Sheet 17 Letter E Lab

e the enormous
lab
Name ___________________
You’ve learned about how the parts of the microscope work. Now, it’s time to use
the microscope to view an image. Today, you'll learn how to prepare a wet mount
slide an view an image under various magnifications.
TO PREPARE A WET MOUNT SLIDE
1. Get your specimen ready. Cut out an “e” from the e
sheet.
2. Use the pipette to place one drop of water on the
center of the slide.
3. Using tweezers, place your specimen (the “e”) onto the drop of water. Be sure that
you can read your “e” and that it is right side up.
4. Gently touch the cover slip to the edge of the slide and lower it to cover your
specimen and the water.
5. Place the slide on stage and position it under the stage clips.
6. On LOW POWER, use the coarse adjustment knob to bring the “e” into focus.
7. Use the fine adjustment knob to sharpen the focus. Draw what you see in the
circle below.
8. How is the letter “e” you see under the microscope different from the way it
normally appears
_____________________________________________________________________
9. While looking through the eyepiece, move the slide to the right.
Which way does the “e” appear to move ___________________________________
10.While looking through the eyepiece, move the slide up and down. How does the
“e” move now ________________________________________________________
11.Rotate the nosepiece to the medium power lens and use the coarse adjustment
then fine adjustment knobs to bring the “e” into focus. Do the same with the high
power lens. Draw what you see below.
SPECIMEN: LETTER “e”
LOW POWER MEDIUM POWER HIGH POWER

PART 2 PROCEDURE:
1. Cut out part of a color newspaper cartoon or a picture from a magazine.
2. Place the section on your slide.
3. Place a drop of water on the paper.
4. Place a cover slip of the picture.
5. Look at the picture under low, medium and high power.
QUESTIONS:
1. What does the diaphragm control
2. What do you notice about the color in the newspaper or magazine
3. Explain why more detail is seen under the high power lens than the low power
lens.
4. Why is it always a good idea to locate a specimen under low power