Live cell imaging - Biomedicum Imaging Unit (BIU) - Helsinki.fi

Fluorescent resonance energy transfer (FRET)• 3D colocalization –optical resolution 250 nm is a limitation• FRET –an extension to the resolution of optical microscopy• Acceptor bleaching or sensitized emission• Ultimate protein colocalization and interactions at at molecular level• Not trivial if quantification required –rigorous controls and optimizationExcitation< 10 nmEmissionDonorAcceptorEmissionEnergy transfer without emitted fluorescenceFluorescence imaging 1