B - Plant Physiology

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Table VI. Steady state levels of adenine nucleotides in B33-TPS and B33-TPP transgenic tubers compared to the wild type control. Values represent mean (±SD) of 10 individual plants. *denotes significant difference (P < 0.001) according to Student’s t test (II) compared to the wild type control. n mol g -1 FW ATP ADP ATP/ADP ratio WT 21.2 ± 2.4 3.0 ± 0.4 7.2 ± 0.9 B33-TPS 16 6.7 ± 0.9 * 4.7 ± 0.6 * 1.4 ± 0.1 * B33-TPS 17 13.9 ± 0.9 * 6.1 ± 0.3 * 2.3 ± 0.2 * B33-TPS 29 4.9 ± 0.3 * 8.3 ± 1.2 * 0.6 ± 0.1 * B33-TPP 11 20.2 ± 1.0 7.5 ± 0.4 * 2.7 ± 0.2 * B33-TPP 26 37.7 ± 1.7 * 24.1 ± 0.9 * 1.6 ± 0.1 * B33-TPP 34 46.9 ± 2.2 * 14.3 ± 0.8 * 3.3 ± 0.3 * 48
Figure 1. Figure 1: Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of transgene expression in tubers of selected transgenic potato lines. Ubiquitin served as an internal standard to ensure that equal amounts of cDNA were used for the reactions.
Page 1 and 2: Plant Physiology Preview. Published
Page 3 and 4: This work was supported by a grant
Page 5 and 6: Introduction Cellular homeostasis c
Page 7 and 8: with the reduction in T6P content (
Page 9 and 10: In B33-TPS lines glucose levels wer
Page 11 and 12: plastids is differentially affected
Page 13 and 14: Inhibition of potato tuber SnRK1 ac
Page 15 and 16: (Hartmann et al., 2011), clear sign
Page 17 and 18: Uptake and utilization of exogenous
Page 19 and 20: involved in biosynthetic processes
Page 21 and 22: Mutants or transgenic plants with a
Page 23 and 24: Arabidopsis, pea and tomato (Bradfo
Page 25 and 26: Tubers of about 20 g fresh weight,
Page 27 and 28: Determination of metabolites using
Page 29 and 30: For q-RT-PCR analysis RNA was isola
Page 31 and 32: Supplemental Table S2: Overlap betw
Page 33 and 34: Eastmond PJ, van Dijken AJH, Spielm
Page 35 and 36: Lunn JE, Feil R, Hendriks JH, Gibon
Page 37 and 38: Schluepmann H, Pellny T, van Dijken
Page 39 and 40: Figure legends Figure 1: Reverse tr
Page 41 and 42: compared to wildtype tubers in spro
Page 43 and 44: Table II. Modulation of the T6P con
Page 45 and 46: Table IV. Levels of hexose-phosphat
Page 47: Total metabolised Becq/gFW 14 C-inc
Page 51 and 52: Down-regulated Up-regulated 35 30 5
Page 53 and 54: A B nmol phosphate incorporated int
Page 55 and 56: elative expression 10 1 0.1 Sucrose
Page 57 and 58: % of meristems with visible sprout

B - Plant Physiology

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