Transformation of E. coli with Green and Blue ... - Frederiksen
Transformation of E. coli with Green and Blue ... - Frederiksen
Transformation of E. coli with Green and Blue ... - Frederiksen
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
<strong>Transformation</strong> <strong>of</strong> E. <strong>coli</strong> <strong>with</strong> <strong>Green</strong> <strong>and</strong><strong>Blue</strong> Fluorescent Protein PlasmidsEDVO-Kit # 222 11Experiment OverviewStreak E. <strong>coli</strong> hostcells for isolationDAY BEFORE LABPrepare 5Source PlatesE. <strong>coli</strong> Cells- DNAPlasmid DNATransfer 2-4 large colonies to eachtube <strong>and</strong> completely resuspend+ DNA+pFluoro<strong>Green</strong> <strong>and</strong>/orpFluoro<strong>Blue</strong>10 µlThe Experiment250 µlCaCl 2Incubate on ice for15 minutesIncubate at 42°Cfor 90 secondsIncubate on icefor 2 minutes- DNAAdd 250 µlLuria Recovery Broth37°C for30 minutes+ DNAControlExperimentLB-LB/Amp - LB+IncubateIncubateLB/Amp +overnight in 37°Cincubation ovenovernight in 37°Cincubation ovenLB- LB/Amp - LB+ LB/Amp +LONG WAVE U.V. LIGHTIS REQUIREDTO OBSERVEFLUORESCENT COLONIES.Duplication <strong>of</strong> this document, in conjunction <strong>with</strong> use <strong>of</strong> accompanying reagents, is permitted for classroom/laboratoryuse only. This document, or any part, may not be reproduced or distributed for any other purpose <strong>with</strong>out thewritten consent <strong>of</strong> EDVOTEK, Inc. Copyright © 1999, 2003, EDVOTEK, Inc., all rights reservedEVT 005077KThe Biotechnology Education Company ® • 1-800-EDVOTEK • www.edvotek.com
Change language