hTERT-HME1
hTERT-HME1
hTERT-HME1
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SOP: Thawing, Propagating and Cryopreserving NCI-PBCF-CRL4010 (<strong>hTERT</strong>-<strong>HME1</strong>)<br />
cells<br />
APPENDIX 3: CYTOGENETIC ANALYSIS OF HTERT-<strong>HME1</strong> CELLS<br />
NCI-PBCF-CRL4010 Karyotype Results<br />
Metaphase Spread<br />
Karyotype<br />
Number of metaphase spreads counted 20<br />
Band level 300-400<br />
Number of metaphase spreads karyotyped 10<br />
Chromosome range 44-46<br />
Sex<br />
Comments<br />
Female<br />
Aneuploid*<br />
Karyotype:<br />
44-46,XX,-X,i(10)(q10),add(12)(q24.1),-17,-18,+1-3mar<br />
(ISCN nomenclature written based on a diploid karyotype).<br />
*Human diploid karyotype (2N): 46,XX (female) or 46,XY (male)<br />
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