ZR-75-1

More documents

Recommendations

Info

SOP: Thawing, Propagation and Cryopreservation of NCI-PBCF-CRL1500 (ZR-75-1) APPENDIX 4: GLOSSARY OF TERMS Confluent monolayer: adherent cell culture in which all cells are in contact with other cells all around their periphery and no available substrate is left uncovered. Split ratio: the divisor of the dilution ration of a cell culture to subculture (e.g., one flask divided into four, or 100 mL up to 400 mL, would be split ratio of 1:4). Subculture (or passage): the transfer or transplantation of cells, with or without dilution, from one culture vessel to another. Passage No: the total number of times the cells in the culture have been subcultured or passaged (with each subculture the passage number increases by 1). Population doubling level (PDL): the total number of population doublings of a cell line since its initiation in vitro (with each subculture the population doubling increases in relationship to the split ratio at which the cells are plated). See Appendix 7. Population doubling time (doubling time): the time interval, calculated during the logarithmic phase of growth in which cells double in number. Seeding density: recommended number of cells per cm 2 of substrate when inoculating a new flask. Epithelial-like: adherent cells of a polygonal shape with clear, sharp boundaries between them. Fibroblast-like: adherent cells of a spindle or stellate shape. Page 20 of 24
SOP: Thawing, Propagation and Cryopreservation of NCI-PBCF-CRL1500 (ZR-75-1) APPENDIX 5: REFERENCE 1. Culture Of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 6th edition, published by Wiley-Liss, N.Y., 20105. 2. 1230: Engel LW, Young NA. Human breast carcinoma cells in continuous culture: a review. Cancer Res. 38: 4327-4339, 1978. PubMed: 212193 3. 23031: Engel LW, et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 38: 3352-3364, 1978. PubMed: 688225 4. 23102: Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822 5. 25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 6. 32268: Couillard S, et al. Comparison of the effects of the antiestrogens EM-800 and tamoxifen on the growth of human breast ZR-75-1 cancer xenografts in nude mice. Cancer Res. 58: 60-64, 1998. PubMed: 9426058 7. 32273: Wang E, et al. Posttranscriptional regulation of protein expression in human epithelial carcinoma cells by adenine-uridine-rich elements in the 3'-untranslated region of tumor necrosis factor-alpha messenger RNA. Cancer Res. 57: 5426-5433, 1997. PubMed: 9393771 8. 32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 9. 32288: Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562 3568, 1997. PubMed: 9270029 10. 32341: Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194 Page 21 of 24
Page 1 and 2: NCI-PBCF-CRL1500 (ZR-75-1) Breast C
Page 3 and 4: SOP: Thawing, Propagation and Cryop
Page 19: SOP: Thawing, Propagation and Cryop
Page 23 and 24: Table 5: Cell Expansion FROM FLUID

ZR-75-1

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?