department of microbial biotechnology - Bharati Vidyapeeth ...

December 22nd 2008Dr. M. K. Bhan,Secretary, DBTGovernment of Indiavisited the department2

Microbial biotechnology(PG) laboratoryPracticals conducted :DNA isolation frompro and eukaryotesRNA isolationAgarosegelelectrophoresisRestriction digestion,ligationCloningPCRSouthernhybridizationPrimer designing4

Research laboratoryProjects underway:Microbial production ofomega-3-fatty acid (Incollaboration withIRSHA, BVDU) fundedby DBT, Govt. of IndiaOther projects are relatedto:Marine biotechnologyProbioticsAntimicrobialcompounds frommedicinal plants5

2009: Fermentationandscale up facilityestablished6

3. Instrumental facilitiesMajorEquipment Make Cost (Rs.) Date of purchasePCR Eppendorf 620801 15/09/04Centrifuge Plastocrapt 488796 06/09/05Gel documentation B-Genei 472388 23/08/06Laminar air flow unit-03 Micro filt -02Envair-01239759 23/11/05,09/09/08Cooling Microfuge Remi 124763 14/11/06Electrophoresis powersupply-04B-Genei 117896 22/08/05,10/04/06Orbital shaking incubator Remi 103140 05/10/05Electronic balance-01 Mettler 84375 12/03/08Microscope binocular -02 Olympus CH20 80550 24/08/05Compound Microscopes – 5 Magnus 80265 08/09/09Trans-illuminator-02 B-Genei 78570 19/07/06Deep freeze (-400C) -01 Remi 66402 23/08/08Ligation bath-01 B-Genei 48094 01/10/05Hot air oven-02 Bio-Technics 44404 27/08/05Sony VPL EX-7 LCD projector Space office44000 28/08/09(Class room) -02system Pvt. Ltd.Incubators -03 Bio-Technics 42306 27/08/05Refrigerator -02 LG 37400 03/09/04Rota evaporator -01 Superfit 36110 15/05/07Distillation unit (Glass-01) Local, 28245 05/10/05Autoclave-01 Lab hosp 26530 04/01/05Autoclave -01From Hotel0 NAManagementWater bath -02 Bio-Technics 20429 14/09/05Colorimeter -02EquiptronicsSystronics19850 16/12/0804/09/04Balance-02 Tapson, Master 19800 27/08/05Water bath shaker-01 Neolab 17865 05/10/05Digital pH meter-01 Coslab 15946 08/09/09Total 2958684MinorMicro-oven -01 Lloyd 8505 08/09/09Digital pH meter-01 Global 8415 27/08/05Vortex mixer-03 Neolab -01,6589 23/09/05Fixa-pipette set (5 µl , 25 µl,50 µl, 100 µl, 500 µl & 1000µl)Variable pipette (10µl-02,20µl-01, 100µl-02, 200µl-01 &Remi -02Fixa-pipette &Bioera24/02/076048 08/09/09Pipette 23275 22/08/057

1000µl-01)Magnetic stirrer Spinit 4890 09/09/09Digital colony counter-01 Elite 4275 24/02/07Bench top cooler-02 (B-Genei) 3527 19/07/06Magnetic stirrer-01 Neolab 2848 23/09/05Mixer Phillips 2457 08/09/09Hot plate-01 Bio-Technics 1197 27/08/05Stopwatch-02 Racer 900 08/09/09Total 72926Project equipmentsDistillation unit (Steel-01) Local7834 18/02/09Lab EnterpriseTable top Centrifuge-01 Remi 16052 10/04/08Colorimeter -01 Equiptronics 9450 16/12/08Ultrasonicator Trans-o- sonic 90208 30/03/09Lyophilizer Biotechnologies 295481 30/03/09Fermentor Napro 1172712 31/03/09Total 1591737Grand total 46233478

4. AchivementsReviews submitted by T. Y. B. Sc. students:This activity was initiated for the T. Y. B.Sc. students to impart themskills of understanding the scientific subjects, comprehending relevantinformation in it and analytically summarizing the same in the form of abrief review.Sr.no. Title Author2005 - 20061 Flavonoids Kaveri Sidhu2 Bacterial pigments Mangesh S. Sawant3 Malaria, its cure and prevention Rajiv Choudhary4 Single cell protein Sarika Mane5Dengue virus : genome sequencing ,Abhinav Mishraprevention & cure6Signal peptides in prokaryotes : Literature Namrata Ghaytidakreview7 Carotenoids Tanya SoniGeneti variance exhibited in the IndianKartik Rajan8 population :its significance inpharmacogenomics9 Trichoderma as a bio Control agent Manish Kumar Pandey10 Proteases Aduja Naik11 Recombinant Interferon Veenita Shukla12 Bio preservatives Manpreet Grewal13 Hepatitis-C Jayant Pawar14 DNA-fingerprinting Swapanaja Patil15 Literatiure review osteoporosis Shewta Padhye2006-20071 Recombinant Vaccines Deepali Singh2 Detailed study of AIDS Amol Laddha3 Biofertilizers Pradnya Nikam4 Alternative therapy Jyoti Dipty5 Bio Fertilizers & Bio Pestisides Anup Shah6Synthesis of commercial recombinantSushmita Chawdhurymicrobes2007-20081 Food and drug administration Leena Hedge2 Bio Fertilizer-Azotobacter Bhagyashree Rajapure3The study of gourds with special reference toashgourds, bottle gourds, bitter gourds, ridgegourds, snake gourds.Vandita D’souza9

4Value of waste from brightly colouredSonam S. Dalimbakarvegetables5 Phytase : transforming a curse into a boon Rohan Kapoor6 Flavonoids : Grape skin Priyanka Tiwari7Biodegradation of polytheyene byAnkita Singhmicroorganisms8 Diabetes: Its herbal remedies Sarita Kumari9 Acacia concinna Neha Jaiswal10 Xenobiotics: Recalcitrant compounds Bahul Gidde11 Wine making Sagar Mahind12 Wine & human health Suman Ranjan Jha13 Rhizobium as a biofertilizer Twisha Singh2008-20091 Current status of hepatitis C vaccine Debanjana Dutta2 Steroids and their effect on human health Deepshikha Saraswat3 Probiotics: Immunomodulatory effects Arti Kateria4 Probiotics Amar Patil5 Biological databases Neel Shah6 Recovery of PUFA Shweta Arora7 Types of pollution in Antartica region Tolnur Malkarjappe8 Herbs as skin treatment Monica Athwani9Biodegradation of polyethylene byParinita Jhawarmicroorganisms2009-20101 Trichoderma viride: Its cultivation andAanchal KatochApplications2 Pathogens: Sources, Incidents, Fatalities and Ankita NarangRemedies3 Transformation of Penicillin in to APA -SignificanceVijaya LakshmiM. Sc. projects completedSr. no. Title of project Name of student2006 - 20071. Bio-diesel Deshmukh Siddharth2. A review on Spirulina platensis Kadam Deepa3. Citric acid production by AspergillusnigerSagolsem Bidhanchandra4.A) BiofuelsB) Extraction of lipids from marinemicroorganismsShaikh Anjuman5. Enzymes in food industriesAntibacterial activity of TinosporacordifoliaSoregaonkar Samir10

2007-20081.Extraction of polyunsaturated fattyacids from microbial sourcesAgarwal Aditi, Manjulatha2. RAPD analysis in Withania somnifera Jadhav Vipra3.Extraction, isolation and identificationof active ingredient from Abutilonpannosum and Canthium parviflorumand testing theirPhatan Ejaj4.Comparitive studies on probioticorganismsDixit Gauri5.Isolation & identification of salttolerant nitrogen fixingJadhav Girish Gmicroorganisms2008-20091.Screening & characterization ofHoda Sadal Aayadi,bacteria with fibrinolytic activity fromMaryam Ranjpourmarine source2.Antifungal activity of Murraya kurrayakoenigiiMajumder Debamita3.Production of omega-3 fatty acids frommarine microbial sourcesNirmal Pallavi4.DNA profiling & antimicrobial activity Pawar Joyti & Anugadeof medicinal plantsSheela5.Production of omega-3 fatty acids bymicro- organisms & optimization ofTiwari Nehacultural conditions6.Isolation & characterization of marinemicroorganism producing polysaturatedNikam Kapilfatty acid2009-20101. Screening of marine microorganismsRachanafor protease and lipase enzymes2. Elisha3.Ratna ganguli4.Screening of bioactive compounds fromSonal Majrekar5.marine microbial sourcesShruti Shree11

Projects completed by the students other than RGITBT:Sr. no. Student name Theme of research YearM.Sc. Microbiology (Y. M. College, Pune)1. Rohit Ajit Alasandkar Antimircobial activityof Tinospora cordifoliaand isolation of itsactive principles2007-082. H Abhishek Nair Study of lipidproduction byRhodotorula rubra.2007-083. Shantinath Khot Antimicrobial activity 2007-08of Nothapodytesfoetida andphytochemical studies4. Snehal Patil Screening of PUFA 2008-09producingmicroorganisms fromIndian Ocean5. Sandip Mulik Optimization of2008-09parameters for PUFAproduction in marinemicroorganisms fromIndian Ocean.B.E. Chemical Engineering1. Vishal Taneja Studies on culture 2007-082. Sunny Aroraconditions ofRhodotorula rubra forbiomass production2007-085. Research profilePh. D. programSr. No. Name ofthestudent1. Vipra V.Jadhav2. Dipti S.SalunkheTopic of researchBiotech potential ofmarine organismsand yeasts for PUFAStudies onpolyunsaturatedRegistrationyear20082008GuideDr. Mrs.RamaBhadekar12

fatty acids frommarinemicroorganisms andoleaginous yeastsPapers presentedR. Bhadekar, N. Meti, V. Jadhav, G. Patil and D.P.Nerkar In vitro cultivation andRAPD analysis in Withania somnifera Paper presented at Global AyurvedConference organized by Bharati Vidyapeeth University , Pune, between January 9-11, 2009.BR Ehsan & N K Bipinraj, Antimicrobial activity of the ethanolic extract of B.Laciniosa leaf, stem, fruit and seed. Afr. J Biotechnol., Global Ayurved Conferenceand Health Expo , 2009.Posters presented (International conferences)Ejaj Pathan, D. P. Nerkar and R. K. Bhadekar. Studies on phytochemicals fromAbutilon pannosum, (Kasali). Poster presented at Indo-Swedish InternationalConference on “Biotechnology for Sustainable Development” organized at NCL,Pune between Jan. 7-9, 2008.R. Bhadekar , N. Meti, M. Falaki Khalilsaraie, R. Alasandkar, and D. P. Nerkar,Antimicrobial activity of stem, leaf and callus induced from leaves of Tinosporacordifolia. Poster presented at Global Ayurved Conference organized by BharatiVidyapeeth University , Pune, between January 9-11, 2009.Pallavi Nirmal, Vipra Jadhav, D. P. Nerkar and R. K. Bhadekar. Poster presented at50th International Conference of Association of Microbiologists of India held atNCL, Pune, between Dec. 15 -18, 2009Laboratory manualsRama Bhadekar, Deepti Samarth and Dipti Salunkhe. Laboratory manual ofMicrobiology for M.Sc. Biotechnology (Sem. I and II), Rajiv Gandhi Institute of I.Tand Biotechnology, 2010.Rama Bhadekar, Vipra Jadhav and Deepti Samarth. Laboratory manual of GeneticEngineering for M.Sc. Biotechnology (Sem. III and IV), Rajiv Gandhi Institute of I.Tand Biotechnology, 2010.13

Recent PublicationsDipti Salunkhe, Neha Tiwari, Sandeep Walujkar, Rama Bhadekar. (2010) Halomonassp. Nov., an EPA producing mesophilic marine isolate from the Indian Ocean. PolishJournal of Microbiology (In press)Vipra Vijay Jadhav, Manoj Mahadev Jamle, Pravin Dnyaneshwar Pawar, MayurNimbadas Devare, Rama Kaustubh Bhadekar. Fatty acid profiles of PUFA producingAntarctic bacteria: correlation with RAPD analysis. Annals of Microbiology (InPress) DOI: 10.1007/s13213-010-0114-4.GirishYearAmountGajananJadhav,DiptiSambhaji Salunkhe, Devdas Punjaji Nerkar, Rama Kaustubh Bhadekar (2010)Isolation and characterization of salt-tolerant nitrogen-fixing microorganisms fromfood. EurAsian Journal of Biosciences, 4, 33 – 40.Girish Gajanan Jadhav, Dipti Sambhaji Salunkhe, Devdas Punjaji Nerkar, RamaKaustubh Bhadekar (2010) Novel Staphylococcus sp isolated from wall scrapings ofhistorical building in India. Annals of Microbiology, 60, 197 – 201.BR Ehsan, V Awad & N K Bipinraj, Antimicrobial activity of the ethanolic extract ofB. Laciniosa leaf, stem, fruit and seed. Afr. J Biotechnol., 8 (2009) 3565-3567.E A Singh, SY Kamble and N K Bipinraj, Medicinal plants from newly reporteddevraies (sacred groves) in Pune district. Ind. J Trad. Knowledge(in press).A Z Abubaker, B R Ehsan and N K Bipinraj, Antimicrobial activity of six Indianspices, ICFAI J.biotechnol (in press).S Mukherjee, N M pawar, S Thopte, B Nagarkar, A D Bhujbal, R Chowdhury, PRaina, N K Bipinraj & P K Pawar, Evaluation of anti- microbial potential ofVayasthapana rasayana formulation on some pathogenic bacteria, J Toxicol HerbalMed (in press).Grants receivedOngoing project on “Microbial production of Omega-3 fatty acid” in collaborationwith IRSHA funded by DBT, Govt. of India.14

2008 – 09 23. 32 Lakhs2009 – 10 3.0 Lakhs2010 - 11 4.82 Lakhs(awaited)Rs. 2 Lakhs for Molecular characterization of Indian strawberry cultivars, In-houseproject6. Executive summarySr. no. Category Accomplishments1. No. of publications2. T.Y. B.Sc. Reviews guided3.No. of M.Sc. students guided forprojects from 20064. No. of Ph. D. students4. Papers / Posters presented5. Conferences/Workshops attended14504323 Papers,3 Posters9 conferences,8Workshops1. IRSHA, BVDU, Pune6. Collaborations2. NCCS, Pune3. COEP, Pune7. Grants received28 Lakhs15

7. Library profileCollection of library books until 2009-10DepartmentBooks(2008-09)Books added(2009-10)Total No. ofBooksMicrobial Biotechnology 356 22 378Subscribed Journals & Magazines2009-2010Sr. No.Journals1 Current Science2 The ICFAI Journal of Life sciences3 The ICFAI Journal of Science & Technology5 The ICFAI Journal of Biotechnology6 The ICFAI Journal of Environmental Sciences7 The ICFAI Journal of Genetics & Evolution8 Journal of Biosciences9 Bio Research International10 Journal of Scientific and Industrial Research11 Indian Journal of Experimental Biology12 Research Journal of Biotechnology14 Indian Journal of Biochemistry & Biophysics15 Indian Journal of Biotechnology16 Trends in BiotechMagazines1 Scientific American (India)3 Chemical Weekly4 Bio-spectrum5 Down to Earth7 Proteomics8 Medicinal and Aromatic Plants Abstracts16