B - Plant Physiology

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Table V. Redistribution of radiolabel after incubation with [U 14 C]glucose in tuber discs of B33-TPS and B33-TPP lines. Tubers discs were cut from growing tuber of six separate plants per genotype. After 4 h of incubation, the tuber discs were extracted and fractionated. Values represent the mean ± SD of measurements of six individual plants per genotype. Values in bold denote fractions further fractionated into subfractions. Values in italics show significant subfractions of the soluble fraction. * denotes significant difference (P
Total metabolised Becq/gFW 14 C-incorporation WT B33-TPS16 B33-TPS17 B33-TPS29 1468.4 ± 111.7 1649.6 ±330.0 1460.3 ± 329.0 1431.6 ± 176.8 Insoluble % 30.8 ± 6.1 38.1 ± 7.1 22.7 ± 5.8 36.9 ± 10.1 Protein % 1.8 ± 0.5 2.7 ± 0.7 3.2 ± 1.3 2.9 ± 0.7 Starch % 17.9 ± 6.8 22.3 ± 6.9 9.5 ± 5.5 20.1 ± 8.6 Cellwall % 11.1 ± 1.4 13.1 ± 2.9 10.0 ± 3.1 14.0 ± 4.3 Soluble % 69.2 ± 6.1 61.9 ± 7.1 77.3 ± 5.8 63.1 ± 10.1 Amino acid % 3.8 ± 0.7 4.3 ± 0.9 4.2 ± 1.2 3.8 ± 0.3 Sugars % 57.5 ± 7.4 46.2 ± 8.1 59.8 ± 5.7 45.1 ± 9.8 Acidic % 7.9 ± 1.2 11.3 ± 1.6 * 13.2 ± 5.4 14.1 ± 1.3 * Organic acids % 5.4 ± 1.0 8.9 ± 1.1 * 10.1 ± 4.1 * 10.7 ± 1.2 * Released CO2 % 2.7 ± 0.2 3.6 ± 0.7 * 4.0 ± 0.8 * 4.0 ± 0.5 * Total metabolised Becq/gFW 14 C-incorporation WT B33-TPP11 B33-TPP26 B33-TPS34 631.8 ± 179.1 733 ± 111.1 666.2 ± 188.9 445.9 ± 55.5 Insoluble % 46.3 ± 21.5 48.1 ± 19.7 39.1 ± 13.6 36.9 ± 8.5 Protein % 2.2 ± 0.8 1.9 ± 0.9 1.9 ± 0.5 1.9 ± 1.2 Starch % 32.7 ± 16.6 33.9 ± 14.7 27.2 ± 12.1 25.5 ± 6.5 Cellwall % 11.4 ± 4.8 12.5 ± 4.9 10.0 ± 2.1 9.5 ± 2.9 Soluble % 37.2 ± 10.0 37.8 ± 13.7 34.6 ± 9.4 44.1 ± 24.5 Amino acid % 12.9 ± 6.8 13.0 ± 3.2 15.3 ± 2.2 11.9 ± 3.6 Acidic % 12.2 ± 6.0 9.5 ± 5.5 6.7 ± 5.9 11.2 ± 6.2 Organic acids % 11.7 ± 1.0 12.1 ± 1.2 10.9 ± 0.4 11.1 ± 0.7 Released CO2 % 3.2 ± 0.9 2.4 ± 0.3 3.0 ± 1.1 3.4 ± 1.1 Sucrose % 6.7 ± 1.3 23.6 ± 6.3 * 17.6 ± 4.4 * 25.6 ± 1.8 * Other Sugars % 6.3 ± 1.5 9.0 ± 3.7 * 9.8 ± 1.0 * 5.2 ± 1.9 47
Page 1 and 2: Plant Physiology Preview. Published
Page 3 and 4: This work was supported by a grant
Page 5 and 6: Introduction Cellular homeostasis c
Page 7 and 8: with the reduction in T6P content (
Page 9 and 10: In B33-TPS lines glucose levels wer
Page 11 and 12: plastids is differentially affected
Page 13 and 14: Inhibition of potato tuber SnRK1 ac
Page 15 and 16: (Hartmann et al., 2011), clear sign
Page 17 and 18: Uptake and utilization of exogenous
Page 19 and 20: involved in biosynthetic processes
Page 21 and 22: Mutants or transgenic plants with a
Page 23 and 24: Arabidopsis, pea and tomato (Bradfo
Page 25 and 26: Tubers of about 20 g fresh weight,
Page 27 and 28: Determination of metabolites using
Page 29 and 30: For q-RT-PCR analysis RNA was isola
Page 31 and 32: Supplemental Table S2: Overlap betw
Page 33 and 34: Eastmond PJ, van Dijken AJH, Spielm
Page 35 and 36: Lunn JE, Feil R, Hendriks JH, Gibon
Page 37 and 38: Schluepmann H, Pellny T, van Dijken
Page 39 and 40: Figure legends Figure 1: Reverse tr
Page 41 and 42: compared to wildtype tubers in spro
Page 43 and 44: Table II. Modulation of the T6P con
Page 45: Table IV. Levels of hexose-phosphat
Page 49 and 50: Figure 1. Figure 1: Reverse transcr
Page 51 and 52: Down-regulated Up-regulated 35 30 5
Page 53 and 54: A B nmol phosphate incorporated int
Page 55 and 56: elative expression 10 1 0.1 Sucrose
Page 57 and 58: % of meristems with visible sprout

B - Plant Physiology

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