Considerations When Optimizing Coating Protocols for Corning ...
Considerations When Optimizing Coating Protocols for Corning ...
Considerations When Optimizing Coating Protocols for Corning ...
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Figure 3. Neuro-2a Cells on Lamininn-Coated Transwell ® Permeable SupportsA.<strong>Coating</strong>Time<strong>Coating</strong>ConcentrationPBSSFM<strong>Coating</strong>Time<strong>Coating</strong>ConcentrationPBSSFM10 µg/cm 210 µg/cm 26 hours2 µg/cm 230minutes2 µg/cm 21 µg/cm 21 µg/cm 210 µg/cm 2Uncoated2 hours2 µg/cm 2B.SFM at RT SFM at 37°C10 µg/cm 2<strong>for</strong> 2 hours1 µg/cm 2<strong>Corning</strong> IncorporatedLife SciencesTower 2, 4th Floor900 Chelms<strong>for</strong>d St.Lowell, MA 01851t 800.492.1110t 978.442.2200f 978.442.2476www.corning.com/lifesciencesWorldwideSupport OfficesA S I A / PA C I F I CAustralia/New Zealandt 0402-794-347Chinat 86-21-5467-4666f 86-21-5407-5899Indiat 91 124 4604000f 91 124 4604099<strong>Corning</strong> and Transwell are registered trademarks of <strong>Corning</strong> Incorporated, <strong>Corning</strong>, NY.All other trademarks in this document are the property of their respective owners.<strong>Corning</strong> Incorporated, One Riverfront Plaza, <strong>Corning</strong>, NY 14831-0001Japant 81 3-3586 1996f 81 3-3586 1291Koreat 82 2-796-9500f 82 2-796-9300Singaporet 65 6733-6511f 65 6861-2913Taiwant 886 2-2716-0338f 886 2-2716-0339E U R O P EFrancet 0800 916 882f 0800 918 636Germanyt 0800 101 1153f 0800 101 2427The Netherlandst 31 20 655 79 28f 31 20 659 76 73United Kingdomt 0800 376 8660f 0800 279 1117All Other EuropeanCountriest 31 (0) 20 659 60 51f 31 (0) 20 659 76 73L AT I N A M E R I C ABrasilt (55-11) 3089-7419f (55-11) 3167-0700Mexicot (52-81) 8158-8400f (52-81) 8313-8589© 2010 <strong>Corning</strong> Incorporated Printed in USA 3/10 POD CLS-AN-134
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