Thibault Senterre, MD, PhD

Liège protocol with NUMETA300mL Bags: 3-Chamber ActivationTriple Chamber 300 mL D1 D2 D3 D4 D5 ≥ D6Volume 100 mL/kg/d 30 40 50 60 70 80 90 100 110 120 130AA 3.1 g/kg/d 0.9 1.2 1.6 1.9 2.2 2.5 2.8 3.1 3.4 3.7 4Glucose 13.3 g/kg/d 4 5.3 6.7 8 9.3 10.6 12 13.3 14.6 16 17.3Lipids 2.5 g/kg/d 0.8 1 1.3 1.5 1.8 2 2.3 2.5 2.8 3 3.3Energy 85 kCal/kg/d 25.5 34 42.5 51 59.5 68 76.5 85 93.5 102 110.5Na+ 2.1 mmol/kg/d 0.6 0.9 1.1 1.3 1.5 1.7 1.9 2.1 2.3 2.6 2.8K+ 2.1 mmol/kg/d 0.6 0.8 1.1 1.3 1.5 1.7 1.9 2.1 2.3 2.5 2.7Cl- 3.1 mmol/kg/d 0.9 1.2 1.6 1.9 2.2 2.5 2.8 3.1 3.4 3.7 4Ca++ 52 mg/kg/d 15.6 20.8 26 31.2 36.4 41.6 46.8 52 57.2 62.4 67.6P 40 mg/kg/d 12 16 20 24 28 32 36 40 44 48 52Primene 10%AA 1 g/kg/d 1 1 1 0.5 0.5 0.5 0 0 0 0 0Volume 10 mL/kg/d 10 10 10 5 5 5 0 0 0 0 0Energy 3.75 kCal/kg/d 3.8 3.8 3.8 1.9 1.9 1.9 0 0 0 0 0VitalipidVolume max 4 mL/kg/d 1 1 1 1 2 2 2 3 4 4 4Vol. TOTAL mL/kg/d 41 51 61 66 77 87 92 103 114 124 134

Liège protocol with NUMETA300mL Bags: 2-Chamber ActivationTriple Chamber 300 mL D1 D2 D3 D4 D5 ≥ D6Volume 100 mL/kg/d 30 40 50 60 70 80 90 100 110 120 130AA 3.1 g/kg/d 0.9 1.2 1.6 1.9 2.2 2.5 2.8 3.1 3.4 3.7 4Glucose 13.3 g/kg/d 4 5.3 6.7 8 9.3 10.6 12 13.3 14.6 16 17.3Lipids 2.5 g/kg/d 0.8 1 1.3 1.5 1.8 2 2.3 2.5 2.8 3 3.3Energy 85 kCal/kg/d 25.5 34 42.5 51 59.5 68 76.5 85 93.5 102 110.5Na+ 2.1 mmol/kg/d 0.6 0.9 1.1 1.3 1.5 1.7 1.9 2.1 2.3 2.6 2.8K+ 2.1 mmol/kg/d 0.6 0.8 1.1 1.3 1.5 1.7 1.9 2.1 2.3 2.5 2.7Cl- 3.1 mmol/kg/d 0.9 1.2 1.6 1.9 2.2 2.5 2.8 3.1 3.4 3.7 4Ca++ 52 mg/kg/d 15.6 20.8 26 31.2 36.4 41.6 46.8 52 57.2 62.4 67.6P 40 mg/kg/d 12 16 20 24 28 32 36 40 44 48 52Primene 10%AA 1 g/kg/d 1 1 1 0.5 0.5 0.5 0 0 0 0 0Volume 10 mL/kg/d 10 10 10 5 5 5 0 0 0 0 0Energy 3.75 kCal/kg/d 3.8 3.8 3.8 1.9 1.9 1.9 0 0 0 0 0VitalipidVolume max 4 mL/kg/d 1 1 1 1 2 2 2 3 4 4 4Vol. TOTAL mL/kg/d 41 51 61 66 77 87 92 103 114 124 134

Early nutritionD1 D2 D3 D4 D5 D6 D7 D8 D9NUMETA with lipids(mL/kg*d)60 70 100 110 105 80NUMETA withoutlipids (mL/kg*d)40 35 15Vitamins + + + + + + + + +Trace elements + + + + + + + + +Primene (mL/kg*d) 10 10Parenteral (mL/kg*d) 70 80 100 110 105 80 40 35 15Enteral (mL/kg*d) 0 15 26 34 54 72 97 115 131Total (mL/kg*d) 70 95 126 144 159 152 137 150 146No supplementation other than vitamins and trace elements was necessary during theparenteral period

Fluid intakesFluid intake (ml/kg*d)1801601401201008060402001 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22Days of lifeEnteralParenteralTotal

Energy intakes160140Energy intake (kcal/kg*d)120100806040EnteralParenteralTotal2001 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22Days of life

Protein intakesProtein equivalent intake (g/kg*d)4,54,03,53,02,52,01,51,00,50,01 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22Days of lifeEnteralParenteralTotal

Weight gain25Weight gain (% of bith weight)20151050-51 2 3 4 5 6 7 8 9 10 11 12 13 14Days of life

Parenteral intakesD1 D2 D3 D4 D5 D6 D7 D8 D9AA (g/kg*d) 1.9 2.2 3.1 3.4 3.3 2.5 1.6 1.4 0.6Lipid (g/kg*d) 1.5 1.8 2.5 2.8 2.6 2.0 0.0 0.0 0.0Glucose (g/kg*d) 8.0 9.3 13.3 14.6 14.0 10.6 6.7 5.8 2.5Energy (kcal/kg*d) 55 63 85 94 89 68 31 27 12Na (mmol/kg*d) 1.3 1.5 2.1 2.3 2.2 1.7 1.1 0.9 0.4K (mmol/kg*d) 1.3 1.5 2.1 2.3 2.2 1.7 1.0 0.9 0.4Cl (mmol/kg*d) 1.9 2.2 3.1 3.4 3.3 2.5 1.6 1.4 0.6Ca (mg/kg*d) 31.2 36.4 52.0 57.2 54.6 41.6 25.6 22.4 9.6P (mg/kg*d) 24.0 28.0 40.0 44.0 42.0 32.0 16.0 14.0 6.0

BiologyD1 D2 D3 D4 D5 D6 D7 D8 D9 D10Creat (mg/dL) 0.77 0.81 0.57 0.50 0.45BUN (mg/dL) 10.0 12.0 6.7 4.7 2.7Gly (g/L) 1.00 0.72 1.10 1.43 1.8 1.28 0.7Na (mmol/L) 138.3 140.8 140.0 144.6 138.7 140.0K (mmol/L) 4.7 4.1 4.4 4.1 4.8 4.4Cl (mmol/L) 100.3 100.5 102.1 105.1 102.8 101.9BE (mmol/L) -3.0 -2.4 -3.8 -3.0 -1.1 1.4Ca (mg/dL) 9.5 10.7 11.0 10.7P (mg/dL) 4.9 5.7 5.6 8.3