Observing Microorganisms through a Microscope

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Bacteria are slightly negative, so are attractedto the positive chromophore of the BASICDYE• Common Basic Dyes• crystal violet• methylene blue• safranin• basic fuchsin
Acid Dyes - used for Negative Staining(background is stained)Mordant - intensifies the stain or coats a structure tomake it thicker and easier to see after it is stainedExample:Flagella - can not normally be seen, but a mordantcan be used to increase the diameter of the flagellabefore it is stainedSalmonella typhosa
Page 2: Basic techniques needed to studyBac
Page 9 and 10: Gram Stain• 1884 Hans Christian G
Page 11 and 12: Gram Stain2. Grams Iodine (mordant)
Page 13 and 14: Gram Stain4. Safranin (Counterstain
Page 15 and 16: Identification of a BacteriaUnknown
Page 17 and 18: 2 Important PathogensMycobacterium
Page 19 and 20: Acid - Fast Stain• 1. Carbolfuchs
Page 21 and 22: Flagella StainSpirillum volutans
Page 23: Clostridium botulinum

Observing Microorganisms through a Microscope

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