CURRICULUM VITAE FREY Joachim Prof. Dr. Institute for ...
CURRICULUM VITAE FREY Joachim Prof. Dr. Institute for ...
CURRICULUM VITAE FREY Joachim Prof. Dr. Institute for ...
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<strong>CURRICULUM</strong> <strong>VITAE</strong><strong>FREY</strong> <strong>Joachim</strong> <strong>Prof</strong>. <strong>Dr</strong>.<strong>Institute</strong> <strong>for</strong> Veterinary Bacteriology Date of birth: July 21 st 1951University of BernePlace of birth: ZürichLänggasstr. 122Nationality: SwissCH-3012 Bern, SwitzerlandMarital status: Married, 2 children: +41 31-631 2414 <strong>Prof</strong>ession: Molecular Biologist & BiochemistFAX: +41 31-631 2634Private: Schreinerweg 9CH-3012 Bern +41 31-305 6619Current PositionUNIVERSITY OF BERN, INSTITUTE FOR VETERINARY BACTERIOLOGY:<strong>Prof</strong>essorChair of the Department of Infectious Diseases, Vetsuisse Faculty, University of BernDirector of the <strong>Institute</strong> <strong>for</strong> Veterinary Bacteriology with 43 collaborators (since June 2000),Head of the Research Unit of the <strong>Institute</strong> of Veterinary Bacteriology, University of Berne, directing a researchgroup of 15 persons.Current ResearchMolecular mechanisms of bacterial virulence, Infection and inflammation; Microbiology; Genetics andBiochemistry; Vaccine development; Gene array technologies; Bacterial protein toxins, Adhesins andimmunogenic factors; Bacteria-host interactions in pathogenesis; Biological safety aspects of natural andgenetically modified organisms.The research topics involve:a) Biochemical and genetic study of virulence factors of Actinobacillus pleuropneumoniae. Purification of majortoxins, cloning, toxicity on polymorph nuclear neutrophils and macrophages, construction of derivative strainsdeleted <strong>for</strong> their virulence genes, protective antigens, elaboration of concepts <strong>for</strong> live vaccines.b) Mycoplasma mycoides subsp. mycoides SC: Adhesion, mechanisms of virulence, specific antigens. Impact ofmetabolic pathway products in virulence.c) Genetic and antigenic investigations of Mycoplasma conjunctivae, the etiologic agent of infectious keratoconjunctivitis IKC. New genetic and immunological detection methods.d) Virulence factors and epidemiological markers of ruminants’ Mycoplasmas.e) Type III secretion systems and effector proteins of inflammatory processes in Aeromonas salmonicida andother fish pathogenic Aeromonas speciesf) Clostridium chauvoei and Clostridium septicum: analysis of major virulence factors.g) Gene probes <strong>for</strong> the detection of bacterial virulence factors (Bio-safety assessment).h) Recombinant vaccine against botulism in horses.i) Molecular typing of Bacillus anthracis and Francisella tularensisj) Detection and genetics of Brachyspira sp.Previous Positions(1987-2000) UNIVERSITY OF BERN, INSTITUTE FOR VETERINARY BACTERIOLOGY:<strong>Prof</strong>essor (since 1996)Head of the Research Unit of the <strong>Institute</strong> of Veterinary Bacteriology, University of Berne(1981-1987) UNIVERSITY OF GENEVA, DEPARTMENT OF BIOCHEMISTRY:Maître assistant.(1984) UNIVERSITE DU QUEBEC A MONTREAL. CANADA, DEPARTEMENT DE BIOLOGIEInvited professor(1980-1981) MAX PLANCK INSTITUT, BERLIN, GERMANY, DEPARTMENT OF GENETICSResearch associate in the Group of <strong>Prof</strong>. K.N Timmis.(1975-1980) UNIVERSITY OF GENEVA, DEPARTMENT OF MOLECULAR BIOLOGY:
AssistantHonours particular functions & education(2009 - ) Board member, Scientific advisory board, Monash University, Clayton Melbourne, Australia, ARC Centreof Excellence in Structural & Functional Microbial Genomics(2004 – 2007) Dean, VETSUISSE FACULTY, UNIVERSITY OF BERN, SWITZERLAND(1992) UNIVERSITY OF BERN, FACULTY OF VETERINARY MEDICINEHabilitation, July 1992Habilitation thesis: Biochemical, genetic and immunological analysis of the haemolysins/cytolysins ofActinobacillus pleuropneumoniae.(1976-1980) UNIVERSITY OF GENEVA, DEPARTMENT OF MOLECULAR BIOLOGYPhD. (Sept. 1980)The role of the dnaA gene in the control of replication of chromosomal and plasmid DNA. Advisor <strong>Prof</strong>. L. Caro(1975-1976) UNIVERSITY OF GENEVA, DEPARTMENT OF MOLECULAR BIOLOGYCertificate of Specialisation in Molecular Biology (Post Graduate degree)Isolation and characterisation of small plasmids derived from R-factor R1.19, R6.5 and R100. <strong>Prof</strong>. L. Caro(1973-1975) UNIVERSITY OF UPPSALA, SWEDEN, DEPARTMENT OF BIOCHEMISTRYDiploma in Chemistry and Biochemistry (Fil. Kand.)Research: Purification and characterization of human thyroidea stimulating hormone and precursor of growthhormone from human pituitary glands. Advisor <strong>Prof</strong>. P. Roos.(1971-1973) UNIVERSITY OF GENEVA, FACULTY OF SCIENCE.First two years studies (propedeutics) <strong>for</strong> the diploma of chemistry and biochemistry.LanguagesGerman:English:French:Swedish:mother tonguefluentfluentgoodNominations, grants, awards, and competitive stipends, editorial functions2010 - Chair, International Organization of Mycoplasmology2009 - Scientific Board Member, Ctr. of excellence <strong>for</strong> structural and functional microbial genomics,MONASH University, Melbourne, Australia2003 - Member of the Swiss Federal Commission <strong>for</strong> Biological Safety, EFBS1998 - Member of the Scientific Committee of CNRS (Centre National de la RechercheScientifique), France.1998 - FAO-OIE-AU/IBAR-IAEA Expert on Contagious Bovine Pleuropneumonia in Africa1997 - Member of the International Committee on Systematic Bacteriology (ICSB),Subcommittee on the Taxonomy of Mollicutes1995 - 2000 President, COST Action 826 ”ruminants’ mycoplasmoses”, European Commission1995 Adjunct member, <strong>Institute</strong> of Molecular Agrobiology, The National University of Singapore.1995 – 2001 Member of the Swiss Federal Commission <strong>for</strong> Biological Safety, EFBS1994 Adolf Eduard Stein Prize, University of Berne.1989 - 1995 Member of the Commission <strong>for</strong> Biotechnological Safety in Research and Technology of theSwiss Academy of SciencesVeterinary Research, Editor, since 1998Veterinary Microbiology, Editor, since 1999Animal Health Research Reviews, since 2000Current and <strong>for</strong>mer grants2011-14 Swiss National Science Foundation: Patatin-like phospholipases are new putative major virulenceeffectors of the Aeromonas salmonicida subsp. salmonicida type-three secretion system. A model ofpathogenicity of Aeromonas and other pathogens. #31003A_135808/1 (CHF 375’000.--).CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 2/28
2007-10 Swiss National Science Foundation NRP 59 “Benefits and Risks of the Deliberate Release ofGenetically Modified Plants # 405940-115557 / 1 Collaborative Project with <strong>Dr</strong>. Michel Goldschmidt-Clermont, Université de Genève, (CHF 401’560.--).2005-10 Wellcome Trust UK: Development and Validation of new technologies <strong>for</strong> control of contagiousbovine pleuropneumonia (CBPP) in East Africa. # 075804/A/04/Z(4 years CHF 788'522.00; total Grant UK£ 1'500'000.00)2007-10 Swiss National Science Foundation: Type III secretion system in Aeromonas salmonicida.(3 years CHF 296’000.00)2006-09 KTI, Bundesamt für Berufsbildung und Technologie BBT Nr.7958.1 LSPP-LS(3 years CHF 199'800.00)2006-08 Paul Storm Grant, Intervet Intn. NL: Virulence attributes of Clostridium chauvoei.(2 years CHF 202’617.00)2006-08 KTI, Bundesamt für Berufsbildung und Technologie BBT Nr. 7304.1LSPP-LS:Construction ofrecombinant bacterial vaccine strains expressing colonization factors of enterotoxigenic Escherichiacoli (ETEC)(2 years CHF 125'233.00)2004-2007 Swiss National Science Foundation: Type III secretion system in Aeromonas salmonicida. (3y)2002-2004 Bundesamt für Veterinärwesen; Serologie der Schweine pleuropneumonie2003 AKZO-NOBEL, Intervet International BV Boxmeer, NL. Biotechnology; Grant2001-2003 Bundesamt für Veterinärwesen; Analyse der antigenen Proteine von Mycoplasma2001-2005 EU 5 th Framework. INCO, Development of improved vaccines against (CBPP)1997-2000 Swiss National Science Foundation Biotechnology Programme #5002-0450271996-2000 Swiss Ministry of Education and Science, BBW, Mycoplasmas Research C96.00731995-2000 COST action 826 Ruminant's mycoplasmoses (5 years)1995 AKZO-NOBEL, Intervet International BV Boxmeer, NL. Biotechnology Grant (1y)1994-1997 Swiss National Science Foundation Biotechnology Programme #5002-038920 (3y)1994 AKZO Intervet International BV Boxmeer, NL. Biotechnology Grant (1y)1993-1995 Stiftung 3M Nachweis von Clostridien-Toxinen (J. Nicolet, J. Frey)1992 Bundesamt für Veterinärwesen (C.jejuni) (J. Nicolet, J.Frey, A.Burnens)1992 AKZO Intervet International BV Boxmeer, NL. Biotechnology Grant (1y)1990-1993 Swiss National Science Foundation # 31-28401.90 (3 y)1990 AKZO Intervet International BV Boxmeer, NL. Biotechnology Grant (1y)1987-1990 Swiss National Science Foundation # 3.570.087 (3 y)1987-1990 Swiss National Science Foundation # 3.627.087 (3 y)1986 EMBO short term fellowship (University of Umeå).19841987 Grant of the Swiss National Foundation # 3.594.084 (3 y)1984 EMBO short term fellowship (University of Umeå).1980-1981 Junior Research Fellowship of the Swiss National Foundation (M.P.I. Berlin).Supervisor of Habilitations PhD, MD and DVM thesis (only from 2006 - present)Mavrot F. (2012) Infectious keratoconjunctivitis in free-ranging Alpine chamois and ibex: epidemiological,etiological and immunological investigations. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Scherer Alexandre (2011) Effect of agricultural use of streptomycin on the bacterial flora of animals. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Bürki Sibylle (2011) Real-time PCR-based diagnosis of Brachyspira hyodysenteriae and Brachyspira pilosicoliinfections in pigs. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Kaupp Gabriela (2011) Assessment of the humoral immune response to Clostridium sordellii toxin TcsL in horseswith equine atypical myopathy. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Sieber Sandra (2010) Evolution of horse infection and personnel colonization with multidrug-resistantStaphylococcus aureus in an equine clinic between 2005 and 2010. Inaugural-Dissertation der Vetsuisse-Fakultätder Universität Bern.Hinić Vladimira (2010) Differentiation and virulence of Brucella species. PhD Thesis, Graduate School, Universityof Bern.Unger Lucia (2010) Lethal toxin of Clostridium sordellii is associated with fatal equine atypical myopathy.Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 3/28
Balandytè Lina (2010) MLVA typing of Listeria monocytogenes strains isolated from brain samples of sheep, goatand cattle and comparison with strains of human food and environmental origin. Inaugural-Dissertation derVetsuisse-Fakultät der Universität Bern.Küng Elinae (2010) Identification and characterization of a novel RTX like leukotoxin in Avibacteriumparagallinarum. Master of Science, Naturwissenschaftlich-Philosophische Fakultät, Universität Bern.Panchaud Yann Philipp (2009) Bacterial infections in horses: A restrospective Study at the Unviersty EquineClinic bern (2004-2008), Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Goldschmidt-Clermont Elinor (2009) Virulence and control of the perch pathogen Aeromonas sobria. PhD Thesis,Graduate School, University of Bern.Rossetti Bigna Catrina (2009) Direct detection of Mycoplasma bovis in milk and tissue samples by real-time PCR.Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Stahl Maria Christina (2009) Immune response of horses to vaccination with the recombinant Hc domain ofBotulinum Neurotoxin types C and D. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Schnider Andrea (2009) Prevalence of Campylobacter jejuni and Campylobacter coli on Swiss broiler carcassesdetermined by comparative real-time PCR. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Wirz Simone Elisabeth (2009) Genotypes and antibiotic resistance of Campylobacter isolates from Swiss broilerduring 2008. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Perrin Magali (2009) Francisella spp. In ticks. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Gobeli Stefanie (2008) Analysis of a novel Pseudomonas chlororaphis subspecies as a potential probiotic againstAeromonas sobria infections in perch (Perca fluviatilis L.). Inaugural-Dissertation der Vetsuisse-Fakultät derUniversität Bern.Goy Naomi Daphné (2008) Quantification of Lawsonia intracellularis in porcine faecal samples by real-time PCRwith application in field samples. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Zurfluh Monika (2008) Typing of Campylobacter spp. Inaugural-Dissertation der Vetsuisse-Fakultät derUniversität Bern.Smole Ines (2008) Repression of common bull sperm flora and in vitro impairment of sperm motility withPseudomonas aeruginosa introduced by contaminated lubricant. Inaugural-Dissertation der Vetsuisse-Fakultätder Universität Bern.Dousse Florence (2008) Phenotypic identification of bacterial species of the family Pasteurellaceae isolated fromanimals. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Descluox Sibyll Thérèse (2007) Antibiotic resistance in Staphylococcus spp from dogs and cats. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Schnellmann Christina Judith (2007) antibiotic resistance in Staphylococcus spp. isolated from the skin of horsesfe<strong>for</strong> and after admission to clinic: Presence of new mecA and mph(C) variants.Hinic Vladimira (2007) Real-time PCR assay <strong>for</strong> the detection of Brucella spp in naturally infected wild boars.Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Walter Carole (2007) Molecular characterization of antibiotic resistant bacteria from bovine milk. Inaugural-Dissertation der Vetsuisse-Fakultät der Universität Bern.Wollschläger Nicole (2007) Diagnostik und Sanierung der hämorrhagisch nekrotisierenden Enteritis derSaugferkel (Clostridium perfringens Typ C) in der schweizerischen Schweine-population. Inaugural-Dissertationder Vetsuisse-Fakultät der Universität Bern.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 4/28
Bischof Daniela (2007) Genetic variations and virulence of the bovine pathogen Mycoplasma mycoides supsp.mycoides small colony type. Inaugural-Dissertation der Philosophisch-naturwissenschaftlichen Fakultät derUniversität Bern.Goldschmid-Clermont, Elinor (2007) Identification of bacterial isolates from the normal flora of perch (Percafluviatilis L.) and their inhibitory potential towards bacterial fish pathogens, including Aeromonas sobria. ThèseInaugurale de la Faculté Vetsuisse, Univesité de Berne.Schnell Barbara (2007) Bacteria-Amoebae interactions of pathogenic and non-pathogenic Aeromonas species.MSc, Universität Bern.Perreten Vincent (2006) Resistance in the food chain and in bacteria from animals: Relevance to humaninfections. Habilitationsschrift der Veterinär-Medizinischen Fakultät der Universität Bern.Ballmer Karin (2006) Genetic serotyping of Escherichia coli using DNA microarrays. Inaugural-Dissertation derVetsuisse-Fakultät der Universität Bern.Zimmermann Liza (2006) Molecular epidemiology and adhesion factors of Mycoplasma conjunctivae, theetiological agent of infectious kerato-conjunctivitis (Gemsblindheit). Inaugural-Dissertation der PhilosophischnaturwissenschaftlichenFakultät der Universität Bern.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 5/28
Publications <strong>Joachim</strong> FreyPeer reviewed articlesChandler, M., Silver, L., Frey, J. and Caro, L. (1977). Suppression of an Escherichia coli dnaA mutation by theintegrated R factor R100.1: Generation of small plasmids after integration. J. Bacteriol. 130: 303-311.Frey, J., Chandler, M. and Caro, L. (1979). The effects of an Escherichia coli dnaAts mutation on the replicationof the plasmids ColEl, pSC101, R100.1 and RTF-Tc. Molec. Gen. Genet. 174: 117-126.Frey, J., Chandler, M. and Caro, L. (1981). The initiation of chromosome replication in a dnaAts46 and a dnaA +strain at various temperatures. Molec. Gen. Genet. 182: 364-366.Bagdasarian, M., Lurz, B.R., Rückert, G., Franklin, F.C.H., Bagdasarian, M.M., Frey, J. and Timmis, K.N. (1981).Specific purpose plasmid cloning vectors, and a host vector system <strong>for</strong> gene cloning in Pseudomonas. Gene 16:237-247.Frey, J., Bagdasarian, M., Feiss, D., Franklin, F.C.H. and Deshusses, J. (1983). Stable cosmid vectors thatenable the introduction of cloned fragments into a wide range of Gram-negative bacteria. Gene 24: 299-308.Brady, G., Frey, J., Danbara, H. and Timmis, K.N. (1983). Replication control mutations of plasmid R6-5 andtheir effects on interactions of the RNA-I control element with its target. J. Bacteriol. 154: 429-436.Frey, J., Chandler, M. and Caro, L. (1984). Overinitiation of chromosome and plasmid replication in a dnaAcosmutant of Escherichia coli K12: Evidence <strong>for</strong> DnaA-DnaB interactions. J. Mol. Biol. 179: 171-183.Frey, J., Franklin, F.C.H. and Deshusses, J. (1985). Cloning of the catechol 2,3-oxygenase gene using high- andlow-copy number expression vectors. Biochemical Educ. 13: 27-31.Gauchat-Feiss, D., Frey, J., Belet, M. and Deshusses, J. (1985). Cloning of the genes involved in the myoinositoltransport in a Pseudomonas species. J. Bacteriol. 162: 324-327.Frey, J. and Timmis, K.N. (1985). ColD-derived cloning vectors that autoamplify in the stationary phase ofgrowth. Gene 35: 103-111.Frey, J. and Krisch, H.M. (1985). Omega mutagenesis in Gram-negative bacteria: a selectable DNA fragmentwhich is strongly polar in a wide range of bacterial species. Gene 36: 143-150.Haring, V., Scholz, P., Scherzinger, E., Frey, J., Derbyshire, K., Hatfull, G., Willets, N.S., and Bagdasarian, M.(1985). Protein RepC is involved in copy number control of the broad host range plasmid RSF1010. Proc. Natl.Acad. Sci. U.S.A. 82: 6090-6094.Frey, J., Ghersa, P., Palacios, P.G. and Belet, M. (1986). A physical and genetic analysis of the ColD plasmid. J.Bacteriol. 166: 15-19.Frey, J. and Belet, M. (1986). Mobilizable Cloning Vectors Derived from ColD: Their Use in Erwinia. Symbiosis 2:331-335.Fellay, R., Frey, J. and Krisch, H. (1987). Interposon Mutagenesis of soil and water bacteria: a family of DNAfragments designed <strong>for</strong> in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154.Frey, J., Mudd, E.A., and Krisch, H.M. (1988). A bacteriophage T4 expression cassette that functions efficientlyin a wide range of Gram-negative bacteria. Gene 62: 237-247.Piffaretti, J.-Cl., Arini, A. and Frey, J. (1988). pUB307 mobilizes resistance plasmids from Escherichia coli intoNeisseria gonorrhoeae. Mol. Gen. Genet. 212: 215-218.Ghersa, P., Lurz, R., Dobrinski, B., Deshusses, J., Belet, M., and Frey, J. (1988). Genetic evidence <strong>for</strong> theexistence of a repressor that modulates colicin D expression on plasmid ColD-CA23. Mol. Gen. Genet. 213: 36-41.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 6/28
Frey, J. and Nicolet, J. (1988). Purification and partial characterization of a hemolysin produced by Actinobacilluspleuropneumoniae type strain 4074. FEMS Micobiol. Lett. 55: 41-46.Frey, J. and Nicolet, J. (1988) Regulation of hemolysin expression in Actinobacillus pleuropneumoniae Serotype1 by Ca ++ . Infect. Immun. 56: 2570-2575.Fellay, R., Krisch, H.M., Prentki, P. and Frey, J. (1989). Omegon-Km: a transposable element designed <strong>for</strong> invivo insertional mutagenesis and cloning of genes in Gram-negative bacteria. Gene 76: 215-226.Frey, J., Perrin, J., and Nicolet, J. (1989). Cloning and expression of a cohemolysin, the CAMP factor ofActinobacillus pleuropneumoniae. Infect. Immun. 57: 2050-2056.Gygi, D., Nicolet, J., Frey, J., Cross, M., Koronakis, V., and Hughes, C. (1990) Isolation and expression of theActinobacillus pleuropneumoniae hemolysin gene; activation and secretion of the prohemolysin by the HlyC, HlyBand HlyD proteins of E. coli. Molec. Microbiol. 4:123-128.Frey, J. and Nicolet, J. (1990) Hemolysin patterns of Actinobacillus pleuropneumoniae. J. Clin. Microbiol. 28:232-236.Strasser, M., Frey, J., Bestetti, G., Kobisch M. and Nicolet J. (1991) Cloning and expression of a species-specificearly immunogenic 36-Kilodalton protein of Mycoplasma hyopneumoniae in Escherichia coli. Infect. Immun.59:1217-1222Frey, J., Deillon, J.-B., Gygi, D. and Nicolet, J. (1991) Identification and partial characterization of the hemolysin(HlyII) of Actinobacillus pleuropneumoniae serotype 2. Vet. Microbiol. 28:303-312Frey, J. and Nicolet J. (1991) Immunological properties of Actinobacillus pleuropneumoniae Hemolysin I. Vet.Microbiol. 28:61-73.Bhatia, B., Mittal, K.R. and Frey, J. (1991) Factors involved in immunity against Actinobacillus pleuropneumoniaein mice. Vet. Microbiol. 28:147-158.Frey, J., Meier, R., Gygi, D. and Nicolet J. (1991) Nucleotide sequence of the Hemolysin I gene fromActinobacillus pleuropneumoniae. Infect. Immun. 59:3026-3032Stipkovits, L., Nicolet, J., Haldimann, A. and Frey, J. (1991) Use of antibodies against the P36 protein ofMycoplasma hyopneumoniae <strong>for</strong> the identification of M. hyopneumoniae strains. Mol. Cell. Probe 5:451-457.Frey, J., Haldimann, A., and Nicolet, J. (1992) Chromosomal Heterogeneity of various Mycoplasmahyopneumoniae field strains. Int. J. Syst. Bacteriol. 42:275-280.Frey, J. (1992) Construction of a broad host range shuttle vector <strong>for</strong> gene cloning and expression inActinobacillus pleuropneumoniae and other Pasteurellaceae. Res. Microbiol. 143:263-269.Frey, J., Bagdasarian, M. M., and Bagdasarian, M. (1992) Replication and copy number control of the broadhost-rangeplasmid RSF1010. Gene 113:101-106.Frey, J., van den Bosch, H., Segers, R., and Nicolet, J. (1992) Identification of a second hemolysin (HlyII) inActinobacillus pleuropneumoniae serotype 1 and expression of the gene in Escherichia coli. Infect. Immun.60:1671-1676.Gygi, D., Nicolet, J. Hughes, C. and Frey, J. (1992) Functional analysis of the Ca 2+ regulated hemolysin I operonof Actinobacillus pleuropneumoniae serotype 1. Infect. Immun. 60:3059-3064.Frey, J., Beck, M., Stucki, U. and Nicolet, J. (1993) Analysis of hemolysin operons in Actinobacilluspleuropneumoniae. Gene 123:51-58.Haldimann, A., Nicolet, J. and Frey, J. (1993) DNA sequence determination and biochemical analysis of theimmunogenic protein P36, the lactate dehydrogenase (LDH) of Mycoplasma hyopneumoniae. J. Gen. Microbiol.139:317-323.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 7/28
Frey, J., Bosse, J.T., Chang, Y.-F., Cullen, J.M., Fenwick B., Gerlach, G.F., Gygi, D., Haesebrouck, F., Inzana,T.J., Jansen, R., Kamp, E.M., Macdonald, J., MacInnes, J.I., Mittal, K.R., Nicolet, J., Rycroft, A.N., Segers,R.P.A.M., Smits, M.A., Stenbaek, E., Struck, D.K., van den Bosch, J.F., Willson, P.J., and Young, R. (1993)Actinobacillus pleuropneumoniae RTX-toxins: uni<strong>for</strong>m designation of haemolysins, cytolysins, pleurotoxin andtheir genes. J. Gen. Microbiol. 139:1723-1728.Frey, J., Haldimann, A., Nicolet, J., Boffini, A. and Prentki, P. (1994) Sequence analysis and transcription of theapxI operon (hemolysin I) from Actinobacillus pleuropneumoniae. Gene 142:97-102.Dolf, G., Hübscher, K.J., Gaillard, C., Stranzinger, G. and Frey, J. (1994) Repetitive plasmid sequences generateDNA fingerprinting patterns in mammals. Animal Genetics 25:273-275.Beck, M., van den Bosch, J.F., Jongenelen, I.M.C.A., Loeffen, P.L.W., Nielsen, R., Nicolet, J., and Frey, J.(1994). RTX toxin genotypes and phenotypes in Actinobacillus pleuropneumoniae field strains. J. Clin. Microbiol.32:2749-2754.Frey, J., Haldimann, A., Kobisch, M., and Nicolet, J. (1994) Immune response against the L-lactatedehydrogenase of Mycoplasma hyopneumoniae in enzootic pneumonia of swine. Microb. Pathog. 17:313-322.Frey, J., Kuhn, R., and Nicolet J. (1994) Association of the CAMP phenomenon in Actinobacilluspleuropneumoniae with the RTX toxins ApxI, ApxII and ApxIII. FEMS Microbiol. Lett. 124:245-252.Huebscher, K.J., Dolf, G., Frey, J., Stranzinger, G., and Gaillard, C. (1995) Features of the DNA fingerprintingprobe pITZ1. Animal Genetics 26:27-30.Stucki, U., Frey, J., Nicolet, J., and Burnens, A.P. (1995) Identification of Campylobacter jejuni based on aspecies specific gene that encodes a membrane protein. J. Clin. Microbiol. 33:855-859.Reimer, D., Frey, J., Jansen, R., Veit, H.P., and Inzana, T. (1995) Molecular investigation of the role of ApxI andApxII in the virulence of Actinobacillus pleuropneumoniae. Microb. Pathog. 18:197-209.Buogo, C., Capaul, S., Häni, H., Frey, J., and Nicolet, J. (1995) Diagnosis of Clostridium perfringens Type Centeritidis in pigs using a DNA amplification technique (PCR). J. Vet. Med. B. 42:51-58.Burnens, A.P., Wagner, J,. Lior, H., Nicolet, J. and Frey, J. (1995) Restriction fragment length polymorphismsamong the flagellar genes of the Lior heat-labile serogroup reference strains and field strains of Campylobacterjejuni and C. coli. Epidemiol. Infect. 114: 423-431.Frey, J., Cheng, X., Kuhnert, P. and Nicolet, J. (1995) Identification and characterization of IS1296 inMycoplasma mycoides subsp. mycoides SC and presence in related mycoplasmas. Gene 150:95-100.Frey, J. (1995) Virulence in Actinobacillus pleuropneumoniae and RTX toxins. Trends in Microbiology 3:257-261.Frey, J., Beck, M., van den Bosch J.F., Segers, R.P.A.M., and Nicolet, J. (1995) Development of an efficientPCR method <strong>for</strong> toxin typing of Actinobacillus pleuropneumoniae strains. Mol. Cell. Probe 9:277-282.Cheng, X., Nicolet, J., Poumarat, F., Regalla, J., Thiaucourt, F., and Frey, J. (1995) Insertion element IS1296 inMycoplasma mycoides subsp. mycoides small colony identifies a European clonal line distinct from African andAustralian strains. Microbiology 141:3221-3228.Burnens, A., Stucki, U., Nicolet, J., and Frey, J. (1995) Identification and characterization of an immunogenicouter membrane protein of Campylobacter jejuni. J. Clin. Microbiol. 33:2826-2832.Kuhnert, P., Nicolet, J. and Frey, J. (1995) Rapid and accurate identification of Escherichia coli K-12 strains.Appl. Environ. Microbiol. 61: 4135-4139.Frey, J., Kuhnert, P., Villiger, L. and Nicolet J. (1996) Cloning and characterization of an Actinobacilluspleuropneumoniae outer membrane protein belonging to the family of PAL lipoproteins. Res. Microbiol. 147:351-361.Frey, J., Beck, M. and Nicolet J. (1996) Typisierung der Apx-Toxin Gene von Actinobacillus pleuropneumoniaemittels PCR. Schweiz. Arch. Tierheilk. 138: 121-124.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 8/28
Miserez, R., Frey, J., Krawinkler, J. and Nicolet J. (1996) Identifikation und Diagnostik von Taylorellaequigenitalis mittels einer DNA-Amplifikationsmethode PCR. Schweiz. Arch. Tierheilk. 138: 115-120.Kuhnert, P., Capaul, S., Nicolet J., and Frey, J. (1996) Phylogenetic positions of Clostridium chauvoei andClostridium septicum based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 46: 1174-1176.Maier, E., Reinhard, N., Benz, R. and Frey, J. (1996) Channel-<strong>for</strong>ming activity and channel size of the RTX toxinsApxI, ApxII and ApxIII of Actinobacillus pleuropneumoniae. Infect. Immun. 64: 4415-4423.Chin, N., Frey, J., Chang, C.-F., and Chang, Y.-F. (1996) Identification of a locus involved in the utilization of ironby Actinobacillus pleuropneumoniae, FEMS Microbiol. Lett. 143: 1-6.Blum, G., Mühldorfer, I., Kuhnert, P., Frey, J. and Hacker J. (1996) Comparative methodology to investigate thepresence of Escherichia coli K-12 strains in environmental and human stool samples. FEMS Microbiol. Lett. 143:77-82.Cheng, X., Nicolet, J., Miserez, R., Kuhnert, P., Krampe, M., Pilloud, Th., Abdo. M., Griot, Ch. and Frey, J. (1996)Characterisation of the gene <strong>for</strong> an immunodominant 72 kDa lipoprotein of Mycoplasma mycoides subsp.mycoides small colony type. Microbiology, 142: 3515-3524.Kuhnert, P., Hacker, J., Mühldorfer, J., Burnens, A.P., Nicolet, J. and Frey, J. (1997) Detection system <strong>for</strong>Escherichia coli-specific virulence genes: Absence of virulence determinants in B and C strains. Appl. Environ.Microbiol. 63:703-709.Subramaniam, S., Chua, K.-L., Tan, H.-M., Loh, H., Kuhnert, P., and Frey, J. (1997) Phylogenetic position ofRiemerella anatipestifer based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 47:562-565.Miserez, R., Pilloud, T., Cheng, X., Nicolet, J., Griot, C. and Frey, J. (1997) Development of a sensitive nestedPCR method <strong>for</strong> the specific detection of Mycoplasma mycoides subsp. mycoides SC. Mol. Cell. Probe 11:103-111.Van Ostaaijen, J., Frey, J., Rosendal S. and MacInnes, J. (1997) Actinobacillus suis strains isolated from healthyand diseased swine are clonal and carry apxICABD var.suis and apxIICA var.suis toxin genes. J. Clin. Microbiol.35:1131-1137.Kuhnert, P., Heyberger-Meyer, B., Burnens, A., Nicolet, J. and Frey, J. (1997) Detection of RTX toxin genes inGram-negative bacteria with a set of specific probes. Appl. and Environ. Microbiol. 63:2258-2265.Frey, J. and Nicolet J. (1997) Molecular identification and epidemiology of animal mycoplasmas. Wien. Klin.Wochenschr. 109:600-603.Kuhnert, P., Krampe, M., Capaul, S.E., Frey, J. and Nicolet, J. (1997) Identification of Clostridium chauvoei incultures and clinical material from blackleg using PCR. Vet. Microbiol. 51:291-298.Abdo. E.-M., Nicolet, J,. Miserez, R., Gonçalves, R., Regalla, J., Griot, C., Bensaide, A., Krampe, M. and Frey, J.(1998) Humoral and bronchial immune responses in cattle experimentally infected with Mycoplasma mycoidessubsp. mycoides small colony type. Vet. Microbiol. 59: 109-122.Frey, J., Cheng, X., Monnerat, M.-P., Abdo, E.-M., Krawinkler, M., Bölske, G. and Nicolet, J. (1998) Genetic andserological analysis of the immunogenic 67-kDa lipoprotein of Mycoplasma sp. bovine group 7. Res. Microbiol.149: 55-64.Stärk, K.D.C., Nicolet, J. and Frey, J. (1998) Detection of Mycoplasma hyopneumoniae by air sampling with anested PCR assay. Appl. and Environ. Microbiol. 64: 543-548.Fellay, R., Hanin, M., Montorzi, G., Frey, J., Freiberg, C., Golinowski, W., Staehelin C., Broughton, W.J. andJabbouri, S. (1998) nodD2 of Rhizobium sp. NGR234 is involved in the repression of the nodABD operon. Molec.Microbiol. 27:1039-1050.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 9/28
Chevallier, B., Dugourd, D., Tarasiuk, K., Harel, J., Gottschalk, M., Kobisch, M. and Frey, J. (1998) Chromosomesizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae. FEMS Microbiol.Lett. 160: 209-216.Giacometti, M., Nicolet, J., Frey, J., Krawinkler, M., Meier, W., Welle, M., Johansson K.-E. and Degiorgis, M.-P.(1998) Susceptibility of alpine ibex to conjunctivitis caused by inoculation of a sheep strain of Mycoplasmaconjunctivae. Vet. Microbiol. 61: 279-288.Subramaniam, S., Bergonier, D., Poumarat, F., Capaul, S., Schlatter, Y., Nicolet, J. and Frey, J. (1998) Speciesidentification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvrC genes by PCR. Mol. Cell.Probe 12:161-169.Miserez, R., Frey, J., Buogo, C., Capaul, S., Tontis, A., Burnens A. and Nicolet, J. (1998) Detection of - and -toxigenic Clostridium perfringens Type D in sheep and goats using a DNA amplification technique (PCR). Lett.Appl. Microbiol. 26:382-386.Mombelli, A., Gmür, R., Frey, J., Meyer, J., Zee, K.Y., Tam, J.O.W., Lo, E.C.M., DiRienzo, J., Lang, N.P. andCorbet, E.F. (1998) Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in young Chineseadults. Oral Microbiol. Immunol. 13: 231-237.Habrun, B., Frey, J., Bilic, V., Nicolet, J. and Humski, A. (1998) Prevalence of serotypes and toxin types ofActinobacillus pleuropneumoniae in pigs in Croatia. Vet. Rec. 143: 255-256.Gonçalves, R., Regalla, J., Nicolet, J., Frey, J., Nicholas, R., Bashiruddin, J., deSantis, P. and Penha-Gonçalves,A. (1998) Antigen heterogeneity among Mycoplasma mycoides subsp. mycoides SC isolates: discrimination ofmajor surface proteins. Vet. Microbiol. 63:13-28.Bagdasarian, M.M., Nagai, M., Frey, J. and Bagdasarian M. (1999) Immunogenicity of Actinobacillus ApxIA toxinepitopes fused to the E. coli heat-labile enterotoxin B subunit. Vaccine 17: 441-447.Herholz, C., Miserez, R., Nicolet, J., Frey, J., Popoff, J., Gibert, M., Gerber, H. and Straub, R. (1999) Prevalenceof b2-toxigenic Clostridium perfringens in horses with intestinal disorders. J. Clin. Microbiol. 37: 358-361.Vilei, E.M., Nicolet, J. and Frey, J. (1999) IS1634, a novel insertion element creating long, variable-length directrepeats which is specific <strong>for</strong> Mycoplasma mycoides subsp. mycoides small colony type. J. Bacteriol. 181: 1319-1323.Monnerat, M.-P., Thiaucourt, F., Poveda, J.B., Nicolet, J. and Frey, J. (1999) Genetic and serological analysis ofLipoprotein LppA in Mycoplasma mycoides subsp. mycoides LC and Mycoplasma mycoides subsp. capri. Clin.Diagn. Lab. Immunol. 6:224-230.Giacometti, M., Nicolet, J., Johansson K.E., Naglic, T., Degiorgis, M.P. and Frey, J. (1999) Detection andidentification of Mycoplasma conjunctivae in infectious keratoconjunctivitis by PCR based on the 16S rRNA gene.J. Vet. Med. B. 46: 173-180.Mombelli, A., Gmür, R., Lang, N.P. Corbet, E., and Frey, J. (1999) Actinobacillus actinomycetemcomitans inChinese adults: Serotype distribution and analysis of the leukotoxin gene promoter locus. J. Clin. Periodontol.26:505-510.Braun, M., Kuhnert, P., Nicolet, J., Burnens, A.P., and Frey, J. (1999) Cloning and characterization of twobistructural S-layer-RTX proteins from Campylobacter rectus. J. Bacteriol. 181: 2501-2506.Klaasen, H.L.B.M., Molkenboer, J.J.C.H., Bakker, J., Miserez, R., Häni, H., Frey, J., Popoff, M.R., Van denBosch, J.F. (1999) Detection of the β2 toxin gene of Clostridium perfringens in diarrhoeic piglets in TheNetherlands and Switzerland. FEMS Immunol. and Med. Microbiol. 24: 325-332.Schaller, A., Kuhn, R., Kuhnert, P., Nicolet, J., Anderson, T. J., MacInnes, J. I., Segers, R. P. A. M. and Frey, J.(1999) Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae. Microbiology145: 2105-2116.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 10/28
Monnerat, M.-P., Thiaucourt, F., Nicolet, J. and Frey, J. (1999) Comparative analysis of the lppA locus inMycoplasma capricolum subsp., capricolum and Mycoplasma capricolum subsp. capripneumoniae. Vet.Microbiol. 69:157-172.Huang, B., Subramaniam, S., Chua, K.-L., Kwang, J., Loh, H., Frey, J. and Tan, H.-M. (1999) Molecularfingerprinting of Riemerella anatipestifer by repetitive sequence PCR. Vet. Microbiol. 67: 213-219.Grattarola, C., Frey, J., Abdo, E.-M., Orusa, R., Nicolet, J. and Giacometti, M. (1999) Mycoplasma conjunctivaeinfections in chamois and ibexes affected with infectious keratoconjunctivitis in the Italian Alps. Vet. Rec. 145:588-589.Rioux, J., Galarneau, C., Harel, J., Frey, J., Nicolet, J., Kobisch, M., Dubreuil, J.D. and Jacques, M. (1999)Isolation and characterization of mini-Tn10 lipopolysaccharide mutants of Actinobacillus pleuropneumoniaeserotype 1. Can. J. Microbiol. 45:1017-1026.Degiorgis, M.-P., Abdo, E.-M., Nicolet, J., Frey, J., Mayer, D., and Giacometti, M. (2000) Immune responses toMycoplasma conjunctivae in alpine ibex, alpine chamois and domestic sheep in Switzerland. J. Wildlife Dis.36:265-271.Kuhnert, P., Heyberger-Meyer, B., Nicolet, J. and Frey, J. (2000) Characterization of PaxA and its operon: acohemolytic RTX toxin determinant from pathogenic Pasteurella aerogenes. Infect. Immun. 68:6-12.Kuhnert, P., Boerlin, P. and Frey, J. (2000) Target genes <strong>for</strong> virulence assessment of Escherichia coli isolatesfrom water, food and the environment. FEMS Microbiol. Rev. 24:107-117.Vilei, E.M., Abdo, E.-M., Nicolet, J., Botelho, A., Gonçalves, R., and Frey, J. (2000) Genomic and antigenicdifferences between the European and African/Australian clusters of Mycoplasma mycoides subsp. mycoidesSC. Microbiology 146:477-486.Braun, M., Herholz, C., Straub, R., Choisat, B., Frey, J., Nicolet, J. and Kuhnert, P. (2000) Detection of the ADPribosyltransferasetoxin gene (cdtA) and its activity in Clostridium difficile isolates from Equidae. FEMS Microbiol.Lett. 184:29-33.Subramaniam, S., Huang, B. Loh, H., Kwang, J., Tan, H.-M., Chua, K-L., and Frey, J. (2000) Characterization ofa predominant immunogenic outer membrane protein of Riemerella anatipestifer. Clin. Diagn. Lab. Immunol.7:168-174.Giacometti, M., Frey, J., Abdo, E.-M., Janovsky, M., Krawinkler, M., Schlatter, Y., Belloy, L., Fatzer, R. andNicolet, J. (2000) Gemsblindheit. Schweiz. Arch. Tierheilk. 142:235-240.Kuhnert, P., Frey, J., Boerlin, P. and Burnens, A.P. (2000) Gensondendiagnostik in der Bakteriologie. Schweiz.Arch. Tierheilk. 142:218-220.Schaller, A., Kuhnert, P., de la Puente-Redondo, V. A., Nicolet, J. and Frey, J. (2000) Apx toxins inPasteurellaceae species from animals. Vet. Microbiol. 74:365-376.Rioux, S., Galarneau, C., Harel, J., Kobisch, J., Frey, J., Gottschalk, M. and Jacques, J. (2000) Isolation andcharacterization of a capsule-deficient mutant of Actinobacillus pleuropneumoniae serotype 1. Microb. Pathog.28: 279-289.Subramaniam, S., Frey, J., Huang, B., Djordjevic, S. and Kwang, J. (2000) Immunoblot assays usingrecombinant antigens <strong>for</strong> the detection of Mycoplasma hyopneumoniae antibodies. Vet. Microbiol. 75: 99-106.Abdo, E.-M., Nicolet, J. and Frey, J. (2000) Antigenic and genetic characterization of Lipoprotein LppQ fromMycoplasma mycoides subsp. mycoides SC. Clin. Diagn. Lab. Immunol. 7: 588-595.Naglic, T., Hajsig, D., Frey, J., Seol, B., Busch, K. and Lojkic, M. (2000) Epidemiological and microbiologicalstudy of an outbreak of infectious keratoconjunctivitis in sheep. Vet. Rec. 147: 72-75.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 11/28
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Jeannnotte, M.-E., Slavic, B., Frey, J., Kuhnert, P. and MacInnes, J.I., (2002) Analysis of non-porcine isolates ofActinobacillus suis. Vet. Microbiol. 85: 83-93.Braun, M., Stuber, K., Schlatter, Y., Wahli, T., Kuhnert, P. and Frey, J. (2002) Characterization of an ADPribosyltransferasetoxin (AexT) from Aeromonas salmonicida subsp. salmonicida. J. Bacteriol. 184:1851-1858.Crasta, K.C., Chua, K.-L., Subramaniam, S., Frey, J., Loh, H. and Tan, H.-M. (2002) Identification andcharacterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer. J. Bacteriol.184: 1932-1939.Giacometti, M., Janovsky, M., Belloy, L. and Frey, J. (2002) Infectious Keratoconjunctivitis of ibex, chamois andother Caprinae. Rev. sci. tech. Off. int. Epiz. 21:335-345.Berthoud, H., Frey, J. and Kuhnert, P. (2002) Characterization of Aqx and its operon: the hemolytic RTXdeterminant of Actinobacillus equuli. Vet. Microbiol. 87:159-174.Garcia Del Blanco, N., Dobson, M.E., Vela, A.I., De La Puente, V.A., Guiterrez, C.B., Hadfield, T.L., Kuhnert P.,Frey, J., Dominguez, L. and Fodriguez Ferri E. F. (2002) Genotyping of Francisella tularensis strains by pulsedfieldgel electrophoresis, amplified fragment length polymorphism fingerprinting and 16S rRNA gene sequencing.J. Clin. Microbiol. 40: 2964-2972.Kuhnert, P., Frey, J., Lang, N.P. and Mayfield, L. (2002) A phylogenetic analysis of Prevotella nigrescens,Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clustering. Int. J. Syst.Evol. Microbiol. 52:1391-1395.Giacometti, M., Janovsky, M., Jenny, H., Nicolet, J., Belloy, L., Goldschmidt-Clermont, E. and Frey, J. (2002)Mycoplasma conjunctivae infection is not maintained in alpine chamois in Eastern Switzerland. J. Wildlife Dis.38:297-304.Frey, J., and Kuhnert, P. (2002) RTX toxins in Pasteurellaceae. Int. J. Med. Microbiol. 292: 149-158.Fleury, B., Bergonier, D., Berthelot, X., Peterhans, E., Frey, J. and Vilei, E.M. (2002) Characterization of P40, acytadhesin of Mycoplasma agalactiae. Infect. Immun. 70: 5612-5621.Huang, B., Kwang, J., Loh, H., Frey, J., Tan, H.-M. and Chua, K.-L. (2002) Development of an ELISA using arecombinant 41 kDa partial protein (P45N') <strong>for</strong> the detection of Riemerella anatipestifer infections in ducks. Vet.Microbiol. 88: 339-349.Burr, S.E., Stuber, K., Wahli, T. and Frey, J. (2002) Evidence <strong>for</strong> a type III secretion system in Aeromonassalmonicida subsp. salmonicida. J. Bacteriol. 184: 5966-5970.van Ijperen, C., Kuhnert, P., Frey, J. and Clewley, J.P. (2002) Virulence typing of Escherichia coli usingmicroarrays. Mol. Cell. Probes 16:371-378.Seah, J.N., Frey, J. and Kwang, J. (2002) The N-terminal Domain of RTX Toxin ApxI of Actinobacilluspleuropneumoniae Elicits Protective Immunity in Mice. Infection & Immunity 70:6464-6467.Pilo, P., Fleury, B., Marenda, M., Frey, J. and Vilei, E.M. (2003) Prevalence and distribution of the insertionelement ISMag1 in Mycoplasma agalactiae. Vet. Microbiol. 92: 37-48.Gottschalk, M., Broes, A., Mittal, K.R., Kobisch, M., Kuhnert, P., Lebrun, A. and Frey, J. (2003) Non-pathogenicActinobacillus isolates antigenically and biochemically similar to Actinobacillus pleuropneumoniae: a novelspecies? Vet. Microbiol. 92:87-101.Kuhnert, P., Berthoud, H., Straub, R. and Frey, J. (2003) Host cell specific activity of RTX toxins from haemolyticActinobacillus equuli and Actinobacillus suis. Vet. Microbiol. 92: 161-167.Belloy, L., Vilei, E. M., Giacometti, M. and Frey, J. (2003) Characterization of LppS, an adhesin of Mycoplasmaconjunctivae. Microbiology 149: 185-193.Djordjevic, S.P., Vilei, E.M. and Frey, J. (2003) Characterization of a chromosomal region of Mycoplasma sp.bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC). Microbiology 149:195-204.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 13/28
Stuber, K., Frey, J., Burnens, A.P. and Kuhnert, P. (2003) Detection of type III secretion genes as a generalindicator of bacterial virulence. Mol. Cell Probes 17: 25- 32.Belloy, L., Janovsky, M., Vilei, E.M., Pilo, P., Giacometti, M. and Frey, J. (2003) Molecular Epidemiology ofMycoplasma conjunctivae in Caprinae: Transmission across Species in Natural Outbreaks. Appl. Environ.Microbiol. 69: 1913-1919.Thoerner, P., Bin Kingombe, C.I., Bögli-Stuber, K., Bissig-Choisat, B., Wassenaar, T.M., Frey, J. and Jemmi, T.(2003) PCR Detection of Virulence Genes in Yersinia enterocolitica and Yersinia pseudotuberculosis andInvestigation of Virulence Gene Distribution. Appl. Environ. Microbiol. 69: 1810-1816.Kuhnert, P., Berthoud, H., Christensen, H., Bisgaard, M. and Frey, J. (2003) Phylogenetic relationship of equineActinobacillus species and distribution of RTX toxin genes among clusters. Vet. Res. 34: 353-359.Motha, M.X.J., Frey, J., Hansen, M.F., Jamaludin, R. and Tham, K.M. (2003) Detection of Mycoplasmaconjunctivae in sheep affected with conjunctivitis and infectious keratoconjunctivitis. New Zealand VeterinaryJournal 51: 186-190.Bacciarini, L.N., Boerlin, P., Straub, R., Frey, J. and Gröne A. (2003) Immunohistochemical localization ofClostridium perfringens ß2-toxin in the gastrointestinal tract of horses. Vet. Pathol. 40: 376-381.Stuber, K., Burr, S.E., Braun, M., Wahli, T. and Frey, J. (2003) Type III secretion genes in Aeromonassalmonicida subsp. salmonicida are located on a large thermolabile virulence plasmid. J. Clin. Microbiol. 41:3854-3856.Frey, J. (2003) Analysis of pathogenic bacteria by gene chip technology. Focal Point: Biotechnology, organizedby F. Hoffmann-La Roche Ltd., Basel, Oct. 16, 2002. Chimia 57: 19.Van den Bosch, H. and Frey, J. (2003) Interference of outer membrane protein PalA with protective immunityagainst Actinobacillus pleuropneumoniae infections in vaccinated pigs. Vaccine 21: 3601-3607.Burr, S.E., Stuber, K. and Frey, J. (2003) The ADP-ribosylating toxin, AexT, from Aeromonas salmonicida subsp.salmonicida is translocated via a Type III secretion pathway. J. Bacteriol. 185: 6583-6591.Pilo, P., Martig, S., Frey, J., and Vilei, E.M. (2003) Antigenic and genetic characterisation of lipoprotein LppCfrom Mycoplasma mycoides subsp. mycoides SC. Vet. Res. 34: 761-775.Burr, S.E., Wahli, T, Segner, H., Pugovkin, D., and Frey, J. (2003) Association of Type III secretion genes withvirulence of Aeromonas salmonicida subsp. salmonicida. Dis. Aquat. Org. 57: 167-171.Mbulu, R.-S., Tjipura-Zaire, G., Lelli, R. Frey, J. Pilo, P., Vilei, E.M., Mettler, F., Nicholas R.A.J., and Huebschle,O.J.B. (2004) Contagious bovine pleuropneumonia (CBPP) caused by vaccine strain T1/44 of Mycoplasmamycoides subsp. mycoides SC. Vet. Microbiol. 98:229-234.Marenda, M.S., Vilei, E.M., Poumarat, F., Frey, J. and Berthelot, X. (2004) Validation of the suppressivesubtractive hybridization method in Mycoplasma agalactiae species by the comparison of a field strain with typestrain PG2. Vet. Res. 35: 199-212.Hüssy, D., Schlatter , Y., Miserez, R., Inzana, T. and Frey, J. (2004) PCR-based identification of serotype 2isolates of Actinobacillus pleuropneumoniae biovars I and II. Vet. Microbiol. 99: 307-310.<strong>Dr</strong>eyfus, A., Schaller, A., Nivollet, S., Segers, R.P.A.M., Kobisch, M., Mieli, L., Soerensen, V., Hüssy, D., Miserez,R., Zimmermann, W., Inderbizin, F. and Frey, J. (2004) Use of recombinant ApxIV in serodiagnosis ofActinobacillus pleuropneumoniae infections, development and prevalidation of the ApxIV ELISA. Vet. Microbiol.99: 227-238.Berthoud, H., Frey, J., Sternberg, S., Straub, R., and Kuhnert, P. (2004) Antibodies against the Aqx toxin ofActinobacillus equuli in horses and foals. Veterinary Record 155: 231-233Korczak, B., Christensen, H., Emler, S., Frey, J. and Kuhnert, P. (2004) Phylogeny of the family Pasteurellaceaebased on rpoB sequences. Int. J. Systematic & Environm. Microbiol. 54: 1393-1399.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 14/28
Dubosson, C.R., Conzelmann, C., Miserez, R., Boerlin, P., Frey, J., Zimmermann, W., Häni, H. and Kuhnert, P.(2004) Development of two real-time PCR assays <strong>for</strong> the detection of Mycoplasma hyopneumoniae in clinicalsamples. Vet. Microbiol. 102:55-65.Wettstein, K. and Frey, J. (2004) Vergleich der antimikrobiellen Resistenz von ausgewählten Infektionserregerndes Respirationstraktes bei verschiedenen Tierarten. Schweiz. Arch. Tierheilk. 146:417-422.Vilei, E.M. and Frey, J. (2004) Differential clustering of Mycoplasma mycoides subsp. mycoides SC strains byPCR-REA of the bgl locus. Vet. Microbiol. 100: 283-288.Thomas, A., Linden, A., Mainil, J., Dizier, I., Baseman, J.B., Kannan, T. R., Fleury, B., Frey, J., Vilei, E. M. (2004)The p40* adhesin pseudogene of Mycoplasma bovis. Vet. Microbiol. 104: 213-217.Thomas, A., Dizier, I., Linden, A., Mainil, J., Frey, J., Vilei, E.M. (2004) Conservation of the uvrC gene sequencein Mycoplasma bovis and its use in routine PCR diagnosis. The Veterinary Journal 168: 100-102.Kuhnert, P., Korczak, B., Falsen, E., Straub, R., Hoops, A., Boerlin, P., Frey, J. and Mutters, R. (2004) Nicoletellasemolina gen. nov., sp. nov., a new member of Pasteurellaceae isolated from horses with airway disease. J. Clin.Microbiol. 42: 5542 - 5548.Korczak, B., Frey, J., Schrenzel, J., Pluschke, G., Pfister. R., Ehricht, R. and Kuhnert P. (2005) Use of diagnosticmicroarrays <strong>for</strong> determination of virulence gene patterns of Escherichia coli K1, a major cause of neonatalmeningitis. J. Clin. Microbiol. 43: 1024-1031.Wahli, T., Burr, S.E., Pugovkin, D., Mueller, O. and Frey, J. (2005) Aeromonas sobria, a causative agent ofdisease in farmed perch, Perca fluviatilis L. J. Fish Dis. 28: 141-150.Bashiruddin, J.B., Frey, J., Heltander Königsson M., Johansson, K.-E., Hotzel, H., Diller, R., de Dantis, P.,Botelho, A., Ayling, R.D., Nicholas, R.A.J. Thiaucourt T. and Sachse, K. (2005) Evaluation of PCR systems <strong>for</strong>the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: A collaborative trial. TheVeterinary Journal 169: 268-275Thomas, A., Linden, A., Mainil, J., Bischof, D.F., Frey, J. and Vilei, E.M. (2005) Mycoplasma bovis sharesinsertion sequences with Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides SC. FEMSMicrobiol. Lett. 245: 249-255.Perreten, V., Vorlet-Fawer, L., Slickers, P., Ehricht, R., Kuhnert, P. and Frey, J. (2005) Microarray-baseddetection of 90 antibiotic resistance genes of Gram-positive bacteria. J. Clin. Microbiol. 43:2291-2302.Kuhnert, P., Schlatter, Y. and Frey, J. (2005) Characterization of the type I secretion system of the RTX toxinApxII in Actinobacillus porcitonsillarum. Vet. Microbiol. 107:225-232.Janis, C., Lartigue, D., Frey, J., Wroblewski, H., Thiaucourt, F., Blanchard, A. and Sirand-Pugnet, P. (2005)Versatile use of oriC plasmids <strong>for</strong> functional genomics of Mycoplasma capricolum subsp. capricolum. Appl.Environ. Microbiol. 71: 2888-2893.Burr, S., Pugovkin, D., Wahli, T., Segner, H. and Frey, J. (2005) Attenuated virulence of an Aeromonassalmonicida subsp. salmonicida type III secretion mutant in a rainbow trout model. Microbiology 151: 2111-2118.Tschopp, R., Frey, J., Zimmermann, L. and Giacometti M. (2005) Outbreaks of infectious keratoconjunctivitis inalpine chamois and ibex in Switzerland between 2001 and 2003. Vet. Rec. 157:13-18.Vilei, E.M., Schlatter, Y., Perreten, V., Straub, R., Popoff, M., Gibert, M., Groene, A., and Frey, J. (2005)Antibiotic-induced expression of a cryptic cpb2 gene in equine β2-toxigenic Clostridium perfringens. Molec.Microbiol. 57: 1570-1581Pilo, P., Vilei, E. M., Peterhans, E., Bonvin-Klotz, L., Stoffel, M. H., Dobbelaere, D. and Frey, J., (2005) Ametabolic enzyme as a primary virulence factor of Mycoplasma mycoides subsp. mycoides Small Colony. J.Bacteriol. 187 (19): 6824-6831.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 15/28
Johansson, A., Engström, B.E., Frey, J., Johansson, K-E. and Båverud, V. (2005) Survival of Clostridiumperfringens during simulated transport and stability of some plasmid-borne toxin genes under aerobic conditions.Acta vet. scand. 46:241-247.Lüdi, S., Frey, J., Favre, D. and Stoffel, M.H. (2006) Assessing the expression of enterotoxigenicEscherichia coli-specific surface antigens in recombinant strains by transmission electron microscopy andimmunolabeling. J. Histochimistry & Cytochemistry 54: 473-477.Korczak, B.M., Stieber, R., Emler, S., Burnens, A.P., Frey, J. and Kuhnert, P. (2006) Genetic Relatedness withinthe genus Campylobacter inferred from rpoB sequences. Int. J. Syst. & Evolut. Microbiol. 56: 937-945.Favre, D., Lüdi, S., Stoffel, S., Frey, J., Horn, M.P., Dietrich, G., Spreng, S. and Viret, J.-F. (2006) Expression ofenterotoxigenic Escherichia coli colonization factors in Vibrio cholerae. Vaccine 24: 4354-4368.Kuehni Boghenbor, K., On S.L.W., Kokotovic, B., Baumgartner, A., Wassenaar, T. M., Wittwer, M., Bissig-Choisat, B. and Frey, J. (2006) Genotyping of Human and Porcine Yersinia enterocolitica, Yersinia intermediaand Yersinia bercovieri Strains from Switzerland by Amplified Fragment Length Polymorphism (AFLP) analysis.Appl. Environ. Microbiol. 72: 4061-4066.Fehr, D., Casanova, C., Liverman, A., Blazkova, H., Orth, K., Dobbelaere, D., Frey, J., and Burr, S.E., (2006)AopP, a Type Three Effector Protein of Aeromonas salmonicida, inhibits the NF-kappaB signalling pathway.Microbiology 152:2809-2828.Tamborrini, M., Werz, D.B., Frey, J., Pluschke, G. and Seeberger, P.H. (2006) Anti-carbohydrate antibodies <strong>for</strong>the detection of anthrax spores. Angew. Chem. Int. Ed. 45:1-3Vilei, E.M., Korczak, B.M. and Frey, J. (2006) Mycoplasma mycoides subsp. capri and Mycoplasma mycoidessubsp. mycoides LC belong to the same subspecies. Vet. Res. 37: 779-790Mayor, D., Korczak, B.M., Christensen, H., Bisgaard, M., Frey, J. and Kuhnert, P. (2006) Distribution of RTXtoxin genes in strains of [Actinobacillus] rossii and [Pasteurella] mairii. Vet. Microbiol. 116: 194-201.Maho, A., Rossano, A., Hächler, H., Holzer, A., Schelling E., Zinsstag, J., Hassane, M.H., Toguebaye, B.S.,Akakpo, A., Van Ert, M., Keim , P., Kenefic, L., Frey, J. and Perreten, V. (2006) Antibiotic susceptibility andmolecular diversity of Bacillus anthracis strains in Chad: Detection of a new phylogenetic subgroup. J. Clin .Microbiol 44: 3422-3425.Bischof, D.F., Vilei, E.M. and Frey, J. (2006) Genomic differences between type strain PG1 and field strains ofMycoplasma mycoides subsp. mycoides small-colony type. Genomics 88: 633-641.Gerber, V., Straub, R., and Frey, J. (2006) Equine Botulism and Acute Pasture Myodystrophy: New soil-borneemerging diseases in Switzerland? Schweiz. Arch. Tierheilk. 148: 553-559.Baenziger, S., Tussiwand, R., Schlaepfer, E., Mazzucchelli, L., Heikenwalder, M., Kurrer, M.E., Behnke, S.,Frey J., Oxenius, A., Joller, H., Aguzzi, A., Manz, M., Speck, R. (2006) Disseminated and sustained HIV-infectionin CD34 + Cord blood cell transplanted Rag2 -/- c -/- mice. PNAS 43: 15951-15956.Vilei, E.M., Correia I., Ferronha M.H., Bischof, D.F., and Frey, J. (2007) β-D-Glucoside utilization by Mycoplasmamycoides subsp. mycoides SC: possible involvement in the control of cytotoxicity towards bovine lung cells BMCMicrobiology 7:31Mayor, D., Zeeh, F., Frey, J. and Kuhnert, P. (2007) Diversity of Mycoplasma hyopneumoniae in pig farmsrevealed by direct molecular typing of clinical material. Vet. Res. 38: 391-398.van den Berg, A., Danuser, J., Frey, J. and Regula G. (2007) Evaluation of the acute phase protein haptoglobinas an indicator of herd health in slaughter pigs. Animal Welfare 16: 157-159.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 16/28
Presi, P., Knopf, L., Regula, G., Pacciarelli, B., Frey, J. and Stärk K.D.C. (2007) Evaluation of the chemicalresidue monitoring in animal-derived products in Switzerland. Food Additives & Contaminants. 24: 590-597.Frey, J. (2007) Biological safety concepts of genetically modified live bacterial vaccines. Vaccine 25: 5598-5605.Vilei, E.M., Bonvin-Klotz L., Zimmermann, L., Ryser-Degiorgis, M.P., Giacomietti, M., F. and Frey, J. (2007)Validation and diagnostic efficacy of a TaqMan real-time PCR <strong>for</strong> the detection of Mycoplasma conjunctivae inthe eyes of infected Caprinae. J. Microbiol. Methods 70: 384-386.Burr, S.E. and Frey, J. (2007) Analysis of type III effector genes in typical and atypical Aeromonas salmonicida.J. Fish. Diseases 30: 711 - 714.Froquet, R., Cherix, N., Burr, S.E., Frey, J., Vilches, S., Tomas, J.M. and Cosson P. (2007) Alternative hostmodel to evaluate Aeromonas virulence. Appl. Environ. Microbiol. 73: 5657 - 5659.Abril, C., Vilei, E.M., Brodard, I., Burnens, A., Frey, J. and Miserez, R. (2007) Discovery of insertion elementISCfe1: a new tool <strong>for</strong> Campylobacter fetus subspecies differentiation. Clin Microbiol Infect. 13: 993-1000.Fehr, D., Burr, S.E., Gibert, M., d’Alayer, J., Frey, J. and Popoff, M.R. (2007) Aeromonas Exoenzyme T ofAeromonas salmonicida is a bifunctional protein that targets the host cytoskeleton. J Biol Chem. 282:28843-28852Frey, J., Eberle, S., Stahl, C., Mazuet, C., Popoff, M., Schatzmann, E., Gerber, V., Dungu, B. and Straub, R.(2007) Alternative vaccination against equine botulism (BoNT/C). Equine Veterinary Journal 39: 516-520Pilo, P., Frey, J and Vilei, E.M. (2007) Molecular mechanisms of pathogenicity of Mycoplasma mycoides subsp.mycoides SC. The Veterinary Journal 174: 513-521.Larsen J., Kuhnert P., Frey J., Christensen, H,. Bisgaard, M. and Olsen, J.E. (2007) Analysis of gene order datasupports vertical inheritance of the leukotoxin operon and genome rearrangements in the 5’ flanking region ingenus Mannheimia. BMC Evolutionary Biol. 7:184.Lüdi S., Frey, J., Favre, D., Viret, J.-F., Ziethlow, V., Kühni-Boghenbor, K. and Stoffel, M.H. (2008) Morphologicaland immunecytochemical analysis of Escherichia coli-specific surface antigens in wildtype strains and inrecombinant Vibrio cholerae. Antonie van Leeuwenhoek;93:185-92.Abril C, Nimmervoll H, Pilo P, Brodard I, Korczak B, Markus S, Miserez R, Frey J. (2008) Rapid diagnosis andquantification of Francisella tularensis in organs of naturally infected common squirrel monkeys (Saimirisciureus).Vet. Microbiol. 127:203-208.Bischof DF, Janis C, Vilei EM, Bertoni G, Frey J.(2008) Cytotoxicity of Mycoplasma mycoides subsp. mycoidesSC toward bovine epithelial cells. Infect Immun. 76: 263-269.Bonvin-Klotz L., Vilei, E.M., Kühni-Boghenbor, K., Kapp, N., Frey, J., and Stoffel, M.H. (2008) Domain analysis oflipoprotein LppQ in Mycoplasma mycoides subsp. mycoides SC. Antonie van Leeuwenhoek 93: 175-183.Albini S., Brodard I., Jaussi A., Wollschlaeger,N., Frey, J. Miserez, R. and Abril, C. (2008) Real-time multiplexPCR assays <strong>for</strong> reliable detection of Clostridium perfringens toxin genes in animal isolates. Vet. Microbiol. 127:179-185.Ziethlow V. Favre D. Viret JF. Frey J. and Stoffel M.H. (2008) Assessment by electron-microscopy ofrecombinant Vibrio cholerae and Salmonella vaccine strains expressing enterotoxigenic Escherichia coli-specificsurface antigens. Clin Microbiol Infect. 14: 276-288.Nussbaumer, I., Miserez, R., Hüssy, D., Doherr, M.G., Frey, J. and Zimmermann W. (2008) Zur Seroprävalenzvon Actinobacillus pleuropneumoniae in schweizerischen Schweinezuchtbetrieben – eine Studie mit dem ApxIVELISA. Schweiz. Arch. Tierheilk. 150: 103-109.Goldschmidt-Clermont E., Wahli T., Frey J. and Burr S.E. (2008) Identification of bacteria from the normal floraof perch, Perca fluviatilis L., and evaluation of their inhibitory potential towards Aeromonas species. J. FishDiseases 30: 353-359.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 17/28
Zimmermann L., Jambresic S. and Frey J. (2008) Specificity of Mycoplasma conjunctivae strains <strong>for</strong> alpinechamois (Rupicapra r. rupicapra). Wildlife Biology 14: 118-124.Pilo P., Perreten V. and Frey J. (2008) Molecular epidemiology of Bacillus anthracis: getting the correct origin.Appl. Environ. Microbiol. 74: 2928-2931.Zhou L., Jones S.C. Angen O., Bossé J.T., Nash J.H., Frey J., Zhou R., Chen HC, Kroll J.S., Rycroft A.N.,Lang<strong>for</strong>d P.R. (2008) A multiplex PCR that can distinguish between immunologically cross-reactive serovar 3, 6and 8 Actinobacillus pleuropneumoniae strains. J Clin Microbiol. 46: 800-803.Zhou L., Jones S.C. Angen O., Bossé J.T., Nash J.H., Frey J., Zhou R., Chen HC, Kroll J.S., Rycroft A.N.,Lang<strong>for</strong>d P.R. (2008) PCR specific <strong>for</strong> Actinobacillus pleuropneumoniae serotype 3 Veterinary Record 162: 648-652.Fournier C., Kuhnert P., Frey J., Miserez R., Kirchhofer M., Kaufmann T., Steiner A., Graber H.U. (2008) BovineStaphylococcus aureus: Association of virulence genes, genotypes and clinical outcome. Res Vet Sci. 85:439-448Presi P., Staerk K., Knopf, L., Breidenbach, E., Sanaa, M., Frey, J. and Regula, G. (2008) Efficiency of riskbasedvs . random sampling <strong>for</strong> the monitoring of tetracycline residues in slaughtered calves in Switzerland. FoodAdditives & Contaminants 25:566-573.Janis C., Bischof D., Gourges G., Frey J., Blanchard, A. and Sirand-Pugnet P. (2008) Unmarked insertionalmutagenesis in the bovine pathogen Mycoplasma mycoides subsp. mycoides SC: characterization of a lppQmutant. Microbiology 154: 2427-2436.Farhat K., Sauter K-S., Brcic M., Frey J., Ulmer, A.J. and Jungi T.W. (2008) The response of HEK293 cellstransfected with bovine TLR2 to established pathogen-associated molecular patterns and to bacteria causingmastitis in cattle. Vet. Immunol. Immunpathol. 125: 326-36.Hinić V., Brodard I., Thomann A., Cvetnić Z., Frey J. and Abril C. (2008) Novel identification and differentiation ofBrucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable <strong>for</strong> both conventional andreal-time PCR systems. Journal of Microbiological Methods 75: 375–378Pósfay-Barbe K.M., Schrenzel J., Frey J., Studer R., Korff C., Belli D.C., Parvex P., Rimensberger P.C., SchäppiM.G. (2008) Food poisoning as a cause of acute liver failure. Pediatr. Infect. Dis. J. 27: 846-847.Dousse F., Thomann A., Brodard I., Korczak B.M., Schlatter Y., Kuhnert P., Miserez, R. and Frey J. (2008)Routine phenotypic identification of bacterial species of the family Pasteurellaceae isolated from animals. J. Vet.Diagn. Invest. 20: 716-724.Filiousis G., Johansson A., Frey J. and Perreten, V. (2009) Prevalence, genetic diversity and antimicrobialsusceptibility of Listeria monocytogenes isolated from open-air food markets in Greece. Food Control 20: 314–317.Arnadottir H., Hvanndal I., Andresdottir V., Burr S.E., Frey, J. and Gudmundsdottir B.K. (2009) The AsaP1peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease(Family M35) and a major virulence factor. J. Bacteriol. 191:403-410.Hames C., Halbedel S., Hoppert M., Frey J. and Stükle J. (2009) Glycerol metabolism is essential <strong>for</strong> cytotoxicityof Mycoplasma pneumoniae. J Bacteriol. 191:747-753.Ryser-Degiorgis M.P., Bischof D.F., Marreros N., Willisch C., Signer C., Filli F., Brosi G., Frey J., Vilei E.M.(2009) Detection of Mycoplasma conjunctivae in the eyes of healthy, free-ranging Alpine ibex: Possibleinvolvement of Alpine ibex as carriers <strong>for</strong> the main causing agent of infectious keratoconjunctivitis in wildCaprinae. Vet Microbiol. 134:368-374.Presi P., Stärk K.D., Stephan R., Breidenbach E., Frey J., Regula G. (2009) Risk scoring <strong>for</strong> setting priorities in amonitoring of antimicrobial resistance in meat and meat products. Int J Food Microbiol. 130:94-100.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 18/28
Tamborrini M., Oberli M.A., Werz D.B., Schürch N., Frey J., Seeberger P.H. and Pluschke G. (2009) Immunodetectionof anthrose containing tetrasaccharide in the exosporium of Bacillus anthracis and Bacillus cereusstrains. J. Appl. Microbiol. Release ahead of publication, PMID: 19226390Bischof, D.F., Vilei E.M. and Frey J. (2009) Functional and antigenic properties of GlpO from Mycoplasmamycoides subsp. mycoides SC: characterization of a FAD-binding site deletion mutant. Vet. Res. 40: 35- .Dedieu, L., Totte P., Rodrigues V., Vilei E.M., Frey J., (2009) Comparative analysis of four lipoproteins fromMycoplasma mycoides subsp. mycoides Small Colony identifies LppA as a major T-cell antigen. Comp. Immunol.Microbiol. Infect. Dis. in press; epub ahead of print PMID: 19187963Manso-Silvan, L., Vilei, E. M., Sachse, K., Djordjevic S. P., Thiaucourt F. and Frey J. (2009) Mycoplasma leachiisp. nov. as a new species designation <strong>for</strong> Mycoplasma sp. bovine group 7 of Leach, and reclassification ofMycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides subsp. capri*. Int J Syst EvolMicrobiol. 59: 1353 – 1358.Gobeli, S., Goldschmidt-Clermont, E., Frey, J. and Burr, S.E. (2009) Pseudomonas chlororaphis strain JF3835reduces mortality of juvenile perch, Perca fluviatilis L., caused by Aeromonas sobria. J. Fish Dis. 32:597-602Calderon-Copete, S., Wigger, G., Wunderlin, C., Schmidheini, T., Frey, J., Quail, M.A. and Falquet, L. (2009)The Mycoplasma conjunctivae genome sequencing, annotation and analysis. BMC Bioin<strong>for</strong>matics 10:doi:10.1186/1471-2105-10-S6-S7Larsen, J., Pedersen, A.G., Davies, R.L., Kuhnert, P., Frey, J., Christensen, H., Bisgaard, M. and Olsen, J.E.(2009) Evolution of the leukotoxin promoter in genus Mannheimia. BMC Evolutionary Biology 9:121doi:10.1186/1471-2148-9-121Burr, S.E. and Frey, J. (2009) Aeromonas salmonicida subsp. salmonicida type strain does not possess a TypeIII secretion system. J. Clin. Microbiol. 47: 3062-3063.Stahl, C., Unger, L., Mazuet, C., Popoff, M., Straub, M. and Frey, J. (2009) Immune response of horses tovaccination with the recombinant HC domain of botulinum neurotoxin types C and D. Vaccine 27:5661-5666.Goldschmidt-Clermont, E., Hochwartner, O., Demarta, A., Caminada, A-P. and Frey, J. (2009) Outbreaks of anUlcerative and Haemorrhagic Disease in Arctic Char (Salvelinus alpinus) caused by Aeromonas salmonicidasubsp. smithia. Diseases of Aquatic Organisms: 86:81-86.Pilo, P., Johansson A. and Frey, J. (2009) Identification of Francisella tularensis cluster in central and westernEurope. Emerging Infectious Diseases 15: 2049-2051.Kuhnert, P., Scholten, E., Haefner, S., Mayor, D. and Frey, J. (2010) Basfia succiniciproducens gen. Nov. sp.nov., a new member of the family Pasteurellaceae isolated from bovine rumen. Int J Syst Evol Microbiol. 60: 44-50.Hinic, V., Brodard, I., Petridou, E., Filioussis, G., Contos, V., Frey, J. and Abril, C. (2010) Brucellosis in a dogcaused by Brucella melitensis Rev1. Vet. Microbiol: 141: 391 – 392.Unger-Torroledo, L., Straub, R., Lehmann, A.D., Graber, F., Stahl, C., Frey, J., Gerber, V., Hoppeler, H., Baum,O. (2010) Lethal toxin of Clostridium sordellii is associated with fatal equine atypical myopathy. Vet. Microbiol.144:487-492.Gurtner, C., Popescu, F., Wyder M., Sutter, E., Zeeh, F., Frey J., von Schubert, C. and Posthaus, H. (2010)Rapid cytopathic Effects of C. perfringens Beta-Toxin on porcine endothelial cells. Infect. Immun 78:2966-2973.Zimmermann, L., Peterhans, E. and Frey, J. (2010).RGD Motif of lipoprotein T, involved in adhesion ofMycoplasma conjunctivae to lamb synovial tissue cells. J. Bacteriol. 192: 3773-3779.Rossetti, B.C., Frey, J. and Pilo, P. (2010) Direct detection of Mycoplasma bovis in milk and tissue samples byreal-time PCR. Molec. and Cell.Probes 24: 321-323CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 19/28
Vilei E. M. and Frey, J. (2010) Detection of Mycoplasma mycoides subsp. mycoides SC in bronchoalveolarlavage fluids of cows based on a TaqMan real-time PCR discriminating wild type strains <strong>for</strong>m an lppQ¯ mutantvaccine strain used <strong>for</strong> DIVA-strategies. J. Microbiological Methods 81:211-218.Smole, I., Thomann, A., Frey J. and Perreten V. (2010) Repression of common bull sperm flora and in vitroimpairment of sperm motility with Pseudomonas aeruginosa introduced by contaminated lubricant. Reprod. DomAnim. 45: 737-742Burr, S.E., Gobeli, S., Kuhnert, P., Goldschmidt-Clermont, E. and Frey, J. (2010) Pseudomonas chlororaphissubsp. piscium subsp. nov., isolated from fresh-water fish. Int. J. Syst. Evol. Microbiol. 60:2753-2757.Holzwarth, N., Pospischil, A., Marreros, N., Ryser-Degiorgis, M-P., Mavrot, F., Frey, J., Thoma, R., Borel, N.(2010) Alpine ibex (Capra i. ibex) is not a reservoir <strong>for</strong> chlamydial infections of domestic ruminants and humans.Eur. J. Wildl. Res. Epub ahead of print doi:10.1007/s10344-010-0416-7.Bürki, S., Vilei, E.M., Wittenbrink, M.M. and Frey, J. (2011) Allelic variations of the nox gene of Brachyspirapilosicoli impair its detection by qPCR. Vet. Microbiol. 149 (2011) 291–292Vilei, E.M., Johansson A., Schlatter, Y., Redhead, K. and Frey, J. (2011) Genetic and functional characterizationof the NanA sialidase from Clostridium chauvoei. Veterinary Research 42:2–9.Abril C., Thomann, A., Brodard, I., Wu N., Ryser-Degiorgis M-P., Frey J., Overesch, G. (2011) A novel isolationmethod of Brucella species and molecular tracking of Brucella suis biovar 2 in domestic and wild animals. Vet.Microbiol. doi:10.1016/j.vetmic.2011.02.056Popescu, F., Wyder, M., Gurnter, C., Frey, J., Cooke, R.S., Greenhill, A.R. and Posthaus H. (2011) Susceptibilityof primary human endothelial cells to C. perfringens beta-toxin suggesting similar pathogenesis in human andporcine necrotizing enteritis. Vet. Microbiol. doi:10.1016/j.vetmic.2011.02.017Frey J. (2011) The role of RTX toxins in host specificity of animal pathogenic Pasteurellaceae. Vet. Microbiol.doi:10.1016/j.vetmic.2011.05.018Tamborrini, M., Bauer, M., Bolz, M., Maho, A., Oberli, M.A., Werz, D.B., Schelling, E., Zinsstag, J., Seeberger,P.H., Frey, J. and Pluschke, G. (2011) Identification of African Bacillus anthracis lineage that lacks expression ofthe spore surface-associated anthrose-containing oligosaccharide. J. Bacteriol. 193:3506-3511.Pilo, P., Frey, J. (2011) Bacillus anthracis: Molecular taxonomy, population genetics, phylogeny and pathoevolution.Infection, Genetics and Evolution, 11:1218-1224.Pilo, P., Rossano, A., Bamanga, H., Abdoulkadiri, S., Perreten V., and Frey, J. (2011) Bovine Bacillus anthracisin Cameroon. Applied and Environmental Microbiology, 77:5818-5821.Blatti, S., Overesch, G., Gerber, V., Frey, J. and Hüssy D. (2011) Seroprevalence of Leptospira spp. in clinicallyhealthy horses in Switzerland. Schweiz. Arch. Tierheilk. 153:449-456Balandyté L., Brodard I., Frey J., Oevermann A and Abril C. (2011) Ruminant rhombencephalitis-associatedListeria monocytogenes alleles linked to a multilocus variable-number tandem repeat analysis complex. ApplEnviron Microbiol. 77:8325-8335.Aebi, M., Bodmer, M., Frey, J. and Pilo, P. (2012) Herd-specific strains of Mycoplasma bovis in outbreaks ofmycoplasmal mastitis and pneumonia. Vet. Microbiol. 157:363-368.Kuhnert, P., Bisgaard, M., Korczak B.M., Schwendener, S., Christensen, H. and Frey, J. (2012) Identification ofanimal Pasteurellaceae by MALDI-TOF mass spectrometry. J. Microbial Methods 89:1–7.Burr, S.E., Goldschmidt-Clermont E., Kuhnert, P. and Frey, J. (2012) Heterogeneity of Aeromonas populations inwild and farmed perch. J. Fish Diseases, in pressFischer, A., Shapiro, B., Muriuki, C., Heller, M., Schnee, C., Bongcam-Rudloff, E., Frey, J. and Jores J. (2012)The origin of the ‘Mycoplasma mycoides cluster’ coincides with domestication of ruminants. PLOS One. Vol. 7,issue 4, e36150CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 20/28
Mavrot, F., Vilei, E.M., Marreros, N., Signer, C., Frey, J. Degiorgis, M.-P. (2012) Occurrence, quantification andgenotyping of Mycoplasma conjunctivae in wild Caprinae with and without infectious keratoconjunctivitis. Journalof Wildlife Diseases. in pressTraoré, S.G., Bonfoh, B., Krabi, R., Odermatt, P., Utzinger, J., Rose, K.-N., Tanner, M., Frey, J., Quilici, M.-L.andKoussémon M. (2012) Risk of Vibrio transmission liked to the consumption of crustacean in coastal towns ofCôte d’Ivoire. J. Food Protection 75:1004-1011Guldimann, C., Lejeune, B., Hofer, S., Leib, S.L. Frey, J., Zurbriggen, A., Seuberlich, T. and Oevermann, A.(2012) Ruminant organotypic brain-slice cultures as a model <strong>for</strong> the investigation of CMS listeriosis. Int. J. Exp.Pathology. in pressKuehni-Boghenbor K., Jordi, H.A., Frey, J., Vilei, E.M, Favre, D. and Stoffel, M. (2012) Expression andmorphology of enterotoxigenic E. coli surface antigen CS31A in E. coli K12 and Vibrio cholerae. Canad. J.Microbiol. 58: 728-73.Frey, J., Johansson, A., Bürki, S., Vilei, E.M., Redhead. K. (2012) Cytotoxin CctA, a major virulence factor ofClostridium chauvoei conferring protective immunity against myonecrosis. Vaccine, 30: 5500-5505.Mavrot, F., Vilei, E.M., Marreros, N., Signer, C., Frey, J. Degiorgis, M.-P. (2012) Occurrence, quantification andgenotyping of Mycoplasma conjunctivae in wild Caprinae with and without infectious keratoconjunctivitis. Journalof Wildlife Diseases. 48: 619–631Scherer, A., Vogt, A-R., Vilei, E.M. Frey, J. and Perreten V. (2013) Enhanced antibiotic multi-resistance in nasaland faecal bacteria after agricultural use of streptomycin. Environmental microbiology, 15: 297-304.Studer, N., Frey, J. and Vanden Bergh P. (2013) Clustering subspecies of Aeromonas salmonicida using IS630typing. BMC Microbiology, 13:36.Küng, E. and Frey, J. (2013) AvxA, a composite serine-protease-RTX toxin of Avibacterium paragallinarum. VetMicrobiol. 163:290-298.Fischer, A., Santana-Cruz, I., Giglio M., Nadendla, S., <strong>Dr</strong>abek, E., Vilei E.M. Frey, J. and Jores, J. (2013)Genome sequence of Mycoplasma feriruminatoris sp. nov., a fast growing Mycoplasma species. GenomeAnnouncements 1: 1 e00216-12.Schmid, S., Seiler, C., Gerecke, A.C., Hächler, H., Hilbi, H., Frey, J., Weidmann, S., Meier, L., Berchtold, C., andZenobi, R. (2013) Studying the fate of non-volatile organic compounds in a commercial plasma air purifier. J.Hazardous Materials 256/257: 76-83.Mair, G., Vilei E.M., Wade A., Frey, J. and Unger H. (2013) Isothermal loop-mediated amplification (LAMP) <strong>for</strong>diagnosis of contagious bovine pleuro-pneumonia. BMC Veterinary Research 9:108Aguilar-Bultet L., Calderon Copete, S.P., Frey, J. and Falquet, L. (2013) <strong>Dr</strong>aft genome sequence of the virulentAvibacterium paragallinarum serotype A strain JF4211 and identification of two toxins. Genome Announcements,1(4): e00592-13.Falquet, L., Calderon-Copete, S.P. and Frey, J. (2013) <strong>Dr</strong>aft genome sequence of the virulent Clostridiumchauvoei reference strain JF4335. Genome Announcements 1(4): e00593-13.Vanden Bergh, P., Burr, S.E., Benedicenti, O., von Siebenthal, B., Frey, J., and Wahli T. (2013) Antigens of thetype-three secretion system of A. salmonicida subsp. salmonicida prevent protective immunity in rainbow trout.Vaccine 32: in pressJores, J., Fischer, A., Sirand-Pugnet, P., Thomann, A., Liebler-Tenorio E.M., Schnee, C., Santana-Cruz, I.,Heller, M. and Frey, J. (2013) Mycoplasma feriruminatoris sp. nov., a fast growing Mycoplasma species isolatedfrom wild Caprinae. Systematic and Applied Microbiology 36: in pressBook Chapters and ReviewsCV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 21/28
Bagdasarian, M., Bagdasarian, M.M., Lurz, R., Nordheim, A., Frey, J. and Timmis, K.N. (1982). Molecular andfunctional analysis of their broad host range plasmid RSF1010 and construction of vectors <strong>for</strong> gene-cloning inGram-negative bacteria. In: Bacterial <strong>Dr</strong>ug Resistance. Mitsuhashi, S. (Ed.). Japan Sci. Soc. Press Tokyo. pp.183-197.Frey, J. and Timmis, K.N. (1984). Use of the BAL 31 exonuclease to map restriction endonuclease cleavagesites in circular genomes. In: Advanced Molecular Genetics. Pühler, A. and Timmis, K.N. (Ed.). Springer Berlin,Heidelberg, New York, Tokyo. pp. 74-79.Frey, J., Bagdasarian, M. and Timmis, K.N. (1984). Generation of deletion mutants in vitro with the BAL 31exonuclease. In: Advanced Molecular Genetics. Pühler, A. and Timmis, K.N. (Ed.). Springer Berlin, Heidelberg,New York, Tokyo. pp. 141-150.Timmis, K.N. and Frey, J. (1984). Cloning with plasmid vectors. In: Advanced Molecular Genetics. Pühler, A. andTimmis, K.N. (Ed.). Springer Berlin, Heidelberg, New York, Tokyo. pp. 160-170.Timmis, K.N. and Frey, J. (1984). Mini-plasmid <strong>for</strong>mation. In: Advanced Molecular Genetics. Pühler, A. andTimmis, K.N. (Ed.). Springer Berlin, Heidelberg, New York, Tokyo. pp. 171-175.Frey, J., Bagdasarian, M. and Timmis, K.N. (1984). Escherichia coli cloning vectors that auto-amplify in thestationary phase of bacterial growth. In: Transferable Antibiotic Resistance: Plasmids and Gene Manipulation.Mitsuhashi, S. and Krcméry, V. (Ed). Avenicum Praha and Springer Heidelberg. pp. 377-383.Bagdasarian, M.M., Scholz, P., Frey, J. and Bagdasarian, M. (1986). Regulation of the rep operon in the broadhost range plasmid RSF1010. In: Antibiotic Resistance Genes: Ecology, Transfer, and Expression. BanburryReport Cold Spring Harbour New York 24: 209-223.Frey, J. and Bagdasarian M. (1989). The molecular biology of IncQ plasmids. In: Promiscuous Plasmids ofGram-negative bacteria. C. Thomas (Ed.) Academic Press Ltd. pp 79-94.Frey, J. (1992) Analyse der Hämolysine von Actinobacillus pleuropneumoniae und ihre Bedeutung in derPathogenese der Schweinepleuropneumonie. Swiss Vet 9 Nr.10:7-16.Huebscher, K.J., Dolf, G. and Frey, J. (1993) Iterons of stringently controlled plasmids and DNA fingerprinting. In:DNA Fingerprinting: State of the Science. S.D.J. Pena, R. Chakraborty, J.T. Epplen and A.J. Jeffreys (Eds.).Birkhäuser Verlag. Basel. pp. 79 - 85.Frey, J. (1994) RTX-toxins in Actinobacillus pleuropneumoniae and their potential role in virulence. In: MolecularMechanisms of Bacterial Virulence. C.I. Kado and J.H. Crosa (Eds.) Kluwer Academic Publishers. pp. 325-340.Frey, J., Beck, M. and Nicolet, J. (1994) RTX-Toxins of Actinobacillus pleuropneumoniae. In: Bacterial ProteinToxins. J. Freer, R. Aitken, J.E. Alouf, G. Boulnois, P. Falmagne, F. Fehrenbach, C. Montecucco, Y. Piemont, R.Rappouli, T. Wadstrom and B. Witholt (Eds). Gustav Fischer, Stuttgart. pp 322-332.Frey, J. (1995) Exotoxins of Actinobacillus pleuropneumoniae. In: Haemophilus, Actinobacillus and Pasteurella.W. Donachie, F.A. Lainson and J.C. Hodgson. (Eds). Plenum, London. pp. 101-113.Frey, J., and MacInnes, J.I. (1995) Genetic manipulations of members of the family Pasteurellaceae. In:Haemophilus, Actinobacillus and Pasteurella. W. Donachie, F.A. Lainson and J.C. Hodgson. (Eds). Plenum,London. pp. 187 - 195.Kuhnert, P. and Frey, J. (1996) Tools <strong>for</strong> Safety Assessment: Identification and Monitoring of Escherichia coli K-12 safety strains. Agency BATS, Basel, ISSSN 1420-228X, pp 1-11.Frey, J., Cheng, X., Poumarat, F., Thiaucourt, F., Regalla, J. and Nicolet J. (1996) Molecular epidemiology ofMycoplasma mycoides subsp. mycoides SC using insertion element IS1296. In: Mycoplasmas of ruminants:pathogenicity, diagnostics, epidemiology and molecular genetics. J. Frey & K. Sarris (Eds.). EuropeanCommission EUR 16934, pp. 21-23.Cheng, X., Nicolet, J., Miserez, R., Kuhnert, P., Krampe, M., Pilloud T., Abdo, M., Griot, C. and Frey, J. (1996)Cloning, expression and analysis of a 72 kDa immunogenic protein from Mycoplasma mycoides subsp. mycoidesSC. In: Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics. J. Frey & K.Sarris (Eds.). European Commission EUR 16934, pp. 24-26.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 22/28
Bergonier, D., Solsana, M., Frey, J., Miserez, R., Nicolet, J., Poumarat, F. (1996) PCR on 16S rRNA gene,restriction endonuclease analysis and SDS page patterns of Mycoplasma agalactiae isolates. In: Mycoplasmas ofruminants: pathogenicity, diagnostics, epidemiology and molecular genetics. J. Frey & K. Sarris (Eds.). EuropeanCommission EUR 16934, pp. 91-93.Miserez, R., Frey, J., Cheng, X., Pilloud, T., Griot, C. and Nicolet, J. (1996) Analysis of clinical samples of cattleexperimentally infected with Mycoplasma mycoides subsp. mycoides SC using a highly sensitive nested PCRmethod. In: Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics. J. Frey& K. Sarris (Eds.). European Commission EUR 16934, pp. 94-96.Miserez, R., Pilloud, T., Krampe, M., Griot, C., Bruckner, L., Blum, J., Tontis, A., Frey, J. and Nicolet, J. (1996)Experimental infections in cattle with a European and an African strain of with Mycoplasma mycoides subsp.mycoides SC. In: Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics. J.Frey & K. Sarris (Eds.). European Commission EUR 16934, pp. 147-149.MacInnes, J.I. and Frey, J. (1997) Actinobacillus suis in healthy and diseased pigs. Proc. Am. Assoc. SwinePractitioners. Québec City, Canada. pp. 471-474.Blum-Oeler, G., Hacker, J., Kuhnert, P. and Frey, J. (1997) Spezifischer Nachweis von Escherichia coli K-12Sicherheitsstämmen mittels PCR. Biospectrum 6:30.Frey, J., Monnerat, M.-P. and Nicolet, J. (1998) Genetic and serological analysis of the major lipoprotein inMycoplasma species of the mycoides cluster. In: Mycoplasmas of ruminants: pathogenicity, diagnostics,epidemiology and molecular genetics, Volume 2. G. Leori, F. Santini, E. Scanziani & J. Frey, (Eds.). EuropeanCommission EUR 18018, pp. 5 - 8.Frey, J., Abdo, E.-M., Miserez, R., Regalla, J., Gonçalves, R., Griot, C., Bensaide, A., Krampe, M. and Nicolet, J.(1998) Humoral and bronchial immune responses in cattle experimentally infected with Mycoplasma mycoidessubsp. mycoides SC. In: Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and moleculargenetics, Volume 2. G. Leori, F. Santini, E. Scanziani & J. Frey, (Eds.). European Commission EUR 18018, pp.40 - 43.Botelho, A., Frey, J., Regalla, J. and Nicolet, J. (1998) DNA fingerprinting of Mycoplasma mycoides subsp.mycoides SC isolates with the insertion element IS1296. In: Mycoplasmas of ruminants: pathogenicity,diagnostics, epidemiology and molecular genetics, Volume 2. G. Leori, F. Santini, E. Scanziani & J. Frey, (Eds.).European Commission EUR 18018, pp. 148 - 151.Naglic, T., Frey, J., Hajsig, D,. Busch, K. and Seol, B. (1998) Epizootiological and microbiological study ofinfectious keratoconjunctivitis in sheep. In: Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiologyand molecular genetics, Volume 2. G. Leori, F. Santini, E. Scanziani & J. Frey, (Eds.). European CommissionEUR 18018, pp.168 - 171.Frey, J. (1998) Insertion Sequence Analysis. In: Methods in Molecular Biology, Mycoplasma Protocols. R.J. Milesand R.A.J. Nicholas Eds. Vol. 104:197-205.Frey, J., Burnens, A., Stuber, K., Nicolet, J. and Kuhnert, P. (1998) Target genes <strong>for</strong> the identification anddetection of potentially hazardous bacteria. In. Molecular Methods <strong>for</strong> Safe <strong>Dr</strong>inking Water. OECD, Paris. Pp 1 –14.Benz, R. and Frey, J. (1998) Channel Size of the RTX-toxins ApxI, ApxII, ApxIII of Actinobacilluspleuropneumoniae as Derived From Lipid Bilayer Experiments. In: Bacterial Protein Toxins. J. Hacker, J.E. Alouf,B.C. Brand, P. Falmagne, F.J. Fehrenbach, J.H. Freer, W. Goebel, R. Gross, J. Heesemann, C. Locht, C.Montecucco, S. Olsnes, R. Rapouli, J. Reidl, T. Wadström (Eds.) Zent. B., Bakteriol. Suppl. 29. Gustav Fischer.Stuttgart, pp 24 - 31.Kuhnert, P., Heyberger-Meyer, B. and Frey, J. (1998) Detection of RTX toxin genes in Gram-negative bacteriaand characterization of a new RTX operon in Pasteurella aerogenes using a specific set of gene probes. In:Bacterial Protein Toxins. J. Hacker, J.E. Alouf, B.C. Brand, P. Falmagne, F.J. Fehrenbach, J.H. Freer, W.Goebel, R. Gross, J. Heesemann, C. Locht, C. Montecucco, S. Olsnes, R. Rapouli, J. Reidl, T. Wadström (Eds.)Zent. B., Bakteriol. Suppl. 29. Gustav Fischer. Stuttgart, pp 403 - 404.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 23/28
Kuhn, R., Anderson, T.J., MacInnes, J., Nicolet, J. and Frey, J. (1998) Characterization of a novel RTXdeterminant, apxIV, in Actinobacillus pleuropneumoniae. In: Bacterial Protein Toxins. J. Hacker, J.E. Alouf, B.C.Brand, P. Falmagne, F.J. Fehrenbach, J.H. Freer, W. Goebel, R. Gross, J. Heesemann, C. Locht, C.Montecucco, S. Olsnes, R. Rapouli, J. Reidl, T. Wadström (Eds.) Zent. B., Bakteriol. Suppl. 29. Gustav Fischer.Stuttgart, pp 51 - 52.Frey, J., Subramaniam, S., Bergonier, D. and Nicolet, J. (1999) DNA repair genes uvrC as genetic targets <strong>for</strong>discrimination of closely related mycoplasmas. In: Mycoplasmas of ruminants: pathogenicity, diagnostics,epidemiology and molecular genetics, Volume 3. L. Stipkovits, R. Rosengarten & J. Frey, (Eds.). EuropeanCommission EUR 18756, pp. 40-43.Monnerat, M.-P., Frey, J., Thiaucourt, F., Poveda, J.B. and Nicolet, J. (1999) Genetic and immunogeniccharacterisation of a 62 kDa lipoprotein (P62) in Mycoplasma mycoides subsp. mycoides LC and Mycoplasmamycoides subsp. capri. In: Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and moleculargenetics, Volume 3. L. Stipkovits, R. Rosengarten & J. Frey, (Eds.). European Commission EUR 18756, pp. 73-76.Fleury, B., Bergonier, D., Sagne, E., Manesse, M., Berthelot, X., Nicolet, J. and Frey, J. (1999) Specific detectionof Mycoplasma agalactiae by PCR and nested-PCR based on the amplification of the uvrC gene. In:Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics, Volume 3. L.Stipkovits, R. Rosengarten & J. Frey, (Eds.). European Commission EUR 18756, pp. 97-100.Frey, J., Kuhnert, P., Stettler, P., and Viret, J.-F. (2000) Development, application and problematics of a generalPCR method <strong>for</strong> Detection of Mycoplasmas in cell cultures. Pharmeuropa, Aug. 2000, pp 33-43.Frey, J., Monnerat, M.-P., Thiaucourt, F. and Nicolet, J. (2000) Analysis of the lipoprotein A gene locus lppA inMycoplasmas of the M. mycoides cluster. In: Mycoplasmas of ruminants: pathogenicity, diagnostics,epidemiology and molecular genetics. Volume 4. D. Bergonier, X. Berthelot & J. Frey (Eds.) EuropeanCommission EUR 19245EN, ISBN 92-828-9830-X, pp 189-192.Clark, J., Frey, J. and March, J.B., (2000) Strain typing and molecular epidemiology of Mycoplasma mycoidessubsp. mycoides small colony by insertion sequence analysis. In: Mycoplasmas of ruminants: pathogenicity,diagnostics, epidemiology and molecular genetics. Volume 4. D. Bergonier, X. Berthelot & J. Frey (Eds.)European Commission EUR 19245EN, ISBN 92-828-9830-X, pp 163-166.Orusa, R., Giacometti, M., Banchi, C., Belloy, L. and Frey, J. (2000) Resultati preliminary sulla pressenza diinfezioni da Mycoplasma conjunctivae negli ovine in valle d’Aosta. Occurrence of Mycoplasma conjunctivae –infections in domestic sheep in the Aosta valley (Italy): Preliminary results. In: Comunicazioni ScientificheRelazioni Simposio: Ruolo dell’allimentazione nel miglioramento delle prestazioni produttive degli ovicaprini.Società Italiana di Patologia e Allevamento degli Ovini e dei Caprini. Vietri sul Mare (SA). pp 119-122.Frey, J., Abdo, E.-M. and Nicolet, J. (2001) Genetic and antigenic characterization of LppQ, a specific lipoproteinof M. mycoides subsp. mycoides SC. In: Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiologyand molecular genetics. Volume 5. J.B. Poveda, A. Fernández, J. Frey & K.E. Johansson (Eds.) EuropeanCommission EUR 19693EN, ISBN 92-829-0612-4, pp 10 - 13.Frey, J. (2002) Mycoplasmas of Animals. In: Molecular Biology and Pathogenicity of Mycoplasmas. S. Razin andR. Herrmann (Eds). Chapter Kluwer Academic/Plenum Publishers, New York, Boston, Dordrecht, London,Moscow pp 73 - 90.Hübschle, O., Lelli, R., Frey, J. and Nicholas, R. (2002) Contagious bovine pleuropneumonia and vaccine strainT 1 /44. Letters, Vet. Rec. 150 (19): 615.Frey, J. (2002) Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae. In: Methods inMolecular Biology, Volume 216: PCR Detection of Microbial Pathogens. K. Sachse & J. Frey (Eds). HumanaPress Totowa, New Jersey. pp 87 – 95.Hotzel, H., Frey, J., Bashiruddin, J. and Sachse, K. (2002) Detection and Differentiation of RuminantMycoplasmas. In: Methods in Molecular Biology Volume 216: PCR Detection of Microbial Pathogens. K. Sachse& J. Frey (Eds). Humana Press Totowa, New Jersey. pp 231 - 245.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 24/28
Kobisch, M. and Frey, J. (2002) Detection of Mycoplasma hyopneumoniae from Clinical Samples and Air. In:Methods in Molecular Biology Volume 216: PCR Detection of Microbial Pathogens. K. Sachse & J. Frey (Eds).Humana Press Totowa, New Jersey. pp. 247 – 256.Frey, J. (2002) Molecular genetics of Clostridia in vitro and in vivo. In: Molecular genetics, classification,pathology and ecology of the genus Clostridium. C. Duchesnes & J. Mainil (Eds). European Concerted ActionQLK 2 -CT2001-01267. pp. 31 – 35.Frey, J., Pilo Paola and Vilei, E.M. (2004) Molecular mechanisms of virulence and antigenicity of Mycoplasmamycoides subsp. mycoides SC: Conclusions <strong>for</strong> prevention and control of CBPP. In: Towards Sustainable CBPPControl Programmes <strong>for</strong> Africa. FAO, Rome ISBN 92-5-105166-6.Nicholas, R.A.J., Tjipura-Zaire, G., Mbulu, R.S., Scacchia, M., Mettler, F,. Frey, J., Abusugra, I., and Huebschle,O.J.B. (2004) An inactivated whole cell vaccine and LppQ subunit vaccine appear to exacerbate the effects ofCBPP in adult cattle. In: Towards Sustainable CBPP Control Programmes <strong>for</strong> Africa. FAO, Rome ISBN 92-5-105166-6.Frey, J. (2006) Genetics and phylogeny of RTX cytolisins. In: The Comprehensive Sourcebook of BacterialProtein toxins J. Alouf and M.R. Popoff (Eds). Elsevier Amsterdam pp. 570 - 577.Frey J. (2007) Mécanisme de l’induction par antibiotique de l’expression du gène cryptique cpb2 de Clostridiumperfringens beta-2 toxigène impliqué dans la typhlocolite du cheval. In: Toxines émergentes: nouveaux risques(F. Goudet-Perrière, E. Beniot, P. Marchot and M. Popoff eds). Lavoisier, Paris, France, pp 83 – 87.Frey J. (2008) RTX toxin-determined virulence of Pasteurellaceae. In: Pasteurellaceae Biology, Genomics andMolecular Aspects (P. Kuhnert and H. Christensen eds). Caister Academic Press, Norfolk, UK. pp133 – 144.Pilo P. and Frey J. (2009) Tularémie: une zoonose rare, sous haute surveillance. In: Rapport suisse sur leszoonoses 2008. Office vétérinaire féderal OVF, Bern, Switzerland.Perreten V. and Frey J. (2009) Use of a microchip to detect antibiotic resistance genes in Bacillus anthracis. In:Detection of Highly Dangerous Pathogens – Microarray Methods <strong>for</strong> BSL3 and BSL4 Agents. T. Kostic, P. Butayeand J. Schrenzel (Eds). Wiley Blackwell, Weinheim Germany, ISBN: 978-3-527-32275-6, pp 147 – 152.Frey J. (2011) Toxina RTX: Fator de virulência do Actinobacillus pleuropneumoniae. Revista Técnica daSuinocultura. Vol 38: 10-17.Antwerpen, M., Pilo, P., Wattiau, P., Butaye, P., Frey, J and Frangoulidis, D. (2012) Bacillus anthracis: Anthrax.In: BSL3 and BSL4 Agents, Epidemiology, Microbiology and Practical Guidelines. M.C. Elschner, S.J. Cutler, M.Weidmann and P. Butaye (Eds). Wiley Blackwell, Weinheim Germany, ISBN 978-3-527-31715-8; pp 5 – 18.Frey J. (2012) Gentechnisch veränderte Pflanzen: Schutzziele und Risikobewertung. In: Nutzen und Risiken derFreisetzung gentechnisch veränderter Pflanzen. Programmsynthesis NFP59. Schweizerischer Nationalfonds zurFörderung der Wissenschaftlichen Forschung. Verlag VDF_ETHZ. ISBN 978-3-7281-3483-7; pp 62 – 73.Frey, J. (2012) Wildbahnassoziierte Zoonosen: Bakterielle Erreger. Riport 70:8 – 9.Books EditedMycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics. J. Frey & K. Sarris(Eds.) (1996) European Commission, EUR 16934, ISBN 92-827-7763-4.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 25/28
Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics. Volume 2. G. Leori,F. Santini, E. Scanziani and J. Frey (Eds.) (1998) European Commission, EUR 18018, ISBN 92-828-1873-X.Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics, Volume 3. L.Stipkovits, R. Rosengarten & J. Frey (Eds.) (1999) European Commission EUR 18756, ISBN 92-828-5222-9.Evolution of bacterial virulence and antibiotic resistance. J.-C. Piffaretti and J. Frey. (Eds) (1999) Cellular andMolecular Life Sciences CMLS Vol. 56 No. 9/10 (Multi-author Review).Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics. Volume 4. D.Bergonier, X. Berthelot & J. Frey (Eds.) (2000) European Commission EUR 19245EN, ISBN 92-828-9830-X.Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics. Volume 5. J.B.Poveda, A. Fernández, J. Frey & K.E. Johansson (Eds.) (2001) European Commission EUR 19693EN, ISBN 92-829-0612-4.Methods in Molecular Biology Volume 216: PCR Detection of Microbial Pathogens. (2002) K. Sachse & J. Frey(Eds). Humana Press Totowa, New Jersey. ISBN 1-58829-049-2.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 26/28
MonographsFrey, J. (1980). The role of the dnaA function in the regulation of DNA replication in Escherichia coli. Thèse No1974, Université de Genève.Frey, J. (1991) Biochemische, genetische und immunologische Analyse des Haemolysins/Zytolysins vonActinobacillus pleuropneumoniae. Habilitationsschrift, Universität Bern, Veterinärmedizinische Fakultät.Other PublicationsFrey, J. (1980). The role of the dnaA function in the regulation of DNA replication in Escherichia coli. Thèse No1974, Université de Genève.Frey, J. (1985) Le génie génétique: une nouvelle discipline de base en biotechnologie. Cahiers de la faculté;Université de Genève 12: 15-21.Frey, J., (1992) Le génie génétique, discipline de base de la biotechnologie. La Revue Polytechnique 1545:151-156.Nicolet, J., Frey, J. and Burnens, A. (1993) Das Institut für Veterinärbakteriologie. LABOLIFE No 3/93 pp. 25 -27.Frey, J. (1996) Mykoplasmosen der Wiederkäuer. In: Europaaktivitäten Berner Hochschulen, A. Bürgi-Schmelzed. Eurocor, Berne, pp 31-32.Frey, J. (1998) Génie Génétique - La médecine vétérinaire à la recherche d’outils plus efficaces. Ingénieurs etArchitectes Suisses (IAS) 12: 194-198.Frey, J. (1998) Biotechverfahren werden sicherer. Focus Biosicherheit No 7: 2-4.Frey, J. (2002) Mikrobiologische und molekulargenetische Analysen von Proben mit Verdacht aufBioterrorismus. Schweizerische Zeitschrift für Militär- und Katastrophenmedizin. 79: 48-51.Frey J. (2003) Analysis of Pathogenic Bacteria by Gene Chip Technology. Focal Point: Biotechnology, organizedby F. Hoffmann-La Roche Ltd., Basel, Oct. 16, 2002. Chimia 57: 19.Juilliand, V., Philippeau, C., Schelcher, F., Popoff, M., and Frey, J. (2003) Les entérotoxémies et les mortssubites des ruminants. Conférence, Lyon, 11 mars 2003.La Semaine Vétérinaire no. 1093, p. 33.CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 27/28
PatentsSegers, R.P.A.M., van den Bosch, J.F. and Frey, J. (1996)Live attenuated RTX-producing bacteria of the family PasteurellaceaeNo. 97201613.3-2105 Priority EP /31.05.96/ EP 96201557 European Patent Office München.Bagdasarian, M. Frey, J. (1997)B Subunit of the Heat Labile Enterotoxin as Epitope-Display Vector and the Basis <strong>for</strong> Recombiant Vaccines.Patent pending, 1997, Michigan State University, East Lansing, Michigan, USA, 48824-1046.Segers, R.P.A.M., Frey, J. (1997)Live attenuated bacteria of the species Actinobacillus pleuropneumoniaeNo. 98201115.7-1270 Priority EP/10.04.97/ EP 97201032 European Patent Office München.Subramaniam, S., Frey, J. (1999)OmpA gene <strong>for</strong> an outer membrane protein of Riemerella anatipestifer and methods of use.Ref. GM/MC/R33-63. Singapore Patents Registry 1999.Frey, J., Nicolet, J., and Abdo, E.M. (2000)Antigenic protein LppQ of Mycoplasma mycoides subsp. mycoides SC: Its preparation and use.No. 99123676.1-2105 European Patent Office, München Publication no. WO 0140471.Frey, J., Kuhnert, P., Braun, M. (2000)Novel exoenzyme toxin of Aeromonas salmonicida.US patent application. Nov. 15, 2000.Frey, J., Kuhnert, P., Braun, M., Thornton, J., Kuzik, M.A., and Burian, J. (2002)Novel exoenzyme toxin of Aeromonas salmonicida and uses there<strong>for</strong>. World Intellectual Property Organization[WIPO/OMPI] International Patent, International Publication number WO 02/40515 A2 (23.05.2002).Frey, J., Stuber, K., M., Thornton, J., Kuzik, M.A., and Burian, J. (2002)Novel Type III secretion pathway in Aeromonas salmonicida and uses there<strong>for</strong>. World Intellectual PropertyOrganization [WIPO/OMPI] International Patent, International Publication number WO 02/40514 A2 (23.05.2002).Frey, J., Pilo, P. and Vilei, E. (2005)Mycoplasma subunit vaccine; Patent Application nr. USSN 11/176700) United States Patent and TrademarkOffice (USPTO) filed on the 7 th of July 2005Frey, J., Kuhnert, P., Thornton, J.C., Kuzik, M.A., Burian, J and Braun, M.,. (2008)Exoenzyme toxin of Aeromonas salmonicida and uses thereof. United States Patent. Patent No.: US 7,351,550B2; April 1 st 2008.Frey, J., Vilei, E.M. and Pilo, P. (2010)Mycoplasma subunit vaccine, United States Patent No US7,833,536 B2, (16 th Nov. 2010).Frey, J., Vilei E.M., Johansson A. (2010)A Clostridium chauvoei polypeptide, DNA encoding the polypeptide and a vaccine comprising the polypeptide.World Intellectual Property Organization [WIPO/OMPI] International Patent, International Publication number WO2010/086353 A1 (05.08.2010).Frey, J., Küng, E. (2013)Avibacterium paragallinarum RTX toxin. World Intellectual Property Organization [WIPO/OMPI]PCT/EP2012/067506 International Patent, International Publication number WO 2013/034687 A1CV <strong>Joachim</strong> <strong>FREY</strong>, Inst. <strong>for</strong> Veterinary Bacteriology, Laenggasstr. 122, CH-3012 Berne; 12.09.13; page 28/28
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