ZR-75-1
ZR-75-1
ZR-75-1
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SOP: Thawing, Propagation and Cryopreservation of NCI-PBCF-CRL1500<br />
(<strong>ZR</strong>-<strong>75</strong>-1)<br />
APPENDIX 2: GROWTH PROFILE OF NCI-PBCF-CRL1500 (<strong>ZR</strong>-<strong>75</strong>-1) CELLS.<br />
7.00E+05<br />
6.00E+05<br />
5.00E+05<br />
Viable Cells/cm 2<br />
4.00E+05<br />
3.00E+05<br />
2.00E+05<br />
1.00E+05<br />
0.00E+00<br />
0 2 4 6 8 10 12<br />
Days in Culture<br />
Figure 4: Growth curve for <strong>ZR</strong>-<strong>75</strong>-1 cells; cells were plated at 5 x 10 4 viable<br />
cells/cm 2 ; population doubling time (PDT) is approximately 54 h.<br />
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