Analyst® 1.6.1 Software Automatic Optimization Tutorial - AB Sciex
Analyst® 1.6.1 Software Automatic Optimization Tutorial - AB Sciex
Analyst® 1.6.1 Software Automatic Optimization Tutorial - AB Sciex
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<strong>Automatic</strong> <strong>Optimization</strong> <strong>Tutorial</strong><br />
Use infusion optimization to optimize compound-dependent parameters only at much lower flow<br />
rates than those used during LC/MS analysis.<br />
FIA<br />
FIA is the injection of a sample by the autosampler into the mass spectrometer using LC. During<br />
the FIA optimization process, multiple sample injections are performed for various ion source- or<br />
compound-dependent parameter types that are changed between injections. FIA compound<br />
optimization optimizes parameters by performing looped experiments in succession, that is, one<br />
compound- dependent parameter is optimized first followed by the next compound-dependent<br />
parameter. It optimizes for ion source-dependant parameters by making one injection for each<br />
value.<br />
Compound parameters need to be narrowed down using at least two more FIA cycles. Use FIA<br />
optimization to optimize both compound-dependent and source-dependent parameters using LC<br />
at higher flow rates.<br />
Table 1-1 Differences Between Sample Introduction Methods<br />
Method Required devices Parameters Typical flow rate range<br />
Infusion Syringe pump Compound-dependent 5 to 25 µL/min<br />
FIA LC pump and<br />
Autosampler<br />
Source- and compounddependent<br />
25 to 1000 µL/min<br />
During optimization, a text file is generated and then displayed during the optimization process.<br />
This file records the various experiments performed and the optimal values for each parameter. A<br />
file folder containing all the data files is also generated. For each experiment performed, an<br />
acquisition method is also generated and saved in the Acquisition Methods folder.<br />
Related Documentation<br />
• Getting Started Guide for the Analyst ® software<br />
• Advanced User Guide for the Analyst software<br />
• The Analyst software Help system<br />
• The applicable ion source guide<br />
Technical Support<br />
<strong>AB</strong> SCIEX and its representatives maintain a staff of fully-trained service and technical<br />
specialists located throughout the world. They can answer questions about the instrument or any<br />
technical issues that may arise. For more information, visit the Web site at www.absciex.com.<br />
Prerequisites<br />
• The instrument is tuned and calibrated.<br />
• An acquisition method.<br />
Analyst ® <strong>1.6.1</strong> <strong>Software</strong><br />
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<strong>Automatic</strong> <strong>Optimization</strong> <strong>Tutorial</strong><br />
Release Date: March 2012