IDENTIFICATION OF COPPER-INDUCIBLE GENES IN Pistia stratiotes

LIST OF FIGURES
3.1 Vector used in this study 33
3.2 Sequence of Genefishing DEG dT-ACP1 primer 38
3.3 Outline of RLM-RACE method 50
3.4 Structure of GeneRacer TM RNA Oligo 53
4.1 Copper content in the shoot and root of Pistia stratiotes 58
4.2 Total cellular RNA isolated from shoot of untreated and copper- 60
treated Pistia stratiotes
4.3 The PCR products of differentially expressed gene fragments 62
using the combination of dT-ACP primer with ACP2
4.4 The PCR products of differentially expressed gene fragments 62
using the combination of dT-ACP primer with ACP11
4.5 The purified PCR products 64
4.6 Nucleotide sequence of fragment 1 66
4.7 Nucleotide sequence of fragment 2 67
4.8 Nucleotide sequence of fragment 3 68
4.9 Amplification of copper-inducible candidate gene fragments 73
using gene specific primers
4.10 Dose response expression studies of (A) Total cellular RNA 75
isolated from shoot of un-treated and copper-treated Pistia
stratiotes (B) candidate gene fragment 1, (C) candidate
gene fragment 2, and (D) candidate gene fragment 3
4.11 Amplification of the 5’ end of the putative full length fragment 1 77
cDNA using RACE-PCR
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