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DupLEX-A Yeast Two-Hybrid Kit - OriGene

DupLEX-A Yeast Two-Hybrid Kit - OriGene

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pNLexA<br />

pNLexA: For fusing LexA to the C-terminus rather than the N-terminus of bait. Contains HIS3 for<br />

selection in yeast, 2 um origin of replication for high copy number in yeast, ADH terminator with<br />

stop codons in all three reading frames, LexA for fusion protein, polylinker for subcloning of bait<br />

gene, ADH promoter for constitutive expression of fusion protein in yeast, and Amp R for selection in<br />

E. coli.<br />

Revision 06.05 - 20 - Your Gene Company

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