The Structure and Function of Enzymes MODULE 1 MODULE 2 ...

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Should You Put Fresh or Canned Pineapple in Gelatin?Module 1 Exploration ActivityAdapted by George Morse from an activity created by Leslie Greenberg, Einstein High SchoolBackgroundThe pineapple, Ananas comosus, belongs to the bromeliad family. Pineapple fruitcontains the protein-degrading enzyme bromelain. Unlike fresh pineapple, cannedpineapple is heated to very high temperatures during the canning process in order to killbacteria.ProblemDoes gelatin contain protein?HypothesisIf gelatin contains protein, then treatment with a protein-degrading enzyme should alterthe gelatin.Procedure• Examine the plain gelatin and record your observations in the table below.• Take a small sample of plain gelatin in a petri dish.• Liberally sprinkle meat tenderizer on the gelatin sample and let it sit for five minutes.Continue to the next step of the procedure.• Examine the gelatin made with fresh pineapple and the gelatin made with cannedpineapple. Record your observations in the table below.• Examine the gelatin sprinkled with meat tenderizer and record your observations inthe table below.DataTable 1: Observations recorded during the experiment.Type of GelatinPlain Gelatin (Gelatin + Water)ObservationsPlain Gelatin + Fresh PineapplePlain Gelatin + Canned PineapplePlain Gelatin + Meat TenderizerQuestions1. Does pineapple contain an enzyme that digests protein? Support your answer withobservations made during the laboratory investigation.2. How do your observations of gelatin + fresh pineapple differ from your observationsof gelatin + cooked pineapple? Explain why they differ.3. What can you infer about the ingredients in meat tenderizer?14
What are Enzymes Made Of?Module 1 Explanation ActivityThe post-lab activity is the explanation phase of the module.Go over the questions that were posed in the exploration activity (Should You Put Freshor Canned Pineapple in Gelatin?). This is a good time to distinguish between inferencesand observations. The concept of qualitative testing should be identified – if a changeoccurs, it is a positive test result. If not, it is a negative result. Students frequently hearabout this type of testing (he tested positive for strep throat, for example) but do not havea practical understanding of what it means.Question 1Does pineapple contain an enzyme that digests protein? Yes [Note: Some students maystate that the enzyme is only in fresh pineapple.]Support your answer with observations made during the laboratory investigation. Theplain gelatin that had fresh pineapple in it did not solidify. The plain gelatin(without pineapple) did solidify. [This is a good time to identify the control &variable treatments in a lab activity.]Question 2How do your observations of gelatin + fresh pineapple differ from your observations ofgelatin + cooked pineapple? The gelatin + fresh pineapple did not solidify while thegelatin + cooked pineapple did solidify.Explain why they differ. The heat denatured the enzyme in the pineapple. [This is agood time to discuss the effects of heat on proteins if it has not been covered before.]Question 3What can you infer about the ingredients in meat tenderizer? Meat tenderizer contains aprotease. [The ase suffix denotes an enzyme; students can be asked to defend theiranswers based on information obtained in class from the previous answers.]ClosureReturn to the list of items that were developed by the students during the brainstormingactivity. Confirm or reject items that were dealt with by the lab. The explanation activitycan be closed with a summary of the structural properties of an enzyme.15
Page 1 and 2: The Structure andFunction of Enzyme
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Page 5 and 6: MODULE #1Module Title: What Are Enz
Page 7 and 8: MODULE #2Module Title: What Do Enzy
Page 9 and 10: MODULE #3Module Title: What Affects
Page 11 and 12: Goal 3 The student will demonstrate
Page 13: What are Enzymes Made Of?Module 1 E
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Page 19 and 20: Closure for the ActivityThere are t
Page 21 and 22: Table 1: This is a data table for r
Page 23 and 24: What Do Enzymes Do and How Do They
Page 25 and 26: What Affects the Function of an Enz
Page 27 and 28: The Effects of pH on the Function o
Page 29 and 30: Conclusions:1. State the pH range i
Page 31 and 32: 4. Dr. T.H. Oliver reported the fir
Page 33 and 34: What Affects the Function of an Enz
Page 35: HSA Rubric for Constructed-Response

The Structure and Function of Enzymes MODULE 1 MODULE 2 ...

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