Marie Curie Workshop Laboratory Manual Clostridial Gene ...

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Centre for Biomolecular SciencesMarie Curie WorkshopSeptember, 2006Health and Safety InformationUniversity of Nottingham, Sept ’06 Page 24
IntroductionThe Centre for Biomolecular Sciences is a multi-disciplinary centre, bringing togetherresearchers from a wide range of different Schools and disciplines. A fundamentalreason for this venture is to create new collaborations between these disciplines andgroups, so that they can work together to take biomolecular research into exciting andfruitful new directions. An essential part of this process is ensuring the health andsafety of all of the staff, students and visitors working in the CBS. This guideprovides you with the basic health and safety information which you will need duringyour course at CBS. Specific health and safety information for the laboratories andtechniques which you will be using will be provided by the course tutors.SmokingSmoking is not permitted anywhere in the CBSAccess to CBSYou will normally be allowed access to CBS under the supervision of a CourseTutor between:0700 to 1900Access through the front door will be provided by the receptionist or a Course Tutor.You will be required to sign in and out of the building at Reception.When judged necessary by the Course Tutor, you will be allowed to remain in thebuilding under their supervision up to2145The building alarms activate automatically and the doors are locked at2200You must leave the building before that time.You will be issued with a key code to allow access to B floorDo not allow anyone else who is not known to you as authorised to enter CBS to gointo the building with you.University of Nottingham, Sept ’06 Page 25
Page 1 and 2: Marie Curie Workshop Laboratory Man
Page 3 and 4: FOREWORDIt is with great pleasure t
Page 5 and 6: ProgrammeMonday 11 th September - A
Page 7 and 8: Overview of practical sessionsDurin
Page 9 and 10: 3. Perform PCR using the following
Page 11 and 12: Ligation of the digested PCR produc
Page 13 and 14: Wednesday 13 th SeptemberTODAYPerfo
Page 15 and 16: The thiamphenicol will select for t
Page 17 and 18: Wash cells and allow recovery perio
Page 19 and 20: 1. Label PCR tubesLabel 8 PCR tubes
Page 21 and 22: Inspect plates and interpret result
Page 23: PARTICIPANTSSURNAME FIRST NAME INST
Page 27 and 28: If you hear the fire/evacuation ala
Page 29 and 30: Good Laboratory PracticeWithin the
Page 31 and 32: Waste DisposalAll hazardous biologi
Page 33 and 34: Mixed biological and chemical waste

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