COURSE TITLE ADVANCED BIOCHEMISTRY (Biokimia Lanjutan ...

Faculty of Resource Science and Technology, UNIMAS
COURSE TITLE
COURSE CODE
ADVANCED BIOCHEMISTRY
(Biokimia Lanjutan)
STB2043
CREDIT 3
PREREQUISITE/S
SYNOPSIS
STB 1083 Biochemistry
This course is a continuation from the basic course, STB1083
Biochemistry. This course is designed to cover topics in the
biochemical reactions within and between the cells that will be
describe in details in this course. Products generated from the
biosynthesis of either amino acids, protein, carbohydrate, fatty
acid or lipids are broken down for the production of energy
either in aerobic or anaerobic processes. Amongst the concepts to
be taught and discussed include a description in details of the
production of energy consists details of all the metabolic
pathways including Glycolysis, TCA cycle or Krebs cycle,
oxidative phosphorylation, photosynthesis and etc. This will be
followed by topics on mechanism of nucleotides with lectures in
biosynthesis of purine and pyrimidine. This is an advanced
biochemistry course that aims to equip students with sufficient
knowledge and information for better understanding of the
biochemical processes and metabolic pathways that involved in
life.
(Kursus ini adalah lanjutan dari kursus asas STB1083 Biokimia.
Ia direkabentuk untuk mencakupi topik-topik berkaitan
tindakbalas biokimia didalam dan diantara sel yang akan
dibincangkan secara lebih mendalam dan terperinci. Hasil akhir
dari biosintesis sama ada asid amino, protein, karbohidrat, asid
lemak atau lipid akan didegradasikan untuk tujuan penghasilan
tenaga baik secara proses aerobik mahupun anaerobik. Antara
konsep-konsep yang akan dipelajari dan dibincangkan
termasuklah kesemua kitaran metobolik penting di dalam
penghasilan tenaga seperti glikolisis, kitaran asid sitrik atau
kitaran Kreb, fosforilasi oksidatif, fotosintesis dan sebagainya.
Ini akan seterusnya diikuti dengan topik mekanisma nukleotida
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dengan syarahan dalam biosintesis purin dan pirimidin. Kursus
ini adalah kursus biokimia lanjutan yang bertujuan untuk
melengkapkan pelajar dengan pengetahuan untuk lebih
memahami tentang proses biokimia dan kitaran metabolik yang
terlibat di dalam kehidupan. )
LEARNING
OUTCOMES
By the end of this course, students will be able to:
o Interpret and compare the chemical processes and the
interactions involved between the living cells in the
production of energy and chemicals through the various
metabolic pathways. (C4, P4)
o Explain the chemical processes and the interactions
involved between the living cells in the production of
energy and chemicals through the various metabolic
pathways. (P2, A3, CT, LL)
STUDENT
LEARNING
HOURS
Lectures
Tutorials
Laboratories/Practical
Student-Centered Learning
Self-Study
Quizzes, Tests, & Examinations
Total
22 Hour/s
2 Hour/s
12 Hour/s
6 Hour/s
72 Hour/s
6 Hour/s
120 Hour/s
LEARNING
UNITS
Supervised
Learning Hours
1. Energy Production I
6
-Glycolysis: details on the degradation including
structures and enzymes
involved.
-Alcoholic fermentation and Pentose Phosphate
Pathway (PPP) as an
alternate path for glucose oxidation.
-TCA (Krebs Cycle) Cycle: detailed description of
pyruvate dehydrogenase
and the production of asetyl-S-CoA and the cycle
including structures and
enzymes and products generated.
-Balancing reaction and regulation: anaplerotic or
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balancing reactions and
regulation of glycolysis and TCA cycle.
-Tutorial & quizzes
-Midterm examination
2. Energy Production II
-Description of the electron transport system and
oxidative
phosphorylation intermediates and stucture
emphasising on the electron
flow and energy release.
-ATP synthesis and chemiosmotic mechanism.
-Photosynthesis: the overall reactions (cyclic and
noncyclic
photophosphorylation).
-The light reaction and generation of ATP and
NADPH
-The dark reactions in both C3 and C4 plants,
gluconeogenesis,acetate
(glyoxcylate cycle), glycogen synthesis and
regulation.
-Tutorial & quizzes
-Midterm examination
9
3. Amino Acid and Nucleotide Metabolism
-Nitrogen cycle and nitrogen fixation
-Nitrogen assimilation involving glitamine and
glycine and three examples
of amino acid synthesis: ser/gly, val, asp/thr/ile.
-Amino acid oxidation and urea production including
transamination:
significant and mechanism, oxidative deamination
and production of
glutamine and its function as nitrogen transporter.
-Nucleotide synthesis and metabolism including:
purine nucleotide
synthesis by the de novo and salvage pathways,
pyrimidine nucleotide
synthesis, nucleotide kinases, pyrophosphorylases,
CTP synthesis and
9
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deoxynucleotide synthesis including dTMP.
-Tutorial & quizzes
4. Applications of Biochemistry
-Application of biochemistry in the field of medicine,
manufacturing, food
production, pharmaceutical and etc.
-Information of biochemistry used and applied in
diagnostic of diseases,
forensic science, manufacture of industrial/cosmetic
products and
agricultural technology development and products,
production of value
added products, wastewater treatments and etc.
-Laboratory demonstration of basic biochemistry
methods such as
preparation of buffers, agarose gel electrophoresis,
SDS-PAGE, protein
purification and basic enzyme kinetics studies.
-Assignment & quizzes
12
5. Enzyme Engineering
-Industrially important enzymes: production, final
purification, cost and
market values.
-Technology of enzyme hybrids and modification,
methods in enzyme
immobilization, secondary metabolite biosynthesis
and their importance
and protein methods such as LC MS/MS and
MALDI-TOF mass spectrometry.
-Assignment & quizzes
6
ASSESSMENT Mid-Semester Examination
25 %
Final Examination
45 %
Assignment:
20 %
Laboratory Report:
10 %
Total
100 %
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REFERENCES 1. Mathews, C. K., Kensal, E., van Holde and Ahern, K.
G.(2000). Biochemistry. 3rd Edition. USA :
Pearson/Prentice Hall
2. Berg, J. M., Tymoczko, J. T. and Stryer L. (2007).
Biochemistry. 6th Edition. USA: W. H. Freeman and
Company.
3. Nelson, D. C., and Cox, M. M. (2004). Lehninger:
Principles of Biochemistry. 4th Edition USA: W. H.
Freeman
4. Voet, D. and Voet, J. G. (2005). Biochemistry. 3rd Edition.
USA: John Wiley and Sons Publication.
5. Horton, R. H., Moran, L. A., Ochs, R. S., Rawn, J. D. and
Scrimgeour K. G. (2006). Principle of Biochemistry 4nd
Edition.
New Jersey, USA: Prentice-Hall International Inc.
6. Cambell, M. K. and Farrell, S. O. (2006). Biochemistry.
Fifth Edition. USA: Thompson Brooks/Cole.
Last updated: May 3, 2011, Tue, 4:25 PM
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