Analytical Interferences and Physiological Limitations of Blood ...

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The pO 2 effectGOglucose + O 2 + H 2 O gluconic acid + H 2 O 2HRPOH 2 O 2 + dye precursor dye color + H 2 0(YSI <strong>and</strong> BeckmanGlucose Analyzer)(colorimetric)glucose + med (ox)GOgluconolactone + med (red)(electrochemical)med (red)E pote - + med (ox)03162010 Ken Ervin Consulting Services
How a pO2 interference became amaltose interference Original methods based on glucose oxidase coupledto a colorimetric indicator system.Oxygen available from atmosphere blood removed by blotting, wiping etc. exposed to air during the reaction time Electrochemical methods used mediatorsSystems calibrated for capillary blood Oxygen would interfere competitivelyUse <strong>of</strong> venous or arterial blood exacerbated this competition Venous reads higher; less 0 2 competition Arterial reads lower; more 0 2 competitionpO2 effects generally greater at lower glucose concentrations03162010 Ken Ervin Consulting Services
Page 1 and 2: Analytical Interferences andPhysiol
Page 3 and 4: Package inserts address “Procedur
Page 5 and 6: BGM Design GoalsDrive the specifica
Page 7 and 8: BGM measurement based oncombining t
Page 9 and 10: Interferences result from Analyte s
Page 11 and 12: Sample influences on measurement En
Page 13 and 14: Environmental influences Analytical
Page 15: Some relevant examples How a pO 2 d
Page 19 and 20: How a pO2 interference became amalt
Page 21 and 22: Hematocrit dependenceLittle method
Page 23 and 24: In Conclusion Limitations and inter
Page 25 and 26: 03162010 Ken Ervin Consulting Servi
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Analytical Interferences and Physiological Limitations of Blood ...

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