AN MicroPlate - Oxoid

AN MicroPlate
Anaerobe Identification Test Panel
A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12
N-Acetyl- N-Acetyl- N-Acetyl- Adonitol Amygdalin D-Arabitol Arbutin D-Cellobiose α-Cyclodextrin β-Cyclodextrin Dextrin
Water D-Galactosamine D-Glucosamine D-Mannosamine
B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12
Dulcitol I-Erythritol D-Fructose L-Fucose D-Galactose D-Galacturonic Gentiobiose D-Gluconic D-Glucosaminic α-D-Glucose Glucose-1- Glucose-6-
Acid Acid Acid Phosphate Phosphate
C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12
Glycerol D,L-α-Glycerol m-Inositol α-D-Lactose Lactulose Maltose Maltotriose D-Mannitol D-Mannose D-Melezitose D-Melibiose 3-Methyl-
Phosphate D-Glucose
D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12
α-Methyl- β-Methyl- α-Methyl- β-Methyl- Palatinose D-Raffinose L-Rhamnose Salicin D-Sorbitol Stachyose Sucrose D-Trehalose
D-Galactoside D-Galactoside D-Glucoside D-Glucoside
E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12
Turanose Acetic Acid Formic acid Fumaric Acid Glyoxylic Acid α- Hydroxy- β- Hydroxy- Itaconic Acid α-Ketobutyric α-Ketovaleric D,L-Lactic L-Lactic Acid
butyric Acid butyric Acid Acid Acid Acid
F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12
D-Lactic Acid D-Malic Acid L-Malic Acid Propionic Acid Pyruvic Acid Pyruvic Acid D-Saccharic Succinamic Succinic Acid Succinic Acid M-Tartaric Acid Urocanic Acid
Methyl Ester Methyl Ester Acid Acid Mono-Methyl
Ester
G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12
Alaninamide L-Alanine L-Alanyl- L-Alanyl- L-Alanyl- L-Asparagine L-Glutamic Acid L-Glutamine Glycyl- Glycyl- Glycyl- Glycyl-
L-Glutamine L-Histidine L-Threonine L-Aspartic Acid L-Glutamine L-Methionine L-Proline
H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12
L-Methionine L-Phenylalanine L-Serine L-Threonine L-Valine L-Valine plus 2'-Deoxy Inosine Thymidine Uridine Thymidine-5'- Uridine-5'-
L-Aspartic Acid Adenosine Mono-phosphate Mono-phosphate
INTRODUCTION
The Biolog AN MicroPlate (Figure 1) is designed for
identification of a very wide range of anaerobic bacteria,
including the genera Bifidobacterium, Clostridium,
Eubacterium, Fusobacterium, Lactobacillus, Lactococcus,
Megasphaera, Pectinatus, Pediococcus, Peptostreptococcus,
Propionibacterium and Weissella. These genera are important
in industrial and environmental applications, especially the
food industry where they are responsible for both food
production and food spoilage. Some clinical species are not
currently in our database. These will be added when they
meet our performance criteria. The AN MicroPlate employs
the same redox chemistry used in the Biolog GP2 and GN2
MicroPlates. This chemistry, based on reduction of
tetrazolium, responds to the process of metabolism (oxidation
of substrates) rather than to metabolic by-products (e.g. acid).
Biolog’s universal chemistry works with any carbon source
and greatly simplifies the testing process, as no color
developing chemicals need to be added after incubation.
AN MICROPLATE
The Anaerobe Database contains over 350 taxa of anaerobic
bacteria including over 70 species of lactic acid bacteria.
FIGURE 1. Carbon Source in AN MicroPlate
The Biolog AN MicroPlate performs 95 discrete tests
simultaneously and gives a characteristic reaction pattern
called a “metabolic fingerprint”. These fingerprint reaction
patterns provide a vast amount of information conveniently
contained on a single Biolog MicroPlate. The patterns are
compared using Biolog MicroLog database software to give
an identification.
Other anaerobic kit-based identification methods rely on fewer
tests to perform identifications. Therefore an anaerobic
organism that was previously characterized by another method
may yield an identification result that differs from the Biolog
AN MicroPlate & Database. This difference may be due to
several factors. When determining the validity of an
anaerobic identification result from the Biolog AN MicroPlate
and another method, consider the following:
o The MicroLog System bases its identification on a
larger number of tests
o The MicroLog System covers more species
o The taxonomy of many anaerobic genera is poorly
defined