Anti-Cu/Zn SOD - Enzo Life Sciences

Anti-Cu/Zn SOD
TECHNICAL SPECIFICATIONS
5777 Hines Drive, Ann Arbor, MI 48108 USA Toll free (800) 883-8651 Fax (734) 668-2793 www.stressgenbioreagents.com technical@assaydesigns.com
Rabbit Anti-Cu/Zn Superoxide Dismutase Polyclonal Antibody
Product #: SOD-101
Immunogen
Rat Cu/Zn SOD (3).
Specificity
This immunoaffinity purified antibody detects ~23 kDa (human) and
19 kDa (other species) proteins, corresponding to the apparent
molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-
PAGE immunoblots, in samples from human, mouse, rat and bovine
origins.
Species Reactivity
human, mouse, rat, bovine
Applications Certified* Cited*
Western Blot (Colorimetric) (3) 1 μg/mL Yes
Western Blot (ECL) (7,8,9) 1 μg/mL Yes
Immunoprecipitation
10 μg/mL
Immunohistochemistry (6)
1 μg/mL
EIA (3)
Yes
*These working dilutions are provided as suggestions only. Further dilutions may be
possible. Each user should determine the optimal conditions for their own particular
experiment.
Application Note
This antibody is recommended for use with samples of mouse or rat
origin. Stressgen’s product SOD-100 is recommended for use with
samples of human, monkey, hamster, bovine, sheep, rabbit, dog, pig
or Xenopus origins.
Positive Controls
Rat Brain Tissue Extract, Product#: LYT-RB100
Mouse Brain Tissue Extract, Product#: LYT-MB100
Scientific Background
Cu/Zn containing superoxide dismutase (SOD) belongs to the family
of related enzymes which have the same catalytic function but are
structurally different. SOD catalyzes the dismutation of the
superoxide radical O 2 - to O 2 and H 2 O 2 which in turn is metabolized to
H 2 O and O 2 by catalase and glutathione peroxidase (1, 2). The SOD
family is classified into subfamilies on the basis of their metal
cofactors which could be either a dinuclear Cu/Zn or mononuclear
Fe, Mn or Ni. The Fe- and Mn-SODs have identical metal chelating
residues at the active site and share substantial sequence and tertiary
homology, whereas the other SODs are structurally unrelated. The
Cu/Zn-SOD is found in the cytosol of eukaryotes, in chloroplasts and
in the periplasm of some prokaryotes. The eukaryotic, Cu/Zn-SODs
usually consists of two identical subunits of 16 kDa each that are
linked by two cysteines forming an intrasubunit disulphide bridge
and each subunit contains a metal cluster at the active site. The
Cu/Zn-SOD is believed to play a major role in antioxidant defence
mechanisms (3,5). Fe-SOD is present in both aerobic and anaerobic
bacteria, Archaea and plants whereas Mn-SOD is present in bacteria,
Archaea, mitochondria and chloroplasts. In animals, Mn-SOD is
present predominately in the mitochondria as a tetramer of 80 kDa
(3,4). SODs are involved in maintaining the cell integrity and
protecting the functionality of the single cell components against the
damages by oxidative stress (5).
References
1. Bannister, J. V., Bannister, W. H. and Rotilio, G. (1987) Crit. Rev. Biochem. 22: 111-
180.
2. Hassan, H. M. (1988) Free Radical Biol. Med. 5: 377-385.
3. Kurobe, N., Suzuki, F., Kato, K. and Sato, T. (1990) Biomedical Research 11: 187-194.
4. Kurobe, N. and Kato, K (1991) Biomedical Research 12: 97-103.
5. Cannio, R., Fiorentino, G., Morama, A., Rossi, M and Bartolucci, S. (2000) Frontiers in
Bioscience 5: d768-779
6. Xiao-Hong Liu, et al., (1993) Brain Res. 625: 29-37
7. Pedraza-Chaverri, J. et al (2000) Free Radical Biology & Medicine 29(7): 602-611.
8. Katsuki, H. et al (2001) J. Neurochem. 76: 1336-1345.
9. Shinder, G.A., Lacourse, M.-C., Minotti, S. and Durham, H.D. (2001). J. Biol. Chem.
276(16): 12791-12796.
CERTIFICATE OF ANALYSIS
Anti-Cu/Zn SOD
Product #: SOD-101
Lot #: B502510
Size: 100 μg
Rev: 08/12/05
Format: Rabbit immunoglobulin in 0.1M sodium phosphate
buffer (pH7.0), 0.1% NaN 3 , 50% glycerol. This antibody
was purified on an antigen coupled sepharose column.
Concentration: 1.0mg/ml
Host: Rabbit
Antibody Type: Polyclonal
Certification: 1μg/mL of SOD-101 was sufficient for detection of
Cu/Zn SOD in 20μg of rat brain tissue extract (product#
LYT-RB100F) by colorimetric immunoblot analysis
using Stressgen’s Goat anti-Rabbit IgG: AP (product#
SAB-301) as the secondary antibody.
Note: Stressgen makes every effort to provide a consistent source of high
quality polyclonal antisera. However due to variations inherent in this
technology, investigators are urged to purchase sufficient quantities
of a specific lot number if an identical antisera is required throughout
a study.
STORAGE & SHIPPING: Store product at -20°C. Storage below -20°C is not recommended.
Product may be stored for 2-4 weeks at 4°C. For optimal storage, aliquot to smaller portions and
store at -20°C to avoid temperature fluctuations. For maximum product recovery, after thawing,
centrifuge the product vial before removing cap. Shipped on gel packs.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES: This product is not to be used for diagnostic nor
therapeutic purposes. By accepting this product, the user confirms that it
will be used for research purposes only. Stressgen warrants that its
products conform to the information published by Stressgen. Purchaser
must determine the suitability of the product for their particular use.
Please refer to the MSDS for product safety information.

MATERIAL SAFETY DATA SHEET
Section 1 - Product Identification and Use
Product Name: Rabbit anti-Cu/Zn SOD Polyclonal Antibody Product# SOD-101
This product is sold only for research use by qualified laboratory personnel, and is not to be used as a drug, medical device, food additive, cosmetic,
nor household chemical. It is not to be used in diagnostic, therapeutic, consumer, agricultural, nor pesticidal applications.
Manufacturer’s Name:
Stressgen Bioreagents Corporation
Street Address:
350-4243 Glanford Avenue
City, Prov. Postal Code:
Victoria, B.C., CANADA V8Z 4B9
Fax: (250) 744-2877
EMERGENCY PHONE: (250) 744-2866
Section 2 - Hazardous Ingredients
NOT AVAILABLE. We are not aware of any hazards associated with this product or its ingredients, but the chemical, physical, and
toxicological properties of this product have not been investigated thoroughly. Observe normal laboratory precautions.
Section 3 - Physical Data
This product consists of rabbit immunoglobulin in 0.1M sodium phosphate buffer (pH7.0), 0.1% NaN 3 and diluted to 50% in glycerol.
Shipped on gel packs (dry ice overseas). The physical properties of this product have not been investigated thoroughly.
NOT APPLICABLE
NOT APPLICABLE
Section 4 - Fire and Explosion Hazard
Section 5 - Reactivity Data
Section 6 - Toxicological Properties
May be harmful by inhalation, ingestion, or skin absorption. The toxicological properties of this product have not been investigated
thoroughly. Exercise due caution.
Section 7 - Preventative Measures
Wear chemical safety goggles and compatible chemical-resistant gloves. Avoid inhalation, contact with eyes, skin or clothing.
*****MULTIPLE COMPONENT SPILL OR LEAK PROCEDURES*****
• Wear protective equipment.
• Absorb on sand or vermiculite and place in closed containers for disposal.
• Dispose or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and
scrubber. Observe all federal, state and local environmental regulations.
Section 8 - First Aid Measures
• If swallowed, wash out mouth with water, provided person is conscious. Call a physician.
• In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes. If a
rash or other irritation develops, call a physician.
• If inhaled, remove to fresh air. If breathing becomes difficult, call a physician.
• In case of eye contact, flush with copious amounts of water for at least 15 minutes while separating the eyelids with fingers. Call a
physician.
Section 9 - Preparation
Prepared by: Phone#: Created: Modified:
SB Corp. (250) 744-2811 08/12/05 08/12/05
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Stressgen shall not be held liable for any
damage resulting from handling or from contact with the above product. See the Technical Specification, Packing Slip, Invoice, and Product Catalogue for additional
terms and conditions of sale.