Live cell imaging - Biomedicum Imaging Unit (BIU) - Helsinki.fi
Live cell imaging - Biomedicum Imaging Unit (BIU) - Helsinki.fi
Live cell imaging - Biomedicum Imaging Unit (BIU) - Helsinki.fi
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Fluorochromes, spectral pro<strong>fi</strong>les and <strong>fi</strong>lters• The larger the Stokes shift, the better the spectralresolution utilized in molecular analyses• Spectral differences allow simultaneous detectionof multiple fluorochromes• Detection of fluorescence requires means of<strong>fi</strong>ltering the spectral components –<strong>fi</strong>lters, slitgratings, AOBS• Typical fluorescence <strong>fi</strong>lter block consists of– Excitation <strong>fi</strong>lter– Dichromatic <strong>fi</strong>lter/ mirror– Emission <strong>fi</strong>lterFluorescence <strong>imaging</strong> 1
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