Med Entomol Zool 65 151-155

Med. Entomol. Zool.Vol. 65No. 3p. 1511552014 151
DOI: 10.7601/mez.65.151
*
8528523 1124
201426 201451
Experiment on mosquito blood feeding using the artificial feeding device
Chiaki Tsurukawa and Hitoshi Kawada*
* Corresponding author: Vector Ecology & Environment, Institute of Tropical Medicine, Nagasaki University,
Sakamoto 1124, Nagasaki 8528523, Japan (E-mail: vergiss@nagasaki-u.ac.jp)
Vector Ecology & Environment, Institute of Tropical Medicine, Nagasaki University,
Sakamoto 1124, Nagasaki 8528523, Japan
(Received: 6 February 2014; Accepted: 1 May 2014)
Abstract: Experiments on blood feeding of Aedes aegypti using the artificial membrane feeding system (Hemotek
Ltd., Blackburn, UK), selection of good animal bloods and membranes, which were commercially available from
reagent manufacturers or food companies, as good experimental materials for artificial membrane feeding of
mosquitoes, were carried out. Among the 3 kinds of preserved bloods (chicken, bovine, and equine), the equine
defibrinated blood, and the equine hemolyzed blood, the preserved bloods gave the best feeding results. Natural
porcine gut for sausage casing was the most successful membrane as compared to the two collagen films and
Parafilm ® . Combinational use of the bovine preserved blood and the porcine gut membrane gave good feeding
results for Ae. aegypti and Anopheles stephensi.
Key words: artificial membrane feeding, Aedes aegypti, Anopheles stephensi, preserved blood, porcine gut
animal welfare
186
17 68
3RRefinement
Replacement Reduction
3
Hemotek Hemotek
5WIB 100
Aedes
aegypti L. Anopheles stephensi Liston
1.
Hemotek Membrane Feeding SystemHemotek Ltd., Blackburn,
UK HMFS
Meal reservoir
Feeding UnitFeeding Unit 5
Cosgrove et al. 1994; Fig. 1
2.
Aedes aegypti L. 2000
3.
HMFS
5
Table 1 HMFSMeal Reservoir5
34 ml
Fig. 1-1
Fig. 1-237.5°C
20 cm×20 cm×30 cm5
510 50
70 5Feeding Unit

152 Med. Entomol. Zool.
2
20 ml
1
3%
50 ml
25°C75%, 16L8D 711
15
1
4.
Table 24
Meal Reservoir
Meal Reservoir
Feeding Unit
50 5
2
3
2
Fig.1.Photo of Hemotek Membrane Feeding System.
Feeding unit with meal reservoir equipped with blood sample
and membrane (Fig. 1-1). Four feeding units put on mosquito
cages and connected to temperature controller (Fig. 1-2).
5.HMFS Anopheles stephensi
1 An. stephensi
50 Feeding Unit2
Table1.List of Animal blood samples tested for Hemotek Membrane Feeding System.
Blood Samples Supplier Price (/100 ml)
Term of Validity after
Production
Equine Blood, Preserved Nippon Bio-Test Laboratories Inc. 1) 2800 yen 4 wk at 28°C
Bovine Blood, Peserved Nippon Bio-Test Laboratories Inc. 9200 yen 4 wk at 28°C
Chicken Blood, Preserved Nippon Bio-Test Laboratories Inc. 6500 yen 2 wk at 28°C
Equine Blood, Defibrinated Nippon Bio-Test Laboratories Inc. 3800 yen 2 wk at 28°C
Equine Blood, Hemolyzed Nippon Bio-Test Laboratories Inc. 6500 yen 1 yr at − 20°C
1) http://www.nbiotest.co.jp/product/product5.html
Table2.List of Membrane samples tested for Hemotek Membrane Feeding System.
Membrane Samples Supplier Use Purpose Price
Porcine gut (Salted) 1) Garden Cook 3) Casing for sausage 1150 yen (2 m×3)
Collagen Casing (Artificial) 2) Ma’am Co., Ltd. 4) Casing for sausage 970 yen (12 m)
Parafilm ® As One Co., Ltd. 5) Laboratory use
Collagen Membrane (Artificial) Hemotek Ltd. Accessory of HMFS 1800 yen (1 m×0.4 m)×5
1) http://www.garden-cook.com/products/120002nc.html
2) http://www.ma-am.jp/shop/shop.cgi?id=1586
3) http://www.garden-cook.com/index.html
4) http://www.ma-am.jp/shop/
5) http://www.as-1.co.jp/

Vol. 65No. 32014 153
2
6.
1
Rlme4
R multcomp Tukey’s HSD-test
arcsin
1.
5Fig. 2
27.7
8.35.30.47.462
4
p>0.05; Fig. 2-1
5.16.72.504.9
p0.05
41
44.4, 57.1, 45.3, 43.6
p>0.05;
Fig. 2-3 72.0%, 87.1%, 82.0%, 64.9%
4 p>0.05;
Fig. 2-43
70.3%,
87.3%, 74.4%
15 1
92.9, 81.5%, 84.0%
2.
4Fig. 3
HMFS
204.5 10.5 34.0
p

154 Med. Entomol. Zool.
Fig.3.Number of blood-fed females, number of oviposited females, number of eggs per female, and hatchability of eggs oviposited by
Aedes aegypti with different types of membrane by artificial membrane feeding with Hemotek Membrane Feeding System. The bovine
preserved blood was used as blood meal. Boxes and bold lines indicate the 1st and 3rd quartiles, and medians, respectively. Different
letters indicate significant difference by Tukey’s HSD test (p0.05; Fig. 3-3
65.0%, 92.0%, 86.2%
HMFS
p

Vol. 65No. 32014 155
Phasomkusolsil et al., 2013Hagen and Grunewald,
1990; Cosgrove et al., 1994Kogan, 1990
Novak et al., 1991; Tseng,
2003; Phasomkusolsil et al., 2013
Phasomkusolsil et al. 2013
HMFS
4
HMFS
Kogan, 1990; Kasap et al., 2003; Tseng,
2003; Mishra et al., 2005; Nasirian et al., 2006
Cosgrove et
al., 1994; Cosgrove and Wood, 1995 Novak et al. 1991
4
Phasomkusolsil et al. 2013
An. stephensi
Kogan, 1990; Cosgrove and Wood, 1995
γ-
ATP
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