Global Sequence Alignment: the Needleman-âWunsch Algorithm

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Why align sequences? Biological sequence similarity suggests funcLonal similarity and common evoluLonary origins (homology). Beta globin evoluLonary trajectory: Alignments quanLfy the similarity between different sequences within and between species. Ancetral globin: h[p://www.nature.com/scitable/topicpage/dna-‐deleLon-‐and-‐duplicaLon-‐and-‐the-‐associated-‐331
Why align sequences? Alignments can highlight funcLonally relevant regions of a DNA/protein sequence: FuncLonally important regions oben have be[er alignments due to evolu5onary conserva5on. adapted from: h[p://www.pnas.org/content/104/25/10388/suppl/DC1#F5
Page 1 and 2: Global Sequence Alignment: the
Page 3 and 4: What is the intuiLon behind s
Page 5: Why align sequences? • Alignme
Page 9 and 10: There are many possible alignme
Page 11 and 12: Which is the best alignment? s
Page 13 and 14: Idea: How can we find an opLm
Page 15 and 16: So what can we do?
Page 17 and 18: Some NotaLon • s 1 [1..i] = t
Page 19 and 20: The Key Insight • Think about
Page 21 and 22: Dynamic Programming Approach •
Page 23 and 24: Start with base condiLons D(i,j
Page 25 and 26: Now compute M(i,j) row by row.
Page 27 and 28: Now compute M(i,j) row by row.
Page 29 and 30: How do we construct an opLmal
Page 31 and 32: Is this algorithm feasible? •
Page 33 and 34: When would you want a local a

Global Sequence Alignment: the Needleman-âWunsch Algorithm

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Global Sequence Alignment: the Needleman-âWunsch Algorithm