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Metabolic Engineering of Carbohydrates - (CUSAT) – Plant ...

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Genetic manipulation:<br />

• A number <strong>of</strong> transgenic plants producing<br />

polyfructans have been developed<br />

• In earlier experiments the sacB gene <strong>of</strong> B.<br />

subtilis or B. amyloliquifaciens which encodes<br />

a levan-sucrase catalyzing a 6-2β linkage was<br />

transformed into maize, tobacco, potato,&<br />

sugarbeet.<br />

• sacB gene was modified with a vacuolar<br />

targeting sequence from the yeast<br />

carboxypeptidase gene(cpy).

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