Protein Biotechnology

Content
1. Biochemical characteristics of proteins. Protein synthesis. The
overview of the differences between the eukaryotic and prokaryotic
protein synthesis ............................................................................ 10
2. Protein folding, 3D structure formation. Chaperons. Folding problems,
folding diseases. ............................................................................ 20
3. Protein sorting and targeting ....................................................... 33
4. The examination of protein structure: X-ray crystallography, NMR,
mass spectrometry ......................................................................... 39
5. Protein purification (chromatographic techniques) and analysis (SDS-
PAGE, 2DE, mass spectrometry). ...................................................... 46
6. The posttranslational modification of proteins and their analysis using
proteomics methods ....................................................................... 55
7. The study of protein-protein interactions ...................................... 61
8. Heterologous expression I .......................................................... 69
9. Heterologous expression II ......................................................... 73
10. Heterologous expression III ..................................................... 78
11. Protein engineering ................................................................. 84
12. Production of human therapeutic proteins .................................. 92
13. Production of human therapeutic enzymes ................................. 99
14. Production of diagnostic enzymes ............................................ 104
15. The application of therapeutic proteins. Possible administration,
perspectives and future possibilities. The national and international
requirements for protein therapeutic products .................................. 111