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Program Book - The Master Brewers Association of the Americas

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O-25<br />

Cell wall modification: State <strong>of</strong> play<br />

Presenter: Charles Bamforth, University <strong>of</strong> California, Davis, CA<br />

We have come a long way from <strong>the</strong> pioneering work <strong>of</strong> <strong>the</strong> likes<br />

<strong>of</strong> Luchsinger and Preece in understanding <strong>the</strong> composition and<br />

degradation <strong>of</strong> <strong>the</strong> cell walls <strong>of</strong> <strong>the</strong> starchy endosperm <strong>of</strong> barley.<br />

Whereas <strong>the</strong> ins and outs <strong>of</strong> <strong>the</strong> heat-sensitive endo-barley-βglucanases<br />

are well-understood, we now appreciate <strong>the</strong> intimate<br />

role that enzymes such as carboxypeptidase, feruloyl esterase,<br />

acetylxylan esterase, arabanin<strong>of</strong>uranosidase, and endo-xylanases<br />

play in determining <strong>the</strong> breakdown <strong>of</strong> glucan in malting and mashing.<br />

This complex enzymology is reviewed in such a way as to<br />

highlight <strong>the</strong> practical realities <strong>of</strong> achieving homogeneous modification<br />

and <strong>the</strong> fewest brewhouse problems.<br />

Charlie Bamforth is <strong>the</strong> Anheuser-Busch Endowed Pr<strong>of</strong>essor<br />

<strong>of</strong> Malting & Brewing Sciences at <strong>the</strong> University <strong>of</strong> California,<br />

Davis. He has been part <strong>of</strong> <strong>the</strong> brewing industry for 31 years.<br />

He is <strong>the</strong> former deputy director-general <strong>of</strong> Brewing Research<br />

International and research manager and quality assurance manager<br />

<strong>of</strong> Bass <strong>Brewers</strong>. He is a special pr<strong>of</strong>essor in <strong>the</strong> School <strong>of</strong><br />

Biosciences at <strong>the</strong> University <strong>of</strong> Nottingham, England, and was<br />

previously visiting pr<strong>of</strong>essor <strong>of</strong> brewing at Heriot-Watt University<br />

in Scotland. Charlie is a Fellow <strong>of</strong> <strong>the</strong> Institute <strong>of</strong> Brewing &<br />

Distilling, <strong>the</strong> Institute <strong>of</strong> Biology, and <strong>the</strong> International Academy<br />

<strong>of</strong> Food Science and Technology. Charlie is editor-in-chief <strong>of</strong> <strong>the</strong><br />

Journal <strong>of</strong> <strong>the</strong> American Society <strong>of</strong> Brewing Chemists and a member<br />

<strong>of</strong> <strong>the</strong> editorial boards <strong>of</strong> several o<strong>the</strong>r journals, including <strong>the</strong><br />

MBAA Technical Quarterly. Charlie has published innumerable<br />

papers, articles, and books on beer and brewing, most recently<br />

editing Beer: A Quality Perspective (Academic Press). For 15<br />

years in his native England, he wrote about soccer and has been<br />

published extensively in match-day programs and magazines. He<br />

also authored a book about goalkeepers. Charlie has been married<br />

to Diane for 32 years and <strong>the</strong>y have three children and one<br />

grandchild.<br />

O-26<br />

Bioactive compounds in <strong>the</strong> malting process<br />

Presenter: Moritz Krahl, TU München<br />

Coauthor(s): Thomas Becker, TU München<br />

Nowadays human nutrition is mainly based on only three cereals,<br />

wheat, rice and corn, which contribute over 75% to <strong>the</strong> world’s<br />

starch production. At <strong>the</strong> same time, diseases caused by wrong or<br />

unbalanced diets are causing severe problems in Western countries.<br />

In this regard, <strong>the</strong> enrichment <strong>of</strong> <strong>the</strong> malting process with<br />

bioactive compounds, with <strong>the</strong> objective <strong>of</strong> providing beneficial<br />

health effects to <strong>the</strong> consumer, is a very important field. <strong>The</strong> malting<br />

process is influenced by <strong>the</strong> quality <strong>of</strong> <strong>the</strong> raw material and<br />

several process parameters (e.g., moisture, temperature, and time),<br />

and <strong>the</strong> enrichment <strong>of</strong> bioactive compounds depends on <strong>the</strong> same<br />

variables. A statistical design <strong>of</strong> experiments (DOE) approach<br />

(response surface methodology) was used to evaluate <strong>the</strong> interactions<br />

<strong>of</strong> <strong>the</strong> influential factors and optimize <strong>the</strong> enrichment process.<br />

One group <strong>of</strong> <strong>the</strong> functional components we investigated is<br />

<strong>the</strong> arabinoxylans. In order to investigate this group <strong>of</strong> substances,<br />

it was necessary to establish a method for determining watersoluble<br />

and water-insoluble arabinoxylans. <strong>The</strong> method we used<br />

consists <strong>of</strong> acidic hydrolysis <strong>of</strong> <strong>the</strong> arabinoxylans, followed by<br />

HPAEC/PAD detection. Using this method, we were able to enrich<br />

water-soluble arabinoxylans in wheat malt. Ano<strong>the</strong>r interesting<br />

group <strong>of</strong> components is <strong>the</strong> flavonols. We established a method for<br />

<strong>the</strong> characterization <strong>of</strong> flavonols like rutin, vitexin, and quercetin<br />

by HPLC separation. This method has helped us to determine optimum<br />

malting parameters for <strong>the</strong> enrichment <strong>of</strong> <strong>the</strong>se functional<br />

components in buckwheat malt. Additionally, changes in <strong>the</strong> vitamin<br />

B 1 and B 2 content <strong>of</strong> cereals were monitored during <strong>the</strong> whole<br />

germination and malting process <strong>of</strong> different cereals, as well as <strong>the</strong><br />

changes in water-soluble arabinoxylan and fructan.<br />

Moritz Krahl was born in 1980 in Schwetzingen, Germany. After<br />

attaining <strong>the</strong> German Abitur (A-level certificate) in 2000, he<br />

began studying brewing and beverage technology at <strong>the</strong> Technical<br />

University <strong>of</strong> Munich, Weihenstephan. In 2005 he graduated<br />

with a Dipl.-Ing. degree and has since <strong>the</strong>n been working as a<br />

doctoral student at <strong>the</strong> Chair for Technology <strong>of</strong> Brewing I in<br />

Weihenstephan.<br />

29

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