DETERGENTS AND THEIR PROPERTIES BEN HANKAMER ... - 2dx
DETERGENTS AND THEIR PROPERTIES BEN HANKAMER ... - 2dx
DETERGENTS AND THEIR PROPERTIES BEN HANKAMER ... - 2dx
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A PARTICULAR TEMPERATURE ABOVE THE CMT, AT WHICH NON-IONIC<br />
<strong>DETERGENTS</strong> UNDERGO PHASE SEPARATION TO YIELD A DETERGENT RICH <strong>AND</strong> A<br />
DETERGENT POOR AQUEOUS PHASE.<br />
CAUSED BY DECREASED HYDRATION OF THE HEADGROUP.<br />
E.G.<br />
TRITON X-100 CLOUD POINT = 64 o C<br />
TRITON X-114 CLOUD POINT = 22 o C<br />
CLOUD POINT<br />
USES: MEMBRANE PROTEINS CAN BE SOLUBILIZED AT 0 o C, BEFORE BEING<br />
PURIFIED FROM SOLUBLE PROTEINS BY WARMING TO 30 o C, SO THAT TWO PHASES<br />
ARE FORMED, WITH THE MP BEING ENRICHED IN THE DETERGENT RICH PHASE.
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