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Leeds Flow Day 2011 Programme - Faculty of Biological Sciences

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“Go with <strong>Flow</strong> in <strong>Leeds</strong>”- <strong>Leeds</strong> Imaging and <strong>Flow</strong> Cytometry <strong>Day</strong><br />

<strong>Programme</strong><br />

09:00 Registration<br />

Medical Lecture Theatre, Level 7, Worsley Building, University <strong>of</strong> <strong>Leeds</strong><br />

Friday November 25 th <strong>2011</strong><br />

09:00-10:00 “<strong>Flow</strong>ing Breakfast” C<strong>of</strong>fee & Biscuits and Commercial Stall Viewing<br />

10:00-12:00 First Lecture Session<br />

10:00-10:10 Introduction<br />

Chair: Dr Graham Cook<br />

10:10-10:35 Talk 1 (LIMM <strong>Flow</strong> Cytometry Facility Presentations)<br />

Dr Yasser El-Sherbiny – “Differential NK Cell Killing Responses in Vitro using <strong>Flow</strong> Cytometry and<br />

Indo1 measurement <strong>of</strong> calcium flux <strong>of</strong> NK cells activation through NKG2D/CD16 cross linking.”<br />

10:35-10:55 Talk 2 (BD Biosciences)<br />

Dr Stephen Rackshaw – “New Applications in combined measurement <strong>of</strong> Proliferation & Apoptosis<br />

and protein screening using Lyoscreen and CBA.”<br />

10:55-11:20 Talk 3 (Institute for Molecular and Cellular Biology, <strong>Faculty</strong> <strong>of</strong> <strong>Biological</strong> <strong>Sciences</strong>,<br />

University <strong>of</strong> <strong>Leeds</strong>)<br />

Dr Nicola Stonehouse – Characterisation <strong>of</strong> RNA aptamers to human papilloma virus oncoproteins.<br />

11:20-11:40 Talk 4 (Biolegend)<br />

Dr Jane Limer – “Changes in Multicolour <strong>Flow</strong> Cytometry – The staining mix thickens.”<br />

12:00-13:00 “Priming the System” Buffet Luncheon and Stall Viewing<br />

Food & stalls provided by our sponsors<br />

Sponsors<br />

BD Biosciences<br />

Biolegend<br />

2BScientific<br />

Beckman Coulter<br />

Miltenyi<br />

Cambridge Biosciences<br />

VH Bio<br />

Sigma- Aldrich<br />

Amnis<br />

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13:00-14:30 Second Lecture Session<br />

13:00-13:10 Introduction<br />

Chair: Pr<strong>of</strong>. Eric Blair<br />

13:10-13:20 Talk 5 (Miltenyi)<br />

Jessica Moore – “Bead Pre-enrichment <strong>of</strong> rare cell subsets for analysis using flow cytometry.”<br />

13:30-14:00 Talk 6 (LIMM <strong>Flow</strong> Cytometry Facility Presentations)<br />

Dr Sally Boxall – “Isolation and characterisation <strong>of</strong> human multipotential stromal cells.”<br />

14:00-14:20 Talk 7 (Institute <strong>of</strong> Membrane and Systems Biology, <strong>Faculty</strong> <strong>of</strong> <strong>Biological</strong> <strong>Sciences</strong>,<br />

University <strong>of</strong> <strong>Leeds</strong>)<br />

Dr Mark Rakabowchuk – ‘Using FACS to enumerate endothelial progenitor cells and microparticles<br />

from humans and murine models’.<br />

14:20-14:40 Talk 8 (Amnis)<br />

Dr Mike Rooney – “The Imagestream X – A New Direction in <strong>Flow</strong> Cytmetry.”<br />

14:45-15:15 “Light Snack” C<strong>of</strong>fee & Biscuits and Commercial Stall Viewing<br />

15:15-16:30 Third Lecture Session<br />

15:15-15:35 Talk 9 (Beckman Coulter)<br />

Dr Ian Brotherick – Title TBA (Kaluza and its enhanced discriminative power for multiparameter<br />

analysis)<br />

15:35-16:00 Talk 10 (Institute for Molecular and Cellular Biology, <strong>Faculty</strong> <strong>of</strong> <strong>Biological</strong><br />

<strong>Sciences</strong>, University <strong>of</strong> <strong>Leeds</strong>)<br />

James Findlay – “<strong>Flow</strong> Cytometry - a tool for understanding the interactions between<br />

adenovirus and immune cells.”<br />

16:00-16:30 Talk 11 (LIMM <strong>Flow</strong> Cytometry Facility Presentations)<br />

Dr Frederique Ponchel – “T cell subset and moncyote monitoring in Rheumatoid patients<br />

undergoing ablative therapy using Multicolour <strong>Flow</strong> cytometry.”<br />

16:30-18:00 “System Shutdown” Wine & Cheese<br />

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