Research Models - Constant Systems Limited

Cell Disruption it’s in our GenesCell Disruption SolutionsConstant Systems Research ModelsTaking your disruption out of Cell Disruptioncell disruptionprotein extractionenzyme releasecontinuous processingDNA manipulationsingle cell isolationtissue disruptionselective breakagewww.constantsystems.comRev 0FS67550ISO 9001:2000 Registered

Principles ofOperationConstant Cell Disruption Systems are based on the use of highpressure to force a sample through a small fixed orifice at highspeed under controlled, contained and repeatable conditions.1. As the high pressurepiston descends,sample is introducedinto the high pressurecylinder.2. The high pressure piston thenaccelerates the sample through the fixedorifice at high velocity. The controlledacceleration causes the cells to rupture.3. When the jet containing disrupted cells hits the target,it is spread radially across the cooled heat exchangesurface. Product then flows from the disruptionchamber for collection. The hydraulics are rechargedand the cycle continues.Reproducible ResultsConstant Cell Disruption Systems give reproducible and superior resultsbecause control of the disruption pressure is achieved by the hydrauliccontrol system.The disruption pressurecycle is shownadjacent. This has beencreated by analysingand recording thehydraulic pressure inthe operating cylinder,which is related to thedisruption pressure bythe ratio of the pistonareas.Pressure (bar x 100)302010DisruptionstrokeReturnstrokeRecharging periodfor hydraulics22 4 6 8 10 12 14Time (second)Graph showing a typical controlled pressure cycle in the cell disrupter40302010Pressure (psi x 1000)Contained ProcessThe disruption head, high pressure cylinder and cooling system have beendesigned with containment and safety in mind so that the volume of highpressure product is kept to minimum at all times. The integrity of thecontainment system has been tested by the Biosafety Testing Station at theCentre for Applied Microbiology Research, UK. “ ...a high level ofbio-containment.” “No evidence of emission from the equipment in any test.”

Cell Disruption SolutionsOne Shot ModelThe One Shot model is the ideal research tool for small processing.It can process solid samples, cell paste and suspended samplesquickly and efficiently. Work and results can be scaled upthroughout the range.Specifications shown over leafSpecifications shown overleafPower RatingVolume per Shot vs Maximum Pressure40 KPSI (2700 bar) 30 KPSI (2000 bar) 20 KPSI (1350 bar) 15 KPSI (1000 bar)0.75 KW 1-8ml 1.5-10.5ml 2-16ml 3-20.5mlwww.constantsystems.com

Cell Disruption SolutionsOne Shot ModelIncorporates our unique disruption head and precise hydraulic operating and controlsystemQuick and easy to useDisruption pressure easily and accurately set and displayed digitally with a range of1KPSI through to 40KPSI as standardUltra low lossContained during operationPressure consistent and stable during disruption cycleDisrupter head mounted in stainless steel tray to avoid spillageEasily dismantled for manual cleaning and autoclavingAllows loading of solid sample, cell paste or suspended sampleCollection cups provided can be transferred to and from ice or a freezer forMaterials in contact with sample, 316L and F51 duplex stainless steel or equivalent,synthetic sapphire, EPDM, GLFPTFE & PEEK 450G475450Constant Systems Limited, Low March, Daventry, Northants, NN11 4SD, United KingdomTelephone: + 44 (0) 1327 314146 Fax : + 44 (0) 1327 314147

Cell Disruption SolutionsTS Series 0.75kw ModelThe TS 0.75kw model is the ideal research/pilot tool for processingbetween 40mls and 200mls and is versatile to adapt as your needschange.Specifications shown overleafPower RatingFlow Rates vs Maximum Pressure40 KPSI (2700 bar) 30 KPSI (2000 bar) 20 KPSI (1350 bar) 15 KPSI (1000 bar)0.75 KW 40ml/min 55ml/min 80ml/min 115ml/minwww.constantsystems.com

Cell Disruption SolutionsTS Series 0.75kw ModelIncorporates our unique disruption head and precise hydraulic operating controlsystem Disruption pressure easily and accurately set and displayed digitally with arange of 1KPSI through to 40KPSI as standardTouch screen control of disruption cycle, automatic shut down when processcompleteDisruption head mounted on tray to contain accidental spillagesPartial containment and simple externally controlled CIPInternal relief valve fitted for reverse flow of cleaning fluidsVarious flow rates are available depending on desired maximum pressure and powerrating, alternative pressure heads available on requestPressure consistent and stable during disruption cycleFull bio-containment inlet and outlet options available upon requestMaterials in contact with sample, 316L and F51 duplex stainless steel or equivalent,synthetic sapphire, EPDM, GLFPTFE & PEEK 450GTemperature Control: Disruption head is surrounded by a unique cooling jacket. Bathcirculator or chiller is recommended.750690Constant Systems Limited, Low March, Daventry, Northants, NN11 4SD, United KingdomTelephone: + 44 (0) 1327 314146 Fax : + 44 (0) 1327 314147

Cell Disruption SolutionsTS Series CabinetTS Series Cabinets are the ideal pilot production models and areversatile enough to adapt as your needs change.Power RatingFlow Rates vs Maximum Pressure40 KPSI (2700 bar) 30 KPSI (2000 bar) 20 KPSI (1350 bar) 15 KPSI (1000 bar)2.2 KW190ml/min240ml/min300ml/min310ml/min4 KW405ml/min430ml/min440ml/min565ml/minwww.constantsystems.com

Cell Disruption SolutionsTS Series CabinetIncorporates our unique disruption head and precise hydraulic operating controlsystemDisruption pressure easily and accurately set and displayed digitally with a range of1KPSI through to 40KPSI as standardTouch screen control of disruption cycle, automatic shut down when processcompleteCell Disrupter controls peristaltic process pump for automatic supply of largersamples.Process pump available on requestDisruption head mounted in stainless steel tray to avoid spillagePartial containment and simple externally controlled CIPInternal relief valve fitted for reverse flow of cleaning fluidsVarious flow rates are available depending on desired maximum pressure and powerrating, alternative pressure heads available on requestPressure consistent and stable during disruption cycleFull bio-containment inlet and outlet options available on requestMaterials in contact with sample, 316L and F51 duplex stainless steel or equivalent,Temperature synthetic Control: sapphire, Disruption EPDM, GLFPTFE head is surrounded & PEEK 450G by a unique cooling jacket . Bathcirculator IQ/OQ or available chiller is recommended.on requestConstant Systems Limited, Low March, Daventry, Northants, NN11 4SD, United KingdomTelephone: + 44 (0) 1327 314146 Fax : + 44 (0) 1327 314147

Cell Disruption SolutionsB & C SeriesThe B & C Series are the ultimate industry cell disrupters, with thepower and processing ability to consistently disrupt the materials thatyou require.Power RatingFlow Rates vs Maximum Pressure40 KPSI (2700 bar) 30 KPSI (2000 bar) 20 KPSI (1350 bar) 15 KPSI (1000 bar)1.1 KW2.2 KW4 KW100ml/min 120ml/min 180ml/min 255ml/min190ml/min 240ml/min 300ml/min 310ml/min405ml/min 430ml/min 440ml/min 565ml/minwww.constantsystems.com

Cell Disruption SolutionsB & C SeriesIncorporates our unique disruption head and precise hydraulic operating controlsystemwith CIP and SIP capabilityDisruption pressure easily and accurately set and displayed digitally with a range of1KPSI through to 40KPSI as standardCell Disrupter controls peristaltic process pump for automatic supply of largersamples.Process pump available on requestFully contained and having internal ports and drain ports to allow CIP and SIPThe “B Series” allows the CIP and SIP to be manually operatedThe “C Series” includes all valves which can be tailored to customers requirementsand validatedMaterials in contact with sample, 316L and F51 duplex stainless steel or equivalent,synthetic sapphire, EPDM, GLFPTFE & PEEK 450GIQ/OQ available on requestVarious flow rates are available depending on maximum pressure and power rating,alternative pressure heads available on requestPressure consistent and stable during disruption cycleValidation: Industry standard validations available on request785690*B Series Bench Top model shownabove and B series Cabinet modelshown on the reverseConstant System Limited, Low March, Daventry, Northants, NN11 4SD, United KingdomTelephone: + 44 (0) 1327 314146 Fax : + 44 (0) 1327 314147

Your Application and Cell SuccessConstant Cell Disrupter Systems are extremely versatile and have been used for the following rangeof application:Cell Disruption, Protein & DNA Extraction, Enzyme Release, DNA Manipulation,Selective Breakage, Single Cell Isolation, Tissue DisruptionIndividual Organisms and samples processed by Constant Cell Disrupter Systems are too numerous tomention them all, however the following lists examples:AlgaeAnimal CellsBacteriaFungi(inc Yeasts)Disruption of anabaena variableSelective breakage of chicken spermGood enzyme activity from recombinant e.coli. High protein recovery frommycobacterium smegmatisHigh specific activity of enzyme from aspergillus nidulans. 99% protein releasefrom saccharomyces cerevisiae. 99% Recombinant protein release from pichiapastorisMammalianCells and TissueParasitesPlant Cells& TissueVirusesEnvironmentalSamplesDisruption of cellular and intracellular plasma membranes from horse liver Isolationof viable single cells from calf spleen and lymph nodeBreakage of cytomegalovirus in nucleus of human cellsPectin extraction from banana fruit. Strawberry leaf DNA extractionIsolation of membrane antigens from toxoplasma gondi. Separation of nucleusand membrane in amniotic cellsExtraction of DNA from soil bacteriaE.coli Disruption & Guidance ListConstant Systems Cell Disrupters experience withE.coli is vast and breakage depends on the cellculture conditions, length of growth cycle andpressure utilised.We state the following as a guide to users forpressure compared to breakage with E.coli:Release of DNA –Below 15,000 psi (1000 Bar)Cytoplasmic Protein –15,000 psi – 20,000 psi (1350 Bar)Inclusion Bodies –20,000 psi – 27,000 psi (1900 Bar)Membrane Protein –27,000 psi – 35,000 psi (2400 Bar)Yeast Disruption GuidePlease find below a list of some of the other more commonspecies of yeast with pressure vs. breakage:·Candida Cloacae·Candida Albicans·Candida Utilis·Hansenula Polymorpha·Pichia Augusta·Pichia Capsulatus·Pichia Picorna·Pichia Pastoris·Kluyveromyces Fragilis·Rhodotorula Glutinis·Schizosaccharomyces Pombe·Saccharomyces Cerevisiae- 40 Kpsi- 25 - 40 Kpsi- 15 - 40 Kpsi- 25 - 40 Kpsi- 40 Kpsi- 40 Kpsi- 25 - 35 Kpsi- 35 - 40 Kpsi- 40 Kpsi- 40 Kpsi- 15 - 40 Kpsi- 27 - 35 Kpsi