Risk Group and Biosafety Level Containment - University Research ...

Risk Group and Biosafety Containment LevelRisk Group(NIH Guidelines)Biosafety Containment LevelExamplesRisk Group 1:Agents are NOTassociated with diseasein healthy adult humans.(Low risk)Risk Group 2:Agents are associatedwith human diseasewhich is rarely serious.There are oftenpreventive ortherapeutic interventionsavailable.(Moderate risk)Risk Group 3:Agents are associatedwith serous or lethalhuman disease for whichpreventive ortherapeutic interventionsMAY be available.(High risk)Risk Group 4:Agents are likely tocause serious or lethalhuman disease for whichpreventive ortherapeutic interventionsare NOT USUALLYavailable.(Extreme risk)BSL-1BSL-2BSL-3BSL-4Work is done on open bench tops and specialcontainment equipment is not requiredStandard microbiological practices are observedAll BSL-1 containment and practices plus the following:Laboratory access is restricted when experimental workis in progressPersonnel have specific training in handling of agentsBiological safety cabinets (BSC) or other physicalcontainment devices are used for potential aerosolgeneration proceduresBiohazard signs must be postedSpecific PPE (personnel protective equipment) andentrance requirementsAll BSL-2 containment and practices plus the following:Specific facility design parameters must be followed,including requirements for location, ventilation, roomintegrity and securityNO current facilities exist to accommodate risk group 4agents at Oklahoma State University.Escherichia coli; K12derivatives (DH5a,JH109, pBluescript,psi2); All EXEMPTrDNA work• Adenovirus alltypes; human• All humanbloodcontaminatedspecimens:HIV/SIVinfected animals• Human celllines eg. HEK293• Herpes Simplex Virus• Rabies Virus• Pseudorabies Virus• Bartonella• Coxiella burnetii• Mycobacterium bovis• Poxviruses• Retroviruses• Arenaviruses• Filoviruses

Risk Group and Biosafety Containment Level for Animal,Plant, or rDNA not related to risk in HumansRisk Group(NIH Guidelines)Risk Group 1:Experiments that do notpose a risk to theenvironment – releasewould not result insurviving in theenvironment. (Low risk)Risk Group 2:Experiments that involvework agents or transgenicsthat if released would beviable in the environmentbut would have anegligible impact or couldbe readily managed.(Moderate risk)Risk Group 3:Experiments that a releaseoutside the lab would havesignificant detrimentalimpact on the environment.(High risk)Risk Group 4:Experiments with exoticsthat are serious pathogensof major US crops andagriculture, and wouldhave a devastating impacton the environment.(Extreme risk)BSL-1BSL-2BSL-3BSL-4Biosafety Containment LevelWork is done on open bench tops and specialcontainment equipment is not requiredStandard microbiological practices are observedAll BSL-1 containment and practices plus thefollowing:Laboratory/Facility/Greenhouse access is restrictedwhen experimental work is in progressPersonnel have specific training in handling of agentsBiological safety cabinets (BSC) or other physicalcontainment devices are used for potential aerosolgeneration proceduresBiohazard signs must be postedSpecific PPE (personnel protective equipment) andentrance requirementsAll BSL-2 containment and practices plus thefollowing:Specific facility design parameters must be followed,including requirements for location, ventilation, roomintegrity and securityNO current facilities exist to accommodate risk group4 agents at Oklahoma State University.ExamplesEscherichia coli; K12derivatives (DH5a,JH109, pBluescript,psi2); All EXEMPTrDNA work;Rhizobium,Agrobacterium• rDNA work on plantsthat could becomeestablished if released• Potentially harmfulmicroorganisms toother animals or plantsbut that are manageable•Exotics that pose nopotential harm tomanaged or naturalecosystems• Herpes Simplex Virus• Rabies Virus• Pseudorabies Virus• Exotic infectiousagents capable ofcausing seriousenvironmental harm• Plants containinggenes from exoticinfectious agents• Mycobacterium bovis