HT DNA High Sensitivity LabChip Kit LabChip GX/GXII User Guide

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7. Symptom: Unexpected sharp peaks.HT DNA High Sensitivity LabChip ® KitLabChip GX/GXII User GuideThe electropherogram of a DNA High Sensitivity ladder is shown below with an unexpected sharp peakcaused by dust or other particulates.LowermarkerPeak caused byparticulatesUppermarkerPossible causes:A) Dust or other particulates introduced through sample or reagents.What to do:A) Replace molecular biology grade water used for chip preparation. Replace buffer used for sampleand reagent preparation. Use a 0.22-micron filter for all water and buffers used for chip, sample, andreagent preparation.Caliper Life Sciences Page 16 of 22 Revised Nov 201068 Elm StreetHopkinton, MA 01748-16681-877-LABCHIP (522-2447)
Frequently Asked QuestionsHT DNA High Sensitivity LabChip ® KitLabChip GX/GXII User Guide1. Why is 120 μL of gel-dye needed in Chip Well 10 for the HT DNA High Sensitivity assay and not theother HT DNA assays?As a result of the formulation of the HT DNA High Sensitivity assay additional ions are needed.2. Why is 140 μL of Marker solution needed to run 96 samples using the HT DNA High Sensitivityassay and only 50 μL of Marker solution for the other HT DNA assays?For the HT DNA High Sensitivity assay more marker and sample are sipped compared to the other HTDNA assays.3. If my DNA samples fall within the sizing range and linear concentration range of both the HT DNA1K and the HT DNA High Sensitivity assays, which assay should I use?The HT DNA High Sensitivity assay should be used since less sample is required to run this assay. If thesample exceeds the assay concentration limits of the HT DNA High Sensitivity assay, then it can befurther diluted and re-tested.4. Why can only 96 samples be tested per chip preparation for the HT DNA High Sensitivity assay andup to 384 samples for the other HT DNA assays?For the HT DNA High Sensitivity assay ion depletion occurs past 96 samples. Additionally since moremarker and sample are sipped compared to the other HT DNA assays the waste well in the chip fills upmore quickly.5. If using the HT DNA High Sensitivity assay to test samples fractionated by the LabChip XT, whatbuffer do I use to prepare the ladder and buffer for the Buffer Tube?To test LabChip XT fractionated samples use the Limited Sample Workflow. To prepare the ladder andbuffer for the Buffer Tube use LabChip XT Running Buffer as the DNA Sample Buffer. After removingextracted collection from LabChip XT chip, pipette out 300uL of Running Buffer from source well.Caliper Life Sciences Page 17 of 22 Revised Nov 201068 Elm StreetHopkinton, MA 01748-16681-877-LABCHIP (522-2447)
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HT DNA High Sensitivity LabChip Kit LabChip GX/GXII User Guide

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