Transformation of E. coli with Green and Blue ... - Frederiksen
Transformation of E. coli with Green and Blue ... - Frederiksen
Transformation of E. coli with Green and Blue ... - Frederiksen
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<strong>Transformation</strong> <strong>of</strong> E. <strong>coli</strong> <strong>with</strong> <strong>Green</strong> <strong>and</strong><strong>Blue</strong> Fluorescent Protein PlasmidsEDVO-Kit # 222 21Instructor’s GuideNotes to the Instructor:Online Orderingnow availableVisit our web site forinformation aboutEDVOTEK's completeline <strong>of</strong> experiments forbiotechnology <strong>and</strong>biology education.EDVO-TECH SERVICEMon - Fri1-800-EDVOTEK(1-800-338-6835)9 am - 6 pm ETImportant READ ME!<strong>Transformation</strong> experiments contain antibiotics which are used for theselection <strong>of</strong> transformed bacteria. Students who have allergies to antibioticssuch as penicilllin, ampicillin, kanamycin or tetracycine shouldnot participate in this experiment.Organizing <strong>and</strong> Implementing the ExperimentClass size, length <strong>of</strong> laboratory sessions, <strong>and</strong> availability <strong>of</strong> equipmentare factors which must be considered in the planning <strong>and</strong> the implementation<strong>of</strong> this experiment <strong>with</strong> your students.The guidelines that are presented in this manual are based on ten laboratorygroups consisting <strong>of</strong> two, or up to four students. The followingare implementation guidelines, which can be adapted to fit your specificset <strong>of</strong> circumstances. If you do not find the answers to your questionsin this section, a variety <strong>of</strong> resources are available at the EDVOTEK website. In addition, Technical Service is available from 9:00 am to 6:00 pm,Eastern time zone. Call 1-800-EDVOTEK for help from our knowledgeabletechnical staff.Technical ServiceDepartmentMon - Fri9:00 am to 6:00 pm ETFAX: (301) 340-0582web: www.edvotek.comemail: edvotek@aol.comPlease have the following information:• The experiment number <strong>and</strong> title• Kit Lot number on box or tube• The literature version number(in lower right corner)• Approximate purchase dateDay 1: (Prior to the Lab)• Prepare agar plates• Prepare E. <strong>coli</strong> Cells (overnight incubation).• Dispense the DNA <strong>and</strong> control bufferDay 2: (Day <strong>of</strong> Lab Experiment)• Equilibrate water baths at 37°C <strong>and</strong>42°C; incubation oven at 37°C• Students transform cells <strong>and</strong> plate forovernight incubation.Day 3: (Day after Lab Experiment)• Students observe transformants <strong>and</strong>controls• Students calculate transformation efficiency• Follow clean up <strong>and</strong> disposal proceduresas outlined in the Laboratory Safety section.EDVOTEK - The Biotechnology Education Company ®1-800-EDVOTEK • www.edvotek.com24-hour FAX: (301) 340-0582 • email: edvotek@aol.comEVT 005077K
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