QuickGene mini 80 - Lab Services BV
QuickGene mini 80 - Lab Services BV
QuickGene mini 80 - Lab Services BV
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Personal nucleic acid isolation device, one for<strong>QuickGene</strong>-Mini<strong>80</strong> is a compact system requiring no centrifugation in the extraction process, giving less strain to samples and enablingrapid nucleic acid extraction. DNA/RNA can be easily extracted from various samples including human/mouse/plants/cells/fungi andothers. The <strong>QuickGene</strong>-Mini<strong>80</strong> shares a common extraction mechanism and kits with our semi-automated nucleic acid isolationsystem, <strong>QuickGene</strong>-810. <strong>QuickGene</strong>-Mini<strong>80</strong> offers high performance comparable to <strong>QuickGene</strong>-810 at a reasonable price.Compact DesignThe small, lightweight <strong>QuickGene</strong>-Mini<strong>80</strong> takes up <strong>mini</strong>malspace on the lab bench and is easy to carry.<strong>QuickGene</strong>-Mini<strong>80</strong> can be operated on the lab bench so, withoutmoving around, the user can perform nucleic acid extraction.Revolutionary Porous MembraneThe <strong>QuickGene</strong>-Mini<strong>80</strong> uses FUJIFILM'spatented porous membrane only <strong>80</strong>µm thick.Porous membraneGlass fiber membraneBecause of the outstanding absorptive anddesorptive performances of the membrane,high-purity nucleic acid can be easily obtainedin high yield at low pressure.The ultra thin membrane enables nucleic acidextraction in shorter time than when comparedto glass fiber membranes.Cartridge50µm50µmHigh Purity, High YieldHighly reproducible <strong>QuickGene</strong>-Mini<strong>80</strong> can stably extract nucleic acid in high yield.The extracted DNA/RNA contains few impurities and can be used directly in PCR and RT-PCR.Length of extracted DNAExtraction of plasmid DNAExtraction of DNA fromtissueExtraction of RNA fromtissue<strong>QuickGene</strong> DNA tissue kit S <strong>QuickGene</strong> Plasmid kit S <strong>QuickGene</strong> DNA tissue kit S<strong>QuickGene</strong> RNA tissue kit SM1M2 M3TailLiverMpBS /GAPDH/DH5 (1×10 9 )MTailMLiverLiver Spleen HeartDNase DNase DNaseM + - + - + -190-260145kb121kb97kb73kb48kb24kb23.1kb9.4kb6.6kb4.4kbM :1kb PLUS DNA Ladder M :1kbp PLUS DNA Ladder M :1kbp PLUS DNA LadderYield and purity of plasmid DNAYield and purity of genomic DNAYield and purity of total RNA2.0kbM1: Hind digestM2:MidRange PFG MarkerM3:Mouse Genomic DNA(BD)∗Balb/c mouse (female, 7week old)Average*DNA yield(µg) 23.0Purity(260/2<strong>80</strong>) 1.97Purity(260/230) 2.28*Average of 4 samplesDNA yield(µg)Purity(260/2<strong>80</strong>)Purity(260/230)Tail4.01.9162.117Liver3.61.9251.942∗Balb/c mouse (female, 7week old)∗Without RNase treatmentDNase Livertreatment 32mg+ 155.4total RNA yield(µg)- 159.3+ 2.1Purity(2<strong>80</strong>/260)- 2.1+ 2.3Purity(2<strong>80</strong>/230)- 2.2∗Balb/c mouse (female, 7week old)Spleen32mg71.8Heart32mg21.274.3 21.62.12.12.22.22.1 2.02.1 1.8
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