Sutter Instrument 2011 Catalog - Tsimpos.gr

PRICESPrices included herein are for items sold in theUSA or Canada (exclusive of transportation,insurance, and applicable taxes), and are subjectto change without notice. International pricesare 5% higher. Please contact Sutter Instrumentor your local representative for an overseasprice quotation.SHIPPINGShipping charges are prepaid and addedto our invoice. Unless otherwise specifiedwhen placing your order, we will use our bestjudgement in selecting a reliable and economicalshipper of our choice.RETURNSItems ordered in error may be returned within30 days of receipt, and are subject to a 15%restocking fee. You are urged to retain theoriginal shipping containers should there be aneed to return the item. Please contact SutterInstrument for a return authorization number.QUALITY CONTROLSutter Instrument takes great pride in meetingthe highest possible standards of qualityand reliability. Each instrument undergoesrigorous electronic and mechanical testingprotocols during the production process. Forour micropipette pullers, every instrumentis tested to assure its ability to consistentlyfabricate micropipettes with ultra-fi ne tips. Aseries of pipettes is pulled on each instrumentand examined with our scanning electronmicroscope. No other manufacturer offers thislevel of quality control.SERVICE & SUPPORTWe hope that our instruments and productscontinually meet your needs. However, shoulda problem arise, please contact our technicalsupport staff to discuss the problem. If theinstrument requires factory service, we willfurnish shipping instructions. Items underwarranty will be repaired free of any costs,for parts or service. Both delivery and returnshipping costs are the responsibility of theowner.WARRANTY INFORMATIONSutter Instrument provides a limited warrantyfor two years from shipping date on all Suttermade products and labor (except consumablesand Uniblitz ® shutters). To be covered underwarranty, the instrument must have beenoperated in accordance with the instructionsoutlined in the instruction manual and in amanner that would be expected in the normaluse of the product. Extended warranties maybe purchased for an additional charge. Pleasecontact Sutter for a quotation. Abuse, misuse, orunauthorized repairs will void any warranty.PAYMENT METHODSPayments may be made in U.S. Dollars in one ofthe following methods:Major Credit Card:Master Card and VISABank Draft drawn on a U.S bankInternational Money OrderBank Wire TransferIrrevocable Letter of Credit(a processing fee will apply)

INDEXMICROPIPETTE FABRICATIONIntroduction 4PULLERSP-2000 6Laser-Based Micropipette PullerNEW P-1000 10Next Generation Micropipette PullerP-97 Flaming/Brown Micropipette Puller 14P-30 Vertical Puller 18Micropipette Puller Comparison Chart 21Filaments/Accessories 22BEVELERBV-10 Microelectrode Beveler 24PRESSURE INJECTORManual Microinjector 28BOOKSAdvanced Techniques in 30Micropipette FabricationGLASSGlass Capillary Tubing 32MICROMANIPULATIONIntroduction 38MANIPULATORSMPC-200/MPC-385/MPC-325 40Multi-manipulator SystemMP-285 46Robotic MicromanipulatorMP-265/MPC-365 52Narrow Format ManipulatorMP-225 56Motorized MicromanipulatorMicromanipulator Comparison Chart 59MP-85 60Huxley-Wall Style MicromanipulatorMM-33 64MicromanipulatorTRANSLATORS, STAGES &MOUNTING SYSTEMS3DMS 683-Dimensional Motorized StageMT-2000 72Motorized X-Y Translation SystemMT-1000/MT-1078 78X-Y Translation SystemMP-78/MPC-78 84Large Moving Stage PlatformMT-78-FS 88Fixed Large PlatformStage & Translator Comparison Chart 93MT-70/MT-71 94StandsMT-75 98GantryMD SERIESGantry Style Platforms 104

OPTICAL INSTRUMENTSIntroduction 108MICROSCOPENEW MOM 110Movable Objective MicroscopeWAVELENGTH SWITCHERLambda DG-4/DG-5 PLUS 114LIGHT SOURCELambda XL 120Extended Life Light SourceLambda LS 124Xenon Light SourceFILTER WHEEL SYSTEMSLambda 10-3 130Lambda 10-2 136Lambda 10-B 142Filter Wheel Controller Comparison Chart 147MICROINJECTIONIntroduction 164XenoWorks ® Micromanipulator 166XenoWorks Digital Microinjector 170XenoWorks Analog Microinjector 174PrimeTech PMM-150FU 178Applications 180CUSTOM PRODUCTSOEM Products 186DEALERSInternational Dealer Directory 188SHUTTERINGLambda SC 148SmartShutter TM 152MICROSCOPE ADAPTERSAdapter list 156

MICROPIPETTE FABRICATIONGlass micropipettes are preciselyconstructed microtools forming the basisof a myriad of scientific investigativetechniques. Yet, the simplicity of a pieceof glass tubing belies the complexity ofthe technique that is required to producerepeatedly and accurately the micropipettecharacteristics a researcher desires.Sutter Instrument has endeavored formore than 30 years to produce the mostsophisticated instruments available forfabricating glass micropipettes. Ourexpertise in micropipette fabricationtechniques has been utilized by countlessnumbers of individuals who have appliedour knowledge to their particular research.The P-1000 is the latest evolution of theFlaming/Brown style puller. This pullerextends the features of the P-97 byincorporating a color touch screen andintegrating new technology that simplifiesprogramming. Unique features such asthe safe heat mode, diagnostic testing,line repeat mode and jaw temperaturesensor assist the user in creating the ideal4 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

pipette morphology. The pre-installedSutter Pipette Cookbook, glossary and helptopics, make it easy to access the extensivelibrary of programs. Both pullers offer:microprocessor controlled programmability,constant current power supply, a selfcontainedprecision air delivery system,and a patented velocity sensing system.These design elements have lead tosignificant improvements in pipettereproducibility when compared to othermicropipette pullers.The current state-of-the-art in micropipettepuller technology is the P-2000. Itincorporates the mechanical design andprogrammability of the Flaming/Brownpullers, but uses a CO 2laser as the heatsource. With the addition of the laser, quartztubing can now be pulled in addition to otherlower melting point glass compositions.Quartz pipettes have helped eliminate sometechnical barriers in electrophysiologicalstudies and have enhanced microinjectionprocedures as well as near field scanningmicroscopy (NSOM) and nanospray massspectroscopy.The P-30 is our lowest priced and leastsophisticated puller, based upon an NIHdesign from the 1950s. As a vertical puller,it is intended for basic requisites for apipette pulling device and provides a fewadditional features. It is suitable for basicmicropipette fabrication, sharp electrode,and microinjection work.The BV-10 micropipette beveler was thecompany’s first product and to date remainsthe state-of-the-art in micropipette bevelingtechnology. For precision beveling of pipettetip diameters from fractions of a micron totens of microns, it is the system of choice.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM5

P-2000 LASER BASEDMICROPIPETTE PULLERMICROPIPETTE FABRICATIONFEATURES P-2000 Capable of pulling quartz, borosilicate andaluminosilicate glass Fully programmable — including heatingfil a m e n t c h a r a c t e r i s t i c s The laser has no melting point limit as withconventional metal filaments, and therefore,cannot be burned out Pulls electrodes with tip diameters that areless than 0.03µm Optimized velocity sensing circuit formaximized sensitivity and reproducibility* Operating life of the CO 2laser is expectedto be in excess of ten years with normaluse, after which the laser can be refurbishedby Sutter Instrument for a nominal charge Individual programs can be write-protectedin order to secure them from inadvertentchanges The total time that the heat is on during thepull is displayed for improved programdevelopment and troubleshooting A date and time stamp is displayed to showwhen the program was written and/or thelast time a program was changed The P-2000/F is ideal for applications suchas nanospray and NSOM Preprogrammed sample programs forintra-cellular and patch pipettes. Specialprogramming upon request*Patent No.4,600,4246 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The P-2000 integrates a CO 2laser-based heat source with the technology derived from our extensiveexperience with conventional pullers. This system offers capabilities unmatched by other pullers.A significant advance in the technology of fabrication of micropipettes, optical fiber probes, andnanospray tips, is offered with the P-2000 micropipette puller.The use of laser heat allows the P-2000 to work with quartz glass (fused silica) as well asconventional glasses. Quartz offers superior material properties for a variety of research applications.Quartz is stronger than other glasses and can facilitate penetration through tough tissues whichwould normally break conventional pipettes 1 . For applications requiring a low noise glass, users willfind that quartz is the lowest noise glass available 2,4 . Quartz contains none of the metals used inconventional glasses 3 . Optically, quartz is virtually free from fluorescence when illuminated.MICROPIPETTE FABRICATIONA CO 2laser was selected as the heat source for the P-2000 for several reasons: 1) The nominalemission wavelength of the laser approximates the resonant frequency of the SiO 2lattice in glass.Thus, quartz and other conventional glasses can be melted when the appropriate laser power issupplied. 2) Laser heat is clean and leaves no metal residue on the pipette as do conventional heatingfilaments. 3) Laser heat can be turned off instantly, leaving no residual filament heat. 4) The user canprogram the amount and distribution of heat supplied to the glass. 5) No filaments to age or burn out.The P-2000 can store up to 100 separate programs, with each program consisting of up to 8 commandlines. Programmable parameters include: laser power level, scan width, trip velocity, delay/laser ontime, and hard pull strength.One important consideration for the use of the P-2000 is the diameter of the glass used. The opticaldesign produces even heating on glass up to 1.2 mm in outside diameter. Larger diameter glassescan be used with the P-2000/G (up to 1.5 mm quartz and 1.8 mm conventional glasses), but theperformance is best with glass that is 1.2 mm diameter or less.The P-2000/F works well with small diameter glasses such as optical fibers, and with small diameterfused silica capillary commonly used for the manufacture of nanospray tips. Smaller diameter glasswith an outer diameter in the range of 0.125 mm to 0.6 mm, require special puller bars as well as anoptical alignment optimized for the smaller diameter material. These modified components will beinstalled at the time of purchase.As with larger diameter glass, a wide range of tip sizes and taper geometries can be produced withthis modified P-2000/F and small diameter glass. We have drawn optical fiber tips ranging from lessthan 10nm to more than 5µm. Please consult our technical staff for further information.1, 2, 3, 4References listed on page 9PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM7

SPECIFICATIONS P-2000MICROPIPETTE FABRICATION Dimensions Weight Electrical30in x 14.25in x 13.25in76cm x 36cm x 33.5cm80lbs36kg115/230 Volts50/60 Hertz power line CLASS I LASER PRODUCTUS PRICES P-2000International prices are 5% higher. Prices subject to change without notice. P-2000/G Laser-based puller, outfi tted for use with glass $ 14,595GREATER than 0.6mm outer diameter P-2000/F Laser-based puller, outfi tted for use with glass $ 15,595LESS than 0.6mm outer diameter(Pullers include a sample box of Q100-70-7.5 glass)ACCESSORIES P-2000 FPS Spacer for special procedures $ 25 GS 1 Glass stop $ 75 GLA 1 Glass loading aid $ 150 BX10 2 Pipette storage box (holds 10) $ 154 3/4 x 3 5/8 x 3/4 inches BX20 2 Pipette storage box (holds 20) $ 157 x 3 5/8 x 3/4 inches1Installs on either puller bar2Minimum order of any 2 boxes8 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

REFERENCES P-20001. Munoz, J.L. and Coles, J. Quartz micropipettes for intracellular voltage microelectrodes and ionselective microelectrodes. Journal of Neuroscience Methods: 22:57-64, 1987.2. Rae, J.L. and Levis, R. A. A method for exceptionally low noise single channel recordings.European Journal of Physiology - Pflügers Archiv: 420:618-620, 1992.3. Zuazaga, C. and Steinacker, A. Patch-clamp recording of ion channels: Interfering effects of patchpipette glass. News in Physiological Science: 5:155- 159, 1990.4. Levis, R. A. and Rae, J. L. The use of quartz patch pipettes for low noise single channel recording.Biophysical Journal: 65:1666-1677, 1993.MICROPIPETTE FABRICATIONGlass StopGlass Loading AidPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM9

P-1000 NEXT GENERATIONMICROPIPETTE PULLERMICROPIPETTE FABRICATIONFEATURES P-1000 Color touch screen display Safe heat mode to protect and extendfil a m e n t li f e Pipette Cookbook program directory Line repeat mode simplifies multi-lineprogramming Help topics and error detection Glossary defines micropipette and pullerterminology Copy & paste function for writing newprograms Jaw temperature sensor helps define idealpulling conditions Record of last two pull results Ramp test more easily accessed and cannow be stored and referenced within eachprogram. Helps to establish program heatsettings and protect filament. Two symmetrical pipettes with each pull Self-contained air supply with filtrationsystem and humidity control chamber Memory storage for up to 100 programs Two cooling modes: time and delay10 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Developed through years of experience with the Flaming-Brown style micropipette pullers,and infused with leading-edge technology, Sutter is proud to introduce the P-1000 micropipettepuller. The most obvious new feature is the color touch-screen display that provides an intuitiveand full featured interface.The extensive library of programs found in the popular Sutter Pipette Cookbook has beenincorporated into the P-1000 puller and are available to the user. You need only specify the glass,filament, and type of pipette you require, and a suitable program is identified and available forinstallation. This takes the guesswork out of pipette pulling and simplifies programming.Copy and Paste functions assure that programs can be easily written and the line repeat modesimplifies multi-line programming. A safe heat mode is an additional feature that helps the useravoid using heat settings that might damage or burn out the filament. When the safe heat modeis turned on, the puller will “check” the installed heat and alert the user when a given heat valueis too low or too high in relation to the ramp value. User notes can be added to each program forannotating important information.MICROPIPETTE FABRICATIONNew features for the P-1000 include: diagnostic testing of all puller components, built-in errordetection of air pressure loss or filament burnout, easy access to ramp test, measurement ofjaw temperature, and access to previous pull results with the heat-on times for each cycle of theprogram. Help topics are preloaded to assist with on-site troubleshooting, and the built-in glossaryincludes text, pictures, and diagrams explaining the terms used in micropipette fabrication. Arotary dial is offered as an alternative to the touch pad for numerical entry.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM11

SPECIFICATIONS P-1000MICROPIPETTE FABRICATION Dimensions Weight Electrical21in x 14in x 12in53cm x 36cm x 30cm41lbs18.59kg115/230 Volts50/60 Hertz power lineRoHS CompliantUS PRICES P-1000International prices are 5% higher. Prices subject to change without notice. P-1000 Flaming/Brown type micropipette puller, manual, $ 7,950hard copy of Sutter Pipette Cookbook.Each puller comes with an assortment of 4 filaments and a sample boxof BF100-50-10, BF150-110-10, and BF150-86-10 glass. Sutter pre-programsthe P-1000 with a 2.5 x 2.5mm box filament (FB255B) unless an alternativefilament is requested.12 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

ACCESSORIES P-1000 FPS Fire polishing spacer for P-1000 pullers $ 25 GS 1 Glass stop $ 75 CTS Ceramic tile for scoring glass $ 15 BX10 2 Pipette storage box (holds 10) $ 154 3/4 x 3 5/8 x 3/4 inches BX20 2 Pipette storage box (holds 20) $ 157 x 3 5/8 x 3/4 inches1Installs on either puller bar2Minimum order of any 2 boxesMICROPIPETTE FABRICATIONGlass StopPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM13

P-97 FLAMING/BROWNMICROPIPETTE PULLERMICROPIPETTE FABRICATIONFEATURES P-97 Environmental chamber for humidity control Programmable air pressure Memory storage for up to 100 programs Write protection and date stamp for eachprogramf Two symmetrical pipettes with each pull Two cooling modes: time and delay Pre-programmed sample programs forintracellular and patch pipettes. Specialprogramming on request Ramp test to establish program heat settingswhen a new filament or glass is introduced Vacuum fluorescent display Constant current power supply for filamentand pull solenoid Looping pull cycle for fabrication of patchtype micropipettes Self-contained air supply with filtrationsystem and humidity control Consistent and reliable electrodes with tipdiameters less than 0.1µm Control over the time and pressure at whichthe air is delivered Optimized velocity sensing circuit formaximized sensitivity and reproducibility Quality control, SEM photograph of atip pulled with each puller; criterion is tipmeasurement less than 0.1µm and typicallyis ~0.06µm14 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The P-97 Flaming/Brown type micropipette puller is ideal for fabricating micropipettes, patchpipettes and microinjection needles. While retaining many of the features of earlier models,the P-97 offers improvements in mechanical, electronic and software design. The result isbetter control of the pulling process and a higher degree of reproducibility. The P-97 combinesa proven mechanical system with a sophisticated, programmable microprocessor controller.This programmable control of the pulling parameters allows the investigator to designapplication specifi c pipettes from a wide range of glass compositions and sizes.A number of other features have been incorporated in the design of the P-97. Most apparent isthe environmental chamber which surrounds the heating filament. This environmental chamberis designed to minimize the effect of changing humidity on the reproducibility of pulled pipettes.A 25% increase in power over the P-87 allows for the use of larger heating filaments, largerdiameter glass and multi-barreled glass. The metal jaws that clamp the heating filament havealso been redesigned to minimize heat retention. There are two modes of cooling: time anddelay. The delay mode provides extended cooling for large diameter and multi-barreled glass.A spring-loaded clamping mechanism has been added for easier loading of glass. A vacuumfluorescent display has been added that allows easy viewing.MICROPIPETTE FABRICATIONSoftware improvements on the P-97 include a display of the total heat-on time on to assist inprogram development and troubleshooting. Up to 100 programs can now be written and storedin memory, which makes the P-97 suitable for multiple users. These programs can now bewrite-protected, adding security to prevent programs from being changed or alteredinadvertently. The display shows the last date and time the program was written or edited.In addition, the air pressure is a programmable parameter.* Patent No. 4,600,424PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM15

SPECIFICATIONS P-97MICROPIPETTE FABRICATION Dimensions Weight Electrical21in x 16in x 12in53cm x 40.6cm x 30cm50lbs23kg115/230 Volts50/60 Hertz power lineREFERENCES P-97These references describe the Flaming/Brown series of pullers and contain valuable informationapplicable to the P-97.1. Brown, K.T. and Flaming, D.G. Neurosciences Journal: 2:813-827, 1977.2. Flaming, D.G. and Brown, K.T., Journal of Neuroscience Methods: 6:91-102, 1982.3. Brown, K.T. and Flaming, D.G., Advanced Micropipette Techniques for Cell Physiology.John Wiley and Sons. Great Britain, 1986.16 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES P-97International prices are 5% higher. Prices subject to change without notice. P-97 Flaming/Brown type micropipette puller, manual, $ 7,090hard copy of Sutter Pipette Cookbook.Each puller comes with an assortment of 4 filaments and a sample box ofBF150-110-10, BF100-50-10, and BF150-86-10 glass. Sutter pre-programs theP-97 with a 2.5mm box filament unless an alternative filament is requested.ACCESSORIES P-97 FPS Fire polishing spacer for P-97 and P-98 pullers $ 25 CTS Ceramic tile for scoring glass (large tips 20-200 microns) $ 15 BX10 1 Pipette storage box (holds 10) $ 154 3/4 x 3 5/8 x 3/4 inches BX20 1 Pipette storage box (holds 20) $ 157 x 3 5/8 x 3/4 inchesMICROPIPETTE FABRICATION1Minimum order of any 2 boxesPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM17

P-30 VERTICALMICROPIPETTE PULLERMICROPIPETTE FABRICATIONFEATURES P-30 Pulls electrodes with tip diameters down to0.3µm, consistently and reliably A micrometer allows precise reproducibilityof trip point settings in producing finemicroelectrodes Full three digit digital controls for accuratesetting of heat and pull values Constant current power supplies forfilament and pull solenoid Enclosed front to reduce variability causedby drafts Dual (manually) switched heat settingsfor patch pulling or two different types ofmicropipettes All working parts are made from corrosionresistant material Two heating assemblies available:platinum/iridium (recommended) orNichrome coil Built in RFI filter and dual voltage/dualfrequency operation Rubber padded jaws to minimize breakageof capillary tubing Designed to take up minimal bench space Slope of the front panel aids in preventingglass from entering cabinet/solenoidmechanism18 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The model P-30 vertical micropipette puller is designed for the fabrication of basic micropipettesand patch-type pipettes. It will pull micropipettes with tip diameters as small as 0.3µm andmoderate taper lengths (6 to 10mm). By using an included patching attachment, the P-30 will pulla patch-type pipette. But, for those needing very short tapers, high cone angles, and advancedreproducibility, one should consider the P-97. Using thin or standard walled capillaries, the P-30will generate suitable pipettes for microinjection studies. The P-30 is also ideal for studentlaboratories and other situations which call for an economical, reliable pipette pulling device.The P-30 is available with either a platinum/iridium or a Nichrome filament. The Nichromefilament is suitable for many applications and is not prone to damage. The platinum filament ismore effi cient at heating and cooling, and, although it is more prone to damage than the Nichromefilament, the platinum filament is recommended for thick wall and aluminosilicate glass andapplications requiring shorter taper lengths.MICROPIPETTE FABRICATIONPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM19

SPECIFICATIONS P-30MICROPIPETTE FABRICATION Dimensions Weight Electrical18in x 10in x 9in46cm x 25cm x 23cm35lbs16kg115/230 Volts50/60 Hertz power lineUS PRICES P-30International prices are 5% higher. Prices subject to change without notice. P-30/P Vertical micropipette puller with platinum/iridium $ 3,519filament P-30/N Vertical micropipette puller with Nichrome $ 3,519filamentEach puller comes with an assortment of 3 filaments (for P-30/P), sample box ofBF100-50-10 glass, and manual.ACCESSORIES P-30 P-30-NFL/M 1 Nichrome fi lament block assembly $ 200 P-30-PFL/M 1 Platinum/iridium fi lament block assembly $ 200 BX10 2 Pipette storage box (holds 10) $ 154 3/4 x 3 5/8 x 3/4 inches BX20 2 Pipette storage box (holds 20) $ 157 x 3 5/8 x 3/4 inches1Only necessary when changing filament configuration2Minimum order of any 2 boxes20 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

MICROPIPETTE PULLER COMPARISON CHARTFeatures P-1000 P-97 P-2000 P-30Heat SourcePlatinum Filament with Safe Heat ModePlatinum FilamentCO 2LaserPlatinum or Nichrome FilamentGlass TypeBorosilicate and Aluminosilicate Quartz Glass or Fiber 1Max. Glass Size (OD)4mm 2mm1.65mm Quartz, 1.8mm BorosilicateTip Size0.06µ - 3µ 0.03µ - 5µ 0.3µ - 2µ Max. Taper Length1cm1.8cm2cm Program Lines8 1 Stage or 2 Stage with manual adjustment 4 plus Line Repeat Mode Type of CoolingCompressed Dry Air with Humidity Control Chamber NA (Laser On/Off)NoneUsable Pipettes/Pull 2 2 2 1Price 2 $7,950 $7,090 $14,595 (Glass) $3,519$15,595 (Fiber)MICROPIPETTE FABRICATION1. Applicable only for the P2000/F2. U.S. prices. International prices are 5% higherPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM21

FILAMENTS/ACCESSORIESMICROPIPETTE FABRICATIONThere is a minimum purchase of 4 filaments.Appropriate filament selection depends on your research application, but a general guideline forfilaments is as follows:Box Filaments are recommended for large diameter, double barreled, or aluminosilicate glass.Box filaments are particularly suitable for patch pipettes, slice preparations, and microinjection,where long, parallel walls would aid penetration. When using a box filament, the size of the squarebox should be approximately 1.0mm to 1.5mm larger than the outside diameter of the glass thatyou will be using.For IVF and ICSI applications, a 2.5mm x 4.5mm box filament (FB245B) is recommended. Forpronuclear injection work, we recommend a 2.5mm x 2.5mm box filament (FB255B). Call for morespecifi c advice or to request a combination of settings, filaments, and glass for your application.Trough Filaments are excellent general purpose filaments and are recommended for patchpipettes produced from thin wall glass. If very short tapers are required for patch or microinjection,a 2.5mm or 3mm box filament is recommended.Sutter pre-programs the P-1000 and P-97 with a 2.5mm x 2.5mm box filament unless an alternative filament isrequested.For either shape of filament (box or trough), increasing the filament width tends to increase thelength of the pipette taper. If there are specifi c questions as to the filament type needed, pleasecall and our technical support staff will guide you in your choice.22 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

BOX FILAMENTSP-1000, P-97, P-87, P80PC, P80C, PC-84, P-77BFB215B 2.0mm square box filament, 1.5mm wide $ 35FB220B 2.0mm square box filament, 2.0mm wide $ 35FB230B 2.0mm square box filament, 3.0mm wide $ 35FB255B 2.5mm square box filament, 2.5mm wide $ 35FB245B 2.5mm square box filament, 4.5mm wide $ 35FB315B 3.0mm square box filament, 1.5mm wide $ 35FB320B 3.0mm square box filament, 2.0mm wide $ 35FB330B 3.0mm square box filament, 3.0mm wide $ 35TROUGH FILAMENTSP-1000, P-97, P-87, P80PC, P80C, PC-84, P-77BFT315B 1.5mm wide trough filament $ 35FT320B 2.0mm wide trough filament $ 35FT330B 3.0mm wide trough filament $ 35FT345B 4.5mm wide trough filament $ 35MICROPIPETTE FABRICATIONP-30 FILAMENTSP30T15 1.5mm wide trough filament $ 35P30T20 2.0mm wide trough filament $ 35P30T30 3.0mm wide trough filament $ 35P30N Nichrome filament $ 35P-77A LOOP FILAMENTS(Serial number 160 and above)FL315A 3.0mm loop filament, 1.5mm wide $ 35FL320A 3.0mm loop filament, 2.0mm wide $ 35FL325A 3.0mm loop filament, 2.5mm wide $ 35(Serial number below 160)FL315X 3.0mm loop filament, 1.5mm wide $ 35FL320X 3.0mm loop filament, 2.0mm wide $ 35FL325X 3.0mm loop filament, 2.5mm wide $ 35ACCESSORIESFILAMENT Custom platinum/iridium filament $ 55FPS Fire polishing spacer for P-2000, P-97, $ 25and P-87 pullersFS1875 Platinum/iridium sheet, $ 35018mm x 75mm x 0.05mm (40µ)CTS Ceramic tile for scoring glass $ 15(large tips 20-200 microns)BX10 1 Pipette storage box (holds 10) $ 154 3/4 x 3 5/8 x 3/4 inchesBX20 1 Pipette storage box (holds 20) $ 157 x 3 5/8 x 3/4 inches1Minimum order of any 2 boxesPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM23

BV-10MICROELECTRODE BEVELERMICROPIPETTE FABRICATION(BV-10-D with optional 80X stereo microscope)FEATURESBV-10 Vibration-free, magnetically coupledbeveling surface Abrasive surface optically fl at to a halfwave (250nm) Finest abrasive surface commerciallyavailable 7 pound steel baseplate adds additionaldampening Integrated halogen lamp Robust micromanipulator controls bevelangle and advancement Synchronous clock motor insures stablerotation ratef24 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Elegant and simple to use, the BV-10 offers precision beveling of micropipette tips between0.1 and 100µm. The unique abrasive plate drive system is vibration free for greater control of thebeveling process. Beveling can be accomplished very rapidly and produces consistent tip diametersusing the techniques as described by Brown and Flaming, Science, August 1974, Vol. 185.Intracellular recording electrodes can benefi t from beveling because of 1) a reduction in the tipdiameter by creation of the sharp point on the electrode and 2) a lowered electrical resistanceof the electrode due to the larger cross sectional area of the lumen. This greatly facilitatespenetrating and holding very small or diffi cult cells. Microinjection needles also benefi t frombeveling by promoting entry into cells with minimal damage while at the same time enhancing thefl ow of material through the needle.MICROPIPETTE FABRICATIONThe basic beveling system consists of a stationary pedestal, optically fl at to a half wave (250nm),surface mounted on a heavy baseplate. This serves as a bearing for an abrasive coated glassgrinding plate, which is also fl at to half a wave. The fl at abrasive plate is coupled to a lowvibration, slow-speed motor by means of magnetic fields to provide a wobble-free fl at grindingsurface. The abrasive plates are fabricated with a proprietary process which insures aconsistent abrasive coating.A 2-axis micromanipulator holds the pipette to be beveled and permits controlled advancementonto the abrasive surface. The bevel angle and speed of advancement are adjustable. A halogenlamp with a gooseneck enhances the beveling operation by providing sharp illumination of theabrasive plate and pipette.The basic system comes with two abrasive plates of your choice, a wick with holder (for wetbeveling), pedestal oil, degreasing fluid, and manual.Two options are available for monitoring the beveling process: an 80X stereo microscope, and anelectrode impedance meter. Depending on your research application, one or both of these optionsmay be desirable. For all micropipette applications, the swing mounted microscope enhances yourcontrol of pipette advancement onto the abrasive plate and allows for viewing of the bevelingoperation (scope resolution is not suffi cient for viewing the actual bevel except in the case ofvery large tips). For microelectrode applications, the impedance meter is used to monitor the tipresistance during the beveling operation. The meter is an analog design, offering three resistanceranges (0-10, 0-100, 0-500 MOhm). Measurements are made at 12 Hz to minimize capacitivecontributions to the impedance measured and provide a near-true DC resistance value. A rapidroll-off is used to reduce 50/60 Hz interference, allowing operation in a laboratory environmentwithout screening.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM25

SPECIFICATIONSBV-10MICROPIPETTE FABRICATION Bevel Range Grinding SurfaceVariation Grinding Speed Bevel Angle Range Micromanipulator Dimensions Speed Weight Electrical0.1µm through 100µm finished electrodesdepending on abrasive plate usedLess than 1.0µm60 RPM5-90 degrees - adjustableCourse drive: 0.075in / dial revolutionFine drive: 0.0004in / dial revolution19in x 9in x 8in48cm x 22cm x 20cmApprox. 45lbs/20kg120 volts - 50/60 Hz power line(220 volt option requires specialmodifi cation, please see price list)RoHS Certified(with 220V-MOD)OPTIONS80X stereo microscopeImpedance meter for real-time measurement of tip impedence26 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES BV-10International prices are 5% higher. Prices subject to change without notice. BV-10-B 1 Micropipette beveler basic system $ 3,090 BV-10-C BV-10-B with electrode impedance meter $ 3,995 BV-10-D BV-10-B with 80X stereo microscope $ 3,995 BV-10-E BV-10-B with impedance meter and 80X stereo $ 4,950microscope1Includes micromanipulator, reference wick, reference wick holder, pedestal oil,degreaser, manual, and two abrasive plates of your choice.ACCESSORIESBV-10MICROPIPETTE FABRICATION BV-10M Electrode impedance meter with active $ 1,415and reference lead BV-10S 80X stereo microscope $ 950 104C Diamond abrasive plate – coarse $ 160( 5.0µm to 50µm tip sizes ) 104D Diamond abrasive plate – fine $ 160( 2.0µm to 20µm tip sizes ) 104E Diamond abrasive plate – very fine $ 160( 0.7µm to 2.0µm tip sizes) 104F Diamond abrasive plate – extra fine $ 160( 0.2µm to 1.0µm tip sizes) 007 Degreaser (bottle) $ 12 008 Beveler pedestal oil $ 12 220V-MOD Modifi cation to 220 volts $ 220REPLACEMENT PARTSBV-10 101 6-inch reference lead (body to meter) $ 12 102 2-inch active lead (platinum to pipette) $ 12 X050300 Reference wick $ 7 M100019 Reference wick holder $ 32 O664111 Halogen bulb $ 14 H906100 Drive belt $ 20PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM27

MANUAL MICROINJECTORMICROPIPETTE FABRICATIONThe Manual Injector is a manual syringe driver for pneumatic or hydraulic control of injectionneedles or holding pipettes. It is suitable for injecting volumes in the nanoliter to microliter range.It is also widely used as a fluid control device for applications requiring sensitive manual controlof the displacement of microneedle contents. The system is constructed from the highest qualityparts. The base assembly is black anodized aluminum. A non-rotating Mitutoyo micrometerprovides the drive to the gas-tight syringe 1 . A precision 3-way valve provides a convenient methodfor filling the fluid line and clearing air bubbles from the line. Tefl on tubing, chromatographyconnectors and a pipette holder 2 complete the system. The injection resolution is dependent onthe volume of the syringe that is installed, as outlined in the table below. For example, with a 25µlsyringe installed, one complete rotation of the micrometer (25 divisions) yields a displacementequivalent to a volume of 267nl and turning the micrometer one division (0.001in) yields 10.6nl.SYRINGE VOLUME VOLUME PER REVOLUTION VOLUME PER DIVISION10µl 106nl 4.2nl25µl 267nl 10.6nl50µl 529nl 21.2nl100µl 1.06µl 42.3nl250µl 2.65µl 105.8nl500µl 5.29µl 211.7nl1000µl 10.58µl 423.4nl1Please specify the syringe volume that you will be using. For animal IVF applications, 500µl and 1000µlsyringes are commonly used.2Unless specified otherwise, a pipette holder for 1mm outside diameter glass will be supplied. The diameterof the pipette holder is 0.25 inches (6.4mm). For micromanipulators that cannot accept this size holder, anadapter is optionally available. Please contact us for further information.28 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

SPECIFICATIONSMANUAL MICROINJECTOR Dimensions Weight12in x 3.75in x 4in30cm x 9.5cm x 10cm2.5lbs1kgUS PRICESMANUAL MICROINJECTORInternational prices are 5% higher. Prices subject to change without notice. MANUAL Manual injector with non-rotating micrometer, 3-way $ 1,250valve, precision 50µl gas-tight syringe, tefl on tubing,connectors and MI-10010 pipette holder assembly for1.0mm capillary tubing *MICROPIPETTE FABRICATION* Other syringe volumes, and pipette holder sizes are available upon request.ACCESSORIESMANUAL MICROINJECTOR MI-10010 Pipette holder assembly (specify 1.0mm, 1.2mm, $ 58or 1.5mm OD glass) includes holder and mounting rod V200050 Tefl on tubing $ 4 per ft. V001180 10µl gas-tight syringe $ 110 V001181 25µl gas-tight syringe $ 110 V001182 50µl gas-tight syringe $ 110 V001183 100µl gas-tight syringe $ 110 V001184 250µl gas-tight syringe $ 110 V001185 500µl gas-tight syringe $ 110 V001186 1000µl gas-tight syringe $ 110PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM29

MICROPIPETTE FABRICATIONMICROPIPETTE TECHNIQUESADVANCED MICROPIPETTE TECHNIQUESFOR CELL PHYSIOLOGYKENNETH T. BROWNUniversity of California atSan FranciscoDALE FLAMINGSutter Instrument Company,Novato, CA.Fine glass micropipettes are extensively used in intra- and extracellular physiology as a meansof recording electrical activity in cells and as channels for injecting a variety of substances forexperimental purposes. In 1973, the authors began a course of systematic studies designed tohelp them improve the capabilities and effi ciency of intracellular research using the micropipettetechnique. Here, they present for the first time their theory of how micropipette tips are formed,their methods of reducing tip size, and the implications of their work for research on small cells ofall kinds, especially cells within the central nervous system. This text not only incorporates thisnew work, but reviews and analyzes existing publications on micropipette methodology, includingpatch-clamping, in order to present as complete an account as possible of how micropipettes canbe used effi ciently and effectively in a wide variety of experimental situations. The informationpresented here should prove helpful to anyone performing research with micropipettes, from agraduate student conducting a first project to the most experienced investigator.30 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

CONTENTSEarly Methods of Fabricating MicropipettesThe Flaming/Brown Micropipette Puller:Its Background, Design and Underlying PrinciplesTechniques for Examining and MeasuringMicropipette Tips by Scanning Electron MicroscopyEvaluation of Flaming/Brown Micropipette PullerA Theory of Micropipette Tip Formation: QuantitativePrediction and Validation of the Effects of CapillaryWall Thickness Upon Tip SizeEffects of a Fused Internal Fiber (Omega Dot) UponMicropipette TipsMinimizing Tip Size With Borosilicate TubingAncillary Techniques for Conducting IntracellularResearchEvaluation of Improved Intracellular RecordingTechniques in Vertebrate PhotoreceptorsEvaluation of Tubing Designs for Intracellular WorkThe Structure Properties of Glasses for FabricatingMicropipettesDual-Channel MicropipettesThe Burgeoning Field of Patch ClampingExtension of the Flaming/Brown Micropipette Pullerto Patch Clamping and Conveniently HandlingAluminosilicate GlassMICROPIPETTE FABRICATIONBeveling Micropipette Tips: Techniques andApplicationsFilling Micropipettes: Techniques and SolutionsAdvancing Micropipettes Through Tissues and IntoCellsReferencesAppendicesUS PRICES BOOKInternational prices are 5% higher. Prices subject to change without notice. BOOK Advanced Micropipette Techniques $ 30For Cell PhysiologyPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM31

GLASS CAPILLARY TUBINGMICROPIPETTE FABRICATIONSutter Instrument, in addition to the finest micropipette pullers available, offers a wide selectionof high quality glasses in various sizes and materials. Though there are many types and sizesof glass available, we have carefully selected only those that pass our criteria. Our expertise inmicropipette technology assures you of precision and high quality.We offer capillary tubing in three different compositions: quartz, borosilicate and aluminosilicate.Each composition has its own unique properties and the selection will be determined by yourapplication and your puller’s capabilities.Filamented glass has a small rod of glass annealed to the inner wall and this rod (filament of glass)creates the capillary action required to back-fill the pipette with solution. If the resulting pipette tipis under 1ul and being used for microinjection or recording, we recommend “filamented-glass.” Thefilament in the glass not only provides capillary action for quick filling of the micropipette, it alsohelps to reduce the incidence of air bubbles when introducing solution into the pipette. If you haveany additional concerns, please contact Sutter for technical support.32 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

FIRE POLISHINGAll borosilicate and aluminosilicate capillary glass offered by Sutter Instrument have fire-polished ends.This process eliminates any sharp edges, making it easier to insert into holders, and does not affectthe electrical or mechanical properties of the glass.CUSTOM PIPETTESSutter Instrument can make custom pipettes and microtools not commonly available from other pipettemanufacturers. The custom pipettes are considered non-sterile and are manufactured for researchapplications and non-human use. Please contact Sutter Instrument for further details.BOROSILICATE (CORNING 7740)The most commonly used glass is borosilicate. Sutter Instrument offers only TYPE I-CLASS A borosilicateas described by ASTM Standard 3.1.2 1 . This glass softens at 821 degrees Celsius and, as it is pulled,maintains its ratio of inside diameter to outside diameter over the total taper length. Borosilicatesoftens at a lower temperature than our other glasses and has a wider working range. Theseunique properties allow for a greater variety of shapes used in microelectrodes, patch pipettes,microinjection needles and, in the case of solid rod, chromosome dissection tools.ALUMINOSILICATE (SCHOTT 8252)Aluminosilicate softens at a higher temperature (935 degrees Celsius) than borosilicate and is workableover a much narrower range. It has a tendency to continuously thin out as it is drawn which allowsextremely fine tips with very short tapers. For example, we have pulled aluminosilicate tips in the 20-30nanometer range with taper lengths of 5-6mm. Its resistivity is several orders of magnitude higher thanborosilicate, thus reducing leakage currents when used in ion-selective micropipettes. Aluminosilicate isharder than borosilicate which results in a pipette that is more suitable for penetrating tough tissues.QUARTZ (HERAEUS HSQ300)The finest and purest glass available is quartz. It is superior to all other glasses in its mechanical, electricaland optical qualities. It has the lowest dielectric constant, the lowest loss factor and the highest volumeresistivity making it ideal for patch clamp recording. Its chemical purity virtually eliminates leakage ofions 2 and by using quartz in single channel patch clamp recordings the lowest background noise levelshave been achieved 3 . Due to its high melting point, it cannot be pulled on conventional pullers, but canbe easily pulled with the Sutter CO 2laser-based P-2000.SIZESSutter Instrument capillary tubing is available in a broad range of wall thicknesses which allow you toselect the size necessary for your application. The ratios of inside diameter to outside diameter typicallyrun between 0.5mm and 0.75mm with the lower value being referred to as thick wall tubing and thetop of the range as thin wall tubing. All other factors being equal, the thicker wall capillaries producepipettes with longer tapers and smaller tips which make it more suitable for intracellular microelectrodes.These thicker wall pipettes tend to reduce the noise contributions due to capacitance, which makesthem more suitable for patch clamp pipettes. Thinner wall tubing allows for larger pore openings whichmakes it ideal for microinjection needles and low resistance microelectrodes.MICROPIPETTE FABRICATION1ASTM Designation E438-90 - April 1990.2Zuazaga C., Steinacker A. Patch-clamp recording of ion channels: Interfering effects of patch pipette glass.News in Physiological Sciences: International Union of Physiological Sciences and the American PhysiologicalSociety: 5:155-158, August 1990.3Rae, James L., Levis Richard A. A Method for exceptionally low noise single channel recordings. Pflügers Archive;European Journal of Physiology:420:618-620, Springer-Verlag 1992.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM33

MICROPIPETTE FABRICATIONSTANDARD WALL BOROSILICATE TUBINGWITH FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceBF100-50-7.5 1.00mm 0.50mm 7.5cm 225 $ 43BF100-50-10 1.00mm 0.50mm 10cm 225 $ 40BF100-50-15 1.00mm 0.50mm 15cm 225 $ 54BF100-58-10 1.00mm 0.58mm 10cm 250 $ 39BF100-58-15 1.00mm 0.58mm 15cm 250 $ 50BF120-60-10 1.20mm 0.60mm 10cm 225 $ 46BF120-69-10 1.20mm 0.69mm 10cm 250 $ 41BF120-69-15 1.20mm 0.69mm 15cm 250 $ 36BF150-75-10 1.50mm 0.75mm 10cm 225 $ 46BF150-86-7.5 1.50mm 0.86mm 7.5cm 250 $ 43BF150-86-10 1.50mm 0.86mm 10cm 250 $ 43BF150-86-15 1.50mm 0.86mm 15cm 250 $ 82BF200-100-10 2.00mm 1.00mm 10cm 225 $ 86BF200-116-10 2.00mm 1.16mm 10cm 250 $ 94BF200-116-15 2.00mm 1.16mm 15cm 250 $ 99WITHOUT FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceB100-50-10 1.00mm 0.50mm 10cm 225 $ 40B100-50-15 1.00mm 0.50mm 15cm 225 $ 78B100-58-10 1.00mm 0.58mm 10cm 250 $ 36B100-58-15 1.00mm 0.58mm 15cm 250 $ 47B120-69-10 1.20mm 0.69mm 10cm 250 $ 45B120-69-15 1.20mm 0.69mm 15cm 250 $ 39B150-86-7.5 1.50mm 0.86mm 7.5cm 250 $ 43B150-86-10 1.50mm 0.86mm 10cm 250 $ 45B150-86-15 1.50mm 0.86mm 15cm 250 $ 75B200-116-10 2.00mm 1.16mm 10cm 250 $ 66B200-116-15 2.00mm 1.16mm 15cm 250 $ 77HEAVY POLISHED STANDARD AND THIN WALLED TUBING 1WITH FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceBF150-86-7.5HP 1.50mm 0.86mm 7.5cm 250 $ 50BF150-86-10HP 1.50mm 0.86mm 10cm 250 $ 50BF150-110-7.5HP 1.50mm 1.10mm 7.5cm 225 $ 50BF150-110-10HP 1.50mm 1.10mm 10cm 225 $ 48WITHOUT FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceB150-86-7.5HP 1.50mm 0.86mm 7.5cm 250 $ 50B150-86-10HP 1.50mm 0.86mm 10cm 250 $ 52B150-110-7.5HP 1.50mm 1.10mm 7.5cm 225 $ 50B150-110-10HP 1.50mm 1.10mm 10cm 225 $ 461Useful for added protection of gaskets and wire in headstages.34 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

THIN WALL BOROSILICATE TUBINGWITH FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceBF100-78-10 1.00mm 0.78mm 10cm 250 $ 36BF100-78-15 1.00mm 0.78mm 15cm 250 $ 52BF120-94-10 1.20mm 0.94mm 10cm 250 $ 42BF120-94-15 1.20mm 0.94mm 15cm 250 $ 56BF150-110-7.5 1.50mm 1.10mm 7.5cm 225 $ 43BF150-110-10 1.50mm 1.10mm 10cm 225 $ 42BF150-117-10 1.50mm 1.17mm 10cm 250 $ 38BF150-117-15 1.50mm 1.17mm 15cm 100 $ 42BF200-156-10 2.00mm 1.56mm 10cm 250 $ 46BF200-156-15 2.00mm 1.56mm 15cm 100 $ 50WITHOUT FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceB100-75-10 1.00mm 0.75mm 10cm 225 $ 36B100-75-15 1.00mm 0.75mm 15cm 225 $ 47B120-90-10 1.20mm 0.90mm 10cm 225 $ 39B120-90-15 1.20mm 0.90mm 15cm 225 $ 41B150-110-7.5 1.50mm 1.10mm 7.5cm 225 $ 43B150-110-10 1.50mm 1.10mm 10cm 225 $ 39B200-156-10 2.00mm 1.56mm 10cm 250 $ 45MICROPIPETTE FABRICATIONMULTIBARREL BOROSILICATE 1WITH FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package price2BF100-50-10 2 barrels 1.00/0.50 10cm 75 $ 512BF100-75-10 2 barrels 1.00/0.75 10cm 75 $ 492BF150-86-10 2 barrels 1.50/0.86 10cm 100 $ 562BF150-86-15 2 barrels 1.50/0.86 15cm 100 $ 723BF100-50-10 3 barrels 1.00/0.50 10cm 75 $ 503BF100-75-10 3 barrels 1.00/0.75 10cm 75 $ 493BF120-69-10 3 barrels 1.20/0.69 10cm 100 $ 533BF120-69-15 3 barrels 1.20/0.69 15cm 100 $ 72WITHOUT FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package price3B100-75-10 3 barrels 1.00/0.75 10cm 75 $ 491Multibarrel borosilicate requires a custom filament.Please contact Sutter Instrument for more information when ordering.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM35

MICROPIPETTE FABRICATIONQUARTZ TUBINGWITH FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceQF100-50-7.5 1.00mm 0.50mm 7.5cm 100 $ 113QF100-50-10 1.00mm 0.50mm 10cm 100 $ 162QF100-60-7.5 1.00mm 0.60mm 7.5cm 100 $ 104QF100-60-10 1.00mm 0.60mm 10cm 100 $ 157QF100-70-7.5 1.00mm 0.70mm 7.5cm 100 $ 130QF100-70-10 1.00mm 0.70mm 10cm 100 $ 157QF100-70-15 1.00mm 0.70mm 15cm 100 $ 172QF120-60-7.5 1.20mm 0.60mm 7.5cm 100 $ 162QF120-90-10 1.20mm 0.90mm 10cm 100 $ 117QF150-75-7.5 1.50mm 0.75mm 7.5cm 100 $ 117WITHOUT FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceQ100-30-7.5 1.00mm 0.30mm 7.5cm 100 $ 118Q100-30-15 1.00mm 0.30mm 15cm 100 $ 124Q100-50-7.5 1.00mm 0.50mm 7.5cm 100 $ 113Q100-50-10 1.00mm 0.50mm 10cm 100 $ 151Q100-70-7.5 1.00mm 0.70mm 7.5cm 100 $ 106Q100-70-10 1.00mm 0.70mm 10cm 100 $ 151Q120-40-7.5 1.20mm 0.40mm 7.5cm 100 $ 179Q120-60-7.5 1.20mm 0.60mm 7.5cm 100 $ 157Q120-90-7.5 1.20mm 0.90mm 7.5cm 100 $ 135Q120-90-10 1.20mm 0.90mm 10cm 100 $ 172Q150-50-7.5 1.50mm 0.50mm 7.5cm 100 $ 162Q150-75-7.5 1.50mm 0.75mm 7.5cm 100 $ 105Q150-75-10 1.50mm 0.75mm 10cm 100 $ 117Q150-110-10 1.50mm 1.10mm 10cm 100 $ 179Q165-115-10 1.65mm 1.15mm 10cm 100 $ 153SOLID QUARTZ RODcatalog outside inside overall piecesnumber diameter diameter length per package priceQR-100-7.5 solid 1.00 mm 7.5cm 100 $ 71QR-100-10 solid 1.00 mm 10cm 100 $ 71QR-100-15 solid 1.00 mm 15cm 100 $ 7136 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

QUARTZ THETA TUBINGcatalog outside inside overall piecesnumber diameter diameter length per package priceQT120-90-7.5 1.20/0.9mm 0.15mm 7.5cm 50 $ 76MULTIBARREL QUARTZcatalog outside inside overall piecesnumber diameter diameter length per package price7Q033-16-10 7 barrel 1.00/[.33/.16ea] 10cm 50 $ 229ALUMINOSILICATE TUBINGWITH FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceAF100-64-10 1.00mm 0.64mm 10cm 100 $ 130AF120-77-10 1.20mm 0.77mm 10cm 100 $ 130AF120-87-10 1.20mm 0.87mm 10cm 100 $ 130AF150-100-10 1.50mm 1.00mm 10cm 100 $ 130WITHOUT FILAMENTcatalog outside inside overall piecesnumber diameter diameter length per package priceA100-64-10 1.00mm 0.64mm 10cm 100 $ 110A120-77-10 1.20mm 0.77mm 10cm 100 $ 110A120-87-10 1.20mm 0.87mm 10cm 100 $ 110A150-100-10 1.50mm 1.00mm 10cm 100 $ 110MICROPIPETTE FABRICATIONBOROSILICATE THETA TUBINGcatalog outside inside overall piecesnumber diameter diameter length per package priceBT-150-10 1.50/1.17mm 0.165mm 10cm 100 $ 89SOLID BOROSILICATE RODcatalog outside inside overall piecesnumber diameter diameter length per package priceBR-100-10 solid 1.00mm 10cm 250 $ 43BR-100-15 solid 1.00mm 15cm 250 $ 45PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM37

MICROMANIPULATIONPrecise movement. Just what you require in amicromanipulator. Sutter Instrument, the samecompany that refined the movements necessaryto repeatedly pull micropipettes with sub-microntips, has also harnessed the technologiesnecessary to easily move those same tips withsub-micron accuracy.Micromanipulator design at Sutter Instrumentbegan in 1985 with the MP-85, a refined andextended Huxley-style micromanipulator.Continuing from that time Sutter Instrumenthas shown a desire to provide manipulatormechanisms with smooth, ergonometricmicro-movements that are also adaptableto many different experimental designs andplatforms. This design process includes fourmotorized manipulators, the industry standardMP-285, the MP-225, the narrow formatMP-265 and the Xenoworks BRM. All couplethe smooth mechanical micromotion andmicroprocessor-based, stepper-motor drivemechanisms users have come to expect inSutter products. In 2004, we introduced theMPC-200 controller, answering the demandof our customers who asked for a multimanipulatorcontroller.Researchers performing complex experimentsrequiring multiple manipulators are now ableto pair our popular motorized manipulatorswith the MPC-200 controller. This controlleris capable of operating any 2 of our motorizedmanipulators and can be expanded to run 4manipulators off a single ROE-200 input device.This solution is fl exible and allows the user toadd components as their needs dictate.The 3DMS, 3-Dimensional motorized stage,was designed for experiments that require38 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

apid movement of a chamber or culture dishto multiple locations in a compact space. Twoaxes provide X-Y translation and the thirdaxis provides built-in focusing with submicronaccuracy. The 3DMS offers roboticprogrammability of complex motion sequenceswhen used with the MP-285 controller.As time has shown, much of what makesa device popular is how easily it can beincorporated into an existing set-up. In thecase of micromanipulators, this process hasinvolved designing manipulator platforms thatbolt directly to the frames of the most popularmicroscopes. Alternatively, we also offerrock-steady, free-standing platforms thatsupport our manipulators by clamping to thetable beside the microscope. Our MT-70 andMT-75 stands, originally designed for stablesupport of the fi fteen pound Huxley, are perfectfree-standing platforms for the MP-225 andMP-285. The motorized manipulators are alsosupported by a number of microscope-specifi cstands that bolt directly to the bodies of themost popular Leica, Nikon, Olympus, andZeiss inverted microscopes. These platformslend themselves to the low-drift recordingconfi guration required when using the MP-225and MP-285 for positioning patch-electrodesonto attached cells in culture.A task specifi c platform for manipulators is theMT-1000 translation system. When coupledwith MP-285s or MP-225s, the system formsa slice-patch workstation. The MT-1000 wasborn out of a technique now common in slicerecording that moves the optical pathwaywhile keeping the slice chamber and recordingelectrodes fi xed. The translation system allowsthe user to move to multiple locations on thetissue at high resolution without disturbingrecording electrodes. The MT-1000 workstationencompasses: an X-Y translator designed tomove a microscope smoothly and accurately;two MT-75 gantry-type stands for positioningmanipulators on one or both sides of themicroscope; and a third gantry stand thatbecomes the fi xed support for the chamber.The newly introduced MT-2000 uses a motorizedX-Y translator. In this design, stepper-motordriven lead screws provide smooth movementof the microscope.Rounding out our line of manipulators are theMM-33 micromanipulator and the MM-1 andMM-3 micropositioners. The MM-33 is aneconomical micromanipulator of suffi cientresolution and control for impaling xenopusoocytes and other medium-fine manipulationtasks. The MM-1 and the MM-3 are suitablefor positioning perfusion tubes, moving apreparation, or for positioning referenceelectrodes in a bath.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM39

MPC-200/MPC-385/MPC-325MULTI-MICROMANIPULATOR SYSTEMSMICROMANIPULATION(Shown: MPC-385-2)FEATURESMPC-200 Single controller and ROE will run twostepper motor drive manipulators Self-detecting, daisy-chain capabilityallows control of four manipulators fromone ROE-200 User-friendly interface: single button accessto all major functions New Accelerated Mode for fast, manualmanipulator movement Easy toggle selection of Mode (speed/resolution, pulsed diagonal, AcceleratedMode) Display indicates X, Y, Z coordinates, Mode,active manipulator Robotic Home and Work Position moves foreasy automated pipette exchange Faster robotic moves than previous versions Definable 4th axis for coaxial pipettemovement, angle selected by DIP switches Simple USB interface Toggle switch selects which manipulator isconnected to input device LED and display indicate active manipulator Ultra-low drift, ultra-smooth movement(Patents pending)40 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Neurobiological experiments are becoming more complex. Many require multiple manipulatorswith control units that quickly become space and/or cost prohibitive. The MPC-200 is the solutionyou have been asking for. A single controller capable of running 2 manipulators! Sutter Instrumenthas taken the simplicity of the MP-225 controller and expanded it to run two manipulators froma single controller/ROE. The MPC-200 works with our world-renowned mechanicals, the MP-285(MPC-385), MP-225 (MPC-325) or narrow format MP-265 (MPC-365).If two manipulators aren’t enough, a second controller can be daisy chained to allow the singleROE-200 to move up to four manipulators. Thus the system can be easily expanded to controlhighly sophisticated experiments.MICROMANIPULATIONThe Sutter MPC-200 is electrically quiet. Unique to the MPC-200 is our multi-unit controller whichemploys linear output circuitry to minimize electrical noise. Sutter adds additional manipulatorsto the same controller without requiring potentially noisy chopper drives.Faster automated pipette exchange.The MPC-200 has faster “Home” and “Work Position” moves for quicker pipette exchange.Automation is set up and run via the same interface used in the MP-225 controller; however, thespeeds of the automated movement are much faster.Accelerated manual mode.For users who prefer manual pipette exchange we have added “Accelerated Mode” to the ROE.Using Accelerated Mode, the user can make quick manual moves in and out of a setup. AcceleratedMode amplifies the speed attainable in a manual move by smoothly accelerating to the maximumspeed during sustained, fast turns of the ROE. Accelerated movement ends as soon as the userstops turning the knob. This mode can be fully disabled for those who feel the need for directcontrol of the pipette.To provide the ultimate in fl exibility, the MPC-200 can be purchased separately or as basicsystems such as the MPC-385, MPC-365 and MPC-325. Additional component pieces can beadded at any time, allowing the user to develop a system tailored to his or her particular needs.The controller is self-detecting so there is no need for manual confi guration of the components.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM41

SPECIFICATIONSMPC-200MICROMANIPULATION Resolution and Minimal microstep size is 62.5Full Travelnanometers per microstep. Display hassingle micron resolution. Full travel is25mm in each axis. Maximum Speed MP225mechanical 3mm/sec.MP285mechanical 5mm/sec. DRIFT< 1 micron/hrDrive MechanismDIMENSIONS Controller16in x 11in x 3.5in41cm x 28cm x 9cm ROE10in x 6in x 4in25cm x 15cm x 23cmWEIGHT Controller6.5lbs3kg ROE3.5lbs1.6kg Electrical115/230 Volts50/60 Hertz power lineRoHS Compliant42 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES MPC-385 SYSTEMSInternational prices are 5% higher. Prices subject to change without notice. MPC-385 One MP-285 manipulator mechanical, one $ 8,500MPC-200 controller and one ROE-200. Alsoincludes mounting adapter plate, rod holder,hinged headstage mount, 4 inch dovetailextension, manipulator and ROE connectingcables, power cord and manual.* MPC-385-2 Same as the MPC-385, but with two MP-285 $ 13,900manipulator mechanicals.* MPC-385-3 Same as the MPC-385, but with three MP-285 $ 21,000manipulator mechanicals, and two MPC-200controllers.* MPC-385-4 Same as the MPC-385, but with four MP-285 $ 26,000manipulator mechanicals, and two MPC-200controllers.*MICROMANIPULATIONUS PRICES MPC-325 SYSTEMSInternational prices are 5% higher. Prices subject to change without notice. MPC-325 One MP-225 manipulator mechanical, one MPC-200 $ 7,450controller and one ROE-200. Also includes mountingadapter plate, rod holder, hinged headstage mount,4 inch dovetail extension, manipulator and ROEconnecting cables, power cord and manual.* MPC-325-2 Same as the MPC-325, but with two MP-225 $ 11,900manipulator mechanicals.* MPC-325-3 Same as the MPC-325, but with three MP-225 $ 18,000manipulator mechanicals, and two MPC-200controllers.* MPC-325-4 Same as the MPC-325, but with four MP-225 $ 22,000manipulator mechanicals, and two MPC-200controllers.* MPC-200-ROE Consists of the MPC-200 controller and ROE-200. $ 3,500This “system” is made available at a special pricefor those who wish to use previously purchasedSutter manipulators with the newer controllersystem or to use the MPC-200-ROE to controlother devices. Includes cable to connect ROEto controller, power cord and manual.* Indicate right- or left-handed set-up when orderingPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM43

COMPONENTSMPC-200MICROMANIPULATION MPC-200 Controller box alone. Purchase this if you intend to $ 2,800daisy-chain two controllers to one ROE-200. In thisconfi guration, up to 4 manipulators can be controlledby a single ROE-200.NOTE:This is not a stand-alone device. In order to function,the MPC-200 must be connected to a second MPC-200with ROE-200 attached or be connected to its ownROE-200. Includes daisy chain cable, power cordand manual. ROE-200 This is the ROE alone. Provides user input to one or $ 1,250more MPC-200 controllers. This component is intendedfor users who have an existing four-manipulatorMPC-200 system with a single ROE-200 and wish tosplit it into two independent two-manipulator systems.Includes cable to connect ROE to controller. MPC-285/M The MP-285 manipulator mechanical alone. This is $ 5,500the mechanical from the MP-285, the manipulatorsystem that made Sutter the world leader in motorizedmanipulators for electrophysiology. Over 2000 units,many of which have been in use over 5 years, allowus to stand behind the reliability of this design. It iswidely known for its smooth movement and low drift.Includes mounting adapter plate, rod holder, 4 inchdovetail extension, hinged headstage mount and cableto connect manipulator to controller.* MPC-225/M The MP-225 manipulator mechanical alone. This is $ 4,750the mechanical unit from the Sutter MP-225. Itfeatures modular construction, lower cost, smoothmovement and low drift. Includes mounting adapterplate, rod holder, 4 inch dovetail extension, hingedheadstage mount and cable to connect manipulatorto controller.** Indicate right- or left-handed set-up when ordering44 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

ACCESSORIESMPC-200 285204 4 inch dovetail extension $ 54 285210 1 Mounting adapter plate $ 60 285RBI Rotating base for MP-285 $ 415 225RBI Rotating base for MP-225 $ 415 285300 Right angle adapter $ 90 285305 Z-axis vertical extension $ 90 285310 Z-axis horizontal extension $ 90 BR-AW Rod holding clamp for XenoWorks injectors $ 260 MP-ROD Rod holder $ 90 285HEA Hinged headstage mount $ 350MICROMANIPULATIONFor detailed information on mounting our micromanipulators, refer to theMounting Systems section or phone for assistance.1For use with MT and MD series stands and platforms, or any surface with1 inch centered holes.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM45

MP-285ROBOTIC MICROMANIPULATORMICROMANIPULATION(Shown with ROE input device)FEATURESMP-285 Highly stable for experiments intolerant ofpipette drift Sub-micron resolution and integratedcoarse positioning 1 inch of motorized travel on all three axes 4th axis with user-selected angle for axial drive Adjustable speed and resolution allowsoptimization for your experimental setup Programmable robotics for complex motionsequences Continuous display (in microns) of axes positions Switch between continuous or single stepmovement Absolute and relative origins Convenient Home function allowspipettes to be quickly repositioned Assignable axes permit any orientation ofthe manipulator Easy to read vacuum fluorescent display Remote computer control via serial interface Compact design easily adaptable to your setup Universal mounting system for headstageor pipette holder Optional mounting adapters (see price list)46 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The flagship in our line of precision micromanipulators, the motorized MP-285 is affordable yet offersadvanced features found in manipulators costing thousands more. Custom engineered stepping motors,precision cross-roller bearing slides and proprietary worm gear capstan drives form the basis of thewatch-like mechanical system. The controller provides power to the stage motors with a quiet linearpower supply to minimize electrical noise radiation in your setup. Pipette holders and headstages aresecurely mounted to the MP-285 with one of our several unique and rigid mounting systems.The MP-285 was designed to meet a wide variety of positioning needs for the scientific communityand is suitable for patch clamp experiments, extracellular recording, microinjection, intra-cellularrecording and precision robotic positioning applications. An outstanding feature of this system isthe unique definable 4th axis for diagonal advancement of the pipette. You select the angle, thenactivate the 4th axis. As with the other three axes, you may move with adjustable coarse or ultrafineresolution, select the movement speed, and move continuously or in single step increments. To quicklyreposition the pipette, simply select the Home function. Axes positions are continuously shown inrelative and absolute scales, and are easily readable on the vacuum fluorescent display.MICROMANIPULATIONThe extremely low backlash of the MP-285 removes traditional drawbacks of “open loop” technologyand eliminates drift. This allows submicron resolution down to 0.2 microns in the coarse range anddown to 40 nanometers in the fine range. With over 1 inch of motorized travel on all three axes, and auser designated 4th axis, the MP-285 allows tremendous range of motion while maximizing resolution.Available with a table-top or rack mounted controller, our manipulator fi ts in seamlessly with yourother components while the compact design and assignable axes of the MP-285 allows you to easilyintegrate it into your setup at any orientation. To add to its practicality, your choice of one of twomanual controls: joystick or rotary optical encoder (ROE), assures a comfortable experimentationenvironment, customized to the scientist.For users who require repeatable motion sequences, the MP-285 features easily programmedrobotic control from the keypad, or via a remote computer. The system can store up to 500 positioninstructions, including pauses, and will execute the instruction set once, continuously, or in reverse.As always, our technical support team is available to address your concerns and answer all questionsbefore, and after your purchase.CHOOSE ONE OF TWO INPUT DEVICE OPTIONS1) Rotary Optical Encoder (ROE). Turning one of three 2-inch knobs produces a movement along oneaxis proportional to the amount and speed of the turn. Buttons allow the activation of “4th axis”, changeof movement resolution, “home” return function, and toggle between continuous pulse movements.2) Joystick. Our joystick is a modified three-axis game controller. The degree of handle deflectiondetermines the velocity of movement of each axis. In pulse mode, a button commands themanipulator to take a single step in the axis selected by joystick deflection.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM47

SPECIFICATIONSMP-285MICROMANIPULATION Travel Resolution Maximum Speed Long Term Stability Drive Mechanism Serial Interface Dimensions Weight Electrical1 inch25mm on all three axesLow: 0.2µm/stepHigh: 0.04µm/step2.9mm/sec

US PRICES MP-285International prices are 5% higher. Prices subject to change without notice. MP-285/R Includes manipulator, rack mount controller unit, cables, $ 7,900rod holder, hinged headstage mount, 4 inch dovetailextension, mounting adapter plate, choice of input device,power cord, and manual MP-285/T Includes manipulator, table top controller unit, cables, $ 7,900rod holder, hinged headstage mount, 4 inch dovetailextension, mounting adapter plate, choice of input device,power cord, and manualMICROMANIPULATIONWhen ordering the MP-285, the following items must be specified.Please list as separate items with no cost.Choose one input deviceRotary Optical EncoderJoystickSelect the manipulator handednessRight handed setupLeft handed setup285ROE285JOYRIGHTLEFTIf you have any questions regarding the part numbering system, please contactSutter Instrument Company directly.ACCESSORIESMP-285 285204 4 inch dovetail extension $ 54 285210 1 Mounting adapter plate $ 60 285RBI Rotating base for MP-285 $ 415 285300 Right angle adapter $ 90 285305 Z-axis vertical extension $ 90 285310 Z-axis horizontal extension $ 90 BR-AW Rod holding clamp for XenoWorks injectors $ 260 MP-ROD Rod holder $ 90 285HEA Hinged headstage mount $ 350For detailed information on mounting our micromanipulators, refer to theMounting Systems section or phone for assistance.1For use with MT and MD series stands and platforms, or any surface with1 inch centered holes.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM49

MECHANICAL DRAWINGS MP-285MICROMANIPULATION50 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

MECHANICAL DRAWINGS OPTIONAL ROTATING BASE (285RBI OR 225RBI)MICROMANIPULATIONPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM51

MICROMANIPULATIONMP-265/MPC-365NARROW FORMATMANIPULATOR SYSTEMFEATURESMP-265/MPC-365 Single controller and ROE will run twostepper motor drive manipulators Robotic Home and Work position moves foreasy automated pipette exchange Self-detecting, daisy-chain capabilityallows control of four manipulators fromthe ROE-200 User-friendly interface: single button accessto all major functions New Accelerated Mode for fast, manualmanipulator movement Easy toggle selection of Mode(speed/resolution, pulsed diagonal,Accelerated Mode) Display indicates X, Y, Z coordinates, Mode,active manipulator Faster robotic moves than previous versions Definable 4th axis for coaxial pipettemovement, angle selected by DIP switches Simple USB interface Toggle switch selects which manipulator isconnected to input device LED and display indicate active manipulator Low-drift mechanical stability Narrow stand with linear slide52 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Our new MP-265/M “narrow format” mechanical is designed for patch-slice manipulator systemsthat require more than 2 or 3 pipettes as well as for other setups where space is limited.The MP-265/M mechanical builds on our existing manipulator line in several ways: First, thenarrow format means more manipulators can be grouped around a recording chamber. Furthermore,the narrow format MP-265/M allows a high precision manipulator to be inserted into smallspaces in existing setups. Next, a renown Sutter, stepper-motor X-axis is coupled to a 150mmsmooth-travel, linear slide to provide both precision as well as easy approach to and from theoptical center. Finally, travel in the Y-axis is shortened (12.5 mm) both to economize on widthand because radially oriented manipulators do not require long travel in the transverse axis.MICROMANIPULATIONThe MP-265/M manipulator was designed specifi cally for systems requiring 4 or moremanipulators. To this end, we have put together systems with special pricing for 4, 5 and 6manipulators, and 2 or 3 dual manipulator controllers. For the first time, pricing includes a new,narrow-format linear slide and narrow stand for mounting the MP-265/M mechanical. With the150mm throw of the linear slide, the manipulator can be quickly and smoothly moved out to alocation where there is easy access to the pipette for replacement. A narrow format version ofour classic MT-70 stand is also included; the stand is removable so that the linear slide separatelycan be used for mounting to large platform stages like the Sutter MT-78.Through the power of the popular Sutter MPC-200 dual manipulator controller and ROE-200, andone or more MP-265/M mechanicals, one forms MP-365/M systems. A single MPC-200 controllerand ROE-200 can run one or two MP-265/M manipulators. A single ROE-200, when paired withtwo MPC-200 controllers can control up to four MP-265/M manipulators. If you require moremanipulators in a single setup, you simply duplicate the smaller systems. A new firmwareversion of the MPC-200/ROE-200 automatically recognizes the MP-265/M mechanical andadjusts accordingly.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM53

SPECIFICATIONSMP-265/MPC-365MICROMANIPULATION ResolutionMinimal microstep size is62.5 nanometers per microstep.Display has single micron resolution. Maximum Speed MP-265 mechanical 3mm/sec DRIFT< 1 micron/hr Drive Mechanism Dimensions Controller 16in x 11in x 3.5in(MPC-200):ROE:41cm x 28cm x 9cm10in x 6in x 4in25cm x 15cm x 23cm Weight Controller: 6.5lb/3kgROE: 3.5lb/1.6kg Electrical115/230 Volts50/60 Hertz power lineRoHS CompliantMECHANICAL DRAWINGS OF THE MP-265/MPC-3651.704.33*ALL MEASUREMENTS ARE IN INCHES3.54.7900.841.702.206.016.3001.7054 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES MP-265/MPC-365International prices are 5% higher. Prices subject to change without notice. MPC-365 One MP-265/M manipulator mechanical, one stand, $ 10,000one MPC-200 controller and one ROE-200,mounting adapter plate, rod holder, 4 inch dovetailextension, manipulator and ROE connecting cables,power cord and manual.* MPC-365-2 Same as the MPC-365, but with two MP-265/M $ 15,900mechanicals, two stands.* MPC-365-3 Same as the MPC-365, but with three MP-265/M $ 23,900mechanicals, three stands, and two MPC-200controllers.* MPC-365-4 Same as the MPC-365, but with four MP-265/M $ 29,000MP-265/M mechanicals, four stands, and twoMPC-200 controllers.* MPC-365-5 Same as the MPC-365, but with fi ve MP-265/M $ 37,500mechanicals, fi ve stands, three MPC-200 controllersand two ROE-200 user interfaces.* MPC-365-6 Same as the MPC-365, but with six MP-265/M $ 42,900mechanicals, six stands, three MPC-200 controllersand two ROE-200 user interfaces.*MICROMANIPULATION* Indicate right- or left-handed set-up when orderingCOMPONENTSMP-265/MPC-365 MPC-200 Controller box alone. Purchase this if you intend to $ 2,800daisy chain two controllers to one ROE-200. In thisconfiguration, up to four manipulators can be controlledby a single ROE-200. NOTE: This is not a stand-alonedevice. In order to function, the MPC-200 must beconnected to a second MPC-200 with ROE-200attached or be connected to its own ROE-200.Includes daisy chain cables, power cord and manual. ROE-200 This is the ROE alone. Provides user input to one or $ 1,250more MPC-200 controllers. This component isintended for users who have an existing fourmanipulatorMPC-200 system with a singleROE-200 and wish to split it into two independenttwo-manipulator systems. Includes cable to connectROE to controller. MP-265/M The MP-265 manipulator mechanical alone. Includes $ 6,500stand, mounting adapter plate, rod holder, 4 inchdovetail extension, hinged headstage mount andcable to connect manipulator to controller.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM55

MP-225MOTORIZED MICROMANIPULATORMICROMANIPULATIONFEATURESMP-225 Highly stable for experiments intolerant ofpipette drift Submicron (62.5nm) minimal resolution forfin e m o v e m e n t Convenient thumbwheel selects resolution/speed of movement 25mm of motorized travel on all three axes 4th axis for coaxial movement of pipette,angle selected by DIP switches on ROE ROE button press actuates move to Homeposition for pipette exchanger ROE button press actuates move toWork position near recording location Continuous display (in microns) of axespositions located on ROE DIP switches on ROE select direction ofmovement produced by turn of ROE knob Modularized, compact design easilyadaptable to your setup Universal mounting system for headstage orpipette holder Mounting adapters included with manipulator56 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The MP-225 is one of the newer products in the Sutter Instrument micromanipulator line.Production and design changes have allowed us to produce a motorized manipulator that is amore affordable alternative to the industry standard MP-285. While the MP-225 feature set isless comprehensive than the MP-285, it includes the most popular features with a simpler userinterface. The mechanical design utilizes a miniature stepper motor and integral anti-backlashgear head. Pre-loaded ball bearing slides provide smooth movement throughout the 25mm oftravel. The controller uses low-noise, linear-drive output circuitry identical to that found in theMP-285. The methodology for mounting pipette holders and headstages used with the MP-285has been maintained in the MP-225 to allow for cross compatibility.MICROMANIPULATIONThe MP-225 is designed primarily for positioning patch and intracellular recording pipettes.We have retained and refined the features most desired for this type of work. An extendedversion of the popular rotary optical encoder (ROE) is the sole input device available with theMP-225. Like the MP-285, the manipulator has a synthetic 4 th axis for diagonal advancement ofthe pipette; 16 different angles are selectable via DIP switches. Speed and resolution of movementare easily selected with a multiple position switch, allowing fast/coarse movement and slow/ultrafinemovement in 10 increments. Two commonly used robotic movements have been incorporatedfor user convenience. A single button press can initiate a move to a Home position for pipetteexchange or to a user defined Work position for quick location of the pipette near the recordinglocation. A display on the ROE gives position location. As all controls are located on the ROE, thecontroller can be moved to a less accessible area of your setup and does not need to occupy primespace in an equipment rack.As always, our technical support team is available to address your particular needs and answerall questions before and after your purchase.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM57

SPECIFICATIONSMP-225MICROMANIPULATION Travel1 inch25mm on all three axes Resolution Six microstep sizes selectable (µm/ustep): 0.0625, 0.125, 0.25, 0.5,1.0 and 2.0. Finer movement settings use the 62.5nm microstep size butfewer microsteps are commanded per encoder knob turn Maximum Speed 2.0mm/sec Long Term Stability 1-2 µm/hour maximum Drive Mechanism Integral miniature stepper motor anti-backlash gearhead Dimensions Mechanical: Controller:4in x5.5in x 6in 4in x 16in x 12.25in10cm x 15cm x 15.5cm 10cm x 40.5cm x 31cm Weight Manipulator: Controller:2.95lbs /1.3kg 10lb 11oz/4.5kg Electrical115/230 Volts50/60 Hertz power lineRoHS CompliantUS PRICES MP-225International prices are 5% higher. Prices subject to change without notice.MOTORIZED MICROMANIPULATOR MP-225 Includes manipulator, table top controller unit, extended $ 6,500ROE, hinged headstage mount, cables, rod holder, 4indovetail extension, mounting adapter plate, screws,hex wrench, power cord and manual** Indicate right- or left-handed set-up when ordering.ACCESSORIESMP-225 285204 4 inch dovetail extension $ 54 285210 1 Mounting adapter plate $ 60 285RBI Rotating base for MP-285 $ 415 285300 Right angle adapter $ 90 285305 Z-axis vertical extension $ 90 285310 Z-axis horizontal extension $ 90 BR-AW Rod holding clamp for XenoWorks ® injectors $ 260 MP-ROD Rod holder $ 90 285HEA Hinged headstage mount $ 350For detailed information on mounting our micromanipulators, refer to theMounting Systems section or phone for assistance.1For use with MT and MD series stands and platforms, or any surface with1 inch centered holes.58 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

MICROMANIPULATOR COMPARISON CHARTFeatures MPC-385/325 MPC-365 MP-285 MP-225Manipulator per Controller 2 2 1 1Manipulator per ROE 4 4 1 1Memory Moves500 and home 2 (Home and Work) Travel25mm on all three axes 25mm on X and Z axes12.5mm on Y axisInterface USB USB Serial NoneExpandable Yes Yes No NoStep Size 62.5nm 62.5nm 40nm and 200nm 62.5nmSelectable Speeds 6 6 14 14Maximum Speed5mm/sec5mm/sec (w/MP-285 manipulator) 3mm/sec3mm/sec (w/MP-225 manipulator) 2.9mm/sec2mm/secType of BearingsCross Roller Cross Roller (w/MP-285 manipulator) Ball BearingBall Bearing (w/MP-225 manipulator) Price 1 $8,500 (MPC-385) $10,000 $7,900 $6,500$7,400 (MPC-325)MICROMANIPULATION1. Price for a single manipulator, controller, and input device. U.S. prices. International prices are 5% higherPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM59

MICROMANIPULATIONMP-85HUXLEY-WALL STYLEMICROMANIPULATOR(Shown with optional spacer, wedge and magnetic feet)FEATURESMP-85 Dual springing of the moment arms to insurezero backlash and zero torsion The micromanipulator is mounted on aprecision rotating base featuring a positivestop and lock The coarse movement is an all cross-rollerbearing design consisting of coarse X, Y,and Z with an additional fine X motion The micromanipulator can be ordered ineither a right or left handed versionOPTIONSMP-85 Fifteen degree stackable wedges for tiltingthe manipulator One inch thick, chrome plated, solid brassriser blocks A set of three magnetic feet for increasedstability60 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The classic micromanipulator developed by Sir Andrew Huxley many years ago is still consideredby many investigators to be the finest manual micromanipulator available. The MP-85 offers theadvantage of a very large range of movement with its built-in coarse manipulator and precisesubmicron movement with the fine controls. Coarse positioning is achieved with a three axismanipulator mounted on top. The ultrafine positioning is accomplished with the large micrometersmounted on the base. These micrometers provide smooth, precise movement through a 10:1reduction mechanism.The brass and stainless steel construction of the MP-85 makes for a very heavy and solidmicromanipulator with excellent damping properties. This exceptionally stable design is ideal forpatch clamp recording, intracellular recording, or any other application that is intolerant of drift.MICROMANIPULATIONThe MP-85 is chrome-plated and anodized to prevent corrosion. The J.R. Wall designed rotatingbase is machined from solid stainless steel with a brass spindle; a thin Tefl on washer provides forthe bearing surface.Please contact Sutter Instrument for any additional information on the suitability and use of theMP-85 micromanipulator for your specifi c application.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM61

SPECIFICATIONSMP-85MICROMANIPULATION Coarse X-axis Tilt Coarse Resolution Fine X resolution Ultrafine (Huxley)Resolution Huxley Excursion Dimensions Weight0 to 45 degreesin 15 degree increments0.1mm on all axes0.01mm0.2µm~2mm maximum in each axis10in x 10in x 12in25cm x 25cm x 28cmManipulator:15lbs7kg62 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES MP-85International prices are 5% higher. Prices subject to change without notice.MICROMANIPULATOR MP-85/R Right-handed – Includes manipulator, coarse movement $ 4,500MM-33 micromanipulator, and rotating base MP-85/L Left-handed – Includes manipulator, coarse movement $ 4,500MM-33 micromanipulator, and rotating baseMICROMANIPULATIONACCESSORIESMP-85 Holder/MP85 Universal headstage adapter $ 160 RBI Rotating base for MP-85 $ 810 T363410 Magnetic feet (set of 3) $ 45 85AX200 Axopatch 200/Heka EPC-10 adapter $ 275 X850600 15 degree wedges (each) $ 90 X850700 1 inch increment spacer $ 125(No more than three recommended) X700115 MP-85 to MT stand adapter plate $ 90PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM63

MM-33MICROMANIPULATORMICROMANIPULATION(Shown: MM-33A/R)FEATURESMM-33 Compact design allows use in tightenvironments Rack and pinion drive gives stablemovement with minimal backlash Cross-loaded roller bearings for smooth,low friction movementOPTIONSMM-33 Rotating base allows horizontal rotationand vertical tilting Vertical axis lock64 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Successfully used in conjunction with our other micromanipulators, the MM-33 is availableseparately for those wishing a small, practical instrument in applications not requiring submicronaccuracy.The MM-33 is the right choice for tight environments which require maximum versatility within asmall space. The optional rotating base and tiltable X-axis provide even further fl exibility.Stainless steel cross-loaded roller bearings are designed to offer low friction and smooth linearmotion with a minimum of side play. The pre-loaded rollers are set at 90 degrees to the hardenedsteel guides insuring constant contact and accuracy. This precise rack and pinion drive gives stable,drift-free movement with minimal backlash. The controls are placed one above the otherwhich makes for less hand movement and easier positioning. The scales for all axes are calibratedin 0.10mm increments and the fine micrometer adjustment for the X-axis thrust is calibrated at0.01mm with estimates to 5µm.MICROMANIPULATIONThe optional rotating base extends the utility of the MM-33 by providing two additional rotatingplanes. The rotation in the horizontal plane (approximately 120 degrees) has a positive stop, quickrelease mechanism for fast insertion and removal of pipettes. The second rotating axis allows theMM-33 to be tilted in a vertical plane from 0 to 70 degrees. The optional tiltable X-axis allowsthe fine axis to be tilted up to 45 degrees. An optional vertical lock is also available for locking theposition of the vertical axis. These options should be ordered at the time of purchase since themodifi cations are factory installed.(Shown: MM-33)PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM65

INDIVIDUAL EXCURSIONS ARE AS FOLLOWS:X-axis coarse movement: 37mmMICROMANIPULATIONX-axis fine movement: 10mmY-axis horizontal movement: 20mmZ-axis vertical movement: 20mmUS PRICES MM-33International prices are 5% higher. Prices subject to change without notice.MICROMANIPULATOR MM-33/R Right-handed micromanipulator $ 995(with 1/2 inch rod clamps*) MM-33/L Left-handed micromanipulator $ 995(with 1/2 inch rod clamps*) MM-33A/R Right-handed micromanipulator with tilting mount $ 1,695for fi ne X-axis, vertical lock, rotating base, and twobase clamps MM-33A/L Left-handed micromanipulator with tilting mount $ 1,695for fi ne X-axis, vertical lock, rotating base, and twobase clamps*12mm rod clamps available upon requestACCESSORIESMM-33 MM-33000 1 Right-handed tilting mount for fine X-axis $ 150 MM-33001 1 Left-handed tilting mount for fine X-axis $ 150 MM-33002 Rotating base with two base clamps $ 500 MM-33006 Rod mounting bracket for use with stands $ 305 T363420 Magnetic feet for rotating base (set of 3) $ 45 X850447 1 Vertical lock $ 150 X330105 Base clamps (2) for clamping rotating base $ 40 X330103 1 Stationary mounting base $ 1051Must be ordered with the MM-33 since this option is factory installed only.66 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

MICROMANIPULATIONPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM67

MICROMANIPULATION3DMS3-DIMENSIONALMOTORIZED STAGE(Shown: 3DMS-200, chamber not included)FEATURES3DMS Highly stable for experiments intolerant ofpipette drift Sub-micron resolution and integratedcoarse positioning 1 inch of motorized travel on all three axes Adjustable speed and resolution allowsoptimization for your experimental setup When used with MP-285 controller,programmable robotics for complex motionsequences Continuous display (in microns) of axespositions Switch between continuous or single stepmovement Absolute and relative origins Remote computer control Compact design easily adaptable to yoursetup68 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Many imaging experiments require the ability to move to multiple locations in a chamber or in atissue culture dish. Large motorized stages accomplish this, but generally make the microscopeinto a single-use, motorized imaging station. The form factor of motorized stages usually precludespositioning other equipment such as manipulators at the same microscope.Now, a simple attachment converts a standard Sutter MP-285 manipulator into the 3-DimensionalMotorized Stage. The 3DMS manipulator and stage attachment can be either mounted on anMT-75 gantry stand next to an upright microscope, or on an MD series stand attached to thebody of an inverted microscope. The compact size allows manipulators and other equipment tobe used simultaneously.MICROMANIPULATIONRefocusing is often required at the different X-Y locations when images are automaticallyacquired. Normally this requires a motorized focus drive to be added to the microscope. Becausethe 3DMS is a three-axis manipulator, focusing (vertical movement of the stage) is already built in.Surprisingly, this relatively small package still allows for 25mm of movement in X, Y and Zaxes with sub-micron precision.The stage shown carries a Warner Instruments’ RC style chamber and PM-1 holder (not included).Other chambers can be accommodated. Please consult our technical staff for further information.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM69

SPECIFICATIONS3DMSMICROMANIPULATION Travel Resolution (withMP-285 Controller) Maximum Speed Long Term Stability Drive Mechanism Serial Interface Dimensions Weight Electrical1 inch25mm on all three axesLow: 0.2µm/stepHigh: 0.04µm/step2.9mm/sec

US PRICES 3DMS SYSTEMSInternational prices are 5% higher. Prices subject to change without notice. 3DMS-285 Includes rack mount MP-285 controller, 3-axis $ 8,400motorized stage for microscope and ROE user interface 3DMS-200 Includes rack mount MPC-200 controller, 3-axis $ 9,000motorized stage for microscope and ROE user interfaceCOMPONENTS3DMSMICROMANIPULATION 3DMS 3-axis motorized stage mechanical only $ 6,000 285DMS Stage bracket mount (converts MP-285 to stage) $ 500PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM71

MICROMANIPULATIONMT-2000MOTORIZED X-Y TRANSLATION SYSTEMFOR FIXED-STAGE MICROSCOPES(shown: MT-800/Y51)FEATURESMT-2000 22mm of travel in X & Y 40nm resolution displayed in submicrons(MP-285) 62.5nm resolution displayed in microns(MPC-200) Highly stable for experiments intolerantof drift Adjustable speed and resolution allowsoptimization for your experimental setup Programmable robotics for complex motionsequences (MP-285) Continuous display of axes positions Convenient Home function allowsmicroscope to be quickly repositioned Easy to read vacuum fluorescent display72 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The MT-2000/MT-2078 is a motorized microscope translator system including multipleplatforms for manipulators and specimen. Two general styles are available. In the classicdesign (MT-2000), an independent stand, the MT-75 is used for each manipulator and a similarstand, the MT-150 holds the tissue chamber. A new style (MT-2078) uses a single large, fi xedstage, the MT-78-FS, to support multiple manipulators and the sample. The classic MT-2000features free-standing columns that provide rock solid mounting for the tissue recordingchamber and multiple micromanipulators. Many prefer this methodology as it maximizesworking room for adjusting the microscope and changing pipettes. Furthermore, the independentstand design minimizes movement-related cross talk between manipulators. The MT-2078single stage has the advantage that it is easy to add additional manipulators and other devicesinto the design as the manipulator/chamber support is one large platform.MICROMANIPULATIONThe core of the MT-2000/MT-2078 system is the MT-800 stepper-motor driven X-Y translationtable. The motors can be driven by two-axis versions of either the popular MP-285 manipulatorcontroller or the dual manipulator controller MPC-200-ROE. A two-axis rotary optical encoder(ROE) accepts user input to the motorized translator and can be placed in any convenient locationin your setup. Either controller gives the user smooth, high-resolution manual control of motion.The MP-285 controller offers extensive on-board robotic functionality and a serial computerinterface for PC control. The MPC-200-ROE controller offers a USB computer interface for PCcontrol. Systems using the MP-285 controller are called MT-2000 or MT-2078 while systemsusing the MPC-200-ROE controller are designated MT-2200 or MT-2278.Our controller design has allowed Sutter to easily add a focus drive to the MT-2000/MT-2200/MT-2078/MT-2278 systems. The Sutter focus drive employs a direct-coupled stepper motor forlock-step focus movement and adjustable end of travel sensors to limit the travel of the microscopeobjective to a safe range for the setup. In the focus drive version, MT-2000/FD, the ROE inputdevice has inputs for X, Y and Z focus.MT-800 motorized translators are currently available for the Olympus BX-51WI, Nikon FN1,Zeiss Axioskop 2 FS and the Leica DMLFS. Focus drives are available for the Olympus BX51WI.Other microscopes can be supported. The MT-75, MT-150 and MT-78 stands and stages arecompatible with all scopes.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM73

SPECIFICATIONSMT-2000 SYSTEMSMICROMANIPULATION BaseplateDimensions Maximum Travel Minimal MicrostepSize Minimal DisplayResolution Maximum Load Weight MechanicalController8in x 15.25in x 2.25in200mm x 375mm x 55mm22-23mm50nm (MP-285 controller)78-125nm (MPC-200 controller)50nm (MP-285 controller)1 micron (MPC-200 controller)110lbs50kg35 lbs16kg11 lbs4.5KgRoHS Compliant74 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES MT-2000 SYSTEMSInternational prices are 5% higher. Prices subject to change without notice.WITH MT-75 MANIPULATOR STANDSMT-2000Includes the MT-800 motorized X-Y translator, MP-285 controllerand ROE, MT-150 chamber column, two MT-75 micromanipulatorcolumns (without linear slide), cables and manual MT-2000/Y51 1 MT-2000 for the Olympus BX51WI $ 9,900 MT-2000/Y51/FD 1,2 MT-2000 with focus drive for the Olympus BX51WI $ 11,400 MT-2000/Z25 1 MT-2000 for the Zeiss Axioskop 2 FS $ 9,900 MT-2000/Z45 1 MT-2000 for the Zeiss Axio Examiner $ 9,900 MT-2000/N65 1 MT-2000 for the Nikon FN1 $ 9,900 MT-2000/L30 1 MT-2000 for the Leica DMLFS $ 9,900MICROMANIPULATIONMT-2200Includes the MT-800 motorized X-Y translator, MPC-200-ROEcontroller and ROE, MT-150 chamber column, two MT-75micromanipulator columns (without linear slide), cables and manual MT-2200/Y51 1 MT-2200 for the Olympus BX51WI $ 10,400 MT-2200/Y51/FD 1,2 MT-2200 with focus drive for the Olympus BX51WI $ 11,900 MT-2200/Z25 1 MT-2200 for the Zeiss Axioskop 2 FS $ 10,400 MT-2200/Z45 1 MT-2200 for the Zeiss Axio Examiner $ 10,400 MT-2200/N65 1 MT-2200 for the Nikon FN1 $ 10,400 MT-2200/L30 1 MT-2200 for the Leica DMLFS $ 10,4001Please specify chamber type when ordering (Page 81).2Please contact Sutter for availability of focus drive on other microscopemakes & models.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM75

US PRICES MT-2000 SYSTEMSInternational prices are 5% higher. Prices subject to change without notice.MICROMANIPULATIONWITH MT-78-FS FIXED STAGE PLATFORMMT-2078Includes the MT-800 motorized X-Y translator, MP-285 controllerand ROE, MT-78-FS fi xed stage platform, cables and manual MT-2078/Y51 1 MT-2078 for the Olympus BX51WI $ 10,900 MT-2078/Y51/FD 1,2 MT-2078 with focus drive for the $ 12,400Olympus BX51WI MT-2078/Z25 1 MT-2078 for the Zeiss Axioskop 2 FS $ 10,900 MT-2078/Z45 1 MT-2078 for the Zeiss Axio Examiner $ 10,900 MT-2078/N65 1 MT-2078 for the Nikon FN1 $ 10,900 MT-2078/L30 1 MT-2078 for the Leica DMLFS $ 10,900MT-2278Includes the MT-800 motorized X-Y translator, MPC-200-ROEcontroller and ROE, MT-78-FS fi xed stage platform, cables andmanual MT-2278/Y51 1 MT-2278 for the Olympus BX51WI $ 11,400 MT-2278/Y51/FD 1,2 MT-2278 with focus drive for the $ 12,900Olympus BX51WI MT-2278/Z25 1 MT-2278 for the Zeiss Axioskop 2 FS $ 11,400 MT-2278/Z45 1 MT-2278 for the Zeiss Axio Examiner $ 11,400 MT-2278/N65 1 MT-2278 for the Nikon FN1 $ 11,400 MT-2278/L30 1 MT-2278 for the Leica DMLFS $ 11,4001Please specify chamber type when ordering (Page 77).2Please contact Sutter for availability of focus drive on other microscopemakes & models.76 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES MT-2000 COMPONENTSInternational prices are 5% higher. Prices subject to change without notice.MOTORIZED X-Y TRANSLATOR MT-800/Y51 Translator for Olympus BX51WI $ 8,400 MT-800/Y51/FD Translator for BX51WI with focus drive $ 9,900 MT-800/Z25 Translator for Zeiss Axioskop 2 FS $ 8,400 MT-800/Z45 Translator for Zeiss Axio Examiner $ 8,400 MT-800/N65 Translator for Nikon FN1 $ 8,400 MT-800/L30 Translator for Leica DMLFS $ 8,400MICROMANIPULATIONCHAMBER COLUMN MT-150/W20 Chamber column for Warner Instruments $ 730Series 20 recording chamber (octagon) MT-150/W20R Chamber column for Warner Instruments $ 730Series 20 recording chamber (round) MT-150/ALA35 Chamber column for ALA 35mm chamber $ 730 MT-150/CUST Chamber column for non-standard recording chamber $ 830MANIPULATOR COLUMNS MT-75 Standard gantry stand 8.71 to 12.50 inches $ 730 MT-75S 1 Short gantry stand 6.71 to 8.00 inches $ 730 MT-75T Tall manipulator stand 11.71 to 16.50 inches $ 830 MT-75XT Extra tall gantry stand 14.71 to 18.50 inches $ 830 MT-75/LS 1 Standard gantry stand with linear slide $ 1,295 MT-75S/LS 1 Short gantry stand with linear slide $ 1,295 MT-75T/LS Tall gantry stand with linear slide $ 1,395 MT-75XT/LS Extra tall gantry stand with linear slide $ 1,395PLATFORM STAGE MT-78-FS 2 Fixed stage platform and gantry support $ 3,500MT-78 CHAMBER INSERTS X040500 3 Warner Series 20 (round) – aluminum $ 100 X040503 3 Warner Series 20 (round) – stainless steel $ 100 X040505 3 Warner Series 20 (octagon) – aluminum $ 100 X040507 3 Warner Series 20 (octagon) – stainless steel $ 100 X040510 3 50mm round – aluminum $ 1001Useful for most upright scopes and the Axiovert 25 inverted scope withlow stage plate.2Please specify chamber type when ordering.3No charge when ordered with MT-78 or MT-78-FS.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM77

MICROMANIPULATIONMT-1000/MT-1078X-Y TRANSLATION SYSTEMFOR FIXED-STAGE MICROSCOPES(Shown: MT-1000, chamber not included)FEATURESMT-1000/MT-1078 25mm travel in X & Y 5 micron resolution Open design allows easy access tospecimen and microscope Highly stable for experiments intolerantof drift Accommodates a variety of stage inserts Compatible with Nikon, Olympus, Zeiss andLeica upright and inverted microscopes78 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The MT-1000/MT-1078 is a microscope translator, fi xed-stage and manipulator platform formoving optical pathway electrophysiology. Two general styles are available. In the classicdesign (MT-1000), an independent stand is used for each manipulator and a similar stand holdsthe tissue chamber. A new style (MT-1078) uses a single large, fi xed stage that is used formultiple manipulators and for the sample as well.MT-1000: the classic modular design features free-standing columns that provide rock solidmounting for the tissue recording chamber (MT-150) and multiple micromanipulators (MT-75).The columns are positioned around a movable table that provides X-Y translation of the opticalpathway in order to view multiple locations within the chamber at high resolution. Many preferthis methodology as it maximizes working room for adjusting the microscope and changingpipettes. Furthermore, the independent stand design minimizes movement-related cross talkbetween manipulators.MICROMANIPULATIONIn the new MT-1078 translator system, the large fi xed stage MT-78-FS is employed. In thisdesign it is easy to add additional manipulators and other devices to expand a setup as themanipulator/chamber support is a single large platform. Furthermore, an available magneticstainless steel insert holds the chamber and allows the easy positioning of magneticallyattached small devices nearby.At the core of both the MT-1000 and MT-1078 translator systems is the MT-500 X-Y translationtable. It is designed to allow movement of a microscope’s optics with respect to the fi xed stage(s).This methodology was developed by electrophysiologists recording from tissue slice preparations.The translator allows visualization and/or imaging of multiple tissue locations while maintainingmultiple stable recordings from the preparation. Such a system is also useful for experimentson cells in culture where one wishes to monitor several cells not in the same field of view, e.g.recording from pre and post synaptic neurons in culture and/or imaging one cell while recordingfrom or stimulating another.Translators are available for the Olympus BX-51WI, Nikon FN1, Zeiss Axioskop 2 FS and AxioExaminer, and the Leica DMLFS as well as older fi xed stage models. Inverted microscopetranslators are available for the Olympus IX71.Various different recording chambers can be accommodated in either the MT-150 chambercolumn or the insert in the MT-78-FS large stage. Sutter currently supports the WarnerInstruments/Harvard Apparatus Series 20 chambers and the ALA 35mm chambers. Other canbe accommodated. Dovetail slides for the MT-150 or inserts for the MT-78-FS are availablewith generic openings for users who wish to develop custom chambers.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM79

SPECIFICATIONSMT-500 X-Y TRANSLATORMICROMANIPULATION BaseplateDimensions Maximum Travel Resolution Maximum Load Weight8in x 15.25in x 2.25in200mm x 375mm x 55mm1in25mm0.0002in5µm110lbs50kg35lbs16kg80 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES MT-1000 SYSTEMSInternational prices are 5% higher. Prices subject to change without notice.WITH MT-75 MANIPULATOR COLUMNSIncludes the MT-500 manual X-Y translator, MT-150 chamber column,two MT-75 micromanipulator columns (without linear slide) MT-1000/Y51 1 MT-1000 for the Olympus BX51WI $ 5,900 MT-1000/Y71 1 MT-1000 for the Olympus IX71 $ 5,900 MT-1000/Z25 1 MT-1000 for the Zeiss Axioskop 2 FS $ 5,900 MT-1000/Z45 1 MT-1000 for the Zeiss Axio Examiner $ 5,900 MT-1000/N65 1 MT-1000 for the Nikon FN1 $ 5,900 MT-1000/L30 1 MT-1000 for the Leica DMLFS $ 5,900MICROMANIPULATIONWITH MT-78-FS FIXED STAGE PLATFORMIncludes the MT-500 manual X-Y translator, and MT-78-FSfi xed stage platform MT-1078/Y51 1 MT-1078 for the Olympus BX51WI $ 6,900 MT-1078/Y71 1 MT-1078 for the Olympus IX71 $ 6,900 MT-1078/Z25 1 MT-1078 for the Zeiss Axioskop 2 FS $ 6,900 MT-1078/Z45 1 MT-1078 for the Zeiss Axio Examiner $ 6,900 MT-1078/N65 1 MT-1078 for the Nikon FN1 $ 6,900 MT-1078/L30 1 MT-1078 for the Leica DMLFS $ 6,900MT-78 CHAMBER INSERTS X040500 2 Warner Series 20 (round) – aluminum $ 100 X040503 2 Warner Series 20 (round) – stainless steel $ 100 X040505 2 Warner Series 20 (octagon) – aluminum $ 100 X040507 2 Warner Series 20 (octagon) – stainless steel $ 100 X040510 2 50mm round – aluminum $ 1001Please specify chamber type when ordering.2No charge when ordered with MT-78 or MT-78-FS.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM81

US PRICES MT-1000 COMPONENTSInternational prices are 5% higher. Prices subject to change without notice.MICROMANIPULATIONTRANSLATORS MT-500/Y50 Translator for Olympus BX50WI $ 3,900 MT-500/Y51 Translator for Olympus BX51WI $ 3,900 MT-500/Y71 Translator for Olympus IX71 $ 3,900 MT-500/N65 Translator for Nikon FN1 $ 3,900 MT-500/L30 Translator for Leica DMLFS $ 3,900 MT-500/Z20 Translator for Zeiss Axioskop 2FS $ 3,900 MT-500/Z25 Translator for Zeiss Axioskop 2 FS $ 3,900 MT-500/Z45 Translator for Zeiss Axio Examiner $ 3,900CHAMBER COLUMN MT-150/W20 Chamber column for Warner Instruments $ 730Series 20 recording chamber (octagon) MT-150/W20R Chamber column for Warner Instruments $ 730Series 20 recording chamber (round) MT-150/ALA35 Chamber column for ALA 35mm chamber $ 730 MT-150/CUST Chamber column for non-standard recording chamber $ 830MANIPULATOR COLUMNS MT-75 Standard gantry stand – 8.71 to 12.50 inches $ 730 MT-75S 1 Short gantry stand – 6.71 to 8.00 inches $ 730 MT-75T Tall manipulator stand – 11.71 to 16.50 inches $ 830 MT-75XT Extra tall gantry stand – 14.71 to 18.50 inches $ 830 MT-75/LS 1 Standard gantry stand with linear slide $ 1,295 MT-75S/LS 1 Short gantry stand with linear slide $ 1,295 MT-75T/LS Tall gantry stand with linear slide $ 1,395 MT-75XT/LS Extra tall gantry stand with linear slide $ 1,395PLATFORM STAGE MT-78-FS 1 Fixed stage platform and gantry support $ 3,5001Useful for most upright scopes and the Axiovert 25 inverted scope withlow stage plate. Please specify chamber type when ordering (Page 81).82 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

MICROMANIPULATIONPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM83

MP-78/MPC-78LARGE MOVING STAGE PLATFORMMICROMANIPULATIONFEATURESMP-78/MPC-78 Stable support and solid design 25mm motorized travel in X & Y 40nm or 62.5nm resolution (depending oncontroller) Convenient Home function allows stage tobe quickly repositioned Easy to read vacuum fluorescent display Accomodates a variety of stage inserts Compatible with Nikon, Olympus, Zeissand Leica upright and inverted microscopes MP-78 is suitable for:Multi-site, two-photon, eletrophysiologyMulti-site, inverted (or other largemicroscope) electrophysiology84 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The MP-78 Large Moving Stage is available for patch slice or in vivo experiments where themicroscope body cannot be moved to view different fields of view. This is most common intwo-photon experiments where the excitation beam path must enter a fi xed microscope. In orderto visit wide-spaced fields of view, and potentially position pipettes at wide-spaced locations,the preparation and any associated manipulators must move together on a large stage.The MP-78 was introduced at the 2007 Imaging Structure and Function in the Nervous Systemcourse at Cold Spring Harbor Labs. The stage was very well received. The MP-78 is alsoappropriate for use with inverted microscopes or other scopes that are larger and not easilytranslated.MICROMANIPULATIONThe cast aluminum top plate used in the MP-78 has an attractive shape with an extensive1/4-20 threaded hole pattern on 1-inch centers. This hole pattern is appropriate for mountingSutter MP-285 or MP-225 manipulator mechanicals using the mounting adapter plates that comewith these devices. Optional rotating bases (285RBI) are also compatible and add fl exibility.Finally, the new, MP-265/M mechanical can be mounted on its narrow linear slide.The large-stage platforms provide solid, stable support and ample space to attach manipulatorsand other instruments as needed. The rigid aluminum top plate sits firmly on two of our well-knownMT-75 gantry-style stands. These stands provide height adjustment and sturdy attachment to theuser’s vibration table. Multiple degrees of freedom accommodate a variety of microscope andinstrument arrangements.In the center of the MP-78 top plate is a 4X6 inch milled pocket that will accommodate a variety ofaluminum stage inserts, designed to secure various commercial tissue chambers or slide holders.Custom aluminum inserts can also be provided. An available magnetic stainless steel insert carriescommercial chambers or can be provided with a simple large, through hole for mounting a customchamber. The magnetic insert allows small magnetic devices (perfusion lines, ground electrodes,small manipulators) to be mounted easily near the dish.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM85

SPECIFICATIONS MPC-78LARGE MOVING STAGE PLATFORMMICROMANIPULATION BaseplateDimensions Maximum Travel Resolution Maximum Load Weight Interface8in x 15.25in x 2.25in200mm x 375mm x 55mm1in25mm0.625µm/stepDisplayed in microns110lbs50kg35 lbs16kgUSBRoHS CompliantSPECIFICATIONS MP-78LARGE MOVING STAGE PLATFORM BaseplateDimensions Maximum Travel Resolution Maximum Load Weight Interface8in x 15.25in x 2.25in200mm x 375mm x 55mm1in25mmLow: 0.2µm/stepHigh: 0.04µm/stepDisplayed in submicrons110lbs50kg35 lbs16kgRS-232, 9600 baud86 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES MP-78/MPC-78International prices are 5% higher. Prices subject to change without notice.INDIVIDUAL COMPONENTS MPC-78 1 Moving platform stage plate with motorized gantry $ 12,000supports, MP-200 controller (USB interface) andROE, cables and manual MP-78 1 Moving platform stage plate with motorized gantry $ 10,900supports, MP-285 controller (serial interface) andROE, cables and manualINDIVIDUAL COMPONENTS MPC-78/ Y51/FD 2 MPC-78 with focus drive for the Olympus BX51WI $ 13,500MICROMANIPULATION MP-78/ Y51/FD 2 MP-78 with focus drive for the Olympus BX51WI $ 12,400ACCESSORIESMP-78/MPC-78MP-78 CHAMBER INSERTS X040500 3 Warner Series 20 (round) – aluminum $ 100 X040503 3 Warner Series 20 (round) – stainless steel $ 100 X040505 3 Warner Series 20 (octagon) – aluminum $ 100 X040507 3 Warner Series 20 (octagon) – stainless steel $ 100 X040510 3 50mm round - aluminum $ 1001Please specify chamber type when ordering.2Please contact Sutter for availability on other microscope makes and models.3No charge when ordered with MP-78 or MPC-78.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM87

MT-78-FSFIXED-STAGE PLATFORMMICROMANIPULATION(Shown: MT-1078/N65)FEATURESMT-78-FS Stable support and solid design Accomodates a variety of stage inserts Compatible with Nikon, Olympus, Zeiss andLeica upright and inverted microscopes MT-78-FS version compatible with SutterMT-500 or MT-800 X-Y translators MP-78 is suitable for:Multi-site electrophysiology wherethere is a need for a single large stage Optional XY translation available(motorized or manual)88 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Designed for experiments where a large single stage is dictated, the MT-78-FS large-stageplatforms provide solid, stable support and ample space to attach manipulators and otherinstruments as needed. The rigid aluminum top plate sits firmly on two of our well-knownMT-75 gantry-style stands. These stands provide height adjustment and sturdy attachmentto the user’s vibration table. Multiple degrees of freedom accommodate a variety of microscopeand instrument arrangements.The MT-78-FS, Fixed Stage platform, paired with a Sutter Instrument XY translator (either themotorized MT-800 or manual MT-500), offers a large, single-platform solution for researchersperforming experiments requiring X-Y translation of the optical pathway in order to view multiplelocations within the specimen at high resolution. Many prefer a large stage design as it lendsitself to the easy addition of manipulators, perfusion systems and other equipment to the setup.MICROMANIPULATIONThe cast aluminum top plate used in the MT-78-FS has an attractive shape with an extensive¼-20 threaded hole pattern on 1-inch centers. This hole pattern is appropriate for mountingSutter MP-285 or MP-225 manipulator mechanicals using the mounting adapter plates thatcome with these devices. Optional rotating bases (285RBI) are also compatible and add flexibility.Finally, the new, MP265/M mechanical can be mounted on its narrow linear slide.In the center of the MT-78-FS top plate is a 4X6 inch milled pocket that will accommodate avariety of aluminum stage inserts, designed to secure various commercial tissue chambers orslide holders. Custom aluminum inserts can also be provided. An available magnetic stainlesssteel insert carries commercial chambers or can be provided with a simple large, through hole formounting a custom chamber. The magnetic insert allows small magnetic devices (perfusion lines,ground electrodes, small manipulators) to be mounted easily near the dish.The MT-78-CS translates the gantry towers to provide 12.5 mm of movement. For users whowant additional degrees of freedom and combined increase in travel in X and Y, the MT-78-CScan also be paired with a manual or motorized translator.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM89

SPECIFICATIONSMT-78-FSMICROMANIPULATION BaseplateDimensions Maximum Travel Resolution Maximum Load Weight8in x 15.25in x 2.25in200mm x 375mm x 55mm1in25mm40nm110lbs50kg35 lbs16kg90 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES MT-78-FSInternational prices are 5% higher. Prices subject to change without notice.INDIVIDUAL COMPONENTS MT-78-FS 1 Fixed platform stage plate and gantry supports $ 3,500 MT-78-CS Platform stage with 12.5mm manual micrometer $ 5,200movement and gantry supportsCOMPLETE SYSTEMSMT-1078Includes the MT-500 manual X-Y translator, and MT-78-FSfi xed stage platformMICROMANIPULATION MT-1078/Y51 1 MT-1078 for the Olympus BX51WI $ 6,900 MT-1078/Y71 1 MT-1078 for the Olympus IX71 $ 6,900 MT-1078/Z25 1 MT-1078 for the Zeiss Axioskop 2 FS $ 6,900 MT-1078/Z45 1 MT-1078 for the Zeiss Axio Examiner $ 6,900 MT-1078/N65 1 MT-1078 for the Nikon FN1 $ 6,900 MT-1078/L30 1 MT-1078 for the Leica DMLFS $ 6,900MT-2078Includes the MT-800 motorized X-Y translator,MP-285 controller and ROE, MT-78-FS fi xedstage platform, cables and manual MT-2078/Y51 1 MT-2078 for the Olympus BX51WI $ 10,900 MT-2078/Y51/FD 1 MT-2078 with focus drive for the $ 12,400Olympus BX51WI MT-2078/Z25 1 MT-2078 for the Zeiss Axioskop 2 FS $ 10,900 MT-2078/Z45 1 MT-2078 for the Zeiss Axio Examiner $ 10,900 MT-2078/N65 1 MT-2078 for the Nikon FN1 $ 10,900 MT-2078/L30 1 MT-2078 for the Leica DMLFS $ 10,900MT-2278Includes the MT-800 motorized X-Y translator,MPC-200-ROE controller and ROE, MT-78-FSfi xed stage platform, cables and manual MT-2278/Y51 1 MT-2278 for the Olympus BX51WI $ 11,400 MT-2278/Y51/FD 1 MT-2278 with focus drive for the $ 12,900Olympus BX51WI MT-2278/Z25 1 MT-2278 for the Zeiss Axioskop 2 FS $ 11,400 MT-2278/Z45 1 MT-2278 for the Zeiss Axio Examiner $ 11,400 MT-2278/N65 1 MT-2278 for the Nikon FN1 $ 11,400 MT-2278/L30 1 MT-2278 for the Leica DMLFS $ 11,4001Please specify microscope make and model, and chamber type when ordering.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM91

ACCESSORIESMT-78-FSMICROMANIPULATIONMP-78 CHAMBER INSERTS X040500 1 Warner Series 20 (round) – aluminum $ 100 X040503 1 Warner Series 20 (round) – stainless steel $ 100 X040505 1 Warner Series 20 (octagon) – aluminum $ 100 X040507 1 Warner Series 20 (octagon) – stainless steel $ 100 X040510 1 50mm round – aluminum $ 1001No charge when ordered with MT-78-FS or MT-78-CS.(MT-1078 shown with MPC-385-2)92 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

MOTORIZED MICROSCOPE STAGE ANDTRANSLATOR COMPARISON CHARTDESCRIPTIONMP-78MT-2000/20783DMS MPC-78 MT-2200/22783-dimensional motorizedstageLarge moving platform stage Microscope translator andtable mounted stands and/orfixed stage for multi-patchMICROMANIPULATIONUSESMulti-site imaging ofsamples smaller than25 X 25mmSimultaneous2-Photon Imaging andelectrophysiologyMulti-electrode recordingand imaging in slices orother preparationsFeaturesFEATURESBuilt-in Z focus drive,compact form factor,compatible with uprightor inverted microscopes,25mm travel in X, Y,and Z. Adjustable speedand resolution allowsoptimization for yourexperimental setup.Absolute and relativeorigins.Programmablerobotics for complexmotion sequences whenused with MP-285controller.Tissue sample or wholeanimal and multiplemanipulators are supportedon a large, moving stage.25mm travel in X and Yallow the user to moveto multiple sites in brainor other tissue forelectrode placement andsimultaneous 2-photonimaging. The large movingstage is also useful forinverted microscope orother applications wheremoving the microscope isnot convenient or easilyaccomplished.Travel25mm in X, Y, and Z 24mm in X and Y25mm X and Y optical trainmovement via manual ormotorized microscopetranslators. Accomplisheshigh magnifi cationmicroscopy/electrophysiologyat multiple sites in asample. Manipulatatorsand tissue sample can beon separate stands or on asingle large fi xed stage.FocusBuilt-inOption for Olympus BX51WI 1 Control ROE ROE or PC ROE or PCInterface USB USB USB(with MPC-200 controller)Price 2 $8,400 $10,900 (MP-78) $3,900 to $12,900$12,000 (MPC-78)1. Please contact Sutter for availability on other microscope makes and models2. U.S. prices. International prices are 5% higherPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM93

MT-70/MT-71STANDSMICROMANIPULATIONFEATURESMT-70/MT-71 Solid extrusion provides sturdy, stablemounting Individual components provide a variety ofmounting options Adjustable height cantilever available Accomodates a variety of special layouts Suitable for upright and inverted microscopes94 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The MT-70 series of stands are sturdy, stable, and extremely versatile for mounting componentsfor a variety of research applications. They were designed for placing our heavy Huxley-stylemicromanipulators adjacent to any microscope. We found them to be so useful and adaptablethat we also use them for mounting other items such as optical components.The stands are based on a rigid extruded aluminum tower which is available in 3 inch incrementsup to 12 inches. The tower is mounted on a sturdy 3/4in thick aluminum baseplate and then fi ttedwith a 5/16in thick aluminum mounting platform. These systems are confi gurable as fi xed heightstands or, in the MT-71 series, have an adjustable height cantilevered side platform for addedversatility. The tower can be center or edge mounted to the baseplate for accommodating variousspatial layouts. Rows of 1 inch and 25mm centered clearance holes are provided in the baseplatefor attachment to tables. The mounting platforms have tapped 1/4-20 mounting holes on 1 inchcenters.MICROMANIPULATIONThe stands are available in sets, or you can order individual components separately to meet yourspecifi c requirements. Custom heights can be made to order for an additional fee. To securelyfasten our MP-85 Huxley style manipulator to the mounting plates, please order the manipulatoradapter plate at the time of purchase. Larger baseplates or mounting plates are available uponspecial order.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM95

SPECIFICATIONSMT-70/MT-71 SERIESMICROMANIPULATION BaseplateDimensions Mounting PlateDimensions Total Stand Height Weight6in x 6in x .75in153mm x 153mm x 19mm5in x 5in x 5/16in128mm x 128mm x 8mmdepending on confi guration4 1/16in to 13 1/16in104mm to 333mmdepending on confi guration3lb 11oz to 8lb 5oz1.7kg to 3.8kgMECHANICAL DRAWINGS MT-70-3MECHANICAL DRAWINGS MT-71-696 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICESMT-70/MT-71 STANDSInternational prices are 5% higher. Prices subject to change without notice.MT-70 SERIES MT-70-3 3 inch tower, baseplate, mounting $ 280platform and fastening hardware MT-70-6 Same as above with 6 inch tower $ 310 MT-70-9 Same as above with 9 inch tower $ 340 MT-70-12 Same as above with 12 inch tower $ 370(Custom tower lengths available: $20 per inch, $25 per cut)MICROMANIPULATIONMT-71 SERIES MT-71-3 3 inch tower, baseplate, mounting $ 340platform and fastening hardware MT-71-6 Same as above with 6 inch tower $ 370 MT-71-9 Same as above with 9 inch tower $ 400 MT-71-12 Same as above with 12 inch tower $ 430(Custom tower lengths available: $20 per inch, $25 per cut)ACCESSORIESMT-70/MT-71 STANDS MT-7001 Cantilever mounting assembly $ 145 T363400 Magnetic feet (set of 4) $ 60 X700095 Narrow baseplate – 3in x 6in x 3/4in thick $ 110 X700100 Baseplate – 6in x 6in x 3/4in thick $ 110 X700102 Mounting plate – 5in x 5in x 5/16in thick $ 80 X700115 MP-85 adapter plate $ 90PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM97

MICROMANIPULATIONMT-75 SERIESGANTRY STYLEMICROMANIPULATIOR STANDFEATURESMT-75 SERIES Adjustable vertical and horizontal axes Up to 360 degrees of rotation Suitable for upright and inverted microscopes Quick lock mechanism allows for easypositioning Solid construction provides stable mounting Optional linear slide provides smooth andeasy movement for pipette replacement98 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

For ultimate fl exibility in positioning micromanipulators or other devices adjacent to yourmicroscope, look to the MT-75 series stands. These unique gantry systems offer adjustablevertical and horizontal axes and up to 360 degrees of rotation. Your manipulator or other devicewill mount to the 3/4 inch thick aluminum cantilever which is dovetailed for secure positioning.The cantilever assembly is mounted on a heavy 2 inch diameter stainless steel post. The post issupported within the same extruded aluminum tower used in our MT-70 stands. The tower can becenter mounted or edge mounted to the baseplate for an additional degree of positioning freedom.Vertical positioning is achieved with an aluminum collar which securely locks onto the steel postto fi x the height of the system. The MT-75 is suitable for use with most inverted microscopes, andcan be adjusted from a minimum height of 8 5/8 inches to a maximum height of 12 1/2 inches.The MT-75S is suitable for most upright scopes, and can be adjusted from 6 5/8in to 9 1/2in.These systems are very stiff, defl ecting approximately 2µm per 25mm rise with a 1.5 kg load atthe end of the cantilever.MICROMANIPULATIONA quick lock mechanism allows easy unlocking and rotation of the post/cantilever assembly upto 320 degrees, then rotation back to the same positive stop position and locking in place (e.g.insertion and removal of microelectrodes from headstages). Remove the positive stop and youhave a full 360 degree rotation. A Tefl on washer contributes to the smooth rotation of the system.The solid aluminum baseplate has through-holes on 1inch (25mm) centers for mounting onto tables.The cantilever has eighteen 1 inch spaced 10-32 holes along the edges. The MT-75/LS manipulatormount provides additional fl exibility of movement. In multiple manipulator setups, rotationalmovement for pipette replacement is often not possible. Using a long linear rail, the manipulatorcan be quickly and smoothly moved to a location where there is easy access to the pipette forreplacement. After replacement, the manipulator can be slid back into a fi xed stop that definesthe working position.The linear slide system can also be added to existing MT-75 stands by ordering the MT-7004 linearslide cantilever alone.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM99

SPECIFICATIONSMT-75 SERIESMICROMANIPULATION BaseplateDimensions CantileverDimensions Weight6in x 6in x .75in153mm x 153mm x 19mm2.5in x 8in x .75in64mm x 204mm x 19mmMT-75:11lb 7oz(5.2kg)MT-75S:9lb 14oz(4.5kg)100 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICESMICROMANIPULATOR STANDSInternational prices are 5% higher. Prices subject to change without notice.MT-75 SERIES MT-75 Standard gantry stand 8.71 to 12.50 inches $ 730 MT-75S 1 Short gantry stand 6.71 to 8.00 inches $ 730 MT-75T Tall manipulator stand 11.71 to 16.50 inches $ 830 MT-75XT Extra tall gantry stand 14.71 to 18.50 inches $ 830 MT-75/LS 1 Standard gantry stand with linear slide $ 1,295 MT-75S/LS 1 Short gantry stand with linear slide $ 1,295 MT-75T/LS Tall gantry stand with linear slide $ 1,395 MT-75XT/LS Extra tall gantry stand with linear slide $ 1,395MICROMANIPULATION1Useful for most upright scopes and the Axiovert 25 inverted scope with low stage plate.ACCESSORIESMICROMANIPULATOR STANDS MT-7004 Linear slide cantilever (no stand) $ 700 T363400 Magnetic feet (set of 4) $ 60 X700095 Narrow baseplate—3in x 6in x 3/4in thick $ 110 X700100 Baseplate—6in x 6in x 3/4in thick $ 110PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM101

MECHANICAL DRAWINGS MT-75MICROMANIPULATION102 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

MECHANICAL DRAWINGS MT-75SMICROMANIPULATIONPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM103

MICROMANIPULATIONMD SERIESGANTRY STYLEMICROMANIPULATIOR PLATFORMSFEATURESMD SERIES Creates a stable matrix of 1/4 - 20 holes onone or both sides of a mechanical stage Open design allows easy access tospecimen and microscope Independent platform separate fromspecimen Allows for fl exible mounting of all Suttermotorized manipulators Mounts directly on microscope, preservingvaluable optical bench space104 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

For those who wish to use the MP-285 or MP-225 manipulator in conjunction with an invertedmicroscope, Sutter Instrument has designed a series of Microscope Dependent (MD) stands.The typical MD stand is a manipulator platform that bolts directly to the frame of an invertedmicroscope. Stands are available in either single-sided or double-sided versions for the mostcommon inverted microscopes made by Nikon, Olympus, Leica, and Zeiss. These platformslend themselves to the absolutely drift-free recording confi guration required when using themanipulators to position patch-electrodes to record from attached cells in culture. To mount theMP-285 to an MD stand, you also need either the X285210 mounting adapter plate (for orthogonalmounting), or the 285RBI rotating base that allows for non-orthogonal mounting and rotation ofthe entire manipulator (note: the X285210 mounting plate is included with each MP-285 andMP-225). New mounting options and adapters for new microscopes are being added frequently.Please see our web site for new additions, current mechanical drawings, and dimensions.MICROMANIPULATIONPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM105

MECHANICAL DRAWINGS MD-SERIESMICROMANIPULATIONUS PRICES MD-SERIESInternational prices are 5% higher. Prices subject to change without notice.The last digit of the MD part number identifies a single sided platform (-1),or a double sided platform (-2).ZEISSMD-90 SERIESMD-90-1 Axiovert 100/135 $ 456MD-90-2 $ 912MD-91-1 Axiovert 200 $ 456MD-91-2 $ 912MD-92-1 Axiovert 25 $ 456MD-92-2 $ 912106 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES MD-SERIESInternational prices are 5% higher. Prices subject to change without notice.The last digit of the MD part number identifies a single sided platform (-1),or a double sided platform (-2), and left (L) or right (R).NIKONMD-50 SERIESMD-50-1 Nikon Diaphot 200/300 and Nikon TE 200/300 $ 456MD-50-2 $ 912MD-51-1 Nikon TMD $ 456MD-51-2 $ 912MD-52-1 Nikon TE-2000 $ 456MD-52-2 $ 912MD-52-1/UP With Stage Up $ 456MD-52-2/UP $ 912MD-53-1 Nikon TS-100 $ 456MD-53-2 $ 912MD-54-1/L Nikon Ti $ 456MD-54-1/R $ 456MD-54-2 $ 912MD-54-1/UP With Stage Up $ 456MD-54-1/M Motorized $ 456MD-54-1/MUP Motorized with Stage Up $ 456MD-54-2/UP With Stage Up $ 912MD-54-2/M Motorized $ 912MD-54-2/MUP Motorized with Stage Up $ 912MICROMANIPULATIONLEICAMD-60 SERIESMD-60-1/L Leica DMIRB $ 456MD-60-1/R $ 456MD-60-2 $ 912MD-61-1 Leica DMIL $ 456MD-61-2 $ 912MD-62-1/L Leica DMI 4000/5000/6000 $ 456MD-62-1/R $ 456MD-62-2 $ 912OLYMPUSMD-80 SERIESMD-80-1 Olympus IX 50/70 $ 456MD-80-2 $ 912MD-81-1 Olympus IX 51/71/81 $ 456MD-81-2 $ 912PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM107

OPTICAL INSTRUMENTSPrecise mechanical movement and wellengineered electronics have always beenhallmarks of Sutter Instrument products. Overfi fteen years ago, our entrance into the opticalproducts market capitalized on these attributeswhen we set out to produce a reliable, fast,electronically quiet, computer controlled filterwheel. The result was the Lambda 10, whichmet all of our original performance goals, andproved itself as a very rugged and reliableinstrument.The success of the Lambda 10 led to thedevelopment of a new generation of wavelengthswitching products, and one of the widestranges of accessories in the industry. Ourcurrent design, the Lambda 10-3 controllerallows for the use of three filter wheels withoutthe expense of additional controllers. TheLambda 10-B has also been added as a lowercost alternative for those who do not require allthe features of the Lambda 10-3. Both of thesecontrollers can be coupled to our original filterwheel, as well as our latest line of wheels whichhave been designed for 25mm as well as largerdiameter filters.When the Lambda 10 was first introduced,most imaging systems could not keep up withits 55msec switching time. As technology hasadvanced in the field of imaging, demand hasincreased for faster wavelength switchingspeed. Sutter Instrument has respondedwith the Lambda DG-4 PLUS , an integratedillumination system capable of switchingwavelengths in less than 0.5msec.108 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

As demands for high throughput and lowerexposure times have increased, the need for astand-alone high power light source was metwith the Lambda LS. This integrated 175 (or 300)Watt xenon arc lamp, cold mirror, and powersupply system is available in an effi cient singlecabinet design. Our lamp product family wasrecently expanded with the Lambda XL, whichuses electrodeless bulb technology to producea highly stable, long lifetime light source.Sutter Instrument developed and designed theSmart Shutter to address the need for a robustand reliable shutter to serve as a component inour imaging line or as a stand-alone solution foroptical applications. The Smart Shutter offersthe most sophisticated shutter control available.Microprocessor control of the stepper-motorallows the user to optimize movement for speedor smoothness, produce a variable aperture, andachieve open/close times of 8msec fromtrigger. The Smart Shutter is compatiblewith the Lambda 10-3, Lambda 10-B/IQ, andLambda SC controllers.Customization of our optical product line forunique applications has become a specialtyfor Sutter Instrument. New technology hasbeen incorporated in various custom and OEMfilterwheels. For example, for systems using alarge number of filter wheels, Sutter Instrumentdeveloped an RS-485 serial bus to allow upto 16 controllers to share a single serial porton a host computer. Sutter Instrument hasalso developed sensor and motor technologyfor a system of filter wheels running at liquidhelium temperatures for use with astronomicaltelescopes. Please contact us directly formore information about custom filter changingdevices.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM109

MOMMOVABLE OBJECTIVE MICROSCOPENEWOPTICAL INSTRUMENTS(Objective not included)FEATURESMOM Objective moves 22mm in X, Y and Z Objective rotates about optical axis forimaging of non-horizontal surfacesand volumes Cambridge Technology 6210 XY scannerswith 3mm dielectric mirrors and Micromax673 dual axis controllers Two channel detector system withHamamatsu R6357 PMTs and C7319preamplifiers Sutter PS-2 dual channel PMT power supply National Instruments NI-6110 based dataacquisition system110 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The Movable Objective Microscope (MOM) is the basis of an in vivo, two-photon microscope,particularly when 2-P imaging requires simultaneous electrophysiology. The objective moves inthree dimensions, and can tilt, allowing for easy approach to brain areas that are not horizontal;in vivo specimens can be fi xed in a more natural position. Sutter offers a package that includes,in addition to the central core of the scope: XY scanners, photomultipliers, power supplies anddata acquisition.The MOM is an ultra-stable, versatile, fluorescence platform for electrophysiology experiments.As such, the MOM is also a useful alternative to the popular technique employing a fi xed-stage,upright microscope on a translating X-Y table. The advantage of the MOM is that only themicroscope objective is moving in three dimensions. The main frame of the microscope, includingthe scanners, laser, wide field excitation and camera mount are firmly bolted to the tabletop viaa rock solid aluminum extrusion.OPTICAL INSTRUMENTSThe Moving Objective Microscope body is very compact; it allows easy access for multiplemanipulators. One can place the specimen on a simple stand, like the Sutter MT-150 or stereotaxicframe and closely appose a number of other devices. Because there is no microscope frame belowthe objective, it is comparatively easy to use this microscope for larger in vivo preparations.Finally, the MOM is easily convertible from an upright to an inverted microscope. This would allowfor fi xed-stage electrophysiology experiments on cells in culture. Current designs require a massivetranslator and fi xed stage in combination with a commercial inverted scope.APPLICATIONS- In vivo two photon microscopy- Patch-slice, fi xed-stage microscopy- Non-horizontal surface microscopy- Simultaneous retainal stimulation and two photon microscopy 11“Eyecup scope-optical recordings of light stimulus-evoked fl uorescence signals in the retina”,Euler et al, Pfl ugers Arch, 2008PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM111

SPECIFICATIONSMOM AND CONTROLLEROPTICAL INSTRUMENTS Travel Resolution Maximum Speed Long Term Stability Drive Mechanism Serial Interface Electrical1 inch25mm on all three axesLow: 0.2µm/stepHigh: 0.04µm/step2.9mm/sec

US PRICES MOMInternational prices are 5% higher. Prices subject to change without notice. MOM-3MM MOM for 2-P microscopy Please contact SUTTERfor price quotes.Includes Moving Objective Microscope, 2 channel detector with PMTs, preamps andPS-2 Power supply, 3mm XY scanners with servo drive, wide field fluorescence unitincluding vertical illuminator, LB-LS/OF30 Xenon Arc lamp, LLG and light guideadapter, C-mount for wide field camera, Data Acquisition systemDoes not include 2P laser, objective, table mount optics for routing laser into scope.Compatible with most major microscope objectives.OPTICAL INSTRUMENTSPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM113

OPTICAL INSTRUMENTSLAMBDA DG-4/DG-5 PLUSHIGH SPEEDWAVELENGTH SWITCHERNEWFEATUREFEATURESLAMBDA DG-4/DG-5 PLUS Complete system for wavelength switching Integral shuttering Integral neutral density filtering Two outputs for monitoring filter position Turbo blanking Strobe-pulsed ring buffer control Direct computer control via parallelor serial interface Video sync pulsed ring buffer Video sync with strobe low PLUS version offers 30% more output power Switches in 0.5msec114 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The Lambda DG-4/DG-5 PLUS is a complete illumination system offering speed and versatility forexperiments requiring rapid wavelength switching. New digital servo technology allows faster filterswitching and 30% greater light output than the first generation DG-4/DG-5. The instrument retainsall the advantages of interference filter based systems, yet eliminates the temporal constraintsimposed by traditional filter changing devices like filter wheels. Switching between any twowavelengths is achieved in 0.5msec, allowing the user to perform real-time video imaging. The highswitching speed of the Lambda DG-4/DG-5 PLUS facilitates the ability to follow fast changes inion concentrations in dual wavelength ratio imaging applications and to monitor other concomitantchanges in the studied system at additional wavelengths.OPTICAL INSTRUMENTSNarrow bandpass systems, such as single cavity interference filters, grating monochromators,and A.O. modulators, pass unwanted harmonics of the desired wavelength. Also, with variablewavelength devices, it is not always possible to obtain suffi cient blocking of out-of-bandwavelengths. Modern interference filters, as used in the Lambda DG-4/DG-5 PLUS, do not passharmonics and have integral blocking characteristics 1000 times better than typical monochromatorsystems. For the same full width at half maximum (FWHM), interference filters have a narrowerspectral bandpass than monochromators due to the absence of the slit function.The dual galvanometer design of the Lambda DG-4/DG-5 PLUS allows tuning of the relativeintensities at each wavelength. This adjustment is difficult to obtain in variable wavelength deviceswith a single optical path. Unlike monochromators and other wavelength selective systems, theLambda DG-4/DG-5 PLUS can be used as a source of white light when required.How it works:This unique optical design of the Lambda DG-4/DG-5 PLUS is based on dual scanning galvanometersutilizing interference filters for wavelength selection. The light from the xenon arc lamp is focused onthe first galvanometer mirror which directs it via a parabolic mirror, through one of the interferencefilter channels. Following the filter, a second parabolic and a second galvanometer mirror collect andredirect the light into the light guide. A cold mirror in the beginning of the light path eliminates theIR radiation, reducing significantly the amount of heat absorbed by the optics and the sample. Coldmirrors modified to pass near-IR to 780 to 880nm are also available.A built-in shutter function allows reduction of light intensity by fi ve to six orders of magnitude. Forapplications requiring mechanical shuttering, like time lapse experiments or very sensitive samples,the Smart Shutter can be integrated into the device to assure that the light is completely blocked.The standard system, the Lambda DG-4 PLUS, holds up to four 25mm interference filters. TheLambda DG-5 PLUS, a fi ve filter version, accommodates three 18mm and two 25mm filters. While theswitching time between any two wavelengths is done in less than 0.5msec, the dwell time at anywavelength is arbitrarily set by the user.The liquid light guide can be coupled to the illumination port of most microscopes using an adapter whichcan be purchased separately. Please refer to the “Microscope Adapters” section for further information.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM115

SPECIFICATIONSLAMBDA DG-4/DG-5 PLUSOPTICAL INSTRUMENTS Lamp Type Output Range300 Watt ozone free xenon arc bulb330nm to 700nm - Ozone free Lamp Lifetime Expected lifetime 1,000 hours(All bulbs have a proratedwarranty for 500 hours) Power Consumption 350 Watts Filter Diameter Dimensions Light Guide Weight ElectricalDG-4: Four, 25mm (1 inch)DG-5: Two, 25mm (1 inch)and Three 18mm10in x 10in x 19in25cm x 25cm x 48cm2 meters long3mm diameter45lbs20kg115/230 Volts50/60 Hertz power lineRoHS Compliant116 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

FEATURESLAMBDA DG-4/DG-5 PLUS COMPLETE SYSTEM FORWAVELENGTH SWITCHINGThe Lambda DG-4 / DG-5 PLUS is a completeintegrated 175 (or 300) Watt light source andwavelength switching excitation system.Four or five interference filters can be easilyinstalled in the DG-4 or DG-5 PLUS, respectively.An additional standard neutral density filter can beinserted in the common path of the light.The light guide output from the Lambda DG-4 /DG-5 PLUS provides uniform spatial illumination,as well as vibration isolation from your microscopeA cold mirror assembly eliminates IR radiation,extending the lifetime of the optics and the lightguide, and preventing the exposure of the sample toIR when white light is necessary. INTEGRAL SHUTTERINGThe Lambda DG-4 / DG-5 PLUS provides a highspeed shutter function with open/close times of500µs. The shutter function reduces light intensityby 5 to 6 orders of magnitude.If complete light shuttering is required, a mechanicalshutter (i.e. Smart Shutter ) can be incorporatedinto the Lambda DG-4 / DG-5 PLUS. INTEGRAL NEUTRAL DENSITYFILTERINGNeutral density filtering is achieved by offsettingthe output galvanometer such that the light is notcentered on the liquid light guide. Up to 15 logicalfilters can be defined with this method. Due to thescrambling effect of the light guide, the output hasexcellent uniformity.Direct insertion of neutral density optical filters isalso possible in the filter holders at any of the fouroptical channels. A final neutral density optical filtercan be placed in the exiting light path which willreduce the light output from all optical channels. TWO OUTPUTS FOR MONITORINGFILTER POSITIONA 4 bit TTL signal transmits the current opticalchannel (filter) position.A digital-to-analog converter (DAC) output producesa voltage showing which filter is in use. TURBO BLANKINGThe turbo blanking feature is designed for sitiuationswhen the switching is done between non-adjacentfilter positions. When the instrument is in this mode,the input and output galvanometer mirrors moveout of sync, thus preventing the sample form beingexposed to light of unwanted wavelength duringswitching and shuttering.OPTICAL INSTRUMENTSPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM117

METHODS OF CONTROLLAMBDA DG-4/DG-5 PLUSOPTICAL INSTRUMENTS DIRECT COMPUTER CONTROL VIAPARALLEL OR SERIAL INTERFACEWhen operated in these modes, the LambdaDG-4 / DG-5 PLUS control commands are asubset of our Lambda 10-2 controller andwill operate with software written for theLambda 10-2.If you plan to control the DG-4 PLUS with otherinterfaces, please contact Sutter for specific issues. VIDEO SYNC PULSED RING BUFFERCONTROLIn this mode, a video sync pulse can be used toinitiate and/or execute the filter change for asequence of filter values stored in the ring buffer. VIDEO SYNC WITH STROBE LOWIn this mode, the system will implement afilter change on the video sync pulse, but onlyif the strobe line is held low. This allows acomputer to override the sync pulse. STROBE-PULSED RING BUFFERCONTROL(View of back panel)A sequence of up to 32 filter values can beloaded into a ring buffer via keypad or computer.The system will switch to the next filter in thebuffer on a TTL level (trigger) strobe pulse.After executing the last filter change in thestring the system resets to the first filterand continues.118 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES LAMBDA DG-4/DG-5 PLUSInternational prices are 5% higher. Prices subject to change without notice.DG-4/DG-5 PLUS BASIC SYSTEM WITH IMPROVED GALVOSIncludes main unit with 300W pre-aligned xenon arc bulb, 4 slide-in filter holders (25mm),1 neutral density filter holder (25mm), power cord, serial and parallel cables, liquid light guide,spanner wrench, and manual. (DG-5 PLUS includes an additional 18mm and fl at wrench. Threepositions are 18mm, and two are 25mm in diameter). DG-4PLUS/OF30 DG-4 Plus with 300 Watt ozone-free bulb $ 21,310 DG-5PLUS/OF30 DG-5 Plus with 300 Watt ozone-free bulb $ 21,310SPECIALTY DG-4 DG-4/T DG-4 with tungsten bulb $ 21,310OPTICAL INSTRUMENTSACCESSORIES O661176 Ozone free 175 Watt xenon bulb $ 670(useful wavelength range 330-700nm) O661301 Ozone free 300 Watt xenon bulb $ 670(useful wavelength range 330-700nm) O661175 Full spectrum 175 Watt xenon bulb $ 670(useful wavelength range 300-700nm) O661300 Full spectrum 300 Watt xenon bulb $ 670(useful wavelength range 300-700nm) O661115 Housing and heat sinks for bulb (bulb will be $ 285installed when ordered at the same time as housing) IQ25-DG 25mm SmartShutter to fi t Lambda DG-4/DG-5 $ 1,450(requires controller) DG-IF Interference filter holder (25mm) $ 95 DG-IF/18 Interference filter holder (18mm) $ 95 DG-ND Neutral density filter holder $ 95 X100160 Retaining ring (25mm) $ 16 X100150 Filter spacer (25mm) $ 16 X100120 Interference filter cup (25mm) $ 33 X664162 Neutral density filter cup (25mm) $ 33 X100158 Retaining ring (18mm) $ 16 X100148 Filter spacer (18mm) $ 16 X100118 Interference filter cup (18mm) $ 48 CMAC Serial cable for Macintosh $ 100Mounting adapters for Nikon, Zeiss, Olympus and Leica microscopesare available. Please see the Microscope Adapter list at the end of this section.Please call Sutter Instrument for pricing and further information.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM119

LAMBDA XLEXTENDED LIFE LIGHT SOURCEOPTICAL INSTRUMENTSFEATURESLAMBDA XL 10,000 hour expected lifetime* No high-voltage pulse Very stable output Built-in driver for filterwheel andSmartShutter Compact stand-alone design USB interface*Bulb carries a prorated 2-year warranty120 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The newest member of our optical product family, the Lambda XL, is a broad spectrum, highlystable light source (1% peak-to-peak fluctuations).The connection to the microscope is made through a liquid light guide, which assures outputuniformity in the field of view. Two light guide options are available, one optimized for the nearUV-visible and the other for the visible-near IR regions.The output at the end of the light guide is similar to that of our popular 300W Lambda LS xenonarc light source. The lamp module is expected to last in excess of 10,000 hours and expected tomaintain at least 50% of the original intensity.OPTICAL INSTRUMENTSThe compact, stand-alone cabinet design contains the bulb, lamp housing, power supply andoptics in a single enclosure. The new design includes also a filter wheel and SmartShutter driver,making it a versatile choice for fluorescence imaging applications. The Sutter filter wheel withSmartShutter or the stand-alone SmartShutter can be accommodated within the body of theLambda XL.The light intensity can be adjusted to different levels of attenuation. When the optional filterwheel is used, each filter position can be associated with its own programmable attenuation level,selected every time the filter is called.*Mounting adapters for Nikon, Olympus, Zeiss and Leica microscopes are available.*Patent pendingPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM121

SPECIFICATIONS LAMBDA XLOPTICAL INSTRUMENTS Output Range Lamp Expected Life Dimensions Electrical330nm to 700nm10,000 hours9.4in x 7.6in x 12.6in23.9cm x 19.3cm x 32cm120/240 Volts50/60 Hertz power lineRoHS Compliant122 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES LAMBDA XLInternational prices are 5% higher. Prices subject to change without notice.BASIC SYSTEM LB-XL Includes the Lambda XL light source, power cord, $ 6,250and manualACCESSORIES LLG/XL Liquid light guide, lens, and lens tube $ 1,600(2 meters, 3mm diameter) LLG/5XL 1 Liquid light guide, lens, and lens tube, $ 1,600(2 meters, 5mm diameter) LLG/XL380 2 Liquid light guide, lens, and lens tube, $ 1,600(2 meters, 3mm diameter) LLG/5XL380 2 Liquid light guide, lens, and lens tube, $ 1,600(2 meters, 5mm diameter) IQ25-XL 25mm SmartShutter with housing to fi t Lambda XL $ 1,450 LB10-NWIQ/XL 10 position 25mm filter wheel with SmartShutter $ 3,675for mounting in Lambda XL LB10-WHS4IQ/XL 4 position 25mm filter wheel with SmartShutter $ 3,675for mounting in Lambda XL O8000110 3 Replacement lamp and power supply $ 1,600OPTICAL INSTRUMENTSMounting adapters for Nikon, Zeiss, Leica and Olympus microscopes are available.Please see the Microscope Adapter list at the end of this section.1This lightguide offers a larger field of view but less intensity per unit area.2Allows higher output into the near IR.3Requires installation and realignment at Sutter. Labor included in price of lamp.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM123

OPTICAL INSTRUMENTSLAMBDA LSSTAND-ALONE XENON ARC LAMPAND POWER SUPPLY(Shown with optional lightguide and lens tube)FEATURESLAMBDA LS Xenon lamps provide light levels whichexceed those of standard microscopefluorescence lamps Equipped with a cold mirror to eliminate IRheating of down stream optical components Compact stand-alone lamp housing-powersupply enclosure Pre-aligned bulb eliminates commonfocusing problems Integrated hour meter for convenientmonitoring of lamp life Modular construction allows use ofoptional liquid light guide for fl exibledirection of light output Easily accommodates Sutter Instrumentfilter wheels or Smart Shutter within the body of the lamp. Can be coupled via a liquid light guide tomany standard microscopes (Nikon, Zeiss,Leica and Olympus). Microscope couplingrequires special adapters (not included).Please see the Microscope Adapter sectionin this catalog.124 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The Lambda LS is a stand-alone lighting system consisting of a xenon-arc lamp, lamp housing,cold mirror and power supply in a single enclosure. The Lambda LS is designed to be usedwith a liquid light guide which transmits remarkably fl at, intense, illumination to the opticaltrain of the user’s microscope or other instrumentation. The lamp’s cabinet accommodates astandard Sutter Instrument filter wheel that slides easily in and out of a slot in the light path.If desired, a second filterwheel can be mounted on the outside of the cabinet. When used withappropriate adapters, the light guide output is compatible with most common microscopesystems. Unlike the arc lamps used with most fluorescence microscopes, the xenon bulb ispre-aligned using a parabolic mirror and does not require alignment, focusing or collimation.In the standard confi guration, the Lambda LS bulb is capable of producing light output from330nm to a cutoff of 650nm determined by the cold mirror. An optional enhanced UV bulbproduces output much lower into the UV (cut off near 200nm). As with any UV generatinglight source, the optional bulb generates signifi cant quantities of ozone and must be used inan adequately ventilated environment.OPTICAL INSTRUMENTSThe Lambda LS utilizes a compact design, which places power supply, lamp house, arc lampand cold mirror in a single enclosure. This system eliminates a common failure associated withstandard arc lamp designs: when using a remote power supply aging may lead to a decreasedability to light the lamp due to loss of the insulating characteristics of the lengthy high-tensionline. As with all our equipment, the power supply has been designed to minimize electricalnoise that can be picked up by physiological recording equipment.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM125

SPECIFICATIONSLAMBDA LSOPTICAL INSTRUMENTS Output Range Lamp Type Radiant OutputStandard bulb 330nm to 650nm(optional full-spectrum bulb)200nm to 650nm175 or 300 Watt Xenon(pre-aligned to producecollimated output)2.5 Watts (175W lamp)4.5 Watts (300W lamp)(broadband, full beam) Lamp Life1000 hours (Bulb carries awarranty for 500 hours. Longerlife depends on application.Expected life is 1000 hours) Power Consumption 175 Watts or 300 Watts Dimensions Weight Electrical10.5in x 9.5in x 10in26.7cm x 24.1cm x 25.4cm10.5lbs4.8kg120/240 Volts50/60 Hertz power lineRoHS Compliant126 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES LAMBDA LSInternational prices are 5% higher. Prices subject to change without notice.BASIC SYSTEM LB-LS/FS17 Includes 175 Watt full spectrum lamp, $ 4,950cold mirror, power supply and lamp housing, supportbase with mounting rods, drop-in filter holder and manual LB-LS/OF17 Includes 175 Watt ozone free lamp, cold $ 4,950mirror, power supply and lamp housing, support basewith mounting rods, drop-in filter holder and manual LB-LS/FS30 Includes 300 Watt full spectrum lamp, cold $ 5,190mirror, power supply and lamp housing, support basewith mounting rods, drop-in filter holder and manual LB-LS/OF30 Includes 300 Watt ozone free lamp, cold $ 5,190mirror, power supply and lamp housing, support basewith mounting rods, drop-in filter holder and manual LB-LS/OF30R 1 Includes 300 Watt ozone free lamp, cold $ 5,190mirror that refl ects to 780, power supply and lamphousing, support base with mounting rods, drop-in filterholder and manualBULBS O661176 Ozone free 175 Watt xenon bulb $ 670(attenuated output below 340nm) O661175 Full spectrum 175 Watt xenon bulb $ 670 O661301 Ozone free 300 Watt xenon bulb $ 670(attenuated output below 340nm) O661300 Full spectrum 300 Watt xenon bulb $ 670 O661115 Housing and heat sink for bulb (bulb will be $ 285installed when ordering at same time as housing)ACCESSORIES LLG Liquid light guide and coupling adaptor $ 1,400(2 meters, 3mm dia.), C-mount, lens and lens tube LLG/380 2 Liquid light guide and coupling adaptor $ 1,400(2 meters, 3mm dia.), C-mount, lens and lens tube DROP-IN Drop-in filterholder (25mm) $ 75OPTICAL INSTRUMENTSMounting adapters for Nikon, Zeiss, Leica and Olympus microscopes areavailable. Please see the Microscope Adapter list at the end of this section.1Order with LLG/380.2Allows light input into near IRPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM127

MECHANICAL DRAWINGS LAMBDA LSOPTICAL INSTRUMENTS(There is additional depth whensecond filterwheel is attached)128 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

OPTICAL INSTRUMENTSPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM129

LAMBDA 10-3OPTICAL FILTER CHANGEROPTICAL INSTRUMENTSFEATURES LAMBDA 10-3 As fast as 40msec between adjacent filters(10 position wheel) As fast as 31msec between adjacent filters(4 position wheel) Can control three wheels and two shutters,or two wheels and three shutters Can accommodate Smart Shutter andUniblitz ® shutter 7 speed settings to optimize movement Command set compatible withLambda 10-2 Automatically detects installed hardware Serial, parallel and USB interfaces Can control a variety of 25mm, 32mm &50mm wheels Chopper drives Universal power supply130 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The Lambda 10-3 is the latest generation of Sutter’s high performance filter wheel changers. The controllernow offers faster switching times, synchronous independent control of 3 filter wheels and 2 shutters(or 2 filter wheels and 3 shutters), and expanded communication interface options. Recent advances inmotor technology allow the Lambda 10-3 to achieve switching times of 40msec between adjacent filters.In addition, the controller commands can now be accessed via USB, serial, parallel, and keypad interfaces.The Lambda 10-3 controls a variety of standard Sutter filter wheels including the popular 10 position25mm wheel, a lighter 4 position 25mm wheel, a 10 position 32mm wheel, 5 position 50mm wheel, anda compact belt-drive 4 position wheel. The embedded controllers automatically detect the equipmentinstalled and the source of the external commands, so there are no jumper wires or switches to set. Thereare seven speed settings available to optimize the rate of movement in accordance with the load of thewheel. The Lambda 10-3 can operate up to 40msec switching time with the traditional 25mm filter wheelas well as our newer, high-speed 32mm version. The 4 position wheel (LB10-NWHS4) provides additionalspeed and can achieve switching times of less than 33msec. Nearly all of our filter wheels employ a directdrive system. This design eliminates problems with belt slippage and backlash, assuring that our wheelsare exceptionally robust.OPTICAL INSTRUMENTSEach wheel will accommodate filters from 1 to 9mm in thickness, allowing multiple filters to be used ineach position. For instance, this allows users to insert a neutral density filter along with an interferencefilter in a single position. We have designed the body of the filter wheel with a trap to eliminate lightscatter. For added stability, non-emission filter wheels come with a sturdy support stand and 12 inch rods.In addition to filter wheels, the Lambda 10-3 controller supports our patented stepper-motor controlledSmart Shutter as well as the Uniblitz ® shutter. Either shutter can be mounted directly onto the wheel,providing a compact wheel/shutter package for systems with mounting constraints.Both shuttered and non-shuttered filter wheels can be installed directly on the microscope in a number oflocations: excitation, emission, Keller ports, and the trinocular head. Up to two excitation and/or neutraldensity filter wheels can be used with the Lambda LS xenon light source and liquid light guide, in vibrationsensitive systems. The Smart Shutter can be mounted independently from the wheel in any location in thelight path (including the Lambda LS and Lambda DG-4/DG-5), and driven in a stand-alone configuration bythe Lambda 10-3 controller. The microscope specific adapters necessary for any configuration of wheelsand shutter are listed in the Microscope Adapters section of the catalog.The Lambda 10-3 commands are a superset of the Lambda 10-2, thus integrating all previously availablefeatures into the new design. Additional commands are incorporated to access the third wheel as well asthe special Smart Shutter features. These configurations can be programmed through the keypad makingit easy to use the previous shutter commands to access the Smart Shutter specific functions such as theneutral density or the ‘soft’ shutter mode.Filter selection can be made directly from the keypad or from a computer via the serial, parallel, or USB port.The controller determines the shortest route to the selected filter and an acceleration/deceleration algorithmminimizes vibrations during the movement of the wheel. Internal sensors ensure correct filter positioning andcurrent filter position is displayed on the front panel. The serial port accepts RS-232 level signals through a DB-9connector. USB input is made through a standard connector and can be directly connected to a PC USB port.The universal power supply will automatically switch to accommodate the line voltage – no useradjustment is required. An optional liquid light guide is available for applications requiring absolutevibration isolation, and/or spatial illumination uniformity.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM131

SPECIFICATIONS LAMBDA 10-3OPTICAL INSTRUMENTS Control BoxDimensions Weight Electrical8.2in x 5.9in x 5.1in14.9cm x 20.8cm x 13cm20lbs11kg120/240 Volts50/60 Hertz power line75 Watts maxMECHANICAL DRAWINGS LAMBDA 10-NWIQRoHS Compliant(Shown with optional Smart Shutter )132 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES LAMBDA 10-3International prices are 5% higher. Prices subject to change without notice.CONTROLLER LB10-3 Includes one Lambda 10-3 controller unit, serial, $ 2,520parallel and USB cables, power cable and manualWHEELS25mm Filter Wheels – 10 position LB10-NW 10 position 25mm filter wheel without shutter $ 2,900 LB10-NWIQ 10 position 25mm filter wheel with SmartShutter $ 3,675 LB10-NWS 10 position 25mm filter wheel with Uniblitz ® shutter $ 3,500 LB10-NWE 10 position 25mm filter wheel set up for emission $ 2,62525mm Filter Wheels – 4 position LB10-WHS4 4 position 25mm filter wheel without shutter $ 2,950 LB10-WHS4IQ 4 position 25mm filter wheel with SmartShutter $ 3,725 LB10-WHS4E 4 position 25mm filter wheel set up for emission $ 2,95025mm Dual Filter Wheels LB10-WD Back to back mounting of two 25mm filter wheels $ 5,775without shutter. No drop-in filter holders LB10-WDIQ Back to back mounting of two 25mm filter wheels $ 6,650with SmartShutter and one slide-in filter holder LB10-WDS Back to back mounting of two 25mm filter wheels $ 6,560with one Uniblitz shutter and one slide-in filter holder32mm Filter Wheels – 10 position LB10-W32 10 position 32mm filter wheel without shutter $ 3,150 LB10-W32IQ 10 position 32mm filter wheel with SmartShutter $ 4,725 LB10-W32S 10 position 32mm filter wheel with Uniblitz shutter $ 4,37550mm Filter Wheel – 5 position LB10-W50 5 position 50mm filter wheel without shutter $ 3,675Specialty Wheels LB10-W12 10 position 12.5mm filter wheel without shutter $ 2,700 LB4-W Belt drive 4 position 25mm filter wheel without shutter $ 2,950OPTICAL INSTRUMENTSPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM133

US PRICES LAMBDA 10-3International prices are 5% higher. Prices subject to change without notice.OPTICAL INSTRUMENTSSMARTSHUTTER IQ12-SA 12.5mm SmartShutter with stand-alone housing $ 995 IQ25-SA 1 25mm SmartShutter with stand-alone housing $ 1,050 IQ25-W 2 25mm SmartShutter with housing to fit filter wheel $ 1,050 IQ25-LS 25mm SmartShutter with housing to fi t Lambda LS $ 1,100 IQ25-DG 25mm SmartShutter to fi t in Lambda DG-4/DG-5 $ 1,450 IQ35-W 35mm SmartShutter with housing to fit filter wheel $ 1,525 IQ35-SA 35mm SmartShutter with stand-alone housing $ 1,5251Where vignetting may be an issue, we recommend the 35mm shutter.2For upgrading a 25mm filter wheel with existing Uniblitz ® shutter to SmartShutter.CABLES AND ACCESSORIES CIQ-2 One 25 pin connector to two 9 pin connectors $ 100(Allows connection of one stand-alone SmartShutter to controller) SLIDE-IN 1 Slide-in filter holder for 25mm wheel $ 75 DROP-IN 2 Drop-in filter holder for 25mm wheel $ 75 DROP-IN/32 Drop-in filter holder for LB10-W32IQ $ 75 LLG Liquid light guide (2 meters, 3mm diameter) C-mount, $ 1,400lens, and lens tube SHUTTER 25mm replacement shutter for Uniblitz ® shutter $ 500(not an upgrade) W621510 15 pin shielded cable for standard filter wheel $ 28 W620009 25 pin cable for wheels with SmartShutter $ 28 W621520 USB cable $ 28 X100111 35mm replacement Uniblitz shutter $ 900(not an upgrade) X100208 8 inch guide rod for stand (each) $ 50 X100210 10 inch guide rod for stand (each) $ 55Mounting adapters for Nikon, Zeiss, Leica and Olympus microscopes areavailable. Please see the Microscope Adapters section of the catalog orcontact Sutter Instrument for pricing and further information.1Slide-in filter holders are for wheels with a shutter.2Drop-in filter holders are for wheels without a shutter.134 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

OEM WHEELS AVAILABLEFor OEM applications, the Lambda 10-3 can readily be modified to meet your specifi c requirements.By utilizing the 10-3 controller to drive customized wheels, we can provide an economical solutionto your OEM design specifi cation. To date we have made custom 2, 3, 4, 5, 10 and 12 positionwheels and have designed for 50mm, 32mm and 25mm filters. Our technical staff will be happyto discuss with you special application requirements.OPTICAL INSTRUMENTSPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM135

LAMBDA 10-2OPTICAL FILTER CHANGEROPTICAL INSTRUMENTSFEATURES LAMBDA 10-2 55msec between adjacent filters Parallel and serial interfaces Dual integral shutter drivers Linear power supply Simultaneous and independent control ofup to two wheels and two shuttersOPTIONS LAMBDA 10-2 Additional filter wheel Built-in shutter Dual wheels mounted in series Microscope mounting adapters Table-top controller box Liquid light guide Slide-in filter holders136 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The Lambda 10-2 is the ideal choice for researchers whose applications are intolerant of electricalnoise. The low-noise linear electronics of the controller assure that only DC voltages are presenton the motor windings when the wheels are not moving.The Lambda 10-2 is capable of independently operating up to two filter wheels and two Uniblitz ®shutters. The reliable, smooth operation of the Lambda 10-2 has made it highly suitable forresearch applications involving fluorescence microscopy, ratio imaging, spectrophotometry,and visual physiology.The controller electronics are designed around three embedded chips: one slave for eachwheel and a master for control and communication.OPTICAL INSTRUMENTSThe Lambda 10-2 system can hold up to ten optical filters (25mm in diameter and up to 9mm thick)which are easily loaded through an access port on the housing. Additional filters (e.g. heat and/or neutral density filters) may be inserted manually at two other locations in the optical path. Theoptical ports on both sides of the unit are internally threaded as C-mounts (32 threads per inch).When mounted in an upright position, the center of the optical port can be positioned from 11cmto 53cm above the mounting surface. Slide-in filter holders are optionally available for invertedconfi gurations. A second wheel can be added to the system with no need for additional mechanicalor electronic changes.Eight user selectable speeds are provided. Filter to adjacent filter time can be set from 55 to1200msec, depending on the number and the weight of the filters mounted in the wheel. Integralshutter drivers will open the optional shutter in 10msec. The use of a direct drive stepper-motorwith advanced microstepping technology and intelligent acceleration and deceleration algorithmsassures the fastest possible moves for a given inertial load, while minimizing vibrations.The Lambda 10-2 consists of a 10-position filter wheel, a rack mountable controller, a support baseand mounting rods, a controller/filter wheel interface cable, parallel and serial interface cables,two drop-in filter holders, spanner wrench, and manual. Microscope adapters for most Nikon,Olympus, Zeiss, and Leica scopes can be purchased separately.An optional liquid light guide output is available for applications requiring absolute vibrationisolation, and/or uniform spatial illumination. The two meter long guide mounts directly ontothe output port of the wheel. Transmission through the guide is better than 70% from 375nm to575nm. Adapters to connect the light guide to your microscope port are available as an option.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM137

SPECIFICATIONS LAMBDA 10-2OPTICAL INSTRUMENTS Filter Diameter Dimensions Weight Electrical1in25mm35mm (for Lambda 10-232)Controller:16in x 11in x 3.5in41cm x 28cm x 9cmFilter wheel:5in x 5in x 2.25in13cm x 13cm x 6cm25lbs11kg120/240 Volts50/60 Hertz power lineOTHER MODELSThe Lambda 10-232, was designed to remedy the problem of vignetting that may occur with a25mm filter format in certain microscope systems. The system features a modified Lambda10-2 controller but includes a ten position wheel that accommodates 32mm diameter filters,up to 9mm thick. The best filter to adjacent filter switching speed in this system is achieved in75 msec. A shutter for the system is optionally available. A T-mount format is used for mountingthe wheel onto your microscope. Contact Sutter Instrument for information on availableT-mount adapters.138 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES LAMBDA 10-2International prices are 5% higher. Prices subject to change without notice.SYSTEMS LB10-2 Includes one 25mm filter wheel without shutter, $ 5,400support base with mounting rods, rack mount 1 controllerunit with wheel drive cable, parallel and serial interfacecables for connection to PC, two drop-in filter holders,spanner wrench, 10 blanking discs, power cord, and manual LB10-2S Same as above—with Uniblitz ® shutter assembly $ 6,040and two slide-in filter holders LB10-232 Same as LB10-2 except with 32mm, T-mount $ 5,670threaded filter wheel (no drop-in filters) and modified10-2 controller LB10-232S Same as LB10-232 except with Uniblitz ® shutter $ 6,750(no drop-in or slide-in filters) and modified 10-2 controller LB10-250 Same as LB10-2 except with 50mm, 5 position wheel $ 6,200OPTICAL INSTRUMENTS1Tabletop controller box available on request.WHEELS25mm Filter Wheels – 10 position LB10-NW 10 position 25mm filter wheel without shutter $ 2,900 LB10-NWS 10 position 25mm filter wheel with Uniblitz ® shutter $ 3,500 LB10-NWE 10 position 25mm filter wheel set up for emission $ 2,62525mm Dual Filter Wheels LB10-WD Back to back mounting of two 25mm filter $ 5,775wheels without shutter. No drop-in filter holders LB10-WDS Back to back mounting of two 25mm filter wheels with $ 6,560one Uniblitz ® shutter and one slide-in filter holder32mm Filter Wheels – 10 position LB10-W32 10 position 32mm filter wheel without shutter $ 3,150 LB10-W32S 10 position 32mm filter wheel with Uniblitz shutter $ 4,37550mm Filter Wheel – 5 position LB10-W50 5 position 50mm filter wheel without shutter $ 3,675OEM WHEELS AVAILABLEFor OEM applications, the Lambda 10-2 can readily be modified to meet your specifi c requirements.By utilizing the 10-2 controller to drive customized wheels, we can provide an economical solutionto your OEM design specifi cation. To date we have made custom 2, 3, 4, 5, 10 and 12 positionwheels and have designed for 50mm, 32mm and 25mm filters. Our technical staff will be happyto discuss with you special application requirements.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM139

US PRICES LAMBDA 10-2International prices are 5% higher. Prices subject to change without notice.OPTICAL INSTRUMENTSCABLES AND ACCESSORIES SLIDE-IN 1 Slide-in filter holder for 25mm wheel $ 75 DROP-IN 2 Drop-in filter holder for 25mm wheel $ 75 LLG Liquid light guide (2 meters, 3mm diameter), $ 1,400C-mount, lens, and lens tube CLAM10 25 pin breakout to BNC cable $ 100 CSHUTTER Shutter breakout cable $ 100 SHUTTER 25mm replacement shutter for Uniblitz® shutter $ 500(not an upgrade) W621510 15 pin shielded cable for standard filter wheel $ 28 W621512 25 pin cable for wheels with Smart Shutter $ 28 X100111 35mm replacement Uniblitz ® shutter (not an upgrade) $ 900Mounting adapters for Nikon, Zeiss, Leica and Olympus microscopes areavailable. Please call Sutter Instrument for pricing and further information.Also, please see the Microscope Adapters section of this catalog.1Slide-in filter holders are for use with Lambda 10-2 with shutter.2Drop-in filter holders are for use with Lambda 10-2 without shutter.140 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

OPTICAL INSTRUMENTSPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM141

LAMBDA 10-BOPTICAL FILTER CHANGEROPTICAL INSTRUMENTS(Shown with LB10-NWIQ)FEATURES LAMBDA 10-B 40msec between adjacent filters(10 position wheel) 31msec between adjacent filters(4 position wheel) Serial and USB interfaces Can control a variety of 25mm, 32mm,and 50mm wheels Controls one wheel and one optionalshutter, or two Smart Shutter Can accommodate Smart Shutter and Uniblitz ® shutter Chopper drives Universal power supply142 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The Lambda 10-B is a high performance, microprocessor-controlled filter wheel for imaging applicationsrequiring a single filter wheel. The latest release in Sutter’s optical filter changer product line, theLambda 10-B, uses recent advances in motor technology to achieve switching times of 40msec betweenadjacent filters. When used with the high-speed 4 position wheels, the Lambda 10-B achieves switchingtimes of less than 31msec. It features both USB and serial port interfaces as well as keypad control.The Lambda 10-B is capable of controlling both the Smart Shutter as well as the Uniblitz ® shutter. TheLambda 10-B is a low-cost alternative and ideal for imaging applications requiring a single filter wheel.The Lambda 10-B can operate with switching times as brief as 40msec with our standard 25mm filterwheel as well as our 32mm wheel. The user can select from seven speeds, allowing the speed to beadjusted in accordance with the load of the wheel. Except for the 4 position compact wheel, our filterwheels employ a direct drive system: a feature that prevents belt slippage or backlash. An optionalshutter installation is also available.OPTICAL INSTRUMENTSFilter selection can be made directly from the keypad or from a computer via the serial or USB port.The Lambda 10-B controller determines the shortest route to the selected filter and an acceleration/deceleration algorithm minimizes vibrations during the movement of the wheel. The current filter positionis displayed on the front panel. Internal sensors monitor the position of the filter wheel to insure that thecorrect filter is in place. The drive uses switching mode current regulation, which is more economicalthan the linear supply of the Lambda 10-2. Although this is not a problem with most imaging applications,the Lambda 10-2 may be more appropriate for systems where electrophysiology is performed.Both serial and USB input ports are provided to allow complete and easy control from a remote computer.The serial port accepts RS232 level signals through a DB-9 connector. USB input is made through astandard connector and can be directly connected to a USB port. The Lambda 10-B’s USB port interfacesupports multiple USB devices simultaneously, allowing a user to run in tandem as many units as USBports. The universal power supply will automatically switch to accommodate local line voltage.OPTIONS:The basic system for the Lambda 10-B includes our popular 10 position 25mm filter wheel. A significantadvantage of the Lambda 10-B controller is that it can accommodate a variety of Sutter filter wheelsto suit your particular requirements. The controller will automatically detect and determine the modelof wheel installed and adjust for the number of positions and filter size.Our 32mm wheel was designed to remedy the problem of vignetting that may occur with a 25mmfilter format in certain microscope systems, and can achieve the same 40msec switching times as our25mm wheel.For applications requiring a larger aperture opening, there is a 5 position 50mm filter wheel. If additionalspeed is required, we offer a high-speed, 4 position 25mm wheel which is capable of achieving 31msecswitching times between adjacent filters. An optional liquid light guide is available for applicationsrequiring absolute vibration isolation, and/or spatial uniformity independent of wavelength. TheLambda 10-B is also capable of controlling either the SmartShutter or the Uniblitz shutter.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM143

SPECIFICATIONS LAMBDA 10-BOPTICAL INSTRUMENTS Control BoxDimensions Weight Electrical8.2in x 5.9in x 5.1in14.9cm x 20.8cm x 13cm2.6lbs1.1kg120/240 Volts50/60 Hertz power line75 Watts maxRoHS Compliant144 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES LAMBDA 10-BInternational prices are 5% higher. Prices subject to change without notice.CONTROLLER LB10-B/IQ 1 Lambda 10-B control unit, serial and USB cables, $ 1,600power cord and manualWHEELS25mm Filter Wheels - 10 position LB10-NW 10 position 25mm filter wheel without shutter $ 2,900 LB10-NWS 10 position 25mm filter wheel with SmartShutter $ 3,500 LB10-NWS 10 position 25mm filter wheel with Uniblitz ® shutter $ 3,500 LB10-NWE 10 position 25mm filter wheel set up for emission $ 2,62525mm Filter Wheels - 4 position LB10-WHS4 4 position 25mm filter wheel without shutter $ 2,950 LB10-WHS4IQ 4 position 25mm filter wheel with SmartShutter $ 3,725 LB10-WHS4E 4 position 25mm filter wheel set up for emission $ 2,95032mm Filter Wheels - 10 position LB10-W32 10 position 32mm filter wheel without shutter $ 3,150 LB10-W32IQ 10 position 32mm filter wheel with SmartShutter $ 4,725 LB10-W32S 10 position 32mm filter wheel with Uniblitz shutter $ 4,37550mm Filter Wheel - 5 position LB10-W50 5 position 50mm filter wheel without shutter $ 3,675Specialty Wheels LB4-W Belt drive 4 position 25mm filter wheel without shutter $ 2,950OPTICAL INSTRUMENTSSMARTSHUTTER IQ12-SA 12.5mm SmartShutter with stand-alone housing $ 995 IQ25-SA 2 25mm SmartShutter with stand-alone housing $ 1,050 IQ25-W 3 25mm SmartShutter with housing to fit filter wheel $ 1,050 IQ25-LS 25mm SmartShutter with housing to fi t Lambda LS $ 1,100 IQ25-DG 25mm SmartShutter to fi t in Lambda DG-4/DG-5 $ 1,450 IQ35-W 35mm SmartShutter with housing to fit filter wheel $ 1,525 IQ35-SA 35mm SmartShutter with stand-alone housing $ 1,5251CIQ-2 cable provided when ordered with stand-alone shutter.2Where vignetting may be an issue, we recommend the 35mm shutter.3For upgrading a 25mm filter wheel with existing Uniblitz shutter to SmartShutter.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM145

US PRICES LAMBDA 10-BInternational prices are 5% higher. Prices subject to change without notice.OPTICAL INSTRUMENTSACCESSORIES CIQ-2 One 25 pin connector to two 9 pin connectors $ 100(Connects up to 2 stand-alone shutters toLB10-B/IQ controller) FSWITCH Foot switch with BNC connector – $ 100Changes state of shutter with foot press as long asfoot press is maintained FSTOGGLE Foot switch with BNC connector – $ 100Alternates open/close with each foot press W621510 15 pin shielded cable for standard filter wheel $ 28 W621512 25 pin cable for wheels with SmartShutter $ 28 W621520 USB cable $ 28 SLIDE-IN 1 Slide-in filter holder for 25mm wheel $ 75 DROP-IN 2 Drop-in filter holder for 25mm wheel $ 75 DROP-IN/32 2 32mm drop-in filter holder for LB10-W32IQ $ 75 LLG Liquid light guide (2 meters, 3mm diameter), $ 1,400C-mount, lens, and lens tube SHUTTER 25mm replacement shutter for Uniblitz ® $ 500shutter (not an upgrade) X100111 35mm replacement Uniblitz shutter (not an upgrade) $ 900SHUTTER MOUNTING POSTStainless steel 1/2 in diameter. One end is 1/4in-20 (M6) tapped hole. Other endis #8-32 (M4) removable threaded stud. Can be used with 25mm SmartShutter. O620120 1in/25mm long post $ 15 O620122 1.5in/38mm long post $ 15 O620123 2in/50mm long post $ 15POST HOLDER O620125 Holder and spring loaded thumbscrew for mounting post $ 15POST BASE PLATE O620140 2in x 3in x 3/8in base plate. Suitable for Imperial or $ 15Metric tables, posts, and post holdersPOST COLLAR O620150 Slip-on collar, 1in OD x 0.5in ID $ 15Mounting adapters for Nikon, Zeiss, Leica and Olympus microscopes areavailable. Please call Sutter Instrument for pricing and further information.Also, please see the Microscope Adapters section of this catalog.1Slide-in filter holders are for use with Lambda 10-2 with shutter.2Drop-in filter holders are for use with Lambda 10-2 without shutter.146 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

FILTER WHEEL CONTROLLER COMPARISON CHARTFeatures Lambda 10-3 Lambda 10-2 Lambda 10-BMaximum # of Wheels 3 2 1Maximum # of Shutters 3 1 2 2 1Shutter TypeSmart Shutter OPTICAL INSTRUMENTSUniblitz ® Filter Diameter (mm)12/25/32/50 2 25/32/50 3 Minimum Switching Time 4 (ms)40ms - 25mm wheel 50ms - 32mm wheel 31ms - 4 position 25mm 55ms - 25 or 32mm wheel33ms - high speed wheelPower Supply Chopper Linear 5 ChopperComputer InterfaceSerial Parallel USB TTL In/Out Yes No YesPrice 6 $5,420 $5,400 $4,5001. The Lambda 10-3 can be used to drive up to 3 filter wheels and 2 shutters, or 3 shutters and 2 filter wheels.The Lambda 10-B, two shutters or one wheel and one shutter.2. The controller automatically detects the installed hardware.3. The controller has to be modified for the larger filter versions of the wheels.4. Minimum switching time between adjacent filters depends on the filter load. The given values are for a load of 2 filters.5. Recommended for applications requiring low electrical noise (i.e. electrophysiology).6. For one controller with one non-shuttered standard 10 position 25mm filter wheel. U.S. prices.International prices are 5% higherPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM147

LAMBDA SCSMARTSHUTTER CONTROLLEROPTICAL INSTRUMENTS(Shown with IQ35-SA and stand)FEATURESSMARTSHUTTER Robust design Life tested to 100 million cycles Modular repairable design Opening time 8msec from trigger(for 25mm and 35mm versions) Continuous operation frequencies ashigh as 40Hz Stand-alone or use with Sutter filter wheel Mountable in Lambda LS, Lambda DG-4/DG-5,and Lambda XLFEATURESLAMBDA SC Microprocessor based controller Serial, USB and TTL interfaces User can produce a variable aperture byselecting the degree of shutter opening “Soft” action mode provides minimum vibration TTL activation of the shutter can be set tohigh, low, or Toggle on rising/falling edge Programmable delay and exposure intervalsof up to 5 hours with millisecond resolution Commands can run continuously or loop aspecified number of times Programmable delayed sync out Universal power supply148 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The Lambda SC is a full-featured micro-processor controlled shutter driver for the SmartShutter .It supports the same shutter command set used for our other Lambda controllers and offersenhanced performance through additional modes of operation. The additional operating modesallow programmable control over the exposure time, the delay between exposures, the numberof exposures, the mode of triggering and the mode of shutter operation. The Lambda SC utilityprogram allows computer control and confi guration of multiple SC controllers through serial orUSB ports.In the Lambda 10 family of filter wheel controllers, shutter timing is controlled directly by thetime of occurrence of commands or TTL input. In most cases, the system software used withthese controllers has provisions for controlling the timing of the shutter. The Lambda SC shuttercontroller may be used in applications that would benefi t from enhanced control options built intothe controller. A convenient toggle switch on the front panel has three positions: OPEN (shutterwill open regardless of other inputs), CLOSED (shutter will close regardless of other inputs) andAUTO. In the AUTO position, the controller can be operated remotely from a computer throughthe USB port, or opened and closed using a logic level input.OPTICAL INSTRUMENTSIn the free running mode, the shutter will open and close repeatedly. The time between closingand reopening can either be directly timed, or set as an interval for the entire cycle. Delay andexposure intervals can be set by the user for up to 5 hours with millisecond resolution. This modecan be set to continuously run or loop for a specifi c number of cycles. The mode is initiated frompower up, or a trigger pulse, and can be interrupted by using a stop command.Because the SmartShutter incorporates a microprocessor controlled motor/wiper design, thetrajectory of each move can be controlled and the motion of the blade optimized for speed or forsmoothness. When speed is the most important condition, the user can select the fast mode.If the modest amount of vibration in the fast mode is objectionable and speed is not important,the “soft” mode can be selected, moving the blade slower through the overall travel.The neutral density mode results in a partial opening of the SmartShutter. Any one of the144 steps from a fully closed to fully open can be selected. Using this variable apertureopening along with a liquid light guide acts to spatially homogenize the light to produce auniform spatial illumination that’s independent of the geometry of the input illumination.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM149

SPECIFICATIONSLAMBDA SCOPTICAL INSTRUMENTS Dimensions Weight Electrical5.5in x 8.5in x 2.325in14cm x 21.6cm x 6cm1.7lbs0.77kg120/240 Volts50/60 Hertz power line75 Watts maxRoHS CompliantUS PRICES LAMBDA SCInternational prices are 5% higher. Prices subject to change without notice.CONTROLLER LB-SC Includes one Lambda SC control unit, serial and $ 790USB cable, power cord and manualSMARTSHUTTER IQ12-SA 12.5mm SmartShutter with stand-alone housing $ 995 IQ25-SA 1 25mm SmartShutter with stand-alone housing $ 1,050 IQ25-LS 25mm SmartShutter with housing to fi t Lambda LS $ 1,100 IQ25-DG 25mm SmartShutter to fi t in Lambda DG-4/DG-5 $ 1,450 IQ35-W 35mm SmartShutter with housing to fit filter wheel $ 1,525 IQ35-SA 35mm SmartShutter with stand-alone housing $ 1,5251Where vignetting may be an issue, we recommend the 35mm shutter.150 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES LAMBDA SCInternational prices are 5% higher. Prices subject to change without notice.CABLES AND ACCESSORIES W621520 USB cable $ 28 W620005 9 pin male/female SmartShutter (no wheel) $ 28 FSWITCH Foot switch with BNC connector – $ 100Changes state of shutter with foot press as long asfoot press is maintained FSTOGGLE Foot switch with BNC connector – $ 100Alternates open/close with each foot pressOPTICAL INSTRUMENTSSHUTTER MOUNT POSTSStainless steel 1/2 in diameter. One end is 1/4in-20 (M6) tapped hole. Other endis #8-32 (M4) removable threaded stud. Can be used with 25mm SmartShutter. O620120 1in/25mm long post $ 15 O620122 1.5in/38mm long post $ 15 O620123 2in/50mm long post $ 15POST HOLDER O620125 Holder and spring loaded thumbscrew for mounting post $ 15POST BASE PLATE O620140 2in x 3in x 3/8in base plate. Suitable for Imperial or $ 15Metric tables, posts, and post holdersPOST COLLAR O620150 Slip-on collar, 1in OD x 0.5in ID $ 15Mounting adapters for Nikon, Zeiss, Leica and Olympus microscopes areavailable. Please call Sutter Instrument for pricing and further information.Also, please see the Microscope Adapters section of this catalog.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM151

SMARTSHUTTER STEPPER-MOTOR DRIVEN SHUTTEROPTICAL INSTRUMENTS(Shown with LB10-B/IQ controller)FEATURESSMARTSHUTTER Robust design Life tested to 100 million cycles Modular repairable design Opening time 8msec from trigger(for 25mm and 35mm versions) Continuous operation frequencies ashigh as 40Hz “Soft” action mode provides minimumvibration Selection partial opening function forneutral density Serial, USB and TTL interfaces Manual input via keypad (on LB10-B/IQ) Universal power supply Stand-alone or use with Sutter filter wheel Patent No. 7,253,575 Mountable in Lambda LS, Lambda DG-4/DG-5,and Lambda XL Microprocessor based controllerRoHS Compliant152 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The SmartShutter is designed to complement our growing line of optical products and sets a newstandard for shutter performance and reliability. In the traditional shutter design there are two or more“leaves” that rub against each other. Given time, the blades will wear down, bind, and the shutter willfail to open. The SmartShutter is designed with only one moving part, which virtually eliminates theeffects of wear and markedly improves performance. In the traditional shutter design there is an additionalissue of the leaves binding in extreme temperature conditions. To solve this problem our shutter bladehas the ability to perform well under very high temperature conditions and extending the life of the shutter.The SmartShutter incorporates a new high-performance motor drive and precision stepper-motor toprovide added control and durability. Traditional shutters use a solenoid actuator requiring a high initialopening voltage for rapid opening of the shutter. This can overheat or burn out the coil of the shutter ifthe shutter is opened too frequently. With our microprocessor controlled motor/wiper design, we cancontrol the trajectory of each move and optimize the motion of the blade for speed or for smoothness. TheSmartShutter, in either the 25mm or 35mm version, operates with open/close times of 8msec from thecommand (3msec from the start of motion). Since our robust design does not rely on over-driving thewindings, we can achieve continuous operation at certain frequencies up to 40 Hz for the 25mm version,and 20 Hz for the 35mm model. While the 25mm SmartShutter can run at frequencies up to 40 Hz,some repetition rates may excite undesirable resonances that interfere with proper operation.A small adjustment in frequency will normally correct this.OPTICAL INSTRUMENTSBecause the shutter blade is stopped by the action of the motor rather than mechanical stops,SmartShutter units tested for over 100 million cycles show no sign of failure. The standardSmartShutter blade is made of untreated aluminum. Non-reflective coatings are available uponrequest and are not recommended for use with arc lamps.The microprocessor-based controller provides exceptional versatility and is adaptable to variousmodes of operation and function. The SmartShutter can be programmed for a variety of movementprofiles or to produce a variable aperture by determining the degree of shutter opening. When usedin our Lambda LS xenon light source and coupled with a liquid light guide, the shutter acts as aprogrammable neutral density filter. In addition, while the impulse of our shutter is minimal,a “soft” action mode can be selected to decrease vibration.The LB10-B/IQ controller for the SmartShutter is capable of driving up to two shutters. To support thisfunction a TTL input is supplied for remote triggering of the shutter, as well as a TTL output to supportremote triggering of external devices such as a camera. The controller can also be operated locally(manually) from the controller keypad or remotely from a computer through either the USB or serial port.A dedicated single shutter controller, the Lambda SC is also available and provides a USB port, serial,TTL in and TTL sync out. The Lambda SC allows programmable control over the exposure time, andthe delay between trigger and shutter opening.Originally designed to be integrated into our 25mm and 32mm filter wheels, housings are also availablefor stand-alone units and for use in our Lambda LS, Lambda XL, and Lambda DG-4/DG-5 opticalproducts. The standard SmartShutter blade is made of untreated aluminum. Non-reflective coatingsare available upon request and are not recommended for use with arc lamps. The modularity of theSmartShutter assures that repairs, should they be necessary, are simple and economical.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM153

US PRICES SMARTSHUTTER International prices are 5% higher. Prices subject to change without notice.OPTICAL INSTRUMENTSCONTROLLER LB10-B/IQ Lambda 10-B control unit, serial and USB cables, $ 1,600power cord and manual LB-SC Includes one Lambda SC control unit, serial and $ 790USB cable, power cord and manualSMARTSHUTTER IQ12-SA 12.5mm SmartShutter with stand-alone housing $ 995 IQ25-SA 1 25mm SmartShutter with stand-alone housing $ 1,050 IQ25-W 2 25mm SmartShutter with housing to fit filter wheel $ 1,050 IQ25-LS 25mm SmartShutter with housing to fi t Lambda LS $ 1,100 IQ25-DG 25mm SmartShutter to fi t in Lambda DG-4/DG-5 $ 1,450 IQ35-W 35mm SmartShutter with housing to fit filter wheel $ 1,525 IQ35-SA 35mm SmartShutter with stand-alone housing $ 1,525 IQ50-SA 50mm SmartShutter with stand-alone housing $ 1,5251Where vignetting may be an issue, we recommend the 35mm shutter.2For upgrading a 25mm filter wheel with existing Uniblitz ® shutter to SmartShutter.(Shown: IQ25-SA)154 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES SMARTSHUTTER International prices are 5% higher. Prices subject to change without notice.CABLES AND ACCESSORIES CIQ-2 One 25 pin connector to two 9 pin connectors $ 100(Connects up to 2 stand-alone shutters toLB10-B/IQ controller) W621520 USB cable $ 28 W620005 9 pin male/female SmartShutter (no wheel) $ 28 FSWITCH Foot switch with BNC connector – $ 100Changes state of shutter with foot press as long asfoot press is maintained FSTOGGLE Foot switch with BNC connector – $ 100Alternates open/close with each foot pressOPTICAL INSTRUMENTSMOUNTING POSTSStainless steel 1/2 in diameter. One end is 1/4in-20 (M6) tapped hole. Other end is#8-32 (M4) removable threaded stud. Can be used with 25mm SmartShutter. O620120 1in/25mm long post $ 15 O620122 1.5in/38mm long post $ 15 O620123 2in/50mm long post $ 15POST HOLDER O620125 Holder and spring loaded thumbscrew for mounting post $ 15POST BASE PLATE O620140 2in x 3in x 3/8in base plate. Suitable for Imperial or $ 15Metric tables, posts, and post holdersPOST COLLAR O620150 Slip-on collar, 1in OD x 0.5in ID $ 15Mounting adapters for Nikon, Zeiss, Leica and Olympus microscopes areavailable. Please call Sutter Instrument for pricing and further information.Also, please see the Microscope Adapters section of this catalog.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM155

MICROSCOPE ADAPTERSOPTICAL INSTRUMENTSORDERING INFORMATIONIt is advisable to contact Sutter Instrument to discuss your adapter needs prior to purchasing.All prices are U.S. prices. Pricing in other countries may vary.LAMBDA FILTERWHEELSExcitation adapters (suffi x EC) include all components necessary for mounting our filter wheelsbetween the microscope lamp housing and excitation port. Please select adapters with a prefi xof 10 for wheels with a 25mm aperture, and a prefi x of 32 for wheels with a 32mm aperture.The lamp housing adapter (suffi x LH) is only required when the LLG (Liquid Light Guide) is orderedand used with a filter wheel and microscope lamp housing when not attached to the scope.The lamp house adapter is not needed when the filterwheel is used with the Lambda LS orLambda XL light source.LAMBDA DG-4/DG-5 PLUSThe Lambda DG-4/DG-5 PLUS will only require a light guide adapter (prefi x LG).LAMBDA LSIt is recommended that the Lambda LS be used with the optional LLG (Liquid Light Guide)and a light guide adapter (prefi x LG).LAMBDA XLTo couple a Lambda XL to a microscope, you will need either the LLG/XL, LLG/5XL or the LLG/380XL(Liquid Light Guide) and a light guide adapter (prefi x LG). Some microscope adapters will only havethe Large Format (suffi x LF) versions available as that is the only suitable solution.LARGE FORMAT LIGHT GUIDE ADAPTERSThe large format light guide adapter (suffi x LF) is necessary when a demagnification lens is presentin the emission path to eliminate vignetting in the camera. This adapter is also recommended forthose customers wishing to eliminate vignetting in the eyepiece.156 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

SMARTSHUTTERThe SmartShutter can be mounted on the microscope excitation and transmitted light ports usingour standard adapters. For easy reference:IQ25 Excitation – use adapters that begin with 10IQ35 Excitation – use adapters that begin with 32IQ25 & IQ35 Transmitted light – use adapters ending with TLMICROSCOPE ADAPTERSOLYMPUSBX-50 / BX-60 (Y50)10-Y50-EC EXCITATION $ 39032-Y50-EC EXCITATION – 32mm $ 39010-Y50-EM EMISSION $ 55010-Y50-LH LAMP HOUSING $ 195LG-Y50 LIGHT GUIDE $ 550LG-Y50-LF LIGHT GUIDE – Large Format $ 99025-Y50-TL TRANSMITTED (25mm) $ 39035-Y50-TL TRANSMITTED (35mm) $ 390OPTICAL INSTRUMENTSBX-51 / BX-61 (Y51)10-Y51-EC EXCITATION $ 39032-Y51-EC EXCITATION – 32mm $ 39010-Y51-EM EMISSION $ 55010-Y51-LH LAMP HOUSING $ 195LG-Y51 LIGHT GUIDE $ 550LG-Y51-LF LIGHT GUIDE – Large Format $ 99025-Y51-TL TRANSMITTED (25mm) $ 39035-Y51-TL TRANSMITTED (35mm) $ 390IX-70 / IX-50 (Y70)10-Y70-EC EXCITATION $ 39010-Y70-EM EMISSION (side port of scope $ 550needs modifi cation at Sut ter)10-Y70-LH LAMP HOUSING $ 195LG-Y70 1 LIGHT GUIDE $ 55025-Y70-TL TRANSMITTED (25mm) $ 39035-Y70-TL TRANSMITTED (35mm) $ 390IX-51 / IX71 / IX-81 (Y71)10-Y71-EC EXCITATION $ 39032-Y71-EC EXCITATION – 32mm $ 39010-Y71-EM EMISSION $ 55010-Y71-LH LAMP HOUSING $ 195LG-Y71 1 LIGHT GUIDE $ 550LG-Y71-LF LIGHT GUIDE – Large Format $ 99025-Y71-TL TRANSMITTED (25mm) $ 39035-Y71-TL TRANSMITTED (35mm) $ 3901Replaces the epi-illuminatorPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM157

MICROSCOPE ADAPTERSOPTICAL INSTRUMENTSNIKONTMD (N10)TS100 (N15)DIAPHOT 200/300 (N20)OPTIPHOT (N30)Please contact Sutter to discuss your needs.TE200/300 (N25)10-N25-EC EXCITATION $ 33032-N25-EC EXCITATION – 32mm $ 33010-N25-EM EMISSION (needs 1x relay lens) $ 39035-N25-TL* TRANSMITTED – 35mm $ 33010-N25-LH LAMP HOUSING $ 17010-N25-QB BOTTOM (QUANTUM) $ 390LG-N25 1 LIGHT GUIDE $ 770TE2000 (N27)10-N27-EC EXCITATION $ 33032-N27-EC EXCITATION – 32mm $ 33010-N27-EM EMISSION $ 39035-N27-TL TRANSMITTED – 35mm $ 33010-N27-LH LAMP HOUSING $ 170LG-N27 1 LIGHT GUIDE $ 770LG-N27-LF LIGHT GUIDE – Large Format $ 99010-N27-SU 2 STAGE UP $ 1,390Ti (N29)10-N29-EC EXCITATION $ 33032-N29-EC EXCITATION – 32mm $ 33010-N29-EM EMISSION $ 39035-N29-TL TRANSMITTED – 35mm $ 33010-N29-LH LAMP HOUSING $ 170LG-N29 1 LIGHT GUIDE $ 770LG-N29-LF LIGHT GUIDE – Large Format $ 99010-N29-SU 3 STAGE UP $ 1,78010-N29-SB 4 STAGE UP – Bracket only $ 625MA-N29 5 MOSAIC $ 2,4501Replaces the epi-illuminator2Places wheel in infinity path below the dichroic cassette3For use in conjunction with standard Nikon 70mm stage up kit (MED532000)4For use with infinity path emission wheel applications only, not applicable for anyother applications5For use with Mosaic and TIRF attachment*Please contact Sutter to discuss solutions and early model Nikon scope adapters.158 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

MICROSCOPE ADAPTERSNIKON (CONTINUED)E400 & E600 (N40)10-N40-EC 1 EXCITATION $ 55032-N40-EC 1 EXCITATION – 32mm $ 33010-N40-EM EMISSION $ 39010-N40-LH LAMP HOUSING $ 275LG-N40 2 LIGHT GUIDE $ 550LG-N40-R 3 LIGHT GUIDE $ 525FN1 (N65)10-N65-EC EXCITATION $ 33032-N65-EC EXCITATION – 32mm $ 33010-N65-EM EMISSION $ 39010-N65-LH LAMP HOUSING $ 170LG-N65-LF LIGHT GUIDE – Large Format $ 990OPTICAL INSTRUMENTSE800 & E1000 (N80)10-N80-EC EXCITATION $ 33032-N80-EC EXCITATION – 32mm $ 33010-N80-EM EMISSION $ 39010-N80-LH LAMP HOUSING $ 170LG-N80 LIGHT GUIDE $ 55050i / 80i / 90i (N85)10-N85-EC EXCITATION $ 44032-N85-EC EXCITATION – 32mm $ 44010-N85-EM 4 EMISSION $ 39010-N85-IN 5 EMISSION – INFINITY $ 750LG-N85 LIGHT GUIDE $ 660LG-N85-LF LIGHT GUIDE – Large Format $ 99035-N85-TL TRANSMITTED – 35mm $ 3901We’ll need to know which epi-illuminator you have2For use with sliding dichroic illuminator3For use with rotating dichroic illuminator4Standard trinocular head. Phone Sutter if you have the digital imaging head.5Puts filter wheel in emission infinity path. May cause vignetting.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM159

MICROSCOPE ADAPTERSOPTICAL INSTRUMENTSZEISSAXIOSKOP 2 & 2 FS (Z25)10-Z25-EC EXCITATION $ 23010-Z25-EM 1 EMISSION $ 44035-Z25-TL TRANSMITTED – 35mm $ 33010-Z25-LH LAMP HOUSING $ 120LG-Z25 LIGHT GUIDE $ 550AXIOVERT 35 and 100 series (Z30)10-Z30-EC EXCITATION $ 23010-Z30-LH LAMP HOUSING $ 120LG-Z30 2 LIGHT GUIDE $ 55010-Z30-KP KELLER PORT $ 39010-Z30-KP-M KELLER PORT (100M SCOPE) $ 39010-Z30-SP SIDE PORT $ 44010-Z30-TH TRINOCULAR HEAD $ 44010-Z30-TH-M TRINOCULAR HEAD (135M SCOPE) $ 420AXIOVERT 200 (Z35)10-Z35-EC EXCITATION $ 23010-Z35-LH LAMP HOUSING $ 12032-Z35-EC EXCITATION – 32mm $ 33010-Z35-EM EMISSION (side port on left) $ 66010-Z35-EM-1M 200M – EMISSION (side port on left) $ 66035-Z35-TL TRANSMITTED – 35mm $ 33010-Z35-KP KELLER PORT $ 550LG-Z35 LIGHT GUIDE $ 550LG-Z35-LF LIGHT GUIDE – Large Format $ 9901Please specify standard or ergonomic trinocular head2Replaces the epi-illuminator160 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

MICROSCOPE ADAPTERSZEISS (CONTINUED)AXIO OBSERVER (Z40)10-Z40-EC EXCITATION $ 23032-Z40-EC EXCITATION – 32mm $ 33010-Z40-LH LAMP HOUSING $ 12010-X10-EM EMISSION $ 3,200(includes the Photometrics XT2)LG-Z40 1 LIGHT GUIDE $ 550LG-Z40-LF LIGHT GUIDE – Large Format $ 99025-Z40-TL TRANSMITTED (25mm) $ 37035-Z40-TL TRANSMITTED (35mm) $ 370AXIO IMAGER (Z45)AXIO EXAMINER10-Z45-EC EXCITATION $ 23032-Z45-EC EXCITATION – 32mm $ 33010-Z45-LH LAMP HOUSING $ 12010-X10-EM EMISSION $ 3,200(includes the Photometrics XT2)LG-Z45 LIGHT GUIDE $ 550LG-Z45-LF LIGHT GUIDE – Large Format $ 990TRANSMITTED (25mm)Phone SutterTRANSMITTED (35mm)Phone SutterOPTICAL INSTRUMENTSAXIOPLAN 2 (Z50)Please contact Sutter to discuss your needs1Replaces the epi-illuminatorPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM161

MICROSCOPE ADAPTERSOPTICAL INSTRUMENTSLEICADMR (L10)10-L10-EC EXCITATION $ 33032-L10-EC EXCITATION – 32 mm $ 33010-L10-EM EMISSION $ 55010-L10-LH LAMP HOUSING $ 170LG-L10 LIGHT GUIDE $ 550DMIRB & DMIRE2 (L20)10-L20-EC EXCITATION $ 33032-L20-EC EXCITATION – 32mm $ 33010-L20-EM 1 EMISSION $ 55010-L20-LH LAMP HOUSING $ 170LG-L20 1 LIGHT GUIDE $ 660LG-L20-LF LIGHT GUIDE – Large Format $ 990DM 4000/5000/6000 (L30)10-L30-EC EXCITATION $ 33032-L30-EC EXCITATION – 32mm $ 33010-L30-EM EMISSION $ 550LG-L30 LIGHT GUIDE $ 770LG-L30-LF LIGHT GUIDE – Large Format $ 990DMI 4000/5000/6000 (L40)10-L40-EC EXCITATION $ 33032-L40-EC EXCITATION – 32mm $ 33010-L40-EM EMISSION $ 550LG-L40 2 LIGHT GUIDE $ 770LG-L40-LF LIGHT GUIDE – Large Format $ 990OTHERYOKOGAWA CSU-X1(J15)ADAPTERS 10-J15-EM 3 EMISSION $ 650CARV (J20)CARV IIPlease contact Sutter to discuss your needs.1Please specify standard or ergonomic trinocular head2Replaces the epi-illuminator3Fits both emission ports162 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

OPTICAL INSTRUMENTSPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM163

MICROINJECTIONXenoWorks ® is a modular microinjection systemdesigned to meet a wide variety of applicationneeds. Our experience with precision motorcontrol has culminated in this state-of-the-artsystem.The XenoWorks Micromanipulator uses aclassic inverted joystick design for excellentergonomics, intuitively linking the user withthe micropipette. Combined with our smooth,responsive stepper-motor drives, the XenoWorksManipulator provides exceptional mechanicalstability, sensitivity, and range of movement.The XenoWorks Digital Microinjector wasdesigned for precision manipulation of cellsvia an easy-to-use interface. The DigitalMicroinjector employs two pressure ports,one for holding suspended cells, and a secondchannel is available for both high-pressureinjection and gentle positive/ negative pressurefor transfer of embryonic stem cells. Bothinjection duration and pressure are easilyselectable using rotary knobs on the remoteinterface. The built-in compressor removes theneed for an external pressure supply.

The XenoWorks family also includes theAnalog Microinjector for simple cell holdingand transfer applications, which incorporatesthe same smooth response and ergonomicdesign of our other XenoWorks products.APPLICATIONS- Pronuclear microinjection of DNA intomouse zygote- Embryonic stem cell transfer into blastocysts- Somatic cell nuclear transfer (ES cell)- Intracytoplasmic sperm injection (ICSI)- Piezo-assisted sperm-mediated transgenesis- Nuclear transfer (NT)- Adherent cell microinjection- Drosophila injection- C. Elegans injection- Zebrafish injection- Contact Sutter for suitability of XenoWorksfor applications not listed here

XENOWORKS ®MICROMANIPULATORMICROINJECTIONFEATURESXENOWORKS ® MICROMANIPULATOR Height and tension adjustable joystick foroptimal ergonomics Exceptionally smooth and responsivemicropipette movement Touch declutch mechanism 6 speeds from coarse to ultra-fine “Home” position for fast micropipetteexchange User-definable memory positions, including“Work” and Z-axis lower limit Y-axis lock-out for pure X-axis travel 25mm of travel in all three axe166 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The XenoWorks ® Micromanipulator has been designed around our hugely-successful MP-285electrophysiology micromanipulator, with the addition of a smooth-moving adjustable invertedjoystick. Because the motor drive is based on an electrophysiology design, the XenoWorksMicromanipulator is extremely stable and resistant to ambient vibration. This stability also makesthis manipulator an ideal platform for use in conjunction with the PrimeTech PMM-150FU PiezoImpact Drive (see page 166), for applications such as mouse nuclear transfer and intracytoplasmicsperm injection.MICROINJECTIONThe joystick offers an unprecedented level of user comfort during operation. The use of an invertedjoystick in conjunction with an almost zero-profile base allows the operator to rest their handsand forearms on the bench surface, providing ease of use and added ergonomics. The functionkeys and declutch mechanism can be located and activated by touch, removing the need to lookaway from the microscope, which will save time. Another time saving feature is the “Setup”function. Activating the setup function places the micromanipulator in the optimum position priorto beginning work (halfway on the Y-axis, three-quarters down on the Z-axis and three-quartersinwards on the X-axis). Once the manipulator is in the default setup position, the micropipette tipwill be positioned within the microscope’s optical path, reducing the time required find the pipettetip through the microscope. The setup function also ensures an adequate travel range in all axes ofmotion prior to starting work.The manipulator uses a pre-programmed “Home” position for fast micropipette exchange, andthe ability to determine a “Work” position so the pipette can be repositioned with the press of abutton. A Y-axis lockout function allows X-only axial movement for maximum control duringinjections, and a Z-limit memory position is used to prevent the pipette from colliding with thebottom of the dish.The XenoWorks joystick is designed to allow the user to immediately develop an intuitive feelthat makes it easy to precisely control position. Movement of the joystick is directly converted toa proportional movement of the manipulator. Speed control, center of travel, and programmablepositions are right at your fingertips. One unique feature of the joystick is the declutch mechanismthat allows for rapid repositioning of the joystick without moving the pipette. Combining thisadvanced joystick with the proven Sutter MP-285 manipulator technology creates an ideal systemfor intensive microinjection.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM167

SPECIFICATIONSXENOWORKS ® MICROMANIPULATORMICROINJECTION Travel Maximum Resolution Maximum Speed25mm on all three axes62.5nm/microstep5.5mm/sec Range X & Y Axis Z Axis( (1 joystick swing) (1 rotation) Joystick Dimensions ElectricalC 12,700µm 3,200µm1 3,300µm 800µm2 880µm 400µm3 400µm 200µm4 200µm 100µm5 100µm 50µm• Inverted joystick with touch declutch• Height and resistance adjustable• Independent axis-polarity switches• 6 speed settings• ‘Setup’ function• 1 user-defined set point (‘Work’),• 1 user-defined axis limit (‘Z-fl oor’),• 2 preset points (‘Home’ and ‘Setup’)• Y-axis lockoutMotor drive:112mm x 185mm x 145mmJoystick:265mm x 240mm x 226mmControl:407mm x 280mm x 101mm120/240 Volts50/60 Hertz power line168 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICESXENOWORKS ® MICROMANIPULATORInternational prices are 5% higher. Prices subject to change without notice. BRML XenoWorks ® Micromanipulator (Left) $ 12,870Includes: 3 axis motor drive (left-hand confi guration),motor drive base plate, controller, joystick user-interface,connecting cables, and manual BRMR XenoWorks Micromanipulator (Right) $ 12,870Includes: 3 axis motor drive (right-hand confi guration),motor drive base plate, controller, joystick user-interface,connecting cables, and manualMICROINJECTIONXENOWORKS MICROSCOPE ADAPTERS BR-L20 Leica DMIRB/E/IRE2 adapter $ 475 BR-L30 Leica DMIL adapter $ 475 BR-L40 Leica DMI 4/5/6000 adapter (double sided) $ 525 BR-N10 Nikon TMD adapter $ 475 BR-N25 Nikon TE200/300 adapter $ 475 BR-N27 Nikon TE2000 adapter $ 475 BR-N29 Nikon Ti adapter $ 475 BR-N29-M Nikon Ti adapter for scopes with motorized stage $ 475 BR-Y50 Olympus IX50/70 adapter $ 475 BR-Y51 Olympus IX51/71/81 adapter $ 475 BR-Y51-FL Olympus IX51/71/81 adapter with ZDC autofocus, left $ 550 BR-Y51-FR Olympus IX51/71/81 adapter with ZDC autofocus, right $ 550 BR-Z30 Zeiss Axiovert 100/135 adapter $ 475 BR-Z35 Zeiss Axiovert 200 adapter $ 475 BR-Z40 Zeiss Axio Observer adapter $ 475 BR-CUST Custom microscope adapter $ 975ACCESSORIES BR-AW Rod holding clamp $ 260PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM169

XENOWORKS ®DIGITAL MICROINJECTORMICROINJECTIONFEATURESXENOWORKS ® DIGITAL MICROINJECTOR Built-in compressor with remote userinterface Two independent pressure channels Negative and positive pressure Two injection modes: pulse and continuous Transfer channel provides compensationpressure for high pressure injection Four range settings for transfer/compensation pressure Rotary controls for continuous pressureadjustment Four functions: Hold, Transfer, Inject, Clear170 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The XenoWorks ® Digital Microinjector is designed for transgenic mammal workstations,and adherent cell, C. elegans, drosophila, zebrafish, xenopus and sea urchin injections. It iscapable of holding oocytes and embryos while simultaneously providing a separate pressurechannel for pronuclear microinjection of DNA or the gentle control of embryonic stem cells as theyare transferred into blastocysts. The microinjector employs two independent pressure channels,Hold and Inject/Transfer, which are controlled via the remote user interface.The hold port is a self-contained vacuum channel for gentle suspension-cell holding and can beswitched on and off as needed. The responsive rotary control is used to dial the pressure up ordown according to the degree of hold required.MICROINJECTIONThe injector/transfer port is a second channel for delivering both high-pressure for femtoliter tomicroliter volume injections, and gentle positive/negative pressure for embryonic stem (ES) celltransfer. The transfer channel has four range settings to optimize the system to each applicationand user preference. It also includes a clear function which can be used if the pipette gets clogged.Two modes of injection, Continuous or Pulse, in combination with a balance pressure, are availableon this channel. In the continuous mode, the inject pressure is applied to the micropipette tip for aslong as the hand- or foot-switch is held down. In the Pulse mode, the inject pressure is applied fora precisely timed duration, which can be set in 0.01 second increments. Both injection durationand pressure are easily selectable using rotary controls on the remote interface. Injections areexecuted using the inject key on the remote interface, or the footswitch.Optimal ergonomics are again a prime consideration. The remote interface unit provides accessto all keys and rotary controls necessary to manipulate the parameters of the two pressurechannels, while the separate compressor controller can be positioned away from the microscopeto eliminate vibration.In addition to pronuclear injection, the XenoWorks Digital Microinjector can be used for a variety ofintracellular and extracellular applications including adherent and suspended cell microinjection.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM171

SPECIFICATIONSXENOWORKS ® DIGITAL MICROINJECTORMICROINJECTION Hold Function Inject Function Transfer Function Maximum Pressure Controls Micropipette Holder ElectricalHold pressure:-350 to +350hPa in 1hPa incrementsInject modes:Continuous or PulseInject pressure:0 to 5600 hPa in 7hPa incrementsCompensation pressure:Adjustable in 1hPa incrementsInject time:0 to 10 seconds in 0.01 sec incrementsClear pressure:Ramps from current Inject value to5600hPa at 1000hPa/secInject command:Remote interface or foot switchTransfer pressure:-175 to +175hPa in 1hPa increments5600hPa (Inject channel)Tactile keys, rotary optical encoders3 x 7-segment LED displayDiameter:4mmAccepts:1mm capillary glass2.4m ETFE tubing includedBRE110:100-120 Volts, 60HertzBRE220:220-240 Volts, 50 Hertz172 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICESXENOWORKS ® DIGITAL MICROINJECTORInternational prices are 5% higher. Prices subject to change without notice. BRE110 XenoWorks ® Digital Microinjector (110V) $ 7,900Includes: compressor module, footswitch, remote userinterface, 2 micropipette holders with 2.4m ETFE tubingand fittings, spare tubing, connecting cables, and manual BRE220 XenoWorks Digital Microinjector (220V) $ 7,900Includes: compressor module, footswitch, remote userinterface, 2 micropipette holders with 2.4m ETFE tubingand fittings, spare tubing, connecting cables, and manual BR-DT XenoWorks digital tubing kit $ 80 BR-MH XenoWorks micropipette holder $ 175MICROINJECTION(Remote user interface)PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM173

XENOWORKS ®ANALOG MICROINJECTORMICROINJECTIONFEATURESXENOWORKS ® ANALOG MICROINJECTOR Highly stable, drift-free Coaxial coarse and fine displacement(10:1 ratio) for fast filling and precision control Interchangeable syringes allowsensitivity to be modified Use with air, water, and oil Adjustable height control174 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

The XenoWorks ® Analog Microinjector has been designed with ease of use in mind. It is suitablefor air, water and oil, depending on the application and the level of responsiveness required. Ithas both fine and course displacement for fast filling and precise control and can be outfi ttedwith a syringe size of your choice. The design of the Analog Microinjector allows the tubing tobe easily separated from the syringe, providing convenient access for cleaning or replacing.Spare tubing and fi ttings are provided with each injector and the Luer-lock and ferrule connectorsassure quick and simple assembly. The tubing length can be altered according to the researcher’sneeds and ancillary devices, such as three-way valves, may be fi tted so that a single injectorcan control two micropipettes (for applications such as blastomere biopsy).MICROINJECTIONThe XenoWorks Analog Microinjector is the perfect tool for various applications including:intracytoplasmic sperm injection (ICSI), nuclear transfer (NT), embryonic stem (ES) cell transfer,holding, aspiration, and low pressure microinjection.BRI XenoWorks Analog Injector Volume ChartSYRINGE PART NUMBER SYRINGE VOLUME VOLUME PER REVOLUTION VOLUME PER REVOLUTIONCOURSE DIALFINE DIALV001180 10µl 162nl 16.2nlV001181 25µl 325nl 32.5nlV001182 50µl 650nl 65.0nlV001183 100µl 1.3µl 130nlV001184 250µl 2.6µl 0.26µlV001185 500µl 5.2µl 0.52µlV001186 1000µl 10.4µl 1.04µlPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM175

SPECIFICATIONSXENOWORKS ® ANALOG MICROINJECTORMICROINJECTION Coarse/Fine Ratio 10:1 Displacement/Turn Tubing Micropipette Holder500µl syringe:5.2µl coarse, 0.5µl fine1000µl syringe:10.4µl coarse, 1.04µl fine1.5m ETFEDiameter:4mmAccepts:1mm capillary glass(other sizes can be accommodatedupon request)176 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICESXENOWORKS ® ANALOG MICROINJECTORInternational prices are 5% higher. Prices subject to change without notice. BRI XenoWorks ® Analog Microinjector $ 2,060Includes: microinjector drive, 1 micropipette holderwith tubing and fi ttings, spare tubing, 500 microlitersyringe (100 or 1000 microliter syringe availableupon request) BR-MH XenoWorks micropipette holder $ 175 BR-AT XenoWorks analog tubing kit $ 55 BR-OIL XenoWorks oil for injector $ 40 V001180 10µl gas-tight syringe $ 110 V001181 25µl gas-tight syringe $ 110 V001182 50µl gas-tight syringe $ 110 V001183 100µl gas-tight syringe $ 110 V001184 250µl gas-tight syringe $ 110 V001185 500µl gas-tight syringe $ 110 V001186 1000µl gas-tight syringe $ 110MICROINJECTIONPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM177

PRIMETECH PMM-150FUPIEZO IMPACT DRIVEMICROINJECTIONSutter Instrument offers the PrimeTech PMM-150FU piezo impact drive for challenging microinjectionapplications such as mouse nuclear transfer, intracytoplasmic sperm injection (ICSI) and blastocystinjection. Oocyte plasma membranes, particularly those of the mouse, are particularly sensitive tomicroinjection, which tends to result in oocyte death. Initially designed for porcine oocyte injection,the PrimeTech PMM was adapted for use in the mouse in 1995 (Kimura, Y. and Yanagimachi, 1995),and since then has become the gold standard for mouse nuclear transfer and ICSI.The PMM-150FU harnesses the “piezoelectric effect”: the tiny, but extremely rapid change of apiezoelectric crystal lattice when an electrical current is applied to it. This causes the micropipetteholder, which is placed in direct contact with the piezo element to vibrate. Two modes of piezo“drilling” are available with the PMM-150FU: A continuous train of pulses of desired frequency andamplitude are used to vibrate the micropipette, gently penetrating the zona pellucida of an oocyte orembryo, then a single pulse, again of desired amplitude, is used to break the oolemma and inject theoocyte. This approach makes it possible to achieve up to 100% survival of injected oocytes.IncludedXenoWorks Micromanipulator-specific micropipette holder clampPrimeTech factory warrantyFull technical support for piezo impact applicationsKimura, Y. and R. Yanagimachi, 1995. Intracytoplasmic sperm injection in the mouse. Biol. Reprod. 52, 709-720178 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

US PRICES PMM-150FUInternational prices are 5% higher. Prices subject to change without notice. PMM-150FU Includes: Controller, operation box, piezo impact drive $ 15,900unit, micropipette holder, tubing and pressure fi ttings,and XenoWorks ® Micromanipulator adapter BR-BXW Replacement XenoWorks manipulator adapter $ 250 BR-LE Leica manipulator adapter for PMM-150FU $ 620 BR-NE Narishige manipulator adapter for PMM-150FU $ 620 V010200 PrimeTech injection holder IH-3 $ 60 V010210 Spacer set for IH-3. Includes steel collar, 10 x 6mm $ 60silicon seal V200240 Tubing set for IH-3. Includes 1m Tefl on tube, 2 x Luer $ 60connectorMICROINJECTION* Adapters for other brands of micromanipulator are also available.Available from Sutter in the Western Hemisphere. Please contactSutter for other international destinations.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM179

MICROINJECTIONAPPLICATIONSThe confi guration of any micro-injection system will depend upon the application for which it isused. Some common applications and system confi gurations are:Embryonic Stem Cell Transfer into BlastocystsAnimals, usually mice, can be engineered with a specifi c gene function reduced or knocked outaltogether by introducing genetically altered embryonic stem cells into the cavity of a blastocystso that the stem cells contribute to the embryo. The resulting live animal is a chimera of bothgenotypes. Subsequent selective interbreeding of manipulated animals results in pure-bredgene “knock-outs” or “knock-downs” and can be used for subsequent gene function studies.This operation requires two micromanipulators, one for holding the blastocyst and one fortransferring the cells. Both holding and transfer functions require gentle positive and negativepressure for which the Digital Microinjector is ideal. Alternatively, two AnalogMicroinjectors may be substituted.Suggested system confi guration:1 x Micromanipulator (Right) BRMR1 x Micromanipulator (Left) BRML1 x Digital Microinjector BRE110/BRE220or2 x Analog Microinjector BRI2 x Microscope Adapter180 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Zygote Pronuclear DNA MicroinjectionThe microinjection of DNA into the pronucleus of a newly-fertilized mammalian egg is now acommon and highly effi cient method of creating transgenic offspring.MICROINJECTIONPronuclear microinjection was first described in the mouse, but now many different transgenicanimals have been created in this way. Because the micropipette used for injection has an internaldiameter typically less than one micron, relatively high pressure (>3000 hPa) is required to injectthe DNA solution. Two micromanipulators are required, one to hold the zygote and one to injectthe DNA. Gentle negative pressure is used on the holding side, while pulses of high pressure areused to inject 1–2 picoliters of DNA solution into the pronucleus. The XenoWorks ® DigitalMicroinjector is ideal for this application, having simultaneous holding and high-pressureinjecting capabilities.Suggested system confi guration:1 x Micromanipulator (Right) BRMR1 x Micromanipulator (Left) BRML1 x Digital Microinjector BRE110/BRE2202 x Microscope AdapterPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM181

MICROINJECTIONAPPLICATIONSThe confi guration of any micro-injection system will depend upon the application for which it isused. Some common applications and system confi gurations are:Somatic Cell Nuclear TransferThe enucleation of an oocyte followed by the transplantation of a somatic cell is a method ofproducing genetically identical copies (clones) of the animal from which the donor cell wastaken. Generally, two micromanipulators are required: one for holding the oocyte and one for theenucleating and injecting procedures. Each micromanipulator grips a single micropipette holderwith a microinjector attached. Oocyte holding, enucleation and somatic cell transplantation requiregentle, low positive and negative pressure, making the Analog Microinjector ideal.Suggested system confi guration:1 x Micromanipulator (Right) BRMR1 x Micromanipulator (Left) BRML2 x Analog Microinjector BRI2 x Microscope Adapter182 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Intracytoplasmic Sperm InjectionIntracytoplasmic sperm injection (ICSI) can be employed for veterinary in-vitro fertilization duringrare species preservation or for any veterinary assisted conception. ICSI may also be used as agene transfer technique when sperm are co-injected with exogenous DNA (see also Piezo-assistedICSI, below). Typically, two micromanipulators are used, one for oocyte holding and one forsperm aspiration and injection. Each micromanipulator grips a single micropipette holder with amicroinjector attached. Since low positive and negative pressures are required for the delicatetask of oocyte holding and sperm injection, an ICSI workstation should be confi gured with twoAnalog Microinjectors.MICROINJECTIONSuggested system confi guration:1 x Micromanipulator (Right) BRMR1 x Micromanipulator (Left) BRML2 x Analog Microinjector BRI2 x Microscope AdapterPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM183

MICROINJECTIONAPPLICATIONSThe confi guration of any micro-injection system will depend upon the application for which it isused. Some common applications and system confi gurations are:Piezo-assisted ICSIThis relatively new technique can be employed for assisted conception in animals in which standardICSI fails, such as mice. Piezo-assisted micro-injection has also been employed as a gene transfermethod, where sperm are coated in exogenous DNA and injected into oocytes. The microinjectionworkstation required for this technique is very similar to standard ICSI, but with the addition of apiezo impact drive attached to the injecting micropipette holder. The device vibrates the injectingmicropipette axially and drills its way into the oocyte. This method has been shown to increasesuccess rates. Because the micropipette is vibrating at minute amplitude but high frequency, it isvital to use a mechanically stable micromanipulator, which will not vibrate in sympathy. The morestable the micromanipulator, the more effi cient the energy transfer from the piezo impact driveto the micropipette tip. Some piezo-assisted microinjection protocols currently require a bead ofmercury inside injecting micropipette. Please note that mercury should not be used in conjunctionwith the Digital Microinjector, though the Analog Microinjector is ideal forthis purpose.Suggested system confi guration:1 x Micromanipulator (Right) BRMR1 x Micromanipulator (Left) BRML2 x Analog Microinjector BRI2 x Microscope Adapter1 x PMM-150FU184 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

Microinjection of Cultured, Adherent CellsCultured cell lines such as 3T3, CHO and HeLa can be microinjected while attached to a Petri dish.The procedure is best viewed through phase-contrast optics; a single micromanipulator and asingle high-pressure microinjection channel are required. The tip of a sharp (inner diameter lessthan 1 micron) micropipette is brought down on top of a single cell and a pulse of high (100–1000 hPa) pressure applied. The cell membrane is ruptured and the cell can be seen to infl ateslightly. Volumes injected are typically less than 5% of the cell volume. Success rates vary widelydepending upon the type and volume of compound injected, the culture conditions and the cellline used. The high-pressure function of the Digital Microinjector and the smooth, finecontrol of the micromanipulator are particularly well-suited to this application.MICROINJECTIONSuggested system confi guration:1 x Micromanipulator (Right) BRMR1 x Digital MicroinjectorBRE110/BRE2201 x Microscope AdapterPlease contact Sutter Instrument for help and advice with these and any other microinjection needs.hPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM185

OEM PRODUCTSCUSTOM PRODUCTSCustomization of our optical product line for unique applications has become a specialty forSutter Instrument. Our production facilities include in-house electronic design, circuit board layout,embedded systems programming, and state-of-the-art CNC machining and turning centers forvolume production of precision mechanical components. This expertise gives Sutter the abilityto provide timely, cost-effective engineering and manufacturing solutions for Original EquipmentManufacturers (OEMs). The proven performance and reliability of our optical filter wheel systemshas made them a desirable choice for a range of OEM applications. Examples of custom designsinclude 5 position and 10 position wheels with 2 inch filters, 12 position arrays, compact belt-drivenfilterwheels, cryogenic and high vacuum filterwheels, rotating polarizers, an RS-485 serial busallowing 16 controllers to share a single serial port on a host computer, and mounting adaptersfor a wide range of microscopes. Sutter currently has several signifi cant OEM contracts and willcontinue to establish new OEM relationships. Please contact Sutter for more information aboutcustom filter changing devices.186 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

SAMPLE OEM PROJECTCUSTOM PRODUCTSA sample design project shown above: A cryogenic dual wheel with twinadjustable rotating polarizers for use in infrared astronomy.PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM187

INTERNATIONAL DEALERSINTERNATIONAL DEALERSA complete listing of international distributors and resellers in over 35 countries is available on ourweb site at www.sutter.com. Customers worldwide are welcome to purchase our products directly;however, for overseas sales, Sutter recommends that you purchase through one of our local distributors.AUSTRALIASDR Clinical Technology213 Eastern Valley Way,Middle Cove N.S.W. 2068, Australiaphone: +61-2-9958-2688fax: +61-2-9958-2655email: sdr@sdr.com.auweb: www.sdr.com.auCANADACarl Zeiss Canada Ltd.45 Valleybrook DriveToronto, OntarioCanada M3B 2S6phone: (416) 442-3322toll free: (800) 387-8037fax: (416) 446-3524web: www.zeiss.caNikon Canada Inc.Instrument Division1303 Aerowood Drive, Unit CMississauga, OntarioCanada L4W 2P6phone: (905) 602-9676fax: (905) 602-9953web: www.nikon.caOlympus Canada151 Telson RoadMarkham, OntarioL3R 1E7, Canadatoll free: (800) 387-0437phone: (905) 479-4100fax: (905) 479-5808web: www.olympuscanada.comHEKA Electronics Incorporated47 Keddy Bridge RoadMahone Bay, Nova ScotiaCanada B0J 2E0phone: (902) 624-0606fax: (902) 624-0310email: nasales@heka.comweb: www.heka.com188 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

CHINABioprobes Ltd.Rm. 116, JinXia Building,No.15 Shi You Xin Er Heng Road,Wu Yang Xin Cheng,Guangzhou, China P.C#510600phone: (020) 87357072, 87357737fax: (020) 87357072email: info@bioprobeschina.comweb: www.bioprobeschina.comDL Instruments - Wuhan284 Changqing Road, Hangtian Garden, Room 301-1-3BJianghan DistrictWuhan 430023, Chinatel: 86-27-83569708fax: 86-27-83569700email: dlwh@dongletech.comweb: www.dongletech.comINTERNATIONAL DEALERSDL Instruments - Shanghai669 Zhongshan West Road, Room 1-1201Changning DistrictShanghai 200335, Chinatel: 86-21-52738359fax: 86-21-52738360email: mailto:dlsh@dongletech.comweb: www.dongletech.comFRANCEBio-Logic SAS1, rue de l’Europe, Z.A. de Font-Ratel,F-38640 Claix, Francephone: +33 476 98 68 31fax: +33 476 98 69 09contact: www.bio-logic.info/e-form.phpweb: www.bio-logic.infoDipsi IndustrieImmeuble “Vecteur Sud”70-86 Avenue de la RepubliqueF-92325 Chatillon cedexFrancephone: +33 1 496 56 720fax: +33 1 496 56 729email: dipsi@dipsi.comweb: www.dipsi.comPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM189

INTERNATIONAL DEALERSGERMANYHEKA Elektronik GmbHWiesenstrasse 71,D-67466 Lambrecht/Pfalz, Germanyphone: +49 (0) 6325 9553-0fax: +49 (0) 6325 9553-50email: sales.eu@heka.com oremail: support@heka.comweb: www.heka.comScience Products GmbHHofheimer Str. 63D-65719 Hofheimphone: +49 (0) 6192 - 901396fax: +49 (0) 6192 - 901398email: info@science-products.comweb: www.science-products.comHONG KONGBioprobes Ltd.14/F. Highgrade Building,117 Chatham Road South,Tsimshatsui,Kowloon, Hong Kongphone: (852) 2723 9888fax: (852) 2724 2633email: info@bioprobes.imsbiz.com.hkweb: www.bioprobeshk.comINDIAMedi Analytika India Pvt. Ltd6, Adyar Bridge RoadAdyarChennai - 600 020Indiaphone: 044-2446 0988/2446 3084/2446 3085fax: 044-2446 3931e-mail: mediana@vsnl.comISRAELNBT Ltd.3 Makor Haim St.P.O. Box 8662JERUSALEM 91086, ISRAELphone: +97226732001fax: +97226731611email: nbtsales@nbtltd.comweb: www.nbtltd.com190 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM

ITALYJAPANCrisel Instruments, SRLVia Mattia Battistini, 177Rome 00167 Italyphone: +39 06 35402933fax: +39 06 35402879email: becattini@crisel-instruments.itweb: www.crisel-instruments.itShoshin EM CorporationShoshin Building, 1-14, Kuranishi,Akashibucho, Okazaki, 444-0241 Japanphone: +81 0564-54-1231fax: +81 0564-54-3207web: www.shoshinem.comemail: info@shoshinem.comINTERNATIONAL DEALERSNORWAYInter Instrument ASO.H. Bangs vei 511363 HovikNorwayphone: +47 67 10 79 60fax: +47 67 10 79 69mobile: 47 91 75 74 83contact: Dr. Anja-Rose Strohmaieremail: anja@interinst.noweb: www.interinst.noSWITZERLANDScience Products AGStoeberstr. 35CH-4055 Baselphone: +41-61-3033555fax : +41-61-3013570www.science-products.chTAIWANMajor Instruments Company, Ltd.9 Fl. 69-3, Chung-Cheng E. Road, Sec. 2,Tanshui, Taipei, TaiwanRepublic of Chinaphone: +886 (02) 2808-1452fax: +886 (02) 2808-2354email: major@major.com.twweb: www.major.com.twPHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COM191

INTERNATIONAL DEALERSUNITED KINGDOMINTRACEL LimitedUnit 4, Station Road, Shepreth, Royston,Herts, SG8 6PZ, Englandphone: +44 01763 262680fax: +44 01763 262676email: enquiries@intracel.co.ukweb: www.intracel.co.ukLinton InstrumentationNo. 11 Forge Business Centre,Upper Rose Lane,Palgrave, Diss. Norfolk, England 1P22 1APphone: +44 01379 651344fax: +44 01379 650970email: mail@lintoninst.co.ukweb: www.lintoninst.co.uk192 PHONE: 1-888-883-0128 • FAX: 1-888-883-0900INTL PHONE: 1-415-883-0128 • INTL FAX: 1-415-883-0572EMAIL: INFO@SUTTER.COM • WEB: WWW.SUTTER.COMPrinted in China