puramatrix in vivo delivery protocol with and without cells - 3DM Inc.

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PURAMATRIX TM
IN VIVO DELIVERY PROTOCOL WITHOUT CELLS
SUCROSE ADDITION FOR IN VIVO ADMINISTRATION:
In order to retain the osmotic balance of injected tissue, we recommend adding sucrose to the PuraMatrix
solution to achieve a final concentration of 10% sucrose.
1. Add 100 mg sucrose (Mallinckrodt, catalog #: MK772302) per 1 ml PuraMatrix solution (final
concentration of 10%).
2. Pipette up and down to mix and dissolve sucrose.
3. Load PuraMatrix sucrose mix into syringe. For syringe filling, we recommend a 20 G needle with a 1
ml syringe. Change needle to 30G and inject into tissue of interest.
DELIVERY OF PRE-GELLED PURAMATRIX AT PH 7.2:
PuraMatrix can also be pre-gelled in tubes for delivery in vivo. PuraMatrix gels at pH 4.5-5.0 or
greater. No further gellation will occur in vivo after injection and this procedure will result in the injection
of PuraMatrix gel fragments, hence a less cohesive bolus in vivo.
1. Aliquot 500 µl of PuraMatrix into a microtube.
2. Slowly add 1.0 ml PBS over the top of the PuraMatrix.
3. Let sit for 10-12 hours and change PBS. pH of gelled PuraMatrix will reach equilibrium within 24-48
hours.
3DM, INC • PO BOX 425025 • CAMBRIDGE, MASSACHUSETTS • 02445 USA
WWW.PURAMATRIX.COM • EMAIL: CONTACT@PURAMATRIX.COM • PHONE: 877.430.9595 • FAX: 877.430.9595