Precis Rapid Culture Methods - Oxoid

See how easy the methods are to use
DEDICATED TO MICROBIOLOGY
Protocol for Listeria Precis Method
Day 0: Enrichment
25g or 25mL of sample + 225mL ONE Broth-Listeria
Incubate for 24 ± 2 hours at 30°C
Day 1: Plating
Using a 10µL microbiological loop inoculate
a single Brilliance Listeria plate.
Incubate for 22–26 hours at 37°C
(for meat samples
re-incubate plates that
show no blue colonies
with halos for a further
22–26 hours at 37°C)
Select green-blue
colonies with halos
for confirmation
Day 2: Results
If present, confirm blue/green colonies with halos as
L. monocytogenes using the O.B.I.S. mono test. Alternatively,
confirm using standard ISO methods.**
**If there is insufficient material to carry out an O.B.I.S. mono test, or if a mixed culture of
L. monocytogenes and other Listeria species is suspected, first purify suspect colonies by sub-culture
onto a second Brilliance Listeria plate.
With today’s demands for faster results to pathogen testing in food and environmental samples, Precis Listeria and Salmonella methods, give
your laboratory substantial time and labour savings compared to the traditional ISO methods. Validated by AFNOR to the ISO 16140 standard and
recently included in the M&S manual, these methods require just one broth and one plate, followed by confi rmation. With minimal
hands-on time, they provide results in just 2 days. To improve workfl ow and maximize your effi ciency, put your trust in the Precis methods.
For more information about these products, please visit www.oxoid.com
Oxoid and Remel are specialty microbiology brands of Thermo Fisher Scientifi c. Our products are available worldwide.
www.oxoid.com
Tel: +44 (0) 1256 841144
oxoid.info@thermofi sher.com
© 2011, Thermo Fisher Scientifi c Inc. All trademarks are the property of Thermo Fisher Scientifi c, Inc. and its subsidiaries.
Copyright to photos held separately. All rights reserved.
Protocol for Salmonella Precis Method
Day 0: Enrichment
25g or 25mL of sample + 225mL ONE Broth-Salmonella
Incubate for 16–20 hours at 42°C
Day 1: Plating
Using a 10µL microbiological loop inoculate a single
Brilliance Salmonella plate. Incubate for 22–26 hours at 37°C.
Brilliance Salmonella Agar incorporates novel Inhibigen
technology which improves Salmonella recovery by
reducing background flora.
Day 2: Results
If present, select a well isolated purple coloured colony and test
using the Oxoid Salmonella Latex Test. Alternatively, confirm
purple colonies using standard ISO methods.
Select purple colonies for confirmation
Part of Thermo Fisher Scientifi c
Folio No LT1460A/MS/08/11