1,4-ï¬-D-Glucosyl-D-Mannobiose and 1,4-ï¬-D ... - Megazyme
1,4-ï¬-D-Glucosyl-D-Mannobiose and 1,4-ï¬-D ... - Megazyme
1,4-ï¬-D-Glucosyl-D-Mannobiose and 1,4-ï¬-D ... - Megazyme
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
1,4-ß-D-<strong>Glucosyl</strong>-D-<strong>Mannobiose</strong> <strong>and</strong> 1,4-ß-D-Cellobiosyl-D-Mannose(Lot 120603)O-GMMTR 06/12MW: 504.44CAS No. -1,4-β-D-Glc-1,4-β-D-Man-D-Man + 1,4-β-D-Glc-1,4-β-D-Glc-D-Man(~ 42%) (~ 50%)PREPARATION:PURITY:Prepared by controlled enzymic hydrolysis of konjac glucomannan.~ 92% (contaminants are the trisaccharides ~ 4% MGM <strong>and</strong> ~ 4% MMM)HPLC:HPLC was performed on a Waters Sugar-Pak ® Column (6 x 300 mm); temperature 90°C; mobilephase, distilled water containing 50 mg/L of disodium calcium EDTA (Sigma Cat No. ED2SC); flowrate 0.5 mL/min. A Breeze HPLC System was used incorporating Waters 2410 RI detector <strong>and</strong>Breeze Version 3.20 software.1
Thin layer chromatography of the trisaccharide fraction obtained on hydrolysis ofkonjac glucomannan by β-mannanase.(Characterisation of the trisaccharide mixture (A) by hydrolysis withβ-glucosidase <strong>and</strong> β-mannosidase).MannoseGlucose<strong>Mannobiose</strong>1,4-β-D-Man-1,4-β-D-Glu-D-ManCellobiose1,4-β-D-Cellobiosyl-D-mannose1,4-β-D-<strong>Glucosyl</strong>-1,4-β-D-mannobioseMannotrioseMannotrioseMannopentaoseA B C D EA. Trisaccharides from β-mannanase hydrolysis of konjac glucomannan.B. “A” incubated with β-glucosidase showing hydrolysis of Glc-Glc-Man <strong>and</strong> Glc-Man-Manto glucose + mannose <strong>and</strong> glucose plus mannobiose, respectively.C. “A” incubated with β-mannosidase showing no hydrolysis of Glc-Glc-Man orGlc-Man-Man, but hydrolysis of Man-Man-Man <strong>and</strong> Man-Glu-Man to glucose plusmannose.D. Mannose; 1,4-β-D-mannotriose <strong>and</strong> 1,4-β-D-mannopentaose.E. Glucose <strong>and</strong> cellobiose.2
Change language