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The fourth exercise for year 2005 - NASCOLA

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<strong>NASCOLA</strong><br />

Executive Committee<br />

<strong>2005</strong>-2006<br />

Officers<br />

President<br />

Alvin H Schmaier, MD<br />

University of Michigan<br />

Ann Arbor, MI<br />

aschmaie@umich.edu<br />

Immediate Past President (ex officio)<br />

William L Nichols, MD<br />

Mayo Clinic<br />

Rochester, MN<br />

nichols.william@mayo.edu<br />

Vice-President<br />

Ellinor I B Peerschke, PhD<br />

Weill Medical College-Cornell University<br />

New York City, NY<br />

epeersch@med.cornell.edu<br />

Secretary<br />

Elizabeth Van Cott, MD<br />

Massachusetts General Hospital<br />

Boston, MA<br />

evancott@partners.org<br />

Treasurer<br />

John L Francis, PhD<br />

Florida Hospital Cancer Institute<br />

Orlando, FL<br />

John.Francis@flhosp.org<br />

Members-At-Large<br />

Catherine P M Hayward, MD, PhD<br />

McMaster University Medical Centre<br />

Hamilton, Ontario, Canada<br />

haywrdc@mcmaster.ca<br />

Elizabeth Plumhoff, MT(ASCP),CLS (NCA)<br />

Mayo Clinic<br />

Rochester, MN<br />

plumhoff.elizabeth@mayo.edu<br />

James Zehnder, MD<br />

Stan<strong>for</strong>d University<br />

Stan<strong>for</strong>d, CA<br />

zehnder@stan<strong>for</strong>d.edu<br />

Executive Officers<br />

2003-2007 (ex officio)<br />

Director, Quality Assessment Program<br />

Richard A Marlar, PhD<br />

Oklahoma City VA Medical Center<br />

Oklahoma City, OK<br />

Richard-Marlar@ouhsc.edu<br />

<strong>NASCOLA</strong> Website<br />

www.nascola.org<br />

Contact<br />

info@nascola.org<br />

November 10, <strong>2005</strong><br />

Dear <strong>NASCOLA</strong> Colleagues:<br />

<strong>The</strong> <strong>fourth</strong> <strong>exercise</strong> <strong>for</strong> <strong>year</strong> <strong>2005</strong> (<strong>2005</strong>-4) of the <strong>NASCOLA</strong>/ECAT External<br />

Quality Assessment Program <strong>for</strong> Hemostasis and Thrombosis has be<br />

shipped this week via Federal Express.<br />

Page two of this correspondence contains all instructions necessary <strong>for</strong><br />

sample handling. Please review carefully be<strong>for</strong>e proceeding with storage<br />

and reconstitution.<br />

On the web site (www.nascola.org) you will find, in addition to this<br />

document, the Methods and Instrument List <strong>for</strong> <strong>2005</strong> Survey<br />

Program (from which you will choose codes <strong>for</strong> test methods and<br />

instrumentation) and How to Report Survey Results via the Internet<br />

(This Web Site). All laboratories will report results via our web site. You<br />

will not receive any paper report <strong>for</strong>ms. Laboratory codes (3 digits) and<br />

password (8 digits) were distributed to all members <strong>for</strong> the first survey of<br />

<strong>2005</strong> and these have not changed. <strong>The</strong> deadline <strong>for</strong> internet reporting<br />

is December 12, <strong>2005</strong>.<br />

As a reminder, should assays appear as on-line choices that do not match<br />

those that you ordered/received, please remember that <strong>NASCOLA</strong> does<br />

not subscribe to all modules provided by ECAT. Only those samples<br />

denoted on page two are ones to which we subscribe.<br />

Should you have any issues regarding this survey, please immediately<br />

contact Dr Marlar or Melissa Goodsell in his laboratory. On behalf of the<br />

<strong>NASCOLA</strong> Central Laboratory (Oklahoma City VA), we wish you our best<br />

and hope you enjoy the Exercise!<br />

Richard Marlar, PhD<br />

Melissa Goodsell<br />

Director, QA Program, <strong>NASCOLA</strong> QA Program, <strong>NASCOLA</strong><br />

Oklahoma City VA Medical Center Oklahoma City VA Medical Center<br />

Ph: 405-270-0501 Ext 5261 405-270-0501 Ext 4189<br />

Richard-Marlar@ouhsc.edu<br />

Rev6: 10Nov05


<strong>NASCOLA</strong><br />

Executive Committee<br />

<strong>2005</strong>-2006<br />

Officers<br />

President<br />

Alvin H Schmaier, MD<br />

University of Michigan<br />

Ann Arbor, MI<br />

aschmaie@umich.edu<br />

Immediate Past President (ex officio)<br />

William L Nichols, MD<br />

Mayo Clinic<br />

Rochester, MN<br />

nichols.william@mayo.edu<br />

Vice-President<br />

Ellinor I B Peerschke, PhD<br />

Weill Medical College-Cornell University<br />

New York City, NY<br />

epeersch@med.cornell.edu<br />

Secretary<br />

Elizabeth Van Cott, MD<br />

Massachusetts General Hospital<br />

Boston, MA<br />

evancott@partners.org<br />

Treasurer<br />

John L Francis, PhD<br />

Florida Hospital Cancer Institute<br />

Orlando, FL<br />

John.Francis@flhosp.org<br />

Members-At-Large<br />

Catherine P M Hayward, MD, PhD<br />

McMaster University Medical Centre<br />

Hamilton, Ontario, Canada<br />

haywrdc@mcmaster.ca<br />

Elizabeth Plumhoff, MT(ASCP),CLS (NCA)<br />

Mayo Clinic<br />

Rochester, MN<br />

plumhoff.elizabeth@mayo.edu<br />

James Zehnder, MD<br />

Stan<strong>for</strong>d University<br />

Stan<strong>for</strong>d, CA<br />

zehnder@stan<strong>for</strong>d.edu<br />

Executive Officers<br />

2003-2007 (ex officio)<br />

Director, Quality Assessment Program<br />

Richard A Marlar, PhD<br />

Oklahoma City VA Medical Center<br />

Oklahoma City, OK<br />

Richard-Marlar@ouhsc.edu<br />

<strong>NASCOLA</strong> Website<br />

www.nascola.org<br />

Contact<br />

info@nascola.org<br />

Rev6: 10 Nov 05<br />

EXERCISE <strong>2005</strong>-4<br />

CLOSING DATE December 12, <strong>2005</strong><br />

WARNING<br />

<strong>The</strong> plasma samples have been tested by an FDA approved method <strong>for</strong> the presence of HIV antigen as well as <strong>for</strong><br />

hepatitis B surface antigen and have been found to be negative. However, as with all preparations of human<br />

origin, suitable precautions should be taken in their handling and disposal.<br />

STORAGE AND STABILITY<br />

Unreconstituted lyophilised plasma should be stored at 2-8°C or lower.<br />

Reconstituted plasma should preferably be used within 1 hour after reconstitution. Plasma should be stored at 2-<br />

8°C after reconstitution. For immunological methods the reconstituted plasma can be stored <strong>for</strong> 1 month at –<br />

20°C.<br />

RECONSTITUTION<br />

For proper reconstitution the vial must reach room temperature be<strong>for</strong>e adding the water. Dissolve the contents of<br />

each vial in sterile, distilled, room temperature water. For the exact volume of water to be used: see table below.<br />

Leave the vial <strong>for</strong> 5 minutes. Swirl the vial gently to mix and leave <strong>for</strong> a further 15 minutes <strong>for</strong> complete reconstitution. Be<strong>for</strong>e<br />

use mix the vial again gently.<br />

Sample Code Volume (ml) Test<br />

05.46 0.5 Antithrombin<br />

05.47 0.75 Protein C activity and antigen<br />

05.48 1.0 Protein S activity, total and free antigen<br />

05.49 1.0 Activated Protein C Resistance Ratio<br />

05.51 (2 vials) 1.0 (per vial) Lupus Anticoagulant<br />

05.54 (2 vials) 1.0 (per vial) Coagulation Factors VIII, IX, XI and XII<br />

05.55 (2 vials) 1.0 (per vial) Coagulation Factors VIII, IX, XI and XII<br />

05.56 (2 vials) 1.0 (per vial) Coagulation Factors II, V, VII and X<br />

05.57 (2 vials) 1.0 (per vial) Coagulation Factors II, V, VII and X<br />

05.60 (2 vials) 1.0 (per vial) Von Willebrand Factor<br />

<strong>The</strong> samples you receive depend on the test(s) on which you are registered<br />

Special in<strong>for</strong>mation <strong>for</strong> users of Latex Immuno Assays<br />

Users of Latex Immuno assays should centrifuge the samples after reconstitution <strong>for</strong> at least 10 minutes<br />

at 10.000 x g (or higher speed).<br />

LISTS WITH CODES FOR METHODS AND EQUIPMENT<br />

<strong>The</strong> lists with the codes <strong>for</strong> the methods and equipment used were provided separately in February.<br />

This list will not be provided anymore with each survey. If you need an additional copy, please e-<br />

mail to P.Meijer@ecat.nl<br />

REPORTING RESULTS: Did you <strong>for</strong>get your For internet result submission, you don’t<br />

receive result <strong>for</strong>ms anymore!!!<br />

Did password? Please e-mail Richard Marlar (Richard-Marlar@ouhsc.edu) or William Nichols<br />

(nichols.william@mayo.edu).

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