopy, it was detected along glial cell membranes and their 

cytoplasmic processes around axon bundles. These findings indicate that 4.1G 

has some roles as adhesion and/or signal transduction in enteric glial cells of 

unmylinated nerve fibers in addition to myelination in the myelinated nerve ones. 

P 

 

in vivo in vitro 

1 1 2 1 

1 

1 2 2 

 

TJ 

TJ 

mesaxon, paranode, SchmidtLanterman 

autotypic tight junction 

TJ tricellulin TRIC, claudin19 cldn19, 

junctional adhesion moleculeC JAMC 

in vivo in vitro 

Myelin Protein Zero 13 P13 

TJ TRICCldn19 JAMC 714 P714 

Cldn19 JAMC 14 

TRIC 21 TRIC 

2 TJ 

4 Cldn19 JAMC 

mesaxonparanode SchmidtLanterman 

TRIC mesaxon paranode SchmidtLanterman 

TJ Cldn19 JAMC 

TRIC 

 

P 

 

 

1 2 2 1 2 

1 

1 

2 

 

10 

 

14 

 

MBP 

Calbindin 

 

 

Caspase3 

 

Cux1 

II/III, IV 

WGAHRP 

 

 

Laggard

124 

117 

P 

 

 

 

 

 

 

 

NeuN, PGP 9.5 

nestin, vimentin, S100, GFAP 

E15 

5 vCo5P0 3 vS32 

5 vL58 4 vL4 

3 

E15 vCo5 , P0 vCo4 2 

8 vCo2 

E15 vCo6 ,P0 

vCo8 2 8 vCo3 

 

 

 

P 

 

1 2 

1 

2 

 

490 

42 

21 

42 

 

 

4 10 

 

10 21 

21 

 

 

 

JSPS 23590223 

P 

 

 

 

 

 

 

 

 

 

 

 

Wistar 

Olfactory Marker Protein OMP 

 

OMP 

OMP 

 

 

 

 

 

 

P 

 

1 2 2 2 2 

2 2 

1 

2 

 

4-8 

 

 

 

 

 

 

8-12 

 

FG 

FG + FG 

DAB 

3D 

 

P 

 

DCC 

 

 

1 

 

1 

 

 

1 DCC deleted in 

colorectal cancer 

1 4 

DCC 

1 

DCC 

 

1 

DCC 

1 DCC 

* 

23590225 

P 

Spock3 null 

1 1 2 2 2 

1 

2 

 

Spock3 BM40/SPARC/osteonectin 

Spock3 

 

 

Spock3 

 

 

 

 

 

 

 

 

 

Spock3

117 125 

P 

 

γ 

 

 

 

 

 

 

4,52 PIP2 

4 5 PIP5Kγ 

PIP5KγK γ636γ661γ687 

RTPCR γ661 γ687 

 

PIP5Kγ 

 

PIP5Kγ 

 

 

KD 

PIP5Kγ 

636γ661γ687 KD 

PIP5Kγ 

661 

PIP5KγK 661 PIP2 

 

P 

 

1,2 1,2,3 

1 

2 

3 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

DNA 

 


 

P 

 

1,2 1,3 1 1,2 1,2 

1,2 1,4 1,2,3 

1 

2 3 

4 

V 

 

 

 

DiI 

 

 

 

 

 

 

 

 

 

RTPCR in situ 

hybridization 

 

P 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

DNA cDNA 

 

 

P 

 

 

 

 

 

 

 

hPAP 

 

LS2 

2 

 

II-III 

 

LS2 

hPAP T1 

hPAP 

LS2 ephrinA2 

Sema3F 

 

 

P 

W NPW 

 

1 1 2 1 

1 

2 

W G 

NPW 

 

 

NPW Y 

 

NPW 

DIO 

NPW 

NPW 

cFos NPW 

DIO 

NPW NPW 

cFos 

NPW 1

126 

117 

P 

Chewing under restraint stress inhibits the stressinduced 

suppression of cell proliferation in the hippocampal dentate gyrus 

Kinya Kubo 1 , Nobuyuki Karasawa 1 , Kazuhiko Satoh 2 , Yasutoku Kogaya 2 , 

Sadakazu Ejiri 2 , Huayue Chen 3 

1 

Seijoh Univ Grad Sch Health Care Studies, 2 Dept Oral Anat, Asahi Univ Sch 

Dent, 3 Dept Anat, Gifu Univ Grad Sch Med 

Stress reduces new cell birth in the hippocampus dentate gyrus DG. Chewing 

under stress suppresses stressinduced responses. We examined whether chewing 

under restraint stress prevents the stressinduced suppression of cell proliferation. 

Male SAMP8 mice were used and divided into three groups as follows: control, 

restraint stress group, and restraint/chewing group. Mice in the restraint stress 

and restraint/chewing groups were placed in a ventilated plastic restraint tube, 

and then the tube containing the mouse was placed under a bright light for 45 

min, once a day for 14 days. Mice in the restraint/chewing group were allowed 

to chew on a wooden stick during this period. Plasma corticosterone levels were 

significantly higher in the restraint stress group than in the control and restraint/ 

chewing groups in association with decreased cell proliferation in the hippocampal 

DG. The increase in plasma corticosterone levels induced by restraint stress was 

inhibited in the restraint/chewing group, and the reduction in cell proliferation was 

attenuated. These findings suggest that chewing under restraint stress prevents the 

stressinduced suppression of cell birth in the hippocampal DG. 

P 

Voluntary exercise promotes astrogliogenesis from Olig cells in 

some nuclei of the basal ganglia of adult mouse 

Kouko Tatsumi 1 , Hiroaki Okuda 1 , Mariko Yamano 2 , Akio Wanaka 1 

1 

Department of Anatomy and Neuroscience, Nara Medical University, 

2 

Department of Comprehensive Rehabilitation, Osaka Prefectural University 

We have previouly reported that astrocytes derived from Olig2 cells dramatically 

increased in the subthalamic nucleus STN after voluntary exercise in adult mice. 

Recently we comfirmed that astrogliogenesis is promoted in not only STN but also 

another nuclei of basal ganglia, e.g., the globus pallidus GP, substantial nigra 

SN, by voluntary excercise. Additionally, we observed that cfos expression 

significantly increased in neuron of these nuclei. It is well known that tha basal 

ganglia regurate motor activity by processing descending information from 

cortical regions. These findings implied the neuronastrocyte relationship based on 

glutamate metabolism in response to exercisestimuli. To confirm this relationship, 

we used fluorocitrate FC to metabolically inhibit astrocyte functions. As would 

be expected, mice regionally deficient of astrocytes in the STN exhibited reduction 

of spontaneous activities. On the other hand, FCmicroinjection into other but 

neighboring regions had little influence on motor activities. These findings suggest 

that astrocytic activities of the STN are closely related to neuronal outputs of the 

STN, possibly through the glutamate metabolism. 

P 

GDNF mRNA 

1 2 1 3 1 

1 

2 3 

Glial cell linederived neurotrophic factor GDNF 

 

Wistar 2 

12 10 10 4 

17 m/min, 1 

PCR GDNFGfra1Gfra2mRNA 

GDNFmRNAGfra2mRNA 

Gfra1mRNA 

 

 

P 

II 

 

 

 

 

IGFVEGF 

 

 

IIAngII 

 

AngII 

 

AngII ELISA 

2 

AngII 

1 1 8 Bromodeoxiuridin 

BrdUVehicle BrdU 1.5 

I 

AngII VEGF 

ELISA AngII 

 

P 

 

1 1 2 3 1 2 

2 1 2 

1 

2 3 

 

 

 

 

 

 

 

Watson 

2 Exercise 

EX Control CNT 

246810 Rotarod test 

HPLC 

 

Rotarod test EX 

6 CNT 

2 

DA 1.7 10 EX 

DA CNT 

CNT TH 

 

P 

cfos 

 

1 2 1 1 1 3 

3 4 

1 

2 

3 

4 

 

 

 

 

cfos C57BL 

4 , 

20 12 m/min5 

5 / 4 

 

cfos 

cfos 

34 cfos 

22cfos 

 

 

cfos

117 127 

P 

cFos 

 

1 2 1 1 1 3 

3 4 

1 

2 3 

4 

 

 

 

 

cFos 5 

C57BL High fat diet 32 4 

2 

5 12 m 20 

5 4 

5 μm ABC 

cFos 

cFos 

 

 

 

cFos 

 

 

P 

 

1 1 1 2 1 

1 

2 

 

 

 

 

 

 

 

 

GFP 

E14 

GFP 24h 

Tbr2 

 

 

Tbr2 

radial glia 

 

P 

 

 

 

 

 

 

 

 

 

SD 

2-20 6 / 

5HT 

5HT 

Egr1 

 

 

Egr1 

21 

 

 

 

 

 

P 

/ 

 

 

 

 

 

 

 

 

Wistar 1015 3 

/ IGF1/IGF1R BDNF/TrkB 

1620 30 60 

 

 

 

/ 

 

P 

n 

 

 

 

 

1 

 

 

n3 

 

21 

 

 

 

 

 

 

 

 

 

 

P 

Altered dynamics of the cortical neuronal circuit in a mouse model of 

autism 

Shinji Tanaka 1 , Toru Takumi 2 , Shigeo Okabe 1 

1 

Department of Cellular Neurobiology, Graduate School of Medicine, University 

of Tokyo, 2 Laboratory of Integrative Bioscience, Graduate School of Biomedical 

Sciences, Hiroshima University 

Autism spectral disorder ASD is an early onset mental disease with impairments 

in higher cognitive functions. ASD symptoms may originate from altered 

connectivity of the neocortical neurons. This possibility can be tested by 

combining techniques of synapse imaging with reliable animal models of ASD. 

Genetically engineered mice that mimic the most frequent copy number variation 

in ASD human 15q1113 show multiple deficits in social behaviors. The 

postnatal synapse formation in the neocortex of this ASD model was studied by 

using twophoton in vivo imaging of pyramidal neurons expressing fluorescent 

proteins. Pyramidal neurons showed excess formation of nascent spines and 

their balanced elimination. Unexpectedly, analysis of PSD size changes in 

established spines, estimated from PSD95 clustering, revealed reduced PSD 

remodeling. Enhanced gain and loss of spines during network formation may 

increase mismatched connectivity, leading to altered pattern of neuronal activity 

that secondarily suppresses PSD remodeling. Negative correlation between 

spinogenesis and PSD remodeling may be a unique feature of this mouse model 

and its relevance to the etiology of ASD should be clarified.

128 

117 

P 

Mechanism of deficit in aggressive behavior of metabotropic 

glutamate receptor subtype knockout mice 

Miwako MasugiTokita 1 , Peter Josef Flor 2 , Mitsuhiro Kawata 1 

1 

Dept. of Anatomy and Neurobiology, Kyoto Pref. Univ. of Med., 

2 

Univ. of Regensburg, Regensburg, Germany 

Metabotropic glutamate receptors mGluRs consist of eight different subtypes 

and exert their effects on second messengers and ion channels via Gproteins. 

The function of individual mGluR subtypes in the CNS, however, largely remains 

to be clarified. We examined the aggressive behavior in male wildtype and 

mGluR7 knockout littermates, using a residentintruder paradigm. Wildtype 

mice displayed intense aggression against olfactory bulbectomized intruders. 

In comparison, mGluR7 knockout mice showed significantly reduced levels 

of aggression. We also found that mGluR7 knockout mice showed grooming 

behavior against intruders. 

Olfaction is known to be the essential components of aggressive behavior. 

Although mGluR7 is widely distributed in the brain, intense expression is found in 

olfactory system. To investigate the mechanism of altered aggression, we further 

assessed urine preference of mGluR7 knockout mice. Two cotton pads wetted 

with different odorant sources were presented simultaneously to the mice. Wild 

type mice spent longer time to sniff male urine than saline, but mGluR7 knockout 

did not show such preference. 

P 

PTPRA 

 

 

PTPRA 

 

PTPRA 

 

PTPRA 

 

PTPRA 

9 

 

 

PTPRA 

 

 

P 

Kisspeptin 

 

 

Kisspeptin 

 

kisspeptin 

 

kisspeptin 

kisspeptin in situ hybridization 

7 kisspeptin 

 

kisspeptin 

18 kisspeptin 

kisspeptin 

 

9 

kisspeptin 

kisspeptin 

 

 

P 

 

 

 

 

GnRH 

 

 

GnRH 

 

 

21 

30 42 60 Wistar Rat GFAP 

GFAP 

 

GFAP 21 

30 

GFAP 

 

GFAP 

 

GnRH 

 

 

P 

 

 

 

 

 

GiPAG 

 

 

 

 

Gi PAG 

 

GFAP 

Gi PAG GFAP 

Gi α GiA GFAP 

 

α Gi PAG 

GiA 

GFAP 

GiA 

 

P 

TGFβ 

 

 

 

 

TGFβ1 

transforming growth factorβ1 TGFβ1 Komuta 

et al., Cell. Mol. Neurobiol. 30: 101111, 2010 10 

 

 

 

 

14 

TGFβ1 

 

0 

TGFβ1 

 

TGFβ1

117 129 

P 

Identification of CSPG constituting DACS, a novel brain extracellular 

matrix 

Hiroaki Okuda 1 , Yukinao Shibukawa 2 , Hiroaki Korekane 3,4 , 

Noriko HoriiHayashi 5 , Kouko Tatsumi 1 , Yoshinao Wada 2 , 

Naoyuki Taniguchi 3,4 , Akio Wanaka 1 

1 

Dept. Anatomy and Neuroscience, Nara Med. Univ., 2 Dept. Molecular Medicine, 

Osaka Med. Center and Research Institute for Maternal and Child Health, 

3 

Dept. Disease Glycomics, RIKENISIR, Osaka Univ. Alliance Lab., 4 Systems 

Glycobiology Research Group, Chemical Biology Dept., Advanced Science 

Institute, RIKEN, 5 Dept. Anatomy and Cell Biology, Nara Med. Univ. 

An antichondroitin sulfate antibody CS56 delineated a structure with a 

unique morphology like a dandelion clock. We named it DAndelion Clock 

like Structure DACS BBRC; 364: 4105, 2008. DACSs surrounded a group 

of NeuNpositive/GABAnegative neurons. At an ultrastructural level, CS56 

immunoreactivities were localized in the cytoplasm and on the membrane of 

astrocytes. As the postnatal cerebral cortex matured, DACSs became visible 

from the end of the critical period. Furthermore, DACSs is conserved among 

species. In this study, we have identified the core protein of DACSconstituting 

CSPG. We purified core proteins from the mouse cerebral cortex by anion 

charge, charge transfer and size exclusion chromatographies and identified one 

of them as TenascinR TNR using mass spectrophotometry. TNR is thought to 

constitute socalled perineuronal net that modulates plasticity of interneurons. In 

situ hybridization analysis revealed that TNR mRNA was selectively localized 

at CS56positive astrocytes, but not WFApositive GABAergic interneurons 

in the adult mouse brain. These results suggest that TNR may play previously 

unexpected roles in a subpopulation of cortical astrocytes. 

P 

 

1 2 1 

1 

2 

 

 

 

 

 

1980 

 

GABA 

 

barrel 

 

 

 

 

 

 

 

 

 

P 

Mutations in POLRA and POLRB encoding RNA polymerase III 

subunits cause an hypomyelinating leukoencephalopathy 

1 2 3 4 5 

4 1 1 

1 

2 3 

4 5 

 

We have recently reported a hypomyelinating syndrome characterized by diffuse 

cerebral hypomyelination with cerebellar atrophy and hypoplasia of the corpus 

callosum HCAHC. We performed whole exome sequencing of three unrelated 

individuals with HCAHC, and identified compound heterozygous mutations in 

POLR3B in two individuals. The mutations include a nonsense mutation, a splice 

site mutation and two missense mutations at evolutionally conserved amino acids. 

Using reverse transcriptionPCR and sequencing, we demonstrated that the splice 

site mutation caused deletion of exon 18 from POLR3B mRNA, and that the 

transcript harboring the nonsense mutation underwent nonsensemediated mRNA 

decay. We also identified compound heterozygous missense mutations in POLR3A 

in the remaining individual. POLR3A and POLR3B encode the largest and second 

largest subunits of RNA Polymerase III Pol III, RPC1 and RPC2, respectively. 

Pol III is involved in the transcription of small noncoding RNAs, such as 5S 

ribosomal RNA and all transfer RNAs tRNA. Perturbation of Pol III target 

transcription could be a common pathological mechanism underlying POLR3A 

and POLR3B mutations. 

P 

 

1 1 1 1 1 2 

2 

1 

2 

polyQ 

CAG 

 

polyQ 

 

 

polyQ 

ataxin3 polyQ Q77trAT3 

polyQ ubiquitin 

polyQ 

islet1 

 

PolyQ 

1TUNEL 

caspase3 2 Bclxl 

 

 

P 

Zitter rat 

 

 

Zitter attractin 

 

 

Zitter 

 

 

 

 

Zitter 

 

 

 

2 

 

 

 

 

 

P 

 

1 2 2,3 4 4 2 

1 

2 3 

4 

 

 

Marchi Nauta 

 

 

Weigert Luxol 

fast blue 

 

 

20 5 

Goto 1987 LPH 

 

100

130 

117 

P 

Stigmoid body 

Islam Md. NabiulJahan Mir Rubayet 

 

 

Stigmoid body STB 

HAP1huntigtin associated protein 1 

STB HAP1 

 

 

HeLa wistar PCM1 pericentriol 

material 1 

 

PCM1 

diffuse 

inclusion 3 2 PCM1 

PCM1 inclusion STB 

HAP1 

PCM1 HAP1/STB STB 

STB HAP1 PCM1 90% 

PCM1 inclusion STB 

in vitro HAP1 

HeLa STB PCM1 

HeLa STB PCM1 

 

P 

 

1 1 2 1 

1 

2 

 

 

Holmes 

 

PTAH 

 

 

 

 

ddY 20 g 

6 μm 

PTAH 

 

 

PTAH 

 

 

 

Holmes 

 

P 

 

1,2 1,3 2 1 

1 

2 3 

 

 

 

91 

 

celloidin Kultschitzky 

 

 

90 1 1 

celloidin 

 

 

2 

 

P 

Eusthenopteron foodi 

 

1 2 3 4 

1 

2 3 

4 

Eusthenopteron foodi 

Eusthenopteron foodi 

TEM 

SEMEPMA X 

 

TEM 

 

2 

 

TEM 

X 

fluoraptite EPMA 

Ca/P 1.96 Ca/P 

1.87 F 1.87-5.11Wt 

2.10-4.55 Wt 965- 

967cm 1 

 

P 

 

 

 

 

 

 

 

 

 

 

 

Palaeoniscus 

 

3 

Palaeoniscus 

 

 

 

 

P 

 

 

 

 

 

 

 

 

 

11 

14 4 

 

11 12 

14 

 

12 

14 

13 

14

117 131 

P 

NOS 

1 2 4 4 4 

3 4 

1 

2 

3 

4 

NO 

 

NO 

NO 

 

NOS 

0371014 C57BL/6J 

4 PA 

10%EDTA 

nNOSiNOSeNOS 

 

NOS 

07 nNOS i 

eNOS 1014 nNOS 

 

ieNOS 

10 NOS 

 

 

P 

 

1 1,2 1 1 1 

1 

1 

2 

1AQP1 

 

AQP1 

 

AQP1 

2 

AQP1 

 

- 

---- 

-- 

in situ AQP1mRNA 

 

 

AQP1 

 

AQP1 

 

P 

TNFα/RANKL microRNA 

 

 

microRNA 21 nt noncoding RNA mRNA 

3’UTR 

 

RANKL microRNA 

 

TNFα 

microRNA 

 

RAW264.7 

TNFα TNFα RANKL 

RANKL 10 ng/ml 

TNFα/RANKL 

0, 24, 82 total RNA 

microRNA qRTPCR 

 

TNFα/RANKL 2 microRNA 

44 miR1224 

miR223 

microRNA TNFα/RANKL 

 

P 

hemokinin 

1 2 1 1 1 

1 2 

1 

2 

1HK1 

1 NK1 P SP SP 

HK1 

 

in vivo 5 

HK1 

in vitro RANKL 

MCSF 7 RTPCR 

HK1 NK1 

SP HK1 TRAP 

 

in vivo HK1 

 

in vitro HK1 NK1 

TRAP 

SP HK1 

SP HK1 SP 

SP 

HK1 

HK1 NK1 SP 

 

P 

DGKζ Retinoblastoma 

1,2 2 1 

1 

2 

DGK 

 

Retinoblastoma Rb E2F 

DGK 

DGKζ Rb C2C12 

DGKζ Rb 

 

DGKζ 

DGKζ 

Rb 

ATDC5 

DGKζ DGKζ 

Rb 

DGKζ mRNA 

 

P 

 

1 1 1 1 2 

1 

1 

2 

 

SSEA3 

Multilineagedifferentiating 

Stress Enduring Muse Muse 

 

 

 

 

 

 

Muse 

FACS SSEA3 

 

1 

6 

 

Muse

132 

117 

P 

PACAP VIP 

PACAP VIP 

1 2 2 1 

1 

2 1 

 

PACAP 

 

VIP 

PACAP 

8 C57BL/6 

PACAP PAC1R VIP 

VPAC1 PAC1R 

pillar 

cell 

VPAC1 

pillar cell 

PAC1R 

VPAC1 

PACAP VIP 

 

 

 

P 

 

 

1,2 2 2 1 2 

2 

1 

2 

5 AQP5 

AQP5 

AQP5 

AQP5 

 

9 Wistar 

AQP5 

3 AQP5 

 

 

 

AQP5 

 

AQP5 

 

P 

gustducin 

 

2 

116 

gustducin G 

NSE 

 

 

4 Hartley 4%PFA 

 

calbindin gustducin 

ABC gustducin 

TEM 

0.5-1 μm 

ABC gustducin 

 

gustducin calbindin TEM 

 

 

gustducin 

 

P 

 

 

 

 

 

ZnT 

 

ZnT7 ZnT5 

ZnT2 ZnT5 Se Zn 

 

 

ZnSO45 mg/100 g ZnT 

 

 

 

H1250M 

 

 

 

 

 

P 

 

1 1 1 2 1 

2 1 

1 

2 

P 

APC Min/+ 

 

1 1 1 2 

1 

2 

HSC 

 

E HSC 

δ 

HSC 

HSCT6 δ 

HepG2 

α 

HepG2 

 

4 δ 

FACS TUNEL δ 

HSC 

 

δ 

β1 

 

 

APC Min/+ 

 

Interstitial cells of 

Cajal; ICCICC 

 

 

ICC 

7 APC Min/+ 

ICC 

ICCMPICCDMP 

cKit 

cKit ICCMP 

ICC 

 

 

ICC ICC

117 133 

P 

DPEP 

1 2 2 1 2 

1 

2 

Dipeptidase1 DPEP1 dipeptideglutathioneleukotrieneD4 

 

GPI 

DPEP1 

HCC56 

 

caveolin1 flotillin1 

GPI CD59 

 

DPEP1 

GPI 

 

 

P 

Placenta specific miR-517a modulates gene expression in Jurkat 

cells 

Md Moksed Ali 1 , Xiaohui Song 1,2 , Osamu Ishibashi 1 , Kunio Kikuchi 1 , 

Tomoko Ishikawa 1 , Takami Takizawa 1 , Toshihiro Takizawa 1 

1 

Dept Mol Med & Anat, Nippon Medical School 2 Dept Pharm, Harbin Medical 

University 

[Objective] MicroRNAs miRNAs are noncoding RNAs that repress gene 

expression posttranscriptionally. Recently we have found that human placenta 

specific miRNAs are transferable to Jurkat cells human T cell lymphoblast 

like cell line via exosomes manuscript in preparation. In this study, we 

overexpressed placenta specific miR-517a in Jurkat cells and did microarray 

analysis MA to identify its target mRNAs. 

[Methods] MA was performed on Jurkat cells overexpressing miR-517a. 

Validation of genes downregulated by miR-517a was carried out by realtime 

PCR, Western blot, and 3'UTRluciferase reporter assay. 

[Results] We found that cyclic GMP dependent protein kinase 1 (PRKG1), one 

of 8 downregulated genes identified in MA, is a target of miR-517a in silico. MiR- 

517a significantly downregulated the expression of PRKG1 at both the mRNA and 

protein levels. Furthermore, miR-517a significantly decreased luciferase reporter 

activity. 

[Conclusion] We identified that PRKG1 is indeed a target of miR-517a in Jurkat 

cells. These findings provide a mechanistic insight on the posttranscriptional 

regulation by miRNAs of placentaderived exosomes in T cells. 

P 

microRNA CpG 

1 1,2 3 1 

1 

2 3 

 

microRNA miRNA 22 RNA 

 

19 miRNAs C19MC; miR-517a 

Luo 

et al. Biol Reprod 81: 717729, 2009 18 

kb CG DNA 

C19MC NoguerDance et al. 

Hum Mol Genet 19: 35663582, 2010 

BeWo JEG3 

HTR8/SVneo C19MC 

miRNA PCR 

DNA PCR 

CG 

BeWoJEG3 C19MC HTR8/SVneo 

BeWo, JEG3 C19MC 

HTR8/SVneo 

 

P 

IgG IIb Fc 

FcRIIb 

1 2 1 3 1 

1 

2 3 

 

HPEC 

IgG 

HPEC IIb Fc 

FcRIIb IgG 

J Immunol 175: 2331, 2005 

FcRIIb RAB GTPasesRAB1B RAB3D 

FcRIIb in vitro FcRIIb 

GFP FcRIIb 

pFCGR2B-GFP HUVEC 

FcRIIb RAB1BRAB3D siRNA 

FcRIIbsiRAB1B 

siRAB3D HUVEC pFCGR2B-GFP 

GFPFcRIIb GFPFcRIIb 

HUVEC siRNA GFPFcRIIb 

GFPFcRIIbHPEC 

RAB1B RAB3D FcRIIb 

 

P 

Agerelated accumulation of nonheme ferric and ferrous iron in 

mouse ovarian stroma visualized by sensitive iron histochemistries 

Yoshiya Asano 

Department of Neuroanatomy, Cell Biology and Histology, Hirosaki University 

Graduate School of Medicine 

Sensitive nonheme iron histochemistries, namely perfusionPerls and Turnbull 

method, were applied to study the distribution and agerelated accumulation of 

nonheme iron in mouse ovary. Microscopic studies revealed that nonheme ferric 

iron is distributed predominantly in stromal tissue, especially in macrophages. By 

contrast, the distribution of nonheme ferrous iron was restricted to a few ovoid 

macrophages. Aged ovaries exhibited remarkable nonheme iron accumulation in 

all stromal cells. Particularly, nonheme ferrous iron level was increased in stroma, 

suggestive of increased levels of redoxactive iron, which can promote oxidative 

stress. Moreover, intense localization of both nonheme ferric and ferrous iron was 

observed in aggregated large stromal cells that were then characterized as ceroid 

laden enlarged macrophages. Iron overload in adult mice resulted in nonheme 

iron deposition in the stroma and generation of enlarged macrophages, suggesting 

that iron accumulation induced morphological changes. Our data indicated that 

nonheme iron accumulation in stromal tissue may be related to aging of the ovary 

which due to the increasing of oxidative stress. 

P 

 

 

 

 

 

CD34 

CD141 

 

 

 

5 μm CD34 CD141 DAB 

CCD 

 

 

 

CD34 

CD141 

CD34 

 

 

CD34

134 

117 

P 

 

 

1 1 2 2 1 

1 

2 

 

 

 

 

 

 

 

42.3 

M 1 μm 

 

 

10.1 

48 

 

 

 

1 1 

GTH 

 

P 

TG 

1 2 2 1 1 2 

1 

2 

 

TG tdTomato 

tdTomato 

 

in vivo 

 

4%PFA 

10 μm filmtransfer 

 

 

 

 

 

 

 

 

 

P 

 

1 2 1 1 1 

1 1 1 1 3 2 

1 1 

1 

2 

3 

 

X CTMRI 

CT 

1 

90 

CT 

 

 

 

 

 

 

 

 

 

 

 

P 

 

Dolgorsuren AldartsogtShineOd Dalkhsuren 

 

 

 

 

 

 

31 49 

3 

 

HE 

21 

26 2 

 

21 5 

16 26 

13 13 

 

 

 

 

 

 

P 

 

1 1 1 1 2 

1 

1 

2 

 

 

Nikolai and Bramble, 1983 

 

2 

Dipodomys merriami 

Jaculus orientalis 

Rattus rattus Satoh and Iwaku, 

2008 

 

13 

 

 

 

2 

 

 

P 

 

1 1,2 1 

1 

2 

2 

 

 

80 

 

 

 

 

12 Wistar 1

117 135 

P 

Activities of human upper limb muscles during a combined 

movement of elbow flexion/extension and forearm pronation/ 

supination 

Makoto Naganuma 1 , Wataru Hashizume 1 , Katsuhiko Suzuki 2 , Toshiaki Sato 3 , 

Hiromi Fujii 3 , Akira Naito 1 

1 

Dept Anat, Yamagata Univ Sch Med, 2 Dept PT, Yamagata Pref Univ Health Sci, 

3 

Dept OT, Yamagata Pref Univ Health Sci 

Facilitation and inhibition mediated by group I afferents among human upper 

limb muscles have been studied. The former and latter must be active during, 

respectively, cocontraction and reciprocal contraction of the muscles. This study 

examined activities of the biceps brachii BB, pronator teres PT, extensor carpi 

radialis ECR and ulnaris ECU, and flexor carpi radialis FCR and ulnaris 

FCU during a combined movement of elbow flexion/extension EF/EE and 

forearm pronation/supination FP/FS in ten normal men with electromyography. 

During movements of EFFP/EEFS and EFFS/EEFP, BB and FCU group 

BB showed parallel activities cocontraction increasing and decreasing and 

PT, ECR, and ECU group PT those decreasing and increasing at the EEFS and 

EFFS, and EFFP and EEFP phases, respectively. These fluctuations resulted 

in reciprocal contraction between group BB and PT. The results suggest that the 

facilitation between PT and ECR and the inhibition between BB and PT are active 

during the movements. Findings of the contractions may indicate existence of 

facilitation between PT and ECU, and ECR and ECU, and inhibition between PT 

and FCU, ECR and FCU, and ECU and FCU. 

P 

 

1 3 2 2 2 

2 

1 

2 3 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

P 

 

P 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

nerve point 

 

23 cm 

Erb’s point 

P 

 

1 2 3 2 2 

1 

2 3 

 

 

31 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

P 

 

1 2 3 4 5 5 

5 

1 

2 3 

4 5 

 

2010 8 4 

82 

 

5 cm 1 cm 

5 mm 1 

2C7C8 3C7 

4 1 

13C7 

4 2 

 

3 

2 

 

 

C6 1/3

136 

117 

P 

 

1 2 2 

1 

2 

3 1 C3T1 

201121:1 

 

 

 

C36C47 

C6T1 C57 

C5,6 C35C58 

 

M. coracohumeralis C5,6 

 

M. coraco 

humeralis 

 

 

 

 

 

P 

 

1 2 2 3 4 

4 4 

1 

2 3 4 

 

 

 

 

20 50 25 15 10 

2 cm 

100 

Prick painDull pain 

100 

25 

 

 

 

 

 

 

 

 

 

P 

 

 

1,2 2 3 2 4 1 

1 1 

1 

2 3 

4 

 

 

 

MP MP 

13 26 

21 12 16 

MP 

MP 

 

MP 

 

 

MP Levene 2 

MP 

MP MP 

 

MP MP 

 

P 

 

 

 

 

 

 

 

 

22 

582 1088 567 1125 

 

723 584 

584 

440 118 

26 

139 74 

64 

 

 

 

 

 

P 

 

1 1 2 1 1 1 

1 

1 

2 

 

2011 

 

84 

 

 

 

2 

 

 

6 

 

 

 

 

 

Adachi G 

P 

 

1,2 2 2 

1 

2 

 

6 7 

7 

SS 3 SS+BS 

2 N 2 1 

SpsCs 

SS SS+BS N Sps 

2 Cs SS 1 

SS+BS 2 Sps 3 

SpsCsTd 3 RmA 

RmS RmBN 

RmA RmS Td SS 

RmS SS+BS RmS Sps 

RmB 2 

Sps Cs 

 

SS SS+Bs Sps Cs

117 137 

P 

 

 

 

2010 77 

 

 

2 

 

 

 

1 

 

 

2 

3 

 

4 

 

5 

 

P 

 

1 1,2 3 1 1 3 

1 

2 

3 

 

 

 

 

2006 

 

 

 

2010 37 

21.6837 

 

 

5.4237 

 

 

2011 

 

P 

Persistent left hepatic venous directly opening into the right atrium 

1 2 2 1 

1 

2 Department of Anatomy, Faculty of 

Medicine, Chiang Mai University 

An anomalous left hepatic vein opening independently of the coronary sinus into 

the right atrium was found in the cadaver of an 88yearold Japanese man. This 

vein originated from the left lobe of the liver, perforated the diaphragm at the left 

side of the vena caval foramen and opened into the right atrium. The left hepatic 

vein anastomosed mutually with the middle hepatic vein at the level of venule. 

The ligamentum venosum originated from the left branch of the portal vein and 

was connected directly to the left hepatic vein. The development of the central 

systemic venous system and a possible explanation for the morphogenesis of this 

anomaly were reviewed. As a result, the occurrence of this anomalous vein was 

explained as being due to the persistence of the left vitelline connection with the 

left sinus horn and the ductus venosus. 

P 

 

 

I 

G L H 

M 3 TC 

 

4 G, L, H, M 

4 

1. TC H HA 2. 

AA HA 3. TC M HA 

4. M H HA 5. M H HA 

6. TC M AA 

H 

M 5 

 

 

TC 

 

 

P 

 

1 1 1 1 1 2 

1 

1 

2 

30 2 

3 4 20 21 

 

7 8 

1 1 1 85 

5 3 

hilar artery 

1 2 

78 4 4 1 

 

2 2 3 2 

hilar artery 2 1 

polar artery 

hilar artery polar artery 

 

rete arteriosum Felix 

 

P 

 

1 1 1 2 1 

1 

2 

 

 

90 

30 510 

mm

138 

117 

P 

 

 

 

 

 

622 366 

453 

 

77.8%35/45 

71.2%37/52 70.3%71/101 87.7%71/81 

83.8%57/68 84.2%16/19 

89.5%51/57 94.3%66/70 85.3% 

145/170 95.8%68/71 93.5%58/62 91.3% 

21/23Fisher 

5% p=0.046 

 

 

 

 

P 

 

1 1 1 2 3 

3 3 3 

1 

2 

3 

 

 

 

 

 

2,000 

 

 

 

 

 

 

 

 

 

 

 

P 

 

1 2 3 4 5 

6 

1 

2 

3 

4 5 

6 

 

40 

 

 

 

 

 

P 

LEH 

1 1 2 3 1 

1 

2 

3 

 

 

 

1710 

 

 

 

 

LEH 

LEH 

 

 

 

 

 

 

 

 

 

P 

 

1 2 1 

1 

2 

 

Phenice 

 

Bruzek Murail 

Phenice Bruzek 

 

 

Phenice 

90Bruzek 9294Murail 7580Bruzek 

 

8994 8694 

PheniceBruzekMurail 

 

P 

 

 

1 1 1 1 2 

3 4 5 

1 

2 3 

4 5 

 

205 83 

35 

 

P0.05 

2 

 

45.6% 40.3% 

12.9% 14.9% 1.2% 2.3% 

15 

17 Smith 

 

 

 

 

Smith

117 139 

P 

Nacholapithecus kerioi 

 

1 2 3 3 4 5 

6 4 7 

1 

2 3 4 

5 6 7 

Nacholapithecus kerioi 1500 

1999 2002 

Nacholapithecus 

Papio cynocephalus 

 

 


 

 

 

 

 

MacLarnon1995intermembral 

index 

 

 


#20247033 

P 

Involvement of Olig in the formation of reciprocal connection 

between the thalamus and cortex 

1,6 C Parras 2 Q Zhang 3 4,6 5 

6 

1 

2 Institute of the Brain and Spinal Cord ICM, Inserm 

UPMC 3 BSI 4 5 

6 

Olig2 is a transcription factor essential for oligodendrocyte and motor neuron 

generation in the spinal cord. Although Olig2 is expressed throughout the 

embryonic central nervous system, the role of Olig2 in the brain development 

has not been uncovered well yet. To explore new function of Olig2, we first 

analyzed axonal architecture in Olig2 deficient mice. Immunohistochemistry 

against neurofilamentM showed abnormal course and fasciculation of axons 

in the mutant thalamus. Expression analysis of NetrinG confirmed that both 

thalamocortical and corticothalamic fibers are impaired in the mutant. We next 

performed microarray analysis of Olig2 deficient basal forebrain, and identified 

that several axon guidance molecules were up or downregulated in the Olig2 

mutant. To examine spatiotemporal expression patterns of these genes, we carried 

out in situ hybridization, and revealed that the expression of EphA3, Robo1 and 

Slit2 were altered in the mutant diencephalon. Our results suggest that Olig2 plays 

an essential role in the formation of reciprocal connection between the thalamus 

and cortex via regulating the expression of several axon guidance molecules. 

P 

Studying nascent daughter cells’ neighborship to understand the 

mechanism of cell fate choices in the neocortical neurogenesis 

 

 

Cellcell interactions are very important for developmental events that construct 

the central nervous system. However, we still cannot tell exactly when and 

where these interactions occur for nascent daughter cells born at the apical/ 

ventricular surface of the neuroepithelium NE or ventricular zone VZ. NE/VZ 

is heterogeneous with cells differing in differentiation state and cell cycle phase, 

allowing cells to “neighbor” make contacts to a variety of types of cells. It is 

possible that such “neighborship” dynamically changes for each cell, because 

cell movements are an active. To ask whether/how the history of neighborship 

for each daughter cell might affect its subsequent fate determination, we are 

comprehensively monitoring the composition of cells that surround a given 

nascent 3hrold daughter cell. Confocal microscopy to obtain optical slices 

parallel to the apical/ventricular surface using brain/retinal primordia prepared 

from ROSA26 Lynvenus mice allows us to observe outlines of all cells in the 

periventricular area. Our live observations suggest that daughter cells born 

almost simultaneously within a limited area can be different as to how they are 

surrounded. 

P 

GABAA 

1 2 1 1 

1 

2 

Student Lab 

GABAA 

 

Tochitani et al., 2010 GABAA 

 

10 E10 

GABAA alpha4 

E10 E12 GABA 

E14 

GABA 

GABA GAD65/67 

GABA GABAA 

Taurine E10 

preplate E12 E14 subplate marginal zone 

GABAA 

 

intermediate progenitor 

GABAA 

intermediate progenitor 

P 

 

 

 

 

 

 

6 

 

 

transplantation 

 

 

 

transplantation 

14 

 

 

 

 

P 

kirrel 

1 1 1 2 1 

1 

1 

2 

 

A member of the immunoglobulin superfamily, kirrel3 is widely expressed in 

the developing and adult central nervous system CNS. In the present study, 

we investigated spatiotemporal expression of kirrel3 in the CNS using kirrel3 

lacZ knockin mice. The expression of βgalactosidase βgal was found in 

the various regions of developing and adult CNS, including the olfactory bulb, 

striatum, cerebral cortex, hippocampus, and cerebellum. In the adult olfactory 

bulb, the expression of βgal was observed in the calbindin or carlretininpositive 

periglomerular neurons, subpopulation of Reelinpositive external tufted cells 

and mitral cells, and GAD67positive granule cells. In the adult striatum, βgal 

positive cells were mainly observed in the ventral striatum. In the developing 

olfactory bulb and striatum, βgal was expressed in the differentiating zone, but 

not in the ventricular zone. These results suggest that kirrel3 may be involved in 

development of several neural circuits, including the olfactory system and limbic 

system. 

This work was supported by a GrantinAid for Scientific Research B from 

Japan Society for the Promotion of Science 22390036.

140 

117 

P 

Characterization of the RNAbinding protein Musashi in zebrafish 

 

 

Musashi Msi is an evolutionarily conserved gene family of RNAbinding 

proteins RBPs that is preferentially expressed in the nervous system. The first 

member of the Msi family was identified in Drosophila. Drosophila Msi plays 

an important role in regulating asymmetric cell division of the sensory organ 

precursor cells. The mammalian orthologs, including human and mouse Msi1, are 

neural RBPs that are strongly expressed in neural stem cells NSCs. Mammalian 

Msi1 contributes to self renewal of NSCs through translational regulation of 

several target mRNAs. In this study, the zebrafish Msi ortholog zMsi1 was 

identified and characterized. The normal spatial and temporal expression profiles 

for both protein and mRNA were determined. Overall, zMsi1 was strongly 

expressed in neural tissue in early stages of development and exhibited similarity 

to mammalian Msi1 expression patterns. To reveal the in vivo function of zMsi1, 

morpholinos against Msi1 were introduced into onecell stage zebrafish embryos. 

Knock down of zmsi1 frequently resulted in aberrant formation of the CNS. These 

results suggest that Msi1 plays roles in CNS development in vertebrates. 

P 

PSANCAM 

1 2 1 

1 

2 

 

PSANCAMpolysialic acidneural cell adhesion molecule 

 

PSANCAM 57 E5E7 

PSANCAM 

 

PSANCAM 

BrdU E5E7 BrdU 

FITC 

PSANCAM 

BrdU 10 E6 

BrdU 

E5 BrdU E6 E7 

BrdU PSANCAM 

E5E7 PSANCAM 

 

 

P 

mRNA 

1 2 1 

1 

2 

 

 

GnRH 

mRNA 

mRNA 2.5 3.5 

3.5 

4 GnRH 

mRNA 

mRNA 

 

Tol2GFP 2.5 

1619 GFP 

GnRH GFP 

mRNA 

mRNA 

mRNA GnRH 

GnRH 

 

P 

CNTF 

 

 

 

 

 

cuprizone 

MBPCNPase 

 

 

 

 

cuprizone 

 

 

MBPCNPase 

 

CNTF 

 

CNTF 

P 

 

 

 

 

 

 

 

E1313.5 E11.5 

 

Islet1Lim3Lhx3 MNR2 

MMCl Lim3 

 

Islet1Lim3Foxp1 

E11.5 

Islet1 Lim3 

 

Foxp1 

 

P 

 

 

 

 

 

 

 

 

6

117 141 

P 

kirrel 

 

1 1 1 1 2 

1 

1 

2 

 

A member of the immunoglobulin superfamily, kirrel3, is expressed in the dorsal 

root ganglia DRGs. In the previous report, we have suggested that kirrel3 

may play a role in the development of tyrosine kinase receptor Trk Cpositive 

neurons. To get further insights into the function of kirrel3 in the DRG neurons, 

we characterized kirrel3expressing neurons in the DRGs of kirrel3lacZ Z knockin 

mice. In the lumber DRG neurons, the expression of βgalactosidase βgal 

was first observed at embryonic day 12.5, gradually increased, and reached 

the maximum level at postnatal day P 7. During embryonic stages, βgal was 

predominantly expressed in TrkA and TrkCpositive neurons, while some βgal 

positive neurons contained TrkB or Ret. The expression of Ret was increased in 

βgalpositive neurons between P7 and P14, and was observed in approximately 

43% of βgalpositive neurons of the adult DRGs. These results suggest that 

kirrel3 may be involved in the development of nociceptive and mechanoceptive 

neurons in addition to proprioceptive neurons. 

This work was supported by a GrantinAid for Scientific Research B from 

Japan Society for the Promotion of Science 22390036. 

P 

 

 

 

 

 

 

 

 

 

 

 

BMP4 FGF2 

 

Dlx5Six1Eya2 PCR Dlx5 

10 

GATA3/keratin19 Slug 

Sox2 FGF2 

FGF8 

BMP4 FGF2 

 

P 

/ 

Sprouty 

1,2 2 3 2 

1 

2 

3 

 

 

in vitro 

bFGF 

Receptor tyrosine kinase RTK 

Sprouty 4 Sprouty 


 

Sprouty4 Sprouty4 

 

Sprouty4 

 

Sprouty4 

Sprouty4 

 

 

P 

 

 

 

4 Oct3/4Sox2Klf4cMyc 

induced pluripotent stem cell 

iPS 

 

 

 

 

 

 

 

iPS 

 

P 

 

1 2 3 2 

1 

2 3 

P 

Dlg 

1 1 2 

1 

2 

 

 

 

 

 

 

3 16 Hox11Tcf21 

WT1Barx1Nkx3.2 

mesothelium 

 

 

Fgf16 FGF receptor12 

mesothelium 

Fgf16 FGF receptor12 

 

 

 

Dlg1 MAGUK 

Dlg1 Dlg1 -/- 

 

Dlg1 

Dlg1 -/- 

Dlg1 

2010 Wnt Fzd1 

2 Dlg1 -/- 

Fzd1 -/- Fzd2 -/- 

Dlg1 -/- 

 

Fzd1 -/- Fzd2 -/- 

Dlg1 -/- 

Notch 

Notch 

 

Dlg1 -/- 

Dlg1 Notch

142 

117 

P 

 

sox 

 

 

 

- 

 

 

200 sox 

sox9 sox9 

sox9 

 

in vitro siRNA sox9 

sox9 

 

 

sox9 

α 

 

sox9 - 

 

P 

Protocadherin a 

 

 

Pcdh 

Pcdh10a 

Pcdh10b Pcdh 

 

 

 

Pcdh10a 

Pcdh10a 

 

Pcdh10a 

 

P 

Developmental Origins of Health and Disease DOHaD 

 

Randeep Rakwal 

 

 

 

 

DOHaDDevelopmental Origins of Health and Disease 

 

 

50% 

1018 18 

mRNA 

DyeSwap DNA 

5500 4000 

200 

 

Trib1 

 

H1 H3 G Gpr88 

 

 

P 

α 

1 2 1 

1 

2 

 

 

β α 

 

α 

Ca CADM1 SynCAM 

α 

α 

SynCAM 

 

SynCAM KO α 

α 

 

P 

Occludin p 

 

1 2 

1 

2 1 

OccludinOCLN 

p63 

 

SMG 11E11 12 

 

 

OCLN p63 

E1213 ICR p63 

OCLN SMG 

p63 OCLN 48 

TB p63 

OCLN E13 p63 E14 

p63 TB OCLN 

TB TB OCLN 

p63 E18 

p63 OCLN 

TB OCLN 

 

P 

 

 

1 2 1 2 3 4,5 

1 

1 

2 3 

4 

5 

 

 

Ca 2+ 

 

L Ca 2+ 

Ca 2+ 

TEM 

12 nm 

 

 

Ca 2+ induced Ca 2+ release CICR 

 

CICR 

 

CICR

117 143 

P 

 

 

 

 

prechordal chondrocranium 

trabeculae 

trabecula communis 

 

 

 

intertrabeculae 

intertrabeculae 

 

PNA PNA 

 

PNA 

PNA 

PNA 

 

 

P 

 

 

 

 

 

 

1 2 

3 

 

13 DiI 

15 DiO 

 

22 1 2 

3 4 

 

24 1 2 

3 4 

 

 

 

 

 

 

P 

 

 

 

 

 

 

 

2 

2 

PET 

 

Aoyama et al., 2005PET 

24 7 

 

PET 24 

 

 

 

/ 

 

 

P 

LEFSP 

1 2 1 

1 

2 

LEF1 Wnt/ßcatenin 

 

SP6 Wnt/ßcatenin BMP 

LEF1 LEF1 

SP6 in vivo 

LEF1 SP6 

 

19 10 

LEF1 SP6 

19 SP6 

LEF1 

10 SP6 

 

LEF1 SP6 

LEF1 SP6 LEF1 

 

LEF1 

 

P 

 

1 2 3 1 

1 

1 2 

3 

 

Cynops 

pyrrhogaster 

 

 

 

/ TH/THR 

 

insitu 

THR 

 

 

THR 

2324 

B23792117 

P 

TTF 

1,2 1 1 1,2 2 1 

1 

2 

 

 

 

 

 

TTF1 

0 00 0 E0 

E10E12 ddy 6 10 μm 

1 TTF1 

LSAB DAB 

TUNEL HistoGreen 

 

E10.0 

E10.75 E11.0 

E11.25 E10.25 TTF1 

E11 

TTF1 

E11.25

144 

117 

P 

The role of Rho signaling pathway in dental epithelial stem cells 

Keishi Otsu 1 , Ryota Kishigami 1 , Ai OikawaSasaki 1 , Naoki Fujiwara 1 , 

Kiyoto Ishizeki 1 , Hidemitsu Harada 1 

1 

Dept. Anatomy, Iwate Med. Univ., 2 Advanced Oral Health Science Research 

Center, Iwate Med. Univ. 

We have recently found that Rho signaling plays an important role in ameloblast 

differentiation through maintenance of the cell polarity Otsu et al. Journal of 

Cellular Physiology, 2010. Consequently, we next focused on the role of Rho 

signaling in dental epithelial stem cells. In this study, we examined the function of 

ROCK, downstream Rho effectors, in dental epithelial cells isolated from mouse 

incisor apical bud. After treatment of ROCK inhibitor and knocking down ROCK 

by siRNA, the shape of cells changed from epithelial phenotype to mesenchymal 

like cells, and cellcell adhesions were lost. Real time RTPCR showed that 

ROCK inhibitor decreased expression of epithelial cell markers, and increased 

mesenchymal and stem cell markers. In the treated cells, mRNA expressions 

of slug, which is epithelialmesenchymal transition markers, increased and the 

intense nuclear accumulation was observed. In mouse incisor, slug was strongly 

expressed in apical bud compared to differentiated ameloblasts. Based on the 

data, we consider that Rhoslug signaling pathway is closely involved with the 

maintenance of dental epithelial stem cells. 

P 

Localization of osteopontin and osterix in periodontal tissue during 

orthodontic tooth movement in rats 

Ji Youn Kim, Byung In Kim, Seong Suk Jue, Jae Hyun Park, Je Won Shin 

Department of Oral Anatomy, Graduate School of Dentistry, Kyung Hee 

University, Seoul, KOREA 

The aim of this study was to evaluate the localization of osteopontin OPN 

and osterix in periodontal tissue during experimental tooth movement with 

heavy force in rats. Nickeltitanium closedcoil springs were used to create a 

100g mesial force to the maxillary first molars. On days 3, 7, 10, and 14 after 

force application, histological changes in periodontal tissue were examined by 

immunohistochemistry using proliferating cell nuclear antigen PCNA, OPN, and 

osterix. PCNApositive cells were found close to the alveolar bone and cementum 

on both sides. OPNpositive cells were observed along the cementing line of the 

cementum and bone on both sides and also were visible along with newly formed 

fibers in the periodontal ligament on the tension side. Osterixpositive cells were 

strongly detected on the surface of the alveolar bone and cementum on both sides. 

During tooth movement, periodontal remodeling occurs on both sides. These 

results indicate that OPN and osterix may play an important role of differentiation 

and osteoblasts and cementoblasts matrix formation during periodontal tissue 

remodeling. 

P 

Fetal jaw movement affects molecular cascade in the development of 

mandibular condylar cartilage 

Esrat Jahan 1,2 3 1 Ashiq Mahmood Rafiq 1,2 

2 1 

1 

2 

3 

Fetal jaw movement restriction has been shown to cause deformity of the 

mandibular condyle. We hypothesized that this treatment affects the expression 

of mechanosensitive molecules, namely Indian hedgehog Ihh and bone 

morphogenetic protein2 Bmp2 in the condyle. We restrained jaw movement 

by suturing the jaw of E15.5 mouse embryos and allowed them to develop 

until E18.5 using exo utero system. Morphological, histomorphometric and 

immunohistochemical study showed that the mandibular condylar cartilage 

was deformed, volume and total cell number were reduced, and number and/ 

or distribution of 5bromo2′deoxyuridinepositive cells, Ihh and Bmp2 

positive cells in the mesenchymal and prehypertrophic zones were significantly 

and correspondingly decreased in the sutured group. Our results revealed that 

the mechanical stress induced by prenatal jaw movement restriction decreased 

proliferating cells, the amount of cartilage, and altered expression of the Ihh and 

Bmp2, suggesting that Ihh and Bmp2 act as mechanotransduction mediators in 

the development of mandibular condylar cartilage. 

P 

 

1 2 

1 

2 

 

 

 

 

 

 

 

 

 

 

FGF18 

 

 

 

 

Phase Field 

 

 

P 

 

 

1 1 1 2 3 1 

1 

2 3 I 

 

Muscle 

tendon junction: MTJMTJ 

ArgGlyAsp 

RGD 

RGDserine S 

 

MTJ 

RGDS 

ECM 

RGDS 

 

RGDS 

RGDS 

 

RGDS 

MTJ 

P 

 

1 2 2 3 4 

4 5 1 1 

1 

2 

3 

RI 4 5 

 

 

 

 

19 4 

 

 

 

 

 

ECM 

iTRAQQ 

NanoLC 

MALDITOF/TOF MS/MS 

 

core protein collagen GAG 

Type I & II collagen GAGType I collagen 

Type II collagen 

 

 

ECM

117 145 

P 

FIB/SEM 

1,3 1 2 2 3 

1 

1 

2 

3 

 

 

 

 

FIB/SEM 

CT 

8 

5%EDTA 

5 1 OsO 4 

EPON812 H7650 

FIB/SEM 

en block EPON812 

, FIB/SEM 

Quanta 3D FEGFEIFIB 

Ga 1 nA 80 nm/step 

80 μm 

 

P 

Molecular sequence and distribution of the NMDA receptor subunit 

NR mRNA in the central nervous system of pigeons Columba livia 

Mohammad Rabiul Karim 1,2 , Shouichiro Saito 1 , Yasuro Atoji 1 

1 

Gifu Univ, Fac Appl Biol Sci, 2 Bangladesh Agri Univ, Fac Vet Sci 

NR1 is a key subunit of the NmethylDaspartate type of glutamate receptors 

maintaining the glutamatergic system in the mammalian central nervous system 

CNS. In the present study, cDNA sequence and expression pattern of NR1 

mRNA in the pigeon CNS were examined. From cDNA sequence analysis, its 

predicted amino acid sequence was revealed to show 96%, 95% and 85% identity 

to that of the chicken, zebra finch and human NR1, respectively. By RTPCR, 

pigeon NR1 mRNA was revealed to be highlevel in the olfactory bulb, pallium 

and subpallium of the telencephalon and in the cerebellum, mediumlevel in the 

diencephalon and optic tectum, and lowlevel in the lower brainstem and spinal 

cord. By in situ hybridization, intense hybridization signal was observed in the 

olfactory bulb, pallium except entopallium, septal nuclei and striatum of the 

telencephalon, and the granular and Purkinje cell layers of the cerebellum, and 

weak signal in the thalamus, optic tectum, lower brain stem and gray matter of 

the spinal cord. This study revealed the similarity of NR1 expression between the 

pigeon and mammals, indicating the functional importance of the glutamatergic 

system in the avian CNS. 

P 

 

 

 

 

 

 

2 VGLUT2mRNA 

VGLUT2 

 

 

0.1% 4 

50 μm VGLUT2 C 

 

 

 

HVC, RA, area X 

 

MC, 

An, NL , MC 

VGLUT2mRNA 

 

 

P 

SULTBa 

 

 

 

 

 

 

GABA A 

GABA 

 

SULT2A1 SULT2B1a 

mRNA 

 

 

SULT2B1a SULT2B1a 

SULT2B1a 

 

SULT2B1a 

 

P 

 

 

 

DGDGK 

C DG 

DGK DGK 

DGKε DG 

DGKε 

DGKε 

DGKε 

64 kDa 

 

DGKε 

DGKε 1 

IP3R1 

DGKε 

IP3R1 

P 

Androgen receptor expression in the preoptic and anterior 

hypothalamic areas of the adult male rats and mice 

Jahan Mir Rubayet, Keiji Kokubu, Chikahisa Matsuo, Islam Md. Nabiul, 

Akie Yanai, Ryutaro Fujinaga, Koh Shinoda 

Functional Neuroanatomy, Yamaguchi Univ. Grad. Sch. of Med. 

Species difference has been suggested on androgeninduced functions of the 

preoptic and anterior hypothalamic areas PO/AH. Using paraformaldehyde 

fixed completely serial frozen sections, expression of androgen receptor AR was 

immunohistochemically compared in the PO/AH between adult male rats Wistar, 

SD and mice C57BL/6, DBA/2j, Balb/c. In general, AR expression was stronger 

in mice than rats, particularly in the medial preoptic area MPO, posterodorsal 

preoptic nucleus, dorsal PO/AH junction DPAJ and suprachiasmatic nucleus 

SCN. Exceptionally, more ARimmunoreactive ARir cells were seen in the 

sexually dimorphic nucleus SDN of the MPO and periventricular zone of the AH 

in rats. In addition, we found a distinct mousespecific ARir neuronal cluster as 

the “tear drop nucleus (TDN)” dorsal to the SCN, which resembles the rat SDN or 

mouse putative SDN in calbindin immunoreactivity, and two distinct ratspecific 

small clusters as the “DPAJ island” and “paraventricular island”. The present data 

might explain distinct androgeninduced psychotic, behavioral and endocrinergic 

responses between the two rodents, warning that data cannot directly be applied 

each other.

146 

117 

P 

GABA 

parvalbumin 

1 1 1 1 1 

1 2 3 1 

1 

2 

3 

MG GABA 

parvalbuminPV 

 

ABC GABA 

PV ir GABAir MG 

MGd MGv MGm 

GABAir 

MGm GABAir MGv 

MGd MGv PVir calbindinir 

MGv MGd PVir 

MGv MGm MGm MGv PVir 

PVir PVir 

MGv MGm GABAir PVir 

MGd PVir 

GABAir 

 

 

 

P 

SNARE 

 

 

SNARE 

 

SNARE 

 

 

 

SNARE 

 

SNARE syntaxin1, SNAP25, VAMP2 

 

 

 

SNARE 

 

 

SNARE 

SNARE 

 

P 

GABA 

1 2 3 4 2 1 

1 

2 

3 4 

, 

TGNC 

SC GABA GABA 

GABA NC 

GABA NC GABA 

DNDS GABA GAT 

SC DNDS 

NC 

GABA 

GABA free 24 TG 

NC GABA SC GABA 

100 μM GABA 24 NC 

SC GABA GABA GAD 

GABA GAT NC SC 

 

NC GAT GABA SC 

GABA 

GABA 

NC SC 

 

P 

Expression of AMPA type glutamate receptor in developing chick 

vestibulocochlear ganglia 

 

1 

Expression of AMPA type glutamate receptor GluR14 in the vestibular VG 

and cochlear ganglia CG in developing chick embryo was examined. VG and 

CG at the embryonic day 10 E10, E12, E14, E16, E18 and E20 were prepared 

for reverse transcriptasePCR RTPCR and immunohistochemistry IHC. In 

the RTPCR study, GluR1 mRNA was weakly expressed from E10 to E20 in both 

ganglia. Expression of GluR2 mRNA was intensive at E10 and E12 in VG and at 

E10E14 in CG, and then, it was gradually weakened from E14 in VG and from 

E16 in CG. Expression of GluR3 mRNA in both ganglia was weak at E12E18 

and it was extremely augmented at E20. GluR4 mRNA was moderately expressed 

at E10E14 in VG and at E10E16 in CG, and then, it was gradually increased 

from E16 in VG and from E18 in CG. In the IHC study, immunoreactivity of 

GluR2 was observed in the cytoplasm and process and that of GluR2/3 and 

GluR4 was in the cytoplasm of the VG and CG cells, while that of GluR1 was 

never detected. These results suggest that GluR2, GluR3 and GluR4 are the major 

subunits of AMPA receptor during the development of the chick embryo VG and 

CG cells. They would have different roles for the development. 

P 

 

 

1 Mohammad Rabiul Karim 1,2 1 

1 

2 Faculty of Veterinary Science, 

Bangladesh Agricultural University 

 

 

3 

VGLUT 1 

 

 

1 

VGLUT 

 

 

RTPCR in situ hybridization RT 

PCR 2 3 VGLUT 3 VGLUT 

2 VGLUT mRNA 


 

 

2 VGLUT 

P 

C 

 

 

N 2 

C PapillonLefevre 

HaimMunk 

C 

 

D 

 

8 

 

CA2fasciola cinereumindusium griseumtenia tecta 

 

 

 

 

25 

8

117 147 

P 

 

 

 

VIP 

12 

 

VIP A, B 

2receptor 

12 

 

 

 

 

VIP 

VIP VIP 

 

A,B 2receptor 

A, B 

2receptor 

 

 

P 

 

 

 

 

PGP9.5 

 

 

1 

3.25 TH CGRP 

CGRP 

 

 

 

1 μm 

 

 

 

2, 3 

 

 

 

P 

 

1 2 3 1 

1 

2 3 

 

 

 

 

 

 

 

Protein gene product 9.5 PGP9.5 

56, 67, 72, 88 4 6 10 

10 

 

415 

30/PBS 24 20 μ 

6 

 

 

 

 

 

P 

 

 

 

 

 

 

 

 

 

 

 

N- 

N 

N 

 

 

N 

 

N 

 

 

P 

CART 

 

 

CARTCocaine and AmphetamineRegulated Transcript 

 

 

 

CART 

Sauropsida 

CART 

 

 

CART 

 

CART 

CART 

 

CART 

 

-CART 

CART 

P 

GABA 

 

 

 

 

 

GABA 

 

GABA 

 

 

 

GABA 

 

GABAA 

α1 

G 

2 

GABA

148 

117 

P 

Conserved properties of dendritic trees in four cortical interneuron 

subtypes 

1,2 1,2 2,3 1,2 

1 

2 CREST 3 

 

Dendritic trees influence synaptic integration and neuronal excitability, yet 

appear to develop in rather arbitrary patterns. Using electron microscopy and 

serial reconstructions, we analyzed the dendritic trees of four morphologically 

distinct neocortical interneuron subtypes to reveal two underlying organizational 

principles common to all. First, crosssectional areas at any given point within 

a dendrite were proportional to the summed length of all dendritic segments 

distal to that point. Consistent with this observation, total crosssectional area 

was almost perfectly conserved at bifurcation points. Second, dendritic cross 

sections became progressively more elliptical atmore proximal, larger diameter, 

dendritic locations. Finally, computer simulations revealed that these conserved 

morphological features limit distance dependent filtering of somatic EPSPs and 

facilitate distribution of somatic depolarization into all dendritic compartments. 

Because these features were shared by all interneurons studied, they may represent 

common organizationalprinciples underlying the otherwise diverse morphology of 

dendritic trees. 

P 

FABP in astrocytes is involved in control of neuronal dendritic 

formation 

Majid Ebrahimi, Yshiteru Kagawa, Kazem Sharifi, Yasuhiro Adachi, 

Tomoo Sawada, Nobuko Tokuda, Yuji Owada 

Department of Organ Anatomy, Yamaguchi University Graduate School of 

Medicine 

Alteration of fatty acid homeostasis in brain is associated with human functional 

psychosis. We previously showed that FABP7 braintype fatty acid binding 

protein, a cellular fatty acid chaperon abundantly expressed in astrocytes, is 

involved in control of emotional behavior. However, mechanism by which FABP7 

in astrocytes modulates neuronal activity is still unknown. 

Mixed cortical culture and neuron/astrocyte coculture were established from 

wildtype WT and FABP7 KO KO mice, and morphology of pyramidal 

neurons was evaluated by NeuronMetrics. GolgiCox staining was performed to 

examine dendrite morphology of neurons in cerebral cortex of KO mice. 

In mixed cortical culture, pyramidal neurons of KO mice showed decrease in 

length and number of dendritic branches. In the WT neuron/KO astrocyte hybrid 

coculture WTn/KOa such changes in dendrite morphology were also detected 

compared to WTn/WTa coculture. Moreover, pyramidal neurons in prefrontal 

cortex of KO mice showed aberrant dendrite formation. 

FABP7 may regulate neuronal dendrite formation by modulation of lipid 

homeostasis metabolism/signal transduction in astrocytes. 

P 

FILIP 

1 1 1 1 1 1 

1,2 1 

1 

 

2 

 

 

 

 

 

 

 

FILIP 

A 

 

FILIP 

FILIP 

 

 

P 

CA 

 

 

 

 

 

 

CA1 

 

Thy1 GFP 

9 2 

mushroom type stubby type thin type 

CA1 Western blot 

 

BDNF PSD95 

BDNF PSD95 

 

 

 

BDNFPSD95 

 

P 

In vivo analysis of postsynaptic molecular dynamics in the 

developing mouse cortex 

 

 

Dynamic behavior of postsynaptic molecules is thought to play important 

roles in synaptic transmission and plasticity. Postsynaptic densities PSDs and 

spines are key elements in the process of postsynaptic signal transduction. We 

previously reported differential dynamics of molecules present in PSDs and 

spines in cultured hippocampal neurons. Photobleaching of actin, the prominent 

cytoskeletal component in the spine cytoplasm, and PSD scaffolding proteins 

PSD95 and Homer1c showed distinct mobility in the postsynaptic cytoplasm, 

indicating independent regulation of molecular dynamics. To gain more insights in 

the protein mobility at the postsynaptic sites and its regulation, information from 

neurons in the native environment should be obtained. To monitor protein mobility 

in vivo, we applied twophoton photoactivation/bleaching to pyramidal neurons 

in the mouse cortex and monitored fluorescence decay/recovery in individual 

spines at different developmental stages. Lifetime of PSD/spine structure was 

much longer than resident times of individual protein molecules, indicating the 

importance of protein network in maintenance of the postsynaptic structural 

organization. 

P 

Direct monitoring of AMPA receptor recycling and trafficking 

Ayako Hayashi 1 , Daisuke Asanuma 2 , Mako Kamiya 3 , Yasuteru Urano 3 , Shigeo 

Okabe 1 

1 

Department of Cellular Neurobiology, Graduate School of Medicine, The 

university of Tokyo, 2 Department of Neurobiology, Graduate School of Medicine, 

The university of Tokyo, 3 Department of Chemical Biology and Molecular 

Imaging, Graduate School of Medicine, The university of Tokyo 

Longlasting change of synaptic efficacy, such as LTP, is known to be correlated 

with increase of postsynaptic response of AMPAtype glutamate receptors 

AMPARs. AMPAR targeting is regulated by surface mobility and exo/ 

endocytosis. Although single particle tracking revealed contribution of AMPAR 

lateral mobility in their trapping at the synaptic sites, dynamic recycling of 

AMPARcontaining vesicles after plasticityinducing stimuli has not yet been 

clarified. We expressed AMPAR subunit GluR1 tagged with ZIPbinding cassette 

in primary hippocampal neurons and visualized cell surface AMPARs by labeling 

with ZIP peptides conjugated with pH sensitive dye RhPM. RhPMlabeled 

GluR1 increased its fluorescence after endocytosis, due to the acidification in 

the endosomal compartment. To visualize the intracellular transport of GluR1 

containing vesicles after endocytosis, we monitored both superecliptic pHluorin 

tagged GluR1 and RhPMlabeled GluR1 simultaneously. We identified that GluR1 

endocytosis occurred on dendritic shafts and also in larger spines. Reciprocal 

signal change of SEP/RhPMlabeled receptors facilitates the identification of exo/ 

endocytotic events within dendritic spines.

117 149 

P 

Motor protein KIFA is essential for hippocampal synaptogenesis 

and learning enhancement in an enriched environment 

 

 

Environmental enrichment causes a variety of effects on brain structure and 

function. Brainderived neurotrophic factor BDNF plays an important role 

in enrichmentinduced neuronal changes; however, the precise mechanism 

underlying these effects remains uncertain. In this study, a specific upregulation of 

kinesin superfamily motor protein 1A KIF1A was observed in the hippocampi 

of mice kept in an enriched environment and, in hippocampal neurons in vitro, 

BDNF increased the levels of KIF1A and of KIF1Amediated cargo transport. 

Analysis of Bdnf +/- 

and Kif1a +/- mice revealed that a lack of KIF1A upregulation 

resulted in a loss of enrichmentinduced hippocampal synaptogenesis and learning 

enhancement. Meanwhile, KIF1A overexpression promoted synaptogenesis via 

the formation of presynaptic boutons. These findings demonstrate that KIF1A 

is indispensable for BDNFmediated hippocampal synaptogenesis and learning 

enhancement induced by enrichment. This is a new molecular motormediated 

presynaptic mechanism underlying experiencedependent neuroplasticity. 

P 

Activationspecific Changes of Angiotensin II Receptors in Mouse 

Cerebellum and Adrenal Glands with In Vivo Cryotechnique 

Zheng Huang, Nobuo Terada, Yurika Saitoh, Jiaorong Chen, Shinichi Ohno 

Department of Anatomy and Molecular Histology, Interdisciplinary Graduate 

School of Medicine and Engineering, University of Yamanashi 

We have performed immunohistochemical analyses of AT1/2R with molecular 

activationspecific antibodies in living mouse cerebellum and adrenal glands under 

normal or hypoxic conditions with “in vivo cryotechnique” IVCT followed by 

freezesubstitution FS. In the cerebellum, outer molecular layers and some areas 

around Purkinje cells were immunostained as dotted patterns, which were closely 

related to Bergmann glia. At 5 and 10 min after hypoxia, the immunoreactivity 

was remarkably reduced, though it was still remained the same in the adrenal 

glands at 15min after hypoxia. This immunohistochemical approach with IVCT 

FS enabled us to perform their precise analyses. 

P 

Nodose ganglion cells expressing melanocortin receptor send their 

fibers to the pancreatic islets in the mouse 

Toshiko Tsumori, Tatsuro Oka, Jianguo Niu, Yukihiko Yasui 

Dept. Anat. & Morphol. Neurosci., Shimane Univ. Sch. Med. 

Melanocortin system including melanocortin4 receptor MC4R plays a 

crucial role in the control of feeding and energy expenditure. Using a transgenic 

mouse model in which green fluorescent protein GFP is produced under the 

control of the MC4R promoter, we recently reported that the dorsal motor 

nucleus of the vagus nerve contains many MC4Rexpressing neurons which 

project to the intrapancreatic ganglia. In this study, we examined whether or 

not MC4Rexpressing nodose ganglion cells send their fibers to the pancreas. 

Using retrograde tracing with chorela toxin B subunit in combination with 

immunohistochemistry for GFP, we demonstrated that some of the nodose 

ganglion cells sending their fibers to the pancreas showed GFP immunoreactivity. 

Using double immunofluorecence staining for insulin and GFP, we also showed 

that GFPimmunoreactive ir boutonlike varicosities were distributed within 

the pancreatic islets and some of them were in close apposition to insulinir cell 

bodies. The previous and present results suggest that both of the vagal afferent 

and efferent neurons expressing MC4R may be closely related to exocrine and 

endocrine pancreatic functions on energy expenditure. 

P 

Runx 

 

 

Runx runt Runx13 3 

Runx1 Runx1 

12.5 E12.5 

Runx1 

Runx1 Runx1 -/- ::TgE12.5 

Runx1 E17.5 

Runx1 Runx1 

Runx1 

 

Runx1 

NeurofilamentM 

VAChTRunx1 +/+ ::Tg Runx1 -/- ::Tg 

 

3 

Runx1 -/- ::Tg VAChT 

Runx1 +/+ ::Tg 

Runx1 -/- ::Tg Runx1 

 

P 

A 

 

1 2 1 

1 

2 

A Cholecystokinin A receptor: CCKAR 

CCK CCKAR mRNA 

nodose ganglion: NGNG CCK 

binding site 

CCKAR CCKAR 

 

CCK 

AR CCKAR 

2 

VGluT2 

CCKAR 

CCKAR NG 

 

B 

NG CCKAR/ 

VGluT2 CCKAR 

CCKAR 

NG 

 

P 

Features of boutons distribution along axons of neurons in the 

caudal nucleus of tractus solitarius of the rat 

 

 

A large amount of the information processing that happens in the brain occurs 

in the microcircuitry. For comprehensive understanding of the mechanisms 

regulating connectivity among neurons within the microcircuitry, it is essential 

to elucidate how pre and postsynaptic components are connected. In this study, 

to understand the rules by which axons make synapses, we investigated the 

arrangement of synaptic boutons on axons of biocytinfilled neurons in the caudal 

nucleus of the tractus solitarius cNTS of medulla oblongata. We analyzed the 

length and distribution of interbouton intervals ibi under the light microscope. 

The overall average length of ibi was 4.7 μm and varied by neurons ranged 

from 2.8 to 8.1 μm. Although there was considerable variation in the average 

length of ibi among neurons, a general scaling relationship between the standard 

deviation and the average was found. In addition, the distribution of ibi was 

characteristically skewed and the tail of that was fitted well to an exponential 

distribution. These results suggest that the synaptic boutons along axons of cNTS 

neurons are distributed in a fundamentally similar manner, a random manner.

150 

117 

P 

Calbindin 

 

 

 

mitral 

cellMCMC 

MC mitral cell layer 

MCL olfactory cortex 

granule cell GC 

GC MC reciprocal synapses 

 

 

MCL CB 

Neurolucida 

 

CB MCL 

2-4 

GC 

 

 

 

P 

GALP 

1 2 2 1,3 1 

1 

2 3 

 

GALP 

 

GALP 

mRNA 

GALP 

CreloxP GFP 

GFP β 

Tg Tg 

7 

GFP 

β 

GALP 

 

GALP 

 

 

P 

Origins of nitric oxide pathways to the median preoptic nucleus 

Hitoshi Kawano, Sadahiko Masuko 

Saga University 

It has been revealed that many nerve fibers containing nitric oxide NO as a 

neurotransmitter are found in the median preoptic nucleus POMe by means of 

NADPHdiaphorase histochemistry. We investigated where the NO pathways 

terminating at the POMe come from, using retrograde tracing method and 

immunohistochemistry for NO synthase NOS, a marker for NO neurons. After 

injection of a retrograde tracer in the POMe, a larger number of retrogradely 

labeled neurons in the subfornical organ SFO showed NOS immunoreactivity. In 

addition, a few neurons were doublelabeled in the parvocellular paraventricular, 

the periventricular, and the ventromedial nuclei of the hypothalamus. The finding 

indicates that the principal NO pathway to the POMe comes from SFO. 

P 

Kisspeptin Tuberoinfundibular dopamine 

TIDA 

1 2 1 1 1 

1 

2 

kisspeptin HPG axis 

kisspeptin 



 

kisspeptin 

kisspeptin 

 

Tuberoinfundibular dopamine neuronsTIDA 

neuronskisspeptin TIDA neuron 

kisspeptin 

TH synaptophysin 

kisspeptin 

TIDA neuron 

kisspeptin TIDA neuron 

in vitro kisspeptin 

kisspeptin 

TIDA neuron 

 

P 

 

1 2 

1 

2 

P 

CA 

1 2 

1 

2 

 

 

 

AD30 29 

 

116 B 

CTb 

AD 

2.5 kg 

CTb 7 CTb 

AD 

30 CTb AD 

30 AD 

29 

AD 

AD AD 30 

29 

 

 

 

 

 

 

 

 

1 

 

CTb 

CA1 

CA1 BDA 

VVI 

CA1 

 

CA1

117 151 

P 

 

 

 

 

 

 

 

 

 

 

 

 

parvicellular 

part of the ventral posterior nucleus of the thalamus VPPC 

retrouniens area central medial thalamic nucleusparafascicular 

thalamic nucleusparvicellular part of the subparafascicular thalamic nucleus 

oval paracentral thalamic nucleus paraventricular thalamic nuclei 

rhomboid thalamic nucleus 

VPPC 

 

 

P 

Classification of rat pallidofugal projection systems revealed by 

singleneuron tracing study with a viral vector 

1,2 1 1 1 1 

1 

2 JST, CREST 

The main striatofugal system is divided into two neural populations, the 

striatonigral neurons striatal direct pathway neurons and the striatopallidal 

neurons striatal indirect pathway neurons. However, the striatal direct pathway 

neurons also give collaterals in the globus pallidus GPe, which is a relay nucleus 

in the indirect pathway of the basal ganglia Kawaguchi et al., 1990; Fujiyama 

et al., 2011. We also found the targeted region in GPe were different between 

the striatal direct and indirect pathway neurons Fujiyama et al., 2011. Our next 

question is whether the GPe neurons located in the different regions targeted 

by striatal direct and indirect pathway neurons show the same axonal trajectory 

or not. To reveal it, the single GPe neurons in rat were labeled by recombinant 

Sindbis virus that is designed to express the membranetargeted green fluorescent 

protein. In the present study, we found two types of axonal trajectory of GPe 

neurons in the different regions and discuss the possibility that the GPe can be 

separated functionally to be involved in the different network of the basal ganglia. 

P 

A Morphological Analysis of Thalamocortical Axon Fibers of Rat 

Posterior Nuclei: A Single Neuron Tracing Study with Viral Vectors 

1,2 1 1 1 1 

1,4 3 3 2 1 

1 

2 

3 4 JST, CREST 

The posterior thalamic nuclei POm receives inputs from the spinal cord and 

trigeminal nuclei, and projects to the primary somatosensory S1 cortex and other 

cortical areas. Although thalamocortical axons of single ventral posterior nuclei 

neurons are well known to innervate layer L 4 of the S1 cortex with distinct 

columnar organization, those of POm neurons have not been elucidated yet. In 

the present study, we investigated complete axonal and dendritic arborizations 

of single POm neurons in rats using Sindbis viral vectors. When we divided the 

POm into anterior and posterior parts according to calbindin immunoreactivity, 

dendrites of posterior POm neurons were wider but less numerous than those 

of anterior neurons. More interestingly, axon fibers of anterior POm neurons 

were preferentially distributed in L5 of the S1 cortex, whereas those of posterior 

neurons were principally spread in L1 with wider and sparser arborization than 

those of anterior neurons. These results suggest that the POm is functionally 

segregated into anterior and posterior parts, and that the two parts may play 

different roles in somatosensory information processing. 

P 

Morphological analysis of axon collaterals derived from single 

corticospinal neurons in subcortical structures 

 

 

The corticospinal tract which conveys the final command for control of voluntary 

movement has been known to give axon collaterals to various cortical and 

subcortical regions. The target regions thus receive the same information with that 

gives rise to muscle actions. To assess the collaterals in the subcortical regions by 

a morphological approach, we visualized axons of single corticospinal neurons 

by injecting a recombinant sindbis virus expressing membranetargeted GFP into 

the pyramidal tract. The labeled cortical neurons were distributed in layer V of 

the motor cortex and motorsensory corticesareas FL and HL. The corticospinal 

neurons gave multiple axon collaterals to various subcortical structures and 

especially sent branches, with high frequency, to the striatum 71%, zona incerta 

67%, pontine and reticulotegmental nuclei 100%, which are known to play a 

role in control of voluntary movement or gating of peripheral inputs to thalamic 

nuclei. These abundant subcortical collaterals suggest that the "copy" of final 

motor command from the cortex plays an important role in motor and sensory 

processing of the subcortical structures. 

P 

 

 

1 2 2 2 

1 

2 

 

MCH 

 

2VGLUT2 

MCH 

MCH VGLUT2 

 

SD MCH 

VGLUT2 mRNA in situ hybridizationISH 

MCH 

VGLUT2 cRNA 

 

MCH 

VGLUT2 

MCH 

MCH VGLUT2 

VGLUT2 

 

P 

Projections from the amygdaloid anterior basomedial and anterior 

cortical nuclei to MCHcontaining hypothalamic neurons of the rat 

 

Dept. Anat. & Morphol. Neurosci., Shimane Univ. Sch. Med., Izumo, Japan 

Melaninconcentrating hormone MCH is involve in the regulation of feeding 

behavior, and MCHcontaining neurons are distributed mainly in the lateral 

hypothalamus LH. The anterior bosomedial nucleus BMA and anterior cortical 

nucleus ACo of the amygdala have been known to project to the LH, and to 

form part of a circuit involved in processing olfactory, gustatory and visceral 

information related to feeding. However, it is still unknown whether or not MCH 

containing LH neurons are under the direct influence of the BMA and ACo. Here 

the organization of projections from the BMA and ACo to MCHcontaining LH 

neurons was examined and new data were provided as follows: 1 the prominent 

overlap of the distribution field of the BMA or ACo fibers and that of the MCH 

ir neurons is found in the ventrolateral LH; 2 the BMA and ACo axon terminals 

make asymmetrical synapses with MCHir neurons; and 3 most of the BMA and 

ACo neurons projecting to the ventrolateral LH are positive for VGLUT2 mRNA. 

These data suggest that the BMA and ACo of the amygdala may exert excitatory 

influence upon the MCHcontaining LH neurons for the regulation of feeding 

behavior.

152 

117 

P 

Inhibitory inputs of CCKpositive neurons to PVexpressing neurons 

in mouse neocortex 

1 1 1 1 2 

1 1,3 1 

1 

2 

3 CREST 

Neocortical GABAergic interneurons are roughly classified into three subgroups 

by chemical markers, parvalbumin PV, somatostatin SS and others such 

as vasoactive intestinal polypeptide VIP and cholecystokinin CCK. PV 

expressing neurons, fastspiking neurons, are a major component of GABAergic 

interneurons in neocortex. We generated transgenic mice expressing dendritic 

membranetargeted GFP selectively in PVexpressing neurons, and succeeded in 

visualizing somata and dendrites in a Golgi stainlike fashion. 

We previously investigated inhibitory inputs to PVexpressing neurons from PV, 

SS or VIPexpressing neurons, by combining immunofluorescence labeling of 

presynaptic and postsynaptic sites with antibodies to presynaptic markers and 

gephyrin, respectively. PV or SSpositive axon terminals made close contacts 

to the dendrite, whereas axon terminals of VIPexpressing neurons preferred the 

soma. 

In the present study, we visualize axon terminals of CCKexpressing neurons by 

immunofluorescence staining in the transgenic mice, observe the close appositions 

to PVexpressing neurons under confocal laserscanning microscope, and analyze 

the inputs quantitatively. 

P 

Elimination of somatic climbing fiber synapses proceeds with the 

differentiation of cerebellar interneurons 

 

 

In the developing cerebellum, the soma of individual Purkinje cells PCs is 

innervated by multiple climbing fibers CFs. Through their competition, only 

a single winner CF translocates to dendrites and the remaining somatic CF 

synapses are eliminated, leading to the establishment of CF monoinnervation. 

Concomitantly, basket cell fibers BFs begin to form inhibitory synapses on 

PC somata. Here, we examined anatomical relationship between declining CFs 

and developing inhibitory neurons at PC soma by light and electron microscopic 

analysis. In the first postnatal week of murine life, CFs innervated the entire 

somatic surface. Thereafter, BFs expanded their innervation from the apical 

side of PC somata downwards in synchrony with the reduction of somatic CF 

synapses. Furthermore, CF terminals frequently detached from the basal side of 

PC somata and often formed synapses on the somatodendritic domain of Lugaro 

cells LCs, a cerebellar interneuron lying just below the PC layer. These findings 

suggest that elimination of somatic CFs proceeds with the differentiation of 

cerebellar interneurons, such as BFs expelling somatic CF synapses and LCs 

accommodating perisomatic CF terminals. 

P 

Tectothalamic inhibitory neurons in the inferior colliculus receive 

converged axosomatic excitatory inputs from multiple sources 

 

 

Tectothalamic inhibitory TTI cells in the inferior colliculus IC are encircled 

by dense excitatory terminals positive for vesicular glutamate transporter 2 

VGLUT2. Four auditory brainstem nuclei including IC itself were identified 

as possible sources by examining mRNA expression of VGLUT1 and VGLUT2 

in ICprojecting cells. In this study, Sindbis/palGFP virus was injected in these 

nuclei to elucidate whether neurons in the nuclei make axosomatic contacts on 

TTI cells or not. Labeled neurons in all four nuclei made axosomatic contacts 

on large GABAergic neurons with dense axosomatic terminals, presumable TTI 

PTTI cells. Furthermore, a single axon made one to six contacts on a PTTI cell. 

In 3 cases, single IC excitatory cell was successfully labeled, and analyzed for 

spatial relationship between the labeled axon and PTTI cells. Single IC excitatory 

cell made axosomatic contacts on 1030 PTTI cells in the ipsilateral IC. Finally, 

double injection of Sindbis/palGFP and Sindbis/palmRFP viruses in 2 nuclei 

revealed convergence of inputs from 2 nuclei on a single PTTI cell. The results 

imply both divergence and convergence of auditory information on the cell bodies 

of TTI cells. 

P 

Axon terminals of the corticocollicular projection in the rat auditory 

system 

 

 

 

The corticocollicular projection was visualized by Sidbis virus and studied 

morphologically. The axons visualized with Golgi method in the inferior colliculus 

IC have been classified into three types and studied electronmicroscopically, A. 

J. Rocket and E. G. Jones, 1972,1973 with their origins mentioned prooflessly. 

We successfully visualized corticocollicular projection exclusively by infection 

of a recombinant Sindbis virus into cortical projection neurons in the auditory 

area. The Sindbis virus which expresses palmitoilationsiteadded GFP as the 

reporter gene is a kind gift from Dr. Kaneko in Kyotouniversity. The cortico 

collicular axon terminal images in the inferior colliculus was morphologically 

studied and compared with intrinsic axons from IC neuron which are stained in the 

same way. We further observed cortical projections into the nucleus of brachium 

of IC, external nucleus of IC, central nucleus of IC and deep layer of the superior 

colliculus. It should be noted that different types of termination exist in the above 

mentioned auditory brainstem. 

P 

Afferent projection to amygdaloid subnuclei and intrinsic connection 

of each subnuclei 

 

 

The amygdala is structurally diverse and comprised of several subnuclei. Extra 

and intraamygdala inputs into these subnuclei were extensively investigated by 

injection of CTb into various amygdaloid subnucleri, respectively. Ce received 

moderate to heavy inputs from almost all amygdaloid subnuclei, and from limbic 

cortex and hippocampus APir and AI, thalamus PV and MGM, hypothalamus 

VMH and midbrain PB. Me received moderate to heavy inputs from BM, Co, 

and from limbic cortex and hippocampus Pir, AHi and CA1, thalamus PV and 

hypothalamus VMH. BM received moderate inputs from Me, Co and BL, and 

from limbic cortex AI and thalamus PV. BL received moderate inputs from 

Me, La, BM, Co, and from limbic cortex and hippocampus LEnt, APir and CA1, 

thalamus PV and midbrain PB. La received moderate to heavy inputs from 

Me, Co, BM and BL, and from limbic cortex Ect and PRh, thalamus MGM 

and hypothalamus VMH. Co received light to heavy inputs from La, Me, and 

from limbic cortex and hippocampus AI, Pir and DEn and thalamus PV, PT and 

MGM. These subnucleispecific afferent projections are involved in emotional 

process in a different manner. 

P 

 

 

 

 

 

 

 

 

 

HeLa 

 

αtubulin Hec1CENPA

117 153 

P 

 

 

 

 

SEM 

 

 

75 mM KCl 

 

 

SEM 

 

SEM 

 

 

 

 

SEM 

 

P 

Communication between Flagellar Outer and Inner Dynein Arms 

Toshiyuki Oda, Toshiki Yagi, Masahide Kikkawa 

Dept. Cell Biology, Univ. Tokyo, Grad. Sch. Medicine 

The beating motion of cilia and flagella is driven by two rows of dynein arms: 

the outer dynein arms ODA and the inner dynein arms IDA. The ODAs are 

required to generate the normal beat frequency and the IDAs are responsible for 

the amplitude of the waveform. Although structural connections between the ODA 

and the IDA have been observed by cryoelectron tomography, the functional 

communication between the two species of the dynein arms has not been 

elucidated. In this study, we investigated the roles of the two intermediate chains 

IC1 and IC2 of Chlamydomonas ODA. We located the positions of the termini 

of the ICs within the ODAmicrotubule complexes by biotinstreptavidin labeling 

and cryoelection microscopy. It is noteworthy that the location of the Nterminus 

of IC2 is close to the previously observed ODAIDA linker. The biotintag added 

to the Nterminus of IC2 reduced the amplitude of the beating by half while the 

beat frequency was not decreased. These results suggest the Nterminus of IC2 

mediates the communication between the ODA and the IDA. 

P 

A novel protein complex required for the formation of microtubule 

square lattice in green tree frog sperm 

Toshiki Yagi 1 , Hiroshi Kubota 2 , Masahide Kikkawa 1 

1 

Grad. Sch. Medicine, Univ. Tokyo, 2 Grad. Sch. Science, Kyoto Univ. 

Fertilization of the green tree frog occurs in the viscous environment of a foam 

nest laid on the vegetation. The sperm cell moves through viscous media by 

spinning of the spiral head with flagellar bending movements. Previous EM 

analysis showed that the flagellum is composed of a pair of axonemes surrounded 

by a regular square lattice of hundreds of satellite microtubules MTs. To 

understand how the unique MT lattice structure forms, here we purified MT 

bundling proteins from the flagella and examined the properties of the proteins. 

Sperm cells were fragmented and fractionated into heads and short flagella by 

sonication and centrifugation. Proteins with MT bundling activity was purified 

from high salt extract of demembranated flagella by gel filtration chromatography. 

This fraction contained six proteins with the molecular size of 3545 kDa. All the 

proteins were precipitated together by MTpelleting assay, suggesting that they 

form a complex with MT. EM analysis on salt extracted sperm showed that the 

flagella lost crosslink structures in the MT lattice. Taken together, we suggest that 

the protein complex constitutes the crosslink structure required for the MT lattice 

formation. 

P 

 

 

 

 

 

 

LH/FSH 

 

 

LH/FSH 

TGN38 trans γtubulin 

 

 

cistrans 

 

 

 

 

 

 

 

P 

Mitochondria form continuous intracellular networkstructures: a 

study visualized by highresolution scanning electron microscopy 

Tomonori Naguro, Hironobu Nakane, Sumire Inaga 

Division of Genome Morphology, Fac. Medicine, Tottori Univ. 

The mitochondria of rat olfactorybulb granule cells in vivo cells within the 

tissue formed a continuous network of various shapes in each cell. On the basis 

of the morphological characteristics, these mitochondrial networks were roughly 

classified into four groups: Type1: Mitochondrial networks are composed of 

a single branched tubule of almost uniform thickness, 250300 nm. Type2: 

Distinguished by thickness, mitochondrial networks have two kinds of tubules: 

about 250350 nm and 100 nm across, respectively. Type3: Mitochondrial 

networks are composed of two parts; a globular part around 1.0 μm in diameter 

and a filamentous part about 45100 nm across. Type4: Highly complex 

mitochondrial networks consisting of elements those vary in shape. 

The significance of this morphological diversity of mitochondrial networks 

is discussed referring to recent studies with other means, notably light and 

transmissionelectron microscopy while considering the advantages and 

disadvantages of scanning electron microscopy methods employed for this here. 

P 

 

 

 

 

 

Mt 

C57BL/6 

1 5 

2 

2.5 

 

 

 

Mt Mt 

1 

Mt Mt 

5 Mt 

Mt 

 

Mt 

Mt 

Mt

154 

117 

P 

 

1 2 

1 

2 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

P 

- 

 

 

• • 

EGFR 

EGF 

EGFR 

EGFR 

EGFR - 

- 

AP1 GGA 

EGFR 

AP1 GGA2 

EGFR GGA1 

GGA3 

AP1 GGA2 

EGFR 

D EGFR GGA2 

 

CIMPR EGFR 

AP1•GGA2 EGFR EGFR 

 

P 

VAMP 

Khairani Astrid Feinisa 

 

 

SNARE 

VAMP 

vSNARE VAMP5 mRNA 

 

VAMP5 

VAMP5 

VAMP5 

 

 

 

 

VAMP5 

 

P 

 

 

1 2 3 3 

1 

2 3 

 

 

 

 

 

 

 

A23187: f6 μM Zymosan 

5 ms/frame 

AQUACOSMOS 

 

1 

47 

 

23 ms n=41 

18 ms n=21 

 

 

 

P 

Involvement of Rab in phagosome formation through regulating 

ARF activity by ACAP 

1 2 1 

1 

2 

Phagosome formation and subsequent maturation are complex sequences of 

events that involve actin cytoskeleton remodeling and membrane trafficking. In 

our livecell studies, we found that Rab35 accumulated markedly at the membrane 

where IgG opsonized erythrocytes IgGEs are bound. Rab35 silencing by RNA 

interference or the expression of GDP or GTPlocked Rab35 mutant drastically 

reduced the rate of phagocytosis of IgGEs. Actinmediated pseudopod extension 

to form phagocytic cups was disturbed by the Rab35 silencing or the expression 

of GDPRab35, although initial actin assembly at the IgGE binding sites was 

not inhibited. Furthermore, GTPRab35dependent recruitment of ACAP2, an 

ARF6 GAP, was shown in the phagocytic cup formation. The ARF6 activation 

during FcγRmediated phagocytosis was severely impaired by expressing GTP 

bound mutant of Rab35. Concomitantly, overexpression of ACAP2 along with 

GTPlocked Rab35 showed a synergistic inhibitory effect on phagocytosis. Taken 

together, it is likely that Rab35 regulates actindependent phagosome formation 

by recruiting ACAP2, which might control actin remodeling through ARF6. 

P 

AtgAmRNA HeLa /A study on 

AtgAmRNAknockdown HeLa cells 

 

 

 

Atg9 

 

Atg9A 9B 

Atg9A 

HeLa 

Atg9AmRNA siRNA 

lamp1 

 

 

EEA1 

 

 

 

Atg9A

117 155 

P 

mAtgAAcGFP mDFCPmCherry 

 

1 1 2 1 

1 

2 

 

Atg 

 

Atg9A 

Atg9A 

 

DFCP1 

Double FYVEdomain Containing Protein 1 

mAtg9A 

AcGFPmDFCP1mCherry 

mAtg9AAcGFP 

in vivo 

 

Atg9ADFCP1 

 

P 

Histological analysis of adipose tissues in Xpg null mice 

Hironobu Nakane 1 , Tadahiro Shiomi 2 , Toshio Kameie 1 , Sumire Inaga 1 , 

Tomonori Naguro 1 

1 

Div.Genome Morph., Fac. Med., Tottori Univ., 2 Rad. Safety Res. Center, Natl. 

Inst. Radiol. Sci. 

Some xeroderma pigmentosum group G XPG patients exhibit skin 

abnormalities, growth failure, lifeshortening and neurological dysfunctions, 

which are also characteristic features of Cockayne syndrome CS. The XPG 

gene XPG 

encodes a structurespecific DNA endonuclease that functions in 

nucleotide excision repair NER. The Xpg null mice showed postnatal growth 

failure, ataxia and premature death. We examined pathological changes of 

adipose tissues corresponding to clinical manifestations in the Xpg null mice, 

such as cachexia, joint contractures. Xpg null mice provide a good animal model 

for studying the mechanisms underlying clinical symptoms of XPG/CS, CS in 

humans. We will discuss the function of XPG protein in the adipose tissues of the 

Xpg null mice. 

P 

mdx 

1 1 2 1 

Khairani Astrid Feinisa 1 1 1 1 

1 

2 

 

 

 

 

 

 

 

 

 

 

mdx 

LC3p62 

P 

McARH 

ALP 

 

 

McARH 7777 

ALP 

ALP 

ALP 

1EEA1 

 

 

ALP ALP 

EEA1 ALP 

EEA1 ALP 

EEA1 EEA1 

 

ALP ALP 

15 

ALP 

McARH 7777 ALP 

EEA1 

 

ALP 

 

P 

 

 

 

focal adhesion, FA 

FA 

ECM 

 

ECM 

 

Cytograph, DNP• 10, 15, 30 μm 

FA Rho 

cSrc 

 

FA 

 

FA 

cSrc pY 418 

cSrc 

Rho 

 

cSrc Rho ECM 

 

P 

lamin A/C 

 

 

Type 

lamin A/C 

lamin A/C 

 

ROBC26 C26 lamin 

A/C 

C26 lamin A/C 

Dexamethasone, IBMX, insulin C26 

lamin A/C 

lamin A/C 

lamin A/C short hairpin 

RNA shRNA lamin A/C 

C26 

lamin A/C

156 

117 

P 

ABCG 

1 2 1 1 

1 

2 

 

5aminolevulinic acid ALA PDD 

PDT 

ALA protoporphyrin IX PpIX 

imatinib ATPbinding cassette ABC 

transporter G2 ABCG2 PpIX 

genistein ALA PpIX 

PDD PDT 

genistein ALA PpIX 

ABCG2 

ABCG2 Ko143 

genistein doxorubicin 

genistein 

PpIX 

PpIX ALAgenistein incubation 

 

genistein ALA PDD PDT 

 

P 

 

P 

DGKε 

 

 

DGK 

C DG 

DGKDGKε 

DGK / DG 

 

DGKε 

DGKε 

DGKε 

N DGKεNhalf 

DGKεKD 

DGKε ER tracker calreticulin 

DGKε 

DGKεNhalf 

DGKεKD 

DGKε N 

 

P 

DGKζ NAP DGKζ 

 

 

DGK C 

 

HeLa DNA 

Doxorubicin p53 

DGKζ 

p53 

 

MG132 p53 

 

 

DGKζ Nucleosome 

assembly protein NAP 1 NAP2 DGKζ 

HeLa DNA p53 

DGKζ 

NAP1/NAP2 p53 

NAP DGKζ p53 

 

P 

Cellular localization of ERα and their apoptotic effects 

 

 

The actions of estrogen have traditionally been thought to occur through binding 

estrogen receptors in nucleus. Recent data, however, support the idea that some 

of ERα in the cytoplasm of various target cells are localized in mitochondria. 

Moreover, ERα collaborates with a number of factors on cell membrane, in 

cytoplasm, and nucleus to effectively modulate transcription of distinctive 

groups of target genes. While some of these factors appear to regulate the 

chromatin configuration by controlling histone modifications at the promoter, 

others are members of various kinase cascades. In order to discern functions and 

interactions of ERα and its coregulators, we allocated ERα to different locations 

in ERαnegative Ishikawa cell, using permanent transfection technique. In this 

report the antiapoptotic effects were examined for ERα at different cellular 

locations in the presence of inhibitors for EGFR, PI3K, MEK, and p38MAPK. 

Cellular alterations in proliferation, membrane potential, and caspase activities 

were examined using immunohistochemistry, flow cytometry, and related 

techniques. The present results might shed light on different pathways of ERα 

signal transduction. 

P 

 

 

1,2 1 1 1,2 3 

2 1 

1 

2 2 2 

3 

 

 

 

 

 

 

 

 

 

PERKeIF2α Western blotting 

WST1 assay 

DNA ladder 

eIF2α 

eIF2α CHOP 

 

3% 

DNA

117 157 

P 

αMangostin 

1 2 3 1 

1 

2 

3 

HER2ER, PgR 

 

αMangostin 

 

MDAMB231 αMangostin 

Caspase 

c Realtime PCR 

 

 

Caspase389 

Caspase4 

c G1 S 

p21 Cip1 

PCNACyclin D1cdc25ACdK2 Cdk6 

Aktp38α ERK1/2 

αMangostin Akt 

 

p21 Cip1 G1 G1 

 

 

P 

Sevoflurane rat Per 

1,2 1 2 1 

1 

2 

 

Per2 suprachiasmatic 

nucleus: SCN 24 

mouse Per2 sevoflurane 

 

Ohe et al, 2010 SCN Per2 

 

sevoflurane SCN 

Wister rat Per2 rPer2 mRNA 

RI in situ hybridizasion rPer2 

Per2 

 

rPer2 

SCN rPer2 

25 rPer2 

sevofrurane 

SCN 

Per2 

P 

Transverse anchoring system of myofibril to sarcolemma: the 

morphological study 

Khairani Astrid Feinisa, Yuki Tajika, Maiko Takahashi, Hitoshi Ueno, 

Tohru Murakami, Hiroshi Yorifuji 

Grad.Sch.of Medicine Gunma Univ. 

Transverse anchoring system of myofibril to sarcolemma is subplasmalemmal 

cytoskeleton network that is anchored into the sarcolemma and provides an 

overall diffuse protection system of skeletal muscle. Three types of filament have 

been indicated as the transverse anchoring system; γ actin, desmin, and keratin. 

We have examined these subsarcolemmal cytoskeletal components in diaphragm 

and lower limb muscles of adult wild type and mdx mice by electron microscopy 

EM and immunohistochemistry. Isolated single fibers as well as small blocks 

of the muscles are used for both methods. EM revealed that the intermediate 

filaments seemed to either connect between the sarcolemma and the Zdisc and 

Mline or run as a part of subsarcolemmal density. These filamentous networks 

apparently decrease in mdx mice. These data suggest a morphological model of 

transverse anchoring system and how it affects the functions of skeletal muscle. 

P 

 

Khairani Astrid Feinisa 

 

 

 

 

 

 

 

 

SNARE 

 

2 SNARE 

 

SNARE 

 

 

P 

Prosaposin expression in cardiac muscle of mdx mice in early period 

of disease 

1 1 1 1 1 2 

1 

1 

2 

 

This study investigated the expression of the trophic factor prosaposin in the 

cardiac muscles of mdx mice. Prosaposin is strongly expressed in adult rat 

cardiac and skeletal muscles and plays a myotrophic role in vitro. However, few 

studies have focused on the role of prosaposin during muscle regeneration in mdx 

mice, which do not express the dystrophin gene. Dystrophin deficiency leads to 

progressive cardiac and skeletal muscle pathology. We examined the expression 

of prosaposin in the cardiac muscle in mdx and C57BL mice using HE staining, 

immunohistochemistry, and in situ hybridization in 4weekold mice. HE staining 

showed no obvious change in morphology of mdx cardiac muscles compared 

with control mice. The results of in situ hybridization showed that the main form 

of prosaposin mRNA is PS+0 in the cardiac muscle of mdx mice, and the mRNA 

expression is obviously lower in the cardiac muscles of mdx mice. Prosaposin 

protein also decreased in mdx cardiac muscle. These results suggest that the 

change of prosaposin expression happened earlier than morphological change in 

cardiac muscle of mdx mice. 

P 

 

 

 

 

14 

 

 

 

 

 

30%ST 

96 21 

34 56 58 42

158 

117 

P 

 

 

 

 

 

 

 

 

12 

Wistar n=18 

2 

ATPase pH 4.5 

Western blot 1 2 

 

 

1 

2 

 

2 

 

 

 

P 

 

 

 

 

 

dbdbdbm 

dbm dbm NDdbm dbm 

HPDdbdb dbdb NDdbdb dbdb HPD 

MF 

50 3 

dbm HPD dbdb HPD 

dbdb HPD 

dbm HPD 

dbm HPD 2 

dbdb 

HPD type1 

dbmND 

 

 

 

type 

P 

Thiel 

 

1 1 2 1,3 4 4 

4 2 5 4 

1 

2 2 3 

4 2 5 

Thiel Graz Dr. Thiel 

 

Thiel 

 

 

 

 

 

Thiel 5 8 

 

 

 

 

85.016.5 

mm81.712.3 mmThiel 

 

 

 

P 

ATDC Notch PCNA 

 

 

ATDC5 

Notch14 

 

 

Notch STK1 ATDC5 

60 STK3 

9 

Notch14 

PCNA 0 Notch14 

PCNA 70 

Notch 3 30 

5 Notch2, 3 

9 Notch 

 

Notch1 Notch4 PCNA 

Notch2 Notch3 

Notch23 

P 

Nox 

 

1 2 3 1 4 

1 

1 

2 

3 4 

 

 

Nox 

 

 

 

340 op/op 

4 

10%EDTA 

 

op/op 3 Nox4 

Nox1Noxa1Noxo1 

18 Nox4 

Nox1Noxa1Noxo1 3 

40 Nox1Noxa1Noxo1 

Nox4 

 

op/op Nox 

 

 

P 

 

1 1 1 2 

1 

2 

BFABP/FABP7 

 

4EDTA 

 

BFABP 

MAC2 

BFABP 

 

 

BFABP 

MAC2 

BFABP 

 

BFABP BFABP 

BFABP 

BFABP 

 

BFABP

117 159 

P 

 

1 1 1 1 2 

1 

2 

P 

 

1 2 

1 

2 

 

 

 

 

 

 

 

 

 

 

123 5 wistar 

 

 

 

 

1 

3 

 

 

 

 

 

7 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

P 

CT CT 

 

1 1 2 2 2 2 

1 

2 

 

CT 

 

,CT , 

, , 

2123 

35 CT CT DXA 

H CT N CT 

L CT 3 μX CT 

3 

μX CT H N L 

 

H 

99.55N 75.29L 50.34 

H 0.67GPaN 0.63GPaL 0.58GPa 

CT 

 

 

 

P 

 

1 2 1 2 2 

2 

1 

2 

 

 

232 

73 

 

1975 

1983232 

23 9.9 

70.3 58.3 

30 70 9 

1 

grade 4 

17.813 60.914 

9 5 

3 10 

 

 

 

 

 

P 

 

1,2 2 2 1 2 

1 

2 

P 

 

1 1 1 2 2 

1 

2 

 

 

 

12 3,8mm 

1 2 4 4 

 

CT 

 

 

SEMEDX 

Ca, P, C CT 

1 

2 4 

SEMEDX 2 4 

Ca P C 

 

 

 

 

 

 

 

 

 

 

 

5 wistar 

EX CO 4 7 

14 3 1 

 

 

 

 

7 14 

4 

7

160 

117 

P 

 

1 1 1 2 1 

1 

2 

 

 

 

 

 

 

 

 

/ +/+, +/,/+,/ 4 1 1 

30 10 

CL TC 

 

+/+ /+ / 

2 

 

 

 

 

P 

 

 

1 2 3 4 

1 

2 3 

4 

 

 

 

 

 

 

 

 

 

 

 

 

Softex 

 

 

 

90 

 

 

P 

Klotho 

1 1 1 2,3 4 

1 

1 

2 

3 4 

 

 

klotho klotho/ 

 

5 klotho/ 

von Kossa, van Geison I MGPαsmooth 

muscle actinα SMAALPENPP1 

TEM MGP 

klotho/ α SMA 

MGP I 

 

ALP ENPP1 

TEM 

MGP 

klotho/ 

 

 

 

P 

FAMA 

1 1 1 1 1 2 

2 1 

1 

2 

FAM20A is a member of family with sequence similarity 20 FAM20 which 

has three members FAM20A, FAM20B and FAM20C in mammals. We have 

reported a transgenic Tg mouse line the lineage 230 exhibiting growth disorder 

associated with a 58kb fragment deletion in chromosome 11E1 that encompasses 

part of the FAM20A gene. Recently, some studies have reported that patients 

with amelogenesis imperfecta caused by FAM20A mutation display several 

dental phenotypes including hypoplastic enamel, failure of tooth development 

and gingival hyperplasia. In the present study, we aimed to clarify how the loss of 

FAM20A protein influenced the development of mouse dental tissues. We have 

observed delayed tooth eruption in the homozygous mice of the lineage 230. 

Immunohistochemistry indicated that FAM20a protein was expressed in osteoid 

tissue and tooth germ of normal mice of the lineage 230, but was not expressed 

in homozygous mice of the lineage 230. The light microscopy demonstrated 

hypoplastic enamel and gingival hyperplasia in the homozygous mice of the 

lineage 230. In conclusion, this study revealed that the FAM20A protein played a 

critical role in enamel biomineralization. 

P 

GABA GABA 

1 2 2 2 3 

3 4 1 

1 

2 

3 4 

 

 

 

GABA 

GAD 

GABA 

GABA GABA 

GABA 

 

OUMS27 

RTPCR GABA GABA 

 

RTPCR GABA GABAA 

α2α5β1 GABAB 

GABAB1 

GABAB2 mRNA GABAB2 

mRNA 

 

GABAB GABAB 

 

P 

Polypterus senegalus 

Oxydoras niger 

1 1 1 1 2 

1 

1 

2 

5 

 

Polypterus senegalus Oxydoras niger 

 

SGCs 

 

 

 

SGCs 

SGCs 

 

50 μm 

100 μm 

SGCs 

 

SGCs 

SGCs 

SGCs

117 161 

P 

planar cell polarity 

 

1 2 

1 

2 RI 

 

2 

 

 


4-5 Wistar 

EDTA 

frizzled3 Vangl2 Alexa488 Alexa555 

 

 

β N 

Vangl2 

 

frizzled3 

 


 

P 

 

Miyuki Yamamoto, Shoichi Iseki 

 

 

 

SD GCT 

GCT SD ID 

stem or progenitor cell 

5 K5 

progenitor cell basal 

epithelial cell K5 

K5 

progenitor cell 

C57BL/6 K5 

stem cell 

 

 

ID ID 

lumen 

progenitor cell 

 

P 

Studies on the submandibular gland of androgen receptordeficient mice 

Kannika Adthapanyawanich, Hiroki Nakata, Tomohiko Wakayama, 

Shoichi Iseki 

Department of Histology and Embryology, Graduate School of Medical Science, 

Kanazawa University 

In the submandibular gland SG of mice, the duct portion called granular 

convoluted tubule GCT is developed preferentially in the male. To clarify if 

the androgendependent differentiation of GCT cells is mediated by the classical 

androgen receptor AR, we examined SG in the C57BL/6 strain mouse deficient 

for AR ARKO. The ARKO mouse was generated by the Cre/loxP recombination 

system using the floxedAR mouse provided by Dr. Shigeaki Kato. In male ARKO, 

GCT was not formed in the duct system during the postnatal development, 

resulting in the femaletype SG at adult age 8W. No abnormality was recognized 

in development of the acinar system. The levels of the expression of NGF and 

EGF, markers of GCT cells, in SG of ARKO male were even lower than those 

of wildtype female. When testosterone was administered to ARKO male and 

wildtype female, the duct system of wildtype female was converted to the male 

type both morphologically and in the levels of marker gene expression, whereas 

that of ARKO male remained in the female type. These results confirmed that 

the androgendependent cell differentiation in the duct system of mouse SG is 

mediated by the classical AR. 

P 

 

1 2 3 

1 

2 NPO 

3 

 

 

Neovison vison 

 

 

HE 3.5NHCl 

S800 

45 V 

 

1 

 

 

 

1 

1 

 

 

 

 

 

P 

 

 

 

 

 

7 

 

7 1 

deoxycholate 

 

 

 

 

deoxycholate 

 

STIM stromal interaction molecule 

SOCEstoreoperated calcium entry 

SOCE 

 

P 

A AB 

 

 

 

3 

 

3A 

3AB 

3A 3AB 

SN38 

 

3A 3AB

162 

117 

P 

 

 

 

IVCT FS 

IgA Ig 

 

-193 IVCT 

2% PFA 

FS 

2%PFA 2%PFA - 

- PFDH 

- HE IgA 

IgG1 Ig κ Lκ IgM IVCT 

HE 

IgA IgLκ 

IgG1 IgM 

IgG1 

PFDH IgA 

IVCTFS 

 

P 

M Epidermal type FABP EFABP/FABP 

 

1 2 1 

1 

2 

Fatty acid binding protein FABP 

 

Epidermal type FABP EFABP/FABP5 

M 

Histochem Cell Biol. vol.1326, 2009 M 

17 18 

116 M 

16, 17, 18, 19 

IgA IgA 

EFABP 

M EFABP 

EFABP 

 

galectin4 

M EFABP galectin4 

 

P 

A 

1 2 1 1 1 

1 2 1 

1 

2 

 

Lampreys are ancestral representatives of vertebrates known as jawless fish. The 

Japanese lamprey, Lethenteron japonicum, is a parasitic member of the lampreys 

known to store large amounts of vitamin A within its body. How this storage is 

achieved, however, is wholly unknown. Within the body, the absorption, transfer 

and metabolism of vitamin A are regulated by a family of proteins called retinoid 

binding proteins. Here we have cloned a cDNA for cellular retinolbinding 

protein CRBP from the Japanese lamprey, and phylogenetic analysis suggests 

that lamprey CRBP is an ancestor of both CRBP I and II. The lamprey CRBP 

protein was expressed in bacteria and purified. Binding of the lamprey CRBP to 

retinol Kd of 13.2 nM was identified by fluorimetric titration. However, results 

obtained with the protein fluorescence quenching technique indicated that lamprey 

CRBP does not bind to retinal. Northern blot analysis showed that lamprey CRBP 

mRNA was ubiquitously expressed, although expression was most abundant in the 

intestine. Together, these results suggest that lamprey CRBP has an important role 

in absorbing vitamin A from the blood of host animals. 

P 

cKIT 

 

 

 

 

ICC 

ICC 

cKIT 

 

ICC 

αSMA cKIT 

αSMA E11.5 

12 cKIT E11.5 

E12.5 

cKIT αSMA E13.5 cKIT 

αSMA E15.5 αSMA 

cKIT 

 

αSMA E18.5 

E14.5 E16.5 αSMA 

cKIT 

αSMAcKIT 

 

P 

ICCSP 

 

 

 

Interstitial cells of Cajal: ICC 

 

Subserosa: SS ICC ICCSS 

Submucosal Plexus: SP ICC ICCSP 

ICCSP 

ICC 

 

 

 

 

 

ICCSP 

 

 

P 

CCK 

 

 

CCK 

CCK1 

CCK1 

 

CCK1 

in situ hybridization 

 

CCK1 

 

CCK 

 

G 

 

 

CCK1 

CCK

117 163 

P 

The metabolism of cholesterol after bile duct degeneration in lamprey 

Mayako Morii 1 , Yoshihiro Mezaki 2 , Noriko Yamaguchi 2 , Kiwamu Yoshikawa 2 , 

Mitsutaka Miura 2 , Katsuyuki Imai 2 , Taku Hebiguchi 1 , Ryo Watanabe 1 , 

Hiroaki Yoshino 1 , Haruki Senoo 2 

1 

Department of Pediatric Surgery, Akita University Graduate School of Medicine, 

2 

Department of Cell Biology and Morphology, Akita University Graduate School 

of Medicine 

Lampreys are unique vertebrates in that they lose biliary trees entirely during 

the metamorphosis. Despite the biliary atresia, lampreys do not develop neither 

biliary cirrhosis nor liver dysfunction. In other vertebrates, obstruction of bile 

ducts results in fatality because of the cytotoxicity of bile salts. It means that 

lampreys have evolved to use another metabolic pathway of bile juice in order 

to evade the cholestasis. However, molecular mechanism of this pathway is still 

unknown. The aim of this study is to investigate the way how the lamprey avoid 

the pathological consequence of cholestasis. We have cloned the cDNA sequences 

of two kinds of P450, which show high degree of homology with mammalian 

CYP7A1 and CYP27A1, respectively. We are now evaluating the expression 

levels of the mRNA for these genes in liver, gonad, gill, heart and intestine along 

with the temporal expression profiles from larva to adult. The present study 

suggests how and where bile acids are detoxified in adult lampreys. Understanding 

how lampreys avoid cholestasis could be valuable for progress in the treatment of 

human obstructive cholangiopathy. 

P 

NAFLD 

 

1 1 1 2 1 

1 

2 

 

 

NAFLD NAFLD 

NASH NAFLD 

NASH 

9 C57BL/6 MCD 

30 M R MCD 8 16 

30 2 AST, ALT 

 

M 2 AST, ALT 

NAFLD 

10 

M 16 CD68 

CD68 

R 3 

R 16 

MCD 16 CD68 

30 

CD68 

 

P 

Deltalike 

 

 

 

 

Deltalike3 DLL3 

DLL3 

Huh2 DLL3 

 

AnexinV TUNEL singlestrand DNA 

DLL3 Huh2 

DLL3 binding 

assay DLL3 Small G Cdc42 

CRIB 

DLL3 HuH2 Cdc42 

PAK1 MAP kinase 

DLL3 

 

DLL3 Cdc42 PAK1 MAP kinase 

 

P 

 

1 2 2 2 1 

1 

2 

 

 

CLD 

 

 

 

 

ICR 85O 2 

RA21Air 14 

RA 7 RA14 Air14d 

14 O 2 14dRA 21 Air21dO 2 Air21d 

4%PFA 

 

O 2 14d 

O 2 

Air21d 

 

 

CLD 

 

P 

CEACAM 

1 Nabil Eid 1 2 1 1 

1 

2 

 

prox1 

 

Carcinoembryonic cell adhesion molecule1 CEACAM1 

CEACAM1 

VEGFC 

BJMC338 VEGFC 

BJMC3879 

2 CEACAM1, 

VEGFR3VEGFR3 

phosphotyrosine 

VEGFA 

BJMC3879 

CEACAM1 BJMC3879 

CEACAM1 BJMC338 

BJMC3879 

BJMC3879 VEGFR3 

BJMC3879 CEACAM1 

VEGFC 

 

P 

The role of peritoneal lymphatic system on the human peritoneal 

dissemination 

Masahiro Miura 1 , Yutaka Yonemura 2 , Yoshio Endou 3 

1 

Department of Human Anatomy, Faculty of Medicine, Oita University, 2 NPO 

organization to support peritoneal dissemination treatment, 3 Department of 

Molecular Targeting Therapy, Kanazawa University 

The role of lymphatic microenvironment on cancer metastasis was studied. 

VEGFCgene transfectants of the gastric cancer cell lines were inoculated into 

the peritoneal cavity of nude mice. The tissue samples from human disseminated 

peritoneum were stained with 5,NaseALPase double staining and aniti 

VEGFR3 immunostaining. Mouse VEGFR3 gene expression was induced after 

inoculation. In the early peritoneal dissemination PD stage, newly lymphatic 

networks LN were detected in the periphery of the primary tumor. The main 

source of lymphatic dissemination may originate from the peritumoral LN. In the 

PD, three patterns of tumor lymphangiogenesis, namely centrifugal, centripetaland 

combination growth patterns were found. Free cancer cells invaded the numerous 

blind endings that came from extended subperitoneal LN. In the late PD stage, 

the number of LN decreased due to the destruction of cancer cells. In SEM 

observation, Macula cribriformis were seen in the diaphragmatic subperitoneal 

tissue layer and Morrison pouch. Lymphangioginesis including newly formed LN, 

induced by VEGFC is closely associated in formation of PD and lymph node 

metastasis mainly via the prelymphatic channels.

164 

117 

P 

 

 

 

 

 

 

 

α β 2 

α 18 β 8 24 

 

 

3 

 

α 

β 

Zeiss LSM710 

β18 

β5 β5 2 

αv 

 

P 

 

 

 

66 CD41 

 

 

 

ICR 

 

CD41 

12 22.96.6/mm 3 14 

38.410.0/mm 3 16 

0 21.710.0/mm 3 6 3.62.0/mm 3 20 

 

 

 

 

 

2 

 

P 

The role of hematopoietic factors and Wnt signaling during tooth 

development 

Masataka Sunohara, Iwao Sato 

Department of Anatomy, School of Life Dentistry at Tokyo, The Nippon Dental 

University, Tokyo, Japan 

Purpose:It has been reported that Wnt signaling pathway is involved in the early 

tooth development and regulates haematopoietic stem cells HSCs. However 

the role of hematopoietic factors during tooth development remain unclear. 

Here we examine the role of hematopoietic factors and Wnt signaling during 

tooth development in mice. Methods:We performed in situ hybridization and 

immunohistochemically stained serial sections of dental tissues with antibodies 

against hematopoietic factors and Wnt. 

Results:We observed that hematopoietic factors were detected at the some stages 

of tooth development and Wnt was localized in mesenchymal layers at the late 

stage of tooth development. Conclusions:These data suggest that hematopoietic 

factors and Wnt were involved in tooth germ development in mice. 

*This study was was supported by a GrantinAid for Scientific Research C 

No.22592052. 

P 

 

1 2 2 2 

1 

2 

 

 

Al V 64 

Ti6Al4V PMN 

PMN NADPH oxidase 

O2 H2O2 

PMN 

OPZ OPZ H2O2 

Ce V 

PMN Al PMN 

PMN 

Ti6Al4V PMN H2O2 

 

PMN Ti6Al4V PMN 

3710 Ce H2O2 

3720 GA 

 

PMN PMN H2O2 

P 

podoplanin 

 

 

 

 

 

C57BL/6 4% 

 

podoplaninLYVE1 

in situ hybridization 

CCL21 

 

5 HE 

3 

podoplanin 

LYVE1 podoplnanin 

3 5 CCL21 in 

situ hybridization 

CCL21 podoplanin podoplanin 

 

 

P 

 

 

 

 

 

 

 

 

VEGFA 

 

 

VEGFA 

 

PDGFRβ NG2 NG2 

PDGFRβ NG2

117 165 

P 

 

1 1 2 2 1 

1 

2 

 

 

DDS 

 

 

 

 

 

SCID 1 

CDDP 3 

 

 

 

 

CDDP 1 

 

 

 

 

 

P 

 

1 1 2 3 3 1 

1 

2 3 

 

 

 

5 8 

 

 

 

10 

 

 

 

1 

1 4-7 2 

3-7 3 3-7 

 

1 

7-12 2 6-14 3 

5-11 

 

 

 

P 

 

 

 

64 128 

 

124 97 

94 73 

46 36 27 

21% 1 1% 

 

 

IVI 6 IIV 

N 

I II 

IIIIV III 

V 

VI 

 

 

 

 

 

P 

 

 

1 1 2 1 1 

1 1 

1 

2 

 

 

 

 

Wistar 

 

 

SDSPAGE 

50 nm 

 

 

 

 

 

 

SDSPAGE 

 

 

P 

Nkx. 

1 1 1 1 2 

1 

1 

2 

 

Nkx2.5 

GATA4 TBX5 

Nkx2.5 

 

KONkx2.5+/− 

 

Nkx2.5+/− 

 

1020 C57BL/6JNkx2.5+/− 

4%PFA 

 

 

Nkx2.5+/− 

 

 

 

P 

Morphological analysis of effects with arachidonic acid in smooth 

muscle cells 

Hideyuki Tanaka 1 , Haruo Hagiwara 2 , Shinnichi Mitui 1 

1 

Labo.Sci, Gunma Univ. Grad. Sch. Health Sci., 2 Anatomy, Teikyo Univ. Sch. Med. 

We previously reported that arachidonic acid enhanced the ATPase activity 

of smooth muscle myosin of which 20 kDa myosin light chain MLC20 was 

phosphorylated to leave no room for the additional phosphorylation. We report 

here that configuration of smooth muscle myosin changes to activated form 

with arachidonic acid from inactiveted form under unphosphorytated condition. 

And, selfaseembly of each myosins was enhanced in vitro. Such a direct effect 

of arahidonic acid was confirmed by inducing superprecipitation, an invitro 

contraction, and contraction of TritonX skinned fiber of smooth muscle under 

conditions that the phosphorylation of MLC20 didi not proceed. An electron 

microscopic observation of superprecipitation, and of skinned fibers with 

arachidonic acid indicated an enhanced association of actin fibers with myosin 

filaments.

166 

117 

P 

FABP 

 

 

 

 

FABP7 

Kupffer KC 

KC FABP7 

FABP7 KC 

FABP7 clodoronateliposome clo 

lipo KC FABP7 

clolipo 7 F4/80+KC 

FABP7 

clolipo 7 F4/80+KC 78% FABP7+ 

FABP7 J774 FABP7/J774LPS 

TNFα TNFα 

IκB 

FABP7 FABP7 

KC 

 

P 

 

1,3 Randeep Rakwal 1 1 1 1 

1 1 2 3 1 

1 

2 3 NPO 

 

 

 

 

 

 

 

1/3 

1.2% 5 / 1.2%NP 4 

6 LPS 10 mg/ 

kg mRNA DyeSwap 

DNA 1.2%NP 5000 

 

 

1000 

 

 

 

 

P 

Functional Analysis of Nucleoprotein diet from salmon testis, Shirako 

via Intestinal IgA secretion and TLR stimulation 

1 Randeep Rakwal 1 1 1 1 

1 1 2 3 1 1 

1 

2 3 NPO 

 

Salmon testis, Shirako contains in plenty nucleic acids and protamin protein, 

termed as Nucleoprotein NP. However, we know little about their functions in 

the human body, and molecular evidence for these potential beneficial effects. 

Therefore, the aim of this study was to elucidate the effects of dietary NP and 

know molecular evidence for these potential beneficial effects. 

First, we analyzed the innate immunity response in mice by measuring IgA 

secretion in feces. To do so, mice were fed high or low level NP diets for 4 weeks. 

Fecal IgA level was measured with an ELISA kit. Our results revealed that the 

fecal level of IgA was little higher in the highNP diets group than in the lowNP 

diets group. 

Next, we examined the human TLR9 stimulation and cytokine production 

by effect of NP. We added NP into HEK293 cells overexpressed human TLR 

9 to measure NFκB transcriptional activity and, PMA induced U937 cells to 

measure several cytokine expressions. The results show that NP affects on TLR9 

stimulation and some cytokine expressions. 

Here, we show some evidences and hypothesize that NP diet might affect on some 

cytokine expressions to increase mucosal immunity. 

P 

The effects of adjuvants on autoimmune responses against testicular 

antigens in mice 

Musha Muhetaerjiang, Shuichi Hirai, Munekazu Naito, Hayato Terayama, 

Qu Ning, Masahiro Itoh 

 

A subcutaneous injection with testicular homogenate TH can easily induce 

systemic immune responses against the autoantigens of germ cells. Experimental 

autoimmune orchitis EAO is pathologically characterized by lymphocytic 

inflammation accompanied by the spermatogenic disturbance. Classically, the 

murine EAO is induced by immunization with TH + Complete Freund’s Adjuvant 

+ Bordetella Pertussigens, and it has been considered that these adjuvants is 

needed to enhance the immune responses. However, there remains a possibility 

that adjuvants affect autoimmune responses against the autoantigens without 

TH. In the present study, we examined this possibility using real time RTPCR, 

Western Blotting and immunohistochemical staining. The results demonstrated 

that testicular histopathological state and expressions of intratesticular cytokines 

were normal in mice injected with adjuvants alone. However, various kinds of 

serum autoantibodies with not only germ cells but also Sertoli cells were detected 

in the adjuvant injected mice. These results indicate that adjuvants alone can 

evoke testicular autoimmune reactions against various autoantigens irrespective of 

no use of TH. 

P 

 

 

 

 

 

 

 

 

 

 

 

 

 

2 

 

 

 

 

P 

Germ cell death in testicular autoimmunity 

Kuerban Maimaiti, Shuichi Hirai, Hayato Terayama, Qu Ning, Yuki Ogawa, 

Musha Muhetaerjiang, Munekazu Naito, Masahiro Itoh 

 

Experimental autoimmune orchitis EAO is characterized by T celldependent 

lymphocytic inflammation and damage to seminiferous tubules, causing death of 

testicular germ cell TGC. The aim of the present study is to investigate the role 

of apoptosis systems in the TGC death in EAO in mice, using real time RTPCR 

and immunostaining. The results showed that the many Tdt mediated dUTP nick 

end labeling positive TGC were found at the active EAO stage and persistently 

observed in the seminiferous epithelium until the postactive EAO stage. Intra 

testicular mRNAs expression of both Fas and Bax increased at the active EAO 

stage and dramatically decreased at the postactive EAO stage. In contrast, intra 

testicular the mRNAs expression of both FasL and Bcl2 did not show significant 

changes at the active EAO stage but extremely increased at the postactive EAO 

stage. Immunohistochemically, some Fas or Baxpositive TGC were detected at 

the active EAO stage and hardly found at the postactive EAO stage. In contrast, 

some FasL or Bcl2 positive TGC were found at the active EAO stage but many 

of them were observed at the postactive EAO stage.

117 167 

P 

 

1 2 1 3 1 

1 

2 

3 

 

 

A,B,C D 

 

 

 

 

 

B PNAS, Yuan et al. 2007 

 

 

152 

 

 

 

CD68S100 

Fascin 

 

 

P 

 

 

 

1 

 

 

conduit 

 

ICR 

2000 kDa 

10 kDa 310 4 PFA 

3 530 

OCT 10 μm 

Tris ER 

TR7 TypeIV 

5 nm 

54 nm 

 

 

 

 

 

P 

trafficking 

 

 

secondary lymphoid organ, SLO 

traffickingT 

SLO 

 

T DC 

DC 

 

T T 

trafficking molecule 

DC trafficking 

DC 

DC 

 

 

T DC trafficking SLO 

P 

Alterations of synaptic transcytosis induced by ethanol exposure: 

apocrinelike structure in the rat 

Tomiko Yakura 1 , Takanori Miki 1 , JunQian Liu 1 , Kenichi Ohta 1 , 

Katsuhiko Warita 1 , Yoshiki Matsumoto 2 , Shingo Suzuki 1 , Motoki Tamai 1 , 

Yoshiki Takeuchi 1 

1 

Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa 

University, 2 Laboratory of Animal Science, Faculty of Agriculture, Kagawa 

University 

Effects of alcohol exposure on synaptic structure were investigated in the NST 

in the rat. Ethanolfed animals were allowed free access to liquid diet containing 

ethanol for 3 weeks. A few terminals were characterized by deep indentation 

of axodendritic membranes into the postsynaptic neurons which was similar to 

the apocrine structure. Other animals of ethanol exposure received injection of 

WGAHRP into the vagus nerve and were prepared for electron microscopy. 

HRPreaction product RP was recognized easily as electron dense lysosomal 

substance when lead citrate staining was omitted. The terminals containing HRP 

RP also revealed quite similar structure to indentation of axodendritic membranes 

described above. The results are considered to confirm that terminals forming 

“apocrine-like structure” are originated from afferent fibers of the vagus nerve. 

The present study raised the possibility that remarkable alteration of the synaptic 

structure induced by ethanol exposure leads neuronal transcytosis of materials 

including proteins which is absolutely different from vesicular exocytosis involved 

in chemical synaptic transmission. 

P 

LAMP 

 

 

Lysosomeassociated membrane protein2 LAMP2 

LAMP2 

X 

 

 

 

LAMP2 

12 LAMP2 

LAMP1 D 

 

A 

GM130 

 

LC3 LAMP2 

 

LAMP2 

 

P 

Synergistic interaction between Golgi outposts and RNA granules in 

postlocal translational secretory pathway 

Souichi Oe, Yasuko Noda 

Jichi Med. Univ. Tochigi, Japan 

In the central nervous system, neurons have Golgi outposts in dendrites and 

it has been shown that its subcellular distribution and morphology are related 

with various aspects of cellular event. In this study, we analyzed the subcellular 

localization of Golgi outposts under the various stimulations in cultured 

hippocampal neurons. In normal conditions, Golgi outposts localized in basal 

dendrites and contained some Golgi matrix proteins including GM130, GRASP65 

and BicaudalD2. When the neurons were cultured with several stimulations, the 

distribution of Golgi outposts were enhanced towards the distal dendrites. This 

observation was similar to the dynamics of RNA granules, and in fact, Golgi 

outposts colocalized with CPEB which is the translational repressor and the 

component of the RNA granules. Furthermore, we constructed the GFP based 

reporter plasmid that has the signal sequence and the dendritic localization signal 

of CaMKII mRNA and observed the localization in dendrites with or without the 

stimulations. These results suggest that Golgi outposts act synergistically with 

RNA granules and contribute to the postlocal translational secretory pathway in 

dendrites.

168 

117 

P 

ARF BRAG/IQSEC 

 

 

Brefeldin Aresistant ArfGEF 2 BRAG2/IQSEC1 

ADP 6 Arf6 

GEF 

BRAG2 AMPA 

 

BRAG2 

BRAG2 

BRAG2pan C 

BRAG2LC BRAG2 

 

BRAG2LC 

PSD AMPA 

BRAG2pan PSD 

 

BRAG2 PSD 

 

BRAG2Arf6 AMPA 

 

P 

Arf EFAA 

 

 

GTP ADP 6 ARF6 

 

 

ARF6 GEF 

EFA6A 

 

EFA6A 

 

EFA6A 

PSD95 

 

 

EFA6A 

 

EFA6A PSD PSD 

EFA6A 

PSD PSD ARF6 

 

P 

Roles of BMP signaling in synapse development 

 

 

Continual formation and elimination of synapses is required for refinement 

of the neuronal circuit. Synapse remodeling should be regulated by specific 

signaling mechanisms, but few molecules involved in synapse elimination have 

been identified, despite a large body of work on molecular cascades that initiate 

or enhance synapse formation. Here, we report that BMP4 negatively regulates 

hippocampal synapse formation in vitro. First, we confirmed that hippocampal 

neurons respond to BMP4 by measuring accumulation of activated Smad by 

immunocytochemistry. Next, we constructed BMP4EGFP and visualized its 

localization in living hippocampal neurons. Timelapse fluorescence imaging 

revealed that BMP4EGFP clusters showed bidirectional movements in the 

somatodendritic compartment. Finally, overexpression of BMP4 in neurons 

induced reduction of both density and immunoreactivity of VGluT1positive 

presynaptic puncta associated with BMP4expressing dendrites. These results 

indicate the presence of transsynaptic retrograde signaling in BMP4dependent 

suppression of synapse formation. 

P 

Roles of ACF, a large linker protein interacting with both 

microtubules and Factin, in the postsynaptic functions 

 

 

Two major components of the cytoskeletal system, Factin and microtubules MTs, 

have been shown to be involved in the postsynaptic functions. Actin network 

regulates the spine shape and motility. MTs invade into spines intermittently and 

help spine head expansion through the remodeling of actin network. Although 

these observations suggest importance of coordination between two cytoskeletal 

systems, there have been few reports on molecular mechanisms that regulate their 

interaction. 

ACF7/MACF1 is a member of spectraplakin family protein and links between 

Factin and MTs. By manipulating ACF7 content in cultured hippocampal 

neurons, we assessed the impact of ACF7dependent cytoskeletal reorganization 

on spine morphology. First, overexpression of ACF7GFP revealed accumulation 

of ACF7 in a subset of dendritic spines. This selective targeting may reflect 

specific functional states of spines. Second, downregulation of ACF7 by shRNA 

induced elongation of spines, which was rescued by expression of shRNA 

registant ACF7 cDNA. This phenotype may indicate that reduction of ACF7 

blocks MTdependent spine head expansion and reversibly enhances excess 

growth of thin spines. 

P 

Imaging dynamics of axonal mitochondria 

 

 

The complex geometry of neuron requires specialized mechanism to allocate 

sufficient number of organelle to neurites and synapses. Proper distribution 

of mitochondria is critical for multiple neuronal functions including energy 

production, calcium homeostasis, neuronal apoptosis, synaptic transmission and 

plasticity. However, mitochondrial dynamics underlying the maintenance of 

their proper distribution is largely unknown. To study mitochondrial dynamics in 

neurons, we established a live imaging system to visualize neuronal mitochondria 

in hippocampal dissociated culture. We are now investigating neuronal 

mitochondria distribution and trafficking by expressing mitochondrial outer 

membrane protein Omp25C tagged with fluorescent proteins and performing time 

lapse confocal imaging. The size, distribution and motility of mitochondria in the 

axon and synaptic contact sites are analyzed quantitatively and these data will be 

integrated to estimate the rate and extent of mitochondria replacement in specific 

neuronal compartments. 

P 

ERRγ 

 

 

estrogenrelated receptor: ERR 

 

estrogen receptor: ERαßγ 3 

ERR 

DNA ER ERR 

ER 

ER ERR 

3 ERRγR 

10 ERRγR 

 

 

 

ER 

ERRγR 

ER 

ERRγR ER

117 169 

P 

Agerelated changes in estrogen receptorβ mRNA expression in 

male rat brain 

 

 

Estrogen plays important roles not only in reproductive function but also in other 

functions such as cognition and emotion. Estrogen acts mainly via its receptor 

ER, ERα and β, in target tissues. During aging, estrogenic actions are altered 

in both females and males, raising the possibility that expression level of ER may 

be altered with age. Agerelated changes in ER expression in female rat brain 

have been well demonstrated with regard to reproductive aging, while very little 

is known about the effects of age on the expression of ERs, especially ERβ, in 

males. In this study, to elucidate the effects of aging on ERβ expression in the 

male brain at the transcriptional level, we performed in situ hybridization using 

young, middleaged and old male rats. We revealed the number of ERβ mRNA 

positive cells was decreased with age in the cerebral cortex, hippocampus, 

dorsal endopiriform nucleus, medial septal nucleus, amygdala, anteroventral 

periventricular nucleus, substantia nigra, raphe and locus coeruleus. These 

results suggest that ERβ expression in male rat brain decreases with age at the 

transcriptional level and that these aging effects are regionspecific. 

P 

PRLR 

 

1,2 2 2 1 2 

1 

2 

 

ARC 

PRLR PRLR 

 

 

TH 

PRLR 

 

PRLR 

ARC TH PRLR 

 

TH PRLR 

PRLR 

ARC 

PRL ARC 

PRLR PRL 

PRLR 

 

P 

 

 

 

 

 

 

 

RTPCR 2 Wistar 

OVX Sham 1 

2 

17β 

1 2 17α/C1720 

1 

OVX 2 OVX 

Sham 

1 2 

 

 

 

P 

Sporadically lurking HAPimmunoreactive cells in the hippocampus 

and their morphological relation with steroid receptors 

Md. Nabiul Islam, Ryutaro Fujinaga, Akie Yanai, Mir Rubayet Jahan, 

Yukio Takeshita, Keiji Kokubu, Koh Shinoda 

Division of Neuroanatomy, Yamaguchi University Graduate School of Medicine 

Huntingtinassociated protein 1 HAP1 is a neural huntingtin interactor that is 

widely expressed in the limbic and hypothalamic regions. Although HAP1 has 

been reported to be associated with steroid receptors, HAP1immunoreactive 

HAP1ir cells remain to be identified in the hippocampus. We determined the 

distribution of hippocampal HAP1ir cells in light and fluorescence microscopy 

and characterized their morphological relationships with steroid receptors, 

markers of adult neurogenesis and the GABAergic system in adult Wistar rats 

of both sexes. HAP1ir cells that were sporadically distributed in the subgranular 

zone SGZ of the dentate gyrus and in the interface between the stratum 

lacunosummoleculare and stratum radiatum of Ammon’s horn, were identified as 

the “sporadically lurking HAP1-ir (SLH)” cells. The SLH cells showed no clear 

association with the markers of adult neurogenesis in the SGZ, while all the SLH 

cells expressed neuN. More than 90% of the SLH cells expressed nuclear ERα, 

while more than 65% of them exhibited GABA immunoreactivity. We conclude 

that SLH cells might be involved in estrogendependent hippocampal functions 

through ERα and GABAergic regulation. 

P 

GR 

 

 

 

 

GR 

GR 

 

GR 

vimentin GR 

GR GR 

coactivator p300 SRC1 

ADX 

GR coactivator 

vimentin 

GR vimentin p300 

SRC1 ADX vimentin 

GR 

p300SRC1 

GR 

GR 

 

P 

 

1 1 2 1 

1 

2 

24 

 

 

 

cfos, Per1 

cfos, Per1 

gate 

 

 

 

 

10 

1 3 2 30 

cfos Per1 

in situ hybridization

170 

117 

P 

GALP 

1 1 1,2 1 

1 

2 

GALP 

GALP 

 

 

GALP GALP 

24 

GALP 

GALP 

GALP 

GALP 

4510 1216 

 

GALP 

PEPCK SREBP1 

GALP 

GALP 

GALP 

GALP 

P 

CGRP 

 

 

 

CGRP 

 

CGRP jugular ganglion 

 

Fluorogold 

 

nodose ganglion Fluorogold CGRP 

Fluorogold 

jugular ganglion Fluorogold 40% CGRP 

jugular ganglion nodose ganglion 

Fluorogold 20% 

Fluorogold CGRP 

2% 

CGRP 

 

CGRP 

 

P 

SSRI 

 

 

 

 

 

SSRI 

 

SSRI cAMP 1 transforming 

growth factor beta TGFβ 

2 cAMP 

cAMP response element binding protein CREB 

brainderived neurotrophic factor: BDNF 

 

 

 

SSRI 2 4 

 

 

SSRI 

P 

 

 

1 1 1 1 2 3 

2 1 

1 

2 3 

 

MSCs 

1989 

PACAP 

MSCs PACAP 

MSCshMSCs 

PACAP SCI 

 

C57/BL6 9/10 SCI 

10/11 50 /μLμ 

hMSCs 

SCI 

PACAP PCR 

SCI 7 hMSCs 

 

SCI PACAP 7 

hMSCs 

PACAP PACAP 

hMSCs hMSCs 

 

hMSCs PACAP 

 

P 

 

1,2 1 1 3 1 

1 

2 3 NPO 

 

PD 

PD 

 

 

2 

1.2% 7 

20 mg/kg MPTP 2 4 

MPTP 7 

 

 

O2 in 

situ 

MPTP 7 

 

TH 

MPTP O2 

 

O2 

MPTP 

 

P 

 

 

1 2 1 3 4 

1 

2 

3 4 

ASIC2a 

 

ASIC2a degenerin 

 

degenerin 2 

gain 

offunction 

ASIC2a 2 

430 

ASIC2a gainoffunction 

ASIC2a degenerin 

 

ASIC2aG430F ASIC2a 

TG 

TG 

TG

117 171 

P 

PACAP 

 

1,2 1,3 Randeep Rakwal 1 1 1 

1 1 1 1 1 1 

1 2 1 

1 

2 3 

 

PACAP 

PACAP 

PMCAO 

PACAP 

PACAP 

PMCAO PACAP38 

6, 24 RNA DNA 

PACAP 

1.7 

6 34 

24 Hedgehog 15 0.5 

6 

1524 

87 

PACAP 

PACAP 

 

P 

Protective effects of hyaluronan tetrasaccharide on hippocampal 

pyramidal neurons in neonatal mouse after hypoxicischemic injury 

Takehiko Sunabori, Masato Koike, Yasuo Uchiyama 

 

Hyaluronan HA is one of the major components of the extracellular matrix 

that contributes to a wide range of biological functions. The effects of HA differ 

mainly depending on the size of the polymers. Here we examined the effect 

of HA on hypoxicischemic HI brain injury against neonatal mice with a 

lowmolecularsize, HA tetrasaccharide HA4, and largemolecularsize HA 

800 kDa. Interestingly, only the HA4treated mice rescued the hippocampal 

pyramidal neurons 24hours after HI injury. To explore the underlining pathway, 

we focused on the contribution of the Tolllike receptor TLR /NFkB pathway, 

since the TLR2 and 4 are also known as receptors for HA. The TLR2 and TLR4 

null mice showed protective effects against HI injury, respectively. Moreover, the 

transient upregulation of the phosphorylation of p65/RelA, 1hour after HI injury 

was canceled when HA4 was administrated. Finally, the expression of the early 

inflammatory cytokine, IL1b was also inhibited by HA4 administration. These 

results suggest that the protective effect of neurons by HA4 is closely related to 

inactivation of the Tolllike receptor/NFkB pathway and reduced expression of 

inflammatory cytokines. 

P 

 

1 1 1 1 2 

3 4 1 

1 

2 

3 4 

 

4 

 

 

 

 

1 

 

 

5 mg/kg 

CA1 CA3, CA4 

 

mRNA 

 

Iba1 5 mg/kg 

 

 

 

 

P 

The Effects of an mer Peptide of Prosaposin in the Attenuation of 

MPP+/MPTP Toxicity in Vitro and in Vivo 

1 1 1 1 1 2 

1 

1 

2 

Parkinson’s disease PD is a chronic, progressive neurological disorder with 

increasing incidence in the aging population. In the present study, we used MPTP 

or 1methyl4phenylpyridnium ion MPP+induced dopaminergic neurotoxicity 

in C57BL/6J mice or SHSY5Y cells and explore the protective effect and 

mechanisms of an 18mer peptide of Prosaposin PS18 on dopaminergic 

neurons. PS18 2.0 mg/kg significantly improved behavioral deficits, and 

enhanced the survival of THpositive neurons and decreased the activity of 

astrocytes in the substantia nigra and striatum in MPTPinduced PD model 

mice. In vitro, the CCK8 assay and Hoechst 33258 staining clarified that PS 

18 300 ng/ml cotreated with 5mM MPP+ could protect the MMP+induced 

nuclear morphological changes and attenuated the cell death induced by MPP+. 

In addition, PS18 showed protection from MPP+/MPTPinduced apoptosis in the 

SHSY5Y cells and dopaminergic neurons in the PD model mouse via suppression 

of JNK/cJun pathway and up regulation of Bcl2 protein, down regulation of 

Bax, and inhibition of caspase3. 

P 

GABAA JM 

 

1 2 2 Raj Ladher 3 1 

1 

2 3 CDB 

 

JM1232 GABAA 

JM1232 

oxygenglucose deprivationOGD 

GABAA 

GABAA 

JM1232 

microarray system OGD 

OGD 

JM1232 

JM1232 

 

Real time RTPCR 

JM1232 

7 6 

GABAA 

 

 

 

P 

 

1 1 2 1 2 1 

1 

2 

 

 

 

Ed 

 

Ed 

, 

3.0 mg/kg 24 

1.22.4 mm , 

 

Nitrotyrosine NT 

24 

 

, Ed NT 

,

172 

117 

P 

ABC 

 

 

 

 

 

CSPG 

 

CSPG 

ABC 

 

 

ABC 

CSPG 

BDA 

 

 

CSPG 

ABC 

P 

 

 

 

 

 

 

8 SD 13 10 3 

C7C8 

234w 3 Little 

 

C1Th3Th4Th7Th8Th13L1 4 

5hydroxytryptamin5HT 

 

0.5 26 6 

14 5HT 2w3w4w 

5HT 

5HT 5HT 

 

 

P 

PACAP 

1 1 1 1 1 1 

1 1 2 1 

1 

2 

 

PACAP 

 

PACAP 

PACAP 

WT PACAP 

PACAP/ PACAP 

 

Benchmark 

stereotaxic impactor 0.5 mm Basso Mouse 

ScaleBMS WT 

PACAP/ 

3 

7 PACAP/ 

3 ssDNA PACAP 

/ ssDNA 

BMS 

PACAP 

 

P 

PMES 

 

Stefan Trifonov 

 

PMES2 

PMES2 

NeuN, GFAP, NG2, S100b, 

Iba1 single cell PCR 

 

PMES2 

115 

 

 

PMES2 

 

GFAP 

in situ hybridization PCR 

PMES2 

PMES2 

P 

Müller 

1 2 1 

1 

2 / 

 

 

Müller 

Müller 

 

Müller 

MAPK 

PI3K/AKTJAK/STAT5 

methylnitrosourea MNU 

Müller Erk1/2p38AKTStat3 

MNU 

12 TUNEL 3 4 

Müller Ki67MCM6BrdU 

MNU Müller Erk1/2p38AKT 

Stat3 3 

4 

Müller 

 

P

117 173 

P 

 

 

 

 

 

Vc 

Vc 

MAPK 

Wistar 7 

10 μl 0.9% 

pH7.25% pH4.05 1 

24 4% 

24 

30 μmanti 

Iba1antipERK1/2antip38 

Vc 

1 24 

Vc 

1 24 Vc 

 

 

 

P 

 

 

 

 

Macrophages infiltrate in the peripheral nerve and dorsal root ganglia DRGs 

after the peripheral nerve injury. Although macrophages are mainly divided into 

M1 classically activated and M2 alternatively activated subtypes, it is unclear 

what subtypes of macrophages infiltrate in the injured nerve and DRGs after the 

peripheral nerve injury. Many macrophages infiltrated around the injured region 

of the sciatic nerve on day 1 after the nerve injury. All macrophages infiltrated in 

the injured nerve were inducible nitric oxide synthetase iNOS + /arginase1 Arg 

1 M1 type. In contrast to the injured nerve, significant increase in macrophages 

was observed in the ipsilateral side of DRGs on day 2 after the nerve injury and 

almost all macrophages in the DRGs were iNOS /Arg1 + M2 type. Double 

immunofluorescence staining revealed that the increased macrophages were 

positive for CD163 and CD206. However, CD86 + macrophages were not increased 

in the DRGs. These findings suggest that functional phenotypes of macrophages 

infiltrated in the DRGs are distinct from those infiltrated in the injured peripheral 

nerves. 

P 

 

 

1 2 

1 

2 

2 15 CAP, 50 mg/kg 

CAP 20 

γcarrageenan carr., 2%, 20 μl Carr Base 

24 Hargreaves NTS, von 

Frey 

2 CAP NTS 

Base 2.3 carr CAP carr 

NTS Base 3.2 

6.2von Frey CAP carr 

15 CAP carr Base 

4.2 NTS 3.0CAP 

Base 7.5 CAP 

carr NTS Base 4.9 

von Frey CAP carr Base 

2 CAP NTS carr 

CAP TRPV1 

15 CAP NTS carr TRPV1 

von Frey TRPV1 

carr 

P 

Involvement of acidic microenvironment on the cancerinduced bone 

pain 

Masako Nakanishi 1,2 , Kenji Hata 2 , Toshiyuki Yoneda 2 , Yoshinori Otsuki 1 

1 

Dept. Anatomy, Osaka Medical College, 2 Dept. Biochem, Osaka Univ. Grad. Sch. 

Dent 

Microenvironments of bone metastasis are acidic due to the presence of cancer 

cells, inflammatory cells and boneresorbing osteoclasts. Since acid is a widely 

recognized algesic substance, we examined the effects of acid on the expression of 

calcitonin gene related peptide CGRP, which has been proposed to play a critical 

role in pain transmission. The expression of CGRP and transient receptor potential 

vanilloid subtype 1 TRPV1, an acidsensing nociceptor, was overlapped in 

dorsal root ganglion DRG. In organ culture of DRG, acid increased CGRP 

mRNA expression and this increase was significantly reduced by TRPV1 

antagonist IRTX. In addition, acid increased phosphorylation of CREB, a crucial 

transcription factor in neural functions. This was inhibited by IRTX. Knockdown 

of CREB expression or blockade of CREB signaling diminished acidinduced 

CGRP mRNA expression. Our results suggest that acidic microenvironments 

created in bone metastases activate TRPV1 in the sensory neurons, which 

in turn leads to upregulation of CGRP mRNA expression via activation of 

CREB transcriptional activity. These series of events may be involved in the 

pathophysiology of bone pain. 

P 

ILβ in trigeminal nucleus caudalis contributes to extraterritorial 

allodynia/hyperalgesia following a trigeminal nerve injury 

Mineo Watanabe, Shinji Hiyama, Takashi Uchida 

Department of Oral Biology, Division of Molecular Medical Science, Hiroshima 

University Graduate School of Biomedical Sciences 

The whisker pad WP area, which is innervated by the second branch of the 

trigeminal nerve, shows allodynia/hyperalgesia AH following transection of 

the mental nerve MN: the third branch of the trigeminal nerve. Glia is known 

to facilitate perception of noxious input, raising a possibility that these non 

neuronal cells are involved in the spread of AH at noninjured skin territory. After 

the MN transection, AH developed on the ipsilateral WP area. In response to 

MN transection, IL1β was upregulated in astrocytes in the trigeminal nucleus 

caudalis Vc. AH at WP area induced by MN transection was attenuated by IL1 

receptor antagonist IL1ra. Fos immunoreactive Fos+ neurons were observed in 

the Vc after nonnoxious mechanical stimulation of the WP area in the rats with 

MN transection. Administration of IL1ra also attenuated the number of Fos+ 

neurons. Administration of a noncompetitive antagonist of NMDA receptors MK 

801 reversed AH. IL1 receptor type I was localized in Fos+ and phospho NR1 

immunoreactive neurons. IL1β in the Vc might play an important role in the 

development of extraterritorial AH after MN transection. 

P 

cFos 

1 1 1,2 1 1 1,2 

1 

2 

 

 

 

 

 

 

cFos SD 

2 

1 5 

13 4 

cFos cFos 

1 cFos 

 

5 13 

1 

cFos

174 

117 

P 

Minocycline 

 

1 1,2 1 1,2 1 1 

1 

2 

P 

ITAM KO 

1 1 2 1 1 1 

2 1 

1 

2 

 

 

 

 

 

SD 2 

 

Minocycline 100 μg /day PBS 10 μl /day 8 

1 8 Minocycline PBS 

von Frey 

test 2 1 

1 

OX42 

Minocycline PBS 

 

 

 

 

 

 

 

 

ITAM DAP12 CARD9 KO 

L4 1 

 

Iba1 GFAP 

KO 

Iba1 

KO 

Iba1 

GFAP 

KO GFAP 

DAP12 CARD9 ITAM 

 

 

 

 

P 

Zitter 

 

 

 

 

Zitter 

Zi 

Zi 

 

Iba1 Ca2+ 

ED1 

3 

1Iba1 

ED1 

2 

ED1 3 

1 2 Iba1 

ED1 1 3 

2 

 

qPCR 

 

P 

 

1 1 2 1,3 1 

1 

2 

3 

 

 

 

 

DRG 

ERα 

GPR30 

 

OVX 

OVX+E 4 

 

OVX+E 

OVX 

 

 

DRG 

CGRP 

OVX+E OVX CGRP 

 

P 

Scaffold attachment factor B SAFB and SAFB synergistically 

inhibit intranuclear mobility and function of ERα 

 

 

Estrogen receptor alpha ERα plays a key role in physiological processes as a 

transcriptional factor that is regulated by cofactors. ERαmediated transcriptional 

regulation is correlated with the mobility of ERα in the nucleus associating with 

the nuclear matrix, the framework for nuclear events including transcription. 

However, the relationship between ERα mobility and the cofactors is unclear. 

Scaffold attachment factor B1 SAFB1 and its paralog SAFB2 are nuclear matrix 

binding proteins that have been characterized as ERα corepressors. Here, using 

chimeric fluorescent proteins, we show that SAFB1 and SAFB2 colocalize and 

interact with ERα in the nucleus of living cells after 17βestradiol E2 treatment. 

Fluorescence recovery after photobleaching analysis revealed that SAFB1 and 

SAFB2 each decrease ERα mobility, and coexpression of SAFB1 and SAFB2 

causes a synergistic reduction in ERα dynamics under E2 treatment. In accordance 

with these changes, ERαmediated transcription and proliferation is cooperatively 

inhibited by SAFB1 and SAFB2. These results indicate that SAFB1 and SAFB2 

are crucial repressors for ERα dynamics and function in association with the 

nuclear matrix. 

P 

Estrogen αfetoprotein 

 

 

Estrogen Aromatase 

Estrogen 20 

Estrogen 

Estrogen 20 

Estrogen 

αfetoprotein AFP Estrogen 

AFP Estrogen 

5-40 

Wistar AFP Western 

blotting Realtime PCR 

Estradiol17β EIA kit 1 AFP 

5 20 2 

AFP 20 3 AFP 

20 4 

Aromatase AFP 5 AFP 

Estrogen AFP 

Aromatase 

AFP Estrogen

117 175 

P 

 

 

 

C 

 

β ISL1 

NKX2.2 NKX2.2 NKX6.1 C 

 

β PDX1 

12 

18 1 

RTPCR β 

MafA Ngn3 

PCR 14 1 

MafA 

1 14 15 

MafA 14 65 

PDX1 10 β 

PDX1 

β PDX1 

 

 

P 

 

 

 

 

 

 

 

RFamide 

 

 

 

C GlyLeuTrpNH2 

GLWamide ArgPheNH2 RFamide 

Hym176 APFIFPGPKVamide 

CysGLWamide, CysRFamide, Cys 

Hym176 

ELISA 

 

1 3 1 

 

 

P 

 

 

1,2 2 2 1 

1 

2 


 

NEDA 

GPR54 

GnRH 

NEDA fos 

NEDA 

NEDA 

tyrosine hydroxylase TH 

TH 

 

NEDA TH 

TH 

 

TH 

NEDA TH 

 

 

P 

manserin 

 

 

 

manserin 

Neuroreport 15: 

17551759, 2004; Histochem Cell Biol. 134: 5357, 2010; Int J Pept Res Ther. 17: 

193199, 2011 manserin SgII 

 

manserin 

manserin 

manserin 

manserin 

manserin TSH 

manserin TSH 

 

manserin SgII 

manserin 1/3 

manserin 

 

manserin 

 

P 

βreductase 

 

1 

 

 

 

 

3αhydroxysteroid dehydrogenase 3αHSD 3βHSD 

 

 

5βreductase 

 

5βreductase COS1 

 

 

 

 

P 

 

 

 

V 

 

 

 

A, B1, B2, C 4 

A C 

B1 B2 

 

 

 

 

 

 

 

B1 B2 

C 

A

176 

117 

P 

PACAP 

 

1,2 1,3 1,2 1 1 

1 1 1 Jozsef Farkas 1 1 

1 1,2,4 2 1 

1 

2 3 

4 

 

PACAP NMDA 

RGC 

 

 

NMDA PACAP RGC 

MG/Mφ 

NMDA 

PACAP PACAP10 8 10 12 M NMDA 

 

Iba1 PACAP 10 10 M 

3 MG/Mφ NMDA 

MG/Mφ RGC RTPCR 

tgf-β 

1 PACAP 10 10 M 

TGFβ1 

MG/Mφ TGFβ1 

PACAP 

 

P 

 

1,2 Thomas Finger 2 3 3 3 

1 1 

1 

2 Rocky Mountain Taste and Smell Ctr, Uni. 

Colorado Med Sch 3 

ATP ATP 

P2X 

P2XP2Y ATP ATP 

NTPDase2 ATP NTPase2 

ATP 

 

4 

A1A2AA2BA3 

 

RTPCRin situ hybridization 

A2B 

 

P 

Mash AADC GAD 

1 1 2 1 

1 

2 

3 

 

bHLH Mash1 3 Mash1 

3 Mash1 

3 

Mash1 

 

Mash1 

 

AADC GABA 

GAD67 Mash1 

Mash1 3 AADC, GAD67 

 

P 

P 

 

1 2 3 2 1 

1 

2 

3 

 

substance P SP 

15 

2 4%PFA 

SP 

1/4 

3/4 

SP 

 

 

SP 

 

SP 

3/4 

1/4 

SP 

3/4 SP 

 

 

P 

AB 

 

 

 

 

3 

 

3 

 

3 

ATP 

P2X2 P2X3 

P2X2 P2X3 

ATP 

 

 

 

Double in situ hybridization 

3A B 

 

P 

 

1 2 1 

1 

2 

 

 

Ca 2+ [Ca 2+ ] i Ca 2+ 

imaging HPLC 

 

HPLC 

150 μm 

Ca 2+ indicatorfura2 

HEPESRinger buffer HPLC 1:10000[Ca 2+ ] i 

 

HPLC UV 

 

[Ca 2+ ] i

117 177 

P 

 

1 2 3 4,5 4 1 

1 

2 3 

4 5 

 

 

 

 

 

HE 

 

 

 

PAS 

PAS 

GolfNCAMvimentin TAAR2 

 

G 

TAAR2 

 

 

P 

 

1,2 2 1 2 

1 

2 

 

 

 

Wistar 

 

2KOHcollagenase 

Ushiki & Ide, 1988 

 

10% 

 

KOHcollagenase 

 

1 

 

 

 

 

 

P 

 

 

 

 

ACh 

 

ACh mAChR 

NRK52E 

CarbacolCch 60 10 12 

3 

mRNA RTPCR mAChR 

NRK52E 

mAChR M1R M4R Cch 

10 M2R 3 M4R 

M2R M2R M4R 

 

ATP K + Kir6.1 30 M4R 

Kir6.1 M4R 

M4R Kir6.1 

NRK52E in vivo 

 

P 

 

 

 

 

 

IR 

LC3 

LC3I LC3II LC3 

 

IR IR 4 24 

LC3 

LC3 24 

LC3 

LC3 

IR LC3 

D,L IR 

 

 

 

IR 

 

P 

LPS 

 

1 2 1 1 3 

1 

1 

2 

3 

 

 

 

 

LPS 

 

12 9 3 1 

2 LPS 3 LPS 

2 LPSErsherichia coli O157 5 mg/kg 

0 3 10 

3 LPS 0 3 10 3 

17 1 0 3 

HE PAS 

LPS 

 

LPS 

 

 

P 

 

 

G1 

MCTs 

MCT 

 

CD147EMMPRIN 

MCT1MCT2MCT4 

 

MCT1 

MCT2 

MCT4 Leydig 

MCT2 

 

MCT2 

 

MCT1 MCT4 MCT2 

MCT2 

MCT1MCT2 

MCT4

178 

117 

P 

Separatration of early stage acrosome reacted sperm and analyses 

of the proteins 

Kenji Yamatoya, Chizuru Ito, Cheng Chen, Mamiko Maekawa, 

Yoshiro Toyama, Kiyotaka Toshimori 

Dept. Anatomy and Developmental Biology, Grad. Sch. Med., Univ. Chiba 

During the acrosome reaction, the contents of sperm acrosome are released from 

the original location to the final destination and working place. The molecular 

distribution and nature are posttranslationally modified. We reported that flow 

cytometric analyses using antiacrosomal membrane protein antibodies anti 

IZUMO1 and antiSPACA1 can discriminate the early stage of acrosome reaction, 

classifying into 7 populations. 

In this study, we analyzed the 7 populations and found that the populations 

number 4 and 5 contain acrosomeswelling sperm. Acrosomeswelling occurs at 

a very early stage of acrosome reaction which starts before the exposure of inner 

acrosomal membrane. One of the acrosomal membrane proteins, SPACA1, was 

cleaved during this swelling stage. Western blot showed that SPACA1 was 35 kDa 

before acrosome reaction and became 21 kDa in acrosomeswelling sperm. 

Thus, this flow cytometric method is useful to analyze the detailed acrosome 

reaction process and the molecular changes during the acrosome reaction which 

are the last preparatory process for the spermegg interaction. 

P 

Cell adhesion molecule Nectin 

 

Kannika Adthapanyawanich 

 

 

Cell adhesion molecule1 Cadm1 Nectin3 

Cadm1 

Poliovirus receptor 

Nectin3 Nectin2 

 

Cadm1 Nectin3 


 

Cadm1//Nectin3/ Cadm1/ 

Nectin3/ 

 

 


 

P 

SF 

 

 

 

SF1 

 

 

2 anti 

Müllerian hormone type 2 receptor Cre CreloxP 

SF1 

0 21 SF1 

7 

14 21 

 

14 21 SF1 

AMH 

p27 

WT1SOX9GATA4androgen receptor 

SF1 

SF1 

 

P 

Autoimmune responses induced by immunization with xenogenic 

testicular germ cells alone 

 

 

Experimental autoimmune orchitis EAO is one of the models of immunological 

male infertility. Classically, the immunization of mice with a testicular 

homogenate emulsified in complete Freund's adjuvant followed by intravenous 

injections of Bordetella pertussis is necessary for the induction of murine EAO. 

Later, we previously established a mouse EAO model that can be induced by two 

subcutaneous injections of viable syngeneic testicular germ cells TGC alone, 

and the autoantigens involved in the mouse EAO have been analyzed using two 

dimensional gel systems and western blotting. In the present study, to examine 

whether the xenogenic TGC can induce a mouse EAO, we immunized mice with 

viable TGC taken from the rat or the guinea pig. The results showed that mouse 

EAO was also inducible by the rat TGC but not the guinea pig TGC. Therefore, 

the results suggest that the autoantigens that induce the mouse EAO are present in 

not only the mouse TGC but also xenogenic TGC = the rat TGC. Analyses of the 

common autoantigens responsible for EAO induction are now in progress. 

P 

Diethylhexyl phthalate 

 

 

Di2ethylhexyl phthalate=DEHP 

 

 

DEHP 

A/J 

8 0%0.01%0.1%DEHP 8 

 

0.01%DEHP 0.1%DEHP 

 

0.01% 

0.1%DEHP 

DEHP 

MHC Class RTPCR 

DEHP 

IL10IFNγ 

DEHP 

 

P 

The effect of cadmium on immunoenvironment in the testis 

Yuki Ogawa 1,2 , Masahiro Itoh 1 , Shuichi Hirai 1 , Munekazu Naito 1 , 

Ning Qu 1 , Hayato Terayama 1 , Hidenobu Miyaso 2 , Yoshiharu Matsuno 2 , 

Masatoshi Komiyama 2 , Chisato Mori 2 

1 

Dept. Anat., Tokyo Med. Univ., 2 Dept. Bioenv. Med., Grad. Sch. Med., Chiba Univ. 

Cadmium, one of various environmental toxicants, is known to suppress systemic 

immunity and to injure the testicular capillary endothelia with resultant necrosis of 

testicular tissues in mice and rats treated with high doses. Recently, it also became 

evident that cadmium can affect the integrity of the bloodtestis barrier BTB, 

the endocrine function of Leydig cells, apoptosis of germ cells and systemic 

immunity, even on treatment with a low dose that does not induce spermatogenic 

disturbance. Experimental autoimmune orchitis EAO, i.e., an organ 

specific autoimmunity of the testis, can be induced by repeated immunization 

with testicular antigens, and its pathology is characterized by lymphocytic 

inflammation and spermatogenic disturbance. In the present study, we investigated 

the morphological and functional changes of testes in mice treated with a low dose 

of cadmium chloride CdCl 2 and also examined its toxicity as to susceptibility to 

EAO.

117 179 

P 

TIRF 

 

 

 

ECM 

 

ECM 

 

1 I 

Col1FN2 

VI Col6fib3 IV 

Col4TIRF 

ECM 

ECM 

ECM 

Col1FN 

fib Col4 

Col1FN Col6 fib Col4 Col6 

 

Col6 

 

P 

PACAP TAC 

1 1 2 3 4 

1 

2 

3 4 

 

2 

 

 

 

PACAP 

PACAP PAC1R 

PACAP 

 

PACAP 

 

PACAP 2 

PACAP 

PACAP 

PACAP 

DNA 

3 40 

P 

A TAC1 6 

 

P 

in vitro 

1 2 2 3 

3 2 2 3 1 

1 

2 3 

 

 

10 

 

 

 

3 

 

3 

in vitro 

 

1 mm 

endometrial 

glands 

 

 

in vitro 

 

 

 

P 

Localization of fatty acid binding protein in mouse placenta and its 

possible role of fatty acid transport through trophoblasts 

Ariful Islam, Nobuko Tokuda, Yasuhiro Adachi, Tomoo Sawada, Yuji Owada 

Department of Organ Anatomy, Yamaguchi University Graduate School of 

Medicine 

Background: Malnutrition of mother during pregnancy is now thought to 

contribute to the etiology of various metabolic and/or neural disorders that 

manifest throughout life. Placenta is the key organ for regulating the transfer 

of nutrients including fatty acids from mother to embryo. In this study, we 

investigated the functional role of fatty acid binding protein 3 FABP3 after 

determining the localization of FABPs, cellular fatty acid chaperons, in the mouse 

placenta. 

Results: In RTPCR, gene expression of FABP3, 4 & 5 was detected. The protein 

expression of FABP 3, 4 & 5 was also confirmed by western blot analysis. In 

immunohistochemistry, FABP3 was highly expressed in the labyrinthine transport 

zone of mouse placenta; FABP4 was highly expressed in deciduas basalis zone; 

FABP5 was weakly and widely distributed in the labyrinthine, decidua and 

spongiotrophoblast zone. 

Discussion: FABPs were expressed in the mouse placenta with spatial 

differences. FABP3 and 5 were suggested to be involved in regulation of cellular 

transfer of fatty acids in trophoblasts of placenta. The functional significance of 

FABP expression in trophoblasts is now under investigation. 

P 

Kardasewitsch Verocay 

 

1,2 2,3 2,3 4 4 

1 

1 

2 3 

4 

 

 

 

 

 

Kardasewitsch Verocay Kardasewitsch 

Verocay 2 HE 

 

 

Verocay Kardasewitsch 

 

 

 

 

P 

MAP 

1 2 

1 

2 

MAP 

 

MAP in situ 

 

MAP 

 

 

 

 

13 17 

ERK1/2 

 

JNK1/2 

ERK1/2 p38 ERK5 

 

MAP 

ERK1/2 JNK1/2

180 

117 

P 

 

 

 

 

 

 

QD 

 

193 2% 

HE 

QD AF 

Alb 

QD 1 

QD 2 

5 QD 

AF 

 

Alb 

 

QD 

 

P 

In vivo labeling of halogenated volatile anesthetics via intrinsic 

molecular vibrations using nonlinear Raman spectroscopy 

Masahiko Kawagishi 1 , Takayuki Suzuki 2 , Yu Nagashima 1,2 , Sumio Terada 1 , 

Kazuhiko Misawa 2 

1 

Sect. Neuroanat. Cellular Neurobiol., Dept. Systems Neurosci., Tokyo Med. 

Dent. Univ., 2 Dept. Applied Physics, Tokyo Univ. Agriculture and Technology 

Halogenated volatile anesthetics are frequently used for inhaled anesthesia 

in clinical practice. No appropriate biological method has been available for 

visualizing their localization in action. Therefore, despite their frequent use, the 

mechanism of action of these drugs has not been fully investigated. We measured 

coherent antiStokes Raman scattering CARS spectra of sevoflurane and 

isoflurane, two of the most representative volatile anesthetics, and determined the 

lowfrequency vibrational modes without nonresonant background disturbance. 

Molecular dynamics calculations predict that these modes are associated with 

multiple halogen atoms. Because halogen atoms rarely appear in biological 

compounds, the entire spectral landscape of these modes is expected to be a 

good marker for investigating the spatial localization of these drugs within the 

intracellular environment. Using live squid giant axons, we could detect the 

unique CARS spectra of sevoflurane for the first time in a biological setting. 

P 

Fluorescence Immunohistochemistry by Confocal LSM for Studies of 

SemiUltrathin Specimens of Epoxy ResinEmbedded Samples 

1 2 

1 

2 

 

We have developed a technique, using a combination of immunofluorescence 

staining of semiultrathin sections of epoxy resinembedded samples and the DIC 

images and images in transmission mode obtained by LSM, that provides detailed 

information about the immunolocalization of antigens and histological and cellular 

structures. To demonstrate the effectiveness of our method, we examined the 

immunofluorescence of immunostained K13 and K14 and that of immunostained 

CII and CIII and the corresponding DIC and transmission images during the 

morphogenesis of filiform papillae on the lingual epithelium of rat fetuses and 

juveniles. We demonstrated that our newly developed technique for localization of 

pairs of antigens should be useful for investigations of very small specimens, such 

as fetal tissues and organs. 

P 

/ 

 

1 2 2 3 1 1 

2 1 2 

1 

2 

3 

Quantitative analysis of morphological synaptic connectivity depends on exact 

threedimensional reconstructions of synaptic ultrastructure using electron 

microscopy of serial ultrathin sections. However, reconstructions from serial 

section transmission electron microscope TEM are extremely timeconsuming 

and difficult. To overcome these problems, we have applied a new three 

dimensional reconstruction method that involves the combination of focused ion 

beam milling and scanning electron microscopy FIB/SEM. Dendrites of the rat 

neostriatum neurons were visualized by a recombinant virus vector, which labeled 

the infected neurons in a Golgi stainlike fashion. Axon terminals and dendritic 

spines in the rat neostriatum neurons were stained by the immunofluorescence 

method. The appostitions of dendrites to the inputs were detected three 

dimensionally with a confocal laserscanning microscope. Then, these sections 

were stained by peroxidase anti peroxidase method and observed three 

dimensionally with a focused ion beam milling and scanning electron microscopy 

FIB/SEM. 

P 

 

 

 

 

 

3 

 

 

Atomic Force Microscopy; AFM 

 

 

DNaseI 

AraC 5 4 

AFM Alexa488 phalloidin 

 

 

AFM P C 

 

 

 

 

 

P 

GP 

 

1 1 1 1 2 

3 4 

1 

2 

3 4 

 

 

 

 

 

 

 

 

GP 

 

 

 

30 

 

PC 

 

524

117 181 

P 

 

1 1 1 1 1 

2 1 

1 

2 

SGL 11 4 

23 4 SGL 

TBL SGL 

SGL 

SGL 

 

SGL 

 

 

 

 

 

 

 

 

 

 

SGL 

 

 

P 

iPad 

 

 

23 4 

iPad 30 

CT MR 

4 

1 1 

2 

3 

4 

DICOM Osirix CT MR 

2 3 

 

23 

Ai Autopsy imaging CT 

 

 

 

 

 

P 

eLearning 

 

 

eLearning 

eLearning 

eLearning 

 

Moodle Moodle 

 

 

P 

 

1,3 1 1 1 2 

2 2 2 2 2 

1 

2 3 

PT 

 

 

16 

 

5 

 

22 

 

PTOT 

 

 

 

 

PTOT 

 

 

PTOT 

 

P 

 

1,2 3 3 3 2 

4 1 1 1 1 1 

1 

2 3 

4 

 

 

 

 

 

 

 

 

P 

 

1 2 2 3 4 

1 

2 3 4 

 

 

 

 

45

182 

117 

P 

 

1,2 1 1 1 1 

1 1 1 1 1 

1 

2 

 

 

 

22 

46 

 

 

1 3 

 

 

 

 

 

 

P 

 

 

 

 

 

 

 

22 355 

 

 

 

 

 

 

 

99 

76 

99 

 

 

P 

 

 

1 2 3 3 4 5 

1 

2 3 

4 5 

 

 

 

66 

78 

2 

 

 

2005 

138 

 

 

77.5%107 

62.3%86 25.4%35 

14.5%20 

 

 

 

 

 

P 

 

 

 

 

 

 

4 1 

 

 

 

 

 

2 83 

 

 

 

10 

 

 

 

 

 

 

P 

MRI 

1 2 2 3 1 1 

4 4 5 6 

1 

2 3 

4 5 

6 

 

 

 

MRI 

95 59 36 23.27.8 

Hüter 

20 mm 40 mm 3 mm 

 

Hüter 

 

 

Hüter 

3.441.00 mm 4.041.18 mm 

3.450.88 mm BMI 

p

117 183 

P 

 

1 2 1 1 1 

1 

2 

4 

 

 

 

1 5 MP PIP 

DIP 

1 

 

 

 

 

 

 

 

 

P 

 

 

 

 

 

 

 

 

 

MRI 

 

 

Apple Osirix Realia 

 

 

 

 

 

 

 

 

 

P 

 

 

 

 

 

1 

 

 

 

 

VoxBlast 

KY500 

 

3 μm 

3 μm 

 

KY 

500 

3 

 

 

P 

Fossa 

 

 

Terminologia Anatomica 

Fossa 

Fossa 1Fossa 

2Fossa 3Fossa 



8Fossa 

Fossa olecraniOlecranon 

olecrani 

Fossa canina 

Musculus levator 

anguli oris Musculus caninus 

 

Fossa radialis Radius 

radialis 

 

 

 

P 

 

 

 

FA 

2008 

3 

2008 3 

FA 0.1 ppm FA 

2009 

FA 710 7 

 

FA 

40 4 2 50 

3 5 2 

FA 2 FP30 

00.4 ppm GASTEC 0.15 ppm 

FA 

FA 

 

P 

 

1,2 1 1 1 1 

1 

2 

 

20082009 

FA FA 2 

5 0.1 ppm 

FA 

FA 

20102011 

10% 3.5%FA100% 

3 

FA 4 

4/ FA 

10 cm 

30 FA 0.01 

ppm 2010 FA 

FA 

FA 2010 

DNPHHPLC 11 

0.0064 ppm 0.0039 ppm 1/10

184 

117 

P 

Thiel 

1 1 2 2 2 

3 2 1 

1 

2 2 3 

 

Thiel Graz Dr. Thiel 

 

 

 

Thiel 

 

 

 

16 

8 Thiel 4 

 

 

Thiel 

 

 

 

Thiel 

 

P 

 

 

1 1 2 2 1 

1 1 1 1 

1 

2 

2008 

FA 0.1 ppm 

FA 

FA 

 

 

20 

FA 

FA 

FA B 

0.1 ppm 

B FA 

 

FA 0.1 ppm 

FA 0.1 ppm 

FA 

 

 

P 

 

1 2,3 2,3,4 

1 

4 2 

3 4 

 

 

 

 

 

 

 

6 

 

Hedgehog 

 

Hedgehog SmoothenedSmo 

Smo 9 

cKO 

 

SmocKO 

 

 

SmocKO 

 

P 

 

1 1 2 2 2 

3 3 3 3 

1 

4 2 3 

3 

 

 

 

PF 

PF Cholera toxin b subunitCTb 

CTb cFos 

mapping CTb 

 

CA1 

C1 

 

CTb cFos 

 

 

 

 

 

P 

Tubulin polyglutamylation regulates cytoskeletal distribution in brain 

Kenji Ohata 1 , Yoshiyuki Konishi 2 , Reiko Tsuchiya 3 , Koji Ikegami 1 , 

Mitsutoshi Setou 1 

1 

Dept. of Cell Biology and Anatomy, Hamamatsu Univ. Sch. of Med., 2 Dept. of 

Human and artificial intelligent Systems, Univ. of Fukui, 3 MitsubishiKagaku 

Institute of Life Sciences 

Microtubules form the structural basis of neuronal morphology and partake in 

directing intracellular transport. Tubulins, components of microtubules, are known 

to be highly polyglutamylated in neurons, and we, along with another group, 

have recently shown that a part of tubulin tyrosine ligaselike Ttll proteins 

catalyzes polyglutamylation of tubulins though the subsequent function of this 

polyglutamylation remains unclear. Present study demonstrates that Ttll1 and Ttll7 

play major roles in adding glutamate chain to alpha and beta tubulin respectively 

in the brain. Ttll1 or Ttll7 singleknockout mice exhibited only a slight decrease 

in total polyglutamylation. To reveal the neuronal polyglutamylation function, we 

generated Ttll1Ttll7 doubleknockout mice which showed significant decrease 

in neuronal polyglutamylation. Immunohistochemical and electron microscopic 

analyses showed fewer neurofilaments in dendrites of cortical neurons in the 

doubleknockout mice compared to the wild type, while the neurofilament 

distribution in Ttll1 or Ttll7 singleknockout mice was similar to that of the 

wild type. These results suggest that polyglutamilation regulates neurofilament 

arrangement. 

P 

KCC 

1 2 

1 

2 

3 KCC2 Cl – 

GABA 

 

KCC2 in situ hybridization 

in situ hybridization KCC2 

mRNA 5 P5 P7 

P14 

 

P7 

VGluT2 

P14 KCC2 

P7 

KCC2 

 

2 KCC2 mRNA 

GABA 

KCC2

117 185 

P 

 

 

 

 

 

NMJ 

 

 

 

NMJ 

 

d 

NMJ 

 

 

NMJ 

P 

 

 

 

Hh 

 

 

 

Hh 

 

Hh 

SC 

Hh 

SC 

 

Hh 

SC 

 

 

Hh 

 

P 

neuroligin 

 

 

 

 

3 

neuroligin 

neurexin 

Neuroligin 

 

Neuroligin family 

neuroligin 

 

neuroligin 1EGFP dsRed2 

neuroligin1 

Neuroligin1 

neuroligin1 

neuroligin 

 

P 

 

1 2 2 2 3 

2 

1 

4 2 3 

 

 

 

 

 

 

 

 

 

 

 

 

ABCD 

 

 

 

 

ABCD 

 

 

P 

 

1 1 1 1 2 2 

2 2 

1 

3 2 

 

4 3 

 

 

 

22 74 

 

4 4 

2 

 

 

 

1992IX1 0.8% 

 

P 

 

1 1 1,2 1 2 

2 2 

1 

2 

 

Gi F O 

Tr 3 Nf 

Gi 2 L5/S1 S1/S2 S1/S2 

S2/S2 S1/S1 S2/S2 Gi S1/S2 

S2/S2 S2/S3 

1Gi2 4 L5/S1 S1/S2 1 Nf L4L4 

3 Tr>F>O L4 Tr 

S1/S2 S2/S2 2 Nf 

L43 F>Tr>O S1/S1 

S2/S2 1 Nf L4F>O>Tr 

 

2Gi1 5 S1/S2 1 L3 Tr Nf L3+L4 

S2/S2 2 Nf L4F>Tr>O 

1 F>O>Tr 

1 S2/S3 2 L4 L5 F, O, Tr 3 Nf 

L4+L5 

Gi

186 

117 

P 

 

1 1,2 1 2 2 

2 

1 

2 

 

Gs F O 

Tr 3 Nf 

 

Gs L4/L5 L5/S1 S1/S2 

1L4/L5 5 Nf L3+L41 L44 

Nf L3+L4 Nf L4 L4 3 

Tr>F>O3 L4 Tr 

L4 3 F>Tr>O 1 

 

2L5/S1 5 Nf L42 L4+L52 L51 

L4 F>Tr>O 1 

F>O>Tr 1 

L4+L5 L4 3 F>O>Tr L5 FTr 1 

L5 FOTr 1 Nf L5 L4 Tr 

L5 3 

 

3S1/S2 1 Nf L4+L5 

Gs 

 

P 

 

 

 

 

 

 

 

3 

70 90 4 

 

50 

PGP9.5 

P 

CCD 

 

 

P 

 

 

P 

 

 

P 

 

1 2 2 2 

1 

2 

22 92 1 

 

 

 

 

 

 

 

 

 

Humphry 

Miyauchi 

Humphry 

 

 

 

P 

 

1 1,2 1 2 2 

2 

1 

2 

 

H23 A. mediana, M 

A. brachialis superficialis, BS BS 

Ax 

Ax BS 

A. radialis, 

R A. ulnaris, U BS 

A. antebrachii superficialis, AS AS 

 

M M 

U R 

R 

R M U 

M BS 

 

BS 

M 

 

P 

 

1 1 1 1 2 3 

3 

1 

2 3 

 

 

 

 

 

 

 

75 

 

 

 

 

 

1 

 

 

 

 

P 

 

1 2 3 4 4 

4,5 2 

1 

2 

3 

/ 4 

5 

5 DP 

DP 7 #1-7 DP 

#1 #2 #3 #1 

#2 

#3 DP 

DP 

#4 #5 #4 #5 

#4 #5 

DP #6 #7 #6 

 

#7 

7 

 

#1367 

#245 

cranial ventral dorsal 

caudal ventral

117 187 

P 

 

 

 

 

 

2023 66 Ruge 

5 I 

2 3II 

1 10 15III 8 

12IV 

40 61V 6 9 

 

 

4 

6 

9 

2 

 

III 

IV 

 

 

P 

 

 

 

 

2011 

2 

 

 

 

 

• 

 

 

 

• • 

 

1952 2011 2161 

2 – 

 

2011 2 

3 

 

P 

 

1 1 1 1 1 

2,3 3 3 

1 

2 2 

3 

2011 

1 81 

 

5 

 

2 

2 

1 1 3 

 

2 2 

 

 

 

3 89 

 

5 

 

 

P 

 

1 1 2,3 2 2 

1 

2 3 

 

 

 

 

36 

 

14 6 5 

1 

2 2 

3 

2 

4 2 

 

 

5 

3 

 

 

 

 

 

 

P 

 

1 1 1 1 1 

1 1 1 1 1 1 

1 1 1 1 1 2 

1 

3 2 

 

 

 

27 

CTCBCT 

 

 

80 1 

 

 

 

 

 

 

P 

 

1 1 1 1 2 3 

4 2 

1 

2 3 

4 

2011 77 

 

 

 

5 

 

1/3 

 

1 

 

 

3.5 mm 

9 cm 

 

1 

1 

2

188 

117 

P 

 

1 1 1 1 1 

2 2 2 2 

1 

3 2 

 

 

0.15-0.48 22 

70 

12.5 cm 

12.5 cm 

 

328 g 

6 

 

1 

 

 

 

P 

 

1 1 2 2 

1 

3 2 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

P 

KUHWrat 

 

 

KUHWrat 

1720 100% 

90 Wtype Stype 

Ntype 

 

742 Stype 178 13475.3 

4424.7 

Stype 

 

3823.4 1840.9 

2 

96 53.9 26 14.6 

Ntype 38 

8 21.1 3 7.9 11 39.3 

 

Ganzer, Maynert, Gudden 

 

Wtype 

R=0.875 

P 

 

 

1 

2 

3 4 

5 

9 

 

 

 

Z 

 

AI 

AI Z 

 

I 2 3 Z 

 

 

Z 

I 1.5 4 

9 

Z 

Z I Z 

I 

 

P 

 

 

 

 

 

P 

 

 

 

3 4 

 

50 

 

PGP9.5 P CCD 

 

 

18 P 

 

 

3 

 

 

P 

P 

 

 

 

 

 

 

 

 

ICR 18 

 

6 5 FITC 

4%PFA 

 

3 

3 

5 

HIF1α

117 189 

P 

 

1 1 1 1 1 

1,2 1 1 1 1 

1 

2 

 

2AQP2 

VP 

VP 

VP 

VP AQP2 AQP2 

AQP2 

4 

256 256S 269 269S 

AQP2 

VP DDI/Ddia 

VP 

256S 

VP 256S 

256S VP 

VP 269S AQP2 

 

AQP2 

269S 

P 

myosinIIA 

 

 

 

 

Epstein 

myosinIIA 

PLP 1 

10%15% 20 sucrose OCT 

 

 

 

 

myosinIIA 

 

myosinIIA 

 

PAN myosinIIA 

myosinIIA 

myosinIIA 

 

P 

FABP 

1 1 1 1,2 1 1 

1 

1 

2 

 

 

 

 

 

FABP5 

FABP5 blimp1 

cmyc 

FABP5KO CRABP2 

blimp1 cmyc 

FABP5KO 

30 

FABP5KO 

FABP5

190 

117 

 

ATP 

 

 

ATP 

ATP P2X 

ATP G P2Y 

TRPV1 

DRG P2XP2Y mRNA 

in situ hybridization ATP 

ATP 

ATP P2Y12 

p38 MAPK 

 

ATP

117

117 191 

 

 

 

Adachi, Yasuhiro 

2P019 

Fujiwara, Mutsunori 

3OFPMVI2 

 

2P093 

3P073 

Fujiwara, Naoki 

1P127 

Jindatip, Depicha 

3ODPMI4 

Adthapanyawanich, Kannika 

2P098 

Grienberger, Christine 

S264 

Josef Flor, Peter 

1P031 

3P065 

Hagiwara, Haruo 

2P125 

Jue, Seong Suk 

1P128 

Agur, Anne 

3OHPMIV5 

Hampel, Falko 

3OGPMIV2 

Kagawa, Yshiteru 

2P019 

Aldartsogt, Dolgorsuren 

3OHPMI1 

 

1P096 

Kakizuka, Akira 

3OGPMIV3 

1P070 

Harada, Hidemitsu 

1P127 

Kameie, Toshio 

2P061 

Ali, Md Moksed 

1P062 

Hart, Gerald W 

1OIPM1 

Kamiya, Mako 

2P023 

 

2P093 

Hashino, Eri 

2OGAMI1 

Karasawa, Nobuyuki 

1P019 

Aoyama, Fumiyo 

S211 

Hashizume, Wataru 

1P073 

Karim, Mohammad Rabiul 

2P001 

Arakawa, Takamitsu 

3OHPMIV5 

Hata, Kenji 

3P037 

2P010 

Asano, Yoshiya 

1P065 

Hayashi, Ayako 

2P023 

Kawagishi, Masahiko 

3P077 

Asanuma, Daisuke 

2P023 

Hayato, Terayama 

2P129 

Kawano, Hitoshi 

2P032 

Astrid Feinisa, Khairani 

2P056 

2P131 

Kawata, Mitsuhiro 

1P031 

2P062 

Hebiguchi, Taku 

2P108 

Kikkawa, Masahide 

2P049 

2P074 

Hill, Daniel 

S264 

2P050 

2P075 

Hirai, Shuichi 

3P069 

Kikuchi, Kunio 

1P062 

Atoji, Yasuro 

2P001 

Hiraoka, Mari 

3OIPM4 

Kim, Byung In 

1P128 

Atukorala, ADSL 

3OGPMVI1 

Hiyama, Shinji 

3P038 

Kim, Ji Youn 

1P128 

Baba, Otto 

3OGPMVI1 

HoriiHayashi, Noriko 

1P002 

kishigami, Ryota 

1P127 

Bastmeyer, Martin 

3OGPMIV2 

1P037 

Kitamura, Seiichiro 

3OHPMI1 

Blomhoff, Rune 

3OFPMVI2 

Huang, Zheng 

2P025 

Kiyama, Hiroshi 

S13 

Bonner, William 

1OGPMII3 

IdaYonemochi, Hiroko 

2OGAMII1 

Klein, Ruediger 

3OGPMIV2 

Buzsaki, Gyorgy 

S263 

Ide, Soyuki 

S211 

Kogaya, Yasutoku 

1P019 

Chen, Cheng 

3P064 

Ikegami, Koji 

3P104 

Koike, Masato 

3P023 

Chen, Huayue 

1P019 

Imai, Katsuyuki 

3OFPMVI2 

Koji, Takehiko 

S224 

Chen, Jiaorong 

1P004 

2P108 

Kokubu, Keiji 

2P005 

2P025 

Inaga, Sumire 

2P052 

3P013 

 

S43 

2P061 

Komiyama, Masatoshi 

3P069 

3OFPMVIII1 

Inohara, Keiji 

3OGPMVI1 

Kondo, Takako 

2OGAMI1 

Dalkhsuren, ShineOd 

3OHPMI1 

Iseki, Shoichi 

2P097 

Konishi, Yoshiyuki 

3P104 

1P070 

2P098 

Konnerth, Arthur 

S264 

del Toro, Daniel 

3OGPMIV2 

Ishibashi, Osamu 

1P062 

Korekane, Hiroaki 

1P037 

Dobberfuhl, Andrew 

2OEAMIII4 

Ishihara, Naotada 

S42 

Kubo, Kinya 

1P019 

Ebrahimi, Majid 

2P019 

Ishikawa, Tomoko 

1P062 

Kubota, Hiroshi 

2P050 

Egea, Joaquim 

3OGPMIV2 

Ishizeki, Kiyoto 

1P127 

Kvachnina, Elena 

3OGPMIV2 

Eid, Nabil 

2OHAMII1 

Islam, Ariful 

3P073 

Ladher, Raj 

3P026 

2P112 

Islam, Md. Nabiul 

3P013 

 

3P041 

Eileen, Watson 

3OFPMVI1 

Islam Md, Shafiqul 

S164 

 

2OIAMI3 

Ejiri, Sadakazu 

1P019 

Ito, Chizuru 

S225 

Liu, JunQian 

3P001 

Endou, Yoshio 

2P113 

1OIPM4 

 

1P063 

Farkas, Jozsef 

3P052 

3P064 

Maekawa, Mamiko 

1OIPM4 

Finger, Thomas 

3P053 

Ito, Yuko 

2OHAMII1 

3P064 

Fujii, Hiromi 

1P073 

Itoh, Masahiro 

3P069 

Maimaiti, Kuerban 

2P131 

Fujinaga, Ryutaro 

2P005 

Jahan, Esrat 

1P129 

Mansfield, Carol 

S12 

3P013 

Jahan, Mir Rubayet 

3P013 

Marandi, Nima 

S264

192 

117 

Marson, Lesley 

Masahiro, Itoh 

MasugiTokita, Miwako 

Masuko, Sadahiko 

Matsumoto, Yoshiki 

Matsunaga, Wataru 

Matsuno, Yoshiharu 

Matsuo, Chikahisa 

Matsusue, Yumiko 

Md. Nabiul, Islam 

Mezaki, Yoshihiro 

Miki, Takanori 

Milligan, Carol 

Mir Rubayet, Jahan 

Misawa, Kazuhiko 

Mitani, Kiyoshi 

Mitui, Shinnichi 

Miura, Masahiro 

Miura, Masayuki 

Miura, Mitsutaka 

Miyaso, Hidenobu 

Mizushima, Noboru 

Moreno, Ramon 

Mori, Chisato 

Mori, Nozomu 

Morii, Mayako 

Muhetaerjiang, Musha 

Mukoyama, Yosuke 

Munekazu, Naito 

Murakami, Tohru 

Naganuma, Makoto 

Nagashima, Yu 

Naguro, Tomonori 

Naito, Akira 

Naito, Munekazu 

Nakane, Hironobu 

Nakanishi, Masako 

Nakata, Hiroki 

Ning, Qu 

Nishi, Mayumi 

2OEAMIII4 

2P129 

2P131 

1P031 

2P032 

3P001 

1P002 

3P069 

2P005 

1P002 

1P043 

2P005 

3OFPMVI2 

2P108 

3P001 

S12 

1P043 

2P005 

3P077 

3OGPMVI1 

2P125 

2P113 

S11 

3OFPMVI2 

2P108 

3P069 

S42 

S12 

3P069 

3OGPMIV3 

3OFPMVI2 

2P108 

2P129 

2P131 

S85 

2P129 

2P131 

2P074 

1P073 

3P077 

2P052 

2P061 

1P073 

3P069 

2P052 

2P061 

3P037 

2P098 

2P129 

2P131 

1P002 

Nishimura, Taki 

Niu, Jianguo 

 

Noda, Yasuko 

Oda, Toshiyuki 

Oe, Souichi 

Ogawa, Yuki 

Ohata, Kenji 

Ohno, Nobuhiko 

Ohno, Shinichi 

Ohshima, Hayato 

Ohta, Kenichi 

Ohyama, Kyoji 

OikawaSasaki, Ai 

Oita, Eiko 

Oka, Tatsuro 

Okabe, Shigeo 

Okuda, Hiroaki 

Onga, Kazuko 

Ono, Katsuhiko 

Oppenheim, Ronald 

Otsu, Keishi 

Otsuki, Yoshinori 

Owada, Yuji 

Ozaki, Hiroshi 

 

Park, Jae Hyun 

Parras, C 

Prevette, David 

 

 

Qu, Ning 

QuispeSalcedo, Angela 

Rafiq, Ashiq Mahmood 

Rakwal, Randeep 

Ramadhani, Dini 

Redon, Christophe 

Reza, Mohamad 

Rochefort, Nathalie 

S42 

2P026 

2P041 

3P003 

2P049 

3P003 

3P069 

3P104 

1P004 

1P004 

2P025 

2OGAMII1 

3P001 

3OGPMIV3 

1P127 

S42 

2P026 

1P030 

2P023 

1P020 

1P037 

3OGPMIV3 

1P002 

S12 

1P127 

2OHAMII1 

3P037 

2P019 

3P073 

S164 

1OEPMI1 

1P128 

1P098 

S12 

1P063 

2P130 

3P067 

3P068 

3P069 

2OGAMII1 

1P129 

3OEPMVI2 

1P117 

2P127 

2P128 

3P022 

3ODPMI4 

3ODPMII1 

1OGPMII3 

3ODPMI2 

S264 

Saito, Shouichiro 

Saitoh, Sei 

Saitoh, Yurika 

Sasagawa, Takayo 

Sato, Iwao 

Sato, Toshiaki 

Satoh, Kazuhiko 

Sawada, Tomoo 

Sawaguchi, Akira 

Schwark, Manuela 

Seki, Shinichiro 

Senoo, Haruki 

Setou, Mitsutoshi 

Sharifi, Kazem 

Shibukawa, Yukinao 

Shin, Je Won 

Shinoda, Koh 

Shiomi, Tadahiro 

Shuichi, Hirai 

 

Song, Ning 

Song, Xiaohui 

Sumida, Kaori 

Sunabori, Takehiko 

Sunohara, Masataka 

Suzuki, Katsuhiko 

Suzuki, Shingo 

Suzuki, Takayuki 

Tabata, Makoto 

Tajika, Yuki 

Takada, Masahiko 

Takahashi, Maiko 

Takahashi, Nobuyasu 

Takano, Yoshiro 

Takeshita, Yukio 

Takeuchi, Yoshiki 

Takizawa, Takami 

Takizawa, Toshihiro 

Takumi, Toru 

Tamai, Motoki 

Tanaka, hideyuki 

Tanaka, Shinji 

Taniguchi, Naoyuki 

Tarabykin, Victor 

2P001 

1P004 

1P004 

2P025 

1P002 

2P116 

1P073 

1P019 

2P019 

3P073 

S211 

3OGPMIV2 

3OHPMI1 

3OFPMVI2 

3OIPM4 

2P108 

3P104 

2P019 

1P037 

1P128 

2P005 

3P013 

2P061 

2P129 

2P131 

2P042 

S224 

1P062 

3OHPMI1 

3P023 

2P116 

1P073 

3P001 

3P077 

3OGPMVI1 

2P074 

3OIPM4 

2P074 

S211 

3OGPMVI1 

3P013 

3P001 

1P062 

1P062 

1P030 

3P001 

2P125 

1P030 

1P037 

3OGPMIV2

117 193 

Tatsumi, Kouko 

Terada, Nobuo 

Terada, Sumio 

Terayama, Hayato 

Tokuda, Nobuko 

Toshimori, Kiyotaka 

Toyama, Yoshiro 

Trifonov, Stefan 

Tsuchiya, Reiko 

Tsumori, Toshiko 

Uchida, Takashi 

Uchiyama, Yasuo 

Ueno, Hitoshi 

Urano, Yasuteru 

Velikkakath, Anoop Kumar 

Vinsant, Sherry 

Wada, Yoshinao 

Wakayama, Tomohiko 

Wanaka, Akio 

 

 

Warita, Katsuhiko 

Watanabe, Mineo 

Watanabe, Ryo 

Weinstein, Brant 

Wong, Richard 

 

Yagi, Toshiki 

Yakura, Tomiko 

Yamaguchi, Noriko 

Yamamoto, Miyuki 

Yamano, Mariko 

Yamashita, Kikuji 

Yamatoya, Kenji 

Yanai, Akie 

 

1P020 

1P037 

1P004 

2P025 

3P077 

3P069 

2P019 

3P073 

S225 

1OIPM4 

3P064 

1OIPM4 

3P064 

1OEPMI2 

3P031 

3P104 

2P026 

3P038 

S14 

3P023 

2P074 

2P023 

S42 

S12 

1P037 

2P098 

1P020 

1P037 

3OIPM6 

1P015 

2P020 

3P001 

3P038 

2P108 

S84 

S245 

1OHPM3 

1P015 

2P049 

2P050 

3P001 

2P108 

2P097 

1P020 

3OHPMI1 

S225 

1OIPM4 

3P064 

2P005 

3P013 

1P034 

Yasuda, Kunihiko 

Yasui, Yukihiko 

Yoneda, Toshiyuki 

Yonemura, Yutaka 

Yorifuji, Hiroshi 

Yoshikawa, Kiwamu 

Yoshikawa, Masaaki 

Yoshino, Hiroaki 

 

 

Yuki, Ogawa 

 

 

Zhang, Q 

 

 

 

3P122 

1OHPM2 

3OGPMIV3 

2P026 

3P037 

2P113 

2P074 

3OFPMVI2 

2P108 

S12 

2P108 

1OHPM4 

2P121 

2P131 

3OIPM6 

1P078 3P111 

 

 

 

 

 

3P112 

3P117 

1OFPMII3 

3OIPM6 

S72 

3P128 

1P054 

2P121 

1P098 

3P115 

2P045 3P085 

 

3P088 

3P127 

3P089 

1P056 3P130 

 

 

 

3P078 

2OEAMV2 

1P132 

1P122 1P123 

 

 

 

 

 

 

 

 

 

 

 

2P012 

2P012 

1OGPMII2 

2OFAMIV1 

2OGAMI2 

2OFAMIV1 

1P072 

2OFAMV3 

3OGPMVI2 

2OHAMII2 

2OHAMII3 

2P080 

3P072 

3P113 

2P080 

1OIPM1 

3P100 

1P045 

3P057 

S101 3P015 

3P122 

1P120 

3P055 

2OEAMIII3 

3OHPMIV2 

1P077 3P039 

3P040 3P116 

3P120 3P121 

 

1P087 

2OHAMIII2 

2OFAMIII4 

1P003 

S124 S125 

S163 

2OHAMII3 

2P080 

3OIPM3 

3P100 

2P126 3P132 

 

2OEAMV1 

S117 

3P019 3P030 

2P002 2P010 

1P108 3P018 

3P111 3P112 

3P118 

1OGPMII1 

2OIAMI2 

3OIPMIV2 

1P048 

3P097 

2P103 3P118 

1P044 

2OFAMIII4 

1P093 

3P047 

2OIAMIII1 

1P126 2P083 

2P014 

1OFPMI1 

W15 

2OIAMI3 

2OIAMI4 

2OIAMIII3 

2P092 

1P017 

2P078 3P117

194 

117 

 

 

 

 

 

 

1OFPMI2 

1OFPMI3 

2P058 

2P094 

1OFPMII3 

2P031 

3P071 

3P082 

3OHPMI2 

3P120 3P121 

 

3P094 

W12 3P107 

 

 

 

 

 

 

 

S163 

3P027 

2OGAMI4 

3P034 

3P125 

3P055 

1P009 

S243 1P068 

 

 

2P080 

S192 

1P049 2P082 

 

 

 

3OHPMII1 

3P101 

S103 

1P033 2P033 

 

 

 

 

 

 

2P073 

3P048 

3P128 

1P121 

2OIAMIII3 

S222 

1OFPMI1 

3OHPMII2 

2P072 

3P011 

S94 S165 

 

 

 

2P105 

1P053 

3OHPMIV3 

1P083 

3P122 

1P015 2P020 

 

 

 

 

 

 

3OIPM5 

2OFAMV2 

2P057 

3P066 

1P098 

S41 

1OIPM2 

1OIPM5 

3P111 

3P122 

1OIPM6 

1P064 3P082 

2OEAMV2 

2OHAMIII1 

2P006 

3P118 

1P097 

2P100 2P101 

3P056 

W16 

1OIPM6 

1P064 3P082 

3P072 

2P071 

3P012 

1P047 

3P092 

2P057 

1P050 

S221 3P065 

2OFAMV2 

S83 S241 

S245 

2OFAMIV3 

S161 

2OFAMIV1 

2OGAMII2 

2P109 

1OIPM2 

S151 

2OFAMIII3 

3OIPM3 

2P022 

1OGPMII1 

2OIAMI2 

3OIPMIV2 

1P048 

3P016 

1P040 1P112 

1P074 

1P003 2P003 

2P046 3P050 

3P103 3P110 

3P124 

3P063 

3OFPMVI4 

S94 2P044 

1OHPM5 

3P092 

3P120 

S71 

3P059 

S33 

S223 

1OHPM5 

1P089 2P130 

3P067 3P068 

3P098 

3P090 

1OEPMII2 

2P112 

2P027 

S92 

1OIPM3 

3OFPMVII3 

3P035 

1P093 

2P093 

3P034 

3P043 

S52 

2OIAMIII1 

1P126 

2P066 

1OFPMII2 

S141 1P131 

1OHPM2 

2OHAMI2 

2OIAMI3 

2OEAMV2 

2OHAMIII1 

2P006 

2P045 3P085 

3P088 3P089 

3P095 

3P120 

1P057 

1P017 

3OFPMVI3 

1P059 2P104 

3P022 3P030 

3P052 

S61 

3P062 

1P132 

S72 

2P087 

S112

117 195 

 

 

2P086 

S235 

2P036 3P079 

2P093 3P034 

 

 

 

 

2P017 

S185 

3P078 

3P108 

2P033 3P011 

 

3P014 

1OEPMII3 

2P028 2P107 

 

 

 

 

 

 

2P107 

1OGPMII1 

1P048 

3OGPMVI2 

1P003 

1OHPM4 

2P134 

2P093 3P034 

1P041 3P042 

 

 

 

 

3P021 

2P094 

2P094 

3P130 

S95 1P116 

 

 

 

2P056 

2P075 

1P078 

2P114 

1P040 

2P062 

S23 2P055 

S143 2P036 

 

2P038 

2P121 

3P079 

1P003 2P003 

 

 

2P046 

3P103 

3P124 

3P021 

1P076 

3P050 

3P110 

S63 S74 

 

 

1P066 

2P047 

3P059 

S184 

S111 

1P067 

2P048 

2P127 2P128 

S145 1P129 

 

 

 

 

 

 

1P131 

3P088 

1P132 

1OEPMI3 

2P017 

2P079 

3P072 

3OIPM3 

1P012 

3P085 

3P089 

1P104 1P105 

 

 

 

 

 

 

2P011 

2P060 

3P061 

2OIAMII1 

1P103 

3OHPMIV2 

3P079 

1OFPMI3 

2P058 

2P059 

3P002 

1P060 2P111 

2P119 2P120 

2P124 

3P129 

2P109 2P118 

1P071 2P095 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3OHPMIV4 

1P074 

S51 

3OEPMVI1 

3P049 

3P042 

S53 

S81 

1P069 

3P052 

1P018 

S95 

1OIPM1 

2P085 

2OFAMV2 

1P120 

1OFPMI1 

3P039 3P040 

 

 

 

3OFPMVII3 

3P122 

2P088 

3OEPMVI1 

3P049 

1OIPM6 

1OGPMI4 

3OIPMIV1 

3OIPM6 

S43 

3OFPMVIII1 

2P084 2P089 

3P039 3P040 

3P122 

2OGAMII2 

2OGAMII3 

1OHPM2 

2OEAMIII3 

S162 S213 

W14 1P133 

1P027 1P028 

S123 

1OFPMII3 

3P019 3P020 

3P027 

2P013 3P017 

3P060 

S141 1P129 

1P131 

S81 

2P123 

3OFPMVIII3 

S43 

1P074 

1OHPM1 

1OGPMII3 

2P008 2P070 

2P072 2P094 

2P110 2P112 

1P024 1P025 

1OEPMII2 

2P038 

S252 

S212 2P053 

2P102 3P076 

1P049 

S214 

2P117 

3OFPMVII2 

2OEAMV3 

3P039 3P040 

3P039 3P040 

2P085 

2OIAMII1

196 

117 

3P042 

2OFAMV2 

3OEPMVI1 

3P049 

S45 

2OIAMIII1 

2P083 2P103 

2P126 3P132 

S141 2P041 

1P014 1P015 

2P020 

1P096 

1P024 1P025 

2OEAMIII1 

2P069 

3OFPMVI4 

1P103 

2P022 3P006 

3P007 3P008 

3P108 

1P110 1P118 

1OGPMI3 

2OGAMI3 

S91 1P092 

1P096 2P087 

3P083 

2P042 

1P040 

1P099 

3P122 

- S261 

3P124 

S205 

3OEPMVI2 

3OEPMVIII3 

1P117 2P127 

2P128 

3P022 

S64 

1P097 

2P084 

3P116 

S242 

3OHPMIV3 

1P083 

3P110 

3P026 

1P054 

1P039 

2P027 

 

3OHPMII1 

3P101 

S203 1P033 

1P034 2P033 

 

 

 

 

 

 

 

 

 

 

 

 

2P073 

3P014 

3P125 

1P081 

3P011 

3P048 

3OEPMVIII1 

2OEAMIII4 

2P086 

1OIPM5 

2P071 

S204 

1P098 

3P012 

3P070 

3P123 

3P008 3P108 

 

 

 

1P120 

1P081 

1OFPMII2 

1P092 1P094 

 

 

 

 

 

3P059 

1P115 

2P015 

1OFPMI1 

2P076 3P025 

 

 

1OFPMII3 

3P022 

3P052 

1P111 

3P030 

1P051 1P068 

 

3P122 

1P046 2P091 

1P009 1P010 

 

3P028 

3OHPMIV4 

1P078 

3P112 

3P117 

3P033 

3P111 

3P115 

S202 1P018 

2P031 3P016 

 

 

 

 

 

S132 

3P026 

3P072 

1P111 

3P007 

1P049 2P082 

1P080 

1OEPMI2 

3P031 

3P053 3P054 

3P114 

3P106 

1P064 

S185 

S194 

2P080 

1P113 

1P123 

2P064 

1P092 1P094 

1OFPMI1 

S51 

S105 

1P017 

3OGPMV1 

3P066 

3P125 

2OHAMI2 

1P044 

2P016 

2P007 

S35 

S162 

3P074 

S261 

1OEPMI1 

2P037 2P038 

2P039 2P042 

3P079 

S54 

2OEAMIII2 

1P021 1P080 

3P092 

2OGAMI1 

3OFPMVII2 

3OEPMVII3 

3P101 

S231 

2P015 

3P095 

S261 2P042 

3OGPMV1 

2OEAMIV3 

S23 

2OEAMIV3 

1P023 

2P018

117 197 

 

 

 

 

 

1P082 

2P117 

3P127 

2P029 

S25 

S142 1P072 

 

1OIPM1 

1P009 1P010 

 

3P028 

3P105 

3P033 

S262 2P018 

 

 

 

1P093 

2OEAMV2 

2OHAMIII1 

2P006 

2P012 

1P089 3P098 

1P035 2P021 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3P009 

3P044 

S112 

1P050 

3P043 

3OGPMIV4 

1P036 

1P050 

3P074 

S161 

3P055 

2OHAMI2 

1P103 

1OEPMII3 

2P040 

2P096 

2OHAMI2 

2P094 

 

1P119 2P133 

 

3OIPMIV2 

1P063 3P082 

 

 

 

 

 

 

 

3OIPM3 

1P005 

3P122 

3ODPMI3 

1P097 

3OIPMIV2 

3OEPMVII3 

3P068 

1P092 1P094 

1P096 

 

 

1OHPM4 

2P134 

1OGPMII2 

2OFAMIV1 

2OFAMIV2 

2OGAMI2 

3OEPMVII1 

1P054 

1P060 2P109 

 

2P111 

2P124 

2OHAMI4 

1P070 

2P120 

3P129 

1P102 1P109 

 

 

S92 

1OIPM3 

1OEPMII1 

S181 W13 

 

 

 

 

 

 

2OEAMV1 

S175 

3OIPM6 

1OGPMI4 

2OIAMI2 

2P094 

3P090 

1P080 2P006 

3P092 

W14 3P119 

 

 

 

 

S83 

1OFPMI1 

1OIPM2 

1P069 

2P045 3P085 

- 

 

3P088 

1P036 

S64 

3P089 

3OEPMVIII2 

3P021 

1P102 1P109 

 

2P030 

S223 1P089 

 

 

 

 

 

 

 

 

3P098 

1P072 

S92 

2P126 

3P020 

3OHPMIV3 

2OIAMII3 

2OIAMII3 

1P133 

2P111 

3OIPMIV1 

1P066 1P067 

2P094 

2OIAMIII3 

1P084 

S145 2P045 

3P085 3P088 

3P089 

S251 

2OFAMIII1 

1P097 

3P011 

S254 

2OIAMI1 

1P023 1P071 

2P095 

3P122 

3P122 

3P101 

2P018 

3OHPMI2 

3OHPMIV4 

1P078 3P111 

3P112 3P115 

3P117 

2P123 

3OHPMIV1 

3OIPM2 

2P093 3P034 

3P041 

1OIPM6 

1P052 

S235 

3OFPMVII3 

1OEPMI1 

2P037 2P038 

2P042 

2OEAMIII2 

3P131 

S34 

 

2P130 

3OGPMV3 

1P052 

S112 

1P006 

3P128 

3OHPMII2 

2P072

198 

117 

1P015 2P020 

 

 

 

 

 

 

 

 

 

3OEPMVIII1 

1OGPMII2 

2OFAMIV1 

2OFAMIV2 

2OGAMI2 

1P054 

3OEPMVI2 

2P128 

1P068 

2P120 

1P052 

3P051 

1OGPMI3 

2OGAMI3 

3P028 3P033 

2P011 2P059 

 

 

 

 

 

 

3P061 

3P123 

3P047 

S55 

S233 

3P052 

S101 

S63 S74 

 

 

 

 

 

1P066 

2P048 

3ODPMI4 

1OFPMI4 

1P114 

1P067 

3P059 

3OFPMVII3 

2OGAMI2 

3P122 

3P086 3P087 

 

3OHPMI2 

1P071 2P095 

 

 

 

 

 

 

1P043 

2P123 

3OHPMII1 

S242 

1P005 

3P122 

2P077 3P093 

 

 

3P111 

3P115 

3OFPMVII2 

3P099 

3P112 

S191 1P101 

 

1OEPMII3 

S44 1P053 

2P004 2P068 

2P069 

2OEAMIV3 

1P042 1P045 

2OIAMIII2 

1P052 3P053 

3P114 

1P042 1P045 

S131 

3OHPMIV1 

1OHPM3 

1P069 

3OIPM2 

1P052 3P053 

3P114 

1OFPMII1 

2OGAMIII3 

1P090 2P076 

2P132 3P024 

3P025 3P081 

3P109 

3P020 

2OEAMIII1 

2P009 3P045 

1P048 

3P011 

S161 

2OGAMIII1 

3P026 

S31 

3OEPMVI4 

2P048 

1P120 3P095 

1P015 2P020 

3P102 

3P122 

3P113 

1P116 

3P122 

1P021 1P080 

2P006 3P092 

S95 

3P071 

1OIPM5 

3P086 

3OGPMIV4 

1P036 

1P060 2P106 

1P109 3P035 

3P103 3P124 

 

 

 

 

 

 

 

 

 

3P098 

1P022 

1P029 

3P055 

3P120 

1P100 

1OFPMII2 

2P024 

1P039 

2P028 2P107 

S261 2P042 

 

S91 1P092 

 

 

 

 

 

 

 

 

 

 

1P096 

3P122 

3OIPMIV3 

1P039 

2P080 

1P024 

1P025 

S83 

2OGAMII3 

1OGPMI3 

2OGAMI3 

3P083 

2P002 2P010 

2P053 2P102 

 

 

 

 

3P076 

3OGPMV1 

3OHPMIV2 

1P077 

3OEPMVI2 

1P117 

2P128 

2P127 

3P086 3P087 

 

1P076 

S212 2P053 

2P102 3P076 

 

 

 

 

 

2OFAMIV3 

1P121 

3OFPMVI1 

2P087 

1P121 

W14 3P119 

2P090 3P029 

3P062 

1P084 2P054 

 

 

 

3P131 

2OEAMV2 

1P093 

2P091

117 199 

2OFAMIII2 

1P013 3P004 

3P005 

S192 

3OHPMI2 

3P113 

2P086 

1P126 

1P100 

3P099 

2P073 

3P086 3P087 

2OHAMII2 

3P113 3P128 

2OEAMIII4 

3P043 

2OHAMI3 

2P118 2P119 

1P006 

1OEPMII1 

1P006 

2OFAMIII4 

2OIAMIII1 

1P126 2P083 

3OFPMVIII3 

3P021 

1P016 

3OHPMI2 

1P032 

2OGAMIII2 

1P099 

1P046 1P047 

3OEPMVI3 

2P115 

2OIAMII1 

2P066 2P082 

1P118 

3P074 

1P039 

2P059 2P060 

3P061 

W15 

2OIAMI3 

2OIAMI4 

2OIAMIII3 

2P092 

2OHAMIII2 

2P088 

2OEAMIV1 

3P019 

 

3OIPMIV3 

1P125 3P122 

2P128 3P027 

1P071 2P095 

 

 

 

 

 

 

 

 

3P123 

S24 

3P074 

1P026 

S192 

3OGPMV2 

1P040 1P112 

3P106 

1OHPM3 

2OEAMV2 

2OHAMIII1 

2P006 3P032 

3P092 

S193 1P014 

 

1P015 2P020 

3P102 

2OHAMI2 

1P024 1P025 

 

 

 

 

 

 

 

 

 

S24 

S82 

S173 

3OFPMVI1 

3P057 

2OHAMIII2 

3OIPMIV1 

2OHAMI4 

3P094 

3P113 

1P080 

3P128 

1P117 2P127 

2P128 

1P056 2P033 

 

 

 

3P048 3P130 

1P008 

2OIAMI2 

1P091 

2P126 3P132 

 

 

 

 

 

 

1P061 

3P070 

2P090 

2OHAMI2 

1OHPM4 

2P134 

S54 

 

 

 

 

2P006 

1P069 

3P090 

S61 S202 

1OFPMII3 

3OEPMVI2 

3OEPMVIII3 

1P018 

1P042 

1P055 

2P031 

2P128 

3P019 

3P022 

3P030 

1P026 

1P045 

1P117 

2P127 

3P016 

3P020 

3P027 

3P052 

1P024 1P025 

 

 

 

 

2P027 

3P097 

1P110 

1OGPMII2 

2OFAMIV2 

2OGAMI2 

S101 3P015 

 

 

 

 

2P123 

1P043 

1P026 

S163 

1P003 2P003 

 

 

 

 

3P050 

1OFPMII1 

2OGAMIII3 

3P097 

1P103 

2OFAMIV1 

2P034 2P035 

 

 

 

 

 

 

 

 

 

3OHPMII2 

2P072 

2P112 

3OHPMIV3 

1P042 1P074 

1P083 

1P112 

3OGPMV2 

1P040 

2OGAMIII1 

3OEPMVI2 

1P117 

3P022 

1P039 

2P086 

2P088 

3P106 

2P127

200 

117 

 

 

2OHAMI1 

2OHAMIII3 

1P085 

2P014 

1P118 

1P125 

2P100 2P101 

 

 

3P021 

1OHPM6 

1P088 

3P056 

3P103 3P110 

 

 

 

 

 

1P088 

1P098 

2P118 

3P130 

1P097 

1P024 1P025 

 

 

 

2OFAMIV3 

2P102 

3P094 

1P090 2P076 

 

 

2P132 

3P025 

3P109 

2OIAMI4 

1P069 

3P024 

3P081 

S241 S245 

 

 

 

 

 

 

 

 

 

2OHAMIII3 

1P085 

1OFPMI4 

2OGAMI1 

2P020 

3P118 

2P040 

2OGAMIII2 

3OHPMII1 

3P101 

3OFPMVII2 

3P130 

2P009 3P045 

 

 

S194 

1OFPMII3 

2P099 

1P001 1P038 

 

 

1P023 

3OIPM3 

 

3P086 3P087 

 

 

 

3P058 

1OIPM2 

2OHAMI1 

 

 

 

 

 

 

 

 

 

 

 

 

 

3P058 

2OGAMI3 

1OEPMI2 

3P031 

S152 

S95 

2P038 

2P012 

2OEAMV2 

1P076 

1P056 

2P057 

3OEPMVII1 

W13 

1P027 1P028 

 

 

 

 

 

 

2OHAMII3 

3OIPM3 

2P080 

3P061 

2P089 

1P017 

3P074 

2OFAMV2 

3P100 

1P074 3P084 

3P086 3P087 

 

 

 

S43 

3OFPMVIII1 

1P086 

1P078 

2P103 3P118 

 

 

 

 

 

 

 

2OIAMI4 

S121 

2P115 

3P061 

2P062 

3OIPMIV3 

2OHAMI4 

1P070 

1P009 1P010 

 

 

 

 

 

 

3P028 

1OEPMII1 

3P099 

2P121 

2OGAMIII1 

1P070 

3P033 

S122 1P026 

 

1P104 

1OFPMII3 

3P052 

2P013 3P017 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3P060 

1P038 

S231 

3P099 

2OGAMI4 

1P129 

1P079 

3P053 

3P054 

1P006 

3OIPM5 

3OFPMVI3 

2P104 

1P084 

3P060 

2P027 

2OIAMI1 

1P114 

1P102 1P109 

 

 

 

 

 

3P035 

3P019 

2P008 

1P023 

2P115 

S143 S261 

2P036 2P038 

3P079 

2P009 3P045 

 

 

 

 

1P111 

3OGPMVI2 

3OIPM6 

S72 S73 

 

 

 

 

 

 

 

 

 

 

 

 

 

S75 

3P071 

2OHAMIII2 

3OEPMVIII1 

S81 

3P130 

S53 

2OGAMII2 

1P056 

S153 

3P100 

S73 

1OEPMII3 

1P039

117 201 

3P043 

1P120 

1P097 

2P086 

W14 

2P012 

S81 

3ODPMI1 

1P049 2P082 

2OHAMI2 

3OHPMIV3 

3OIPM2 

1P083 

1OHPM5 

3OEPMVI4 

2OFAMV1 

2P065 

3P119 

2P056 2P062 

2P075 

1P115 

2P086 

2OIAMIII2 

3P118 

2P117 

3P120 

3P051 

1P021 

2OEAMV2 

2OHAMIII1 

2P006 3P092 

S204 

2P045 3P085 

3P088 3P089 

3P058 

3OGPMVI2 

3P113 

3P018 

2OIAMIII1 

1P126 

3P082 

1OIPM6 

1P063 1P064 

3P082 

3OEPMVIII2 

S22 

2P063 

1P033 1P034 

3OEPMVI4 

2OEAMV1 

2P024 

 

 

 

1OEPMII2 

1OHPM5 

1P023 

1P027 1P028 

 

1OIPM6 

3P082 

1P108 3P018 

 

 

 

W12 

1OFPMII2 

2OEAMIV1 

2OEAMIV2 

3OIPM1 

3P107 

3P102 

1P061 

S202 1P018 

 

 

 

 

2P031 3P016 

2P132 

1P098 

2P121 

1OGPMI1 

S95 1P116 

 

 

 

 

 

 

 

 

 

 

 

 

2P056 2P062 

2P075 

1P079 

3OEPMVI1 

3P049 

2P060 

S171 

S92 

1OIPM3 

1P113 

2OFAMIII3 

3OIPM3 

1P005 

S231 

3P055 

3P099 

3P072 

2P013 3P017 

3P060 

1P069 

2OHAMI2 

1P106 

3P130 

2P069 

S201 

S113 

S261 2P038 

S261 

1P069 

 

 

1P132 

1P029 

1P081 1P086 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3P071 

3P124 

2P080 

3OIPMIV3 

3P009 

2OGAMI2 

S162 

1P133 

W15 

2OIAMI3 

2OIAMIII3 

2OFAMV2 

3P119 

2OHAMII3 

3P022 

2OFAMIII2 

3OEPMVI4 

2P106 

2OHAMI1 

2OHAMIII3 

1P085 

3OGPMIV1 

3P022 

S231 

2P059 

2P060 3P061 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2P071 

3ODPMI3 

2P063 

2OHAMIII2 

1P081 

3P123 

1P069 

2P122 

3OEPMVIII3 

1P023 

3P080 

2OIAMI1 

3P063 

3P091 

3ODPMI4 

3ODPMII1 

2OHAMI1 

3P058 

1P024 1P025

202 

117 

 

 

 

 

S36 

1P097 

S95 

S52 

3P022 3P030 

3P052 

3P022 3P030 

 

 

 

 

 

 

 

 

 

3P052 

3OHPMIV3 

1P053 

1OGPMII2 

2P012 

3OFPMVII3 

1P078 

3P117 

2OHAMIII2 

1OHPM2 

3P019 

S141 2P041 

1P003 2P003 

 

 

2P046 

3P103 

3P124 

2OHAMIII2 

1OGPMI4 

3P050 

3P110 

S91 1P092 

 

 

 

 

 

 

 

 

1P096 

3P083 

1P064 

S245 

3P110 

1OGPMII2 

2OFAMIV1 

2OFAMIV2 

2OGAMI2 

3OEPMVII1 

1P054 

3P075 

3P097 

1OEPMII1 

1P006 

2P087 

3P117 

S212 2P053 

 

 

2P102 

1P050 

2OIAMII2 

3P076 

S223 1P089 

 

2P130 

3P068 

S152 

3P067 

3P098 

 

 

 

2P071 

3OEPMVI4 

1P101 

2P030 

1P090 2P076 

 

 

 

 

3P024 

3P081 

3OGPMIV4 

1P087 

1P087 

S194 

3P025 

3P109 

3P093 3P111 

3P112 

3P115 

2P126 3P132 

S162 1P133 

 

 

 

 

 

3OHPMII2 

3P063 

1P026 

1OIPM1 

1P100 

1P081 1P086 

 

 

 

 

3P027 

1OEPMII3 

3P090 

3P063 

3P053 3P054 

3P053 3P054 

 

 

 

2OFAMIV3 

3OIPMIV1 

S161 

 

2P009 3P045 

 

 

2P123 

2OFAMV1 

2P065 

S223 1P089 

 

 

 

2P130 

3P068 

3OEPMVI4 

1P061 

S53 

3P067 

S124 S125 

 

S163 

2OHAMII3 

3OIPM3 

2P080 

3P100 

1P093 1P095 

1P049 2P082 

 

3P036 

2OIAMII1 

2P066 

2OIAMII2 

S25 S133 

3OFPMVII2 

2P047 2P048 

S242 

1P115 

1OGPMI4 

S234 

3OGPMV2 

3P106 

1P101 

S144 1P011 

1P120 3P072 

1OFPMII1 

3P065 

2P109 2P118 

3OHPMI3 

1P041 3P042 

1OGPMI4 

2P093 3P034 

1P097 

1P044 

2P124 

2P111 

2P112 

3P130 

2OEAMIII3 

3OHPMIV2 

1P077 2P037 

3P039 3P040 

3P116 3P120 

3P121 3P125 

3P127 

1OGPMI4 

1P097 

S101 3P015 

1P096 

3P109 

2P123 

2P071 

1OFPMII3 

1P055 3P019 

3P022 3P030 

3P052 

1OGPMII3 

3P118 

S162 S213 

W14 1P133 

3P052

117 203 

 

 

 

 

 

S261 

1OHPM3 

3OHPMII1 

3P101 

1OIPM5 

1OFPMII2 

3P085 3P088 

3P089 

 

 

 

1OHPM1 

1P042 1P055 

2P088 

3P119 

2P100 2P101 

 

 

 

 

3P056 

S102 

1P051 

1P040 

2OHAMII2 

1P090 2P076 

 

 

 

 

3P024 

3P081 

S51 

S182 

3P025 

3P109 

1OFPMII2 

2OEAMIV1 

3P123 

1P081 1P086 

 

 

3P123 

3OEPMVI1 

3P049 

S264 

1P080 3P092 

 

 

 

 

 

 

 

3OHPMIV2 

1P077 

S242 

1OGPMI3 

2OGAMI3 

1P113 

3OEPMVI3 

1P087 

2P090 3P029 

 

 

 

 

 

 

 

3P062 

2OGAMIII1 

3P131 

2OGAMI2 

2P096 

1OGPMI4 

3P092 

3P046 

 

1P029 

2P045 3P085 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3P088 

1P107 

S34 

S161 

3OIPM3 

1P005 

2OHAMII2 

3P089 

2OEAMV3 

2P029 

3P132 

3OGPMV1 

2P040 

2OIAMI2 

1P078 

S192 

- 1P050 

 

 

 

1P058 

2OEAMV3 

2P064 

2P030 

3P058 

1P042 1P055 

 

 

 

 

 

 

 

 

 

 

 

 

1OFPMII2 

3P032 

3OEPMVII2 

S231 

2P018 

1OFPMI4 

2OHAMI2 

1OEPMII2 

2P073 

1OHPM7 

1OHPM3 

S114 3P044 

 

 

S194 

1OFPMII3 

3P127 

S141 1P131 

 

 

3P130 

W15 

2OIAMI3 

2OIAMI4 

2OIAMIII3 

2P092 

1P023 

3OGPMIV2 

1OFPMII2 

1P088 1P131 

2OFAMV2 

3OIPM5 

1P012 

2OIAMII2 

S194 

1OFPMII3 

2P094 

2OFAMV2 

3OHPMI2 

2P071 

1OEPMII3 

1P022 

1P105 

3P047 

1P039 

3P047 

3OEPMVII3 

3P026 

1OFPMI3 

2P013 3P017 

3P060 

1OGPMI1 

1P012 

2P008 

1OEPMII3 

3OHPMII1 

3P101 

3P116 3P120 

3P121 

1OGPMI4 

2P133 

1P021 1P080 

3P092 

1OFPMI3 

3P123 

3OIPMIV3 

S242 

3P041 

1OHPM2 

1P013 3P004 

S251 

2OFAMIII1 

1OHPM2 

2P126 

2OIAMIII1 

1P126 2P083

204 

117 

 

 

 

 

 

 

 

 

 

3OEPMVII2 

1P106 

3P036 

3P124 

1P114 

S261 

1OEPMI1 

2P038 

2P012 

3P006 

1P088 

2P042 

S162 1P133 

 

 

1OGPMI1 

3P062 

1P102 1P109 

 

 

 

3P035 

S53 

2P040 

3OFPMVIII2 

1P077 2P090 

3P029 

S83 S245 

 

 

 

3P057 

3P126 

3OFPMVIII2 

2P062 

S223 1P089 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2P130 

3P068 

3P019 

3P058 

S244 

S53 

S242 

1OIPM2 

1P091 

1OGPMI4 

2OGAMI4 

1P112 

2P027 

3P051 

3P067 

1P010 

S161 

3P090 

2OEAMV1 

2OEAMV3 

2P024 

3P094 

3P067 

3P098 

1P095 

 

 

 

 

 

 

 

 

 

 

 

S253 

3P034 

1P076 

S116 

S186 

1P069 

2OFAMIII4 

3P004 

1P100 

2P099 

3P005 

3P005 

1P009 1P010 

3P028 

3P033 

S134 2P008 

 

 

 

 

 

 

 

 

 

1P052 

S176 

1OEPMI4 

2OEAMV3 

2P058 

3OEPMVI4 

2P120 

1OFPMI2 

2P053 

3P032 

2P084 2P089 

 

 

 

1P006 

S92 

1OIPM3 

S263 

W14 3P119 

 

 

 

 

 

 

 

 

 

3P072 

3P041 

2P078 

2P066 

2OIAMII1 

3OHPMI2 

S84 

1P132 

1P043 

S124 S125 

S163 

2OHAMII3 

3OIPM3 

2P080 

3P100 

S173 S243 

 

 

1P068 

1P102 

S43 

3OFPMVIII1 

S261 2P037 

2P038 2P042 

 

 

 

 

 

 

3P079 

2OFAMIV1 

2OGAMI2 

3ODPMI2 

2P057 

2P084 

3OIPM1 

2P089 

1P108 2P016 

 

 

3P018 

1OHPM3 

3OEPMVIII1 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3P072 

S101 

1P068 

1P022 

3P002 

S261 

1OEPMI1 

2P037 2P039 

3P079 

2P038 

2OHAMIII2 

1OGPMI4 

2OEAMV1 

2P088 

2P121 

3P103 

1OEPMI2 

S63 

3P080 

1OIPM2 

2P031 

3P086 

2P054 

S63 

3OEPMVII2 

1P067 

2P093 

2P004 

2P051 

3OEPMVII3 

3OHPMII1 

3P101 

3P132

117 205 

3P022 3P030 

- 

 

3P052 

3OEPMVI3 

S141 

S94 S165 

 

2P105 

S62 

S165 2P105 

 

 

W15 

2OIAMI3 

2OIAMI4 

2OIAMIII3 

2P092 

2OFAMIV3 

2P034 2P035 

 

3P085 

1P089 2P045 

 

 

 

 

 

3P088 

2P007 

2OEAMV3 

3P063 

2P087 

3P089 

2P013 3P017 

 

 

 

3P060 

1P050 

2P110 

S192 

 

2P021 

1P007 3P041 

 

 

 

 

 

 

 

 

 

 

 

 

1P016 

3P116 

3P015 

1P120 

2OIAMIII2 

2P068 

1P119 

1OGPMI1 

3P090 

1P083 

S194 

1P056 

3P048 3P130 

 

 

 

1P094 

3OEPMVI3 

3P088 

3P009 3P043 

 

3P044 

3P071 

1P090 2P076 

 

 

2P132 

3P025 

3P109 

1OFPMI3 

3OIPM5 

3P024 

3P081 

S145 3P079 

 

3P085 

2OIAMI2 

3P089 

2P127 2P128 

 

 

 

 

 

 

 

3P020 

3OEPMVI3 

S172 

1OHPM4 

2P134 

S93 

1OIPM5 

3P086 

1P069 

1OIPM6 

2P014 

3OIPM3 

S141 1P129 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1P131 

2P078 

2P033 

S81 

1P132 

3P072 

S92 

3P124 

1P039 

1P011 

3OEPMVI4 

3P127 

1P122 

3OHPMII2 

S232 

1P092 1P094 

 

 

 

 

 

 

 

 

 

3P116 

1OEPMI2 

3P031 

2OFAMV2 

1P046 

1P069 

3P075 

3P103 

S32 

1P130 

1P132 

1P068 

3P046 

1P132 

3OFPMVI3 

1P059 2P104 

1OIPM1 

2OHAMIII2 

1P047 

2OFAMV1 

2P065 

2P078 3P117 

1P027 1P028 

 

1OIPM2 

2OHAMIII2 

2P122 3P123 

S53 

1P069 

S53 

1P046 2P091 

3P108 

1P023 

3OEPMVIII2 

1OFPMI1 

2OHAMI4 

1OHPM7 

3P121 

3P094 

3P115 

S184 

2OHAMIII3 

1P085 

1P104 

3OHPMIV2 

1OFPMI3 

3OIPMIV1 

2P043 

S185 

2P126 

2OFAMV2 

1OIPM5 

3P086 

S174 S192 

1P099 

1OGPMI4 

S51 

3P027 

1P060 

3P024 3P081 

3OIPMIV3 

1P125

206 

117 

 

 

1P022 

 

2P130 

 

 

 

 

 

 

 

3OEPMVI2 

1OGPMI4 

2OEAMV2 

2OHAMIII1 

2P006 

1P106 

1P105 

1OGPMI1 

1P086 

S95 1P116 

2P056 

2P075 

2P062 

3P103 3P110 

1P007 3P041 

 

 

 

 

 

 

 

 

 

 

 

 

 

3P128 

3OEPMVI2 

1P117 

2P128 

2P127 

3OFPMVI3 

1P059 2P104 

1OGPMI4 

2P123 

2OIAMIII2 

1OIPM5 

3P086 

3OGPMV3 

3P051 

S92 

2OEAMIII2 

3OFPMVI3 

1P059 

2P104 

1P009 1P010 

3P028 

3P033 

2P109 2P119 

2P120 

3P129 

2P124 

1P102 1P109 

 

 

 

 

3P035 

3P116 

2P117 

1P124 

2P008 

2P124 3P129 

 

 

S262 

3P108 

2P084 2P089 

1P093 1P095 

1P071 2P095 

 

 

 

 

 

 

2OIAMII2 

2P071 

1P021 

3OIPM2 

1P074 

1P049 

3P086 

2P014 

1P015 2P020 

 

 

W11 

2OEAMIII1 

1P016 

3P074 

1P107 

1P027 1P028 

 

 

 

3P041 

S62 

3ODPMI2 

3ODPMI3 

3ODPMI4 

3ODPMII1 

3OEPMVIII2 

S141 S155 

 

 

 

 

 

 

 

 

 

 

 

2P041 

1OGPMI4 

3OGPMV2 

1P040 

S183 

3ODPMI3 

1P043 

1P115 

1P124 

2P081 

1P083 

3P100 

3P100 

3P106 

W14 3P119 

 

 

 

 

 

 

 

3OGPMIV2 

1P115 

1P052 

2OHAMII2 

S115 

1P125 

1OHPM6 

3OHPMII1 

3P101 

 

 

 

 

3P010 

3OFPMVI3 

1P059 2P104 

1P132 

2OFAMIII4 

2P118 2P119 

 

 

 

 

1P070 

3OGPMIV2 

1OEPMI2 

3P031 

1P069 

1P023 1P044 

3P062 

2P090 3P029 

 

3P062 

S194 

1P001 1P038 

1P035 3P009 

2P100 2P101 

 

 

 

 

 

3P056 

1P079 

3OGPMV3 

3P051 

1P124 

3P063 

S104 

2P036 3P079 

 

 

 

 

 

3P114 

3P047 

3P132 

2P079 

3OFPMVIII1 

1P012 1P029 

 

3P024 

1P012 1P029 

 

 

 

 

 

 

 

2OIAMI3 

2OIAMIII3 

1P017 

3OEPMVII3 

3OIPMIV2 

1P003 

1P061 

3OEPMVII3 

1P003 2P003 

2P046 3P050 

 

 

 

3P103 

3P124 

2P134 

2OFAMIV3 

3P110

117 207 

 

 

 

3P010 

1OFPMII3 

1P117 2P127 

2P128 

1P009 1P010 

 

 

 

3P028 

1P113 

1P056 

1OGPMI4 

3P033 

1P047 1P057 

 

 

 

 

 

 

2P041 

3OEPMVIII1 

2P110 

2P092 

2OHAMI2 

1OFPMII1 

2OGAMIII3 

2P111 2P124 

 

 

 

3P129 

1P057 

3OGPMIV4 

1OGPMI1 

3P022 3P030 

 

 

 

 

 

 

 

3P052 

3OFPMVI3 

1P059 2P104 

3OIPM2 

2P027 

2P091 

S154 

3OIPMIV1 

3OFPMVII1 

2P009 3P045 

 

 

 

 

 

 

2P008 

3OEPMVII2 

1P106 

1OGPMI3 

2OGAMI3 

3OIPMIV1 

1P081 

3P041 

S204 

1P059 2P104 

 

 

 

 

1P111 

2P099 

1P130 

2OEAMIV2 

3P107 

S21 S251 

 

 

S201 

2OEAMIV3 

S95 1P116 

 

 

 

 

 

2P056 

2P075 

1OIPM6 

2OIAMII3 

3OGPMIV4 

2P062 

2P076 3P025 

 

 

 

 

 

2P070 

2P021 

2OIAMIII3 

2P092 

1P007 

S162 

1P027 1P028 

 

 

1OGPMII2 

2OFAMIV1 

2OFAMIV2 

2OGAMI2 

1P054 

1P024 1P025 

 

3OGPMV3 

3P051 

S221 3P065 

 

2P007 

2P132 3P024 

 

3P081 

3P131 

S23 W16 

2OEAMIV3 

1P061 

2P055 

S91 1P092 

 

 

 

 

 

 

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