Pest risk assessment of the Cucumber green mottle

More documents

Recommendations

Info

07/207-4 FinalJapanese cucumber strain has been described from Japan. This strain gives severe fruitsymptoms in cucumber and local lesions in D. stramonium, but no local lesions in C.amaranticolor. This strain is now classified as a separate species, KGMMV.The formerly described Yodo strain gives severe fruit symptoms in cucumber and locallesions in C. amaranticolor, D. stramonium and P. x hybrida. This strain is now classified asKGMMV-Y (Francki et al. 1986).The “Indian strain” was isolated from bottlegourd(Lagenaria siceraria) in India (Hollings et al. 1975), in which it caused mosaic, growthreduction and yield loss. This isolate is reported to give local lesions in C. amaranticolor,symptomless infection in inoculated leaves of D. stramonium and no infection in tobacco orP. x hybrida.Of the 22 definitive members in the genus Tobamovirus, four have been identified in Norway(Blystad and Munthe 1997):• Cucumber green mottle mosaic virus (agurkgrønnmosaikkvirus) in cucumber• Ribgrass mosaic virus (smalkjempemosaikkvirus) in Plantago major• Tobacco mosaic virus (tobakkmosaikkvirus) in petunia• Tomato mosaic virus (tomatmosaikkvirus) in tomato4.1.1.5 Bayer computer codeCGMMV4.1.2 Methods for detection and identification4.1.2.1 Symptoms in cucumberTypical symptoms of CGMMV such as mosaic, leaf bubbling, wilting and chlorosis are easilyrecognized, but symptoms on lower leaves and weak symptoms may look like physiologicaldisorders.Norwegian Scientific Committee for Food Safety 8
07/207-4 FinalFigure 1. Symptoms of CGMMV consisting of mosaic (upper left) (photo: Erling Fløistad), leaf bubbling(upper right) (photo: Dag-Ragnar Blystad), wilting (lower left) (photo: Dag-Ragnar Blystad) and chlorosis(lower right) (photo: Ørjan Omdal).4.1.2.2 Test plantsThe host plant cucumber (Cucumis sativus) can be used as a test plant. The time frominoculation to symptom development is 14 days.CGMMV infects very few of the commonly used test plants in plant virology. The test plantsC. amaranticolor, D. stramonium, Nicotiana benthamiana and P. x hybrida have been used todescribe and differentiate isolates.4.1.2.3 Electron microscopyThere are relatively high amounts of CGMMV particles in the plant sap of infected leaves.This gives the possibility to use negative staining as a rapid method to assess the occurrenceof CGMMV particles. These particles are typical for tobamoviruses with the dimensions 300x 18 nm.Norwegian Scientific Committee for Food Safety 9
Page 1 and 2: 07/207-4 FinalOpinion of the Panel
Page 3 and 4: 07/207-4 FinalCONTENTS1. Background
Page 7: 07/207-4 Final4.1.1.2 SynonymsCucum
Page 11 and 12: 07/207-4 FinalThe situation in othe
Page 14 and 15: 07/207-4 FinalA new cucumber cultur
Page 16 and 17: 07/207-4 Final4.1.7.3 Dissemination
Page 18 and 19: 07/207-4 FinalGreenhouse cucumber(s
Page 20 and 21: 07/207-4 FinalPeopleThe grower, con
Page 22 and 23: 07/207-4 Final• Use of milk (dipp
Page 24 and 25: 07/207-4 Final- Outbreaks are manag
Page 26 and 27: 07/207-4 Finaland periods with no p
Page 28: 07/207-4 FinalNilsson B. 1977. Unde

Pest risk assessment of the Cucumber green mottle

Create successful ePaper yourself

Delete template?

Save as template?