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FIGURES Fig. 1: Recommended gating during data acquisition, data are acquired by using fluorescence triggering in the FL3 channel (red fluorescence > 630 nm). Region 1 set on leukocytes and counting beads. A) Region 1set on leukocytes and counting beads, negative control sample B) Region 1 set on leukocytes and counting beads, positive control sample 1 A 1 B Fig. 2: Typical dot plot (SSC/FSC) display during data acquisition and analysis, gate set on region 1 Region 2 set on counting beads, region 3 set on granulocytes A) Region 2 set on counting beads, region 3 set on granulocytes, negative control sample B) Region 2 set on counting beads, region 3 set on granulocytes, positive control sample Note: Shape change of the granulocytes (FSC-H signal)! 2 A 2 B For reseach use only. Not for therapeutic or in vitro diagnostic use. Page - 5 - Revised 6/02
Fig. 3: FL1 histograms during data analysis, gate set on combination of region 1 and 3 A) FL1 histogram of the granulocyte population in region 3, LECAM-1 control sample B) B) FL1 histogram of the granulocyte population in region 3, test sample Note: Increase of the percentage of activated cells showing a reduced expression of LECAM-1. FL1 corresponds to green fluorescence (bandpass filter 530 +/- 15 nm) 3 A 3 B Fig. 4: Kinetics of chemotaxis by neutrophil granulocytes At different time intervals the number of granulocytes was determined which migrated through cell culture inserts. For reseach use only. Not for therapeutic or in vitro diagnostic use. Page - 6 - Revised 6/02
Page 2 and 3: SUMMARY and EXPLANATION This test a
Page 4 and 5: Data evaluation: After setting a lo
Page 8: For reseach use only. Not for thera

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