CONFIDENTIAL REPORT - Plant Research International

SECTION II: PROJECT PROGRESS REPORT1. OVERVIEW OF PROGRESS DURING THE REPORTING PERIOD1.1 Summary of the main objectives of the projectTable 1. List of the major workpackages and subdivision.WPno.Title ParticipantResp. InvolvedMM Start End Deliverables1 Production in plants 2 5 60 0 361.1 Constructs/transformation2 5 0 36 D6, 12, 14, 16, 17, 19, 21, 261.2 Bulk production 2 0 30 D2, 4, 5, 18, 272 Incorporation into feed 3 2, 5 8 12 36 D8, 9, 24, 283 Uptake and immuneresponses1,4 5, 2 78 0 363.1 In carp 1 5, 2 65 0 36 D7, 11, 15, 22, 23, 293.2 In rainbow trout 4 5, 2 48 0 36 D3, 11, 15, 22, 23, 294 Vaccination trials 5 1, 4, 5sub 53 0 364.1 Reference antigen/vaccine products5 5sub 0 24 D1, 4, 10, 204.2 Test in feed preparations 5 4, 5sub 18 36 D22, 23, 29, 30TOTAL 234As shown in Table 1, WP2 and WP4.2 did not start in the first year and hence data onthese WP’s cannot be given in this annual report.1.2 Overview of scientific progress of the project as a wholeProduction in plantsTubers harbouring recLTB and in addition, tubers harbouring LTB-fusion proteins thatare ideal for studying antigen transport, binding and uptake have been produced andmade available to partners 1 and 4 for studies in carp and rainbow trout, respectively.Two gene constructs coding for intact VHSV G protein and derivative lackingtransmembrane fragment, have been made and introduced in potato. Plants are currentlyin the greenhouse and setting tubers. Gene constructs coding for fusions of LTB andthese two VHSV G proteins have been prepared and are being introduced in potato by5