pGEM®-3Zf(+) Vector Technical Bulletin, TB086 - Gene Synthesis
pGEM®-3Zf(+) Vector Technical Bulletin, TB086 - Gene Synthesis
pGEM®-3Zf(+) Vector Technical Bulletin, TB086 - Gene Synthesis
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II.<br />
Product Components<br />
Product Size Cat.#<br />
pGEM ® -<strong>3Zf</strong>(+) <strong>Vector</strong> 20µg P2271<br />
The pGEM ® -<strong>3Zf</strong>(+) <strong>Vector</strong> is provided with a glycerol stock of bacterial strain JM109. The<br />
JM109 cells do not contain vector and are not competent cells.<br />
Storage Conditions: Store the pGEM ® -<strong>3Zf</strong>(+) <strong>Vector</strong> at –20°C and the glycerol<br />
stock of JM109 cells at –70°C.<br />
III.<br />
Multiple Cloning Site and Circle Map<br />
T7 Transcription Start<br />
5′ . . . TGTAA TACGA CTCAC TATAG GGCGA ATTCG AGCTC GGTAC CCGGG GATCC<br />
T7 Promoter<br />
EcoR I Sac I Kpn I Ava I<br />
Sma I<br />
BamH I<br />
SP6 Transcription Start<br />
TCTAG AGTCG ACCTG CAGGC ATGCA AGCTT GAGTA TTCTA TAGTG TCACC TAAAT . . . 3′<br />
Xba I<br />
Sal I<br />
Acc I<br />
Hinc II<br />
Pst I<br />
Sph I<br />
Hind III<br />
SP6 Promoter<br />
0283MA06/9A<br />
Figure 1. pGEM ® -<strong>3Zf</strong>(+) <strong>Vector</strong> promoter and multiple cloning site sequence. The<br />
sequence shown corresponds to RNA synthesized by T7 RNA polymerase and is complementary<br />
to RNA synthesized by SP6 RNA polymerase. The strand shown is complementary<br />
to the ssDNA strand produced by this vector.<br />
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Telephone 608-274-4330 · Fax 608-277-2516 · www.promega.com<br />
Part# <strong>TB086</strong><br />
Page 2<br />
Printed in USA.<br />
Revised 1/03
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