Lymphocyte proliferation assay - Immunopaedia
Lymphocyte proliferation assay - Immunopaedia
Lymphocyte proliferation assay - Immunopaedia
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3H-thymidine incorporation <strong>assay</strong><br />
Stimulus<br />
Cell cytoplasm<br />
3 H-thymidine<br />
Cell nucleus<br />
Proliferation<br />
Chromosomal<br />
DNA<br />
Cell nucleus<br />
PREVIOUS<br />
Proliferation<br />
BACK TO<br />
START<br />
The 3H-thymidine incorporation <strong>assay</strong> is used to determine the extent of cell division in response to a <strong>proliferation</strong> signal such<br />
as via stimulation with a mitogen or antigen. <strong>Lymphocyte</strong>s are cultured in the presence of the stimulus and 3H-thymidine which<br />
is a radioactive nucleoside and a precursor of thymine found in DNA. Following activation, proliferating cells replicate their<br />
chromosomal DNA and incorporate 3H-thymidine into the new strands. Using a scintillation beta-counter the extent of cell<br />
division can be determined by measurement of radioactivity in DNA recovered from the cells (usually measured in counts per<br />
minute or cpm). Relative counts are compared to a control sample to determine the extent of cell <strong>proliferation</strong>.
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