IDENTIFICATION OF COPPER-INDUCIBLE GENES IN Pistia stratiotes

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LIST <strong>OF</strong> ABBREVIATION ACP Annealing Control Primer Al Aluminium As Arsenic ATP Adenosine triphosphate BCP 1-Bromo-3-Chloropropane bp base pair BSA Bovine Serum Albumin Cd Cadmium cDNA Complementary DNA Cl Chloride Co Cobalt Cr Chromium Cu Copper DEG Differentially expressed gene DEPC Diethylpyrocarbonate DNA Deoxyribonucleic acid dNTP Deoxyribonucleoside triphosphate E. coli Escherichia coli EDTA Ethylene diaminetetraacetic acid Fe Ferum GSP Gene specific primer Hg Mercury HSP Heat shock protein xiii
HNO 3 Nitric acid IPTG Isopropyl-β-D-thiogalactopyranoside K Kalium/potassium kb Kilobase pair kPa kilo Pascal kDa Kilo Dalton LB Luria-Bertani Mg Magnesium MgCl 2 Magnesium chloride M-MLV-RT Molony murine leukemia virus reverse transcriptase Mn Mangan MOPS 3-[N-Mopholino]propanesulphonic acid mRNA Messenger RNA MT Metallothionein Na Natrium/sodium NaCl Natrium/Sodium chloride NCBI National Center of Biotechnology Information Ni Nickel OD Optical density OECD Organization for Economic Cooperation and Development Pb Plumbum/lead PCR Polymerase chain reaction PTMs Potentially toxic metals ppm part per million RLM-RACE RNA ligase-mediated and rapid amplification of 5’ cDNA end xiv
Page 1 and 2: IDENTIFICATION OF COPPER-INDUCIBLE
Page 3 and 4: ACKNOWLEDGEMENT First of all, I wou
Page 5 and 6: 2.8 The use of living organism to m
Page 7 and 8: 3.16.3.4 Reverse transcription of m
Page 9 and 10: LIST OF FIGURES 3.1 Vector used in
Page 11 and 12: LIST OF TABLES x Pages 2.1 The role
Page 13: LIST OF PLATES 2.1a Side view of Pi
Page 17 and 18: IDENTIFICATION OF COPPER-INDUCIBLE
Page 19 and 20: 1.0 Introduction For many years, th
Page 21 and 22: ioindicator characteristic after be
Page 23 and 24: and may identify potential diagnost
Page 25 and 26: Plate 2.1a. Side view of Pistia str
Page 27 and 28: 2.3 Effects of heavy metals on the
Page 29 and 30: decreasing red cell survival in car
Page 31 and 32: and efficient functioning of the bo
Page 33 and 34: The suggested safe level of copper
Page 35 and 36: uild-up of toxic concentrations at
Page 37 and 38: 2.7 Toxicity Test Toxicity test is
Page 39 and 40: 2.8.1 Bioindicator Adam (1990) defi
Page 41 and 42: This technique makes use of biologi

IDENTIFICATION OF COPPER-INDUCIBLE GENES IN Pistia stratiotes

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