polarAIDE⢠- TK Biotech
polarAIDE⢠- TK Biotech
polarAIDE⢠- TK Biotech
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polarAIDE<br />
Automatic assessment of oocyte quality and beyond<br />
OCTAX polarAIDE is the next generation integrated polarization microscopy system<br />
based on OCTAX ICSI Guard technology*.<br />
Take advantage of OCTAX´s modular high end technology for evaluating your oocytes:<br />
OCTAX polarAIDE TM is available as stand-alone system, as an upgrade to OCTAX ICSI Guard TM or as an<br />
add-on to OCTAX Laser Shot TM systems.<br />
All functions are handled by one easy-to-use software: OCTAX EyeWare TM is the unique platform for the<br />
control of all OCTAX products: laser, polarization microscopy, image processing, data storage and<br />
motorized stage.<br />
Main features:<br />
Quantification of the birefringence of the zona pellucida<br />
Automatic scoring of the developmental potential of oocytes in seconds<br />
Orientation-independent live imaging of the meiotic spindle<br />
Simultaneous view of oocyte morphology and spindle<br />
Self-calibrating, no active calibration required<br />
One button instant on/off function for convenient use<br />
Spindle imaging under normal light intensity (bright field)<br />
Full OCTAX Laser Shot TM and Fertilase ® compatibility, i.e. one camera, one software and one<br />
database for laser control, spindle visualization and image processing<br />
Support of all OCTAX EyeWare TM features (image acquisition and archival, video and time lapse<br />
video recording, biometric measurements etc.)<br />
Screenshots<br />
Metaphase II oocyte, polarAIDE TM in bright field optics.<br />
Metaphase II oocyte, polarAIDE TM active, combined<br />
mode using red/green processed image. Red area<br />
indicates high birefringence.<br />
Metaphase II oocyte, polarAIDE TM acitve, scoring<br />
mode. Pink area indicates high birefringence. Colorcoded<br />
numerical score for oocyte quality is displayed<br />
automatically.<br />
System Components:<br />
Circular polarizer and GIF filter<br />
Liquid crystal analysis slider<br />
High resolution OCTAX USB 2.0 digital camera<br />
OCTAX polarAIDE TM software<br />
polarAIDE TM is currently available for:<br />
Nikon TE2000, TE200/300, Diaphot 200/300<br />
Olympus IX 50/70 and IX 51/71<br />
Zeiss Axiovert 200, 135, 100<br />
Leica DMIRB, DMI 4000, DMI 6000<br />
other microscopes on request<br />
For operating polarAIDE, oocytes have to be placed in glass bottom dishes instead of plastic dishes.<br />
A heated stage is mandatory to maintain optimum temperature. PolarAIDE supports high resolution<br />
live video display (1280x1024 pixel) on selected computer hardware.<br />
*) Patents pending
polarAIDE<br />
Uncover the oocyte´s secrets at a glance!<br />
Automatic assessment of oocyte quality<br />
Automatic scoring of the developmental potential of oocytes is based on quantitative analysis of the<br />
birefringence properties of the inner layer of the zona pellucida. Scoring was validated in a prospective<br />
study by Montag et al. (2006). This fast and user-independent analysis at the oocyte stage may also be<br />
performed in countries with strict embryo protection laws.<br />
Transfer of embryos derived from oocytes with high birefringence of the zona pellucida resulted in<br />
significantly higher pregnancy rates compared to embryos with a low birefringence at the oocyte stage<br />
(Montag et al., 2006; Shen et al., 2005). The study of Montag et al. demonstrated that analyzing zona<br />
birefringence to identify the best zygotes resulted in significantly increased pregnancy rates as compared<br />
to conventional pronuclear scoring.<br />
Automatic scoring of metaphase II oocytes by polarAIDE TM . Zona is detected automatically by the measurement tool (green circles). Automatic analysis of birefringence properties of the zona<br />
pellucida is performed at 180 points. Pink area indicates high birefringence. Validated score (color-coded and numerical) indicating oocyte quality is displayed automatically.<br />
Left: oocyte with high quality score; right: oocyte with low quality score.<br />
Oocyte maturity evaluation<br />
Evaluate oocyte maturity after in vitro maturation, in low<br />
responder patients or before polar body biopsy and optimize<br />
the timing of ICSI or polar body biopsy. Only by using<br />
polarization microscopy, different meiotic stages can be<br />
clearly identified. PolarAIDE TM reveals to be telophase I what<br />
appeared to be metaphase II under brightfield optics (lower<br />
image). Performing ICSI on such oocytes is likely to result in<br />
abnormal fertilization. Polar body biopsy could even be lethal.<br />
Quality control<br />
The spindle is extremely sensitive to temperature and pH<br />
changes and will immediately indicate suboptimal culture<br />
conditions. High proportions of oocytes displaying spindles<br />
demonstrate optimal temperature and pH conditions in the<br />
dish placed on the microscope stage. Get the direct feedback<br />
from your oocytes!<br />
Montag M., Schimming T., Gassner P., van der Ven H. (2006)<br />
Oocyte zona birefringence intensity is associated with embryonic implantation potential.<br />
Hum. Reprod. 21(Suppl. 1):i67.<br />
Ordering information: MTG Medical Technology Vertriebs-GmbH, Opalstrasse 32, 84032 Altdorf, Germany<br />
Phone +49 (0) 871 975 19-0, Fax +49 (0) 871 975 19-70, mail@mtg-de.com<br />
OCTAX ICSI Guard TM and polarAIDE TM are currently not available in the U.S.A.
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