Analyst® 1.6.1 Software Automatic Optimization Tutorial - AB Sciex

Automatic Optimization Tutorial
Table 1-7 Product Ion Auto Selection Criteria Dialog Parameters (Continued)
Parameter Value Description
Exclude Product
Ions within ± (Da of
Precursor Ion m/z)
Min. Mass for
Product Ion (amu)
Threshold for
Product Ion (cps)
20.000 The Da value that defines the exclusion window
around the precursor ion so that fragment ions
that fall within this window are not selected for
MRM optimization. For example, if you type ± 5
Da for a precursor ion of 500 m/z, any fragment
ions within the 495 to 505 m/z region are
excluded. This prevents the precursor ion from
being optimized.
60.000 The lowest fragment mass to be considered for
optimization. Use this option to narrow or widen
the window of fragment ions to be considered
from the precursor mass.
100.000 Minimum number of counts for a product ion to
be considered.
7. Click OK to save the changes to the selection criteria.
8. Click Next.
9. In the Target Components dialog, type the parameters from the following table.
Note: The compound name must be unique for each compound or
transition.
Table 1-8 Target Compounds Dialog Parameters
Target Compound Field Value*
Reserpine Compound Name Reserpine
MW (Da) 609.4
No. Charges 1
Minoxidil Compound Name Minoxidil
MW (Da) 210.2
No. Charges 1
Tolbutamide Compound Name Tolbutamide
MW (Da) 271.3
No. Charges 1
Rescinnamine (IS) Compound Name Rescinnamine
MW (Da) 635.4
No. Charges 1
* You must type the exact ion mass.
10. Click Finish to begin the optimization process.
Analyst ® 1.6.1 Software
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Automatic Optimization Tutorial
Release Date: March 2012